50 results about "Fluorometer" patented technology

The invention belongs to the technical field of intelligent irrigation, and discloses a garden environment monitoring and management system based on the Internet of Things technology, and the system comprises a soil moisture monitoring module which obtains the moisture content of a root layer of garden vegetation through an implantable TDR soil moisture sensor array; the carbon flux sensing module is used for respectively monitoring carbon dioxide flux and vegetation photosynthetic efficiency through a vortex covariance tower and a chlorophyll luminoscope and acquiring carbon flux data and photosynthetic efficiency parameters; obtaining net carbon exchange capacity based on the carbon flux data and the photosynthetic efficiency parameters; the data coupling module is used for performing coupling analysis on the moisture content of the root system layer and the net carbon exchange capacity, establishing a moisture-carbon fixed correlation model, calculating a carbon sink efficiency index and judging whether an initial irrigation demand signal is generated or not; the utilization rate of water resources is increased; and meanwhile, ecological benefits are considered.

The invention relates to the technical field of biomedical analysis instruments, and discloses a DNA quantitative fluorometer calibration system based on artificial intelligence optimization, which comprises a data acquisition module for acquiring a dynamic fluorescence characteristic spectrum for representing the whole process of fluorescence reaction; and an intelligent calibration and diagnosis core model in the data processing unit analyzes the spectrogram by using a long short-term memory network and an attention mechanism so as to generate a multi-dimensional diagnosis result containing predicted concentration, confidence score and interference early warning. According to the method, the dynamic fluorescence characteristic spectrum of the standard substance is used as a scene anchor point, the preset global basic model is finely adjusted, and the special session model is generated, so that the real-time self-adaptive calibration of the change of the instrument and the reagent is realized. According to the method, the defects that a traditional method depends on an end point value, is easily interfered and is static in calibration are overcome, and the accuracy, reliability and long-term stability of a DNA quantitative result are remarkably improved.

A water quality analysis system is calibrated without using any organic fluorescence material such as phenanthrene. The water quality analysis system analyzes a component to be measured contained in a liquid sample by a fluorescence spectroscopy, and that comprises a light irradiation section that irradiates the liquid sample with a light having an excitation wavelength of the component to be measured, a light detection section that detects a fluorescence of the component to be measured emitted from the liquid sample, a calculation unit that calculates a concentration of the component to be measured by using a fluorescence intensity obtained by the light detection section, and a solid fluorescence reference member that is arranged on an optical path between the light irradiation section and the light detection section and that emits the fluorescence by the light from the light irradiation section at a time of calibration.

A fluorimeter reference cell which comprises a first material which emits an absorption spectrum when an excitation light is incident upon the first material. The absorption spectrum has a defined cha

A method for measuring drug content indexes of a non-viral nucleic acid vector composition, comprising performing labeling by enabling a permeable nucleic acid dye to be in contact with a non-viral nucleic acid vector (for example, an mRNA-LNP). The drug content indexes are calculated by measuring, at a single-particle level, fluorescent light generated by free nucleic acids of a nucleic acid vector, and fluorescent light generated by externally adsorbed nucleic acids and internally entrapped nucleic acids of the non-viral nucleic acid vector. The drug content indexes include a drug loading capacity, entrapment efficiency and the like. The method optionally further comprises: treating a sample with a nuclease before or after performing labeling by using the permeable nucleic acid dye, and when performing treatment with the nuclease, measuring the fluorescence condition of the non-viral nucleic acid vector composition; or enabling the permeable nucleic acid dye to be in contact with the sample, and then measuring the fluorescence condition of the non-viral nucleic acid vector composition.

The invention discloses a thermal barrier coating temperature and stress coupling test system and method, and belongs to the technical field of temperature and stress non-contact detection. The system comprises an exciting light system, an exciting light transmission system, a phosphorescence / fluorescence light splitting light path and acquisition system and a data processing system, the exciting light system is used for emitting 355 nm and 532 nm laser, and the exciting light transmission system is used for inputting the 355 nm and 532 nm laser to a thermal barrier coating of a sample. The phosphorescence / fluorescence light splitting light path and acquisition system is used for splitting light signals emitted by the thermal barrier coating through excitation, dividing the light signals into phosphorescence and fluorescence and performing acquisition, and the data processing system is used for calculating a temperature field and a stress field of the thermal barrier coating based on the acquired phosphorescence and fluorescence. According to the invention, the problem that the temperature and stress coupling effect of the thermal barrier coating interferes with each other in the test is solved, and the problem that the temperature and stress of the thermal barrier coating cannot be tested in a real-time, in-situ, collaborative and non-contact manner in the prior art is solved.

