32 results about "Genetic recombination" patented technology

The invention relates to the technical field of data processing, and discloses a heterogeneous computing acceleration method and system based on a deep learning framework network. The method comprises the following steps: acquiring performance parameters of heterogeneous computing equipment to obtain an equipment characteristic data set; receiving a calculation task and analyzing the calculation task into an operation sequence; the operation sequence is coded into a gene sequence, task decomposition is optimized through a genetic recombination algorithm, and a subtask set marked with acceleration characteristics is obtained; performing matching analysis on the sub-task set and the equipment characteristic data set to obtain a task allocation scheme; deploying the subtasks to corresponding equipment according to the allocation scheme to obtain a distributed execution framework; and monitoring the running state of the framework in real time, and dynamically adjusting resource allocation to obtain a calculation result of speed-up ratio improvement. According to the method, a self-adaptive task decomposition and resource allocation and dynamic load balancing mechanism can be realized, and the execution efficiency and the resource utilization rate of the deep learning task are improved.

The invention relates to recombinant saccharomyces cerevisiae for de novo synthesis of mevalonic acid as well as a construction method and application of the recombinant saccharomyces cerevisiae, and belongs to the technical field of genetically engineered bacteria. The technical problem to be solved by the invention is to provide the recombinant saccharomyces cerevisiae for de novo synthesis of mevalonic acid. According to the recombinant saccharomyces cerevisiae, saccharomyces cerevisiae is used as a chassis strain, and ACL, CTP1, RtME and MDH3 are over-expressed. A series of recombinant saccharomyces cerevisiae strains with high yield of mevalonic acid are constructed through a gene recombination method, the strains can be fermented in a conventional culture medium to prepare mevalonic acid, the fermentation process is simple, the cost is low, the yield is high, and the maximum yield of mevalonic acid can reach 5692.45 mg / L. According to the invention, the recombinant saccharomyces cerevisiae strain is used as a chassis strain, and recombinant genetically engineered bacteria for producing the santalene, the santalol and the patchouli alcohol are respectively obtained through gene recombination, so that the yields of the santalene, the santalol and the patchouli alcohol produced by fermentation are improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker-based rice disease resistance character whole genome association analysis method, which relates to the technical field of biology, and is characterized by comprising the following steps: selecting a representative genetic recombination population, carrying out high-density SNP genetic typing on the population, and combining recombination event positioning and linkage analysis to obtain the disease resistance character whole genome association analysis of rice. Obtaining genetic parameters reflecting recombination frequencies of different genome segments; on the basis of the obtained original genetic data, parent and offspring genotype information is further integrated, fine positioning and statistics are carried out on recombination breaking points, and a high-resolution genetic map is constructed. By constructing a high-resolution genetic map and combining feature engineering and machine learning, accurate identification and dynamic SNP encryption of a high-recombination region are realized, the marker coverage and associated signal capture capability of the region is enhanced, the functional site leak detection risk is reduced, and the detection efficiency is improved. The positioning accuracy and the molecular breeding application effect of the rice disease resistance character related candidate gene are improved.

The invention discloses a recombinant strain for efficiently expressing xanthine oxidase as well as preparation and application of the recombinant strain, and belongs to the technical field of enzyme engineering. According to the recombinant strain, four kinds of recombinant Pseudomonas putida strains are designed, and the strains can be used for efficiently expressing xanthine oxidase. The recombinant strain has the advantages of high expression quantity, simple affinity purification process flow, high purification yield and low production cost, and the recombinant xanthine oxidase has the specific activity similar to that of xanthine oxidase sold in the market at present and is suitable for various applications. The recombinant strain can realize high-efficiency expression of xanthine oxidase, and high-efficiency expression of xanthine oxidase is realized in P.putida through a gene recombination technology; the expressions are induced by adding an inducer, so that the maximum expression quantity of the xanthine oxidase is realized.

The invention provides a GMRM (Genome-based Mutant RNA Mapping) method taking a genome as a reference, in particular, the invention provides a method for cloning candidate functional genes of wheat stripe rust resistant plants and an analysis process, and the GMRM method disclosed by the invention can be used for simultaneously cloning two genes with complementary disease-resistant functions, namely Yr6NLR1 and Yr6NLR2. In an example of Yr6, the two disease-resistant genes are closely linked genetically, but the cloning process of the genes does not involve any genetic recombination. Therefore, theoretically, the method provided by the invention can be used for simultaneously cloning a plurality of functional complementary genes (namely genes participating in the same pathway) on the premise that the target gene can generate effective mutation through EMS mutagenesis.

