59 results about "Intestinal membrane" patented technology

Transgenic chloroplast technology could provide a viable solution to the production of Insulin-like Growth Factor I (IGF-I), Human Serum Albumin (HSA), or interferons (IFN) because of hyper-expression capabilities, ability to fold and process eukaryotic proteins with disulfide bridges (thereby eliminating the need for expensive post-purification processing). Tobacco is an ideal choice because of its large biomass, ease of scale-up (million seeds per plant), genetic manipulation and impending need to explore alternate uses for this hazardous crop. Therefore, all three human proteins will be expressed as follows: a) Develop recombinant DNA vectors for enhanced expression via tobacco chloroplast genomes b) generate transgenic plants c) characterize transgenic expression of proteins or fusion proteins using molecular and biochemical methods d) large scale purification of therapeutic proteins from transgenic tobacco and comparison of current purification/processing methods in E. coli or yeast e) Characterization and comparison of therapeutic proteins (yield, purity, functionality) produced in yeast or E. coli with transgenic tobacco f) animal testing and pre-clinical trials for effectiveness of the therapeutic proteins. Mass production of affordable vaccines can be achieved by genetically engineering plants to produce recombinant proteins that are candidate vaccine antigens. The B subunits of Enteroxigenic E. coli (LTB) and cholera toxin of Vibrio cholerae (CTB) are examples of such antigens. When the native LTB gene was expressed via the tobacco nuclear genome, LTB accumulated at levels less than 0.01% of the total soluble leaf protein. Production of effective levels of LTB in plants, required extensive codon modification. Amplification of an unmodified CTB coding sequence in chloroplasts, up to 10,000 copies per cell, resulted in the accumulation of up to 4.1% of total soluble tobacco leaf protein as oligomers (about 410 fold higher expression levels than that of the unmodified LTB gene). PCR and Southern blot analyses confirmed stable integration of the CTB gene into the chloroplast genome. Western blot analysis showed that chloroplast synthesized CTB assembled into oligomers and was antigenically identical to purified native CTB. Also, GM1,-ganglioside binding assays confirmed that chloroplast synthesized CTB binds to the intestinal membrane receptor of cholera toxin, indicating correct folding and disulfide bond formation within the chloroplast. In contrast to stunted nuclear transgenic plants, chloroplast transgenic plants were morphologically indistinguishable from untransformed plants, when CTB was constitutively expressed. The introduced gene was stably inherited in the subsequent generation as confirmed by PCR and Southern blot analyses. Incrased production of an efficient transmucosal carrier molecule and delivery system, like CTB, in transgenic chloroplasts makes plant based oral vaccines and fusion proteins with CTB needing oral administration a much more practical approach.

The invention discloses a method for preparing functionality feed protein intestinal membrane albumen powder, which solves the problems that simultaneous hydrolyzing of multienzyme can affect the hydrolyzing degree and the acquiring rate of the protein peptide, more particularly, the lower acquiring rate of small peptide in the existing intestinal membrane albumen powder preparing technique. The technical scheme includes (1) treating before materials; (2) adding protease to hydrolyze; (3) adding a carrier; (4) preparing through sponging drying; wherein, in the step of hydrolyze the protease, hydrolyzing is carried through on any two of neutral protease, pawpaw protease and alkali protease by adopting a multienzyme grading hydrolyzing method. The functionality feed protein intestinal membrane albumen powder prepared by the method has the obvious advantages in the aspects of the acquiring rate of the protein peptide and the acquiring rate of the small peptide. The invention mixed to feed with other feeds can remarkably improve the performance trait of immature animals.

The invention discloses a suckling pig creep feed mixed feed which consists of the following raw materials in percentage by mass: national standard level-1 corn, national standard level-1 wheat, extruded full-fat soybean, extruded dehulled soybean meal, concentrated soybean protein, defatted fish meal, high-protein whey powder, edible glucose, high-quality soybean oil, coconut oil, calcium lactate, feed grade calcium hydrophosphate and gunk. The mixed feed is prepared by employing the production process of superfine grinding, mixing, pelletizing, cooling, grading and packaging. According to the suckling pig creep feed mixed feed, the conventional problems of low suckling pig creep feed protein, little suckling pig feed intake, high feed conversion ratio and slow weight increase within 10 days after breast-feed and ablactation are solved, the plasma proteins, intestinal membrane proteins and other protein raw materials homologous with pig organisms which bring danger of porcine virus diseases and the risk of possibly influencing and changing porcine biogenic genes are avoided, the suckling pig feed intake and daily gain are improved, and the feed conversion ratio is reduced.

