482 results about "Target peptide" patented technology

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention discloses an enzyme response type KRAS targeted protein degradation chimera as well as a preparation method and application thereof, and belongs to the technical field of tumor targeted therapy. The enzyme response type KRAS targeted protein degradation chimera disclosed by the invention is obtained by covalently linking three parts, namely a KRAS targeted protein degradation chimera, an enzyme response type amino acid sequence and a cell penetrating peptide amino acid sequence, wherein the KRAS targeted protein degradation chimera is obtained by linking a KRAS targeted peptide and a VHL E3 ligase ligand through succinic acid. The enzyme response type KRAS targeted protein degradation chimera releases the KRAS targeted protein degradation chimera under the action of ATG4 enzyme shearing, and shows a relatively strong lung cancer resisting effect in vitro and in vivo. The invention has significant advantages in the aspect of antitumor drugs, and opens up a new field for the development of targeted protein degradation chimera drugs. Enzyme response type KRAS targeted protein degradation chimera molecular structural formula as follows: # imgabs0 #

The invention provides a preparation method of tiall peptide, and belongs to the technical field of polypeptide synthesis, according to the preparation method, a small amount of lysis solution is adopted for lysis, a small amount of poor solution composed of methyl tert-butyl ether and n-heptane with the volume ratio of 1-2: 1 is used for crude peptide precipitation, precipitated particles are uniform, large and not prone to caking, the crude peptide impurity level is lower, and the purity of the crude peptide is higher. The method has the advantages that the method is simple in process and easy in subsequent purification step, the amount of generated waste liquid is small, the activating agent 7-(1H-pyrrolo [2, 3-b] pyridine-1-yl)-1H-benzo [d] [1, 2, 3] triazole-1-alcohol is used for activating amino acid, the coupling reaction degree is high, and the target peptide yield is high. The preparation method of the tilpotide has the advantages of being simple, economical, capable of achieving mass production, low in impurity content, high in purification yield, high in yield and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to a wheat peptide with antioxidant stress activity and a preparation method and application thereof. The invention provides a wheat peptide with antioxidant stress activity. The amino acid sequence of the wheat peptide is as shown in SEQ ID NO: 1. The invention discloses a wheat peptide FGWPGPK for protecting oxidative stress nerve cells as well as a screening method and application of the wheat peptide FGWPGPK. A target peptide fragment is identified from wheat germs through enzymolysis and multi-dimensional screening strategies, and it is proved that the target peptide fragment can significantly improve the survival rate of PC-12 cells damaged by H2O2 (72.88%) and reduce the ROS level (125.54%). The peptide fragment has strong antioxidant activity and nerve targeting property, and is suitable for preventing and treating neurodegenerative diseases such as Alzheimer's disease.

The present disclosure relates to targeting moieties such as peptides that can bind to B7-H3. The disclosure also provides targeting constructs, which may include a targeting moiety attached, via an optional linker, to a chelating agent for association of a cargo. Methods of making the constructs and formulations thereof are also provided. Methods of using the constructs and / or formulations thereof to treat subjects, for example, to treat or prevent cancer, are also described.

The invention discloses a multifunctional nano delivery system based on targeted ferritin as well as a preparation method and application of the multifunctional nano delivery system. According to the system, traditional Chinese medicine active ingredients, namely neogambogic acid GNA, a photosensitizer Ce6 and ferritin targeting peptide HKN15 are integrated through a self-assembly technology, a synergistic treatment system integrating photodynamic therapy PDT and ferroptosis induction is constructed, and the treatment effect on non-small cell lung cancer NSCLC can be remarkably enhanced. According to the nano delivery system, precise targeting of a tumor site is achieved through the ferritin targeting peptide HKN15, and a double anti-tumor mechanism is formed by utilizing the photodynamic effect of the photosensitizer Ce6 and the ferroptosis induction effect of GNA. The preparation process is based on a molecular self-assembly principle, and has the advantages of high tumor accumulation efficiency, low system toxicity, remarkable tumor inhibition effect and the like, and an innovative solution is provided for precise treatment of the non-small cell lung cancer.

