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170 results about "Saltwater fish" patented technology
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Saltwater fish, also called marine fish, are fish that live in ocean water. Saltwater fish can swim and live alone or live in a large group together, called a school of fish. Saltwater fish are very popular among deep sea fishermen and aquariums all over the country. Saltwater fish are very commonly kept in aquariums for entertainment. Many saltwater fish are also caught to be eaten.
Practicalization method for frozen preserving sperm of fish
InactiveCN1596670ASimple compositionConvenient sourceMeat/fish preservation by freezing/coolingSebastesSeawater
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Water circulation system and method for breeding marine fishes inland
ActiveCN101933489AActivates and strengthens osmoregulatory mechanismsImprove adaptabilityClimate change adaptationPisciculture and aquariaJuvenile fishFresh water organism
The invention relates to a water circulation system and a method for breeding marine fishes inland, and mainly solves the technical problems of complex process, large investment, high operating cost, large limitation of breeding varieties, low survival rate, slow growth and the like in the process of breeding the marine fishes in fresh water or low salinity salt water at present. A technical scheme comprises the following steps of: a, establishing the water circulation system; b, performing domestication on juvenile fishes of the marine fishes in the low salinity salt water in a gradient mode, namely breeding the juvenile fishes which are bred in the seawater in the brackish water temporarily, formulating a domestication strategy of slowing salinity gradually, and performing domesticationon the juvenile fishes of the marine fishes through three gradients, namely the brackish water phase, the low salinity phase and the ultra-low salinity phase; c, adding a physiological salt preparation, namely adding metal ions which play a key role in osmoregulation into the water or a feed; and d, managing the breeding, namely providing a preference temperature, illumination intensity and a daily illumination period. The water circulation system and the method are mainly used for breeding the marine fishes inland.
Owner:SUZHOU FISHSEEDS BIOLOGICAL TECH CO LTD
Selective breeding method for disease-resistant superior strains of paralichthys olivaceus
ActiveCN101911918AGood effectIncreased disease powerClimate change adaptationPisciculture and aquariaSocial benefitsMarine fish
The invention relates to a selective breeding method for disease-resistant superior strains of paralichthys olivaceus, which comprises group selective breeding and family selective breeding of the paralichthys olivaceus and is characterized by also comprising molecular marker-assisted selective breeding, manual gynogenesis selective breeding or inbreeding selective breeding of a disease-resistant paralichthys olivaceus family. Multiple kinds of biotechnology such as group selective breeding, intraspecific hybridization, family selective breeding, molecular marker-assisted selective breeding, manual gynogenesis selective breeding, inbreeding and the like is comprehensively adopted; a selective breeding method for the disease-resistant superior strains of marine fishes in China is established for the first time by taking the paralichthys olivaceus as a material; the superior strains of the paralichthys olivaceus of which the vibrio anguillarum resistance is obviously improved are bred; and the breeding survival rate is improved by 20 to 47 percent and the growth rate is improved by over 20 percent. The method opens up a new technical approach for the selective breeding of the disease-resistant strains of the fishes, is suitable for popularization and application to all cultured fishes, has great significance and application value for breeding the disease-resistant superior strains of the cultured fishes, and can generate great economic and social benefits.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Composite fish bait for red-fin Eastern-Globefish
InactiveCN1618318AMeet nutritional needsImprove water resistanceClimate change adaptationAnimal feeding stuffBiotechnologySeawater
A composite feed for redfin puffer is prepared from 15 raw materials including anabarilius powder, concentrated soybean protein, soybean dregs, chicken powder, beer yeast, alpha-starch, soybean lecithin, etc. Its advantages are rich nutrients, high biological utilization rate and survival rate, and less pollution to water.