The invention relates to a transportation technique of non-Newtonian fluid-Pingham body, in particular to a pipeline conveying method of viscosity plastic material, which comprises a processing method of inner wall of pipeline, a method of forming capsules of the material, a formulating method of additives and the like. A surface film drag reduction method of bionic fish body surface film and intestinal membrane is employed to design a pipeline for reducing wall friction, a surface film is arranged between the material and the pipeline to enable the inner friction of the material to be larger than that of the pipe wall, and to enable the viscosity plastic material with high concentration, high viscosity and low strength to be transported under the premise of low energy consumption, and the difficult problem soft and sticky material for long-distance pipeline transportation is resolved. The transportation technique of non-Newtonian fluid-Pingham body has the advantages of large transport capacity, long transport distance, low consumption and wide market, and is adaptable to a plurality of aspects, such as petroleum, chemical industry, mining, environmental protection, construction and the like.

The invention relates to a process for preparing intestinal membrane protein powder by utilizing residual liquid sourced from heparin sodium production, which comprises the following steps of: (1) raw material pretreatment: filtering heparin sodium residual liquid by nylon cloth of 180 meshes for removing impurities; (2) raw material desalination and dehydration: desalinating and dehydrating raw materials by the heparin sodium residual liquid obtained, after impurity removal, through nanofiltration membrane equipment; (3) enzymolysis: adding composite animal protein hydrolase which is 0.2-0.8 percent by substrate weight into the heparin sodium residual liquid with the substrate concentration of 3-6 percent, adjusting the pH value to 7.0 to 8.5 and carrying out enzymolysis at the constant temperature of 50-60 DEG C for 4-8 hours; (4) carrier addition: adding wheat bran which accounts for 20-50 percent by substrate weight into enzymolysis liquid, completely dissolving and uniformly stirring; and (5) spraying and drying. The method has the advantages of simple process, low cost, enzymolysis thoroughness, high product yield and high product quality, can be used for reducing the environmental pollution, changing the waste materials into valuable things and reducing the production energy consumption and the production cost.

The invention provides a production and processing process of intestinal membrane albumen powder. By taking intestinal mucosa of a pig or intestinal residues with heparin extracted as a raw material, the process comprises the following steps: 1) after mixing water and the raw material in a weight ratio of (15-30) to 100, stirring for 30-60 minutes at 35-40 DEG C; 2) adding alkaline protease into the mixture of water and raw material in the step 1) in a weight ratio of (0.05-1):100, heating to 40-60 DEG C, adjusting the pH to 9.0-11.0, and preserving heat and hydrolyzing for 1-4 hours at 40-60 DEG C to obtain a hydrolysate; 3) spray drying or roller drying the hydrolysate to prepare the intestinal membrane albumen powder. The process of preparing the intestinal membrane albumen powder by using a small amount of water and alkaline protease under the alkaline condition has the characteristics of simple operation, short hydrolytic time, energy conservation and environmental protection, small use level of water and alkaline protease, low preparation cost and the like, and can satisfy the requirements on industrialized production on a large scale.

The invention provides a preparation method of an ocular surface biofilm, which uses animal pericardium, intestinal membrane and adipose omentum as raw materials through degreasing, decellularization, multi-directional antigen removal, epoxy fixation, curved surface molding, surface Modification, cobalt-60γ-ray irradiation sterilization and other process steps. The ocular surface biofilm obtained by the method of the present invention has a curved surface similar to that of the eyeball, can be close to the eyeball, has no antigenicity, good histocompatibility, can participate in the healing process of ocular surface wounds, and is beneficial to wound healing; Good air permeability and bacterial filtration can play a good role in the protection and treatment of the ocular surface; it has good strength, can be pasted and sewed, and is easy to use. In addition, through the surface modification of drug sustained release, it can also be made into a therapeutic ocular surface biofilm that can release anti-infective drugs slowly.

