45 results about "Transit Peptide" patented technology

Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyandenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localized enzyme and then a second transit peptide of a plant gene encoding, a plastid-localized enzyme. Production of glyphosate-tolerant plants is disclosed.

The present invention discloses methods and materials for delivering a cargo compound into a brain cancer cell and/or across the blood-brain barrier. Delivery of the cargo compound is accomplished by the use of protein transport peptides derived from Neisseria outer membrane proteins, such as Laz. The invention also provides synthetic transit peptides comprised of the pentapeptide AAEAP. The invention further discloses methods for treating cancer, and specifically brain cancer, as well as other brain-related conditions. Further, the invention provides methods of imaging and diagnosing cancer, particular brain cancer.

The present invention relates a method for transforming a rice plant to provide it with a C4 photosynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a rice plant.

The invention describes isolated transport peptides, which cross the cell membrane of a cell and/or home to a target cell. The invention also describes a transport complex in which a transport peptide is linked to a cargo moiety to be delivered into/to a cell. Methods are disclosed describing delivery of a transport complex into and/or to a cell. Vectors and host cells comprising transport peptides and transport complexes are also described, as well as pharmaceutical compositions including transport complexes of the present invention.

The object of the invention is to provide an EGFR-derived peptide useful for EGFR-based immunotherapy.

The invention provides an EGFR-derived peptide capable of inducing both cellular and humoral immune responses and mutant peptide thereof and a polypeptide comprising said peptide, a nucleic acid molecule encoding the same, and a pharmaceutical composition comprising the same.

An chimerical polypeptide that arrests proliferating cells in mitosis is provided. In general the polypeptide has an N-terminal transit peptide, such as HIV-1 Tat, and a C-terminal cell-cycle effector, such as a G2/M cyclin or a cytostatic factor. A polynucleotide under the control of a heterologous promoter that encodes a polypeptide that arrests cells in mitosis is also provided. The polynucleotide may, for example, encode a G2/M cyclin. Pharmaceutical compositions comprising the agent that inhibits transit through mitosis is provided. A method of treating patients suffering from a hyperplasia, such as cancer, psoriasis or benign prostate hyperplasia is provided.

The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying modulators of the transporter peptides.

An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position−72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.

The present invention relates to a recombinant nucleic acid encoding a hybrid polypeptide which comprises a transit peptide for the translocation of the polypeptide into an appropriate organelles such as plastids, a starch-encapsulating region from maize starch synthase and a payload polypeptide, wherein said payload polypeptide can be either N- or C-terminal to the starch encapsulating region. The invention also relates to the expression vectors comprising said nucleic acid, and hosts comprising the said vector. Also, the invention encompasses methods of producing the hybrid polypeptide thereof from starch and particularly from starch granules, and industrial uses of the payload polypeptide recombinantly produced in said hybrid polypeptide wherein said payload polypeptide is a biologically active molecule.

The invention discloses a plant expression vector and the application thereof in preparing phosphorylation modified rice starch. The plant expression vector comprises a target gene inserting into an original vector and a promoter, wherein the target gene comprises a transit peptides gene segment of granule-bound starch synthase and a potato glucan-water dikinase gene which are sequentially connected, and the promoter is a barley endosperm specific promoter HorD. For the plant expression vector and the application thereof in preparing phosphorylation modified rice starch, the potato glucan-water dikinase gene is transferred into rice, and the starch of the rice can be phosphorylated through the expression of the potato glucan-water dikinase gene, so that the modification of the starch is realized. The potato glucan-water dikinase is led to amyloid through the transit peptides of the granule-bound starch synthase, and phosphorylation modification is performed on the starch. According to the invention, the expression of the potato glucan-water dikinase gene in the rice endosperm can be promoted through the barley endosperm specific promoter HorD, and the phosphorus content in the rice starch is greatly increased.

The present invention concerns a transformed microalga producing a protein harboring a “high mannose” pattern of glycosylation in the plastid of the transformed microalga, wherein 1) the transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) the microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, the nucleic acid sequence encoding an amino acid sequence including (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of the protein, 3) the xylosyltransferases and fucosyltransferases of the microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of the microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of the microalga have not been inactivated.

Methods and compositions comprising an expression construct and a suppressor of posttranscriptional gene silencing construct are described. The expression construct and suppressor construct may comprise a viral amplicon. The expression construct may comprise fusing the target gene to the 3′ and/or 5′ end of a gene encoding a transit peptide sequence or a signaling peptide sequences. The transit or signal peptide sequence directs the target gene product to a subcellular location. Methods comprise the production of several heterologous proteins in a single plant. The invention comprises methods for plant production and protein harvest that will yield useful amounts of the desired protein(s) in as little as one to two weeks after the initiation of the production cycle. Methods for the inoculation of recipient plants by spraying with recombinant Agrobacterium suspensions containing the constructs of interest are taught.

The present invention discloses pharmaceutical compositions and methods for using a fusion protein comprising a superoxide dismutase and a transit peptide. The present invention also discloses pharmaceutical compositions and methods for using the fusion protein in combination with other antiretroviral agents for treating patients with AIDS or HTV infection.

Methods for improving the efficiency of photosynthesis in plants exposed to suboptimal light conditions. Photosynthesis enhancement is achieved by transformation and expression of one or more exogenous chromophores in the chloroplast of plants or in the cytoplasm under the control of a transit peptide which directs it to the chloroplast or a compartment within the chloroplast. Preferred chromophores have excitation max in the green-yellow light spectrum. Chains of chromophores can be used to capture and emit light from one to the other until the emitted wave length is in the range that can be efficiently utilize by the native light harvest complex.