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Compositions and methods for enhancing plant photosynthetic activity

A photosynthetic activity, plant technology, applied in the field of such plants with enhanced photosynthetic capacity, can solve the problems of inefficiently driving photosynthesis, unable to drive photosynthesis, etc.

Inactive Publication Date: 2015-07-08
FUTURAGENE ISRAEL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Part of the light spectrum is therefore not available to drive photosynthesis or drives photosynthesis inefficiently

Method used

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  • Compositions and methods for enhancing plant photosynthetic activity
  • Compositions and methods for enhancing plant photosynthetic activity
  • Compositions and methods for enhancing plant photosynthetic activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Example 1: Construct Preparation and Plant Transformation

[0097] One or more constructs comprising chromophores that individually or collectively absorb light and emit light that can be used to drive photosynthesis are constructed and transformed into plants, and such transformants are isolated.

Embodiment 2

[0098] Example 2: Characterization of Fluorescence in Transgenic Plant Leaves

[0099] Fluorescent absorption and emission of recombinant proteins were measured using fluorescence microscopy. Using a combined fluorescence microscope, i.e. Zeiss III-RS or Zeiss Axiovert 100S (Zeiss), at one or more appropriate photoexcitation wavelengths, i.e., the excitation maxima of the test transgenic chromophore(s) to be investigated , fresh leaves from transformed plants described in Example 1 and untransformed controls were studied. The image is recorded electronically or on a film.

Embodiment 3

[0100] Example 3: Photosynthetic activity of transgenic plants

[0101] Chlorophyll fluorescence measurement

[0102] Measurements of adjusted chlorophyll fluorescence emission from the upper surface of leaves were performed using a pulse amplitude modulated fluorometer (PAM-101; H.Waltz, Effeltrich, Germany (MONOTORING-PAM chlorophyll fluorometer). Via blue power LED (blue power LED ), each probe produces adjusted fluorescence excitation light, continuous actinic light (actinic light) and saturation light (saturation flashes). Make the light source and signal detection and saturation light (saturating light) keep 5mm away from the leaf upper surface. Optical fiber Used to direct the light from the power supply and control unit to the sample, and to direct the light back from the sample. The measured intensity of the adjusted red light is ~0.1 μmol m -2 the s -1 . Leaves were dark-adapted for 10 minutes in a zero-light environment before measuring fluorescence induction. T...

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Abstract

Methods for improving the efficiency of photosynthesis in plants exposed to suboptimal light conditions. Photosynthesis enhancement is achieved by transformation and expression of one or more exogenous chromophores in the chloroplast of plants or in the cytoplasm under the control of a transit peptide which directs it to the chloroplast or a compartment within the chloroplast. Preferred chromophores have excitation max in the green-yellow light spectrum. Chains of chromophores can be used to capture and emit light from one to the other until the emitted wave length is in the range that can be efficiently utilize by the native light harvest complex.

Description

[0001] Cross References to Related Applications [0002] This application claims priority under Section 119(e) of the US Patent Act to provisional applications 61 / 656,794, filed June 7, 2012, and 61 / 672,500, filed July 17, 2012. Each of the foregoing provisional applications is hereby incorporated by reference in its entirety. [0003] sequence listing [0004] This application contains a Sequence Listing, submitted via EFS-Web in ASCII format, which is hereby incorporated by reference in its entirety. Said ASCII copy, created on June 7, 2013, is named 30407-0005WO1_SL.txt and is 118,885 bytes in size. technical field [0005] The present invention relates to transgenic plants with enhanced photosynthetic performance, and more particularly to such plants with enhanced photosynthetic capacity under low light conditions. Background technique [0006] Photosynthetic plants rely on light, eg, sunlight, as their energy source. It is well established in agricultural cultivatio...

Claims

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Application Information

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IPC IPC(8): C12N15/82
CPCC12N15/8269A01G1/001A01C14/00A01H5/10C12N15/8262A01G22/00
Inventor D·阿维萨尔M·艾布拉姆森D·西格尔Z·萨尼S·赫希
Owner FUTURAGENE ISRAEL
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