51 results about "Transit Peptide" patented technology

The present invention relates a method for transforming a C3 plant to provide it with a C4 photoshynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a C3 plant.

An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position −72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.

The invention discloses a method for locating and expressing foreign protein in mitochondria, belongs to the field of molecular genetics, provides a DNA sequence for encoding polypeptides, applicationof the DNA sequence in location and expressing of the foreign protein in the mitochondria and a related approach, and particularly relates to a sequence from a phyllostachys edulis genome. A polypeptide encoded by the sequence can express the foreign protein in the mitochondria, serve as a marker gene for co-location of the mitochondria and provide a reliable comparison approach for subcell location. The method comprises the steps of firstly, cloning a sequence of a phyllostachys edulis gene, and introducing yellow fluorescent protein connected to a C end of the sequence into the mitochondria. Transfer peptides and the fluorescent protein are fused, observation can be carried out in living cells, time and labor are saved, and the method is a basic method for researching the functions of the protein.