35 results about "Thermal cycler" patented technology

The invention relates to the technical field of biological detection, in particular to an LAMP (loop-mediated isothermal amplification) detection kit for clostridium difficile tcdA and tcdB. The kit comprises a specific LAMP primer group, an LAMP amplification reaction mixed solution, a lysis solution, a positive reference substance, a negative reference substance and an operation instruction. The system is based on a loop-mediated isothermal amplification (LAMP) principle, nucleic acid detection of toxin-producing genes tcdA and tcdB of clostridium difficile is realized under the condition of 61-65 DEG C, and a detection result is judged through colorimetric change of HNB or a fluorescence signal; according to the system, an amplification reaction can be completed without a thermal cycler, the reaction time is about 50 minutes, and rapid detection can be realized on site; the method disclosed by the invention has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation and low equipment dependence, and is suitable for detecting the toxin-producing gene of the clostridium difficile in medical institutions, primary laboratories and public health monitoring.

The invention relates to the field of agricultural pest drug resistance detection, and provides a detection primer and method for chilo suppressalis ryanodine receptor I4758M mutation and application. According to the detection primer, a mutation recognition site is designed at the 3'tail end of an inner primer BIP, specific detection is achieved through the key base difference (A / G) of a wild type and a mutant type, and the primer group comprises an outer primer pair F3 / B3, an inner primer pair FIP / BIP and a loop primer pair LoopF / LoopB; the detection method is based on loop-mediated isothermal amplification (LAMP), the genotype is visually interpreted through color change of reaction liquid, a thermal cycler is not needed, and the detection method has the advantages of being high in accuracy and sensitivity, easy to operate, low in cost and the like. The matched kit comprises a rapid DNA extraction assembly and is suitable for field on-site detection. The gene mutation frequency of the specific site of the chilo suppressalis can be rapidly detected, and the technical support is provided for resistance monitoring, early warning and scientific treatment of bisamide insecticides.

This invention relates to a thermal unit for temperature management of a dPCR thermal cycler, and also to a thermal unit for a device for simultaneously thermally cycling multiple biological samples, also known as temperature cycling, such a device itself, and a method for simultaneously thermally cycling multiple biological samples using such a device and thermal unit. The thermal unit has at least one heat block (3) and a cooling structure (2), the heat block (3) comprising at least one thermoelectric transducer (31) and a heat transfer plate (33) attached to the thermoelectric transducer (31) for dissipating heat energy from the thermoelectric transducer (31), and the cooling structure (2) comprising a radiator (21), at least one fan (221), and exhaust pipes (24a, 24b).

The invention provides a method for providing skin analysis, and personalized skincare information based on that analysis, to a consumer at a retail point of sale, the method comprising: (i) at the retail point of sale, receiving a sample of the skin microbiome, which is collected from a skin surface of the consumer in a non-invasive manner, (ii) extracting the DNA from the sample of the skin microbiome and analysing the extracted DNA using a portable qPCR thermocycler which is configured to amplify and detect nucleic acids associated with a panel of microorganisms in the sample, (iii) uploading the analysis data from the thermocycler to a machine learning platform, and (iv) providing, at the retail point of sale, a profile of the consumer's skin microbiome and a personalized skincare routine for the consumer, generated from the analysis data using the machine learning platform.

The invention discloses a multiplex nucleic acid detection kit for swine acute diarrhea pathogens and application of the multiplex nucleic acid detection kit. The kit comprises an RAA (Reverse Amplification) primer pair, a specific crRNA (Ribonucleic Acid), a Cas12a protein, an NEBbuffer r2.1 and an ssDNA (Deoxyribose Nucleic Acid) reporter molecule, wherein the RAA primer pair, the specific crRNA, the Cas12a protein, the NEBbuffer r2.1 and the ssDNA reporter molecule are used for detecting the porcine epidemic diarrhea virus (PEDV), the porcine rotavirus (PoRV) and the porcine sapelovirus (PSV). According to the method, reverse transcription, amplification and Cas12a detection processes are coupled through an integrated system or a continuous reaction mode, the whole process is completed under the condition of constant temperature (37 DEG C), thermal cycle equipment is not needed, and only a constant-temperature heating device is needed. After the reaction is finished, a result can be observed through a portable blue light detector or naked eyes, so that on-site real-time visual interpretation is realized.