The invention discloses a suckling pig teaching slot material and a preparation method thereof. The suckling pig teaching slot material comprise the following raw materials by weight: 20-25 units of flour corn, 15-20 units of high-quality soybeans, 10-15 units of broken rice, 2-5 units of 46% bean pulp, 8-12 parts of high protein flour, 10-15 units of 12% whey powder, 5-8 units of first class puffed fishmeal, 2-5 units of milk powder, 4-8 units of compound monosaccharide and disaccharide, 2-5 units of blood plasma intestinal membrane, 2-5 units of emulsified medium and long chain fatty acid oil and 2-5 units of other condiments. The preparation method of the suckling pig teaching slot material comprises five steps of smashing, puffing, mixing and spraying oil, hardening and tempering and granulating, weighing and packaging. The suckling pig teaching slot material is scientific in preparation method. The prepared suckling pig teaching slot material has the advantages of comprehensive and balanced nutrition, easy digestion, easy absorption and the like, has the aromatic, soft and crisp characteristic, can significantly improve palatability of the suckling pig teaching slot material, thereby obviously increasing food consumption of the suckling pig, enabling the suckling pig to be health, to increase weight rapidly without diseases and to reduce death rate, and increasing culture benefits obviously.

The present invention discloses a plasma protein powder free weaned piglet milk replacer. The milk replacer consists of the following proportioned raw materials in parts by weight: ordinary corn 9.1 parts, extruded corn 5 parts, rice 12.5 parts, shortening 1 part, extruded soybean 12.5 parts, soybean meal 8 parts, soybean oil 1 part, white granulated sugar 3 parts, fish meal 4.5 parts, calcium hydrogen phosphate 0.4 part, stone powder 0.5 part, lysine 0.45 part, methionine 0.05 part, threonine 0.15 part, tryptophan 0.05 part, choline 0.15 part, monosodium glutamate 0.2 part, cupric sulfate 0.06 part, trace mineral elements 0.2 part, yolk antibody 0.105 part, acidifier 0.5 part, mildew-proof agent 0.1 part, antioxidant 0.04 part, maltodextrin 10 parts, trace vitamins 0.4 part, flour 12.65 parts, enzymic preparation 0.13 part, glucose 5 parts, intestinal membrane protein powder 3 parts, composite yeast 1.5 parts, whey powder 7.5 parts, flavouring agent 0.1 part, sweetener 0.05 part and micro ecological preparation 0.03 part. The milk replacer avoids the risk of homologous biological safety.

The invention relates to a method for simultaneously extracting heparin sodium and intestinal membrane protein peptide based on an enzymolysis method. The method comprises the following steps of: preparing an intestinal mucosa mixed solution; preparing enzymatic hydrolysate by adopting an enzymolysis method; filtering to obtain clarified filtrate and an enzymolysis concentrated solution; performing heparin adsorption on the filtrate through an ion exchange resin column, and obtaining effluent at the same time; taking down the adsorbed and saturated ion exchange resin column, firstly cleaning the resin in the ion exchange resin column by using cleaning saline water, and then performing heparin elution on the resin by using desorption saline water to obtain heparin sodium eluent; filtering,desalting and concentrating the heparin sodium eluent to obtain a heparin sodium concentrated solution; drying the heparin sodium concentrated solution to obtain crude heparin sodium; cooling the effluent, and then carrying out desalination and concentration to obtain a first concentrated solution; carrying out vacuum concentration on the first concentrated solution to obtain a protein peptide concentrated solution; and drying the protein peptide concentrated solution to obtain the protein peptide. The method is high in heparin sodium extraction rate, short in production period, capable of extracting protein peptide at the same time, low in cost and low in pollution.