Owner:OCEAN UNIV OF CHINA
Special sea fish feed for high-density recirculating aquaculture and preparation method of special sea fish feed
InactiveCN104585525ANutritional balancePromote digestion and absorptionAnimal feeding stuffPeanut mealFish oil
The invention discloses a special sea fish feed for high-density recirculating aquaculture and a preparation method of the special sea fish feed. The special sea fish feed comprises the following components: white fish meal, fish meal made in china, euphausia superba meal, whey powder, casein, methionine, L-lysine, refined fish oil, cod liver oil, vital gluten, corn starch, peanut meal, special microecologics, special algae powder, functional components, decavitamin and compound trace elements. The special sea fish feed is capable of enhancing the immunity of sea fish, improving the growth speed, the survival rate and the feed conversion efficiency, and reducing the occurrence probability of malformation and albinism, is low in bait coefficient, low in feed cost in the breeding process, and suitable for high-density recirculating aquaculture of the sea fish.
Owner:QINGDAO GREAT SEVEN BIO TECH CO LTD
Composite fish bait for fry of brill
InactiveCN1618317AGrow fastImprove survival rateClimate change adaptationAnimal feeding stuffFeed conversion ratioSeawater
A composite feed for the young turbot is prepared from 14 raw materials including anabarilius powder, spirulina powder, concentrated soybean protein, squid powder, beer yeast, soybean lecithin, etc. Its advantages are rich nutrients, high conversion rate, high growth speed, and less pollution to water.
Owner:OCEAN UNIV OF CHINA
Chinese herbal composite prescription for controlling sea fish paralembus digitiformis
Disclosed is a Chinese herbal composite prescription for controlling sea fish paralembus digitiformis, wherein the medicinal constituents include (by weight ratio) European verbena 40%, Kulian 30%, baikal skullcap root 30%, the preparing process consists of disintegrating, passing through 100 mesh sieve, and mixing homogeneously.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Composite bait for brill
InactiveCN1618319AGrow fastImprove survival rateClimate change adaptationAnimal feeding stuffNutrientYeast
A composite feed for adult turbot is prepared from 13 raw materials including anabarilius powder, soybean dregs, peanut dregs, squid powder, beer yeast, soybean lecithin etc. Its advantages are rich nutrients, high conversion rate, high growth speed and survival rate, and less pollution to water.
Owner:OCEAN UNIV OF CHINA
Saltwater fish compound feed taking microalgae flour as fat source and processing method thereof
The invention relates to a saltwater fish compound feed taking microalgae flour as a fat source and a processing method thereof, belonging to the technical field of aquaculture feed processing. The saltwater fish compound feed comprises the following raw materials in parts by weight: 20-40 parts of fish flour, 5-12 parts of casein, 0-5 parts of soybean protein concentrate, 3-8 parts of gluten flour, 10-15 parts of alpha-starch, 5-10 parts of microcrystalline cellulose, 0.4-0.8 part of sulfobetaine, 5-24 parts of microalgae flour, 0.4-0.6 part of sodium carboxymethyl cellulose, 0.02-0.06 part of antioxidant, 0.1-0.3 part of choline chloride, 0.8-1.6 parts of composite vitamin and 1.2-2.5 parts of composite mineral. The processing method comprises the following processing steps: 1) performing wall-breaking on the microalgae flour; 2) preparing the raw materials; 3) pulverizing; 4) performing secondary mixing; 5) blending; 6) granulating through an extrusion bulking machine; 7) drying; and 8) cooling and packaging. According to the invention, the formula is scientific; the process is reasonable; the ratio of the microalgae flour in the compound feed is increased; and the feed can satisfy nutrition required for growth of saltwater fish and can completely replace a saltwater fish compound feed taking fish oil as a fat source.
