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Globefish skin active collagen and method for preparing peptide thereof

A technology of collagen peptide and collagen, applied in the direction of animal/human protein, specific peptide, connective tissue peptide, etc., can solve environmental pollution and other problems, achieve the effect of solving environmental pollution problems, increasing added value, and high antioxidant activity

Active Publication Date: 2008-05-21
DALIAN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention utilizes non-toxic puffer fish skin to provide a method for preparing puffer fish skin active collagen and its active peptide, so as to achieve high utilization rate of puffer fish skin and maintain the structure and physiological function of the prepared collagen , to prepare highly active puffer fish collagen peptides, which not only solves the environmental pollution problem of puffer fish leftovers, but also improves the added value of the product and broadens the utilization field of puffer fish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of Active Collagen

[0034] After crushing the freeze-dried fish skin, weigh 10g and put it into 50ml, 0.5% sodium chloride solution, stir at 0°C for 12h, then centrifuge at 10000r / min, 0°C for 10min, discard the supernatant, and use Non-collagenous proteins are removed, and the pellet is used to extract active collagen. Put the non-collagen-free fish skin into 50ml, 0.05% acetic acid solution, add 100,000U pepsin at a low temperature of 0°C, enzymolysis time 96h, and then centrifuge at 5000r / min 0°C for 30min. The supernatant was passed through a 5000 ultrafiltration membrane, and the upper part of the ultrafiltration was collected and freeze-dried at -20°C. The obtained puffer fish skin collagen has white color, no sour taste, slightly slight fishy smell, and the purity can reach more than 80%. The prepared collagen can be used in medicine, chemical industry, food and other fields. The precipitate obtained by centrifugation was used to prepare collag...

Embodiment 2

[0038] 1. Preparation of Active Collagen

[0039]After crushing the freeze-dried fish skin, weigh 10g and put it into 1000ml 15% sodium chloride solution, stir at 4°C for 12h, then centrifuge at 10000r / min and 4°C for 30min, discard the supernatant to remove Non-collagen, precipitated to extract active collagen. Put the non-collagen-free fish skin into 1000ml of 1% acetic acid solution, add 10000U of pepsin at a low temperature of 10°C, enzymatically hydrolyze for 96h, and then centrifuge at 5000r / min and 5°C for 10min. The supernatant was passed through a 5000 ultrafiltration membrane, and the upper part of the ultrafiltration was collected and freeze-dried at -80°C. The obtained puffer fish skin collagen has white color, no sour taste, slightly slight fishy smell, and the purity can reach more than 80%. The prepared collagen can be used in medicine, chemical industry, food and other fields. The precipitate obtained by centrifugation was used to prepare collagen active pep...

Embodiment 3

[0043] Preparation of Active Collagen

[0044] 1. Preparation of Active Collagen

[0045] After crushing the freeze-dried fish skin, weigh 10g and put it into 500ml, 5% sodium chloride solution, stir at 2°C for 12h, then centrifuge at 10000r / min, 2°C for 20min, discard the supernatant, and use Non-collagenous proteins are removed, and the pellet is used to extract active collagen. Put the non-collagen-free fish skin into 500ml, 0.5% acetic acid solution, add 60,000U of pepsin at a low temperature of 4°C, enzymatically hydrolyze for 48h, and then centrifuge at 5000r / min at 3°C ​​for 20min. The supernatant was passed through a 10000 ultrafiltration membrane, and the upper part of the ultrafiltration was collected and freeze-dried at -45°C. The obtained puffer fish skin collagen has white color, no sour taste, slightly slight fishy smell, and the purity can reach more than 80%. The prepared collagen can be used in medicine, chemical industry, food and other fields. The precip...

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Abstract

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

Description

technical field [0001] The invention relates to a preparation technology of fish-derived active collagen and its peptide, especially the technical field of treating with microorganisms. Background technique [0002] Studies have shown that active collagen has a variety of physiological functions, and it is widely used in medicine, chemical industry, food and other fields. Blood lipids, anti-tumor, etc. Therefore, people pay much attention to the research and development of collagen and its peptides. The utilization of collagen first started from terrestrial animals, but the collagen products of terrestrial animals are difficult to meet people's needs. Therefore, in recent years, people have turned their attention to aquatic animals with richer collagen resources, especially It is freshwater and seawater fish. Qin Yuqing et al. studied the optimal process conditions for squid skin collagen extraction (Research on Traditional Chinese Medicine, 2002, 1: 20-21), and Wang Jing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06
Inventor 朱蓓薇吕永军任俊凤周大勇张瑜董秀萍李冬梅高全义
Owner DALIAN POLYTECHNIC UNIVERSITY
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