138 results about "Fluorometer" patented technology

A fluorometer for sensing the fluorescence of a sample utilizes an optical energy source for exciting a sample to be tested and an optical energy detector for detecting the emitted energy from the excited sample. Drive electronics are used for positioning the sample with respect to the optical components allowing a plurality of sample regions to be tested. A processor is utilized to control the operation of the test in accordance with test instructions and for processing the emitted energy detected from the sample to determine test results. A ROM chip socket accepts a plurality of ROM chips, wherein each ROM chip stores test data sets for one or more test types to be performed. ROM chips can be swapped to allow the fluorometer to be configured and reconfigured to perform a plurality of different tests. A communications interface facilitates the sharing of test information between the fluorometer and external entities.

This invention concerns a fluorometer preferably combined with a thermal cycler useful in biochemical protocols such as polymerase chain reaction (PCR) and DNA melting curve analysis. The present flourometer features a low heat-generating light source such as a light emitting diode (LED), having a one-to-one correspondence to each of a plurality of sample containers, such as capped PCR tubes in a standard titer tray. The flourometer of the present invention further comprises an optical path between each LED and its correspondingly positioned container, and another optical path between each flourescing sample within the positioned container and an optical signal sensing means. The instrument can be computer controlled.

A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

The apparatus for measuring concentrations of fuel mixtures using depth-resolved laser-induced fluorescence is a fluorometer equipped with a sample container holder that is movable in the path of the beam from the light source. Fluorescent emissions from the sample mixture pass at 90° to the excitation light path through a slit that is narrow enough that the emission intensity is effectively produced by a thin layer of the sample and focused on a monochromator, with successive thin layers receiving nonuniform excitation radiation due to reduction of intensity along the excitation light source path with increasing depth penetration and due to reabsorption of emitted fluorescence from adjacent layers. The method has a first mode in which the emission spectrum is scanned at a fixed depth, and a second mode in which the sample is moved relative to the emission monochromator slit to vary the depth while keeping the emission wavelength fixed.

A method of fluorometrically monitoring dose control and leak detection, as well as boiler water system programs that occur in industrial systems containing boilers that operate between about 1500 psig and 2600 psig, and at pH from about 7.6 to 11.5.

A water quality sensor for detecting the presence of at least one chemical or biological warfare agent includes: a cell; apparatus for introducing water into the cell and discharging water from the cell adapted for analyzing photosynthetic activity of naturally occurring, free-living, indigenous photosynthetic organisms in water; a fluorometer for measuring photosynthetic activity of naturally occurring, free-living, indigenous photosynthetic organisms drawn into the cell; and an electronics package that analyzes raw data from the fluorometer and emits a signal indicating the presence of at least one chemical or biological warfare agent in the water.

A submersible fluorometer for measuring a fluorescence of a photosynthetic material in a liquid, the fluorometer includes a set of light emitting diodes (LEDs) for supplying light to direct towards the liquid and a photodiode for measuring a detected light emitted from the liquid. The light is used for measuring the fluorescence of the photosynthetic material in the liquid. The fluorometer also has a water-tight volume for protecting the photodiode from the liquid.

A fluorometer with at least one light source, a measuring station with a holder for at least one specimen, a measuring head, and an evaluation station with a detector for evaluating emission signals emitted by a specimen. The measuring head is formed by three optical blocks (A, B, C) assembled in a modular manner. A different measurement method is able to be carried out with each optical block (A, B, C). All the optical blocks (A, B, C) operate the same detector.

The instrument is to be adapted to an ophthalmic slit-lamp (2,6) and includes holders for fluorescence excitation and barrier filters (3,5) to the light path and a detector system made of a linear array of photodiodes (8) with respective circuitry for reading and control. The light is focused in a chosen zone of the eye (13) inducing fluorescence by using specific wavelength. The fluorescence is measured by the detector (8) coupled to one of the eyepieces (12) of the slit-lamp (26) or to a beam-splitter (7) placed before the eyepieces (12). There is a system for digitalization of the signal from the detector (8) which is connected to an IBM compatible computer (11) where the data is stored and analyzed.