The invention discloses a TREM-1 inhibitory peptide nano-particle which is prepared by the following steps: by taking a dendritic polymer PAMAM as a carrier, coupling a TREM-1 inhibitory peptide with amino groups on the carrier by utilizing the characteristic of polyamino groups on the surface of the PAMAM, and coupling a brain-targeted peptide with the other part of amino groups on the carrier through MAL-PEG2000-NHS, so as to obtain the TREM-1 inhibitory peptide nano-particle. And modifying the residual amino groups on the carrier with PEG (Polyethylene Glycol) to obtain the nano-particles. The nanoparticles can relieve encephaledema, neuroinflammation injury and blood brain barrier damage caused by traumatic craniocerebral injury, improve cognitive impairment and long-term prognosis after trauma, and have a very high curative effect on clinical treatment of craniocerebral injury.

The invention discloses a tumor targeting reagent and application thereof in tumor diagnosis. The targeting reagent has strong affinity to tumors, strong specificity and no biotoxicity, is relatively stable in vivo, and can be used as a molecular probe to be applied to molecular imaging and / or treatment of various integrin alpha6 high-expression tumors; the targeting reagent provided by the invention comprises the tumor targeting peptide RWYDeA, and further comprises a radionuclide chelating agent, other connecting structures and a hydroxyl substitution structure, and the structure is beneficial to improving the tumor targeting property of the targeting reagent, prolonging the tumor binding time and achieving a better tumor diagnosis effect; the method can be used for preparing products for diagnosing / treating diseases related to high expression of integrin alpha6.

The invention discloses an ROS-responsive prostate cancer targeting prodrug as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The prodrug is formed by covalently linking a prostate cancer targeting peptide SYEELR, a ROS (reactive oxygen species) response type ketal thiol (TK) connexon and an active molecule Devimistat. The preparation method comprises the following two steps: firstly, coupling Devimistat with a TK linker to obtain an intermediate Dev-TK, and then coupling the intermediate Dev-TK with an SYEELR peptide fragment to obtain a target product. The prodrug can specifically break and release Devimistat in a tumor high active oxygen microenvironment, and active targeting delivery is realized by virtue of the SYEELR peptide. The invention provides a novel prostatic cancer treatment strategy with accurate targeting, controllable release, enhanced curative effect and toxicity reduction potential.

The invention relates to the technical field of food processing, and particularly discloses soybean flavor peptide and a preparation method and application thereof. Aspergillus oryzae is selected as a leavening agent, a microbial endogenous enzyme generated by full fermentation of the aspergillus oryzae is utilized to cooperate with a compound enzyme preparation to perform combined action, abundant enzyme systems effectively perform enzymolysis on macromolecules such as protein, and related taste substances are provided for subsequent enzymolysis products; in the soybean flavor peptide prepared by enzyme hydrolysis and Maillard reaction, the contents of target peptide and target amino acid (umami peptide, umami amino acid and the like) are high; when the soybean flavor peptide is applied to the compound seasoning with the main function of increasing freshness, the freshness score can be increased by 0.5-2 times. In addition, on the basis of improving freshness, the taste richness of the food or the compound seasoning can be enhanced, and the aftertaste time is prolonged.

The invention provides a cyclodextrin-based nano anti-tumor medicine preparation as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: preparing a graphene quantum dot deposition gold nanocage modified by a silane coupling agent, carrying out a Schiff base reaction on the graphene quantum dot deposition gold nanocage and aldehyde substituted-beta-cyclodextrin to prepare a composite carrier, and coating a targeted peptide-drug compound through the reaction, thereby preparing the cyclodextrin-based nano anti-tumor drug preparation. According to the cyclodextrin-based nano anti-tumor medicine preparation prepared by the invention, the anti-tumor medicine effect is obviously improved, meanwhile, the medicine resistance of the medicine can be reversed, the damage to normal tissues is reduced, the use amount of the medicine is reduced, the side effect after the medicine is used is reduced, the compliance of a patient is improved, and meanwhile, the stability and biocompatibility of the medicine can be improved; wide application prospects are realized.

