42 results about "Collagen hydrolysates" patented technology

The invention relates to method for obtaining collagen from animal sources such as fresh or saltwater fish, chicken, eggs or from parts of these animal sources that can be considered waste, and to a collagen hydrolysate obtained as a result of this method.

Pet food compositions, and particularly, pet food compositions comprising fish collagen hydrolysate are disclosed herein. Also disclosed herein are methods of treating an immune disorder, such as atopic dermatitis in a companion animal in need thereof and methods of alleviating at least one of pruritus, erythema, or alopecia in a companion animal with atopic dermatitis.

The invention relates to the technical field of biological active peptides, in particular to a method for rapidly and effectively screening light protection active peptides from collagen hydrolysate. The invention relates to an application of a photoprotective polypeptide in preparation of a product. The product has at least one of the following purposes: (1) maintaining or increasing the water content of skin; (2) preventing and / or slowing down skin aging; (3) prevention and / or alleviation of skin UVB injury; and (4) promoting healing of skin wounds or wound surfaces. Two key signal factors (NF-kB, AP-1) and a key matrix metalloproteinase (MMP-9) are screened from a mitogen-activated protein kinase (MAPK) and nuclear factor-kB (NF-kB) pathway to be used as docking targets, and a mimic peptide and the three targets act synergistically to make up for the defect that a single target screening peptide is difficult to comprehensively cope with a light damage multivariate pathway, so that the application of the mimic peptide to the light damage multivariate pathway is realized, and the application of the mimic peptide to the light damage multivariate pathway is realized. And the collagen peptide with excellent light protection activity is obtained through screening.

The invention discloses an application of two collagen peptides with hematopoietic activity, a collagen hydrolysate containing the collagen peptides and a compound of the collagen peptides. The collagen peptides with hematopoietic activity, the collagen hydrolysate containing the collagen peptides and the compound of the collagen peptides comprise one or a combination of the following polypeptides: 1) a polypeptide of GSAGPpGATGFpGAAGR (p is hydroxyproline), and 2) a polypeptide of which the amino acid sequence is GAAGLpGPK (p is hydroxyproline). The invention also provides a preparation method and application of the collagen peptide with hematopoietic activity and the collagen hydrolysate containing the collagen peptide, the two collagen peptides with hematopoietic activity and the compound thereof have good hematopoietic activity and an effect of relieving hematopoietic inhibition, are non-toxic, can be easily used as functional factors to be added into various health-care foods, and have good health-care effects. Good market prospects are realized in medicine and food industries.

The invention provides a bovine type I collagen terminal end peptide characteristic polypeptide, the bovine type I collagen terminal end peptide characteristic polypeptide comprises an N terminal end peptide characteristic polypeptide and a C terminal end peptide characteristic polypeptide, the amino acid sequence of the N terminal end peptide characteristic polypeptide is Q * LSYGYDE, and the amino acid sequence of the C terminal end peptide is LSFLPQPPQE. The invention also provides application of the bovine type I collagen terminal peptide marker polypeptide in detection of N-terminal peptide and C-terminal peptide of bovine type I collagen. The invention also provides a preparation method of a collagen enzymolysis product containing the bovine type I collagen terminal peptide characteristic polypeptide. The bovine type I collagen tail end terminal peptide marker polypeptide provided by the invention is a special terminal peptide at the tail end of bovine type I collagen. The terminal peptide marker polypeptide is used as a marker and can be used for quickly identifying the bovine type I collagen terminal peptide and accurately and quantitatively analyzing the bovine type I collagen terminal peptide. The qualitative and quantitative detection methods are simple to operate and high in sensitivity.

The present invention pertains to a cyclic(glycine-proline)-enriched peptide composition and the use thereof particularly for ameliorating neurological conditions or fibrosis. The present invention furthermore pertains to a method for obtaining a peptide composition enriched in cyclic(glycine-proline), the method comprising providing a collagen hydrolysate enriched in gly-pro-x tripeptides, and heat-treating the collagen hydrolysate to allow cyclization of at least part of the gly-pro-x tripeptides to cyclic(glycine-proline).