A method of detecting target cells and pathogens in a test sample concentrates to target cells in solution by filtering or capturing the target cells on a solid support. The target cells are tagged with a fluorescent dye and dispersed in a solution or suspension. The resulting solution or suspension are introduced to a fluorometer to specifically identify and quantitate the target cells. The target cells can be lysed or whole when introduced to the fluorometer.

A system and method for detecting trace explosives is provided that utilizes a lumnescent reagent, such as blue-fluorescent biphenyl compound, to simultaneously detect multiple nitrogen-based explosives on a sample substrate. The test reagent is mixed with a solvent to improve mixing and maximize the dissolution of the reagent and any trace explosives present on the substrate. A thin film of reagent is applied to the substrate either before or after the substrate contacts a sample area to be tested. Heated air is then applied to the substrate to improve the sample reading. A light source is utilized to illuminate the reagent and expose any quenched portions of the substrate that indicate the presence of an explosive. Quenched portions may be detected visually, or utilizing an analyzing apparatus, such as a fluorimeter or camera. A computer may also be utilized to interpret and record test results.

A novel apparatus and method is described for detection of very small quantities (a few hundred molecules) of bioparticles in nanoliter / picoliter quantities of a sample. The apparatus involves a very small and low cost apparatus that contains a fluorometer. The detection process uses the fluorescence of nanoparticles. Dielectrophoresis is used to concentrate, mix and position the target particles with regard to the light sensor such that maximum detection efficiency is achieved. This allows low cost implementation of low cost point of care tests for disease (animal and plant), infection, food-borne bacteria detection, nucleotide sequencing and pathogen detection (bioterrorism) in real world applications.

A reconfigurable microplate reader comprises a plurality of user installable detection modules. Each module comprises a self-contained detector for a microplate reader. Each module reconfigures the microplate reader to implement at least one specific detection scheme for detecting any of luminescence, fluorescence, and absorbance and / or reconfiguring said microplate reader to implement any of a fluorometer and a luminometer. A microplate reader platform has a port for coupling at least one user installable detection module thereto. The platform also comprises a user interface from the platform to the module, a machine interface from the platform to the module, and a platform configuration mechanism for recognizing a specific detection scheme for an installed detection module and for configuring the platform to support the specific detection scheme.

A method and device for collecting airborne particulate samples comprising vacuum air intake tube(s) onto the distal end of each of which is connected a regulating nozzle that is covered by the well reservoir of a capture vessel. Ambient air is directed to impact the interior surfaces of a single or multi-reservoir capture vessel. Each such well reservoir may include additional collector media and can be fitted with a filter screen. A plurality of said intake tubes may also be assembled and automated for programmable sample times and duration through a manifold in order to service a standard well tray which provides for higher collection efficiencies. In situ pathogen detection is possible by inclusion of nucleic acid specific dyes or probes in the media and / or by attachment of an excitation light, radiation detector, or fluorometer device(s) focused on the internal surfaces of a semi-translucent well reservoir.

This invention concerns a fluorometer preferably combined with a thermal cycler useful in biochemical protocols such as polymerase chain reaction (PCR) and DNA melting curve analysis. The present fluorometer features a low heat-generating light source such as a light emitting diode (LED), having a one-to-one correspondence to each of a plurality of sample containers, such as capped PCR tubes in a standard titer tray. The fluorometer of the present invention further comprises an optical path between each LED and its correspondingly positioned container, and another optical path between each fluorescing sample within the positioned container and an optical signal sensing means. The instrument can be computer controlled.