The invention provides a synthesis method of tilpotide, which comprises the following steps: dividing a target peptide chain into six fragments, and adopting bis (4-C18-C28 alkoxy phenyl) methylamine, 2, 4-bis (18-C28 alkoxy)-benzyl alcohol and NH2-Asp (OTAG)-OR1 as hydrophobic label carriers to assist synthesis. A side chain carboxyl group of aspartic acid is innovatively used as an anchoring position of a hydrophobic label, a new fragment connection route is designed, a full-protection tilpotide conjugate is synthesized through a multi-label synergistic effect, and finally a protecting group and a label are removed by using a TFA lysate. Compared with a solid-phase synthesis method, the method is carried out in a homogeneous reaction, the dosage of amino acid and the condensing agent is reduced to 1-1.2 times from 2-3 times, and the cost is remarkably reduced. Compared with an existing hydrophobic labeling method, the method has the advantages that multiple labels can be introduced to serve as protecting groups, the yield and purity of synthesis of medium-chain and long-chain polypeptides are improved, meanwhile, the problem of hydrophobicity attenuation caused by peptide chain extension is solved, and the green chemical process requirement is met.

The invention discloses a muscle-bone regeneration sustained-release preparation for targeting knee joint cartilage and a preparation process thereof, the preparation is composed of a cartilage targeting peptide modified PLGA-hyaluronic acid composite nanoparticle sustained-release carrier, an active regeneration component, a biocompatible matrix and an anti-inflammatory component, nanoparticles are prepared by a multiple emulsion solvent evaporation method, and targeting peptide is modified; the composite concentrated growth factors, stem cells and traditional Chinese medicine extracts have the functions of targeted enrichment, long-acting slow release, anti-inflammation and cartilage regeneration promotion, and can be used for knee joint cartilage defect repair.

The invention discloses a prebiotic-loaded intestinal targeting exosome and a preparation method and application thereof.The preparation method comprises the steps that firstly, plasmids containing intestinal cell targeting peptide sequences are constructed through a gene editing technology, the plasmids are transfected and introduced into cells, the transfected cells are screened with antibiotics, and cell strains stably expressing intestinal cell targeting peptides are obtained; then culturing and adding prebiotics, and continuously culturing to enable the cells to take in the prebiotics and release the exosomes loaded with the prebiotics; and finally, collecting cell culture supernate, and centrifuging and resuspending for multiple times under a low-temperature condition to obtain the prebiotics-loaded intestinal targeting exosome. According to the preparation method, extraction of the exosome, loading of the prebiotics and an intestinal targeting modification process are optimized, so that the prepared intestinal targeting exosome loaded with the prebiotics can be used for remarkably improving the loading efficiency and intestinal targeting of the prebiotics and enhancing the stability of the exosome in gastrointestinal tracts; therefore, the delivery efficiency and the treatment effect of the prebiotics in the intestinal tract are improved.

The invention discloses a preparation method and application of a magnetic resonance imaging nanoprobe capable of penetrating pancreatic cancer tumor interstitial substance, and belongs to the technical field of self-driven magnetic resonance imaging nanoprobes. The magnetic resonance imaging nanoprobe Ag / Ag2S-GP capable of penetrating pancreatic cancer tumor interstitial substance can be subjected to oxidation-reduction reaction with H2O2 in a tumor microenvironment to continuously generate oxygen airflow, and the reverse thrust of the airflow autonomously drives the probe to move towards the deep part of the tumor interstitial substance; in addition, the Plectin-1 targeting peptide with the Ag / Ag2S-GP surface modified can also be specifically combined with Plectin-1 protein highly expressed by pancreatic cancer cells, and accurate spatial positioning and monitoring of pancreatic cancer are achieved under the guidance of MRI (Magnetic Resonance Imaging). Meanwhile, Ag / Ag2S-GP reduces Schottky barriers under the piezoelectric catalysis action of ultrasonic excitation, hydroxyl radicals are generated, tumor cell apoptosis is induced, and therefore the pancreatic cancer diagnosis and treatment effect is enhanced.

