51 results about "Genetic recombination" patented technology

The invention relates to the technical field of data processing, and discloses a heterogeneous computing acceleration method and system based on a deep learning framework network. The method comprises the following steps: acquiring performance parameters of heterogeneous computing equipment to obtain an equipment characteristic data set; receiving a calculation task and analyzing the calculation task into an operation sequence; the operation sequence is coded into a gene sequence, task decomposition is optimized through a genetic recombination algorithm, and a subtask set marked with acceleration characteristics is obtained; performing matching analysis on the sub-task set and the equipment characteristic data set to obtain a task allocation scheme; deploying the subtasks to corresponding equipment according to the allocation scheme to obtain a distributed execution framework; and monitoring the running state of the framework in real time, and dynamically adjusting resource allocation to obtain a calculation result of speed-up ratio improvement. According to the method, a self-adaptive task decomposition and resource allocation and dynamic load balancing mechanism can be realized, and the execution efficiency and the resource utilization rate of the deep learning task are improved.

The invention relates to the technical field of a preparation method of a type A recombinant botulinum toxin, in particular to a type A botulinum toxin obtained by adopting a gene recombination technology, and the amino acid sequence of the type A botulinum toxin is consistent with the sequence of the type A botulinum toxin prepared by extracting and purifying a wild strain Clostridium botulinum. According to the technical scheme, the single-chain protein of the botulinum toxin A sequentially comprises a botulinum toxin A light chain or a modified botulinum toxin A light chain, a connecting region and a botulinum toxin A heavy chain. According to the scheme, the high-quality recombinant botulinum toxin A is obtained after the single-chain protein is subjected to fermentation, crude purification, enzyme digestion and fine purification. According to the technical scheme, the problems of complex process, long period, high safety risk and the like in the existing process for preparing the botulinum toxin A from the wild bacterial strain clostridium botulinum can be solved, and meanwhile, the problem that the amino acid sequence consistent with that of the botulinum toxin A prepared from the wild bacterial strain clostridium botulinum cannot be obtained through the existing gene recombination technology is solved.

The invention relates to recombinant saccharomyces cerevisiae for de novo synthesis of mevalonic acid as well as a construction method and application of the recombinant saccharomyces cerevisiae, and belongs to the technical field of genetically engineered bacteria. The technical problem to be solved by the invention is to provide the recombinant saccharomyces cerevisiae for de novo synthesis of mevalonic acid. According to the recombinant saccharomyces cerevisiae, saccharomyces cerevisiae is used as a chassis strain, and ACL, CTP1, RtME and MDH3 are over-expressed. A series of recombinant saccharomyces cerevisiae strains with high yield of mevalonic acid are constructed through a gene recombination method, the strains can be fermented in a conventional culture medium to prepare mevalonic acid, the fermentation process is simple, the cost is low, the yield is high, and the maximum yield of mevalonic acid can reach 5692.45 mg / L. According to the invention, the recombinant saccharomyces cerevisiae strain is used as a chassis strain, and recombinant genetically engineered bacteria for producing the santalene, the santalol and the patchouli alcohol are respectively obtained through gene recombination, so that the yields of the santalene, the santalol and the patchouli alcohol produced by fermentation are improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker-based rice disease resistance character whole genome association analysis method, which relates to the technical field of biology, and is characterized by comprising the following steps: selecting a representative genetic recombination population, carrying out high-density SNP genetic typing on the population, and combining recombination event positioning and linkage analysis to obtain the disease resistance character whole genome association analysis of rice. Obtaining genetic parameters reflecting recombination frequencies of different genome segments; on the basis of the obtained original genetic data, parent and offspring genotype information is further integrated, fine positioning and statistics are carried out on recombination breaking points, and a high-resolution genetic map is constructed. By constructing a high-resolution genetic map and combining feature engineering and machine learning, accurate identification and dynamic SNP encryption of a high-recombination region are realized, the marker coverage and associated signal capture capability of the region is enhanced, the functional site leak detection risk is reduced, and the detection efficiency is improved. The positioning accuracy and the molecular breeding application effect of the rice disease resistance character related candidate gene are improved.