The invention discloses feed a milk replacer using cured grain raw materials to improve production performance of piglets. The milk replacer comprises the following components: 0-40% of corn; 10-50% of cured corn; 3-10% of soybean meal; 2-6% of fermented soybean meal; 2-5% of intestinal membrane protein; 2-10% of whey powder; 2-5% of super fish meal; 1.5-5% of spray dried plasma protein powder; 3-8% of glucose; 0.3-0.4% of an acidifier; and 4% of a 4% pre mixed feed; and also can be added with 5-10% of cured soybean, 5-15% of cured rice or broken rice, and 1-2% of soybean oil; wherein the cured corn, cured soybean and cured rice or broken rice are obtained by curing processing of corn, soybean, rice or broken rice by a curing processing method. The milk replacer prepared by use of the cured grain raw materials further improves the feed digestibility, and increases feed intake of the piglets, and weaning piglets have no stress, and may not lose weight and stop growth; so that the development and growth of the piglets are accelerated, and the production performance of the piglets is improved.

The invention relates to the technical field of food processing, in particular to a continuous production method of potato fermented beverage and potato residue powder and products prepared by the continuous production method. Potato raw materials are subjected to enzymolysis and fermented with probiotics such as lactobacillus rhamnosus, pediococcus pentosaceus and leuconostoc mesenteroides, supernate obtained through fermentation and precipitate are separated, the supernate is blended into the potato fermented beverage, and the precipitate is blended into the potato fermented residue powder. The fermented beverage provided by the invention is rich in sweet potato flavor, rich in short-chain fatty acid, soluble protein, small-molecule active peptide and the like, and has potential effects of resisting oxidation, diminishing inflammation, enhancing the immunity of a body, regulating blood pressure and the like; and the potato fermented residue powder is rich in soluble dietary fibers, the problems that traditional potato residues are rough in palatability, poor in flavor and the like are solved, and the dietary fiber structure can be improved, the diversity of probiotic flora of the intestinal tract and the normal function of the intestinal tract can be maintained, and the health of the intestinal tract can be promoted.

The invention discloses a combined technology for extracting heparin sodium and intestinal membrane protein from small intestines of pigs and obtaining finished product casing. According to the technology, in an extraction process, extraction raw materials resin and ethanol are recycled; by scraping intestinal mucosa on small intestines of pigs, heparin sodium is adsorbed by a resin after enzymolysis of porcine pancreas, and a purified heparin sodium product is obtained after salt solutions with different concentrations and ethanol precipitation; continuous enzymolysis is performed on the supernatant, and then membrane concentration and drying are performed to obtain high-purity intestinal membrane protein; and leakage detection and pickling are performed on casings to obtain finished product casings. Raw materials of small intestines of pigs in the Chinese slaughter industry are easy to obtain, the operation mechanization degree is high, the raw materials can be recycled, the sausagecasing utilization rate is high, the method is suitable for large-scale industrial production, and the obtained heparin sodium and intestinal membrane protein are high in purity, good in economic benefit and wide in prospect.

The invention discloses a preparation method of safe and healthy stewed mutton with good mouthfeel and taste, wherein the preparation method comprises the following steps: washing fresh mutton cleanly, freezing, thawing and then cutting into blocks, sending the mutton blocks into clear water, soaking, fishing out, and draining, to obtain a first prefabricated material; mixing lemon, cassia bark, nutmeg, cumin, radix angelicae, raw ginger and bay leaf, then wrapping the mixture with gauze, adding clear water, mixing and boiling, then cooking for 10-20 min with slow fire, adding brown sugar, soy sauce, table salt, yellow wine, chicken essence, dried chilli pepper, vanillin, a monascus red pigment and sodium nitrite, cooking, cooling, adding the first prefabricated material, carrying out heat preservation, fishing out, and draining, to obtain a second prefabricated material; sending the second prefabricated material into a baking room, baking, adding brown sugar, soy sauce, table salt, yellow wine, chicken essence, raw ginger, coriander, ethyl maltol, a monascus red pigment and water, cooking, fishing out, and draining, to obtain a third prefabricated material; and freezing to -1 to -5 DEG C, adding gelatin and water, mixing evenly, carrying out intestinal membrane vacuum filling, packaging, sterilizing at high temperature, and thus obtaining the safe and healthy stewed mutton with good mouthfeel and taste.

