Rapid DNA extraction kit of medicinal material

A technology of kits and medicinal materials, applied in the field of rapid DNA extraction kits from medicinal materials, which can solve the problems of large differences in plant materials and difficulty in finding formulas for alkali lysing agents
CN104630201AInactive Publication Date: 2015-05-20INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
1 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
Publication Date
2015-05-20
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses a rapid DNA extraction kit of a medicinal material, belongs to the field of the molecular biology, and relates to the traditional Chinese medicine and traditional Chinese medicinal material identification field. The kit comprises a solution A, a solution B, a reagent C and a user manual; and the solution A is composed of a solute triton-100 and a solvent water, the concentration of the solute is 1%, and the final concentration of the solute in the solution A 1%. The solution B is composed of 1M of a solute Tris-HCl and the solvent water, the concentration of the Tris-HCl in the solution B is 1M, the pH value of the solution B is 8.0, the concentration of the solute is 0.1M, and the final solution of the solute in the solution B is 0.1M. The reagent C is composed of NaOH and polyvinylpyrrolidone (PVP, M=40), the final concentration of NaOH in the solution A is 0.5M, and the final concentration of the PVP in the solution A is 1%. The kit is a medicinal material genome DNA extraction product developed for rapidly discriminating the medicinal material, and can be used for rapidly extracting plant and animal medicinal material genome DNA. The kit has the advantages of few operation steps, fast extraction speed, low price, no need of a large device, large-scale high-flux extraction and the like, and is very suitable for rapid detection and identification of the medicinal material DNA.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of identification of traditional Chinese medicines and Chinese medicinal materials, in particular to a rapid DNA extraction kit for medicinal materials. Belonging to the field of molecular biology technology Background technique

[0002] For a long time, DNA extraction has been the most time-consuming and cumbersome step in DNA analysis technology experiments, which seriously restricts the use and promotion of molecular identification methods. In order to achieve the purpose of rapid DNA extraction, researchers have successively proposed ROSE method, magnetic bead adsorption method, Tris-EDTA extraction method, etc. for the extraction of plant DNA. Although these methods shorten the time of DNA extraction, they still cannot achieve the purpose of extracting DNA quickly and easily because they still use steps such as heating, high-speed centrifugation, and precipitation. Among them, the DNA alkaline lysis method is c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More