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Method for preparing intestinal mucosa nutrient peptide microspheres by using wheat hydrolyzed proteins

A technology of wheat hydrolyzed protein and peptide microspheres, applied in protein food processing, protein food ingredients, applications, etc., can solve problems such as low solubility, limited functional characteristics, etc., to improve the function of digestive organs, optimize intestinal nutrition, The effect of promoting growth and development

Inactive Publication Date: 2018-09-18
WUHAN CHORTLE BIO CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a protein with unique properties, the tight three-dimensional network structure and amino acid binding mode of wheat hydrolyzed protein lead to its extremely low solubility, which limits the exertion of functional properties

Method used

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  • Method for preparing intestinal mucosa nutrient peptide microspheres by using wheat hydrolyzed proteins
  • Method for preparing intestinal mucosa nutrient peptide microspheres by using wheat hydrolyzed proteins
  • Method for preparing intestinal mucosa nutrient peptide microspheres by using wheat hydrolyzed proteins

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Preparation of Enteric Membrane Nutritional Peptide Microspheres

[0028] 1. A method for preparing intestinal membrane nutritional peptide microspheres by utilizing wheat hydrolyzed protein, comprising the following steps:

[0029] (1) Configure wheat hydrolyzed protein feed liquid

[0030] Add water to 10g of wheat hydrolyzed protein raw material until the protein concentration is 5%-10%, adjust the temperature of the suspension to 60°C, and use magnetic stirring method to continuously stir for 30-45min to form a uniform suspension for later use;

[0031] (2) Step-by-step enzymatic hydrolysis

[0032] Adjust the pH value of the suspension in step (1) to 7.0±0.5, maintain the temperature at 50±2°C, add Protamex compound protease with a mass of 1.5-2.5% of the suspension to carry out enzymatic hydrolysis for 1 hour, and place the reaction solution in a water bath at 95°C Inactivate the enzyme for 15 minutes, adjust the pH value to 8.5, and the temperature i...

Embodiment 2

[0049] Example 2: Research on the effect of intestinal membrane nutritional peptide microspheres on the intestinal membrane of young

[0050] Experimental grouping: Group A: wheat hydrolyzed protein powder; Group B: small molecule target peptide (the medium and small molecule target peptide of the present invention is freeze-dried or spray-dried to remove moisture to make small molecule target peptide powder); C group: intestinal membrane nutrition Peptide microspheres; Group D: 0.9% saline

[0051] (1) Study on the effect of intestinal membrane nutritional peptide microspheres on the intestinal membrane of piglets

[0052] Twelve 30-day-old piglets with similar body weight were taken and divided into 4 groups on average. In the experiment, group A: fed wheat hydrolyzed protein powder (3g / kg / d); group B: fed wheat glutamine peptide powder (3g / kg / d); group C: fed intestinal membrane nutrient peptide microspheres ( 3g / kg / d); Group D: 3ml of 0.9% normal saline was fed. From the...

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Abstract

The present invention provides a method for preparing intestinal mucosa nutrient peptide microspheres by using wheat hydrolyzed proteins, and belongs to the technical field of wheat hydrolyzed proteindeep processing. The method comprises the following steps: (1) a wheat hydrolyzed protein material solution is prepared; (2) Protamex compound protease and Alcalase alkaline protease are selected toconduct stepwise enzymatic hydrolysis; and centrifugal ultrafiltration is conducted to obtain small molecular target peptides; (3) the small molecular target peptides are subjected to a drying treatment to obtain wheat glutamine peptides; and (4) the wheat glutamine peptides are coated with sodium alginate-chitosan to prepare the intestinal mucosa nutrient peptide microspheres. The intestinal mucosa nutrient peptide microspheres reduce an absorption rate of glutamine entering body by controlling a release rate of the glutamine from the microspheres, and thus play better intestinal nutrient effects. The intestinal mucosa nutrient peptide microspheres can improve intestinal mucosa structures of animals, improve functions of digestive organs, increase length of intestinal villi of ileum, duodenum, etc., improve intestinal environment, promote the growth and development of the intestinal villi, and increase absorption of nutrients by intestinal tracts.

Description

technical field [0001] The invention belongs to the technical field of deep processing of wheat hydrolyzed protein, and relates to a method for preparing intestinal membrane nutrient peptide microspheres by utilizing wheat hydrolyzed protein. Background technique [0002] Glutamine is a "conditionally essential amino acid" in the body under stress. It is an essential nutrient for the metabolism of intestinal mucosal cells and is very important for maintaining the structural integrity of intestinal mucosal epithelium. Especially under severe stress conditions such as fatigue, infection, trauma, etc., glutamine in intestinal mucosal epithelial cells is quickly depleted. When the intestinal tract lacks glutamine or is stimulated by food and digestive juices, the intestinal mucosa will shrink, the villi will become thinner, shorter or even fall off, and the intestinal immune function will be severely impaired. Clinical practice has proved that providing glutamine parenterally c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34
CPCA23J3/346
Inventor 胡金平吴怀祥
Owner WUHAN CHORTLE BIO CHEM TECH
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