162 results about "Saltwater fish" patented technology

The invention relates to a water circulation system and a method for breeding marine fishes inland, and mainly solves the technical problems of complex process, large investment, high operating cost, large limitation of breeding varieties, low survival rate, slow growth and the like in the process of breeding the marine fishes in fresh water or low salinity salt water at present. A technical scheme comprises the following steps of: a, establishing the water circulation system; b, performing domestication on juvenile fishes of the marine fishes in the low salinity salt water in a gradient mode, namely breeding the juvenile fishes which are bred in the seawater in the brackish water temporarily, formulating a domestication strategy of slowing salinity gradually, and performing domesticationon the juvenile fishes of the marine fishes through three gradients, namely the brackish water phase, the low salinity phase and the ultra-low salinity phase; c, adding a physiological salt preparation, namely adding metal ions which play a key role in osmoregulation into the water or a feed; and d, managing the breeding, namely providing a preference temperature, illumination intensity and a daily illumination period. The water circulation system and the method are mainly used for breeding the marine fishes inland.

The invention relates to a selective breeding method for disease-resistant superior strains of paralichthys olivaceus, which comprises group selective breeding and family selective breeding of the paralichthys olivaceus and is characterized by also comprising molecular marker-assisted selective breeding, manual gynogenesis selective breeding or inbreeding selective breeding of a disease-resistant paralichthys olivaceus family. Multiple kinds of biotechnology such as group selective breeding, intraspecific hybridization, family selective breeding, molecular marker-assisted selective breeding, manual gynogenesis selective breeding, inbreeding and the like is comprehensively adopted; a selective breeding method for the disease-resistant superior strains of marine fishes in China is established for the first time by taking the paralichthys olivaceus as a material; the superior strains of the paralichthys olivaceus of which the vibrio anguillarum resistance is obviously improved are bred; and the breeding survival rate is improved by 20 to 47 percent and the growth rate is improved by over 20 percent. The method opens up a new technical approach for the selective breeding of the disease-resistant strains of the fishes, is suitable for popularization and application to all cultured fishes, has great significance and application value for breeding the disease-resistant superior strains of the cultured fishes, and can generate great economic and social benefits.

A composite feed for redfin puffer is prepared from 15 raw materials including anabarilius powder, concentrated soybean protein, soybean dregs, chicken powder, beer yeast, alpha-starch, soybean lecithin, etc. Its advantages are rich nutrients, high biological utilization rate and survival rate, and less pollution to water.

The invention discloses a special sea fish feed for high-density recirculating aquaculture and a preparation method of the special sea fish feed. The special sea fish feed comprises the following components: white fish meal, fish meal made in china, euphausia superba meal, whey powder, casein, methionine, L-lysine, refined fish oil, cod liver oil, vital gluten, corn starch, peanut meal, special microecologics, special algae powder, functional components, decavitamin and compound trace elements. The special sea fish feed is capable of enhancing the immunity of sea fish, improving the growth speed, the survival rate and the feed conversion efficiency, and reducing the occurrence probability of malformation and albinism, is low in bait coefficient, low in feed cost in the breeding process, and suitable for high-density recirculating aquaculture of the sea fish.

A composite feed for the young turbot is prepared from 14 raw materials including anabarilius powder, spirulina powder, concentrated soybean protein, squid powder, beer yeast, soybean lecithin, etc. Its advantages are rich nutrients, high conversion rate, high growth speed, and less pollution to water.

Disclosed is a Chinese herbal composite prescription for controlling sea fish paralembus digitiformis, wherein the medicinal constituents include (by weight ratio) European verbena 40%, Kulian 30%, baikal skullcap root 30%, the preparing process consists of disintegrating, passing through 100 mesh sieve, and mixing homogeneously.