The invention relates to the field of protein detection, in particular to a method for detecting phosphorylated Tau-217 protein in urine of an Alzheimer's disease patient, which comprises the following steps of: 1, collecting fresh urine in the morning by using a urine collecting container, and 2, taking out 100 microliters of urine by using a transfer pipette and adding the urine into a 5.0 ml centrifugal tube; step 3, adding 4.9 ml of a urine diluent to dilute the urine; and step 4, prefabricating tracing fluorescent microspheres and enrichment microspheres in the centrifugal tube. Step 5, repeatedly reversing and uniformly mixing, and reacting at room temperature for 8-12 hours; step 5, placing the reaction tube on a separation test tube rack with a magnet for more than 20 minutes; step 6, irradiating the test tube by using a highlighter with the wavelength of about 340nm in a dark environment to judge a result; and step 7, if a red fluorescent dot appears near the magnet in the test tube, the result is positive, otherwise, the result is negative, and the result can also be judged by measuring a fluorescence value through a luminoscope with the wavelength of 340nm. Finally, the phosphorylated Tau-217 protein is detected, and the operation threshold and the economic cost are reduced.

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

The instrument for processing microscopic particles may include a combination of modules integrated into a functional system. The system may be composed of synchronized subsystems including pneumatic modules, fluorometers, multi-wavelength lasers, dual view microscopy, high-voltage generators and other subsystems designed to analyze and control microfluidic processes occurring on a chip. The system performs the combined functions of microfluidic particle generation, analysis, and selection, making it possible for the user to create custom integrated workflows for molecular and cell biology processing. Creating the integrated platform requires an innovative and multi-disciplinary approach for module mechanical integration, electronic synchronization and user interface design for controlling diverse microfluidic processes at high speed.

The application discloses a sequencing system and a bearing device. The sequencing system comprises a bearing device, a fluid device, an imaging device and a computing device. The bearing device is used for bearing and adjusting the temperature of a reactor; the fluid device is connected with the bearing device and is used for controllably moving one or more reagents with fluorescent labels into contact with polynucleotides; the imaging device is located above the bearing device and is used for exciting and collecting fluorescence generated by the fluorescent labels; and the computing device is operatively coupled with the imaging device and is used for acquiring an instruction set of the fluorescence signal from the imaging device. The sequencing system in the application can better realize focusing and focus pursuit to meet imaging requirements during sequencing, can better control the temperature of a reaction environment to ensure effective biochemical reactions, and can make related parts have smaller deformation and / or maintain a relatively stable connection relationship during temperature adjustment through reasonable layout of mechanical hardware, so that the sequencing accuracy and the service life of the instrument are improved.

A vector microwave detection device comprises a detection mechanism which is provided with a microwave plate for transmitting to-be-measured microwaves and an NV color center probe, and the detection mechanism is configured to enable the NV color center probe to move along a preset path in a microwave field of the to-be-measured vector microwaves; the microscope lens is mounted above the detection mechanism, is suitable for focusing the laser onto the NV color center probe so as to enable the NV color center to emit fluorescence under the action of the laser, and is suitable for collecting the fluorescence emitted by the NV color center under the action of the laser; the counting mechanism is suitable for counting the fluorescence collected by the microscope lens; and the calculation mechanism is suitable for obtaining the polarization direction of the vector microwave field to be measured according to the projection relationship between the first and second Labir oscillation frequencies of the NV color center probe in each strong effective field and the vector microwave to be measured in the direction of each strong effective field.

A system for analyzing deposits (e.g., scale, siltation, biofilm, or corrosion) within a fluid treatment system 10, such as an industrial water system 12, is described. The system includes a conduit 16 adapted to be fluidically coupled to the fluid treatment system to receive a fluid stream from the fluid treatment system; a substrate 18 within the conduit, the substrate having a surface representative of a surface within the fluid treatment system; a device 40 for varying the temperature of the substrate; at least one temperature sensor 44, 46, 48, 50 for measuring heat transfer through the substrate; at least one fluorometer 54 for monitoring fluorescence of the substrate surface caused by microorganisms at a plurality of fluorometer positions; at least one camera 56 for acquiring images of the substrate surface at a plurality of camera positions; and a controller for receiving data from the at least one temperature sensor, the at least one fluorometer, and the at least one camera and determining, based on the received data, a resistance to heat transfer through the substrate and a nature and level of deposits.

