53 results about "Rapid dna" patented technology

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals.

The invention relates to a simple and quick method for extracting DNA from peanut healthy tissues and diseased tissues, which includes two steps. The preparation of sample materials is to take leaves, stem tissues, shell tissues or peanut cotyledons with a weight of 3-10 mg for the test; the sample materials are added to the preset In a 1.5ml centrifuge tube with alkali lysate or enzymolysis solution, grind it with a grinding rod until there are no visible particles; the preparation of DNA extraction refers to the use of alkali boiling method or enzymolysis method to process the above ground sample material, Obtain sample DNA extract. This law is equally applicable to peanut healthy organizations and peanut diseased organizations. Compared with the existing DNA extraction methods, the present invention consumes less time. The time for a single sample in the alkali boiling method does not exceed 20 minutes, and the time for a single sample in the enzymatic hydrolysis method does not exceed 0.5 h; the amount of samples is small, and each sample only needs 3-10 mg; Nitrogen grinding, low cost, can meet the requirements of peanut molecular marker assisted breeding and transgenic breeding. It has no effect on plant growth or seed germination, and is of great significance for the practical application of peanut molecular breeding technology.