31 results about "Saltwater fish" patented technology

The invention discloses a fish and shellfish symbiosis seaborne net cage culture system and method, and relates to the technical field of aquaculture, and the method comprises the following steps: arranging a culture net cage in an offshore sea area; fish fries are put into a surrounding net, bivalve shellfishes are put into a shellfish culture piece cage, and the shellfish culture piece cage is hung in the culture net cage; feeding bait irregularly, checking the integrity of the shellfish culture cage and the covering condition of attachments regularly, and making a treatment means according to the covering rate; in the later period of culture, the adult fishes with the body length exceeding a preset value are caught irregularly in a fishing mode, and after the culture period is finished, the remaining adult fishes are caught in a concentrated mode by increasing the height of the purse seine; the shellfish culture piece cage is lifted out of the water surface to collect adult shellfish. The culture system disclosed by the invention comprises a culture net cage and shellfish culture piece cages, wherein the shellfish culture piece cages are hung in a purse seine. According to the method, mutualism of marine fishes and bivalve shellfish can be achieved, the resource utilization rate and the culture benefits are improved, and diversified fishery development can be achieved.

The utility model relates to a marine fish large-scale fry breeding incubation net cage, which comprises at least one incubation net cage, each incubation net cage comprises an outer cage body, an isolation flexible film is arranged at the position 1 / 3 away from the bottom in the outer cage body, a net bag is arranged in the outer cage body above the isolation flexible film, a through rotating main shaft is arranged in the outer cage body below the isolation flexible film, and the rotating main shaft is connected with the isolation flexible film. Fan blades are fixed on the rotating main shaft at intervals, the fan blades are oval, the rotating main shaft is located at the non-midpoint position of the long axis of the fan blades, and every two adjacent fan blades are symmetrically distributed with the rotating main shaft as the axis; one end of the rotating main shaft is connected with a transmission assembly; and the other end of the rotating main shaft extends into the adjacent hatching net cage. The fan blades which rotate along with the rotating main shaft to alternately jack up different positions of the bottom of the net bag are arranged at the bottom of the hatching net cage, so that roes deposited at the bottom of the net bag are turned over, oxygen deficit of the roes is avoided, the number of the roes placed in a single net cage can be properly increased, and the hatching scale is increased.

The application discloses a kind of mild extraction observation counting device and method of marine fish fry, and device includes sequentially connected suction line, observation platform, weak fry sorting device and overflow drain pipe.Suction line is equipped with variable frequency negative pressure pump and transparent steel wire hose;Observation platform is equipped with multistage slow-flow buffer structure inside, and observation area and shooting fixed mechanism are equipped in side and top;Weak fry sorting device is equipped with cyclone flow guide structure inside, and form rotating flow field, so that weak fry is detained in central slow-flow area and is discharged through bottom discharge port, and healthy fry is discharged from top with outside water flow;Overflow drain pipe inlet is bent upwards, and automatic overflow drainage is realized.The application integrates fry mild suction, dynamic counting, cyclone weak fry elimination, automatic drainage and raw water replacement, has no exposed metal, high waterproof level, adapts to seawater environment, significantly reduces fry damage rate and labor cost, and is especially suitable for transportation and screening of marine fish fry such as Epinephelus, Pseudosciaena crocea and the like.

This invention relates to the field of aquatic compound feed technology, specifically to a special feed for mullet, silver carp, and golden carp cultured in saline-alkali land in Northwest China. The feed is characterized by being composed of the following raw materials by weight percentage: 10%–14% alfalfa meal, 16%–22% soybean meal, 16%–22% corn meal, 5%–7% molasses, 1%–3% yeast powder, 2%–5% lactic acid bacteria preparation, 2%–5% premixed feed for aquatic life in saline-alkali land in Northwest China, 1%–4% fish oil, and 1%–5% vegetable oil. This invention solves the problems of low survival rate, high feed conversion ratio, easy antagonistic inactivation of probiotics, fish oil oxidation, poor water resistance of pellets, and insufficient regional adaptability of traditional feeds. Compared with commercially available ordinary marine fish feeds, the survival rate of juvenile fish is increased by 15% to 30%, the feed conversion ratio is reduced by 10% to 20%, the Vibrio inhibition rate is ≥80%, the power consumption per ton of feed is reduced by 25% to 35%, and the raw material cost is reduced by 30% to 45%. It is suitable for large-scale aquaculture in saline-alkali land in Northwest China and has important value for improving the quality and efficiency of fisheries, ecological restoration, and rural revitalization.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