To provide a collagen hydrolysate for improving endurance performance and / or for stimulating lipid metabolism.SOLUTION: The present invention relates to the use of a collagen hydrolysate for improving endurance performance by increasing mitochondrial activity. Further, the invention relates to the use of a collagen hydrolysate for stimulating lipid metabolism, and in particular for reducing body weight, by increasing mitochondrial activity.SELECTED DRAWING: Figure 1

The invention discloses an extraction method of ganoderma lucidum outer vesicles, the ganoderma lucidum outer vesicles and application of the ganoderma lucidum outer vesicles, and relates to the technical field of extraction methods of plant extracellular vesicles. The extraction method comprises the following steps: inoculating a lucid ganoderma activated strain into a liquid fermentation culture medium for liquid fermentation culture to obtain lucid ganoderma fermentation liquor; carrying out solid-liquid separation on the ganoderma lucidum fermentation liquor to obtain a ganoderma lucidum outer vesicle crude extract; the ganoderma lucidum outer vesicle crude extract is subjected to resuspension precipitation and centrifugal treatment, and ganoderma lucidum outer vesicles are obtained; the liquid fermentation culture medium is prepared from the following components: glucose, maltodextrin, glycerol, a yeast extract, a type III collagen hydrolysate, a grape pomace extract, KH2PO4, Mg2SO4. 7H2O, Ca3 (PO4) 2, FeSO4. 7H2O, ZnSO4. 7H2O, vitamin B1, vitamin B2, chitosan and a surfactant. The method has the advantages that the yield of the ganoderma lucidum outer vesicles is high, the purification method is simple, and the production efficiency is high.

The invention discloses a high-hydrolysis-degree small-molecular-weight deer sinew collagen peptide and a preparation method thereof. The preparation method comprises the following steps: (1) extracting deer sinew collagen; (2) performing ultrahigh-pressure pretreatment on the deer sinew collagen hydrolysate obtained in the step (1) to obtain ultrahigh-pressure deer sinew collagen liquid, and performing enzymolysis by using alkaline protease under the ultrahigh-pressure treatment conditions that the pressure is 100-400 MPa, the treatment time is 10-40 minutes and the enzyme addition amount of the alkaline protease is 1000-1200 U / g; and (3) the deer sinew collagen hydrolysate obtained in the step (2) is subjected to secondary enzymolysis with flavourzyme, the deer sinew peptide with the high hydrolysis degree is obtained, and the enzyme addition amount of the flavourzyme is 800-1000 U / g. According to the preparation method, the deer sinew collagen peptide is prepared by adopting ultrahigh-pressure synergetic double-enzyme enzymolysis, the spatial conformation of the deer sinew collagen is changed through high-pressure pretreatment, the deer sinew collagen peptide is prepared by synergetic double-enzyme enzymolysis, the hydrolysis degree is remarkably improved, and the low-molecular-weight collagen peptide is accurately enriched.

(Main claim): A food supplement comprising collagen hydrolysate and a combination of vitamin C, zinc, vitamin E, silicon dioxide and copper, characterized in that the components are in a composition that causes rapid and uniform dispersion in aqueous liquids and improved bioavailability of the collagen hydrolysate.

The invention relates to the technical field of after-finishing processing of textiles, and discloses a biological finishing liquid and a finishing method for resisting fuzzing and pilling of fabrics. The biological finishing liquid contains collagen hydrolysate, epoxy resin, a softening agent and a solvent. The invention also discloses an anti-pilling finishing method for the fabric, which comprises the following steps: contacting the fabric with the biological finishing liquid, and drying, the biological finishing liquid contains collagen hydrolysate, epoxy resin, a softening agent and a solvent. When the biological finishing liquid is used for finishing the fabric, the anti-pilling property of the fabric can be improved, and meanwhile, the good hand feeling of the fabric is ensured.

The invention provides a novel method for efficiently extracting and preparing collagen by taking bullfrog skin as a raw material. The process comprises the following steps. The preparation method comprises the following steps: 1) removing impurities from bullfrog skin, cleaning, chopping and homogenizing, stirring and soaking the bullfrog skin with an alkaline solution to remove grease and non-collagen in the bullfrog skin, and washing the bullfrog skin with pure water until the bullfrog skin is neutral; the preparation method comprises 1) preparing collagen powder, 2) hydrolyzing with pepsin and an acetic acid solution, and filtering to obtain a collagen hydrolysate, 3) precipitating to remove macromolecules and non-collagen containing pigment in the collagen hydrolysate by an alkali precipitation method, and centrifuging or filtering to obtain a collagen extracting solution, and 4) concentrating the collagen extracting solution by a membrane, and freeze-drying to obtain the high-purity collagen powder.