The invention discloses a recombinant strain for efficiently expressing xanthine oxidase as well as preparation and application of the recombinant strain, and belongs to the technical field of enzyme engineering. According to the recombinant strain, four kinds of recombinant Pseudomonas putida strains are designed, and the strains can be used for efficiently expressing xanthine oxidase. The recombinant strain has the advantages of high expression quantity, simple affinity purification process flow, high purification yield and low production cost, and the recombinant xanthine oxidase has the specific activity similar to that of xanthine oxidase sold in the market at present and is suitable for various applications. The recombinant strain can realize high-efficiency expression of xanthine oxidase, and high-efficiency expression of xanthine oxidase is realized in P.putida through a gene recombination technology; the expressions are induced by adding an inducer, so that the maximum expression quantity of the xanthine oxidase is realized.

The invention provides a GMRM (Genome-based Mutant RNA Mapping) method taking a genome as a reference, in particular, the invention provides a method for cloning candidate functional genes of wheat stripe rust resistant plants and an analysis process, and the GMRM method disclosed by the invention can be used for simultaneously cloning two genes with complementary disease-resistant functions, namely Yr6NLR1 and Yr6NLR2. In an example of Yr6, the two disease-resistant genes are closely linked genetically, but the cloning process of the genes does not involve any genetic recombination. Therefore, theoretically, the method provided by the invention can be used for simultaneously cloning a plurality of functional complementary genes (namely genes participating in the same pathway) on the premise that the target gene can generate effective mutation through EMS mutagenesis.

The present invention provides a method for producing a culture strain that can be subcultured under conditions suitable for genetic recombination. (1) a culture step of culturing a plant piece to obtain a seedling; (2) a size confirmation step of confirming the size of the obtained seedlings; (3) The size of the seedling confirmed in the size confirmation step is (A) If the threshold is exceeded, the young plants are treated by removing dead leaves and roots, and the resulting culture is used for subsequent propagation. (B) If the concentration is below the threshold, the young plants are treated by leaving all healthy leaves and roots, and are used as culture lines for subsequent generations. a subculture preparation step; A method for producing a culture strain having the compound of formula (I) which is subcultured.

The invention provides genetically engineered saccharomycetes capable of simultaneously producing baicalein and baicalin as well as a construction method and application of the genetically engineered saccharomycetes. According to the invention, by weakening or deleting a glucose 5-phosphate dehydrogenase gene (ZWF1) and phenylpyruvate decarboxylase genes ARO10 and PDC5 in a saccharomyces cerevisiae genome and enhancing the expression of a pre-benzoic acid dehydratase gene Pha2, the metabolic flow of a shikimic acid pathway is increased, the aldrin pathway is blocked, and the yield of the shikimic acid pathway is increased. Therefore, the yield of L-phenylalanine serving as a starting material for synthesizing baicalein and baicalin is increased; meanwhile, exogenous genes PAL, 4CL, CHS, CHI, FNSII, F6H, CPR and F7GAT are integrated onto the genome by virtue of a gene recombination technology, so that baicalein and baicalin are produced from the beginning by taking glucose and glycerol as a mixed carbon source without adding precursor substances such as exogenous L-phenylalanine or malonyl CoA and the like.

The present invention provides a novel marker for genetic recombination in Starmerella bombicola and a method for using the same. An adenine-requiring selection marker consisting of a gene encoding a phosphoribosylglycine amidoformyltransferase-like protein or a gene equivalent thereto. A Starmerella bombicola mutant strain in which the expression of a phosphoribosylglycine amidoformyltransferase-like protein or a protein equivalent thereto is suppressed or inactivated.

The invention discloses a Trichoderma reesei engineering strain for producing trans-aconitic acid as well as a construction method and application of the Trichoderma reesei engineering strain, and relates to the technical field of biological engineering. The construction method comprises the following steps: transforming a coding gene of mitochondrial tricarboxylic acid transport protein and a coding gene of aconitic acid isomerase into a trichoderma reesei host bacterium, and carrying out gene recombination construction to obtain the trichoderma reesei engineering strain, the amino acid sequence of the mitochondrial tricarboxylic acid transporter is as shown in SEQ ID NO. 1; the amino acid sequence of the aconitic acid isomerase is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. A new production strain is provided for microbial fermentation production of trans-aconitic acid, a large amount of trans-aconitic acid can be produced through direct fermentation by using common carbon sources such as glucose or cellulose, and the highest yield can reach 26.5 g / L.