The invention discloses a novel process for machining pig and sheep intestinal membrane protein. The process comprises the following steps: purchasing fresh pig and sheep pancreas, duodenum and gall bladder from a slaughter house every day; washing; preserving at a low temperature for later use; smashing the pancreas, the duodenum and the gall bladder together in a stamp mill; adjusting the moisture of the intestinal membrane protein to be subjected to enzymolysis to be 70-86%; subsequently heating to 86-95 DEG C and keeping the temperature for 20min; further introducing circulation cooling water to cool to 33-40 DEG C and keeping the temperature; adjusting pH value to 7-9 by using baking soda; putting the smashed pancreas mixture into an enzymolysis tank in a percentage of 1%; constantly stirring at a slow speed (2 cycles/minute) at 37 DEG C for 2 hours; and puffing the intestinal membrane protein at 110-130 DEG C by using a wet method. The intestinal membrane protein which is subjected to enzymolysis and puffing is mixed in a ratio of 1:3, and is dried up. According to the process, rich intestinal membrane protein resources are utilized and machined in an enzymolysis and puffing mode, so that the molecular structure of a product is loose, the ratio of low molecular peptide protein and free amino acid obtained from enzymolysis is greatly increased, and the product is sterilized and disinfected, so that the novel, efficient and safe process for machining the intestinal membrane protein is realized ultimately.

The invention discloses a preparation method and application of an intestinal membrane peptide. According to the method, the problems of great production odor, high salinity, high water content and single function of intestinal membrane stoste, namely intestinal membrane liquid I are solved. By adopting the steps of filtering the intestinal membrane stoste, adjusting a pH value, deodorizing, desalting and dehydrating a biological membrane, performing biological enzymolysis, drying, packaging products and the like, the content of small peptide in a produced intestinal membrane peptide product is greater than 50 percent (the molecular weight is smaller than or equal to 1000Da), and the product is easily digested and absorbed and can be used for promoting digestion and absorption of amino acid and mineral substances. The intestinal membrane peptide disclosed by the invention can be used for an animal feed additive and a functional protein raw material.

The invention discloses high-performance environmentally-friendly crab feed and a preparation method thereof. The high-performance environmentally-friendly crab feed is characterized by being prepared from the following raw materials in parts by weight: 10-13 parts of intestinal membrane protein, 19-24 parts of snails, 20-25 parts of NP-2002 plasma protein powder, 1-2 parts of sodium benzoate, 1.2-3.5 parts of kanamycin, 21-27 parts of fly maggots, 6-9 parts of peanut oil, 18-26 parts of soybean cake, 10-11 parts of sweet pepper, 17-19 parts of celery, 16-18 parts of watercress, 0.3-0.6 part of radix gentianae, 0.4-0.7 part of cyrtomium rhizome, 12-15 parts of spina date seed, 5-7 parts of saponin, 1-3 pars of threonine, 1-2 parts of lysine, 3-7 parts of potassium chloride, 2-4 parts of sodium sulphate, 3-6 parts of a phagostimulant and a right amount of water; according to the crab feed provided by the invention, the snails, fly maggots, NP-2002 plasma protein powder and other raw materials are used to replace fish meal, meat and bone meal and other protein raw material, in addition, the soybean cake, the spina date seed and the like are added, so that the quality of the feed is improved, the production cost is reduced, and the safety rate of the breeding food is increased.

The present invention discloses a paste milk replacer product and a preparation method thereof. The paste milk replacer product comprises 15-20 parts of maltodextrin, 25-32 parts of puffed broken rice powder, 10-15 parts of spray-dried enzymolysis soy protein powder, 6-8 parts of plasma protein powder, 4-6.5 parts of soluble spray-dried enzymolysis intestinal membrane protein powder, 4-6.5 parts of spray-dried acidified degraded corn protein powder, 5-6.5 parts of spray-dried soluble fish protein powder, 20-25 parts of low-protein whey powder, 6-8 parts of glucose powder, 6-8 parts of sucrose powder, 3-5 parts of emulsified oil, 0.5-1 part of efficient cream, 0.5-1 part of citric acid, 0.5-1 part of 85% of lactic acid, 0.5-1 part of fructo-oligosaccharide and 0.5-1 part of isomaltooligosaccharide. The preparation method is as follows: spraying the emulsified oil into the puffed broken rice powder to prepare an intermediate material, uniformly mixing the intermediate material with other raw materials, adding 0.55 time of water and stirring the mixture into a paste state, and filling the paste to get the finished product. According to the present invention, the paste milk replacer product has high energy density and high feed digestibility; weak piglets can be rescued by using a tubular feeding method; and the overall survival rate, piglet health rate and uniformity in large-scale pig raising are significantly improved.