The present invention provides a method for producing a culture strain that can be subcultured under conditions suitable for genetic recombination. (1) a culture step of culturing a plant piece to obtain a seedling; (2) a size confirmation step of confirming the size of the obtained seedlings; (3) The size of the seedling confirmed in the size confirmation step is (A) If the threshold is exceeded, the young plants are treated by removing dead leaves and roots, and the resulting culture is used for subsequent propagation. (B) If the concentration is below the threshold, the young plants are treated by leaving all healthy leaves and roots, and are used as culture lines for subsequent generations. a subculture preparation step; A method for producing a culture strain having the compound of formula (I) which is subcultured.

The invention provides genetically engineered saccharomycetes capable of simultaneously producing baicalein and baicalin as well as a construction method and application of the genetically engineered saccharomycetes. According to the invention, by weakening or deleting a glucose 5-phosphate dehydrogenase gene (ZWF1) and phenylpyruvate decarboxylase genes ARO10 and PDC5 in a saccharomyces cerevisiae genome and enhancing the expression of a pre-benzoic acid dehydratase gene Pha2, the metabolic flow of a shikimic acid pathway is increased, the aldrin pathway is blocked, and the yield of the shikimic acid pathway is increased. Therefore, the yield of L-phenylalanine serving as a starting material for synthesizing baicalein and baicalin is increased; meanwhile, exogenous genes PAL, 4CL, CHS, CHI, FNSII, F6H, CPR and F7GAT are integrated onto the genome by virtue of a gene recombination technology, so that baicalein and baicalin are produced from the beginning by taking glucose and glycerol as a mixed carbon source without adding precursor substances such as exogenous L-phenylalanine or malonyl CoA and the like.

The present invention provides a novel marker for genetic recombination in Starmerella bombicola and a method for using the same. An adenine-requiring selection marker consisting of a gene encoding a phosphoribosylglycine amidoformyltransferase-like protein or a gene equivalent thereto. A Starmerella bombicola mutant strain in which the expression of a phosphoribosylglycine amidoformyltransferase-like protein or a protein equivalent thereto is suppressed or inactivated.

The invention discloses a Trichoderma reesei engineering strain for producing trans-aconitic acid as well as a construction method and application of the Trichoderma reesei engineering strain, and relates to the technical field of biological engineering. The construction method comprises the following steps: transforming a coding gene of mitochondrial tricarboxylic acid transport protein and a coding gene of aconitic acid isomerase into a trichoderma reesei host bacterium, and carrying out gene recombination construction to obtain the trichoderma reesei engineering strain, the amino acid sequence of the mitochondrial tricarboxylic acid transporter is as shown in SEQ ID NO. 1; the amino acid sequence of the aconitic acid isomerase is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. A new production strain is provided for microbial fermentation production of trans-aconitic acid, a large amount of trans-aconitic acid can be produced through direct fermentation by using common carbon sources such as glucose or cellulose, and the highest yield can reach 26.5 g / L.

The invention is applicable to the field of gene engineering, and provides a mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle is formed by sequence fusion of mycobacterium tuberculosis Ag85B protein, TB8.4 protein and LS protein, and the amino acid sequence of the mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle is shown as SEQ ID NO: 2 in a sequence table. According to the invention, by virtue of a gene recombination technology, key immunogens Ag85B and TB8.4 of mycobacterium tuberculosis are combined with an LS protein fusion vector, and the Ag85B-TB8.4-LS protein nanoparticles with uniform particle size and stable structure are efficiently produced by virtue of an escherichia coli expression system. Animal experiment results show that the Ag85B-TB8.4-LS protein nanoparticle can simultaneously excite strong humoral immunity and cellular immunity response, not only brings a new idea for research and development of tuberculosis vaccines, but also can be popularized and applied to research and development of other infectious disease vaccines due to the modular design, and has important scientific significance and industrialization prospects.

The present application relates to the technical field of plant breeding, and particularly relates to a method for evaluating the double haploid induction potential of parent combinations in plant breeding. The present application constructs a method for evaluating the double haploid induction potential of parent combinations in plant breeding through genetic recombination simulation, a phenotype prediction model and a comprehensive evaluation function of multi-trait collaborative selection, can quantitatively predict the induction potential of different parent combinations before induction, can simultaneously consider multiple breeding objectives, realize collaborative optimization, and thus help to prioritize the allocation and scale planning of breeding resources. The present application can effectively save induction and identification resources, accelerate the breeding process of excellent varieties, and has a wide application prospect in the breeding of corn, rice, wheat and other plants.