Owner:AQUATIC PROD RES INST SHANDONG PROV
Preparation method of high elasticity dietary fiber type seawater fish highly-processed product
The invention discloses a preparation method of a high elasticity dietary fiber type seawater fish highly-processed product, which comprises the following steps: unfreezing iced fresh seawater fish by running water, taking the fish, rinsing the fish by deionized water for one to three times, and blending to obtain fish paste; sequentially adding the following components in percentage by weight: 2.5% of table salt, 10% of cassava starch, 1-10% of pigskin collagen and 2-5% of microbial fermentation dietary fiber, controlling system moisture to be 75-80%, and uniformly blending to prepare crude fish paste; performing gelatinization for 45-60 minutes at 30-40 DEG C, heating for 15-30 minutes at 90-100 DEG C, taking out the crude fish paste and putting the crude fish paste into ice water for rapid cooling to obtain the fish paste product. The preparation method is easy and feasible and suitable for industrial production; in addition, the quality of the fish paste product can be remarkably improved, the excellent freeze-thaw stability of dietary fiber ensures that the quick-freeze fish paste product has a longer high-quality shelf life, so that the commercial value of low-value seawater fish is improved.
Owner:ZHEJIANG UNIV
Sea fish mixed feed for deepwater net cage culture and manufacturing method thereof
InactiveCN103478497ARaise the ratioEmission reductionAnimal feeding stuffJuvenile fishAnimal Foraging
The invention relates to a sea fish mixed feed for deepwater net cage culture and a manufacturing method thereof, and belongs to the technical field of forage processing. The mixed feed comprises the following components by weight: 0-15 parts of fish meal, 50-70 parts of an enzymatic protein raw material, 5-12 parts of soybean meal, 8-14 parts of flour, 4-6 parts of squid visceral meal, 4-6 parts of fish oil, 1.5-2.5 parts of mineral premix, 0.8-1.2 parts of vitamin premix, 0.3 -0.7 part of choline chloride, 0.5-1.5 parts of calcium dihydrogen phosphate, 0.5-1 part of coated lysine, 0.2-0.5 part of coated methionine, 0.4-0.8 part of an adhesive. The processing steps are as below: 1) preparing an enzymatic hydrolysis protein raw material; 2) preparing materials; 3) crushing; 4) mixing; 5) hardening and tempering, and granulating; 6) drying; and 7) cooling. The invention ha scientific formula and reasonable technology; and the forage can satisfy nutrition for juvenile fish growth, has excellent stability, durability and sedimentation velocity, is convenient for eating by fish, realizes high utilization rate and reduces environmental pollution.
Owner:山东升索饲料科技有限公司
Separation and purification method of mung bean trypsin inhibitor and its application in the production of surimi products
ActiveCN102295700AEasy to separateReduce volumePeptide preparation methodsProtease inhibitorsPurification methodsDesorption
The invention discloses a separation and purification method of an MBTI. The method comprises the following steps: 1, extraction; 2, neutralization; 3, heat treatment; 4, membrane treatment; and 5, adsorptive separation, and desorption. So the high purity MBTI is obtained. The invention also discloses an application of the MBTI, and the application is that the MBTI, which is added to surimi products treating seawater fish or freshwater fish as a main raw material and is uniformly mixed, is used for enhancing the elasticity of the surimi products. The separation and purification method of the invention has the advantages of simple technology and good separation effect, and the MBTI has a great improvement effect on the elasticity enhancement of the surimi products.
Owner:JIMEI UNIV
Method for processing pickled seawater fish with grain and wine
ActiveCN104000237AImprove qualityImprove pickling efficiencyFood ingredient as antioxidantFood ultrasonic treatmentAlkaline waterPhenolic content in tea
The invention provides a method for processing pickled seawater fish with grain and wine. The method includes the following steps of pretreatment of seawater fish, cleaning, combination of alkaline water treatment and ultrasonic treatment for fishy smell elimination, pickling, dehydration, pickling with grain and wine, vacuum packaging and disinfection. The fishy smell and redundant fat of seawater fish are eliminated through alkaline water and ultrasonic waves, so that the product quality is improved, wherein the alkaline water is obtained through electrolysis. A vacuum pickling mode is adopted, so that pickling efficiency is greatly improved. The seawater fish is soaked and treated in a ClO2 solution, so that the good disinfection and preservative effect is achieved. The seawater fish is coated with natural antioxidant tea polyphenol, so that the good bacteriostat preservation effect is achieved.