This invention discloses a skin-friendly, hypoallergenic composite functional fiber, its preparation method, and its applications, relating to the field of functional textile materials technology. This invention utilizes transglutaminase (TG enzyme) to catalyze the formation of transamide covalent complex macromolecules from chitosan and gelatin, collagen, or collagen hydrolysates. These macromolecules are then blended with spinning dosing and introduced in situ into the fiber matrix through a spinning process. This ensures the functional components are uniformly distributed across the fiber cross-section and surface, forming a continuous, stable, and biocompatible skin-friendly protective film on the fiber surface. Through application, structural, and process innovations, this invention solves the problems of poor wash resistance, easy functional loss, and skin irritation in traditional hypoallergenic textiles. The resulting fiber exhibits long-lasting allergen adsorption, hypoallergenic skin-friendliness, antibacterial and soothing properties, and high wash resistance, making it suitable for intimate apparel, baby products, home textiles, and medical protective textiles.

Pet food compositions, and particularly, pet food compositions comprising fish collagen hydrolysate are disclosed herein. Also disclosed herein are methods of treating an immune disorder, such as atopic dermatitis in a companion animal in need thereof and methods of alleviating at least one of pruritus, erythema, or alopecia in a companion animal with atopic dermatitis.

The application discloses poultry-derived characteristic collagen peptides and application thereof in detection of collagen hydrolysate and products thereof, and belongs to the field of detection. The characteristic collagen peptides are Gly-Leu-Val-Gly-Glu-Hyp-Gly-Pro-Ala-Gly-Ala-Lys, Gly-Glu-Gly-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Pro-Ala-Gly-Ala-Arg and Val-Gly-Pro-Ile-Gly-Pro-Ala-Gly-Asn-Arg, and whether poultry-derived components are contained is determined by detecting one or more characteristic collagen peptides. The characteristic collagen peptides are freed by trypsin enzyme cutting on protein components in a sample, and are detected by a liquid chromatograph-mass spectrometer. The method is characteristic and high in sensitivity, and can be used for determination of poultry-derived components in collagen hydrolysate and products thereof.

Pet food compositions, and particularly, pet food compositions comprising fish collagen hydrolysate are disclosed herein. Also disclosed herein are methods of treating an immune disorder, such as atopic dermatitis in a companion animal in need thereof and methods of alleviating at least one of pruritus, erythema, or alopecia in a companion animal with atopic dermatitis.

The present invention relates to a composition comprising chondroitin sulfate and collagen hydrolysate, for use in ameliorating pain, preferably for use in ameliorating joint pain after exercise. The present invention furthermore relates to a method of preparing said composition by hydrolysis of a collagen-containing material comprising different types of collagen. The composition is preferably administered as a food supplement.

The present invention relates to a cryopreserved formulation comprising a collagen hydrolysate and preferably comprising dimethyl sulfoxide. The invention also relates to a method for cryopreservation of one or more biological substances, said method comprising the step of providing one or more biological substances in a cryopreserved formulation comprising a collagen hydrolysate and preferably dimethyl sulfoxide. The invention also relates to the use of a cryopreservation formulation comprising a collagen hydrolysate and preferably dimethyl sulfoxide for cryopreservation of one or more biological substances selected from the group consisting of eukaryotic cells, prokaryotic cells, organelles, extracellular vesicles, organoids, tissues and organs. The invention also relates to the use of the collagen hydrolysate, preferably the collagen hydrolysate combined with dimethyl sulfoxide, in the preparation of a cryopreservation preparation.

The present invention relates to a collagen hydrolysate for use as an active substance in the treatment of neurodermatitis.

The present invention pertains to a cyclic(glycine-proline)-enriched peptide composition and the use thereof particularly for ameliorating neurological conditions or fibrosis. The present invention furthermore pertains to a method for obtaining a peptide composition enriched in cyclic(glycine-proline), the method comprising providing a collagen hydrolysate enriched in gly-pro-x tripeptides, and heat-treating the collagen hydrolysate to allow cyclization of at least part of the gly-pro-x tripeptides to cyclic(glycine-proline).