The present invention discloses a method for promoting chondrocyte growth, comprising the following steps: (1) constructing BMP-2 gene recombinant MSC (MSCdBMP2) cells to express BMP-2 protein on the surface of MSCdBMP2 cells; (2) preparing exosomes (MSCdBMP2Exo) derived from MSCdBMP2; (3) stimulating MSCdBMP2 cells and MSCdBMP2Exo to significantly promote chondrocyte growth. The present invention clones the BMP-2 gene into MSC cells through genetic recombination technology to prepare new BMP-2 gene recombinant MSC (MSCdBMP2) cells; at the same time, the exosomes (MSCdBMP2Exo) prepared from MSCdBMP2 cells have a biological function similar to that of MSCdBMP2 cells in promoting chondrocyte growth.

The invention relates to the technical field of material chemical industry, in particular to a method and a system for extracting natural pigment for coating based on microbial fermentation, which comprises the following steps: acquiring a plurality of initial strains, acquiring a genetic recombination strain group, preparing single-cell suspension from the genetic recombination strains, performing fluorescence detection on the single-cell suspension, and confirming an optimal mutant strain. The method comprises the following steps: obtaining a mutant strain pigment crude extract, confirming a maximum absorption wavelength, culturing an optimal mutant strain, respectively carrying out absorbance determination on each mutant strain experimental group to obtain an absorbance value set, confirming an optimal fermentation temperature, carrying out large-scale fermentation to obtain a high-secretion fermentation liquid, carrying out wall breaking treatment to obtain a wall-broken thallus suspension, and carrying out freeze drying to obtain the high-secretion thallus suspension. The method comprises the following steps: preparing an intracellular pigment solution, carrying out microencapsulation on the intracellular pigment solution to obtain pigment microspheres, extracting a verification pigment extracting solution from the pigment microspheres, and obtaining the natural pigment by utilizing the verification pigment extracting solution. The problems of low extraction efficiency, high energy consumption and environmental pollution during extraction of the natural pigment for the coating can be solved.

This invention relates to the field of underwater adhesive materials, and particularly to an injectable self-healing underwater protein and its uses. The invention provides a fusion protein comprising a mussel byssal protein fragment and a coiled-helical structure fragment. This invention involves genetic recombination of mussel byssal protein and the coiled-helical structure, thereby providing a novel fusion protein that can self-assemble in solution to form a gel material, possessing both the interfacial adhesiveness of mussel byssal protein and the intrinsic adhesiveness of the coiled-helical structure, thus achieving super-strong underwater adhesion.

The present invention provides a novel marker for genetic recombination in Starmerella bombicola and a method for using the same. The present invention also provides a Starmerella bombicola mutant strain in which the expression of an orotate phosphoribosyltransferase-like protein or a protein equivalent thereto is suppressed or inactivated, a uracil-requiring selection marker consisting of the gene, and a method for producing a Starmerella bombicola transformant using the selection marker.

The present application relates to the field of data processing technology, and discloses a heterogeneous computing acceleration method and system based on a deep learning framework network. The method includes: collecting performance parameters of heterogeneous computing devices to obtain a device characteristic data set; receiving computing tasks and parsing them into operation sequences; encoding the operation sequences into gene sequences, optimizing task decomposition through a genetic recombination algorithm, and obtaining a subtask set marked with acceleration characteristics; matching and analyzing the subtask set with the device characteristic data set to obtain a task allocation scheme; deploying the subtasks to the corresponding devices according to the allocation scheme to obtain a distributed execution framework; monitoring the framework operation status in real time, dynamically adjusting resource allocation, and obtaining a calculation result with an improved acceleration ratio. The present application can realize adaptive task decomposition and resource allocation and dynamic load balancing mechanism, thereby improving the execution efficiency and resource utilization of deep learning tasks.

Provided are a novel genetic recombination marker of Starmerella bombicola, and a method for using the same. An adenine-requirement selectable marker comprising a gene encoding a phosphoribosylglycinamide formyltransferase-like protein or an equivalent gene. A mutant strain of Starmerella bombicola in wich the expression of a phosphoribosylglycinamide formyltransferase-like protein or an equivalent protein is suppressed or inactivated.