Owner:福建坤兴海洋股份有限公司
Preparation method of microcapsule feed smaller than or equal to 1.8cm for rhubarb fry
InactiveCN102987149AIncrease profitIncrease attractivenessClimate change adaptationAnimal feeding stuffBiotechnologyAnimal science
The invention discloses a preparation method of microcapsule feed smaller than or equal to 1.8cm for rhubarb fry, which comprises the following steps: (I) the core material of the formula comprises imported fish meal, bean pulp, rapeseed meal, cuttlefish liver paste, flour and mineral substances, and the wall material of the formula comprises spirulina, fish oil, vitamins, gelatin and water; (II) the core material of the formula is subjected to puffing pretreatment and superfine grinding; (III) the wall material of the formula is homogenized through a colloid mill; (IV) microcapsule bait is prepared by a spray drying method; (V) the proportion of the wall material coating to the core material is (1-10):100; and (VI) the ratio of vitamin C polyphosphate to a chitosan solution for secondary coating is (2-10):100. Experiments prove that the main raw material adopted by the invention is easy to digest and absorb since the microcapsule bait prepared by puffing pretreatment and microcapsule granulation has good food attraction; and moreover, the secondary coating technology can keep the stability and suspension property of bait in water more effectively, thus a broad application prospect of the rhubarb fry initial feed in seawater fish feed is developed.
Owner:浙江恒兴饲料有限公司
Vitamin feed additive premix for sea fish
InactiveCN103039724AImprove the body's immunityNo adverse effects on palatabilityAnimal feeding stuffPhosphateVitamin B6 synthesis
The invention relates to a vitamin feed additive premix for sea fish, and belongs to the technical field of feed additives for fish culture. The vitamin feed additive premix is prepared by uniformly mixing vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B 12, nicotinic acid, folic acid, calcium pantothenate, biotin, inositol, VC (vitamin C) phosphate ester and zeolite powder according to a certain percentage ratio and further adding into feed according to the proportion of 0.5%-1%. The vitamin feed additive premix disclosed by the invention has the following advantages: (1) the vitamin feed additive premix can be used during the whole culture cycle; (2) the adding amount in the feed for the sea fish is 0.5%-1%; (3) the immunity of fresh water fish can be effectively enhanced and the survival rate is improved; (4) the vitamin feed additive premix disclosed by the invention has no adverse effects on palatability of the feed; and (5) raw material sources used by the vitamin feed additive premix disclosed by the invention are stable, the price is low, and the production process is simple.
Owner:青岛玛斯特生物技术有限公司
Microecological preparation for improving growth performance of pampus argenteus juvenile fish and application of microecological preparation
InactiveCN105192308AGain weight fastIncrease specific growth rateAnimal feeding stuffSeawaterFishery
The invention relates to a microecological preparation applicable to pampus argenteus juvenile fish and application of the microecological preparation, and belongs to the technology of seawater fish type feed additive application. The invention aims at selecting probiotics applicable to the pampus argenteus juvenile fish for thus improving the growth performance of the pampus argenteus juvenile fish. By comparing and analyzing the safety and the beneficial function of bacillus subtilis, lactobacillus plantarum and clostridium butyricum, the condition that the bacillus subtilis does not have any harm to the pampus argenteus juvenile fish and has the best beneficial effect is found. The growth performance and the immunologic function of the pampus argenteus juvenile fish are obviously improved by the bacillus subtilis, so that the microecological preparation is recommended to be used in artificial culture of pampus argenteus.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Wild domestication system and domestication method for proliferation and release marine fish fries
ActiveCN112753635AIncrease Feral RecoveryImprove survivabilityData processing applicationsClimate change adaptationMarine fishPredation
The invention provides a wild domestication system and domestication method for proliferation and release marine fish fries, and belongs to the technical field of marine biological resource conservation. The domestication system comprises a domestication field, a light source, a flow making pump, an artificial fish reef and a camera system. The system provided by the invention is used for domesticating the ingestion and predation escape capability of the release fries, enhancing the capability of the artificially propagated proliferation and release fries to adapt to the marine natural environment, improving the survival rate of the release fries, and further improving the proliferation effect of seawater fish release.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +1
