The invention belongs to the technical field of
plant propagation, and specifically relates to a cultivation method for a novel variety by inducing
mutation of tender stem tissues of
epimedium koreanum. According to the cultivation method for the novel variety by inducing
mutation of the tender stem tissues of the
epimedium koreanum provided by the invention, with tender stems of the
epimedium koreanum materials, by combination of
ethyl methanesulfonate (EMS) with a
plant tissue culture technology and adoption of methods of embryonic
callus induction, redifferentiation, embryoid rejuvenation and the like, an artificially cultivated novel variety of the epimedium koreanum, which is strong in independence of each
single plant, high in genetic information purity of each
single plant and clearin
single plant source, is obtained; and the cultivation method has important protection and
ecological significance for amplification of an epimedium koreanum
population; meanwhile, the cultivationmethod can be further applied to construction of an epimedium koreanum
mutant library; and the novel variety cultivated by using the cultivation method provided by the invention can be directly used as a strain for cultivation and variety certification.