40 results about "Mycoplasma" patented technology

The invention relates to the technical field of sewage purification, and particularly discloses a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The invention relates to a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The method comprises the following steps: S1, collecting, adjusting and precipitating sewage through a pretreatment unit, and intercepting solid garbage at the same time; s2, introducing the pretreated sewage into a fish-fungus-algae symbiotic unit, putting fungi and algae into the unit, and stocking herbivorous fishes; s3, effluent of the fish-fungus-algae symbiosis unit is guided into a shellfish purification unit, and shellfish is put into the shellfish purification unit; s4, leading effluent of the shellfish purification unit into an aquatic plant purification unit, and planting aquatic plants in the aquatic plant purification unit. The pollution reduction type water quality purification method combining the shellfish and grass carp bacteria and algae can be used for scenes such as village and town domestic sewage, agricultural non-point source pollution and sewage treatment plant tail water, and has the advantages of pollutant reduction and water body self-purification capability reconstruction.

The invention relates to the technical field of biology, in particular to a primer probe combination product for respiratory tract infection detection. A plurality of specific primers and self-quenching probes are integrated in a single reaction system; simultaneous, rapid and rapid detection on 15 respiratory pathogens (including influenza A virus, influenza B virus, respiratory syncytial virus, severe acute respiratory syndrome coronavirus 2, mycoplasma pneumoniae, parainfluenza virus, rhinovirus, enterovirus, coronavirus, adenovirus, metapneumovirus, bordetella pertussis, bocavirus, chlamydia pneumoniae and chlamydia psittaci) is realized. The detection is accurate.

The invention provides a nano antibody specifically bound with mycoplasma pneumoniae P1 protein or an antigen binding fragment thereof, which can specifically recognize and bind the mycoplasma pneumoniae P1 protein and has better affinity with the mycoplasma pneumoniae P1 protein. The mycoplasma pneumoniae P1 protein can be used for preparing products for diagnosing, preventing and / or treating mycoplasma pneumoniae P1 protein infection or diseases caused by the mycoplasma pneumoniae P1 protein infection, or detecting the existence or the level of the mycoplasma pneumoniae P1 protein in a sample.

The invention discloses a reagent combination and a kit for detecting four respiratory tract pathogens based on qPCR, and belongs to the technical field of microbiological detection. Wherein the four respiratory tract pathogens are respectively human adenovirus, human bocavirus, bordetella pertussis and mycoplasma pneumoniae; the kit also comprises an optimized PCR (Polymerase Chain Reaction) buffer solution, DNA (Deoxyribose Nucleic Acid) polymerase and UDG enzyme. Through screening processes such as specific primer probe design, reagent formula and reaction system optimization, high sensitivity and high specificity are maintained while rapid detection is performed, and missing detection and misjudgment are effectively avoided.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The utility model relates to an immunological detection kit for mycoplasma pneumoniae in the technical field of immunological detection. The immunological detection kit comprises a main shell, an auxiliary shell is connected to the bottom of the main shell in an embedded mode. A clamping block is fixedly connected to the interior of the main shell; the clamping blocks are symmetrically arranged in the main shell; the supporting plate is placed through a buckling structure of the multiple clamping blocks. A sample pad is arranged at the top of the supporting plate; the sample pad is arranged between the main shell and the supporting plate; the top of the supporting plate is provided with a conjugate dilution pad; the conjugate dilution pad is arranged on one side of the sample pad; the top of the supporting plate is provided with an NC membrane, the NC membrane is arranged on one side of the conjugate dilution pad, the top of the supporting plate is provided with an absorption pad, and the absorption pad is arranged on one side of the NC membrane. And antibodies generated by mycoplasma pneumoniae infection and antibodies caused by other pathogen infection can be accurately distinguished.

This invention relates to a primer and probe composition and kit for detecting Mycoplasma pneumoniae and its drug resistance gene mutations. The primer and probe composition includes: a primer and probe set for detecting the wild type of Mycoplasma pneumoniae; a primer and probe set for detecting the A2063G mutation in Mycoplasma pneumoniae; a probe for detecting the A2064G mutation in Mycoplasma pneumoniae; a primer and probe set for detecting a human gene internal standard; and a competitive blocking agent. The primer and probe composition of this invention can detect Mycoplasma pneumoniae and its A2063G and A2064G drug resistance mutation sites with high sensitivity and specificity, solving the technical problems of false positives and insufficient specificity in existing quantitative real-time PCR for detecting single-base mutations in Mycoplasma pneumoniae.