The invention particularly relates to a ginseng peptide with anti-aging activity and a preparation method and application thereof, and belongs to the technical field of bioactive peptides. The method comprises the following steps: by taking ginseng fibrous roots, ginseng leaves and other by-products as raw materials, carrying out weak acid ultrasonic pretreatment, loading natural anti-aging active components, carrying out low-temperature high-pressure homogenization and airflow crushing synergistic treatment, carrying out double-stage composite enzymolysis, clarifying with an alternating electric field ceramic membrane, carrying out five-stage series ultrafiltration classification, carrying out four-stage filter element sterilization, freeze-drying and the like in sequence. The high-purity ginseng peptide is prepared. The total nitrogen content of the prepared ginseng peptide is larger than or equal to 14.8%, the proportion of a target peptide fragment is larger than or equal to 97.2%, the yield is larger than or equal to 18.5%, and the ginseng peptide has good antioxidant and anti-aging activity and can be used for development of functional food and skin care products. The method is low in cost, high in resource utilization rate and green and efficient in process, and the product purity and activity are remarkably superior to those of a traditional method.

The invention relates to the technical field of giant salamander peptide separation and purification, in particular to a giant salamander peptide extraction and separation and purification method and system.The method comprises the steps that healthy giant salamanders and giant salamander processing equipment are determined, giant salamander processing by-products are obtained, the giant salamander processing by-products are pretreated, to-be-processed by-products are obtained, the to-be-processed by-products are degreased and deodorized, and the giant salamander peptide is obtained. Preparing giant salamander protein dry powder, preparing giant salamander crude peptide liquid, obtaining a target peptide fragment, finely separating the target peptide fragment to obtain target active peptide distillate, freeze-drying the target active peptide distillate to obtain high-purity giant salamander peptide powder, and storing the molecular weight, amino acid sequence and activity verification parameters of the peptide fragment. And extracting, separating and purifying the giant salamander peptide based on the optimized storage database and the giant salamander grease. The extraction efficiency and the separation and purification efficiency of the giant salamander peptide can be improved.

The application provides a thermoelectric hydrogel composite dressing for promoting repair of diabetic ulcers and loading of targeted modified exosomes and a preparation method, which comprises the following steps: preparing methacrylated hyaluronic acid; preparing polyether F127 diacrylate; co-incubating exosomes with palmitic acid grafted targeting peptides to prepare targeted modified exosomes; mixing the methacrylated hyaluronic acid, the polyether F127 diacrylate, a photosensitizer, the targeted modified exosomes and an electrolyte, and crosslinking under ultraviolet light to form a composite gel. The composite dressing is a novel composite dressing for thermoelectric stimulation and exosome treatment. On one hand, the dressing generates a wireless and passive electric field to simulate the endogenous electric field in the wound, promote directional migration of skin cells, accelerate reepithelialization and dermal formation; on the other hand, the dressing releases exosomes to precisely target blood vessels of a wound lesion, reverse endothelial dysfunction in a high-sugar environment and promote angiogenesis, and effectively solves the problems in repair and regeneration of diabetic ulcer wounds.