The invention discloses a feed additive for improving stress of piglets, and belongs to the technical field of feed. The feed additive comprises the following substances in parts by weight: 30-40 parts of corn meal, 20-30 parts of intestinal membrane protein powder, 20-30 parts of resistant dextrin, 1-10 parts of galactooligosaccharide, 0.5-2 parts of Bifidobacterium bifidum, 0.5-2 parts of bifidobacterium longum, 0.5-2 parts of Bifidobacterium lactis, 0.5-2 parts of Lactobacillus rhamnosus, 0.5-3 parts of vitamin complex, 0.1-3 parts of mineral substances and 0.5-3 parts of Chinese medicine additives. According to the feed additive, various composite probiotics are added, and through the specific ratio of different types of probiotics, beneficial bacteria in organism intestinal tracts ofthe piglets can form a powerful dominant population, and thus, pathogenic bacteria are inhibited and eliminated; a large amount of nutrient substances including amino acids, proteins, vitamins, various biochemical enzymes, growth-promoting factors and the like are secreted and synthesized so as to adjust and improve functions of all parts of the organisms of piglets, and the stress resistance of the piglets is improved. The Chinese medicine additive can exert the functions of nerve soothing and sedation, and can reduce the stress response while improving the immunity of the piglets.

The invention discloses a preparation method of intestinal membrane protein powder, comprising the following steps: (1) cutting fresh chicken intestines into chicken intestine crumbs, grinding the chicken intestine crumbs to prepare chicken intestine raw material slurry after cleaning; (2) deoiling the chicken intestine raw material slurry prepared in the Step (1); (3) deodorizing the chicken intestine raw material slurry; (4) homogenizing the material; 5) carrying out enzymatic hydrolysis treatment on that material; and 6) spray-drying. By the preparation method of the invention, the chickenintestine by-product in slaughterhouses can be efficiently utilized, and the chicken intestine is made into an intestinal membrane protein product. By feeding pigs with the intestinal membrane proteinproduct prepared from chicken intestines, the hidden danger of homologous biological safety is greatly reduced. The intestinal membrane protein product has no impurities, removes grease, has long storage time, high content of small peptides, greatly eliminates the original odor, improves the sensory sense of the product and the feeding habit of pigs, has low production cost, and is beneficial toenvironmental protection.

The invention discloses a method for treating sausage casing wastewater. The method comprises the following steps of: 1) sterilizing; 2) carrying out solid-liquid separation to obtain filtrate and protein-rich solid contents; 3) desalting, wherein the filtrate is passed through a nanofiltration device to obtain high salinity wastewater, amino acid solution and peptide solution; multiple-effect continuous crystallization is carried out on the high salinity wastewate to obtain crystallized salt and sausage casing recycle water; 4) crushing and pulping, wherein the protein-rich solid contents arecrushed and ground to obtain pulp; 5) carrying out enzymolysis, wherein amino acid, peptide solution and pulp are mixed and enzymolysis is carried out on the mixture; 6) concentrating, wherein the mixed solution is concentrated to obtain a concentrated material; and 7) carrying out spray drying, wherein the concentrated material is mixed with auxiliary materials, and spray drying is carried out to obtain an intestinal membrane protein powder. The invention provides the method for treating sausage casing wastewater, which combines a nanofiltration technology, a biological enzyme technology, ahigh salinity wastewater treatment technology and a spray drying technology; the method can meet requirements of sausage casing wastewater treatment, can bring economic benefits to enterprises, and ispracticable.