Systems and methods for processing samples in a multi-well reaction vessel can include inserting a multi-well reaction vessel into a heating chamber of a thermal cycler, enclosing the multi-well reaction vessel in the heating chamber, and compressing a bottom surface of the multi-well reaction vessel into a compliant thermally conductive insert to increase a thermal contact area between the bottom surface and the compliant thermally conductive insert. The compliant thermally conductive insert can be placed between the multi-well reaction vessel and a heating element of the thermal cycler, where heat flux from the heating element passes through the compliant thermally conductive insert to the reaction vessel. The compliant thermally conductive insert can include an elastically deformable creped graphite sheet that can reversibly deform according to different compression profiles depending on the topography or flexure of the reaction vessel and / or heating element.

The present invention relates to a device (10), system (200) and process for the remote calibration of a thermocycler (50).

The invention belongs to the technical field of molecular biological detection, and particularly relates to an RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) and immunochromatography combined PEDV (Porcine Epidemic Diarrhea Virus) nucleic acid detection kit and application thereof. According to the kit, the high sensitivity and high specificity of the loop-mediated isothermal amplification technology and the rapid and visual interpretation advantages of the immunochromatography test strip are combined, and on-site, rapid and accurate detection of the PEDV is achieved. The core of the invention comprises a set of specific primer group designed for a PEDV N gene conserved region, an optimized RT-LAMP reaction system and an immunochromatography test strip which is specially treated and is used for detecting an LAMP amplification product. The kit does not need an expensive thermal cycler, the whole detection process is completed within 40 min, the sensitivity can reach 100 copies / [mu] L, and the kit is particularly suitable for rapid diagnosis and epidemic prevention and control of sites such as pig farms and grassroots veterinary stations.

A sample plate for a thermal cycler suitable for performing a polymerase chain reaction (PCR) procedure includes a base plate and a number of reaction vessels extending upward from the base plate. The sample plate further includes a vertical wall surrounding an outer perimeter defined by the reaction vessels. The vertical wall can be a continuation vertical wall, an intermittent vertical wall, or a perforated vertical wall. The intermittent vertical wall can include a plurality of wall portions, each of which plurality of wall portions is separated from other wall portions via a plurality of gaps.

The present invention relates to a thermal cycler having a thermal module of which the position is adjustable, the thermal cycler comprising: a thermal module for heating and cooling a reaction container; a first support structure to which the thermal module is mounted; a vertical guide part which guides the first support structure to be moved vertically; and a second support structure which supports the vertical guide part and accommodates the first support structure. Thus, a pressure between the reaction container and a heat lid can be uniformly distributed, thereby improving the lifespan of the heat lid and reducing misalignment between components. In addition, provided is the thermal cycler, in which the first support structure is configured such that the thermal module can be attachable / detachable, and the thermal module is attached / detached through one open side of the first support structure. Thus, in the aspect of maintenance, repair and replacement can be carried out easily, and specifications can be changed stably.

The application discloses a mycoplasma pneumoniae 23S rRNA gene A2063G drug resistance mutation detection method based on a CRISPR-LbCas12a system and belongs to the technical field of molecular diagnosis. The detection method can accurately distinguish single base differences such as A2063 and G2063, effectively avoids mismatch, and ensures the accuracy of results. Relying on a one-step reaction of RPA and CRISPR, the total time consumption is less than 45 minutes, and the waiting time is significantly shortened. The whole reaction is carried out under constant temperature conditions, does not need to rely on complex equipment such as a thermal cycler, is easy to operate, is very suitable for the application of primary medical institutions and instant test scenes. The cost control advantage is outstanding, the reagent cost is about 1 / 50 of second-generation sequencing and 1 / 5 of fluorescent PCR, and the economy is extremely good. In addition, the application technology shows good multiplex detection potential and wide application prospect.