The invention provides an anti-sugar and anti-aging cod collagen peptide as well as a preparation method and application thereof, according to the method, the yield and functional characteristics of active peptide are remarkably improved by combining ultrasonic wave with nanometer grinding pretreatment and combining step-by-step directional enzymolysis and a three-stage ultrafiltration membrane precise separation technology. The method specifically comprises the following steps: carrying out ultrasonic pretreatment on dry cod skin, and then soaking and activating in warm water containing natural anti-sugar components; grinding and dissociating collagenous fibers by adopting high-pressure homogenization and nanoscale zirconium oxide beads; the high-purity peptide fragment with the molecular weight of 500-3000Da is obtained through step-by-step enzymolysis of a compound enzyme I and an enzyme II in combination with gradient interception of a three-stage polyethersulfone ultrafiltration membrane. The total nitrogen content of the obtained product is larger than or equal to 14.5%, the proportion of the target peptide fragment is larger than or equal to 96.7%, the yield is larger than or equal to 18.1%, and the product has remarkable anti-saccharification and anti-aging activity and can be applied to skin care products such as essence and masks to achieve collagen repair and skin barrier function improvement.

The invention discloses a method and a device for reversely screening special protease based on a target active peptide, which are applied to the field of bioactive peptides, and are characterized in that a target peptide database and a negative peptide database are established based on task requirements, so that expected active peptides and peptides with adverse effects can be clearly distinguished in the screening process, thereby ensuring that the target is clear and the screening efficiency is high. And virtual enzyme digestion is carried out on the target protein data based on a preset enzyme digestion rule to obtain a first theoretical enzyme digestion peptide fragment and corresponding target peptide information, so that the applicability of each protease can be accurately evaluated directly according to the quantity and quality of the target peptide generated by each protease in the screening process, and the screening efficiency is improved. Therefore, the accuracy of the screening process is improved; the candidate protease set is further screened in combination with the negative peptide information, and the enzyme generating the negative peptide is excluded, so that the screened protease can preferentially generate the target peptide, adverse byproducts are avoided, and the screening precision of the protease is improved.

The disclosure relates to compositions, methods, and processes for the preparation, use, and / or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue.

The invention discloses an Alzheimer's disease transnasal microneedle drug delivery system and a preparation method thereof, and relates to the technical field of biological medicine, the microneedle drug delivery system comprises a microneedle substrate and a microneedle tip; the microneedle tip comprises a medicine carrying exosome, hyaluronic acid and polyvinylpyrrolidone; the drug-loaded exosome comprises a tobacco leaf exosome, the surface of the tobacco leaf exosome is connected with brain-targeted peptide RVG29, and donepezil is wrapped in the tobacco leaf exosome. The invention further discloses a preparation method of the microneedle drug delivery system. According to the nasal microneedle drug delivery system for Alzheimer's disease, the delivery efficiency of a small molecule drug donepezil to the brain is enhanced, and the nasal microneedle drug delivery system has the advantages of being high in safety, good in curative effect and good in targeting property; the problems that in the prior art, small molecule medicine donepezil difficultly passes through the blood brain barrier and enters the central nervous system, the medicine delivery efficiency is low, the treatment effect is low, and side effects are many are solved.

The present invention provides a means to target pharmaceutical agents to sites of inflammation within the body using a conjugate that includes the pharmaceutical agent linked by a cleavable linker to a polymer. The conjugate may also include a homing molecule, such as the targeting peptide CRV, linked to the polymer via a second linker. Specifically, the present invention provides the conjugates and methods of using these conjugates to reduce inflammation and treat inflammatory diseases.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

Among other things, the present disclosure provides antibodies and antigen-binding fragments thereof, conjugates, compositions, and methods thereof that can be used for various purposes, including treating conditions, disorders, or diseases including cancer, e.g., prostate cancer. In some embodiments, antibodies and antigen-binding fragments thereof comprise one or more bone-targeting moieties, e.g., one or more bone-targeting peptides. In some embodiments, antibodies and antigen-binding fragments thereof, conjugates, compositions, and methods thereof can be used to treat bone metastases.

The disclosure relates to compositions, methods, and processes for the preparation, use, and / or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue.