Method for artificially culturing perineries aibuhitensis in biological filter
The invention discloses a method for artificially culturing perineries aibuhitensis in a biological filter. The method is mainly characterized in that: a gravel layer, a coarse sand layer, a fine sand layer and a soil layer are sequentially spread from bottom to top in the filter provided with a water inlet pipe and a drain pipe. Before clamworm seedlings are put into the filter, fresh water is injected into the whole filter, the filter is soaked for 1 week, the fresh water is drained after 1 week, and fresh seawater is injected. The perineries aibuhitensis seedlings are artificially scattered, the size of the seedlings is 3 to 10 joints, and the seedling density is 600 to 2,000 pieces per square meter. The quantity of bait is 8 to 12 percent of the overall weight of the perineries aibuhitensis in the filter, and the bait consists of residual bait and feces of artificially cultured seawater fish, wherein the residual bait and feces are filtered and collected by a silk net of 50 to 100 meshes and concentrated and dried. The temperature of the perineries aibuhitensis culture water in the filter is 4 to 40 DEG C; in 1 to 2 weeks after the seedlings are put, water is not drained, and only water is injected; and after 1 to 2 weeks of putting the seedlings, the water is changed twice every day, and the water in a volume which is 1 / 3 of the volume of the filter water is artificially changed every time. By the method, the perineries aibuhitensis with high survival rate can be cultured artificially in batch.
Owner:DALIAN OCEAN UNIV +1
Technique for extracting anti-oxidant from low value sea water fish and leftover protein
InactiveCN101233950AReduce manufacturing costRealize high-value development and high utilizationFood preservationAnimal proteins working-upUltrafiltrationChemistry
The invention discloses a technology for extracting antioxidant from the protein of low value marine fishes and the scraps thereof after processing. The materials, low value marine fishes and the scraps thereof after processing, are added with water with a weight 2 to 4 times of the materials and are ground into a meat pulp with a pH value regulated to 5 to 7; pawpaw protease and flavorzyme in a ratio of 1 : 1 is added hydrolyzed and enzyme quantity is controlled between 600 to 2,400 IU; the enzymolysis liquid is filtered and the pH value of the obtained ultrafiltration hydrolysate is regulated to 6 to 8; the regulated ultrafiltration hydrolysate is continuously stirred; 1 portion of ferrous chloride or calcium chloride with a concentration of 1 to 4 percent are added in 50 portions of ultrafiltration hydrolysate according to a weight / volume proportion and then the mixed solution is chelated, centrifuged to obtain sedimentation A; absolute alcohol is added in the filtrate until the concentration is 50 to 60 percent and the obtained filtrate is silenced and centrifuged to obtain sedimentation B; alcohol is added in the filtrate until the final concentration is 80 percent and the obtained filtrate is silenced and centrifuged to obtain sedimentation C; the sedimentations A, B and C are dried in vacuum so as to obtain antimicrobial; the antioxidant activity of the antimicrobial reaches 57.1 to 94.4 percent of that of Vitamine E; besides, the antimicrobial can be used in foods industry and relevant industry.
Owner:ZHEJIANG OCEAN UNIV
Artificial breeding method for sillago sihama
The invention relates to an artificial breeding method for sillago sihama, belonging to the technical field of artificial breeding of fishes. The method taking more than 100g of adult sillago sihama as parent fishes comprises the following steps of: carrying out antenatal nutrient enrichment and parent fish environmental control on the parent fish to promote the parent fishes to naturally lay eggs so as to obtain germ cells, carrying out artificially-regulated incubation and artificially-regulated larvae and young fish cultivation on the obtained germ cells, and carrying out nutrient enrichment and feeding on series of bait in the period of opening and seedling cultivating. According to the method, the enriched cultivation of the wild sillago sihama parent fish is solved, the nature spawning of the wild sillago sihama parent fish is promoted, and the artificial breeding technology of the germ cell incubation, the seedlings and the parent fish is artificially regulated, so that the condition can be created for the large-scale cultivation of the sillago sihama; and the new variety can be increased for the artificial culture and proliferation of marine fishes, and the artificial breeding method plays a very important role in the natural resource recovery and industrial development of sillago sihama.