The application discloses a preparation method of a nano-limited enhanced biomass-based room-temperature phosphorescent material, and specifically comprises the following steps: step 1, white skin powder is added into deionized water, and the pH in the system is adjusted by using a sodium hydroxide solution, and the system is magnetically stirred under heating; after being cooled to room temperature, centrifugation is carried out, and a supernatant, namely a collagen hydrolysate solution, is obtained; step 2, under stirring, two-dimensional layered nanosheets are added into the collagen hydrolysate solution obtained from step 1 and stirred to obtain a transparent solution; and step 3, the transparent solution obtained from step 2 is naturally dried, a light yellow solid is obtained, and grinding is carried out to obtain a two-dimensional layered nano-limited induced enhanced biomass-based room-temperature phosphorescent material. The application further discloses the nano-limited enhanced biomass-based room-temperature phosphorescent material and application thereof. The application solves the problem that the existing water-phase RTP material cannot meet diversified application requirements (anti-counterfeiting and information encryption).

The present invention relates to a method for manufacturing a paper having improved impermeability to grease and oil, comprising the following steps: (a) providing a paper with a first surface and a second surface; (b) applying an aqueous solution to the first surface, wherein the aqueous solution comprises from approximately 10 to approximately 50 weight % of collagen hydrolysate and at least approximately 0.1 weight % of a plasticizer; (c) drying the aqueous solution in order to obtain a coating comprising collagen hydrolysate and plasticizer on the first surface.

The present invention relates to a cryopreservation preparation comprising collagen hydrolysate, preferably comprising dimethyl sulfoxide.The present invention further relates to a method for cryopreserving one or more biological materials, comprising providing one or more biological materials in a cryopreservation preparation comprising collagen hydrolysate and preferably dimethyl sulfoxide.The present invention also relates to the use of a cryopreservation preparation comprising collagen hydrolysate and preferably dimethyl sulfoxide for cryopreserving one or more biological materials selected from the group consisting of eukaryotic cells, prokaryotic cells, cell organelles, extracellular vesicles, organoids, tissues, and organs.The present invention also relates to the use of collagen hydrolysate in a cryopreservation preparation, preferably in combination with dimethyl sulfoxide.

The invention discloses a preparation method of recombinant III-type collagen based on fish maw, and relates to the technical field of biologication.The preparation method comprises the steps that firstly, the fish maw is hydrolyzed through an acidic hydrolysis method, fish maw collagen hydrolysate is obtained, then collagenase is added for an enzymolysis reaction, III-type collagen is obtained through extraction, then neutral salt is added for a salting-out reaction, and the recombinant III-type collagen is obtained. The preparation method comprises the following steps: preparing a III-type collagen peptide solution by using collagen as a raw material, then utilizing an enzymatic recombination technology and a cross-linking reaction of enzyme to obtain a recombinant III-type collagen peptide solution, and finally concentrating, purifying and drying the recombinant III-type collagen peptide solution to obtain recombinant III-type collagen freeze-dried powder. According to the method, a mode of combining temperature-controlled enzymolysis and acidolysis is adopted, the structure of collagen is prevented from being damaged by high temperature through low-temperature extraction and activity retention, so that the biological activity, high purity and high activity of the collagen are effectively retained, and the recombinant III type collagen peptide with high purity and high activity is obtained through membrane filtration, molecular screening and recombination technologies.

The application discloses a method for mining potential antioxidant collagen peptide based on artificial intelligence technology and relates to antioxidant collagen peptide mining technology. Step one is to collect antioxidant collagen peptide sequences; step two is to construct a positive sample polypeptide sequence set and a negative sample polypeptide sequence set; step three is to use a ProtT5 protein language model in a ProtTrans open source package to perform feature embedding on the positive sample polypeptide sequence set and the negative sample polypeptide sequence set to obtain positive and negative sample features; and step four is to train an antioxidant peptide discrimination model according to the positive and negative sample features. The application can improve the performance of the model as a classifier, improve the accuracy and generalization ability of the classification model, learn useful features from a large amount of protein sequence data, show a very high accuracy on a collagen hydrolysate antioxidant peptide data set independent of a training data set source, and directly mine and screen new antioxidant peptides from a short peptide database with unknown functions.

