Application of plant leaf grinding device for high-throughput rapid DNA extraction

A plant leaf, high-throughput technology, applied in the biological field, can solve problems such as time-consuming and labor-intensive, and achieve the effect of being beneficial to DNA extraction, saving sample grinding time and manpower requirements

Inactive Publication Date: 2016-02-03
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that in the rapid DNA extraction method or the direct DNA PCR process, the plant samples ne

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  • Application of plant leaf grinding device for high-throughput rapid DNA extraction
  • Application of plant leaf grinding device for high-throughput rapid DNA extraction
  • Application of plant leaf grinding device for high-throughput rapid DNA extraction

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Embodiment Construction

[0022] The milling device for high-throughput rapid extraction of plant leaf DNA consists of four parts: a milling head 1, a connecting rod 2, a base 3 and a handle 4 ( figure 1 , figure 2 ). It is characterized in that the sample grinding head 1, the connecting rod 2, the base 3 and the handle 4 are sequentially connected. A total of 96 grinding heads 1 are connected to the base of the base through 96 connecting rods ( figure 1 , figure 2 ), the arrangement is 8 rows, 12 columns, A-H: horizontal 8 rows of grinding heads and their connecting rods; I-XII: vertical 12 rows of grinding heads and their connecting rods, with 200 μL 96-well PCR plate and 96-well The PCR machine is completely matched ( figure 2 ).

[0023] When in use, first put the sample into a 200 μL 96-well PCR plate, then freeze the PCR plate in liquid nitrogen for 1-2 minutes, then hold the sample grinder to align the grinding head with the hole of the 96-well PCR plate, and press down 1 to 2 times, th...

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Abstract

The invention relates to an application of a plant leaf grinding device for high-throughput rapid DNA extraction, and belongs to the technical field of biology. The grinding device comprises: a pedestal, a handle, and a plurality of grinding heads and connecting rods, wherein the pedestal is cuboid, is made of a thermal insulation material, and is a device for fixing the connecting rods and the handle; the upper part of the pedestal is connected to the handle; the lower part of the pedestal is connected to the connecting rods; and the lower parts of the connecting rods are connected to the grinding heads. The 96 grinding heads are connected to the base of the pedestal through the 96 connecting rods in an arrangement manner of 8 rows and 12 lines, wherein the transverse distance between two adjacent connecting rods is 0.37-0.9 cm and the longitudinal space distance between two adjacent connecting rods is 0.37-0.9 cm to match a 200 muL 96-hole PCR board and a 96-hole PCR device. For the application of the plant leaf grinding device for high-throughput rapid DNA extraction, whole sample crushing on the 96-hole PCR board can be completed in one step; a requirement of rapid DNA extraction and direct PCR experiment is met; and grinding time is greatly saved and labor requirement is lowered.

Description

1. Technical field [0001] The invention relates to the application of a high-throughput and rapid extraction mill for plant leaf DNA, which belongs to the field of biotechnology. 2. Background technology [0002] With the rapid development of genome projects, the genomes of many plants have been continuously sequenced, such as rice, corn, common wheat, watermelon, tomato, potato, etc. With the completion of genome sequencing, scientists have carried out more extensive and in-depth research on the positioning, cloning and function of important genes. Gene mapping requires a large amount of DNA extracted from plants for PCR amplification. The traditional DNA extraction method is to grind the plant sample into powder by means of a mortar or a mill, and then extract it with a DNA extraction reagent (such as SDS, CTAB, and TPS, etc.) or a DNA extraction kit. The advantage of the traditional method is that high-quality, high-concentration DNA can be extracted. But there are als...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12M1/33
Inventor 张所兵张云辉林静宋春凤方先文
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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