The present invention provides a thermal cycler capable of rapidly and efficiently heating and cooling a reaction liquid. The thermal cycler according to the present invention comprises: a temperature control block where a reaction vessel can be installed, a thermoelectric conversion unit capable of heating and cooling, a temperature sensor that measures the temperature of the temperature control block, an insulating substrate that is in contact at one surface with the thermoelectric conversion unit, and a heat radiating unit that is provided on the other surface of the insulating substrate and serves for discharging the heat of the thermoelectric conversion unit to the outside, wherein the temperature control block is heated and cooled by controlling a current or voltage supplied to the thermoelectric conversion unit on the basis of the temperature of the temperature adjustment block measured by the temperature sensor.

The invention discloses a primer and a probe for real-time RPA (recombinase polymerase amplification) detection of goose parvovirus and a detection method. The primer comprises an upstream primer G-F1 and a downstream primer G-R1, the sequences of the upstream primer G-F1 and the downstream primer G-R1 are as shown in SEQ ID No.1-2, the probe is an Exo probe, and the sequence of the Exo probe is as shown in SEQ ID No.3. The detection limit of the detection method established by using the primer and the probe is as low as 3.0 * 10 < 1 > copy number / mu L, cross reaction with other waterfowl viruses is avoided, the clinical coincidence rate reaches 98.1%, detection is completed within 30 min in the whole process, a thermal cycler is not needed, and the method is suitable for field application. The matched kit comprises a primer pair, a probe and an RPA basic reagent, is simple and convenient to operate and controllable in cost, can be used for rapid diagnosis and epidemiological monitoring of GPV classical strains and variants (MDGPV and SBDSV), and has important application value.

The invention relates to a thermoblock for receiving and regulating the temperature of at least one laboratory specimen vessel in a laboratory temperature regulation device, more particularly in a PCR thermocycler, wherein the thermoblock has been produced by means of an additive manufacturing method using a material containing metal. The invention further relates to a method for producing the thermoblock and a computer-implemented method for the simulation of physical characteristics of the thermoblock to be produced in accordance with the invention.

A reaction vessel comprises a lower chamber with a first volume, and an upper chamber with a second volume greater than the first volume. A thermocycling system for heating the reaction vessel includes a lower heating zone to heat the lower chamber, an upper heating zone to heat the upper chamber, and a lid heater to heat an opening of the upper chamber. A method comprises loading a sample into a lower chamber of a reaction vessel, thermocycling the lower chamber using a lower heating zone of the thermo cycler, combining an additive into the sample to produce a combination filling the lower chamber and at least partially filling an upper chamber of the reaction vessel, and incubating the upper and lower chambers using the lower heating zone and an upper heating zone. The lower and upper chambers can have different wall thicknesses to facilitate heat transfer.

The invention relates to the technical field of molecular detection, and particularly discloses an isothermal amplification detection marker, probe, kit and detection method for identifying robinia pseudoacacia honey, and the method comprises the following steps: (1) extracting genome DNA in a honey sample to be detected; (2) the DNA obtained in the step (1) is used as a template, an isothermal amplification reaction system is used for amplification, and amplified target nucleic acid is a robinia pseudoacacia species specific DNA fragment; and (3) carrying out contact reaction on the amplification product in the step (2) and a lateral flow chromatography test strip, and judging whether the honey sample contains the robinia pseudoacacia component or not. The isothermal amplification technology based on the robinia pseudoacacia specific DNA fragment is combined with lateral flow chromatography detection, so that the accuracy and high sensitivity of a detection result are ensured, related species can be effectively distinguished, and trace target components can be detected; the characteristics of constant temperature and rapidness and the visual result interpretation mode get rid of dependence on a precise thermal cycler, and the operation difficulty, the time cost and the equipment threshold are greatly reduced.

The present invention relates to a composite material for a thermal block of a thermal cycler. The composite material has both low specific heat characteristics and remarkably improved thermal conductivity, and thus, when the temperature of a thermal block, which is manufactured using the composite material, rises and falls, deviation in temperature change rate between a plurality of unit thermal blocks and / or in different areas in a unit thermal block can be remarkably reduced such that a PCR thermal cycler including the thermal block can ensure reliability even in ultrafast PCR.