Owner:珠海德洋水产养殖有限公司
Cultivation process of converting freshwater fish into salt-water fish
ActiveCN101019515ADelicious meatClimate change adaptationPisciculture and aquariaWater circulationSaltwater fish
The present invention relates to fish cultivation, and is especially cultivation process and special apparatus for converting freshwater fish into saltwater fish. The special apparatus consists of fish pond, dirt intercepting box, water pump, protein decomposing tower, air mixer, waste disposing pipe, filtering box, ultraviolet sterilizer, power supply regulator, etc. The cultivation process includes indoor cultivation, water circulation, four stage salting, four stage filtering, ultraviolet sterilizing, and throwing microbe seed for propagating fish feed. The present invention is one green cultivation process and the produced fish is excellent green food.
Owner:广州石渚农业发展有限公司
Preparation method for subunit vaccines of Cryptocaryon irritans and application of subunit vaccines of Cryptocaryon irritans
The invention relates to a preparation method for subunit vaccines of Cryptocaryon irritans and application of the subunit vaccines of the Cryptocaryon irritans. Approval application and industrialization of the whole organism vaccines of the Cryptocaryon irritans encounter a great challenge, mostly because the parasites cannot be cultured in vitro and only breed on fish bodies, enough Cryptocaryon irritans bodies for producing the vaccines are difficult to obtain, most importantly, the normative production process and the quality standard are difficult to make, and thus, people are forced to conduct research in a deeper level to develop genetic engineering vaccines. Candidate genes of surface proteins C1 are screened from a transcriptome database of the Cryptocaryon irritans, and recombination proteins are expressed on tetrahymena, and accordingly the vaccines are prepared for immunizing seawater fishes; and by detecting the immunogenicity and protectiveness of the vaccines, effective protective antigenic genes of the Cryptocaryon irritans are screened.
Owner:SOUTH CHINA AGRI UNIV
Anguilla anguilla liver cell line and construction method and application thereof
InactiveCN104531608ABroad application spaceSimple technical solutionMicroorganism based processesArtificial cell constructsImmune resistanceMicrobiology
The invention discloses an anguilla anguilla liver cell line and a construction method and application thereof, and belongs to the technical field of sea fish cell culture. The cell line is preserved in the Chinese typical culture preservation center on December 16, 2014, and the serial number of preservation registration is CCTCC NO: C2014239. The anguilla anguilla liver cell line is fibroblast cells, the continuous passage achieves more than 60 generations, the adherence after cryopreservation resuscitation is good, the growth is stable, the liver cell line can be directly used for research on aquatic virology, anguilla anguilla cell biological characteristics and immune resistance gene function, and the lever cell line has been proved to be sensitive to eels herpes virus. According to the anguilla anguilla liver cell line and the construction method and application thereof, the constructing method is simple and convenient to implement, the repeatability is high, and the cost is moderate. The anguilla anguilla liver cell line and the construction method and application thereof can also be applied to the preparation of other anguilla anguilla cell lines.