The invention relates to a saltwater fish compound feed taking microalgae flour as a fat source and a processing method thereof, belonging to the technical field of aquaculture feed processing. The saltwater fish compound feed comprises the following raw materials in parts by weight: 20-40 parts of fish flour, 5-12 parts of casein, 0-5 parts of soybean protein concentrate, 3-8 parts of gluten flour, 10-15 parts of alpha-starch, 5-10 parts of microcrystalline cellulose, 0.4-0.8 part of sulfobetaine, 5-24 parts of microalgae flour, 0.4-0.6 part of sodium carboxymethyl cellulose, 0.02-0.06 part of antioxidant, 0.1-0.3 part of choline chloride, 0.8-1.6 parts of composite vitamin and 1.2-2.5 parts of composite mineral. The processing method comprises the following processing steps: 1) performing wall-breaking on the microalgae flour; 2) preparing the raw materials; 3) pulverizing; 4) performing secondary mixing; 5) blending; 6) granulating through an extrusion bulking machine; 7) drying; and 8) cooling and packaging. According to the invention, the formula is scientific; the process is reasonable; the ratio of the microalgae flour in the compound feed is increased; and the feed can satisfy nutrition required for growth of saltwater fish and can completely replace a saltwater fish compound feed taking fish oil as a fat source.

The invention discloses a preparation method of a high elasticity dietary fiber type seawater fish highly-processed product, which comprises the following steps: unfreezing iced fresh seawater fish by running water, taking the fish, rinsing the fish by deionized water for one to three times, and blending to obtain fish paste; sequentially adding the following components in percentage by weight: 2.5% of table salt, 10% of cassava starch, 1-10% of pigskin collagen and 2-5% of microbial fermentation dietary fiber, controlling system moisture to be 75-80%, and uniformly blending to prepare crude fish paste; performing gelatinization for 45-60 minutes at 30-40 DEG C, heating for 15-30 minutes at 90-100 DEG C, taking out the crude fish paste and putting the crude fish paste into ice water for rapid cooling to obtain the fish paste product. The preparation method is easy and feasible and suitable for industrial production; in addition, the quality of the fish paste product can be remarkably improved, the excellent freeze-thaw stability of dietary fiber ensures that the quick-freeze fish paste product has a longer high-quality shelf life, so that the commercial value of low-value seawater fish is improved.

The invention discloses a separation and purification method of an MBTI. The method comprises the following steps: 1, extraction; 2, neutralization; 3, heat treatment; 4, membrane treatment; and 5, adsorptive separation, and desorption. So the high purity MBTI is obtained. The invention also discloses an application of the MBTI, and the application is that the MBTI, which is added to surimi products treating seawater fish or freshwater fish as a main raw material and is uniformly mixed, is used for enhancing the elasticity of the surimi products. The separation and purification method of the invention has the advantages of simple technology and good separation effect, and the MBTI has a great improvement effect on the elasticity enhancement of the surimi products.

The invention discloses a preparation method of microcapsule feed smaller than or equal to 1.8cm for rhubarb fry, which comprises the following steps: (I) the core material of the formula comprises imported fish meal, bean pulp, rapeseed meal, cuttlefish liver paste, flour and mineral substances, and the wall material of the formula comprises spirulina, fish oil, vitamins, gelatin and water; (II) the core material of the formula is subjected to puffing pretreatment and superfine grinding; (III) the wall material of the formula is homogenized through a colloid mill; (IV) microcapsule bait is prepared by a spray drying method; (V) the proportion of the wall material coating to the core material is (1-10):100; and (VI) the ratio of vitamin C polyphosphate to a chitosan solution for secondary coating is (2-10):100. Experiments prove that the main raw material adopted by the invention is easy to digest and absorb since the microcapsule bait prepared by puffing pretreatment and microcapsule granulation has good food attraction; and moreover, the secondary coating technology can keep the stability and suspension property of bait in water more effectively, thus a broad application prospect of the rhubarb fry initial feed in seawater fish feed is developed.

The invention provides a wild domestication system and domestication method for proliferation and release marine fish fries, and belongs to the technical field of marine biological resource conservation. The domestication system comprises a domestication field, a light source, a flow making pump, an artificial fish reef and a camera system. The system provided by the invention is used for domesticating the ingestion and predation escape capability of the release fries, enhancing the capability of the artificially propagated proliferation and release fries to adapt to the marine natural environment, improving the survival rate of the release fries, and further improving the proliferation effect of seawater fish release.

