77 results about "Non collagenous protein" patented technology

A resorbable extracellular matrix for reconstruction of cartilage tissue includes a purified collagen II derived from natural cartilage tissue from which non-collagen proteins have been removed. The matrix can be utilized as a scaffold implant for meniscal or vertebral cartilage regeneration.

The invention discloses an extraction and preparation method for micromolecule fishskin collagen peptide, which comprises the following technical steps of: taking the fishskin of freshwater fish as raw material; removing non-collagenous protein; carrying out steps of degreasing, deodorizing, smashing and the like; under the acidic condition, carrying out heating hydrolysis processing to collagenous protein; controlling the degradation degree of the collagenous protein with an enzymolysis method; obtaining a target micromolecule collagen peptide with a membrane separation method; recycling protease, and then carrying out nanofiltration desalting; and after activated carbon binding resin is decolored and deodorized, carrying out spray drying to obtain the colorless and tasteless micromolecule fishskin collagen peptide. The extraction and preparation method disclosed by the invention has a simple technology, the protease is recycled by membrane separation, and therefore the cost is saved. The prepared micromolecule collagen peptide is colorless and tasteless and can be widely applied to industries relevant to the health of the human body, such as health care food, biomedicine, and cosmetics.

The invention discloses a high-purity fishy smell and foreign odor-free fish collagen protein peptide and a preparation method thereof. The preparation method comprises the following steps: cleaning fish skins, and then cutting into blocks and mincing; performing stirring treatment with NaCl solution; centrifugally degreasing to remove paraprotein; adding water to regulate the pH value of initial slurry to be 8.0-8.5; performing combined gradient enzymolysis on alkali protease and neutral protease; deactivating enzyme after the enzymolysis is ended; performing adsorption bleaching by activated carbon; and then performing rough filtration, fine filtration, evaporation concentration and spray drying to prepare the high-purity fishy smell and foreign odor-free fish collagen protein peptide. The high-purity fishy smell and foreign odor-free fish collagen protein peptide disclosed by the invention has a simple process and is easy to industrial production; fishy smell, foreign odor and non-collagen proteins are fully removed in production; the low-temperature biological enzymolysis technology is adopted, and other substances are not added, thereby, the product quality is improved; an obtained fish collagen protein peptide powder has the characteristics the content of amino acid hydroxyproline is not less than 9 percent, and the average molecular weight is lower than 1000Dal; no fishy smell or foreign odor cannot generated, and obvious fishy smell or foreign odor also cannot be generated through heating treatment under the acid conditions. The high-purity fishy smell and foreign odor-free fish collagen protein peptide can be widely applied to processing of various foods and cosmetics as a green functional ingredient.

A process for preparing collagen matrix with heterogeneous bone, used as the bone repairing material and the extracellular matrix of bone tissue engineering, includes such steps as treating ox bone or pig bone, defatting, partial decalcifying, removing non-collagen, proteinas digesting, disinfecting and packing. It has better porosity and pore canal structure.

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

A resorbable extracelluar matrix for reconstruction of cartilage tissue includes a purified collagen II derived from natural cartilage tissue from which non-collagen proteins have been removed. The matrix can be utilized as a scaffold implant for meniscal or vertebral cartilage regeneration.

The invention discloses a method for producing a non-fishy smell collagen membrane by fish processing leftovers, such as fishbone, fish skin, fish scale, fish muscle and the like. The technical process comprises the following steps: removing non collagen, degreasing, removing fishy odor and deashing, stirring and the like to the fish processing leftovers, such as the fishbone, the fishskin, the fish scale, the fish muscle and the like; adding plasticizer in the collagen acquired by hot water extraction and centrifugal separation, stirring, defoaming, drying and the like to prepare the collagen membrane. The invention utilizes fish processing leftovers as main raw materials, and develops a new way for the comprehensive utilization and the product development of the fish processing leftovers; the prepared collagen membrane has good mechanical strength and transparency and no fishy odor, can replace parts of plastic packaging films, can be eaten together with the packaged food, and has good effects on reducing the waste emission of aquatic product processing enterprises, reducing the use of the plastic packaging film in food, and protecting the environment.

