58 results about "Mycoplasma" patented technology

The invention relates to a temperature calibration method of a nucleic acid amplification analyzer, and relates to the field of instrument calibration, a temperature probe of a standard temperature measuring instrument is placed on the instrument, and point value temperatures of 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C and 100 DEG C are set; starting the data acquisition instrument, respectively operating and heating to temperatures of different target temperature values, calculating an average temperature value of each hole site, and calculating an absolute value of a difference value between the average temperature value and the target temperature value; a mycoplasma pneumoniae quality control product inspection solution with a standard concentration is used, a matched mycoplasma pneumoniae nucleic acid detection kit is added and then put into an instrument to detect a nucleic acid sample, a temperature drift compensation module is arranged, and the deviation of circuit change in long-term use is corrected through periodic self-inspection; the method has the advantages that the difference between the target temperature value and the actual detection temperature value caused by various factors is eliminated, the temperature drift of the instrument in the long-time use process is solved, and the temperature control accuracy of the instrument in the long-time use process is improved.

The invention discloses a primer group and a kit for simultaneously detecting nucleic acids of various respiratory pathogens based on twenty-six PCR (Polymerase Chain Reaction). The kit comprises primer pool freeze-dried powder of influenza A virus (FluA), influenza B virus (FluB), respiratory syncytial virus (RSV), human parainfluenza virus (hPIV), mycoplasma pneumoniae (MP), novel coronavirus (SARS-CoV-2), human respiratory adenovirus (HADV), human respiratory nose / enterovirus (HRV / HEV), bordetella pertussis (BP), human coronavirus (HCoV), human bocavirus (HBoV) and chlamydia pneumoniae (CP), PCR (polymerase chain reaction) freeze-dried powder and a buffer solution. According to the kit, primer system optimization is carried out on various pathogens, brand new primers and probe pools are designed, clinical sensitivity and specificity can be achieved, and meanwhile, the kit is matched with a fully-closed full-automatic detection card box integrating sample treatment and amplification and is suitable for rapid detection of respiratory tract pathogens.

The invention belongs to the technical field of biology, and particularly relates to a primer probe composition and a kit for detecting lower respiratory tract infection pathogens. The primer probe composition is used for detecting mycobacterium tuberculosis, mycobacterium avium, mycobacterium abscessus, mycobacterium kansasii, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii and haemophilus influenzae, and the primer probe composition is used for detecting mycobacterium tuberculosis, mycobacterium avium, mycobacterium abscessus, mycobacterium kansasii, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii and haemophilus influenzae. The composition is one or more of a composition for detecting staphylococcus aureus, a composition for detecting streptococcus pneumoniae, a composition for detecting escherichia coli, a composition for detecting moraxella catarrhalis, a composition for detecting serratia marcescens, a composition for detecting mycoplasma pneumoniae, a composition for detecting candida albicans or a composition for detecting adenovirus; the RAA-RDB technology has the core advantages of rapidness, high sensitivity, low cost and easy operation, and is especially suitable for multiple pathogen screening and basic medical application.

The invention relates to the technical field of sewage purification, and particularly discloses a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The invention relates to a pollution reduction type water quality purification method combining shellfish and grass carp bacteria and algae. The method comprises the following steps: S1, collecting, adjusting and precipitating sewage through a pretreatment unit, and intercepting solid garbage at the same time; s2, introducing the pretreated sewage into a fish-fungus-algae symbiotic unit, putting fungi and algae into the unit, and stocking herbivorous fishes; s3, effluent of the fish-fungus-algae symbiosis unit is guided into a shellfish purification unit, and shellfish is put into the shellfish purification unit; s4, leading effluent of the shellfish purification unit into an aquatic plant purification unit, and planting aquatic plants in the aquatic plant purification unit. The pollution reduction type water quality purification method combining the shellfish and grass carp bacteria and algae can be used for scenes such as village and town domestic sewage, agricultural non-point source pollution and sewage treatment plant tail water, and has the advantages of pollutant reduction and water body self-purification capability reconstruction.

The invention discloses a primer composition for respiratory tract pathogen detection, a related product and application, and belongs to the field of pathogen detection. The primer composition comprises a primer pair aiming at influenza A virus, a primer pair aiming at influenza B virus, a primer pair aiming at bordetella pertussis, a primer pair aiming at adenovirus, a primer pair aiming at respiratory syncytial virus and a primer pair aiming at mycoplasma pneumoniae. The primer composition and related products can be used for simultaneously detecting influenza A viruses (seasonal H1N1, novel influenza A H1N1 viruses (2009), H3N2, H5N1 and H7N9), influenza B viruses (Victoria series and Yamagata series), adenoviruses (group B, group C and group E), respiratory syncytial viruses (subtype A and subtype B), mycoplasma pneumoniae and bordetella pertussis, so that pathogenic pathogens are quickly determined, and the primer composition and related products have important significance on clinical detection.