A thermal cycler may include a thermal unit comprising a thermal block for receiving a sample and a thermal element in thermal contact with the thermal block. The thermal cycler may also include a control unit comprising a control circuit board configured to control thermal cycling of the thermal element. The thermal cycler may further include a cooling unit comprising a duct positioned between the thermal unit and the control unit and configured to provide an air flow passage. The thermal cycler may further include a cooling fan configured to provide an air flow to the duct, wherein the thermal unit, the control unit, and the cooling unit are combined into an integrated module.

This disclosure provides a primer set, kit, and their application for rapid visual detection of the soil antibiotic resistance gene TEM-1, as well as a rapid visual detection method. The primer set can rapidly amplify the target gene at room temperature without relying on thermal cycling instruments. The matching crRNA and probe set are precisely matched with the CRISPR / Cas12a restriction enzyme system, significantly improving the detection specificity and sensitivity, and can effectively identify low-abundance target genes in soil. It effectively solves the pain points of existing technologies, such as complex operation, long detection cycle, and high equipment dependence, and provides practical and efficient technical support for the routine monitoring and risk warning of antibiotic resistance genes in the soil environment.

The invention belongs to the technical field of crop disease detection, identification and prevention and treatment, and particularly relates to a primer pair for detecting magnaporthe oryzae and application of the primer pair, and base sequences of the primer pair are shown as SEQ ID NO.1 and SEQ ID NO.2. The rapid detection method for the magnaporthe oryzae is established on the basis of the MHP1-1 primer pair. According to the method, a magnaporthe oryzae MHP gene is taken as a target gene, the sensitivity reaches 10fg / uL after reaction is carried out for 20 minutes at the constant temperature of 38 DEG C, and the specificity is relatively good. The method does not depend on a complex thermal cycler, an experiment can be carried out only through a water bath kettle or a metal bath, the method is suitable for being used in grassroots units and field detection, a simpler and faster technical method is provided for rapid detection and early warning of magnaporthe oryzae, rice germplasm safety and the like, and the method can also be used for rapid detection of other plant diseases.

According to the present invention, the thermal cycler comprises a thermal module which heats and cools a sample of a reaction container provided in a thermal block to perform thermal cycling, a lid assembly which is mounted a heat lid which heats the upper part of the reaction container and is able to move linearly and to rotate in a vertical direction, and a body assembly which has the thermal module mounted thereon and supports the lid assembly.

The present invention relates to a composite material for a thermal block of a thermal cycler. The composite material has both low specific heat characteristics and remarkably improved thermal conductivity, and thus, when the temperature of a thermal block, which is manufactured using the composite material, rises and falls, deviation in temperature change rate between a plurality of unit thermal blocks and / or in different areas in a unit thermal block can be remarkably reduced such that a PCR thermal cycler including the thermal block can ensure reliability even in ultrafast PCR.

An apparatus for controlled separation of a laboratory appliance from a thermal cycler. The thermal block has a slot along a side edge thereof. The rack includes a set of teeth linearly aligned along an upper edge thereof. A gear is positioned to mesh with the set of teeth such that the gear rotates with lateral movement of the rack. A lift pin is associated with the gear, and the lift pin extends from a location offset from a central axis of the gear and toward the thermal block. The lift pin is disposed proximate the side edge of the thermal block such that, in a first position, the lift pin is positioned to rest in the slot and, in a second position, the lift pin is positioned above the thermal block.

This invention discloses a method and kit for isothermal cascade amplification melting curve analysis to detect RNA. The method includes designing a first primer, a second primer, a circular amplification probe, and a detection probe based on the sequence of the circular amplification probe. Isothermal amplification is performed in an amplification system containing the first primer, the second primer, the circular amplification probe, the detection probe, the sample, a nicking enzyme, RNA-dependent DNA polymerase, ribonuclease H, RNA polymerase, and strand displacement polymerase. This invention ingeniously combines an isothermal detection system, RNA polymerase-mediated transcription amplification technology, and a melting curve analysis system, avoiding the heating delay caused by the large heat capacity of the metal module in traditional thermal cyclers, thus significantly reducing the required time and enabling rapid detection.

