Gene polymorphism detection kit for predicting adverse reaction and curative effect of fluorouracil as well as detection method and application of gene polymorphism detection kit

A technology of genetic polymorphism and detection kits, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome operation and time-consuming, and achieve simplified sequencing process and the effect of time
CN113755588APending Publication Date: 2021-12-07菲思特(上海)生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
菲思特(上海)生物科技有限公司
Publication Date
2021-12-07

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Abstract

The invention discloses a gene polymorphism detection kit for predicting adverse reaction and curative effect of fluorouracil as well as a detection method and application of the gene polymorphism detection kit. The detection kit is used for detecting gene polymorphism of fluorouracil metabolism related genes TYMS, GSTP1, NQO1 and MTHFR, the kit designs specific amplification primers and sequencing primers for the TYMS, GSTP1, NQO1 and MTHFR. The kit comprises the following components: a sample treating fluid, magnetic beads, an amplification reagent 1, an amplification reagent 2, the TYMS, the GSTP1, the NQO1, an MTHFR sequencing primer and a positive control. According to the gene polymorphism detection kit for predicting the adverse reaction and the curative effect of the fluorouracil as well as the detection method and application of the gene polymorphism detection kit, rapid DNA preparation, constant-temperature PCR amplification and pyrosequencing technologies are combined to detect the gene polymorphism for predicting the adverse reaction and curative effect of the fluorouracil, and suggestions from the gene perspective are provided for clinical personalized medication.
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Description

technical field

[0001] The invention relates to a gene polymorphism detection kit for fluorouracil adverse reaction and curative effect prediction, a detection method and application thereof, and belongs to the field of gene detection. Background technique

[0002] Fluorouracil drugs are one of the tumor chemotherapy drugs widely used clinically, including 5-fluorouracil, capecitabine, tegafur, carmofur, floxuridine and deoxyfluridine, etc. These drugs are converted into 5-FU, the main pathway is: converted into 5-fluoro-2-deoxyuridine nucleotides, inhibiting thymine nucleotide synthase, blocking the conversion of deoxyuridine nucleotides into deoxythymine nucleotides, thereby Inhibits DNA biosynthesis. In addition, it can also penetrate into RNA in the form of fluorouridine triphosphate (pseudo-metabolite), inhibit RNA synthesis by preventing uracil and orotic acid from penetrating into RNA, and affect protein biosynthesis, thereby inhibiting the proliferation of granulati...

Claims

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