The invention relates to the technical field of probiotic application, in particular to application of lactobacillus crispatus HC1261 in preparation of a microbial agent for improving female reproductive health and a composition for improving vagina inflammation. The lactobacillus crispatus HC1261 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 16, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.27355; the microbial inoculum for improving female reproductive health can reduce the load of the ureaplasma urealyticum in the vagina. The lactobacillus crispatus HC1261 can be used for pertinently reducing the load of the ureaplasma urealyticum in the vagina, and can be used for reducing the related health risks of urethritis, cervicitis, pelvic inflammation, infertility and the like caused by the ureaplasma urealyticum.

The invention discloses a detection method for simultaneously determining multiple respiratory pathogens. According to the present invention, with the help of the recognized excellent signal amplification tool-CRISPR / Cas12a system and the DNA nanometer machine, the metal element labeling and the ICP-MS detection technology are assisted, such that the simultaneous detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the influenza A virus (H1N1) and the mycoplasma pneumoniae (MP) in the respiratory pathogens is completed so as to easily maintain the respiratory system safety of the whole human. When a compound formed by Cas12a / crRNA is specifically combined with a corresponding target DNA, the trans-shear activity of a CRISPR / Cas12a system can be activated, so that corresponding DNAzyme can be sheared, a DNA nano machine cannot run, lanthanide series metal elements on the DNA nano machine cannot be released, ICP-MS outputs a low signal, and quantitative analysis of multiple DNAs can be realized. The method for detecting the respiratory pathogens can realize simultaneous detection of multiple components, reduces the detection cost and the sample consumption, and has the advantages of high sensitivity and good specificity.

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition for detecting pathogens in a female lower genital tract, a kit and application. The oligonucleotide composition disclosed by the invention can be used for rapidly and simultaneously detecting a plurality of pathogens in gardnerella vaginalis, atriurella vaginalis, prevotella vulgaris, campylobacter mobilis, bacteroides, trichomonas vaginalis and mycoplasma in a sample in a high-specificity manner, and has high sensitivity and high stability; and a rapid, non-invasive, accurate and convenient detection method is provided for female reproductive health detection.

The application discloses a detection system of mycoplasma pneumoniae. According to the P1 gene of mycoplasma pneumoniae, RPA primers and crRNA required by a CRISPR system with LbCas12a as a core enzyme are designed and screened, an RPA system and a CRISPR system are established, the influence of each component of the CRISPR-LbCas12a system on the RPA system reaction and the influence of each component of the RPA system on each component of the CRISPR-LbCas12a system are tested, components are optimized according to the test results, and an RPA-LbCas12a integrated system capable of detecting a conserved fragment on the P1 gene of mycoplasma pneumoniae is established. The RPA-LbCas12a integrated system established in the application has high sensitivity, high detection efficiency, reduces nucleic acid pollution, and has a wide application prospect.

The invention belongs to the technical field of research and development of quintuplet vaccines, and particularly relates to a chicken Newcastle disease virus strain LaSota, a chicken Newcastle disease virus strain H120, a chicken Newcastle disease virus strain N49, a chicken Newcastle disease virus strain FADV-4 and a chicken Newcastle disease virus strain HN01. The chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain FADV-4 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain FADV-4 and the chicken Newcastle disease virus strain HN01 are combined. The chicken Newcastle disease virus LaSota strain is proliferated in chicken embryo fibroblasts or chicken kidney cells, and the chicken Newcastle disease virus LaSota strain and the preparation method thereof have the beneficial effects that the chicken Newcastle disease virus LaSota strain and the preparation method thereof can be used for preparing the chicken Newcastle disease virus LaSota strain. The preparation method comprises the following steps: selecting an inactivated newcastle disease virus LaSota strain, an inactivated bronchitis H120 strain, an inactivated bursal disease N49 strain, an inactivated fowl adenovirus FADV-4 strain and an inactivated mycoplasma synovium HN01 strain; it can be guaranteed that a follow-up vaccine has the advantages of being low in toxicity, high in biological safety, high in cross protection capacity, high in immune response speed, long in antibody protection period, high in cellular immune level and high in virus culture content.