The invention provides a kit for detecting drug-resistant mutation of mycoplasma pneumoniae based on RPA-CRISPR and application of the kit. The invention provides an RPA primer pair and a crRNA sequence for detecting the drug-resistant mutation A2063G site of the mycoplasma pneumonia 23s rRNA gene, an RPA-CRISPR / Cas13a detection system is established, the detection system formed by the primer pair and the crRNA can accurately detect the drug-resistant mutation A2063G site of the mycoplasma pneumonia 23s rRNA gene within 30-40 minutes under an isothermal condition, the sensitivity and the specificity are very high, and the detection system can be used for detecting the drug-resistant mutation A2063G site of the mycoplasma pneumonia 23s rRNA gene. Amplification detection can be completed only by performing isothermal amplification at the temperature of 39 DEG C without using a variable-temperature instrument like PCR (Polymerase Chain Reaction); in addition, a lateral flow immunochromatography test strip is further combined, so that the on-site instant detection of the mycoplasma pneumoniae drug-resistant mutation can be realized.

The invention relates to the technical field of biology, in particular to a primer probe combination product for respiratory tract infection detection. A plurality of specific primers and self-quenching probes are integrated in a single reaction system; simultaneous, rapid and rapid detection on 15 respiratory pathogens (including influenza A virus, influenza B virus, respiratory syncytial virus, severe acute respiratory syndrome coronavirus 2, mycoplasma pneumoniae, parainfluenza virus, rhinovirus, enterovirus, coronavirus, adenovirus, metapneumovirus, bordetella pertussis, bocavirus, chlamydia pneumoniae and chlamydia psittaci) is realized. The detection is accurate.

The invention provides a nano antibody specifically bound with mycoplasma pneumoniae P1 protein or an antigen binding fragment thereof, which can specifically recognize and bind the mycoplasma pneumoniae P1 protein and has better affinity with the mycoplasma pneumoniae P1 protein. The mycoplasma pneumoniae P1 protein can be used for preparing products for diagnosing, preventing and / or treating mycoplasma pneumoniae P1 protein infection or diseases caused by the mycoplasma pneumoniae P1 protein infection, or detecting the existence or the level of the mycoplasma pneumoniae P1 protein in a sample.

The invention discloses a reagent combination and a kit for detecting four respiratory tract pathogens based on qPCR, and belongs to the technical field of microbiological detection. Wherein the four respiratory tract pathogens are respectively human adenovirus, human bocavirus, bordetella pertussis and mycoplasma pneumoniae; the kit also comprises an optimized PCR (Polymerase Chain Reaction) buffer solution, DNA (Deoxyribose Nucleic Acid) polymerase and UDG enzyme. Through screening processes such as specific primer probe design, reagent formula and reaction system optimization, high sensitivity and high specificity are maintained while rapid detection is performed, and missing detection and misjudgment are effectively avoided.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The utility model relates to an immunological detection kit for mycoplasma pneumoniae in the technical field of immunological detection. The immunological detection kit comprises a main shell, an auxiliary shell is connected to the bottom of the main shell in an embedded mode. A clamping block is fixedly connected to the interior of the main shell; the clamping blocks are symmetrically arranged in the main shell; the supporting plate is placed through a buckling structure of the multiple clamping blocks. A sample pad is arranged at the top of the supporting plate; the sample pad is arranged between the main shell and the supporting plate; the top of the supporting plate is provided with a conjugate dilution pad; the conjugate dilution pad is arranged on one side of the sample pad; the top of the supporting plate is provided with an NC membrane, the NC membrane is arranged on one side of the conjugate dilution pad, the top of the supporting plate is provided with an absorption pad, and the absorption pad is arranged on one side of the NC membrane. And antibodies generated by mycoplasma pneumoniae infection and antibodies caused by other pathogen infection can be accurately distinguished.

This invention relates to a primer and probe composition and kit for detecting Mycoplasma pneumoniae and its drug resistance gene mutations. The primer and probe composition includes: a primer and probe set for detecting the wild type of Mycoplasma pneumoniae; a primer and probe set for detecting the A2063G mutation in Mycoplasma pneumoniae; a probe for detecting the A2064G mutation in Mycoplasma pneumoniae; a primer and probe set for detecting a human gene internal standard; and a competitive blocking agent. The primer and probe composition of this invention can detect Mycoplasma pneumoniae and its A2063G and A2064G drug resistance mutation sites with high sensitivity and specificity, solving the technical problems of false positives and insufficient specificity in existing quantitative real-time PCR for detecting single-base mutations in Mycoplasma pneumoniae.