The present invention provides core technologies necessary for a portable, low cost LED-based handheld fluorometer. The fluorometer is based on a heater integrated within the walls of the reaction chamber, and an orthogonal geometry LED based light source to provide optical excitation. Power is supplied through either an internal power supply, and data is collected in real-time through standard serial interfaces of personal computers, which can also be used to provide power, or personal digital assistants. Thermal regulation is automatically maintained using temperature sensor feedback control. Such a handheld system can allow applications requiring temperature sensitive photometric measurements for real time analyte detection to be performed in the field.

Improved water monitoring systems comprise a test unit having an analytical device and a control system in communication with the test unit such that water supply data generated at the test unit can be communicated to the control system. In some embodiments, the analytical device can be a fluorometer that can monitor the fluorescence induction pattern of algae located in the water, which can indicate, when the induction pattern is compared against known baseline induction patterns, the potential presence of toxic chemicals located in the water supply. In other embodiments, the analytical device can comprise, for example, a pH meter, an infrared spectroscopy (IR) device or the like. In some embodiments, the test unit can provide continuous intake, analysis, and can communicate water supply data to the control system via a wireless communication device, fiber optic cable or the like.

A System and method for self-checking a fluorometer for failure or deteriorated performance includes fluorescent reference standards mounted on a support to move with respect to one or more fixed fluorometers. The intensity of the fluorescent emission of the fluorescent reference standard is initially measured with the fluorometer, and, after a prescribed interval of usage of the fluorometer, a test measurement of the intensity of the fluorescent emission of the fluorescent standard is taken with the fluorometer. The test measurement is compared to the initial measurement, and failure or deteriorated performance of the fluorometer is determined based on a deviation of the test measurement from the initial measurement.

Improved water monitoring systems comprise a test unit having an analytical device and a control system in communication with the test unit such that water supply data generated at the test unit can be communicated to the control system. In some embodiments, the analytical device can be a fluorometer that can monitor the fluorescence induction pattern of algae located in the water, which can indicate, when the induction pattern is compared against known baseline induction patterns, the potential presence of toxic chemicals located in the water supply. In other embodiments, the analytical device can comprise, for example, a pH meter, an infrared spectroscopy (IR) device or the like. In some embodiments, the test unit can provide continuous intake, analysis, and can communicate water supply data to the control system via a wireless communication device, fiber optic cable or the like.

The present invention provides core technologies necessary for a portable, low cost, thermal-regulating LED-based handheld fluorometer. The regulated fluorometer is based on a low thermal mass infrared heater, and an orthogonal geometry LED based filter fluorometer. Power is supplied through an external power supply and data is collected in real-time through standard serial interfaces of personal computers or personal digital assistants. Thermal regulation is automatically maintained using temperature sensor feedback control. Optical excitation relies on LED light source(s) and optical detection is through an adjustable integrating photodetector. Such a handheld system can allow applications requiring temperature sensitive photometric measurements for real time analyte detection to be performed in the field.

The invention designs a quantitative measurement method and device for a transient combustion field and belongs to the technical field of combustion diagnosis. The device comprises a clock synchronization and delay triggering module, a tunable laser generation module, an optical fiber beam splitter, a lens group, a photoelectric detector array, a high-speed multi-path data acquisition module, a high-energy pulse laser generation module, an area array photoelectric detector, a control and calculation module and the like. The optical parameters of a laser-induced fluorescence test system are calibrated by utilizing a steady-state combustion field; the calibrated device is used for measuring the same section of the transient combustion field; and low-resolution temperature distribution obtained by laser absorption spectrum tomography is substituted into an established laser-induced fluorescence calculation model, so that high-resolution temperature and the concentration distribution of measured groups and molecules can be obtained. According to the method and device of the invention, laser-induced fluorescence and a laser absorption spectrum are fused, so that the method and device are of great application value for the monitoring and diagnosis of a combustion field.