The application provides a preparation method of collagen fermentation peptides, and belongs to the technical field of collagen bioengineering. The preparation method comprises the following steps: firstly, collagen is added into water and stirred uniformly, then 8-12wt% of a nutrition enhancer is added into a mixed system, pH is adjusted to 5.8-6.2, and a fermentation substrate is obtained; then Lactobacillus rhamnosus and Lactobacillus plantarum are inoculated into the fermentation substrate, dissolved oxygen is controlled to be 20-30% in the first 24 hours, and is reduced to 5-10% in the last 48 hours, and the product after fermentation is filtered and dried to obtain collagen fermentation peptides. Through the synergistic fermentation of the compound strains, the product is rich in 300-1500Da active peptides, Pro-Hyp-Gly and gamma-aminobutyric acid, and has the functions of tyrosinase inhibition, antioxidation, whitening and intestinal flora regulation, the clinical melanin reduction rate reaches 22% in 8 weeks, and the shelf life is prolonged to 18 months.

The present invention relates to a cryopreservation formulation comprising collagen hydrolysate and preferably comprising dimethylsulfoxide. The present furthermore relates to a method for cryopreservation of one or more biological materials, the method comprising the step of providing the one or more biological materials in the cryopreservation formulation comprising collagen hydrolysate and preferably dimethylsulfoxide. The present invention also relates to a use of the cryopreservation formulation comprising collagen hydrolysate and preferably dimethylsulfoxide for cryopreservation of one or more biological materials selected from the group consisting of an eukaryotic cell, a prokaryotic cell, a cell organelle, an extracellular vesicle, an organoid, a tissue, and an organ. The present invention also relates to a use of collagen hydrolysate, preferably in combination with dimethylsulfoxide, in preparing a cryopreservation formulation.

The present invention discloses a computer-aided rapid screening method based on peptidomics, a collagen antifreeze peptide and its application, which relates to the fields of food biotechnology and bioinformatics. The amino acid sequence of the collagen antifreeze peptide is shown as any one of SEQ ID NO.1-5. The present invention provides a computer-aided rapid screening method for antifreeze peptides based on peptidomics. By performing peptidomics analysis on the cod collagen hydrolysate containing a large number of polypeptides, and according to its physicochemical properties, amino acid composition of the primary structure and molecular docking results, a multi-faceted scoring of the interaction ability between the cod collagen hydrolysate and ice crystals is carried out, so as to rapidly screen out antifreeze peptides with strong interaction ability with ice crystals. The antifreeze peptide provided by the present invention can form multiple hydrogen bonds with ice crystals, and has extremely strong interaction ability with ice crystals, and can be used to prepare antifreeze agents, and then applied to frozen surimi products.

The present invention discloses a photocurable hydrogel that can bind cytokines and is used for culturing tumor organoids, and its application, and belongs to the technical field of organoid culture. The present invention develops a hydrogel that can fix cells, bind cytokines, and achieve organoid recovery by using norbornene-modified heparin, norbornene-modified gelatin, thiol four-arm polyethylene glycol, dextran, matrix metalloprotein peptidomimetic MMP, and photoinitiator. Organoids can be directly prepared using this hydrogel, and a variety of structural heparins can be used, which reduces costs. Modified heparin can bind factors or matrix proteins and controllably release factors. Modified gelatin provides amino acid energy for collagen hydrolysate. The hydrogel is formed by photocuring in a step-by-step polymerization manner, and has a controllable structure and adjustable biomimetic modulus and adjustable biomimetic stress relaxation. The hydrogel of the present invention can be used for the cultivation of a variety of organoids, overcoming the problems of low repeatability and instability of current technologies, and is suitable for fields such as tumor drug evaluation.

The invention belongs to the technical field of collagen preparation, and particularly relates to a method for carrying out enzymolysis on beef collagen and preparing oligopeptide with physiological activity. The invention provides a method for enzymatic hydrolysis of beef collagen and preparation of oligopeptide with physiological activity, which comprises the following steps: carrying out enzymatic hydrolysis on beef collagen twice by using a compound enzyme to obtain collagen peptide; the two times of enzymolysis comprise hydrolysis with alkaline protease and then hydrolysis with trypsin, so as to obtain the collagen peptide. According to the method disclosed by the invention, step-by-step enzymolysis is carried out by using a compound enzyme, and compared with single-enzyme enzymolysis, the content of collagen oligopeptide in a collagen hydrolysate is increased; furthermore, the compound enzyme with optimized conditions is used for enzymolysis, and the molecular weight of the collagen peptide is lower, so that a product with higher collagen oligopeptide content is prepared; the oxidation resistance and the tyrosinase activity inhibition capability of the collagen peptide solution obtained by the invention are remarkably improved.