The present invention discloses a genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid by taking glucose as a substrate and applications thereof, and belongs to the technical field of genetic recombination and metabolic engineering. The genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid (MA) by taking glucose as the substrate disclosed in the present invention is modified with chassis microbes, and includes recombinant Corynebacterium glutamicum for a cis,cis-muconic acid pathway construction module and an intermediate high-yield module. Production capacity of strains is greatly improved; MA of 90.2 g / L is finally obtained in fermentation liquor; and possibilities are provided for green and low-cost production of numerous chemicals such as adipic acid and nylon-66.

The invention is applicable to the field of gene engineering, and provides a mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle, a preparation method and application thereof, the mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle is formed by sequence fusion of mycobacterium tuberculosis Ag85B protein, TB8.4 protein and LS protein, and the amino acid sequence of the mycobacterium tuberculosis Ag85B-TB8.4-LS protein nanoparticle is shown as SEQ ID NO: 2 in a sequence table. According to the invention, by virtue of a gene recombination technology, key immunogens Ag85B and TB8.4 of mycobacterium tuberculosis are combined with an LS protein fusion vector, and the Ag85B-TB8.4-LS protein nanoparticles with uniform particle size and stable structure are efficiently produced by virtue of an escherichia coli expression system. Animal experiment results show that the Ag85B-TB8.4-LS protein nanoparticle can simultaneously excite strong humoral immunity and cellular immunity response, not only brings a new idea for research and development of tuberculosis vaccines, but also can be popularized and applied to research and development of other infectious disease vaccines due to the modular design, and has important scientific significance and industrialization prospects.

The invention discloses a preparation method of a gamma S-crystalin protein antigen and a preparation method of an egg yolk antibody of the gamma S-crystalin protein antigen. The preparation method comprises the following steps: preparing the gamma S-crystalin protein antigen by adopting a gene recombination method, and emulsifying the gamma S-crystalin protein antigen; healthy laying hens are selected for injection immunization; an egg yolk antibody IgY is extracted from an egg obtained from an immunized laying hen, and an enzyme-linked immunosorbent assay method for detecting gamma S-crystalin in a sample is established at the same time. An expression plasmid containing gamma S-crystalin protein is constructed and expressed in BL21 (DE3) escherichia coli, an expression product obtained after separation and purification serves as an antigen and is mixed with a Freund's adjuvant to be emulsified, then laying hens are immunized, eggs are collected, the egg yolk antibody IgY in egg yolk is extracted, and the antibody is natural immune globulin. According to the invention, a polyclonal antibody technology is combined with an ELISA method, and the prepared anti-egg yolk antibody IgY can be used for efficiently detecting the existence of gamma S-crystalin protein. The method is suitable for preparing the gamma S-crystalin egg yolk antibody IgY, and the obtained antibody is safe and high in titer and has good development and application prospects.

The present application relates to the technical field of plant breeding, and particularly relates to a method for evaluating the double haploid induction potential of parent combinations in plant breeding. The present application constructs a method for evaluating the double haploid induction potential of parent combinations in plant breeding through genetic recombination simulation, a phenotype prediction model and a comprehensive evaluation function of multi-trait collaborative selection, can quantitatively predict the induction potential of different parent combinations before induction, can simultaneously consider multiple breeding objectives, realize collaborative optimization, and thus help to prioritize the allocation and scale planning of breeding resources. The present application can effectively save induction and identification resources, accelerate the breeding process of excellent varieties, and has a wide application prospect in the breeding of corn, rice, wheat and other plants.

The present application relates to the technical field of plant breeding, and particularly relates to a genetic background evaluation method of backcross improvement population. The present application divides chromosome intervals and molecular markers based on plant genetic recombination exchange rules, obtains typing results of the simplified markers, calculates background recovery rate of the backcross improvement population based on the typing results, combines the background recovery rate with the structure of the recovered fragments to evaluate the genetic background recovery degree of the backcross improvement population, and selects effective single plants with small genetic differences from the receptor parent and relatively concentrated unrecovered segments for pollination and protection, thereby greatly reducing field workload and accelerating the backcross breeding process.