The invention discloses a method for artificially culturing perineries aibuhitensis in a biological filter. The method is mainly characterized in that: a gravel layer, a coarse sand layer, a fine sand layer and a soil layer are sequentially spread from bottom to top in the filter provided with a water inlet pipe and a drain pipe. Before clamworm seedlings are put into the filter, fresh water is injected into the whole filter, the filter is soaked for 1 week, the fresh water is drained after 1 week, and fresh seawater is injected. The perineries aibuhitensis seedlings are artificially scattered, the size of the seedlings is 3 to 10 joints, and the seedling density is 600 to 2,000 pieces per square meter. The quantity of bait is 8 to 12 percent of the overall weight of the perineries aibuhitensis in the filter, and the bait consists of residual bait and feces of artificially cultured seawater fish, wherein the residual bait and feces are filtered and collected by a silk net of 50 to 100 meshes and concentrated and dried. The temperature of the perineries aibuhitensis culture water in the filter is 4 to 40 DEG C; in 1 to 2 weeks after the seedlings are put, water is not drained, and only water is injected; and after 1 to 2 weeks of putting the seedlings, the water is changed twice every day, and the water in a volume which is 1/3 of the volume of the filter water is artificially changed every time. By the method, the perineries aibuhitensis with high survival rate can be cultured artificially in batch.

The present invention relates to fish cultivation, and is especially cultivation process and special apparatus for converting freshwater fish into saltwater fish. The special apparatus consists of fish pond, dirt intercepting box, water pump, protein decomposing tower, air mixer, waste disposing pipe, filtering box, ultraviolet sterilizer, power supply regulator, etc. The cultivation process includes indoor cultivation, water circulation, four stage salting, four stage filtering, ultraviolet sterilizing, and throwing microbe seed for propagating fish feed. The present invention is one green cultivation process and the produced fish is excellent green food.

The invention relates to a preparation method for subunit vaccines of Cryptocaryon irritans and application of the subunit vaccines of the Cryptocaryon irritans. Approval application and industrialization of the whole organism vaccines of the Cryptocaryon irritans encounter a great challenge, mostly because the parasites cannot be cultured in vitro and only breed on fish bodies, enough Cryptocaryon irritans bodies for producing the vaccines are difficult to obtain, most importantly, the normative production process and the quality standard are difficult to make, and thus, people are forced to conduct research in a deeper level to develop genetic engineering vaccines. Candidate genes of surface proteins C1 are screened from a transcriptome database of the Cryptocaryon irritans, and recombination proteins are expressed on tetrahymena, and accordingly the vaccines are prepared for immunizing seawater fishes; and by detecting the immunogenicity and protectiveness of the vaccines, effective protective antigenic genes of the Cryptocaryon irritans are screened.

The invention discloses an anguilla anguilla liver cell line and a construction method and application thereof, and belongs to the technical field of sea fish cell culture. The cell line is preserved in the Chinese typical culture preservation center on December 16, 2014, and the serial number of preservation registration is CCTCC NO: C2014239. The anguilla anguilla liver cell line is fibroblast cells, the continuous passage achieves more than 60 generations, the adherence after cryopreservation resuscitation is good, the growth is stable, the liver cell line can be directly used for research on aquatic virology, anguilla anguilla cell biological characteristics and immune resistance gene function, and the lever cell line has been proved to be sensitive to eels herpes virus. According to the anguilla anguilla liver cell line and the construction method and application thereof, the constructing method is simple and convenient to implement, the repeatability is high, and the cost is moderate. The anguilla anguilla liver cell line and the construction method and application thereof can also be applied to the preparation of other anguilla anguilla cell lines.

The invention provides application of bracteose eucalyptus essential oil and a complex bacterial agent thereof in preparing an insect repellent for marine fishes. The bracteose eucalyptus essential oil complex bacterial agent comprises the bracteose eucalyptus essential oil and streptomyces griseus HL-50 of which the bacterial content is not smaller than 5*10<8> CFU/g, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No.18424, and the primary effective constituent of the bracteose eucalyptus essential oil is 1,8-eucalyptol. The bracteose eucalyptus essential oil complex bacterial agent is easy to prepare and use, and the effects of effectively expelling various parasites which infect the marine fishes and inhibiting bacteria can be achieved simply by uniformly mixing the bracteose eucalyptus essential oil complex bacterial agent in edible feed for the marine fishes according to the use amount of 0.5-1.5 kg per ton of the feed and continuously feeding the fishes with the feed for 4-7 days; moreover, the complex bacterial agent is poisonless, harmless, and suitable for being used by the marine fishes for a long term, reduces the breeding cost, and has a wideapplication prospect.

