22 results about "Collagen hydrolysates" patented technology

The present invention pertains to a cyclic(glycine-proline)-enriched peptide composition and the use thereof particularly for ameliorating neurological conditions or fibrosis. The present invention furthermore pertains to a method for obtaining a peptide composition enriched in cyclic(glycine-proline), the method comprising providing a collagen hydrolysate enriched in gly-pro-x tripeptides, and heat-treating the collagen hydrolysate to allow cyclization of at least part of the gly-pro-x tripeptides to cyclic(glycine-proline).

The invention discloses an extraction method of ganoderma lucidum outer vesicles, the ganoderma lucidum outer vesicles and application of the ganoderma lucidum outer vesicles, and relates to the technical field of extraction methods of plant extracellular vesicles. The extraction method comprises the following steps: inoculating a lucid ganoderma activated strain into a liquid fermentation culture medium for liquid fermentation culture to obtain lucid ganoderma fermentation liquor; carrying out solid-liquid separation on the ganoderma lucidum fermentation liquor to obtain a ganoderma lucidum outer vesicle crude extract; the ganoderma lucidum outer vesicle crude extract is subjected to resuspension precipitation and centrifugal treatment, and ganoderma lucidum outer vesicles are obtained; the liquid fermentation culture medium is prepared from the following components: glucose, maltodextrin, glycerol, a yeast extract, a type III collagen hydrolysate, a grape pomace extract, KH2PO4, Mg2SO4. 7H2O, Ca3 (PO4) 2, FeSO4. 7H2O, ZnSO4. 7H2O, vitamin B1, vitamin B2, chitosan and a surfactant. The method has the advantages that the yield of the ganoderma lucidum outer vesicles is high, the purification method is simple, and the production efficiency is high.

(Main claim): A food supplement comprising collagen hydrolysate and a combination of vitamin C, zinc, vitamin E, silicon dioxide and copper, characterized in that the components are in a composition that causes rapid and uniform dispersion in aqueous liquids and improved bioavailability of the collagen hydrolysate.

This invention discloses a skin-friendly, hypoallergenic composite functional fiber, its preparation method, and its applications, relating to the field of functional textile materials technology. This invention utilizes transglutaminase (TG enzyme) to catalyze the formation of transamide covalent complex macromolecules from chitosan and gelatin, collagen, or collagen hydrolysates. These macromolecules are then blended with spinning dosing and introduced in situ into the fiber matrix through a spinning process. This ensures the functional components are uniformly distributed across the fiber cross-section and surface, forming a continuous, stable, and biocompatible skin-friendly protective film on the fiber surface. Through application, structural, and process innovations, this invention solves the problems of poor wash resistance, easy functional loss, and skin irritation in traditional hypoallergenic textiles. The resulting fiber exhibits long-lasting allergen adsorption, hypoallergenic skin-friendliness, antibacterial and soothing properties, and high wash resistance, making it suitable for intimate apparel, baby products, home textiles, and medical protective textiles.

The application discloses poultry-derived characteristic collagen peptides and application thereof in detection of collagen hydrolysate and products thereof, and belongs to the field of detection. The characteristic collagen peptides are Gly-Leu-Val-Gly-Glu-Hyp-Gly-Pro-Ala-Gly-Ala-Lys, Gly-Glu-Gly-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Ala-Arg and Val-Gly-Pro-Ile-Gly-Pro-Ala-Gly-Asn-Arg, and whether poultry-derived components are contained is determined by detecting one or more characteristic collagen peptides. The characteristic collagen peptides are freed by trypsin enzyme cutting on protein components in a sample, and are detected by a liquid chromatograph-mass spectrometer. The method is characteristic and high in sensitivity, and can be used for determination of poultry-derived components in collagen hydrolysate and products thereof.

The present invention relates to a collagen hydrolysate for use as an active substance in the treatment of neurodermatitis.

The present invention pertains to a cyclic(glycine-proline)-enriched peptide composition and the use thereof particularly for ameliorating neurological conditions or fibrosis. The present invention furthermore pertains to a method for obtaining a peptide composition enriched in cyclic(glycine-proline), the method comprising providing a collagen hydrolysate enriched in gly-pro-x tripeptides, and heat-treating the collagen hydrolysate to allow cyclization of at least part of the gly-pro-x tripeptides to cyclic(glycine-proline).

The application discloses a preparation method of a nano-limited enhanced biomass-based room-temperature phosphorescent material, and specifically comprises the following steps: step 1, white skin powder is added into deionized water, and the pH in the system is adjusted by using a sodium hydroxide solution, and the system is magnetically stirred under heating; after being cooled to room temperature, centrifugation is carried out, and a supernatant, namely a collagen hydrolysate solution, is obtained; step 2, under stirring, two-dimensional layered nanosheets are added into the collagen hydrolysate solution obtained from step 1 and stirred to obtain a transparent solution; and step 3, the transparent solution obtained from step 2 is naturally dried, a light yellow solid is obtained, and grinding is carried out to obtain a two-dimensional layered nano-limited induced enhanced biomass-based room-temperature phosphorescent material. The application further discloses the nano-limited enhanced biomass-based room-temperature phosphorescent material and application thereof. The application solves the problem that the existing water-phase RTP material cannot meet diversified application requirements (anti-counterfeiting and information encryption).