Owner:BIOLOGICAL TECH INST OF FUJIAN ACADEMY OF AGRI SCI
Application of bracteose eucalyptus essential oil and complex bacterial agent thereof in preparing insect repellent for marine fishes
ActiveCN110537504ADoes not affect food intakeDoes not affect growthClimate change adaptationAnimal feeding stuffMarine fishInsect repellent
The invention provides application of bracteose eucalyptus essential oil and a complex bacterial agent thereof in preparing an insect repellent for marine fishes. The bracteose eucalyptus essential oil complex bacterial agent comprises the bracteose eucalyptus essential oil and streptomyces griseus HL-50 of which the bacterial content is not smaller than 5*10<8> CFU / g, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No.18424, and the primary effective constituent of the bracteose eucalyptus essential oil is 1,8-eucalyptol. The bracteose eucalyptus essential oil complex bacterial agent is easy to prepare and use, and the effects of effectively expelling various parasites which infect the marine fishes and inhibiting bacteria can be achieved simply by uniformly mixing the bracteose eucalyptus essential oil complex bacterial agent in edible feed for the marine fishes according to the use amount of 0.5-1.5 kg per ton of the feed and continuously feeding the fishes with the feed for 4-7 days; moreover, the complex bacterial agent is poisonless, harmless, and suitable for being used by the marine fishes for a long term, reduces the breeding cost, and has a wideapplication prospect.
Owner:SHANDONG LONGCHANG ANIMAL HEALTH PROD
Mariculture fish multiple anti-idiotypic egg yolk antibody vaccine and preparation method thereof
ActiveCN108003240AImprove the immunityEasy to useAntibacterial agentsEgg immunoglobulinsVibrio alginolyticusSeawater
The invention provides a preparation method of a mariculture fish multiple anti-idiotypic egg yolk antibody vaccine. The preparation method comprises the following steps of after inactivating mariculture fish pathogenic bacteria, immunizing mice and rabbits; after using six rabbit anti-pathogenic serums to be mixed with the inactivated mariculture fish pathogenic bacteria, immunizing laying hens;and screening the laying hens, collecting, refining and purifying an obtained coarse anti-idiotypic egg yolk antibody and the mariculture fish multiple anti-idiotypic egg yolk antibody vaccine, wherein the mariculture fish pathogenic bacteria are vibrio anguillarum, Edwardsiella tarda, vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus and streptococcus. The multiple anti-idiotypic egg yolk antibody vaccine provided by the invention can strengthen the resistance of marine fishes to various fish diseases, can prevent various marine fish diseases, can also perform immunization to the marine fishes through an oral way, is simple, convenient and feasible and is liable in obtaining materials at the aspect of preparing an anti-idiotypic egg yolk antibody, is convenient with aseptictreatment, is simple and convenient in preparation programs, low in cost and high in yield and accords with national policy orientation of energy conservation, environmental protection and sustainable development.
Owner:西安德轩驰生物科技有限公司
Cheese shrimp paste and processing method thereof
ActiveCN108497365ARich in nutrientsGreat tasteFood ingredient as anti-freezing agentFood ingredient as mouthfeel improving agentBiotechnologyWater Chestnuts
The invention discloses cheese shrimp paste. The cheese shrimp paste is prepared from the following raw materials in parts by mass: 50-80 parts of shrimp meat, 20-40 parts of fish meat, 5-10 parts ofhigh-melting point cheese, 2-5 parts of skimmed milk powder, 5-10 parts of water, 5-10 parts of frozen egg white, 1-2 parts of cassava flour, 1-2 parts of water chestnut starch, 0.5-1 part of soybeanmeal, 0.5-2 parts of corn starch, 0.5-2 parts of sweet potato starch, 0.5-1.5 parts of table salt, 0.5-1 part of soy sauce, 2-4 parts of sucrose, 1-2 parts of Shaoxing aged wine and 1-1.5 parts of chicken essence. A preparation method comprises the following steps: granulating live shrimps, granulating two kinds of sea fish, granulating the cheese, adding mixed starch and seasoning to the shrimp granules, fish meat granules and cheese granules according to certain parts by mass, performing uniform stirring and mixing, soaking the mixture in an anti-freezing agent for 3-10 seconds and taking out the mixture; and putting the mixture into plates, and performing freezing forming and packaging. The preparation method is simple and the prepared shrimp paste is smooth in taste, rich in nutrientsand easy to preserve.