The invention relates to the technical field of probiotic application, in particular to application of lactobacillus crispatus HC1261 in preparation of a microbial agent for improving female reproductive health and a composition for improving vagina inflammation. The lactobacillus crispatus HC1261 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 16, 2023, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.27355; the microbial inoculum for improving female reproductive health can reduce the load of the ureaplasma urealyticum in the vagina. The lactobacillus crispatus HC1261 can be used for pertinently reducing the load of the ureaplasma urealyticum in the vagina, and can be used for reducing the related health risks of urethritis, cervicitis, pelvic inflammation, infertility and the like caused by the ureaplasma urealyticum.

The present invention discloses a B cell epitope on the surface of a glyceraldehyde-3-phosphate dehydrogenase protein, its nucleic acid molecule, and its application in detecting Mycoplasma hyopneumoniae infection. The B cell epitope of the present invention can be functionally expressed on the surface of an inert carrier S9 bacterium. Based on the LN7-antibody direct-mediated agglutination test detection method established by LN7 functionally expressed on the surface of the S9 bacterium, LN7 specifically recognizes only Mycoplasma hyopneumoniae infection serum antibodies and vaccine immune serum antibodies containing the GAPDH antigen, generating a specific binding reaction, while having no cross-reaction or non-specific binding reaction with other mycoplasma pathogens, porcine respiratory disease pathogen infection serum, and vaccine immune serum. This diagnostic method is expected to become an important technical means for the accurate diagnosis of Mycoplasma hyopneumoniae infection and the monitoring and evaluation of the immune effect of Mycoplasma hyopneumoniae vaccine.

The invention discloses a detection method for simultaneously determining multiple respiratory pathogens. According to the present invention, with the help of the recognized excellent signal amplification tool-CRISPR / Cas12a system and the DNA nanometer machine, the metal element labeling and the ICP-MS detection technology are assisted, such that the simultaneous detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the influenza A virus (H1N1) and the mycoplasma pneumoniae (MP) in the respiratory pathogens is completed so as to easily maintain the respiratory system safety of the whole human. When a compound formed by Cas12a / crRNA is specifically combined with a corresponding target DNA, the trans-shear activity of a CRISPR / Cas12a system can be activated, so that corresponding DNAzyme can be sheared, a DNA nano machine cannot run, lanthanide series metal elements on the DNA nano machine cannot be released, ICP-MS outputs a low signal, and quantitative analysis of multiple DNAs can be realized. The method for detecting the respiratory pathogens can realize simultaneous detection of multiple components, reduces the detection cost and the sample consumption, and has the advantages of high sensitivity and good specificity.

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition for detecting pathogens in a female lower genital tract, a kit and application. The oligonucleotide composition disclosed by the invention can be used for rapidly and simultaneously detecting a plurality of pathogens in gardnerella vaginalis, atriurella vaginalis, prevotella vulgaris, campylobacter mobilis, bacteroides, trichomonas vaginalis and mycoplasma in a sample in a high-specificity manner, and has high sensitivity and high stability; and a rapid, non-invasive, accurate and convenient detection method is provided for female reproductive health detection.

The present invention discloses a nucleic acid detection kit for simultaneously detecting 9 respiratory pathogens based on a common qPCR detection platform, comprising the following non-mixing: reaction solution A, reaction solution B, and reaction solution C. Reaction solution A includes forward and reverse primers and fluorescent probes for influenza A virus, influenza B virus, and respiratory syncytial virus; reaction solution B includes forward and reverse primers and fluorescent probes for human parainfluenza virus, Mycoplasma pneumoniae, and the new coronavirus; reaction solution C includes forward and reverse primers and fluorescent probes for human respiratory adenovirus, human respiratory rhinovirus / enterovirus, and Bordetella pertussis. The fluorescent groups of the fluorescent probes used for different pathogens in the same reaction solution are different. The nucleic acid detection kit for simultaneously detecting 9 respiratory pathogens based on a common qPCR detection platform of the present invention has high specificity and high sensitivity, and is suitable for rapid detection of respiratory pathogens.

The application discloses a detection system of mycoplasma pneumoniae. According to the P1 gene of mycoplasma pneumoniae, RPA primers and crRNA required by a CRISPR system with LbCas12a as a core enzyme are designed and screened, an RPA system and a CRISPR system are established, the influence of each component of the CRISPR-LbCas12a system on the RPA system reaction and the influence of each component of the RPA system on each component of the CRISPR-LbCas12a system are tested, components are optimized according to the test results, and an RPA-LbCas12a integrated system capable of detecting a conserved fragment on the P1 gene of mycoplasma pneumoniae is established. The RPA-LbCas12a integrated system established in the application has high sensitivity, high detection efficiency, reduces nucleic acid pollution, and has a wide application prospect.