The invention discloses a Mycomonas rosae CCSM007 strain separated from human skin and capable of improving skin health and a metagen of the Mycomonas rosae CCSM007 strain, and belongs to the field of microbial technologies and medicines. The myxomonas rosae CCSM007 provided by the invention has a good barrier function repairing effect and application prospect in the aspect of external use, and the application prospect is specifically shown as follows: the activity of damaged HaCaT cells is improved in vitro, the expression of gene levels and protein levels in the aspects of functional proteins and structural proteins after damage is improved, and meanwhile, the expression of sphingomyelinase 1 (SMPD1) is improved. The metaplast of the myxomonas rosae has a huge application prospect in preparation of cosmetics or medicines for repairing skin barriers.

This invention discloses a multiplex qPCR kit for the simultaneous detection of six respiratory pathogens. The multiplex qPCR kit of this invention can simultaneously detect six common respiratory pathogens: adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, and chlamydia pneumoniae. It has the advantages of high specificity, high sensitivity, good repeatability, and is unaffected by interfering substances. Furthermore, the kit is simple to operate and quick to use for detecting adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, or chlamydia pneumoniae. It can simultaneously detect these six different common respiratory pathogens using a two-tube reaction system, and the detection results are accurate. It is suitable for rapid clinical detection of respiratory pathogens, which helps in timely and accurate treatment of the infecting pathogens.

A kit of detecting Mycoplasma pneumoniae is provided, and the kit comprises a combination of primers and probe consisting of a forward primer, a reverse primer and a probe. The forward primer is selected from the group consisting of SEQ ID NO: 2-3 and complementary sequences thereof, the reverse primer is selected from the group consisting of SEQ ID NO: 6-8 and complementary sequences thereof, and the probe is SEQ ID NO: 9 or a complementary sequence thereof. A method for detecting Mycoplasma pneumoniae using the above kit is also provided.

The invention relates to the technical field of biological detection, and discloses a composition for detecting respiratory tract infection pathogens, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting legionella pneumophila, bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, mycobacterium tuberculosis, aspergillus and cryptococcus neoformans in a sample with high specificity, and has relatively high sensitivity and stability. Compared with traditional detection methods such as pathogen isolated culture and genome sequencing, the efficiency of identifying and jointly detecting multiple respiratory tract pathogens by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, the AUC is 89.8%, and the evaluation model is used for evaluating the quality of polygonatum sibiricum. The method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker primer pair related to swine tolerance mycoplasma pneumonia traits in an HSPA12A gene coding region and application of the SNP marker primer pair. The locus of the SNP marker is a molecular marker of the rs3472181281 nucleotide locus of a pig HSPA12A gene coding region in a reference sequence of an international pig genome version 11.1, and the SNP marker has G / C polymorphism. According to a primer pair for detecting the SNP marker, an upstream primer is SEQ ID NO: 2, and a downstream primer is SEQ ID NO: 3. The SNP marker provided by the invention can be applied to marker-assisted selection of swine mycoplasmal pneumonia resistant traits, and swine populations or strains resistant to swine mycoplasmal pneumonia traits are screened by identifying the genotype of the SNP marker. Establishment of the group or strain can improve the capability of tolerating swine mycoplasmal pneumonia and produce more social and economic benefits.

The invention belongs to the technical field of biological detection, and particularly relates to a fluorescence-quenching labeled double-chain universal probe and a mediated amplification method and application thereof. The nucleic acid detection system comprises a fluorescence-quenching labeled double-stranded universal probe, the double-stranded universal probe is composed of two complementary oligonucleotide chains, and the nucleotide sequences of the double-stranded universal probe are shown as SEQ ID NO: 1-4. According to the pathogen nucleic acid detection method based on the universal probe and the target specific anchoring primer, a real-time quantitative PCR or isothermal amplification technology and a fluorescence resonance energy transfer mechanism are integrated, and synchronous and accurate detection of mycoplasma pneumoniae, streptococcus pneumoniae, haemophilus influenzae, influenza A virus, influenza B virus, novel coronavirus and the like is achieved. The method does not need pathogen culture, solves the problems of insufficient specificity, high cost, tedious operation and the like of a traditional detection technology, and can be applied to rapid diagnosis of clinical pathogen infection, reasonable use guidance of antibiotics and epidemiological monitoring.