The present invention relates to a cryopreservation preparation comprising collagen hydrolysate, preferably comprising dimethyl sulfoxide.The present invention further relates to a method for cryopreserving one or more biological materials, comprising providing one or more biological materials in a cryopreservation preparation comprising collagen hydrolysate and preferably dimethyl sulfoxide.The present invention also relates to the use of a cryopreservation preparation comprising collagen hydrolysate and preferably dimethyl sulfoxide for cryopreserving one or more biological materials selected from the group consisting of eukaryotic cells, prokaryotic cells, cell organelles, extracellular vesicles, organoids, tissues, and organs.The present invention also relates to the use of collagen hydrolysate in a cryopreservation preparation, preferably in combination with dimethyl sulfoxide.

The invention discloses a preparation method of recombinant III-type collagen based on fish maw, and relates to the technical field of biologication.The preparation method comprises the steps that firstly, the fish maw is hydrolyzed through an acidic hydrolysis method, fish maw collagen hydrolysate is obtained, then collagenase is added for an enzymolysis reaction, III-type collagen is obtained through extraction, then neutral salt is added for a salting-out reaction, and the recombinant III-type collagen is obtained. The preparation method comprises the following steps: preparing a III-type collagen peptide solution by using collagen as a raw material, then utilizing an enzymatic recombination technology and a cross-linking reaction of enzyme to obtain a recombinant III-type collagen peptide solution, and finally concentrating, purifying and drying the recombinant III-type collagen peptide solution to obtain recombinant III-type collagen freeze-dried powder. According to the method, a mode of combining temperature-controlled enzymolysis and acidolysis is adopted, the structure of collagen is prevented from being damaged by high temperature through low-temperature extraction and activity retention, so that the biological activity, high purity and high activity of the collagen are effectively retained, and the recombinant III type collagen peptide with high purity and high activity is obtained through membrane filtration, molecular screening and recombination technologies.

The application discloses a method for mining potential antioxidant collagen peptide based on artificial intelligence technology and relates to antioxidant collagen peptide mining technology. Step one is to collect antioxidant collagen peptide sequences; step two is to construct a positive sample polypeptide sequence set and a negative sample polypeptide sequence set; step three is to use a ProtT5 protein language model in a ProtTrans open source package to perform feature embedding on the positive sample polypeptide sequence set and the negative sample polypeptide sequence set to obtain positive and negative sample features; and step four is to train an antioxidant peptide discrimination model according to the positive and negative sample features. The application can improve the performance of the model as a classifier, improve the accuracy and generalization ability of the classification model, learn useful features from a large amount of protein sequence data, show a very high accuracy on a collagen hydrolysate antioxidant peptide data set independent of a training data set source, and directly mine and screen new antioxidant peptides from a short peptide database with unknown functions.

The application provides a preparation method of collagen fermentation peptides, and belongs to the technical field of collagen bioengineering. The preparation method comprises the following steps: firstly, collagen is added into water and stirred uniformly, then 8-12wt% of a nutrition enhancer is added into a mixed system, pH is adjusted to 5.8-6.2, and a fermentation substrate is obtained; then Lactobacillus rhamnosus and Lactobacillus plantarum are inoculated into the fermentation substrate, dissolved oxygen is controlled to be 20-30% in the first 24 hours, and is reduced to 5-10% in the last 48 hours, and the product after fermentation is filtered and dried to obtain collagen fermentation peptides. Through the synergistic fermentation of the compound strains, the product is rich in 300-1500Da active peptides, Pro-Hyp-Gly and gamma-aminobutyric acid, and has the functions of tyrosinase inhibition, antioxidation, whitening and intestinal flora regulation, the clinical melanin reduction rate reaches 22% in 8 weeks, and the shelf life is prolonged to 18 months.

The present invention relates to a cryopreservation formulation comprising collagen hydrolysate and preferably comprising dimethylsulfoxide. The present furthermore relates to a method for cryopreservation of one or more biological materials, the method comprising the step of providing the one or more biological materials in the cryopreservation formulation comprising collagen hydrolysate and preferably dimethylsulfoxide. The present invention also relates to a use of the cryopreservation formulation comprising collagen hydrolysate and preferably dimethylsulfoxide for cryopreservation of one or more biological materials selected from the group consisting of an eukaryotic cell, a prokaryotic cell, a cell organelle, an extracellular vesicle, an organoid, a tissue, and an organ. The present invention also relates to a use of collagen hydrolysate, preferably in combination with dimethylsulfoxide, in preparing a cryopreservation formulation.