The invention discloses a method for extracting collagen from fish skin. The method comprises the following steps of: treating raw materials; degreasing the treated raw materials with isopropanol; removing non-collagen protein with sodium chloride; leaching with acetic acid; adding acid protease for enzymolysis; centrifugally concentrating; and drying so as to obtain fish skin collagen. The collagen is extracted by performing acid extraction and enzymolysis simultaneously, so that extraction rate is high and extraction time is shortened. The fish skin is treated repeatedly with the isopropanol and fat and pigments are removed fully, so that product quality is enhanced. The method of the invention has high collagen extraction rate and bright color, and does not have fat residues.

The invention discloses a method of using carp skin for the enzymatic hydrolysis to prepare collagen, comprising the raw material treatment, non-collagen protein removing by sodium chloride, fat removing by petroleum ether extraction, collagen and papain extracting by citric acid, flavor enzyme common hydrolysis, vacuum concentration and spray drying. Then, carp skin collagen peptide which is white and has no fishy smell with the relative molecular weight of 8,000-15,000 is obtained. The enzyme hydrolysis process of the invention adopts the papain and the flavor protease to simultaneously hydrolyze the carp skin collagen, the enzyme hydrolysis time is shortened greatly which only requires 30min. A petroleum ether extraction method is used for removing the fat. Therefore, fats and residues of fat-soluble pigment in finished product of the carp skin collagen peptide are reduced greatly. The quality of the finished product is improved. The method has the advantages of high yield and collagen peptide extraction rate and perfect product purity.

The invention relates to a method for producing no-fishy smell scale collagen peptide by utilizing macroporous absorbent resin, comprising the following steps of: carrying out acid and alkali treatment on scale to remove hydroxylapatite and non-collagen components, washing the scale to neutrality, adding water 8 to 10 times the weight of the scale and 1-3% enzyme, carrying out enzymolysis for 2 to 3h at the temperature of 45 to 60 DEG C, heating to 90 DEG C, keeping the temperature 10min for inactivating enzymes, decoloring and filtering an enzymolysis liquid through active carbon, carrying out adsorption on clarified hydrolyzate through a column, eluting fishy smell matters through an ethanol solution, eluting the collagen peptide through ammonia water, carrying out membrane separation and concentration on eluted collagen peptide hydrolyzate, freezing and drying to obtain the scale collagen peptide, and carrying out ethanol recovery and sponging drying on ethanol eluate to obtain the fishy smell matters. The invention avoids using chemical agents of bleachers, oxidizers, and the like, and utilizes resin for adsorption by matching with membrane technology and the freezing drying technology to obtain the no-fishy smell collagen peptide and ensures the biological activity of products. In addition, the invention also co-produces the fishy smell matters for developing fisheries condiment.

The invention discloses a tooth mineralization liquid and a tooth mineralization method. The tooth mineralization liquid is used for mineralizing collagen and teeth and comprises two separate parts, namely a reagent A with a compound of non-collagenous protein analog and calcium salts, and a reagent B with phosphates. The preparation of the tooth mineralization liquid requires adjusting pH of thereagents A and B to 5-12. The tooth mineralization method via the tooth mineralization liquid includes smearing the reagent A to the tooth surface, allowing to the smeared reagent A to stand, smearingthe reagent B to the tooth surface, and allowing the smeared reagent B to stand, wherein smearing can be carried out many times as required to arrive at tooth mineralization. In addition, the tooth mineralization liquid also allows the collagen to be biomineralized in the same way; mineralization includes taking single-layer recombinant collagen fibers, collagen gel and collagen sponge, soaking or suspending in the reagent A, taking out, suck-drying with filter paper, and soaking or suspending in the reagent B to arrive at biomineralization, wherein the mineralization can also be performed many times.

The invention provides a preparation method of fish-skin collagen for a medical biomaterial. The preparation method provided by the invention comprises steps of raw material treatment, collagen extraction, extract purification and the like. By the adoption of the preparation method of fish-skin collagen for a medical biomaterial, non-collagen components of fish-skin can be fully and effectively removed; high-purity collagen with the content of obtained hydroxyproline being greater than or equals to 9%, the content of non-collagen being less than or equals to 1% and the content of ash being less than or equals to 1% is obtained; and fish resources can be utilized within a larger range. The preparation method has advantages of simple technology, easy operation, short processing time and obvious biocompatibility effect, and is suitable for industrial production.