The application provides a turbot compound immune enhancer, a preparation method and application thereof. The application discloses application of a composition containing a series of components in preparation of a turbot immune enhancer, and the immune enhancer can effectively improve expression of a proinflammatory related gene of the turbot, reduce a number of bacteria in a liver or an intestinal tract, enhance a bactericidal capacity of the turbot, enhance an antioxidant stress capacity of the turbot, relieve liver damage of the turbot after being infected by bacteria, and relieve tissue damage of the turbot after being infected by bacteria. The immune enhancer component is simple, environment-friendly, low in cost and ideal in effect, and provides a new scheme for improving a breeding level of marine fish and improving a survival rate.

The present application belongs to the technical field of compound feed for marine fish, and particularly relates to a feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii and a preparation method thereof. The feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii is prepared from the following raw materials in mass percentage: 95% to 96.5% of basic ingredients, 1% of compound vitamins, 1% of compound mineral salts, and 1.5% to 3% of an antioxidant enzyme activity composition, and the sum of the mass percentages of the raw materials is 100%; the antioxidant enzyme activity composition is composed of vitamin C and beta-carotene, and the mass ratio of vitamin C to beta-carotene is 1 to 3: 1 to 3. The feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii obtained by the present application not only can provide sufficient natural antioxidant components to enhance the antioxidant defense mechanism of the juvenile fish, but also can effectively solve the problems of slow growth, low feed utilization rate, and water pollution of juvenile Hexagrammos otakii caused by insufficient antioxidant activity.

The utility model relates to fish product processing technical field especially, it is a kind of sea fish product frying feeding device, it is thawed and is conveyed to frozen fish product by operation platform and first conveying device cooperation, fish product surface is dried by drying device, fish product after drying is conveyed by second conveying device, fish product surface is wrapped powder operation by powder supply device, and fish product surface excess flour is vibrated and removed by vibration device;Including operation platform;Still including first conveying device, drying device, second conveying device, powder supply device and vibration device, first conveying device, drying device, second conveying device, powder supply device and vibration device are all installed on operation platform;First conveying device and second conveying device are conveyed, drying device is dried, powder supply device is surface powder spraying, and vibration device is vibrated.

This application discloses a safety protection structure for transporting marine fish, relating to the field of seafood transport technology. It includes a belt, a support frame, two drive rollers, and a drive motor. The support frame supports the two drive rollers, which are rotatably connected to the support frame. The belt passes around the two drive rollers sequentially. The drive motor drives the drive rollers to rotate. The belt surface is provided with several magnetic strips, which are sleeved on the outside of the belt and arranged parallel to the belt width. The drive roller at the belt output end is a magnetic roller. A support plate is fixed inside the support frame, located between the two drive rollers and inside the belt. A permanent magnet is fixed to the lower end of the support plate near the magnetic roller, and an electromagnet is fixed to the side of the permanent magnet away from the drive roller. A collection component is provided at the lower end of the support frame for collecting metallic impurities. This application improves the ease of separating marine fish from impurities.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

This invention belongs to the field of formulated feed technology for marine fish, specifically relating to a feed for improving the muscle quality of *Hexagrammos oysterfish* and its preparation method. The feed for improving the muscle quality of *Hexagrammos oysterfish* is made from the following raw materials in the indicated weight percentages: 93.5%–96.5% basic ingredients, 1% compound vitamins, 1% compound mineral salts, and 1.5%–4.5% formulated feed composition, with the sum of the weight percentages of all raw materials being 100%. The formulated feed composition consists of yeast β-glucan, chitosan oligosaccharide, and coated sodium butyrate, with the mass ratio of yeast β-glucan, chitosan oligosaccharide, and coated sodium butyrate being 1–3:1–3:1–2. This invention not only effectively reduces fat deposition in the body of *Hexagrammos oysterfish*, improving its muscle elasticity and chewiness, but also significantly improves the textural properties of the fish meat, thereby enhancing the overall muscle quality of *Hexagrammos oysterfish* and meeting consumer preferences.