Provided are turbidometers and fluorometers having a unique form-factor to accommodate a number of optical components in a confined geometry. This provides the ability to compensate for change in light intensity from an optical source even in a closed-loop manner. The ability to package reference and signal detectors, along with a relatively large diameter LED light source in a confined geometry is particularly suited for applications requiring small-diameter sensors, such as multi-parameter sonde devices having a total diameter that is in the sub-two inch range.

A novel apparatus and method is described for detection of very small quantities (a few hundred molecules) of bioparticles in nanoliter / picoliter quantities of a sample. The apparatus involves a very small and low cost apparatus that contains a fluorometer. The detection process uses the fluorescence of nanoparticles. Dielectrophoresis is used to concentrate, mix and position the target particles with regard to the light sensor such that maximum detection efficiency is achieved. This allows low cost implementation of low cost point of care tests for disease (animal and plant), infection, food-borne bacteria detection, nucleotide sequencing and pathogen detection (bioterrorism) in real world applications.

The present invention relates generally to the field of biochemical laboratory. More particularly the invention relates to the improved and more accurate instrumental features of equipment used as e.g. fluorometers, photometers and luminometers. The object of the invention is achieved by providing an optical measurement instrument where there is an interface (218, 223, 233a, 233b, 238) for a changeable optical module (240), and excitation and / or emission beam is guided through an aperture of the optical module. This allows performing various types of measurements by changing an optical module without a problem lenses becoming unclean due to manual handling of the optical modules. The invention also makes possible to achieve an accurate measurement location and area within a sample.

A fluorescence validation microplate for testing the validity of a fluorometer is provided which can efficiently and cost-effectively test fluorometers to determine linearity, alignment, reproducibility, filter integrity, and cross-talk, each of which is important to proper functioning of the fluorometer.

A detection method includes encapsulating microhole with OTA-BSA, adding OTA standard or sample and OTA antibody, washing out unconnected OTA antibody and EU3+ yangkangtu antiody, adding intensifier to determine fluorescent intensity CPS by time resolution fluorometer, presenting inverse ratio of fluorescent intensity to OTA concentration, comparing to standard curve for obtaining OTA concentration in sample. The kit applying TRFIA to detect OTA based on labelling immune reaction is also disclosed.

A low-cost practical method of determining beryllium or a beryllium compound thereof in a sample is disclosed by measuring fluorescence. This method discloses optical filters for use with low cost fluorometers which may be used to quantitatively determine the presence of beryllium. This method may be extended to estimate particle size distribution for particles comprising Beryllium. A method is also disclosed to store solutions for extended period of time so that these materials are stable during transportation and in the labs.

The apparatus for measuring concentrations of fuel mixtures using depth-resolved laser-induced fluorescence is a fluorometer equipped with a sample container holder that is movable in the path of the beam from the light source. Fluorescent emissions from the sample mixture pass at 90° to the excitation light path through a slit that is narrow enough that the emission intensity is effectively produced by a thin layer of the sample and focused on a monochromator, with successive thin layers receiving nonuniform excitation radiation due to reduction of intensity along the excitation light source path with increasing depth penetration and due to reabsorption of emitted fluorescence from adjacent layers. The method has a first mode in which the emission spectrum is scanned at a fixed depth, and a second mode in which the sample is moved relative to the emission monochromator slit to vary the depth while keeping the emission wavelength fixed.

A method of monitoring a coating applied to a metal surface is disclosed. Specifically, the method comprises the following: applying a sol composition to a metal surface, wherein said composition contains one or more alkoxysilyl group containing compounds, a fluorophore, and a solvent; forming a gelled coating on said surface from said composition; measuring the fluorescence of said coating with a fluorometer, wherein said fluorometer is capable of measuring reflective fluorescence emission measurements; correlating the fluorescence of said coating with the thickness or weight of said coating, and / or with the concentration of alkoxysilyl group containing compound in the coating composition; and optionally applying an additional coating to said metal surface when the thickness of the coating is less than a desired amount or adjusting the concentration of the alkoxysilyl group containing compound applied to said surface.