The present invention relates to the technical fields of fermentation engineering and genetic engineering, and particularly relates to a method for synthesizing lactic acid monomer using sucrose as a raw material. Through the genetic recombination technology of site-directed gene replacement, the promoter sequence of the sucrose metabolic operon in the production strain is corrected, enabling it to maintain a high level of expression throughout the production process of lactic acid monomer, demonstrating an obvious ability to highly catabolize sucrose to synthesize lactic acid monomers with high optical purity and high chemical purity (D-lactic acid or L-lactic acid). For example, strains DSAPW5 and LSAPW5 are fermented for 28-30 h under the condition of 25-50 °C, and the fermentation levels of D-lactic acid and L-lactic acid can reach 158.58 g / L and 159.65 g / L respectively, and the optical purity can reach 99.94% and 99.95%. The present invention can be applied to the industrial production of lactic acid monomer using sucrose or sucrose-containing raw materials.

The present application relates to the field of genetic engineering, in particular to a plant constitutive expression promoter and application thereof. The plant constitutive expression promoter provided by the present application is selected from the DNA sequence shown in any one of SEQ ID NO. 1-6. The plant constitutive expression promoter provided by the present application can regulate gene expression in plants after genetic recombination, and has significant value in practical application. Through genetic modification of crop varieties by the promoter, such as regulating the expression of target genes in plants by the promoter, ideal transgenic plant varieties can be cultivated.

The present invention provides: a novel marker for genetic recombination of Starmera bombicola; and a method for using the same. The present invention pertains to: a Starmerella bombicola mutant strain in which expression of an orotate phosphoribosyltransferase-like protein or an equivalent protein is suppressed or the protein is inactivated; a uracil auxotrophic selectable marker comprising the gene; and a method for producing a transformant of Starmerella bombicola using the selectable marker.

The invention relates to the technical field of plant breeding, in particular to a method for evaluating the double haploid induction potential of a parent combination in plant breeding. The method for evaluating the induction potential of the double haploids of the parent combination in plant breeding is constructed through a comprehensive evaluation function of genetic recombination simulation, a phenotype prediction model and multi-character collaborative selection, the induction potential of different parent combinations can be quantitatively predicted before induction, multiple breeding targets can be considered at the same time, collaborative optimization is achieved, and the method is suitable for large-scale popularization and application. Therefore, the priority allocation and scale planning of breeding resources are facilitated. The method can effectively save induction and identification resources, accelerates the breeding process of excellent varieties, and has wide application prospects in breeding of plants such as corn, rice and wheat.

A method for producing self-rejuvenating and safe cells, and a self-rejuvenating and safe repair cell are provided. [Solution] A technology and its use are provided for controlling the Jak-Stat pathway to differentiate, dedifferentiate, and rejuvenate cells. By quantitatively and / or periodically controlling gene or protein targets of the Jak-Stat signaling pathway in cells using combinations of small molecule compounds, cytokines, or recombinant proteins, gene editing technology, or genetic recombination technology, rejuvenated cell products and / or different types of cell products are obtained. This product can be applied to the reprogramming of cells, tissues, organs, and living organisms; the construction of tissue engineering materials; the repair of damaged and aging, degenerated tissues and organs in mammals; the delay or reversal of the progression of aging in cells, tissues, organs, and living organisms; and the immunomodulation of cells, tissues, organs, and living organisms.

The present invention discloses a genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid by taking glucose as a substrate and applications thereof, and belongs to the technical field of genetic recombination and metabolic engineering. The genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid (MA) by taking glucose as the substrate disclosed in the present invention is modified with chassis microbes, and includes recombinant Corynebacterium glutamicum for a cis,cis-muconic acid pathway construction module and an intermediate high-yield module. Production capacity of strains is greatly improved; MA of 90.2 g / L is finally obtained in fermentation liquor; and possibilities are provided for green and low-cost production of numerous chemicals such as adipic acid and nylon-66.

The invention belongs to the technical field of biology, and particularly relates to a method for improving rice genetic recombination frequency. The HEI10 gene is over-expressed in rice, and a rice plant or a rice variety with improved genetic recombination frequency is obtained through screening. According to the invention, a scheme for improving the genetic recombination frequency is established in rice through a transgenosis method instead of a knockout method. Transgenic components of the gene can be screened and removed in offspring through genetic isolation, so that the genetic recombination frequency of the offspring is recovered to a normal level, and the stability of genome and maturing rate is facilitated. And the overexpression of the HEI10 basically does not influence the normal growth and the maturing rate, so that the method has practical significance.