The invention belongs to the technical field of collagen preparation, and particularly relates to a method for carrying out enzymolysis on beef collagen and preparing oligopeptide with physiological activity. The invention provides a method for enzymatic hydrolysis of beef collagen and preparation of oligopeptide with physiological activity, which comprises the following steps: carrying out enzymatic hydrolysis on beef collagen twice by using a compound enzyme to obtain collagen peptide; the two times of enzymolysis comprise hydrolysis with alkaline protease and then hydrolysis with trypsin, so as to obtain the collagen peptide. According to the method disclosed by the invention, step-by-step enzymolysis is carried out by using a compound enzyme, and compared with single-enzyme enzymolysis, the content of collagen oligopeptide in a collagen hydrolysate is increased; furthermore, the compound enzyme with optimized conditions is used for enzymolysis, and the molecular weight of the collagen peptide is lower, so that a product with higher collagen oligopeptide content is prepared; the oxidation resistance and the tyrosinase activity inhibition capability of the collagen peptide solution obtained by the invention are remarkably improved.

The present invention relates to a composition comprising gelatin and collagen hydrolysate. The composition of the present invention has enhanced properties of ease of use in various applications, especially in food applications, in a wide range of temperatures and has enhanced setting time properties. The present invention further relates to an improved process for making said composition and to uses of said composition in food applications, in pharmaceutical applications, technical and in pet food applications. The present invention is particularly useful in the preparation of food applications such as confectionery products and various types of desserts.

The present invention relates to a cryopreservation preparation comprising collagen hydrolysate, preferably comprising dimethyl sulfoxide.The present invention further relates to a method for cryopreserving one or more biological materials, comprising providing one or more biological materials in a cryopreservation preparation comprising collagen hydrolysate and preferably dimethyl sulfoxide.The present invention also relates to the use of a cryopreservation preparation comprising collagen hydrolysate and preferably dimethyl sulfoxide for cryopreserving one or more biological materials selected from the group consisting of eukaryotic cells, prokaryotic cells, cell organelles, extracellular vesicles, organoids, tissues, and organs.The present invention also relates to the use of collagen hydrolysate in a cryopreservation preparation, preferably in combination with dimethyl sulfoxide.

The present invention relates to the use of collagen hydrolysate in the prevention and / or treatment of food craving. The collagen hydrolysate may have a therapeutic and / or non-therapeutic effect. The prevention and / or treatment of craving by the collagen hydrolysate may find purpose in losing bodyweight or improving general well-being. The collagen hydrolysate is preferably administered as a daily food supplement formulation.

A turkey collagen hydrolysate composition is prepared from turkey collagen sources, wherein the composition has: a protein concentration of from about 80 wt % to 100 wt %; a protein molecular weight distribution wherein from about 80% to 100% of the protein in the turkey collagen hydrolysate composition has a molecular weight of from about 500 to about 15,000 Daltons; a protein solubility of from about 97% to 100% at a pH selected from the group consisting of pH 7.0, pH 3.4, pH 5, and all of pH 7.0, pH 5, and pH 3.4; and a protein amino acid content of 3 to 25 glycine (g / 100 g sample), 0.5 to 15 hydroxyproline (g / 100 g sample), 1 to 18 proline (g / 100 g sample), 0.02 to 4 taurine (g / 100 g sample), and 0.05 to 3 tryptophan (g / 100 g sample). Methods of preparing turkey collagen hydrolysate compositions are described.

The present invention relates to a composition comprising gelatin and collagen hydrolysate. The composition of the present invention has enhanced properties of ease of use in various applications, especially in food applications, in a wide range of temperatures and has enhanced setting time properties. The present invention further relates to an improved process for making said composition and to uses of said composition in food applications, in pharmaceutical applications, technical and in pet food applications. The present invention is particularly useful in the preparation of food applications such as confectionery products and various types of desserts.

The present invention relates to cyclic (glycine-proline)-rich peptide compositions and their use, in particular, for ameliorating neurological disorders or fibrosis. The invention also relates to a method for obtaining a cyclic (glycine-proline)-rich peptide composition, said method comprising providing a collagen hydrolysate rich in gly-pro-x tripeptide, and heat treating said collagen hydrolysate to allow at least part of the gly-pro-x tripeptide to be cyclized to cyclic (glycine-proline).

The present invention relates to GPCR ligand collagen peptides produced through microbial fermentation, and in particular, to a method of preparing collagen peptides obtained by culturing a microorganism, peptides prepared by the method, and a composition including the peptide as an active ingredient; and a composition for improving intestinal function, relieving inflammation, and / or promoting wound healing, and a method of preparing the composition.

This invention relates to a composition for anti-skin aging and its application, belonging to the field of biomedical technology. The composition contains two core active ingredients: one is a single-domain antibody that targets and neutralizes human interleukin IL-6, the specific amino acid sequence of which is shown in SEQ ID NO:1; the other is a collagen hydrolysate peptide with a molecular weight of 2-5 kDa. This composition precisely inhibits the core inflammatory signal IL-6 driving aging through the single-domain antibody, while simultaneously replenishing the synthetic matrix and promoting repair using the collagen peptide, resulting in a synergistic effect. Experiments have demonstrated that this composition can significantly improve wrinkles and elasticity in photo-aged skin, promote collagen regeneration, and its collagen peptide component can effectively enhance the skin penetration efficiency of the single-domain antibody. This composition is suitable for preparing safe and effective topical anti-skin aging formulations.