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Method for producing no-fishy smell scale collagen peptide by utilizing macroporous absorbent resin

A fish scale collagen and collagen peptide technology, which is applied in the field of fish scale collagen peptide production, can solve the problems of product odor, low overall quality, and low activity, and achieve the effect of ensuring activity

Inactive Publication Date: 2010-06-16
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] This process uses pickling and liming to improve the purity of the product, but there is no step of removing fishy smell, coupled with the limitations of enzymatic hydrolysis, concentration and drying technology, the resulting product has peculiar smell, low activity and low overall quality

Method used

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  • Method for producing no-fishy smell scale collagen peptide by utilizing macroporous absorbent resin

Examples

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Effect test

Embodiment 1

[0029] Soak 20kg of fish scales in 0.4mol / L HCl, which is 5 times the weight of the relative fish scales, for 20 hours, wash with water until neutral, then soak in 0.1 mol / L NaOH, which is 5 times the weight of the relative fish scales, for 20 hours, and wash in water until neutral; Double water, hydrolyze 200g of papain for 2.5 hours, hydrolysis pH 7.0, hydrolysis temperature 50°C; after enzymolysis, heat to 90°C, keep for 10 minutes to inactivate the enzyme; the enzymolysis solution is decolorized by activated carbon and then filtered; the obtained clarified enzymolysis solution On the macroporous adsorption resin column, the column loading conditions are pH5.6, the flow rate is 3BV / h, and the column volume is 5BV; after the column is loaded, it is eluted with 2BV20% ethanol at an elution rate of 1BV / h, and then washed with 3BV pure water to The eluent has no obvious ethanol smell, collect ethanol eluate and pure water eluate after alcohol, spray dry after dealcoholization, a...

Embodiment 2

[0031] Soak 20kg of fish scales in 0.4mol / L HCl, which is 5 times the weight of the relative fish scales, for 20 hours. Double water, hydrolyze 400g of animal proteolytic enzyme for 2.5h, hydrolysis pH 6.0, hydrolysis temperature 60°C; after enzymolysis is completed, heat to 90°C and keep for 10 minutes to kill the enzyme; the enzymolysis solution is decolorized by activated carbon and then filtered; The solution is put on a macroporous adsorption resin column, the column loading conditions are pH5.8, the flow rate is 4BV / h, and the column volume is 6BV; after the column is completed, it is eluted with 3BV30% ethanol, and the elution rate is 2BV / h, and then purified with 4BV Wash with water until the eluent has no obvious ethanol smell, collect the ethanol eluate and pure water eluate after alcohol, spray dry after dealcoholization, and obtain 5.1g of fishy smell substance; then elute with 3BVpH7.4 ammonia water, the elution speed is 2BV After washing with 4BV pure water until...

Embodiment 3

[0033] Soak 20kg of fish scales in 0.4mol / L HCl, which is 5 times the weight of the relative fish scales, for 20 hours, wash with water until neutral, then soak in 0.1 mol / L NaOH, which is 5 times the weight of the relative fish scales, for 20 hours, and wash in water until neutral; Double the water, hydrolyze with 600g alkaline protease for 2.5h, the hydrolysis pH is 7.5, and the hydrolysis temperature is 55°C. After the enzymolysis is completed, heat to 90°C and keep for 10 minutes to inactivate the enzyme; the enzymolysis solution is decolorized by activated carbon and then filtered; the obtained clarified enzymolysis solution is put on a macroporous adsorption resin column, and the column conditions are pH 6.0, flow rate 5BV / h, and The column volume is 7BV; after loading the column, use 4BV40% ethanol to elute, the elution speed is 3BV / h, and then wash with 5BV pure water until the eluate has no ethanol smell, collect ethanol eluate and pure water eluate after alcohol, Spr...

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Abstract

The invention relates to a method for producing no-fishy smell scale collagen peptide by utilizing macroporous absorbent resin, comprising the following steps of: carrying out acid and alkali treatment on scale to remove hydroxylapatite and non-collagen components, washing the scale to neutrality, adding water 8 to 10 times the weight of the scale and 1-3% enzyme, carrying out enzymolysis for 2 to 3h at the temperature of 45 to 60 DEG C, heating to 90 DEG C, keeping the temperature 10min for inactivating enzymes, decoloring and filtering an enzymolysis liquid through active carbon, carrying out adsorption on clarified hydrolyzate through a column, eluting fishy smell matters through an ethanol solution, eluting the collagen peptide through ammonia water, carrying out membrane separation and concentration on eluted collagen peptide hydrolyzate, freezing and drying to obtain the scale collagen peptide, and carrying out ethanol recovery and sponging drying on ethanol eluate to obtain the fishy smell matters. The invention avoids using chemical agents of bleachers, oxidizers, and the like, and utilizes resin for adsorption by matching with membrane technology and the freezing drying technology to obtain the no-fishy smell collagen peptide and ensures the biological activity of products. In addition, the invention also co-produces the fishy smell matters for developing fisheries condiment.

Description

technical field [0001] The invention relates to a method for producing collagen peptides from fish scales, in particular to a method for producing fish scale collagen peptides without fishy smell by using a macroporous adsorption resin. Background technique [0002] Collagen is a special protein. In space, collagen consists of three independent protein peptide chains that rely on hydrogen bonds between glycines to form an intertwined helical structure. The triple helix is ​​thin and long, and the space between the helices is very small. In the collagen peptide chain, the amino acids are regularly arranged in the order of - glycine - proline - hydroxyproline - triplet. These characteristics determine that collagen has strong stretching ability and mechanical strength. As an important component of connective tissue in the human body, collagen is the basis for ensuring the health of ligaments and tendons. In addition, it can also keep the skin elastic. Therefore, supplementin...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12P1/00C07K1/14
Inventor 万端极何昌义徐国念李猷任涛
Owner 武汉普赛特膜技术循环利用有限公司
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