14 results about "Genetic recombination" patented technology

The invention provides a GMRM (Genome-based Mutant RNA Mapping) method taking a genome as a reference, in particular, the invention provides a method for cloning candidate functional genes of wheat stripe rust resistant plants and an analysis process, and the GMRM method disclosed by the invention can be used for simultaneously cloning two genes with complementary disease-resistant functions, namely Yr6NLR1 and Yr6NLR2. In an example of Yr6, the two disease-resistant genes are closely linked genetically, but the cloning process of the genes does not involve any genetic recombination. Therefore, theoretically, the method provided by the invention can be used for simultaneously cloning a plurality of functional complementary genes (namely genes participating in the same pathway) on the premise that the target gene can generate effective mutation through EMS mutagenesis.

The present invention provides a method for producing a culture strain that can be subcultured under conditions suitable for genetic recombination. (1) a culture step of culturing a plant piece to obtain a seedling; (2) a size confirmation step of confirming the size of the obtained seedlings; (3) The size of the seedling confirmed in the size confirmation step is (A) If the threshold is exceeded, the young plants are treated by removing dead leaves and roots, and the resulting culture is used for subsequent propagation. (B) If the concentration is below the threshold, the young plants are treated by leaving all healthy leaves and roots, and are used as culture lines for subsequent generations. a subculture preparation step; A method for producing a culture strain having the compound of formula (I) which is subcultured.

The invention is applicable to the field of gene engineering, and provides a mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle is formed by sequence fusion of mycobacterium tuberculosis Ag85B protein, TB8.4 protein and LS protein, and the amino acid sequence of the mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle is shown as SEQ ID NO: 2 in a sequence table. According to the invention, by virtue of a gene recombination technology, key immunogens Ag85B and TB8.4 of mycobacterium tuberculosis are combined with an LS protein fusion vector, and the Ag85B-TB8.4-LS protein nanoparticles with uniform particle size and stable structure are efficiently produced by virtue of an escherichia coli expression system. Animal experiment results show that the Ag85B-TB8.4-LS protein nanoparticle can simultaneously excite strong humoral immunity and cellular immunity response, not only brings a new idea for research and development of tuberculosis vaccines, but also can be popularized and applied to research and development of other infectious disease vaccines due to the modular design, and has important scientific significance and industrialization prospects.

The present application relates to the technical field of plant breeding, and particularly relates to a method for evaluating the double haploid induction potential of parent combinations in plant breeding. The present application constructs a method for evaluating the double haploid induction potential of parent combinations in plant breeding through genetic recombination simulation, a phenotype prediction model and a comprehensive evaluation function of multi-trait collaborative selection, can quantitatively predict the induction potential of different parent combinations before induction, can simultaneously consider multiple breeding objectives, realize collaborative optimization, and thus help to prioritize the allocation and scale planning of breeding resources. The present application can effectively save induction and identification resources, accelerate the breeding process of excellent varieties, and has a wide application prospect in the breeding of corn, rice, wheat and other plants.

The present invention provides a novel marker for genetic recombination in Starmerella bombicola and a method for using the same. The present invention also provides a Starmerella bombicola mutant strain in which the expression of an orotate phosphoribosyltransferase-like protein or a protein equivalent thereto is suppressed or inactivated, a uracil-requiring selection marker consisting of the gene, and a method for producing a Starmerella bombicola transformant using the selection marker.

The present application relates to the field of genetic engineering, in particular to a plant constitutive expression promoter and application thereof. The plant constitutive expression promoter provided by the present application is selected from the DNA sequence shown in any one of SEQ ID NO. 1-6. The plant constitutive expression promoter provided by the present application can regulate gene expression in plants after genetic recombination, and has significant value in practical application. Through genetic modification of crop varieties by the promoter, such as regulating the expression of target genes in plants by the promoter, ideal transgenic plant varieties can be cultivated.