The present application relates to a SNP marker primer pair related to a pig mycoplasma pneumonia tolerance trait on pig chromosome 15 and application thereof. The SNP marker site is a molecular marker of nucleotide site rs1110963643 on pig chromosome 15 in the international pig genome 11.1 version reference sequence, and has T / C polymorphism. A primer pair for detecting the SNP marker, the upstream primer is SEQ ID NO: 2, and the downstream primer is SEQ ID NO: 3. The SNP marker provided by the present application can be applied to marker-assisted selection of pig mycoplasma pneumonia tolerance traits, and the pig population or strain with tolerance to pig mycoplasma pneumonia is screened by identifying the genotype of the SNP marker. The establishment of the population or strain can improve the ability to tolerate pig mycoplasma pneumonia, and produce more social and economic benefits.

The present invention pertains to a method for detecting microorganisms such as mycoplasma or mouse minute virus (MMV) in a sample, the method comprising: (i) performing PCR on a sample suspected of comprising mycoplasma DNA with a PCR reaction mixture comprising (1) a first primer (Pl) annealing to the reverse complement of a nucleic acid sequence of SEQ ID NO: 1 and a second primer (P2) annealing to a nucleic acid sequence of SEQ ID NO: 2, or (2) a third primer (P3) annealing to the reverse complement of a nucleic acid sequence of SEQ ID NO: 109 and a fourth primer (P4) annealing to a nucleic acid sequence of SEQ ID NO: 110; and (ii) detecting a PCR product, wherein the detection of a PCR product indicates that the sample comprises microroganisms, and the absence of a PCR product indicates that the sample does not comprise microroganisms. The present invention further pertains to a pair of primers for detecting mycoplasma or virus in a sample, and to a kit for detecting mycoplasma or virus in a sample.

The invention provides a targeted intestinal flora small molecule medicine and application thereof in treatment of type II diabetes mellitus, the medicine is prepared from traditional Chinese medicine active ingredients, chemical active ingredients, bioactive peptides and active probiotics according to a specific proportion, the chemical effective component is a metformin hydrochloride and prebiotic compound, the bioactive peptide comprises gamma-glutamyl peptide and ovalbumin hypoglycemic peptide, and the active probiotic is freeze-dried powder of ackermania muciniphila with high activity. The preparation method comprises the steps of pretreatment, step-by-step mixing, granulation and drying, can regulate the balance of intestinal flora, improve insulin resistance and repair the intestinal barrier function, and can be used for treating or preventing type II diabetes mellitus.

The invention belongs to the field of biomedical treatment, and particularly relates to a convective amplification primer pair and probe for respiratory tract pathogenic bacteria and multiple detection application of the convective amplification primer pair and probe. The convection amplification primer pair comprises a legionella pneumophila primer pair, a mycoplasma pneumoniae primer pair and a bordetella pertussis primer pair, and the legionella pneumophila primer pair, the mycoplasma pneumoniae primer pair and the bordetella pertussis primer pair are respectively composed of an upstream primer and a downstream primer; each of the upstream primer and the downstream primer comprises a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid complementary sequence and is not complementary with target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary with the interface sequence, so that the primer sequence has a curved hairpin structure, a probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.

The invention provides a mycoplasma pneumoniae / chlamydia combined detection method, which efficiently enriches target genes through cascade reaction of RPA (recombinase polymerase amplification) pre-amplification (37 DEG C + / -1 DEG C, 5 minutes) and LAMP (loop-mediated isothermal amplification) targeted amplification (40 DEG C + / -2 DEG C, 15 minutes). A dUTP / UDG anti-pollution system and a closed tube operation process are adopted, an amplification product is directly mixed with a double-labeled colloidal gold probe, and double pathogens are synchronously detected through an immunochromatography test strip. The test strip is provided with two independent detection lines, FAM and digoxin reporter molecules are captured respectively, detection is completed within 25 minutes, sensitivity is high, cross contamination is avoided, and the test strip is suitable for rapid diagnosis of basic respiratory pathogens.

The invention provides an application of tripterygium wilfordii in preparation of a product for preventing and / or treating poultry infectious serositis and / or mycoplasma synoviae diseases or complications caused by infection of pathogenic bacteria of the poultry infectious serositis and / or the mycoplasma synoviae diseases. The tripterygium wilfordii extract can be used for preventing and / or treating poultry infectious serositis and / or mycoplasma synoviae diseases or complications caused by infection of pathogenic bacteria of the mycoplasma synoviae diseases. The inventor finds that the tripterygium wilfordii or the tripterygium wilfordii extract has a good effect in prevention and treatment of the poultry infectious serositis and the poultry inflammatory diseases through a large number of screening, and further provides application of the tripterygium wilfordii or the tripterygium wilfordii extract in preparation of the medicine for preventing and / or treating the poultry infectious serositis and the poultry inflammatory diseases.