The invention discloses cheese shrimp paste. The cheese shrimp paste is prepared from the following raw materials in parts by mass: 50-80 parts of shrimp meat, 20-40 parts of fish meat, 5-10 parts ofhigh-melting point cheese, 2-5 parts of skimmed milk powder, 5-10 parts of water, 5-10 parts of frozen egg white, 1-2 parts of cassava flour, 1-2 parts of water chestnut starch, 0.5-1 part of soybeanmeal, 0.5-2 parts of corn starch, 0.5-2 parts of sweet potato starch, 0.5-1.5 parts of table salt, 0.5-1 part of soy sauce, 2-4 parts of sucrose, 1-2 parts of Shaoxing aged wine and 1-1.5 parts of chicken essence. A preparation method comprises the following steps: granulating live shrimps, granulating two kinds of sea fish, granulating the cheese, adding mixed starch and seasoning to the shrimp granules, fish meat granules and cheese granules according to certain parts by mass, performing uniform stirring and mixing, soaking the mixture in an anti-freezing agent for 3-10 seconds and taking out the mixture; and putting the mixture into plates, and performing freezing forming and packaging. The preparation method is simple and the prepared shrimp paste is smooth in taste, rich in nutrientsand easy to preserve.

The invention discloses compound feed for improving muscle quality of fugu rubripes, amd belongs to the field of marine fish compound feed. The formula of the compound feed comprises the following rawmaterials by mass percent: 42 to 48% of white fish meal; 4 to 5% of yeast; 4 to 6% of krill powder; 4 to 5% vital gluten; 3 to 5% of corn gluten meal; 6 to 8% of soybean meal; 13.1 to 14.5% of wheatflour; 5.5 to 6% of fish oil; 1.5% of phospholipid; 1.5% of calcium dihydrogen phosphate; 1% of vitamin mixture; 1% of mineral mixture; 4.8 to 6.4% of functional additive. Compared with fresh trash fish, the special compound feed disclosed by the invention significantly improves the growth, muscle nutritional value and muscle quality of the fugu rubripes under the urban aquaculture conditions, reduces the dependence on the fresh trash fish in the aquaculture production, and reduces the waste of wild fishery resources and pollution of the cultured marine environment.

The invention relates to a breeding method for increasing survival rate of fish larvae. The method specifically comprises the following steps: (S1) putting the fish larvae into a soil pond, then, carrying out feeding for 3 times separately in the morning and afternoon according to the daily feeding amount of 18g to 22g of rotifers per ten-thousands of fish larvae, and carrying out feeding at the interval of 1 hour for 7 continuous days; (S2) from the eighth day, increasing 10% to 15% of Copepoda every day so as to gradually replace the rotifers until the rotifers are completely replaced, and carrying out feeding according to the daily feeding amount of 30g to 50g of the Copepoda per ten-thousands of the fish larvae at the feeding times and time interval in the step (S1); (S3) after the body length of each fish fry is greater than 1.5cm, increasing 10% to 15% of powdery seawater fish feed every 3 days so as to gradually replace the Copepoda until the Copepoda is completely replaced, and carrying out feeding according to the daily feeding amount of 50g to 80g of the powdery seawater fish feed per ten-thousands of the fish fries at the feeding times and time interval in the step (S1); and (S4) after the body length of each fish fry is greater than 2.5cm, increasing 10% to 15% of zero feed every day so as to gradually replace the powdery seawater fish feed until the powdery seawater fish feed is completely replaced, and carrying out feeding according to the daily feeding amount of 80g to 100g of the zero feed per ten-thousands of the fish fries at the feeding times and time interval in the step (S1).

The invention relates to a method for preparing inactivated vaccine of anti-Listonella-anguillarum egg yolk antibody. The method comprises the following steps: inactivating and centrifuging etiology Listonella-anguillarum BH1 so as to obtain inactivated thallus; preparing Astragalus mongholicus leachate; preparing oil phase by white oil, aluminium stearate and span-80; preparing vaccine water phase by the inactivated thallus and the Astragalus mongholicus leachat; finally, mixing the oil phase with the water phase according to the volume ratio of 2:1, emulsifying and milling for 5 minutes so as to obtain the Vibrio parahaemolyticus oil emulsion inactivated vaccine in which Astragalus mongholicus and oil white are used as united adjuvant. By adoption of the vaccine, the anti-Listonella-anguillarum egg yolk antibody can be obtained; by adoption of the anti-Listonella-anguillarum egg yolk antibody, the immunity of marine fishes such as cynoglossus semilaevis to etiology Listonella-anguillarum is improved, so that the survival rate is improved, the yield is improved, and the economic benefit of culture production of marine fishes such as cynoglossus semilaevis is improved. The problem that the Listonella-anguillarum caused diseases frequently occur in the culture production of marine fishes such as cynoglossus semilaevis, is solved.