1. Name of the product in this design: Fluorometer. 2. Purpose of this design: This design is used for nucleic acid or protein quantification. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

Consumables for multi-channel liquid dispensing devices and multi-channel testing devices and use workflows of the devices are described. Consumables of the present disclosure may include multiple containers pre-loaded with reagents (e.g., known diluents of any reagents, such as standards, proteins, chemicals, or other desired materials). A multi-channel liquid dispensing device may be used to synchronously draw quantitative reagents from all containers of a consumable, and in turn to synchronously dispense the reagents into multiple rows of pores of a porous device, such as a microplate, for further downstream reaction and / or detection use. Downstream detection may be performed in a detection instrument, such as a spectrophotometer, fluorometer, or other instrument. The consumables of the present disclosure provide a variety of advantages, such as time savings as compared to preparing reagent diluents individually, measuring reagent / reagent diluents individually, and dispensing them into each individual well of a porous device for downstream reactions / assays. The consumables of the present disclosure may include a plurality of rows, each row including a consumable with a plurality of containers or cuvettes.

The utility model provides a light intensity limiting block and a fluorescence intensity measuring assembly, and belongs to the technical field of fluorescence intensity measurement, the light intensity limiting block is provided with a plurality of through holes, and the inner walls of the holes are made of black matte materials. The narrow and long black matte inner wall can absorb stray light, only a small amount of stray light almost parallel to the hole channels can pass through the narrow and long black matte inner wall, and the light intensity limiting block can be applied to a device needing stray light filtering and is particularly suitable for a fluorescence intensity measuring assembly of a fluorometer.

The invention discloses a calibration method of a nucleic acid quantitative fluorometer, which adopts a methylene blue aqueous solution with stable chemical property as a standard substance to replace a biological nucleic acid reagent, and verifies the optical performance of the nucleic acid quantitative fluorometer by constructing a linear response relationship of a chemical standard substance. The method comprises the following steps: preparing a methylene blue mother solution and a gradient calibration solution, and carrying out light-shielding standing and thermodynamic stabilization treatment; collecting a fluorescence signal by using a nucleic acid quantitative fluorometer to construct a standard curve; selecting a verification sample for reverse measurement, and calculating an indication error, a channel range and measurement repeatability; and finally, judging whether the calibration result is qualified or not through hierarchical logic including linear validity verification and metrological characteristic subentry verification. The problems that an existing RNA kit is high in calibration cost, poor in linearity and unstable in result are solved, and the method has the advantages of being low in cost, easy and convenient to operate, high in result confidence coefficient and the like.

Methods and system are described for normalizing and creating correction factors for measurements in a fluorometer. To ensure that measurements across multiple channels can be properly compared, some kind of calibration must be done. The same calibrant can be run across all the channels and fluorescence measured. The fluorescence of one channel can be chosen as a reference. A correction factor can be calculated for each channel and used for an extended period of time, possibly even the lifetime of the instrument.

1. The name of the design product: fluorometer. 2. The use of the design product: for fluorescence detection of samples. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

The invention discloses an on-line intelligent monitoring system and method for mass concentration and oxidation potential of harmful particulate matters. The system is characterized in that a particulate matter sampling subsystem comprises a particle size separator, a vacuum pump, a mixing pipeline and an HEPA (High Efficiency Particulate Air) filter; the particulate matter mass concentration detection subsystem comprises an optical detection chamber, a laser, a light trap, a collimating lens, a focusing lens and a photodiode; the particulate matter atomization trapping subsystem comprises an atomization trapping chamber, a buffer trapping liquid chamber and an ultrasonic nozzle atomizer; the particulate matter oxidation potential detection subsystem comprises a first stirring device, a PH sensor, a DTT chamber, a PH buffer solution chamber, a second stirring device, a first liquid pump, a DTNB chamber, an optical fiber probe and a light absorption luminoscope. According to the method, accurate sampling, particulate matter concentration measurement and quantitative detection operation of the oxidation potential are achieved through the system. According to the system and the method, the concentration of atmospheric particulates can be detected in real time by utilizing a light scattering technology, and quantitative detection of oxygen potential can be realized.

A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.

1. The name of the design product: Microbial fluorescence detector. 2. The use of the design product: For microbial detection in the process of sterile detection medium enrichment. 3. The design points of the design product: In shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The utility model provides an atomic luminoscope capable of automatically correcting light source drift. The atomic luminoscope capable of automatically correcting the drift of the light source comprises an atomic luminoscope body, wherein the atomic luminoscope body is provided with an automatic correction mechanism, four adjusting mechanisms and two fixing assemblies; the automatic correction mechanism comprises a driving motor, the driving motor is fixedly installed in the atomic luminoscope body, the output end of the driving motor is fixedly connected with a first bevel gear, one side of the first bevel gear is in meshed connection with a second bevel gear, the bottom surface of the second bevel gear is fixedly connected with a supporting column, and the supporting column is fixedly connected with a driving motor. And the bottom end of the supporting column is rotationally connected with the inner bottom face of the atomic luminoscope body, the top face of the second bevel gear is fixedly connected with a screw rod, and the top end of the screw rod is in threaded connection with an inner threaded pipe. The atomic luminoscope capable of automatically correcting the drifting of the light source has the advantage that the dimmer can be lifted and lowered according to different test samples.