The present invention discloses a method for promoting chondrocyte growth, comprising the following steps: (1) constructing BMP-2 gene recombinant MSC (MSCdBMP2) cells to express BMP-2 protein on the surface of MSCdBMP2 cells; (2) preparing exosomes (MSCdBMP2Exo) derived from MSCdBMP2; (3) stimulating MSCdBMP2 cells and MSCdBMP2Exo to significantly promote chondrocyte growth. The present invention clones the BMP-2 gene into MSC cells through genetic recombination technology to prepare new BMP-2 gene recombinant MSC (MSCdBMP2) cells; at the same time, the exosomes (MSCdBMP2Exo) prepared from MSCdBMP2 cells have a biological function similar to that of MSCdBMP2 cells in promoting chondrocyte growth.

To create a strain of 5-aminolevulinic acid-producing bacteria, which is a strain suitable for the industrial production of 5-aminolevulinic acid from the viewpoint of safety, by using a technique that does not leave foreign genes in the final strain, particularly self-cloning technology although it is a genetic recombination technique; and to provide a method for producing 5-aminolevulinic acid using the created strain.SOLUTION: The above problem is solved by: creating a strain of photosynthetic bacteria belonging to α-proteobacteria class, which has an expression control region including PrrnB promoter upstream of a gene (hemT) encoding HemT, a 5-aminolevulinic acid synthase, the expression control region being derived from the same species of photosynthetic bacteria as the strain and inducing the expression of hemT, and the induction resulting in an increase in the productivity of 5-aminolevulinic acid compared to a wild-type strain; and culturing the created strain.SELECTED DRAWING: None

The invention relates to the technical field of material chemical industry, in particular to a method and a system for extracting natural pigment for coating based on microbial fermentation, which comprises the following steps: acquiring a plurality of initial strains, acquiring a genetic recombination strain group, preparing single-cell suspension from the genetic recombination strains, performing fluorescence detection on the single-cell suspension, and confirming an optimal mutant strain. The method comprises the following steps: obtaining a mutant strain pigment crude extract, confirming a maximum absorption wavelength, culturing an optimal mutant strain, respectively carrying out absorbance determination on each mutant strain experimental group to obtain an absorbance value set, confirming an optimal fermentation temperature, carrying out large-scale fermentation to obtain a high-secretion fermentation liquid, carrying out wall breaking treatment to obtain a wall-broken thallus suspension, and carrying out freeze drying to obtain the high-secretion thallus suspension. The method comprises the following steps: preparing an intracellular pigment solution, carrying out microencapsulation on the intracellular pigment solution to obtain pigment microspheres, extracting a verification pigment extracting solution from the pigment microspheres, and obtaining the natural pigment by utilizing the verification pigment extracting solution. The problems of low extraction efficiency, high energy consumption and environmental pollution during extraction of the natural pigment for the coating can be solved.

To provide a technique to circumvent the adverse effects that arise during treatment based on CAR T-cell therapy.SOLUTION: Disclosed herein are methods of manufacturing through genetic recombination a bi-specific T-cell that expresses a chimeric antigen receptor for promoting the in vivo expansion and activation of effector cells and a second chimeric antigen receptor or TcR specific for a ligand on a tumor. Methods of administering bi-specific chimeric antigen receptor-bearing cells to subjects in need thereof are also provided.SELECTED DRAWING: Figure 1

To identify a dormant gene cluster of a filamentous fungus, and search a new compound derived from the dormant gene by using the identification.SOLUTION: Provided is a genome rearrangement method of a filamentous fungus which includes a genome rearrangement step of expressing a heat-resistant restriction enzyme derived from a thermophile in a filamentous fungus cell and generating random genetic recombination in a genome inside a cell by the restriction enzyme, where the restriction enzyme is expressed under the control of a homeostatic promoter in the temperature condition of approximately 28°C to approximately 37°C. Also provided is a production method of a compound (secondary metabolite) derived from a dormant gene in a filamentous fungus by utilizing the genome rearrangement method.SELECTED DRAWING: None

This invention relates to the field of underwater adhesive materials, and particularly to an injectable self-healing underwater protein and its uses. The invention provides a fusion protein comprising a mussel byssal protein fragment and a coiled-helical structure fragment. This invention involves genetic recombination of mussel byssal protein and the coiled-helical structure, thereby providing a novel fusion protein that can self-assemble in solution to form a gel material, possessing both the interfacial adhesiveness of mussel byssal protein and the intrinsic adhesiveness of the coiled-helical structure, thus achieving super-strong underwater adhesion.