The invention relates to an in vitro culture method for scatophagus argus kidney cells. The culture method comprises the following steps of: (1) preparing an L-15 basic culture medium, a primary culture medium, a basic fibroblast growth factor (bFGF)-containing primary culture medium and a subculture medium; (2) performing primary culture on the scatophagus argus kidney cells: a, acquiring a sterile kidney, b, digesting and separating the kidney cells, and c, inoculating; and (3) performing subculture on the scatophagus argus kidney cells. According to the invention, the adopted scatophagus argus kidney cell culture method is strong in repeatability and good in effect; and by reference and improvement on the conventional saltwater fish cell culture method, the culture method suitable for scatophagus argus kidney cell culture is groped, a stable kidney cell culture system is established, a scatophagus argus kidney cell line is established for the first time, a cell model is expected to provide for osmotic pressure regulation of scatophagus argus, and deep research of functional genomes of the scatophagus argus is facilitated.

The invention relates to a litopenaeus vannamei culture method based on a microorganism-aquatic plant-marine fish multistage biological system, wherein high-density culture without changing water canbe achieved. According to the method, a microorganism, aquatic plant and fish multistage culture system is built, staged high-density culture of litopenaeus vannamei is implemented, two stages includea high-density culture stage of a young litopenaeus vannamei pond and an amplification culture stage of a large pond in the development period of young litopenaeus vannamei, the survival rate and theyield of the litopenaeus vannamei are increased, and ecological benefits, economic benefits, resource conservation and the like in a culture system are remarkable.

The invention discloses a method for clearing fouling organisms in a saltwater fish aquaculture net cage. Fish fry is firstly thrown in the saltwater fish aquaculture net cage, wherein cantherines modestus is polycultured according to according to the ratio of 3-8% of cultured fish fry, preferably 5%, at the beginning of cultivation; the individual size of polycultured cantherines modestus is slightly greater than that of the cultured fish fry; and the overall length accounts for 1.3-1.5 times of the overall length of the cultured fish fry. Through the polyculture of cantherines modestus according to a certain ratio, Cantherines modestus continuously ingests the fouling organisms on a net body of the saltwater fish aquaculture net cage so as to continuously clear the net body; the fouling organisms on the net body can be completely removed basically; therefore, the frequency of replacement of the net body of the net cage is reduced, the injuries and disturbances of the cultured fish are effectively reduced, and the risk of diseases of the cultured fish is decreased.

The invention relates to the field of aquatic feed processing, and discloses pelteobagrus fulvidraco culturing feed and a preparation method thereof. The feed provided by the invention is prepared from Peru fish meal, bean pulp, flour, soya-bean oil, monocalcium phosphate, zeolite powder, saltwater fish compound premix, feed grade calcium propionate, vitamin C and an antioxidant. The pelteobagrus fulvidraco culturing feed provided by the invention adopts multiple proper components, fish oil commonly used in a traditional feed formula is removed, meanwhile, a cold spraying technology of cooling the feed first and then spraying grease is adopted for preparation, the prepared feed can reduce the feed coefficients and increase the specific growth rate and relative weight gain rate of pelteobagrus fulvidraco.

The invention discloses a cynoglossus semilaevis derived exosome sandwich ELISA (Enzyme-linked Immuno Sorbent Assay) detection method and a kit and belongs to the technical field of fish biology, particularly the field of application of marine fish exosome markers. According to the method disclosed by the invention, an anti-CD63 antibody serves as an capture antibody, an anti-HSP90 antibody servesas a detection antibody (an enzyme-labelled antibody), and a double-antibody sandwich ELISA method for detecting the cynoglossus semilaevis derived exosome is developed. The invention further provides a kit capable of identifying and quantifying, used for identifying marker proteins of exosome in cynoglossus semilaevis seminal fluid. The kit has important reference significances for identifying exosome of samples derived from other body fluid of fishes as well as other marker proteins. The method disclosed by the invention is high in specificity, high in efficiency and simple and convenient to operate, does not need large instruments or equipment and expensive chemical reagents, and has popularization value.