The invention relates to a method of quickly repairing demineralized dentine. An existing method of repairing demineralized dentine uses two dentine non-collagen analogs, repairing is implemented by the aid of a Portland cement slow-release system, but this method is too complex and takes as long as four months to achieve full mineralization. According to the method of the invention, polyacrylic acid simple and easy to obtain is used as a regulator, calcium ions and phosphorus ions are used as raw materials, and fluidic amorphous calcium phosphate particles 15-20 nm in size are synthesized; glutamic acid is then used as a mineralization accelerator to mineralize the demineralized dentine again within 2 days, the natural hierarchical structure of the demineralized dentine is repaired, and the mechanical properties of the re-mineralized dentine also close to those of natural dentin. Compared with existing re-mineralization techniques, the method has the advantages that on the basis of achieving equivalent repaired hierarchical structure and mechanical properties, an apparatus is simple, time consumption is low, and the problem of re-mineralizing the demineralized dentine is more effectively solved.

The invention provides a bonding-assisting biological material and applications of the bonding-assisting biological material in biomimetic mineralization, for promoting the remineralization of type I collagen, demineralized enamel and dentin. The bonding-assisting biological mineralization material is the synthetized amorphous calcium phosphate particles stabilized by non-collagenous protein analogue. According to the applications of the bonding-assisting biological mineralization material in biomimetic mineralization, the assisting mineralization material is mixed with a self-etching adhesive, thus the bonding-assisting biological mineralizer is prepared, the bonding-assisting biological mineralizer coats the surfaces of single-layer recombinant type I collagen, the demineralized enamel and the dentin, and then the mineralization property of the biological mineralizer for the single-layer recombinant type I collagen, the demineralized enamel and the dentin is studied. The existing biological mineralization mode (solutions and paste are adopted, namely, gargling or local coating is adopted) is changed, the assisting mineralization material is mixed with the adhesive, after curing, the liquid state is converted into the solid state, then the adhesive anti-caries coating is formed, and the adhesive anti-caries coating can be adhered to the part needing mineralization for a long term, so that the acting time with teeth is prolonged, the use is simple and convenient, and the self-repairing of the demineralized teeth is promoted.

Described, in certain aspects, are medical products comprised of uniquely prepared remodelable extracellular matrix (ECM) materials retaining at least a portion of their native bioactivity. Also described are methods for forming and using such products. In one embodiment, an inventive product comprises a layer of remodelable ECM material modified through contact with periodic acid or a salt thereof. In some forms, such a modified ECM material layer includes non-native Schiff's base crosslinks within and / or between certain components of the ECM material (e.g., between two collagen molecules, two non-collagen molecules, and / or a collagen molecule and a non-collagen molecule). Other inventive products are comprised of various gels, foams, pastes, and formed, coherent, porous bodies at least containing ECM materials that have been modified in accordance with the present invention.

The invention relates to a method for extracting collagen from sturgeon skin, belongs to the technical field of food waste recycling. The method is characterized in that lipide is removed with diluted polybasic weak base, and non-collagen is removed by soaking with 3.5% sodium chloride solution for 24h, so that the collagen activity is not influenced; by adopting pancreatin to extract, the extracting is more sufficient, a best extracting effect is obtained, the collagen purity is improved, and the denaturalization and inactivation of the collagen are not caused due to mild extracting conditions; and no toxic chemicals are added, harmful substance residue is not existed, and no pollution is caused to the collagen and environment, thus the treatment technique is environment-friendly and green. Therefore, the method is simple in process, low in equipment investment and economical, and is suitable for industrial production.