The invention relates to a preparation method of a mycoplasma pneumoniae natural antigen, which belongs to the technical field of biological detection, and comprises the following steps: (1) concentrating 10L of mycoplasma pneumoniae culture solution to 100mL by using a hollow fiber column, performing liquid replacement by using the hollow fiber column after concentration, adding 4000 mL of replacement liquid in each replacement, and repeating the replacement for three times; (2) pretreatment of thalli; (3) ultrasonic treatment; and (4) adding the liquid obtained in the step (3) into isometric absolute ethyl alcohol, placing in a water bath kettle at 95 DEG C, and refluxing for 1h. The mycoplasma pneumoniae antigen with high purity, batch-to-batch stability and good activity can be obtained.

The application discloses a signal amplification composition for quadruple detection of respiratory pathogens, a detection kit thereof and application, and belongs to the technical field of biology. The signal amplification composition is composed of a fluorescently-labeled AuNS@MoS2-Probe and an amino-modified silica colloidal crystal SiO2 PC-NH2, and realizes double amplification of signals. For low-concentration clinical samples, the application can advance the Ct value, so that samples that need to be rechecked can be directly determined as positive, effectively solves the clinical problem that the signal of a low-concentration sample is weak and is easily submerged by background noise, and reduces the risk of missed detection. The kit is a freeze-dried preparation, has good normal-temperature stability, can simultaneously detect influenza virus A, influenza virus B, a novel coronavirus and mycoplasma pneumoniae, has the advantages of high sensitivity, strong specificity and low missed detection rate, and is suitable for clinical popularization and application.

This invention relates to the field of pathogen infection detection technology, specifically a method for visually tracking human bronchial organoids infected with Mycoplasma pneumoniae. It includes the following steps: (1) Constructing human bronchial organoids: culturing mature human bronchial organoids with cavities, the organoids comprising ciliated cells, basal cells, goblet cells, and Club cells, having a polarized three-dimensional structure with the apex facing outwards; (2) Mycoplasma pneumoniae culture and fluorescent labeling: Mycoplasma pneumoniae is amplified and cultured in MP complete medium, and then fluorescently labeled using CellTrace far-infrared cell staining solution; (3) Co-culture for infection: Mycoplasma pneumoniae fluorescently labeled in step (2) is co-cultured with the human bronchial organoids from step (1) for infection; (4) Live cell dynamic fluorescence tracking: Non-invasive in situ continuous imaging is performed on the human bronchial organoids infected by MP in step (3). This invention can achieve non-invasive dynamic tracking of the entire process of MP infection.

The present application relates to the technical field of microfluidics, and particularly relates to a kit for rapidly detecting 15 respiratory pathogens and a use method thereof.The kit comprises a plurality of reaction cavities, each of which has a primer group corresponding to 15 respiratory pathogens; the primer group comprises inner primers, outer primers and loop primers for PCR amplification of Mycoplasma pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Haemophilus influenzae, Streptococcus pneumoniae, Escherichia coli, Legionella pneumophila, Burkholderia, Staphylococcus aureus, Corynebacterium striatum, Aspergillus, Pneumocystis jirovecii and Candida albicans.The present application can simultaneously detect 15 respiratory pathogens by adding samples once, avoids adding liquid reagents to multiple detection tubes, reduces the intensity of manual operation and the risk of mismatch.

The invention discloses a mycoplasma pneumoniae rapid quantitative detection kit based on nucleic acid extraction-free and application thereof, and belongs to the technical field of nucleic acid detection. The kit comprises a lysis solution, a detection premixed solution, a hydrogel monomer, a positive reference substance, a negative reference substance and a planar reaction chamber. The lysis system provided by the invention does not influence the activity of polymerase while ensuring effective lysis of pathogens, and realizes direct amplification from a clinical swab sample. By combining efficient LAMP amplification with a space-limited hydrogel microenvironment, absolute quantification is realized by fluorescent dot counting. The total detection time of the kit provided by the invention is shortened to be within 30 minutes, and the practicability of the kit in POCT and resource-limited regions is greatly improved.