6 results about "Rapid dna" patented technology

The invention relates to the technical field of DNA library establishment and sequencing, and particularly discloses a method for performing full-coverage amplification on a DNA sample, a rapid DNA library establishment method, a sequencing method and application thereof. The amplification method comprises the following steps: carrying out low-temperature denaturation PCR (Polymerase Chain Reaction): contacting a DNA (Deoxyribonucleic Acid) sample with a plurality of groups of pre-amplification primer pairs, carrying out PCR amplification, and overlapping an amplification product of any one group of pre-amplification primer pairs in the plurality of groups of pre-amplification primer pairs and an amplification product of any adjacent group of pre-amplification primer pairs with at least 20 basic groups; the library building method comprises the amplification method. The sequencing method comprises the library building method. According to the sequencing method disclosed by the invention, the sequencing result can be quickly and efficiently obtained with high precision and high sequencing depth.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

The invention relates to the field of agricultural pest drug resistance detection, and provides a detection primer and method for chilo suppressalis ryanodine receptor I4758M mutation and application. According to the detection primer, a mutation recognition site is designed at the 3'tail end of an inner primer BIP, specific detection is achieved through the key base difference (A / G) of a wild type and a mutant type, and the primer group comprises an outer primer pair F3 / B3, an inner primer pair FIP / BIP and a loop primer pair LoopF / LoopB; the detection method is based on loop-mediated isothermal amplification (LAMP), the genotype is visually interpreted through color change of reaction liquid, a thermal cycler is not needed, and the detection method has the advantages of being high in accuracy and sensitivity, easy to operate, low in cost and the like. The matched kit comprises a rapid DNA extraction assembly and is suitable for field on-site detection. The gene mutation frequency of the specific site of the chilo suppressalis can be rapidly detected, and the technical support is provided for resistance monitoring, early warning and scientific treatment of bisamide insecticides.

The application discloses a base recognition method based on semiconductor SERS technology and belongs to the technical field of biomedical detection. The specific method comprises the following steps: step one, synthesis of TiO2 nanoparticles; step two, preparation of a TiO2 / 4-MBA system; and step three, base recognition detection. The TiO2 / MBA system can be used for accurate and rapid DNA base recognition, and the developed TiO2 / MBA system is simple to prepare and has excellent base test performance, and has a high market application prospect. The application not only provides a novel base detection method, but also has high sensitivity and selectivity, can recognize and detect four bases and the non-covalent interaction between the four bases and 4-MBA in two pH environments, and has a simple and rapid operation process. The application not only provides a new method for base analysis, but also provides technical support for early diagnosis of DNA related diseases, genomics research and development of molecular sensors, and has a wide application prospect.

The invention relates to the technical field of borosilicate glass, in particular to a sample DNA (deoxyribonucleic acid) rapid extraction method which comprises the following steps: step S1, sample pretreatment: taking 20-100 mu L of liquid sample or 25-50 mg of solid sample, adding 100-200 mu L of pretreatment liquid with the pH value of 7.5-8.0, and uniformly mixing by vortex for 10-30 seconds; s2, synergistic cracking and adsorption: adding 150-200 [mu] L of a composite cracking solution and 10-20 [mu] g of modified diatomite into the pretreatment solution, incubating at 55-60 DEG C for 3-4 min, and reversing and uniformly mixing twice during incubation; s3, impurity removal: adding 150-200 [mu] L of a washing liquid I, standing at room temperature for 1 min, and pouring to remove a supernatant; then adding 200 [mu] L of a washing liquid II, standing for 30 s, and dumping to remove the supernatant. By optimizing the composite lysate, the modified diatomite and reaction conditions, rapid DNA extraction of which the whole process is less than or equal to 8 minutes without protease K digestion and high-speed centrifugation is realized, the method has wide sample adaptability, high automation compatibility and stable DNA yield and purity, the extraction efficiency is greatly improved, and the operation cost and threshold are reduced.

The present disclosure relates to improved methods for preparing, sequencing and analyzing short DNA fragments using handheld, nanopore-based sequencing technology as well as improved methods for extracting DNA, in particular genomic DNA, for any downstream application.