The invention provides a preparation method and application of tuna skin high-moisturizing bioactive peptide, and belongs to the technical field of moisturizing cosmetics. The preparation method of the tuna skin high-moisturizing bioactive peptide comprises the steps that tuna skin is preliminarily crushed, and tuna skin pulp is obtained; the tuna skin pulp is subjected to micro crushing, and fine tuna skin pulp is obtained; after the fine tuna skin pulp and water are mixed, solid-liquid separation is carried out, water-soluble matter of non-collagenous protein is removed, and tuna skin precipitate is obtained; the tuna skin precipitate and enzymes are subjected to enzymatic hydrolysis in a water system, and tuna skin enzymatic hydrolysate is obtained; the enzymatic hydrolysate is separated through an ultrafiltration membrane, active polypeptide with the molecular weight smaller than 2 KD is obtained through separation, and the active peptide with the molecular weight smaller than 2 KD is the tuna skin high-moisturizing bioactive peptide. The preparation method of the tuna skin high-moisturizing bioactive peptide is simple, the process is easy to control, the tuna skin high-moisturizing bioactive peptide is suitable for industrial and large-scale production, and environmental friendliness and high safety are achieved. The prepared tuna skin high-moisturizing bioactive peptide is well applied in the field of the moisturizing cosmetics.

The invention relates to a method for repairing demineralized dentin. A conventional method for repairing demineralized dentin is realized through adopting two dentin non-collagenous protein analogues and having the aid of a portland cement sustained-release system, however, the time required to achieve the whole layer mineralization needs to last four months because the conventional method is excessively complicated. The method comprises the following steps: at first, simple, convenient and readily available polyacrylic acid is taken as a conditioning agent, and calcium ions and phosphonium ions are taken as raw materials, so as to synthetize amorphous calcium phosphate particles with the size of 15 to 20 nm and flowability; and then, the demineralized dentin is remineralized within 7 days to recover the natural hierarchical organization of the demineralized dentin, and the mechanical property of the remineralized dentin reaches the same level with that of the natural dentin. Compared with the conventional remineralization technology, on the basis of reaching the same repaired hierarchical organization and the same mechanical property, the device is simple, the consumed time is short, and the fact that the demineralized dentin is remineraized is more effectively achieved.

The invention discloses a method for extracting unmodified natural collagen from bullfrog skin. The method is characterized by comprising the following steps of: treating washed bullfrog skin by using NaOH solution, H2O2 solution and a degreasing agent in the treatment phase to remove non-collagen protein, pigments and fat; leaching acetic acid solution and acetic acid-protease in the extraction phase to obtain acid-soluble collagen and enzyme-soluble collagen; performing salting-out for many times by utilizing a salt in the purification phase; and dialyzing and performing freeze-drying to obtain spongy bullfrog skin collagen. A lauryl sodium sulfate-polyacrylamide gel electrophoretogram indicates that the bullfrog skin collagen contains a gamma chain with the molecular weight of 0.3 million, a beta chain with the molecular weight of 0.2 million and alpha chains with the molecular weight of 0.1 million, wherein the alpha chains are two, and a triple helix structure of the collagen is better reserved. Unless otherwise specified, the operation temperature is below 15 DEG C in the extraction process, the dry weight extraction rate of the obtained product is more than or equal to 80 percent, and the collagen is white in color and high in quality.

The invention discloses mimic polypeptide of non-collagen protein and application of the mimic polypeptide in biomimetic mineralization. An amino acid sequence of the polypeptide in the invention is represented as SEQ ID NO:1. Function and effect of polypeptide mimic soluble non-collagen protein (BSP and DMP1) in the invention are as follows: controlling deposition and growth of mineral in I type collagen, further simulating formation processes of human body natural hard tissues, promoting mineral nucleation and growth, increasing combination capability of the mineral and collagen fiber, raising mineralization degree of the mineral especially hydroxy apatite in the I type collagen, thereby promoting restoration of bone and dentine.