The invention belongs to the technical field of research and development of quintuplet vaccines, and particularly relates to a chicken Newcastle disease virus strain LaSota, a chicken Newcastle disease virus strain H120, a chicken Newcastle disease virus strain N49, a chicken Newcastle disease virus strain FADV-4 and a chicken Newcastle disease virus strain HN01. The chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain FADV-4 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain FADV-4 and the chicken Newcastle disease virus strain HN01 are combined. The chicken Newcastle disease virus LaSota strain is proliferated in chicken embryo fibroblasts or chicken kidney cells, and the chicken Newcastle disease virus LaSota strain and the preparation method thereof have the beneficial effects that the chicken Newcastle disease virus LaSota strain and the preparation method thereof can be used for preparing the chicken Newcastle disease virus LaSota strain. The preparation method comprises the following steps: selecting an inactivated newcastle disease virus LaSota strain, an inactivated bronchitis H120 strain, an inactivated bursal disease N49 strain, an inactivated fowl adenovirus FADV-4 strain and an inactivated mycoplasma synovium HN01 strain; it can be guaranteed that a follow-up vaccine has the advantages of being low in toxicity, high in biological safety, high in cross protection capacity, high in immune response speed, long in antibody protection period, high in cellular immune level and high in virus culture content.

The invention provides application of 3-aminophenylboronic acid functionalized magnetic beads in enrichment and / or detection of influenza A virus and / or mycoplasma pneumoniae products, and belongs to the technical field of biological detection. According to the invention, 3-aminophenylboronic acid functionalized magnetic beads are used for broad-spectrum enrichment and / or detection of influenza A virus and / or mycoplasma pneumoniae for sample pretreatment, so that the problems that the existing column chromatography method and magnetic bead method for extracting nucleic acid are tedious in operation, are not beneficial to field application and need assistance of large-scale equipment such as a centrifugal machine and the like; and the problem that pollution is easily caused by repeated uncapping and pipetting depending on professional technicians is solved, and support is provided for on-site rapid detection. The kit disclosed by the invention can specifically detect the influenza A virus and / or the mycoplasma pneumoniae, and the sensitivity of detecting the influenza A virus and / or the mycoplasma pneumoniae is high.

The invention discloses a Mycomonas rosae CCSM007 strain separated from human skin and capable of improving skin health and a metagen of the Mycomonas rosae CCSM007 strain, and belongs to the field of microbial technologies and medicines. The myxomonas rosae CCSM007 provided by the invention has a good barrier function repairing effect and application prospect in the aspect of external use, and the application prospect is specifically shown as follows: the activity of damaged HaCaT cells is improved in vitro, the expression of gene levels and protein levels in the aspects of functional proteins and structural proteins after damage is improved, and meanwhile, the expression of sphingomyelinase 1 (SMPD1) is improved. The metaplast of the myxomonas rosae has a huge application prospect in preparation of cosmetics or medicines for repairing skin barriers.

This invention discloses a multiplex qPCR kit for the simultaneous detection of six respiratory pathogens. The multiplex qPCR kit of this invention can simultaneously detect six common respiratory pathogens: adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, and chlamydia pneumoniae. It has the advantages of high specificity, high sensitivity, good repeatability, and is unaffected by interfering substances. Furthermore, the kit is simple to operate and quick to use for detecting adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, or chlamydia pneumoniae. It can simultaneously detect these six different common respiratory pathogens using a two-tube reaction system, and the detection results are accurate. It is suitable for rapid clinical detection of respiratory pathogens, which helps in timely and accurate treatment of the infecting pathogens.

A kit of detecting Mycoplasma pneumoniae is provided, and the kit comprises a combination of primers and probe consisting of a forward primer, a reverse primer and a probe. The forward primer is selected from the group consisting of SEQ ID NO: 2-3 and complementary sequences thereof, the reverse primer is selected from the group consisting of SEQ ID NO: 6-8 and complementary sequences thereof, and the probe is SEQ ID NO: 9 or a complementary sequence thereof. A method for detecting Mycoplasma pneumoniae using the above kit is also provided.