The invention relates to an SR-B1 targeted polypeptide compound and application thereof in preparation of antitumor drugs, and belongs to the technical field of biological drug manufacturing. In order to solve the problems that the existing PDT photosensitizer is insufficient in targeting property and the treatment effect is limited due to the action of intracellular cholesterol, the invention provides an SR-B1 targeting polypeptide compound which is composed of an SR-B1 targeting polypeptide and an AS1411 aptamer. The SR-B1 targeting polypeptide comprises an SR-B1 targeting peptide fragment, a self-assembly peptide fragment, a response enzyme digestion peptide fragment and a photosensitizer TCPP which are connected in sequence. The invention constructs a polypeptide targeting system based on a dual self-assembly strategy, which not only can target SR-B1, inhibit a cholesterol-mediated tumor for a long time to promote a signal channel and interfere the ROS steady state, but also can target TCPP to a cell nucleus, improve the photosensitivity of PDT, and further improve the killing effect on tumor cells by improving the yield of ROS to cause the damage to the cell nucleus.

The invention belongs to the technical field of targeting peptides, and particularly relates to an EGFR targeting peptide, a fluorescent probe, a composition and application. The targeting peptide has an amino acid sequence as shown in SEQ ID NO: 1. Through verification, the targeting peptide has the capability of selective enrichment in tumor cells, and is beneficial to tumor recognition and treatment. The fluorescent probe coupled by the targeting peptide and the ICG fluorescent dye can specifically recognize and target an EGFR receptor, realizes effective aggregation and retention at a tumor part, is suitable for targeted diagnosis and treatment of tumors, can realize accurate imaging, has important clinical transformation potential, and becomes a molecular imaging probe with application prospects.

The invention belongs to the technical field of biological medicine, and provides self-assembled polypeptide targeting mitochondria, a preparation method and application of the self-assembled polypeptide, a self-assembled polypeptide microneedle patch and a preparation method of the self-assembled polypeptide microneedle patch. According to the self-assembly polypeptide of the targeted mitochondria, the amino acid sequence is GFFGKXRXKXRX, and X represents cyclohexyl alanine. A targeting peptide (Mito) sequence KXRXKXRX can recognize cell mitochondria in a targeting manner, a self-assembly sequence GFFG is introduced to an N terminal of the Mito sequence KXRXKXRX, and the targeting peptide sequence is endowed with a self-assembly capability, so that the self-assembly polypeptide can cause morphological function abnormality of mitochondria in tumor cells, the tumor cells are killed, and inhibition of the tumor cells is realized. The self-assembled polypeptide disclosed by the invention is applied to treatment of tumor cells through in-situ administration of the self-assembled polypeptide microneedle patch. Oral squamous cell carcinoma serves as a highly proliferated tumor cell, rapid proliferation of the oral squamous cell carcinoma needs a large amount of energy supply for mitochondria, and therefore attacking of the mitochondria can serve as an effective treatment target.

The invention discloses mucosal immune-enhanced recombinant lactobacillus for expressing PEDV (porcine epidemic diarrhea virus) S1 protein as well as a construction method and application of the mucosal immune-enhanced recombinant lactobacillus. The mucosal immune-enhanced recombinant lactobacillus contains a recombinant lactobacillus expression vector for expressing PEDV S1 protein, the amino acid of the PEDV S1 protein is fused with M cell targeting peptide (Co1) and dendritic cell targeting peptide (6aa), the carboxyl terminal of the PEDV S1 protein is fused with a mucosal immune adjuvant (LTB), and the connection sequence of all the parts is Co1-6aa-S1-LTB. According to the mucosal immune enhanced recombinant lactobacillus constructed by the invention, through the synergistic effect of the targeting peptide (DC / MC targeting peptide) and the adjuvant (LTB), the mucosal immune response efficiency (including intestinal mucus SIgA and serum IgG levels) of a PEDV S1 antigen is remarkably improved, humoral immunity, cellular immunity and mucosal immune responses of pregnant animals can be remarkably induced, maternal antibodies are generated, and the immune response efficiency of the pregnant animals is remarkably improved. The intestinal SIgA level of newborn animals is improved, and an effective technical means is provided for prevention and treatment of PED.