The present invention provides a novel marker for genetic recombination in Starmerella bombicola and a method for using the same. The present invention also provides a Starmerella bombicola mutant strain in which the expression of an orotate phosphoribosyltransferase-like protein or a protein equivalent thereto is suppressed or inactivated, a uracil-requiring selection marker consisting of the gene, and a method for producing a Starmerella bombicola transformant using the selection marker.

The present application relates to the field of data processing technology, and discloses a heterogeneous computing acceleration method and system based on a deep learning framework network. The method includes: collecting performance parameters of heterogeneous computing devices to obtain a device characteristic data set; receiving computing tasks and parsing them into operation sequences; encoding the operation sequences into gene sequences, optimizing task decomposition through a genetic recombination algorithm, and obtaining a subtask set marked with acceleration characteristics; matching and analyzing the subtask set with the device characteristic data set to obtain a task allocation scheme; deploying the subtasks to the corresponding devices according to the allocation scheme to obtain a distributed execution framework; monitoring the framework operation status in real time, dynamically adjusting resource allocation, and obtaining a calculation result with an improved acceleration ratio. The present application can realize adaptive task decomposition and resource allocation and dynamic load balancing mechanism, thereby improving the execution efficiency and resource utilization of deep learning tasks.

A genetically recombinant microorganism, according to the idea of the present disclosure, comprises at least one foreign gene, wherein the foreign gene comprises a LvaED gene derived from a strain selected from the genus Pseudomonas, and the LvaED gene is introduced into a microorganism selected from the genus Ralstonia.

The present invention relates to the field of genetic engineering and synthetic biology, more particularly to means and methods for promoting genetic recombination. The present application discloses novel Cre recombinase dependent recombination sites that support methods of simultaneous cloning and testing.

Provided are a novel genetic recombination marker of Starmerella bombicola, and a method for using the same. An adenine-requirement selectable marker comprising a gene encoding a phosphoribosylglycinamide formyltransferase-like protein or an equivalent gene. A mutant strain of Starmerella bombicola in wich the expression of a phosphoribosylglycinamide formyltransferase-like protein or an equivalent protein is suppressed or inactivated.

The present invention relates to the technical fields of fermentation engineering and genetic engineering, and particularly relates to a method for synthesizing lactic acid monomer using sucrose as a raw material. Through the genetic recombination technology of site-directed gene replacement, the promoter sequence of the sucrose metabolic operon in the production strain is corrected, enabling it to maintain a high level of expression throughout the production process of lactic acid monomer, demonstrating an obvious ability to highly catabolize sucrose to synthesize lactic acid monomers with high optical purity and high chemical purity (D-lactic acid or L-lactic acid). For example, strains DSAPW5 and LSAPW5 are fermented for 28-30 h under the condition of 25-50 °C, and the fermentation levels of D-lactic acid and L-lactic acid can reach 158.58 g / L and 159.65 g / L respectively, and the optical purity can reach 99.94% and 99.95%. The present invention can be applied to the industrial production of lactic acid monomer using sucrose or sucrose-containing raw materials.

The invention relates to the technical field of plant breeding, in particular to a genetic background evaluation method of a backcross improved population. The method comprises the following steps: dividing chromosome intervals and molecular markers based on a plant genetic recombination exchange rule to obtain a typing result of a simplified marker, calculating a background recovery rate of a backcross improved population based on the typing result, and combining the background recovery rate with a recovery fragment structure to evaluate the genetic background recovery degree of the backcross improved population. Effective single plants which are small in genetic difference with receptor parents and relatively concentrated in non-recovery sections are screened for pollination and protection, so that the field workload is greatly reduced, and the backcross breeding process is accelerated.

The purpose of the present invention is to provide a set of two polypeptides for use in light-dependent genetic recombination in which the N-terminal side fragment and the C-terminal side fragment of a Cre protein having an amino acid sequence of SEQ ID NO: 1 respectively bind to two proteins light-dependently forming a dimer.

The present application relates to the field of genetic engineering, in particular to a plant constitutive expression promoter and application thereof. The plant constitutive expression promoter provided by the present application is selected from the DNA sequence shown in any one of SEQ ID NO. 1-6. The plant constitutive expression promoter provided by the present application can regulate gene expression in plants after genetic recombination, and has significant value in practical application. Through genetic modification of crop varieties by the promoter, such as regulating the expression of target genes in plants by the promoter, ideal transgenic plant varieties can be cultivated.