23 results about "Fluorometer" patented technology

A fluorimeter reference cell which comprises a first material which emits an absorption spectrum when an excitation light is incident upon the first material. The absorption spectrum has a defined cha

The invention discloses a thermal barrier coating temperature and stress coupling test system and method, and belongs to the technical field of temperature and stress non-contact detection. The system comprises an exciting light system, an exciting light transmission system, a phosphorescence / fluorescence light splitting light path and acquisition system and a data processing system, the exciting light system is used for emitting 355 nm and 532 nm laser, and the exciting light transmission system is used for inputting the 355 nm and 532 nm laser to a thermal barrier coating of a sample. The phosphorescence / fluorescence light splitting light path and acquisition system is used for splitting light signals emitted by the thermal barrier coating through excitation, dividing the light signals into phosphorescence and fluorescence and performing acquisition, and the data processing system is used for calculating a temperature field and a stress field of the thermal barrier coating based on the acquired phosphorescence and fluorescence. According to the invention, the problem that the temperature and stress coupling effect of the thermal barrier coating interferes with each other in the test is solved, and the problem that the temperature and stress of the thermal barrier coating cannot be tested in a real-time, in-situ, collaborative and non-contact manner in the prior art is solved.

The invention relates to the field of protein detection, in particular to a method for detecting phosphorylated Tau-217 protein in urine of an Alzheimer's disease patient, which comprises the following steps of: 1, collecting fresh urine in the morning by using a urine collecting container, and 2, taking out 100 microliters of urine by using a transfer pipette and adding the urine into a 5.0 ml centrifugal tube; step 3, adding 4.9 ml of a urine diluent to dilute the urine; and step 4, prefabricating tracing fluorescent microspheres and enrichment microspheres in the centrifugal tube. Step 5, repeatedly reversing and uniformly mixing, and reacting at room temperature for 8-12 hours; step 5, placing the reaction tube on a separation test tube rack with a magnet for more than 20 minutes; step 6, irradiating the test tube by using a highlighter with the wavelength of about 340nm in a dark environment to judge a result; and step 7, if a red fluorescent dot appears near the magnet in the test tube, the result is positive, otherwise, the result is negative, and the result can also be judged by measuring a fluorescence value through a luminoscope with the wavelength of 340nm. Finally, the phosphorylated Tau-217 protein is detected, and the operation threshold and the economic cost are reduced.

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

1. Name of the product in this design: Fluorometer. 2. Purpose of this design: This design is used for nucleic acid or protein quantification. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention discloses a calibration method of a nucleic acid quantitative fluorometer, which adopts a methylene blue aqueous solution with stable chemical property as a standard substance to replace a biological nucleic acid reagent, and verifies the optical performance of the nucleic acid quantitative fluorometer by constructing a linear response relationship of a chemical standard substance. The method comprises the following steps: preparing a methylene blue mother solution and a gradient calibration solution, and carrying out light-shielding standing and thermodynamic stabilization treatment; collecting a fluorescence signal by using a nucleic acid quantitative fluorometer to construct a standard curve; selecting a verification sample for reverse measurement, and calculating an indication error, a channel range and measurement repeatability; and finally, judging whether the calibration result is qualified or not through hierarchical logic including linear validity verification and metrological characteristic subentry verification. The problems that an existing RNA kit is high in calibration cost, poor in linearity and unstable in result are solved, and the method has the advantages of being low in cost, easy and convenient to operate, high in result confidence coefficient and the like.

Methods and system are described for normalizing and creating correction factors for measurements in a fluorometer. To ensure that measurements across multiple channels can be properly compared, some kind of calibration must be done. The same calibrant can be run across all the channels and fluorescence measured. The fluorescence of one channel can be chosen as a reference. A correction factor can be calculated for each channel and used for an extended period of time, possibly even the lifetime of the instrument.