Owner:WENZHOU VOCATIONAL COLLEGE OF SCI & TECH +1
Compound feed for improving muscle quality of fugu rubripes
The invention discloses compound feed for improving muscle quality of fugu rubripes, amd belongs to the field of marine fish compound feed. The formula of the compound feed comprises the following rawmaterials by mass percent: 42 to 48% of white fish meal; 4 to 5% of yeast; 4 to 6% of krill powder; 4 to 5% vital gluten; 3 to 5% of corn gluten meal; 6 to 8% of soybean meal; 13.1 to 14.5% of wheatflour; 5.5 to 6% of fish oil; 1.5% of phospholipid; 1.5% of calcium dihydrogen phosphate; 1% of vitamin mixture; 1% of mineral mixture; 4.8 to 6.4% of functional additive. Compared with fresh trash fish, the special compound feed disclosed by the invention significantly improves the growth, muscle nutritional value and muscle quality of the fugu rubripes under the urban aquaculture conditions, reduces the dependence on the fresh trash fish in the aquaculture production, and reduces the waste of wild fishery resources and pollution of the cultured marine environment.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Breeding method for increasing survival rate of fish larvae
ActiveCN105830962AImprove survival rateSolve the bottleneck problem of cultivationClimate change adaptationAnimal feeding stuffAnimal scienceFish larvae
Owner:海南永贺生物科技有限公司
Method for preparing inactivated vaccine of anti-Listonella-anguillarum egg yolk antibody
InactiveCN102836431AImprove immunityImprove survival rateAntibacterial agentsAntibody ingredientsBiotechnologyDisease
The invention relates to a method for preparing inactivated vaccine of anti-Listonella-anguillarum egg yolk antibody. The method comprises the following steps: inactivating and centrifuging etiology Listonella-anguillarum BH1 so as to obtain inactivated thallus; preparing Astragalus mongholicus leachate; preparing oil phase by white oil, aluminium stearate and span-80; preparing vaccine water phase by the inactivated thallus and the Astragalus mongholicus leachat; finally, mixing the oil phase with the water phase according to the volume ratio of 2:1, emulsifying and milling for 5 minutes so as to obtain the Vibrio parahaemolyticus oil emulsion inactivated vaccine in which Astragalus mongholicus and oil white are used as united adjuvant. By adoption of the vaccine, the anti-Listonella-anguillarum egg yolk antibody can be obtained; by adoption of the anti-Listonella-anguillarum egg yolk antibody, the immunity of marine fishes such as cynoglossus semilaevis to etiology Listonella-anguillarum is improved, so that the survival rate is improved, the yield is improved, and the economic benefit of culture production of marine fishes such as cynoglossus semilaevis is improved. The problem that the Listonella-anguillarum caused diseases frequently occur in the culture production of marine fishes such as cynoglossus semilaevis, is solved.
Owner:HUAIHAI INST OF TECH
In vitro culture method for scatophagus argus kidney cells
InactiveCN102634481AThe culture method is reproducible and strongGood effectVertebrate cellsArtificial cell constructsPhysiologyScatophagus argus
The invention relates to an in vitro culture method for scatophagus argus kidney cells. The culture method comprises the following steps of: (1) preparing an L-15 basic culture medium, a primary culture medium, a basic fibroblast growth factor (bFGF)-containing primary culture medium and a subculture medium; (2) performing primary culture on the scatophagus argus kidney cells: a, acquiring a sterile kidney, b, digesting and separating the kidney cells, and c, inoculating; and (3) performing subculture on the scatophagus argus kidney cells. According to the invention, the adopted scatophagus argus kidney cell culture method is strong in repeatability and good in effect; and by reference and improvement on the conventional saltwater fish cell culture method, the culture method suitable for scatophagus argus kidney cell culture is groped, a stable kidney cell culture system is established, a scatophagus argus kidney cell line is established for the first time, a cell model is expected to provide for osmotic pressure regulation of scatophagus argus, and deep research of functional genomes of the scatophagus argus is facilitated.
Owner:SHANGHAI OCEAN UNIV
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