The invention discloses a hernia bioremediation mesh and a preparation method thereof. The hernia bioremediation mesh is made of animal tissues, immune ingredients such as cells and DNA are removed, and the animal tissues are cut into thin strips, twisted into threads, and woven into the bioremediation mesh. Xenogeneic decellularized materials obtain greater intensity through twisting into the threads, so that the xenogeneic decellularized materials are not prone to breaking; since the threads are interwoven with each other after weaving, external force of tensile tearing does not act on one point but on a plurality of interwoven points, so that the mechanical properties are good; and the defects that the xenogeneic decellularized material hernia bioremediation mesh has poor mechanical intensity and needs to be introduced with cross-linking agents or synthetic materials are overcome. The structure of the mesh is prepared by a weaving process, and the size of a mesh aperture can be easily adjusted through weaving parameters, so that requirements of practical application are met. Meanwhile, the hernia bioremediation mesh retains an original three-dimensional structure, non-collagen proteins, growth factors and other components of an extracellular matrix, so that the functional reconstruction and postoperative healing of the tissues are promoted.

The invention discloses a biomimetic mineralized adhesive film prepared from polymer film forming material-loaded ACP and an in-vitro induction remineralization method. One or more of polymer film forming materials such as hydroxypropylmethylcellulose, chitosan and konjac glucomannan are added into an ethanol aqueous solution or pure water or anhydrous ethanol, one or more stable ACPs of polyelectrolytes such as polyacrylic acid and polyaspartic acid are added into the sol after complete swelling for sol slurry formation, one or more of non-collagen analogues such as glutamic acid and citric acid are added into the mixed sol, the mixture is uniformly stirred so that all the bubbles are removed, a glass slide is uniformly coated with a thin layer of the mixture, and the glass slide with the mixture is put into an oven and is dried and cooled so that the film is formed. The polymer film-forming material carries ACP so that the biomimetic mineralized adhesive film is obtained. The biomimetic mineralized adhesive film can be dried and stored for a long time, can induce re-mineralization of recombinant type I collagen, demineralized enamel and dentin, is easy to use, has a low cost and can be attached to any tooth position needing to be mineralized. The method provides a novel technical method for remineralization repair.

Bovine collagen is freed of non-collagen proteins, glycosaminoglycans and lipids by microbial treatment to yield a product, which is undenatured fibre of high tensile strength. The microbial extraction technique ensures degradation of non-collagenous components with the help of the protease combination produced, leaving the collagenous matter intact due to the absence of collagenase in the secreted enzymes. The resulting atelopeptide collagen has largely monomeric triple helical conformation. This process results in the formation of regularly ordered fibres of collagen possessing a rope-like structure. It is soluble in dilute acidic aqueous solutions. The collagen is rendered non-immunogenic by the removal of certain terminal peptide chains. The non-cytotoxic fibres can be fabricated into various physical forms for biomedical applications.

The invention provides a sturgeon cartilage collagen peptide compound with a skin ultraviolet damage repairing function.The effective constituents of the compound includes, by mass, 25-40 parts of sturgeon cartilage collagen peptide, 5-10 parts of tea polyphenol, 5-10 parts of chitosan oligosaccharide, 5-10 parts of vitamin C and 5-10 parts of vitamin E.A preparing method of the compound comprises the steps of conducting non-collagen removal on sturgeon cartilage with sodium hydroxide, conducting EDTA decalcification, obtaining collagen through acetic acid extraction, conducting alkali-protease hydrolyzation, conducting ultrafiltration to obtain sturgeon cartilage collagen peptide, and obtaining the sturgeon cartilage collagen peptide compound through compatibility of sturgeon cartilage collagen peptide and tea polyphenol, chitosan oligosaccharide, vitamin C and vitamin E according to a certain proportion.The compound has the skin ultraviolet damage repairing function.

The invention discloses a collagen peptide with a light aging relieving effect and a preparation method thereof. The preparation method is characterized in that eel skins are cut up and cleaned, and are then sequentially soaked by a NaOH solution and a butanol solution to remove noncollagen protein and fat; then, an acid method and an enzyme method are used for coextraction to obtain collagen peptide; finally, the collagen peptide is digested through in vitro simulation; and collagen peptide powder is obtained. The method of compounding the acid method and the enzyme method is used for extracting collagen peptide in the eel skins; the extraction rate reaches 80 percent or higher; the purity reaches 85 percent or higher; the collagen peptide obtained through final hydrolysis can relieve symptoms such as skin collagen peptide loss caused by light aging; and good effects of beautification, skin protection and senescence delaying are achieved.