1. The name of the design product: fluorometer. 2. The use of the design product: for fluorescence detection of samples. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

1. The name of the design product: Microbial fluorescence detector. 2. The use of the design product: For microbial detection in the process of sterile detection medium enrichment. 3. The design points of the design product: In shape. 4. The picture or photo that best indicates the design points: perspective view 1.

A fluorimeter reference cell comprising a rare earth salt solution or other optically transparent solution with a well-defined and characteristic absorbance pattern which material emits an absorption spectrum when an excitation light is incident upon it. The absorption spectrum has a defined characteristic enabling wavelength calibration of the fluorimeter. The reference cell comprises a second material, a light scattering colloid such as colloidal silica. The dispersive properties result in the absorption spectrum being scattered or reflected at an angle to the angle of incidence of the excitation light incident on the reference cell.

The invention discloses an enzyme response near-infrared two-region fluorescence / computed tomography dual-mode imaging probe based on a gold nano-cluster and a preparation method and application of the enzyme response near-infrared two-region fluorescence / computed tomography dual-mode imaging probe. The probe comprises: a gold nanocluster capable of simultaneously providing near-infrared two-region fluorescence and computed tomography imaging signals; the polypeptide molecule is modified on the surface of the material. The polypeptide comprises a cathepsin B response sequence, biotin with a targeting function and a click reaction unit 2-cyanobenzothiazole. The probe disclosed by the invention is subjected to enzyme digestion and trigger click reaction under the action of highly expressed CTSB in liver cancer cells, so that the gold nanoclusters are subjected to cross-linked aggregation at tumor parts, the retention time of the probe is prolonged, NIR-II fluorescence and CT imaging effects are remarkably enhanced, and high-sensitivity detection on liver cancer is realized.

This invention relates to the field of plant stress diagnosis technology, and more particularly to a modulated fluorescence imager that uses blue and green light to calculate the photochemical efficiency Φ of leaf photosystem I. PSI The method described in this invention utilizes a Mini-PAM chlorophyll fluorometer to measure blue and green light excitation in tomato leaves of both the test and control groups to generate fluorescence. Based on the measurement results, the photochemical efficiency Φ of photosystem I is calculated. PSI This allows for the diagnosis of the condition of the crop under test. The photochemical efficiency Φ of the photosystem I mentioned in this invention... PSI It is more sensitive to stress and is more effective in reflecting adverse stress. It is expected to be applied to the early diagnosis of stress in plants, so as to intervene in time and reduce losses.

The invention discloses a rice seedling blast disease intelligent analysis system based on remote sensing data analysis, and relates to the technical field of agricultural remote sensing and plant disease monitoring, the system comprises a physiological data acquisition module, a physiological feature extraction module and a disease severity calculation and severity grading module; wherein the physiological data acquisition module realizes in-situ non-invasive acquisition of the spectral reflectivity of rice seedling leaves, the maximum photochemical efficiency of a light system II and the water transport rate of stalks through various sensors such as a hyperspectral sensor and a chlorophyll luminoscope; the physiological feature extraction module fuses multiple parameters to calculate a leaf surface structure health index and a vascular function activity index; the disease grading module obtains an in-vivo disease load index through weighting, and outputs different disease grades in combination with a K-means clustering threshold value; the system solves the problems that traditional rice seedling blast diagnosis is high in subjectivity and hysteresis quality, and early-stage accurate recognition of diseases is achieved.

This invention relates to the field of fluorescence photometric measurement technology in small optical analytical instruments and optical sensors, and particularly to a coaxial ring-shaped multi-wavelength fluorometer or fluorescence sensor. The invention provides a coaxial ring-shaped light source, consisting of a cylindrical light guide tube and a ring-shaped multi-wavelength LED array located at the bottom of the light guide tube. A fluorescence collecting lens, a set of filters, and a photodetector located within the light guide tube constitute the fluorescence detection unit. The emitted light from the designed light source forms a high-brightness, large-volume illumination region in the transparent medium near the transparent window. Simultaneously, the large-area fluorescence collecting lens significantly improves the fluorescence collection efficiency, thereby enhancing the sensitivity of the fluorescence sensor. Compared with existing multi-wavelength fluorescence sensors, the design of this invention features a simple optical path structure, high detection sensitivity, low manufacturing cost, and wide applicability.