Systems including thermal cyclers having dual vapor cells and methods of using the systems are described. The system can be used to perform automated polymerase chain reactions (PCRs). The system may include a thermal cycler that may thermally cycle a sample in a PCR cartridge. The thermal cycler may include a thermal element and a first vapor chamber thermally coupled to the thermal element. The first vapor chamber may be thermally coupled to the PCR cartridge during a thermal cycle of the PCR cartridge. The system may include an imager and a processor communicatively coupled with each of the thermal cycler and the imager. The processor may control operation of each of the thermal cycler and the imager to perform a digital PCR. The processor may thermally cycle the sample in the PCR cartridge with the thermal cycler, and image the sample in the PCR cartridge with the imager.

The present invention relates to a micro-thermal cycler comprising at least one micro-reservoir, at least one temperature control assembly, at least one displacement unit, and at least one displacement drive, the at least one displacement unit comprising the at least one micro-reservoir or the at least one temperature control assembly, and the at least one displacement unit being movable along a first direction from a first position to a second position by the displacement drive, and wherein the at least one temperature control assembly thermally interacts with the at least one micro-reservoir in the first position, and the displacement drive operably couples the at least one displacement unit and another temperature control assembly or another micro-reservoir.

Among other things, the present application provides thermal cycler apparatuses and thermal cycling methods that provides heating and cooling of reactions within a reaction cartridge. In certain embodiments, a thermal cycler assembly comprises: (a) a heat source; and (b) at least one heat-exchanging module, each of which include: a thermal well for receiving a reaction cartridge, said thermal well comprising a housing formed of a bottom panel and a first pair of opposing planar side panels spaced apart from a second pair of opposing planar side panels; means for transferring heat from the heat source to the thermal well; and means for cooling the thermal well. Also provided is a reaction cartridge suitable for use with the thermal cycler assembly and methods of using a thermal cycler assembly for performing reactions (e.g., non-isothermal reactions), such as nucleic acid amplification reactions (e.g., non-isothermal nucleic acid reactions).

A device for the controlled release of laboratory equipment from a thermocycler lid. The device includes a sealing plate configured to be fixed to the thermocycler lid. The sealing plate has a top surface and a bottom surface configured to receive the laboratory equipment seal. A pin is mounted on the sealing plate with a spring at its side edge to provide controlled release of the laboratory equipment from the thermocycler lid. The spring is positioned so that when the thermocycler lid is opened, the spring extends the pin in contact with the laboratory equipment, thereby pushing the pin against the upper edge of the equipment and causing the equipment to release from the sealing plate.

The invention discloses a full-automatic high-flux water bath PCR thermal cycler and a control method thereof.The full-automatic high-flux water bath PCR thermal cycler comprises at least three water bath boxes and a heat dissipation assembly, the upper end of each water bath box is open, each water bath box comprises a water tank, a heating assembly, a temperature detection module and a water injection opening, the heating assembly is arranged in the water tank, and the temperature detection module is arranged in the water tank; the temperature detection module is used for detecting the water temperature in each water bath tank, the heat dissipation assembly is used for dissipating heat and cooling at least one water bath tank, and the water injection port is used for being externally connected with a water source; the loading unit is used for loading the reaction plates in batches; the horizontal driving unit is used for driving the loading unit to horizontally move to the position right above each water bath box; the vertical driving unit is used for driving the loading unit to descend to the interior of each water bath box or driving the loading unit to ascend to leave the interior of each water bath box; and a control unit. According to the invention, the treatment flux of the water bath PCR thermal cycler is improved, and the experiment efficiency of water bath PCR is improved.

The application discloses a HPV virus detection kit, which improves the problems of traditional HPV detection, such as the need of expensive instruments, complicated operation and long detection time, by adopting LAMP technology and visual detection design; the kit contains a freeze-drying tube, a liquid reaction tube and a sample lysis tube, LAMP primer groups in the freeze-drying tube are combined with Bst DNA polymerase to realize constant temperature amplification; when the MOPS buffer microspheres in the liquid reaction tube release components to maintain the reaction environment at pH change, the accuracy and stability of the chromogenic accuracy of neutral red are protected, and the neutral red is used as an indicator to intuitively present the result through color (orange yellow negative, red positive); after the sample is mixed with the lysis solution and heated, the reaction system is added at 60 DEG C constant temperature for 30 min, and naked eye judgment can be carried out, without the need of a thermal cycler and other equipment, so that the HPV screening is effectively promoted in the popularization and application of primary medical institutions.