The invention relates to the technical field of biological detection, and discloses a composition for detecting respiratory tract infection pathogens, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting legionella pneumophila, bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, mycobacterium tuberculosis, aspergillus and cryptococcus neoformans in a sample with high specificity, and has relatively high sensitivity and stability. Compared with traditional detection methods such as pathogen isolated culture and genome sequencing, the efficiency of identifying and jointly detecting multiple respiratory tract pathogens by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, the AUC is 89.8%, and the evaluation model is used for evaluating the quality of polygonatum sibiricum. The method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker primer pair related to swine tolerance mycoplasma pneumonia traits in an HSPA12A gene coding region and application of the SNP marker primer pair. The locus of the SNP marker is a molecular marker of the rs3472181281 nucleotide locus of a pig HSPA12A gene coding region in a reference sequence of an international pig genome version 11.1, and the SNP marker has G / C polymorphism. According to a primer pair for detecting the SNP marker, an upstream primer is SEQ ID NO: 2, and a downstream primer is SEQ ID NO: 3. The SNP marker provided by the invention can be applied to marker-assisted selection of swine mycoplasmal pneumonia resistant traits, and swine populations or strains resistant to swine mycoplasmal pneumonia traits are screened by identifying the genotype of the SNP marker. Establishment of the group or strain can improve the capability of tolerating swine mycoplasmal pneumonia and produce more social and economic benefits.

The present invention provides a recombinant human Mycoplasma pneumoniae adhesion protein vaccine comprising human Mycoplasma pneumoniae adhesion proteins P1, P30, P40 / 90, and P116 fusion proteins C1, C1-G4S, C2, and C2-G4S. Upon immunization of mice with the recombinant human Mycoplasma pneumoniae adhesion protein vaccine, purified and expressed in Escherichia coli, the vaccine induces a strong immune response in vivo, reduces cytokine production, and reduces Mycoplasma pneumoniae colonization in lung tissue. The vaccine can be used for the prevention, detection, or treatment of Mycoplasma pneumoniae infection.

The invention belongs to the technical field of biological detection, and particularly relates to a fluorescence-quenching labeled double-chain universal probe and a mediated amplification method and application thereof. The nucleic acid detection system comprises a fluorescence-quenching labeled double-stranded universal probe, the double-stranded universal probe is composed of two complementary oligonucleotide chains, and the nucleotide sequences of the double-stranded universal probe are shown as SEQ ID NO: 1-4. According to the pathogen nucleic acid detection method based on the universal probe and the target specific anchoring primer, a real-time quantitative PCR or isothermal amplification technology and a fluorescence resonance energy transfer mechanism are integrated, and synchronous and accurate detection of mycoplasma pneumoniae, streptococcus pneumoniae, haemophilus influenzae, influenza A virus, influenza B virus, novel coronavirus and the like is achieved. The method does not need pathogen culture, solves the problems of insufficient specificity, high cost, tedious operation and the like of a traditional detection technology, and can be applied to rapid diagnosis of clinical pathogen infection, reasonable use guidance of antibiotics and epidemiological monitoring.

The present application relates to a SNP marker primer pair related to a pig mycoplasma pneumonia tolerance trait on pig chromosome 15 and application thereof. The SNP marker site is a molecular marker of nucleotide site rs1110963643 on pig chromosome 15 in the international pig genome 11.1 version reference sequence, and has T / C polymorphism. A primer pair for detecting the SNP marker, the upstream primer is SEQ ID NO: 2, and the downstream primer is SEQ ID NO: 3. The SNP marker provided by the present application can be applied to marker-assisted selection of pig mycoplasma pneumonia tolerance traits, and the pig population or strain with tolerance to pig mycoplasma pneumonia is screened by identifying the genotype of the SNP marker. The establishment of the population or strain can improve the ability to tolerate pig mycoplasma pneumonia, and produce more social and economic benefits.

The present invention pertains to a method for detecting microorganisms such as mycoplasma or mouse minute virus (MMV) in a sample, the method comprising: (i) performing PCR on a sample suspected of comprising mycoplasma DNA with a PCR reaction mixture comprising (1) a first primer (Pl) annealing to the reverse complement of a nucleic acid sequence of SEQ ID NO: 1 and a second primer (P2) annealing to a nucleic acid sequence of SEQ ID NO: 2, or (2) a third primer (P3) annealing to the reverse complement of a nucleic acid sequence of SEQ ID NO: 109 and a fourth primer (P4) annealing to a nucleic acid sequence of SEQ ID NO: 110; and (ii) detecting a PCR product, wherein the detection of a PCR product indicates that the sample comprises microroganisms, and the absence of a PCR product indicates that the sample does not comprise microroganisms. The present invention further pertains to a pair of primers for detecting mycoplasma or virus in a sample, and to a kit for detecting mycoplasma or virus in a sample.