Consumables for use with multi-channel liquid dispensing devices and multi-channel test devices, as well as workflows for their use, are described. The consumables of the present disclosure can include multiple containers pre-loaded with reagents, such as known dilutions of reagents, such as standards, proteins, chemicals, or other desired materials. The multi-channel liquid dispensing device can be used to simultaneously withdraw a fixed amount of reagent from all of the containers in the consumable and simultaneously deposit the reagents into rows of a multi-well device (such as a microwell plate) for further downstream reactions and / or tests. The downstream tests can be in a testing machine, such as a spectrophotometer or fluorometer. The consumables of the present disclosure offer advantages, such as time savings, over individually preparing dilutions of reagents and individually measuring and dispensing the reagents / dilutions into individual wells of a multi-well device for downstream reactions / tests. The consumables of the present disclosure can include multiple rows, with each row including a consumable having multiple containers or tubes.

The present invention measures the content ratios of constituent materials in a product in a non-destructive manner.　This content ratio measurement device measures the content ratios of constituent materials constituting a product, the content ratio measurement device comprising: a content ratio estimation unit that estimates the content ratios of constituent materials constituting a target product for which the content ratios are to be measured, by inputting—to an estimation model that has been trained using fluorescent fingerprint data, measured with a fluorometer with respect to a training product for which the true values of the content ratios are known, and the true values of the content ratios of the training product, the fluorescent fingerprint data consisting of fluorescent intensities corresponding to combinations of excitation wavelengths and fluorescent wavelengths—the fluorescent fingerprint data measured with the fluorometer with respect to the target product; and a pass / fail determination unit that determines whether or not the estimated content ratios of the constituent materials constituting the target product satisfy design standards of the target product.

A spatial gradient-based fluorometer having a signal processor or processing module configured to: receive signals containing information about light reflected by fluorophores in a liquid and detected by a linear sensor array having a length and rows and columns of optical elements; and determine the corresponding signals containing information about a fluorophore concentration of the liquid, a fluorophore concentration of the liquid that depends on a spatial gradient of the light reflected and detected along the length of the linear sensor array, based on the received signals.

The instrument for processing microscopic particles may include a combination of modules integrated into a functional system. The system may be composed of synchronized subsystems including pneumatic modules, fluorometers, multi-wavelength lasers, dual view microscopy, high-voltage generators and other subsystems designed to analyze and control microfluidic processes occurring on a chip. The system performs the combined functions of microfluidic particle generation, analysis, and selection, making it possible for the user to create custom integrated workflows for molecular and cell biology processing. Creating the integrated platform requires an innovative and multi-disciplinary approach for module mechanical integration, electronic synchronization and user interface design for controlling diverse microfluidic processes at high speed.

The application discloses a standard light path system for simulating chlorophyll absorption peaks and a spectral calibration method thereof, and the system comprises a light source module, a light path collimation and coupling module, an integrating sphere homogenization module, a monitoring and feedback module, a spectral calibration module and a control and communication module. The application realizes controllable output in the range of 470-680 nm through the standard light path system, and combines the integrating sphere homogenization technology and the spectral instrument traceability calibration, so that a stable, uniform and traceable standard light source is provided for a chlorophyll fluorometer and other plant physiological measurement devices, the comparability and scientific reliability of measurement data are improved, and thus basic support is provided for ecological system carbon sink monitoring and related instrument metrological calibration.

1. The name of the design product: digital fluorine table (ball valve with exhaust). 2. The use of the design product: intelligent refrigeration tool that can measure pressure, hold pressure test, and measure vacuum. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

1. The name of the design product: digital fluorine table (stop valve with exhaust). 2. The use of the design product: intelligent refrigeration tool that can measure pressure, hold pressure test, and measure vacuum. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.