The present invention relates to a method for extracting collagen using ultrasonic wave generator. More particularly, the present invention relates to a method for extracting collagen, comprising: a first step of treating fish skin with ultrasonic waves in the presence of an acid solution to obtain collagen extracts; a second step of separating collagen and the ultrasonically treated fish skin from the collagen extracts; and a step of repeating the first and second steps for the ultrasonically treated fish skin to further extract collagen. In addition, a collagen separation apparatus using ultrasonic waves according to the present invention comprises: a sample tank in which fish skin is accommodated; a separation unit which receives fish skin from the sample tank, separates collagen from the fish skin, and supplies the separated collagen back to the sample tank; and a unit for generating ultrasonic waves, which is connected to the separation unit to generate ultrasonic waves in order to separate collagen from the fish skin. The method for extracting collagen according to the present invention may enable collagen to be extracted using an acid solution having a lower concentration than those used in existing methods, may enable collagen to be extracted at a high yield rate, and may enable collagen having a high molecular weight to be extracted while maintaining the basic structure of the collagen, and not in the form of collagen hydrolysate.

The invention discloses a method for extracting undenatured natural collagen from sucking pig skin. The method is characterized in that treatment is carried out by sub-step repeated different combinations in a manner that a degreasing agent is assisted by intermittent ultrasonic waves, an unhairing agent and a non-collagen removing agent are respectively used for unhairing and removing non-collagen, and no hair root exists in the skin after unhairing, wherein shown by determination, the fat content is lower than 0.5% and the ash content is lower than 0.3%; enzyme-soluble collagen is obtained through leaching by acetic acid-protease and is purified by high-speed centrifugation, sodium chloride salting-out and dialysis, and spongy sucking pig skin collagen is obtained after freeze-drying, wherein the collagen extraction ratio exceeds 50% (dry weight), and shown by electrophoretogram, the sucking pig skin collagen contains a beta chain and an alpha chain, of which the molecular weights are 200000 and 100000 respectively, and is free from other hybrid protein bands, so that the collagen reserves three helical structures and has relatively high purity.

The invention discloses a medical biological yarn, a medical biological repair mesh and a preparation method thereof. The medical biological yarn can be woven into the repair mesh by taking tissues ofan animal as a raw material, removing immune components such as cells and DNA and slicing the raw material into strips and twisting the strips to the yarn. A special cell-removal material is twistedto yarns to obtain greater strength, so that the yarns are hard to break. The yarns are interwoven after being woven, a stretching and tearing external force is not applied to one point but to multiple interwoven points, so that the yarn has good mechanical property. The defect that the mechanical strength of the biological repair mesh is poor and a crosslinking agent or a synthetic material is needed is overcome. Moreover, the structure of the mesh is prepared from a weaving process. The size of the pore diameter of the mesh can be adjusted easily by means of weaving parameters to meet the demand of actual applications. In addition, the medical biological yarn keeps original components such as three-dimensional structures, collagenous fibers, non-collagenous fibers and growth factors in an extracellular matrix and promotes functional reconstruction and postoperation reunion of tissues.

The invention discloses an extraction and preparation method for micromolecule fishskin collagen peptide, which comprises the following technical steps of: taking the fishskin of freshwater fish as raw material; removing non-collagenous protein; carrying out steps of degreasing, deodorizing, smashing and the like; under the acidic condition, carrying out heating hydrolysis processing to collagenous protein; controlling the degradation degree of the collagenous protein with an enzymolysis method; obtaining a target micromolecule collagen peptide with a membrane separation method; recycling protease, and then carrying out nanofiltration desalting; and after activated carbon binding resin is decolored and deodorized, carrying out spray drying to obtain the colorless and tasteless micromolecule fishskin collagen peptide. The extraction and preparation method disclosed by the invention has a simple technology, the protease is recycled by membrane separation, and therefore the cost is saved. The prepared micromolecule collagen peptide is colorless and tasteless and can be widely applied to industries relevant to the health of the human body, such as health care food, biomedicine, and cosmetics.