A fluorimeter reference cell comprising a rare earth salt solution or other optically transparent solution with a well-defined and characteristic absorbance pattern which material emits an absorption spectrum when an excitation light is incident upon it. The absorption spectrum has a defined characteristic enabling wavelength calibration of the fluorimeter. The reference cell comprises a second material, a light scattering colloid such as colloidal silica. The dispersive properties result in the absorption spectrum being scattered or reflected at an angle to the angle of incidence of the excitation light incident on the reference cell.

The invention discloses an enzyme response near-infrared two-region fluorescence / computed tomography dual-mode imaging probe based on a gold nano-cluster and a preparation method and application of the enzyme response near-infrared two-region fluorescence / computed tomography dual-mode imaging probe. The probe comprises: a gold nanocluster capable of simultaneously providing near-infrared two-region fluorescence and computed tomography imaging signals; the polypeptide molecule is modified on the surface of the material. The polypeptide comprises a cathepsin B response sequence, biotin with a targeting function and a click reaction unit 2-cyanobenzothiazole. The probe disclosed by the invention is subjected to enzyme digestion and trigger click reaction under the action of highly expressed CTSB in liver cancer cells, so that the gold nanoclusters are subjected to cross-linked aggregation at tumor parts, the retention time of the probe is prolonged, NIR-II fluorescence and CT imaging effects are remarkably enhanced, and high-sensitivity detection on liver cancer is realized.

This invention relates to the field of plant stress diagnosis technology, and more particularly to a modulated fluorescence imager that uses blue and green light to calculate the photochemical efficiency Φ of leaf photosystem I. PSI The method described in this invention utilizes a Mini-PAM chlorophyll fluorometer to measure blue and green light excitation in tomato leaves of both the test and control groups to generate fluorescence. Based on the measurement results, the photochemical efficiency Φ of photosystem I is calculated. PSI This allows for the diagnosis of the condition of the crop under test. The photochemical efficiency Φ of the photosystem I mentioned in this invention... PSI It is more sensitive to stress and is more effective in reflecting adverse stress. It is expected to be applied to the early diagnosis of stress in plants, so as to intervene in time and reduce losses.

The invention relates to the technical field of biology, in particular to test liquid with a bridging probe and a locking probe and a detection method of the test liquid. The invention aims to solve the problems that the existing gene detection efficiency is low, the detection cost is high, and the condition that n nucleotide sequences exist in a DNA sequence and are not quantitatively repeated cannot be accurately measured. The invention relates to a test solution with double probes of a bridging probe and a locking probe and a detection method of the test solution. The method comprises the following steps: sequentially connecting and combining bridging probes end to end and covering a gene mutation DNA region to form a bridging type cluster, connecting the bridging type probe cluster and a lock-type probe end to end to form annular DNA, combining a universal primer with a middle region of the lock-type probe, carrying out rolling circle type DNA amplification at room temperature, adding a conventional SYBR green fluorescent dye and detecting an SYBR signal, and finally, recording data through a fluorometer, judging whether the patient has mutation or not according to the existence of fluorescence, or judging whether a plurality of repeated mutations exist or not according to the intensity of the fluorescence. According to the test, the result can be judged within 30-45 minutes, and the efficiency is improved.

The invention discloses a rice seedling blast disease intelligent analysis system based on remote sensing data analysis, and relates to the technical field of agricultural remote sensing and plant disease monitoring, the system comprises a physiological data acquisition module, a physiological feature extraction module and a disease severity calculation and severity grading module; wherein the physiological data acquisition module realizes in-situ non-invasive acquisition of the spectral reflectivity of rice seedling leaves, the maximum photochemical efficiency of a light system II and the water transport rate of stalks through various sensors such as a hyperspectral sensor and a chlorophyll luminoscope; the physiological feature extraction module fuses multiple parameters to calculate a leaf surface structure health index and a vascular function activity index; the disease grading module obtains an in-vivo disease load index through weighting, and outputs different disease grades in combination with a K-means clustering threshold value; the system solves the problems that traditional rice seedling blast diagnosis is high in subjectivity and hysteresis quality, and early-stage accurate recognition of diseases is achieved.