The invention belongs to the technical field of gene engineering, and particularly relates to a gene OsMCS1 for regulating and controlling meiosis of rice as well as an encoding protein and application of the gene OsMCS1. The nucleotide sequence of the gene OsMCS1 is as shown in SEQ ID NO. 1. Through knockout of the gene OsMCS1 in the rice, the rice chromosome separation after gene knockout is abnormal, the embryo sac is partially aborted, the chromosome recombination frequency is improved by 1.5-2.0 times compared with that of a wild type and a knockout line, and the genetic recombination frequency is improved, so that the breeding efficiency is improved, and a new means is provided for improving the rice genetic breeding efficiency.

The present invention provides a recombinant genetically engineered bacterium for producing phytosphingosine, and its preparation method and use, belonging to the field of genetic recombination fermentation technology. The recombinant genetically engineered bacterium of the present invention is obtained by overexpressing the serine palmitoyltransferase LCB1 gene, LCB2 gene, tsc3 gene, 3-dehydrodihydrosphingosine reductase tsc10 gene, sphingosine hydroxylase sur2 gene, serine integrin tms1 gene, phosphoglycerate dehydrogenase ser3 gene in the genome of Saccharomyces cerevisiae, and knocking out the sphingosine kinase phospho-LCB4 gene, ceramide synthase LAG1 gene and LAC1 gene, LDB16 gene, sphingolipid homeostasis protein orm1 gene and orm2 gene, L-serine deaminase CHA1 gene, L-serine hydroxymethyltransferase SHM1 gene and SHM2 gene, and fatty acid elongase elo2 gene. The method for producing phytosphingosine using the recombinant genetically engineered bacterium is cost-effective, simple and efficient, with high phytosphingosine yield, and suitable for practical promotion and application.

Provided are a novel marker for gene recombination of Starmerella bombicola and a method for using the same. The present invention provides an adenine auxotrophic selection marker consisting of a gene encoding a phosphoribosyl-glycinamide formyltransferase-like protein or a gene equivalent thereto. The present invention also provides a Starmerella bombicola mutant strain in which expression of a phosphoribosyl-glycinamide formyltransferase-like protein or a protein equivalent thereto is suppressed or the protein is inactivated.

The present disclosure provides compositions, methods, and systems related to genome editing technology. In particular, the present disclosure provides a novel CRISPR-based genome editing technology that involves the generation of abasic sites to facilitate genetic recombination, without the need for breaks in the DNA. The compositions, methods, and systems described herein address many of the drawbacks of currently available approaches, including off-target effects and cellular toxicity.

The present invention relates to a biopharmaceutical material and application of co-expressed porcine interleukin 15, 21 and 23, and relates to the field of biotechnology. The present invention provides a fusion protein comprising porcine interleukin 15, porcine interleukin 21 and porcine interleukin 23, consisting of an amino acid sequence as shown in SEQ ID NO: 1. A nucleic acid molecule encoding the fusion protein. A biopharmaceutical material comprising an expression cassette, a recombinant vector, a transfected cell, a recombinant microorganism, a culture product of the transgenic cell line or a fermentation product of the recombinant microorganism having the nucleic acid molecule. The present invention obtains a fusion protein by genetic recombination and fusing porcine interleukin 15, 21 and 23 genes, connecting them to a eukaryotic expression platform, and finding that the fusedly expressed IL-15, 21 and 23 have good biological effects of significantly enhancing the mucosal immune barrier function of animals, enhancing their systemic immunity level and anti-bacterial infection ability, and promoting the growth and development of animals.

The present invention discloses a porcine monoclonal antibody against the SzM protein of Streptococcus zooepidemicus and its application. Using genetic recombination technology, a murine monoclonal antibody with excellent efficacy in preventing or treating S. zooepidemicus infection was porcine-derived. The resulting chimeric antibody replaces the constant region of the murine antibody with that of the porcine antibody, thus avoiding the common inability of murine antibodies to effectively activate complement and Fc receptor-related immune effects in pigs. This antibody is highly effective in treating S. zooepidemicus infection in pigs, providing a new and effective biological product for the clinical prevention and treatment of S. zooepidemicus infection in pigs.