The present invention provides: a novel marker for genetic recombination of Starmera bombicola; and a method for using the same. The present invention pertains to: a Starmerella bombicola mutant strain in which expression of an orotate phosphoribosyltransferase-like protein or an equivalent protein is suppressed or the protein is inactivated; a uracil auxotrophic selectable marker comprising the gene; and a method for producing a transformant of Starmerella bombicola using the selectable marker.

The invention relates to the technical field of plant breeding, in particular to a method for evaluating the double haploid induction potential of a parent combination in plant breeding. The method for evaluating the induction potential of the double haploids of the parent combination in plant breeding is constructed through a comprehensive evaluation function of genetic recombination simulation, a phenotype prediction model and multi-character collaborative selection, the induction potential of different parent combinations can be quantitatively predicted before induction, multiple breeding targets can be considered at the same time, collaborative optimization is achieved, and the method is suitable for large-scale popularization and application. Therefore, the priority allocation and scale planning of breeding resources are facilitated. The method can effectively save induction and identification resources, accelerates the breeding process of excellent varieties, and has wide application prospects in breeding of plants such as corn, rice and wheat.

A method for producing self-rejuvenating and safe cells, and a self-rejuvenating and safe repair cell are provided. [Solution] A technology and its use are provided for controlling the Jak-Stat pathway to differentiate, dedifferentiate, and rejuvenate cells. By quantitatively and / or periodically controlling gene or protein targets of the Jak-Stat signaling pathway in cells using combinations of small molecule compounds, cytokines, or recombinant proteins, gene editing technology, or genetic recombination technology, rejuvenated cell products and / or different types of cell products are obtained. This product can be applied to the reprogramming of cells, tissues, organs, and living organisms; the construction of tissue engineering materials; the repair of damaged and aging, degenerated tissues and organs in mammals; the delay or reversal of the progression of aging in cells, tissues, organs, and living organisms; and the immunomodulation of cells, tissues, organs, and living organisms.

The invention belongs to the technical field of biology, and particularly relates to a method for improving rice genetic recombination frequency. The HEI10 gene is over-expressed in rice, and a rice plant or a rice variety with improved genetic recombination frequency is obtained through screening. According to the invention, a scheme for improving the genetic recombination frequency is established in rice through a transgenosis method instead of a knockout method. Transgenic components of the gene can be screened and removed in offspring through genetic isolation, so that the genetic recombination frequency of the offspring is recovered to a normal level, and the stability of genome and maturing rate is facilitated. And the overexpression of the HEI10 basically does not influence the normal growth and the maturing rate, so that the method has practical significance.

The invention belongs to the technical field of gene engineering, and particularly relates to a gene OsMCS1 for regulating and controlling meiosis of rice as well as an encoding protein and application of the gene OsMCS1. The nucleotide sequence of the gene OsMCS1 is as shown in SEQ ID NO. 1. Through knockout of the gene OsMCS1 in the rice, the rice chromosome separation after gene knockout is abnormal, the embryo sac is partially aborted, the chromosome recombination frequency is improved by 1.5-2.0 times compared with that of a wild type and a knockout line, and the genetic recombination frequency is improved, so that the breeding efficiency is improved, and a new means is provided for improving the rice genetic breeding efficiency.

Provided are a novel marker for gene recombination of Starmerella bombicola and a method for using the same. The present invention provides an adenine auxotrophic selection marker consisting of a gene encoding a phosphoribosyl-glycinamide formyltransferase-like protein or a gene equivalent thereto. The present invention also provides a Starmerella bombicola mutant strain in which expression of a phosphoribosyl-glycinamide formyltransferase-like protein or a protein equivalent thereto is suppressed or the protein is inactivated.

The present disclosure provides compositions, methods, and systems related to genome editing technology. In particular, the present disclosure provides a novel CRISPR-based genome editing technology that involves the generation of abasic sites to facilitate genetic recombination, without the need for breaks in the DNA. The compositions, methods, and systems described herein address many of the drawbacks of currently available approaches, including off-target effects and cellular toxicity.