The utility model discloses a kind of vacuum density meters for aquatic feed, it is related to aquatic extruded material production equipment technical field, including placing base. The upper surface of the placing base is equipped with extruded cylinder, and the end of extruded cylinder is equipped with servo motor one, and servo motor one output screw rod rotates. The vacuum density meter for aquatic feed, compared with the existing ordinary aquatic extruded material density control instrument, cutting tool and pressure chamber are designed as a whole by connecting cylinder, reduce intermediate connecting component, optimize internal structure by finite element analysis, select high-strength, corrosion-resistant alloy material, ensure stability and sealing under high-pressure environment, to produce % sink water material, ensure that equipment can be in seawater fish material and crayfish material production environment Long-term stable operation;Adopt high-precision laser cutting and numerical control bending technology to improve part accuracy and assembly stability, improve equipment surface protection ability through high-quality painting treatment, adapt to humid and strong corrosion environment.

This utility model discloses a device for measuring the growth indicators of marine fish, specifically a base plate, and further includes: two sets of weighing components for measuring the weight of marine fish; an adjusting component for adjusting the distance between the two sets of weighing components, and the adjusting component is located on the top of the base plate; and a fixing component for fixing the marine fish, and the fixing component is located on the weighing components. The adjusting component includes side plates, which are fixedly installed on both sides of the top of the base plate. A screw is rotatably connected between the two sets of side plates via bearings, and the thread directions at both ends of the screw are opposite from the middle. Both ends of the screw are threadedly connected to sliders. This utility model, by setting a fixing component on the weighing components, enables the fixing of marine fish during the measurement of their growth indicators, thereby improving the accuracy of weighing the marine fish.

The invention discloses a marine fish bait and a preparation process thereof, and relates to the technical field of breeding feeds, the marine fish bait comprises the following components in parts by weight: 73.2-82.8 parts of a protein component, 10.8-13.2 parts of an energy component, 4.5-5.6 parts of a strengthening component, 2.5-3.5 parts of a functional additive and a compound bacteria solution accounting for 33.5-40% of the total mass; wherein the protein component is prepared from 28 to 32 parts of brown fishmeal, 40 to 43 parts of modified compound protein, 4 to 6 parts of soybean protein concentrate and 1.2 to 1.8 parts of compound enzymolysis fish soluble pulp; the modified compound protein is amphiphilic compound modified protein grafted with a hydrophilic monomer and a hydrophobic monomer. Through combination of the amphiphilic compound modified protein enhanced components and synergistic effect of the specific components, the effects of mildly dissolving the bait in seawater, improving the nutrient utilization rate and digestibility, reducing fish meal dependence and culture cost and assisting fish growth are achieved.

A high-survival-rate cultivation method for Cephalopholis sonnerati fry, relating to the technical field of marine fish cultivation. The cultivation method comprises the following steps: (1) regulation of a light source environment: during growth of Cephalopholis sonnerati fry, using a blue light source having a wavelength range of 420-450 nm to irradiate for 60-75 d; (2) modification of a water bottom environment: laying a layer of artificial material on a water bottom, introducing water having a salinity of 30-35‰ as cultivation water, and using an aeration pump for continuous aeration; and (3) alternate administration of feed A and feed B: first administering feed A for 8-10 d, then administering feed B for 8-10 d, and continuing alternate administration until the fry reach a juvenile stage, wherein feed A is prepared by adding Daphniphyllum calycinum Benth. to a base feed, and feed B is prepared by adding loperamide to the base feed. By means of three parallel measures of light source regulation + water bottom modification + alternate administration, it is beneficial to achieving high survival rate of Cephalopholis sonnerati fry, thereby improving economic benefits.

The utility model belongs to the technical field of marine fish culture, and particularly relates to a feeding device for marine fish culture, which comprises a culture fence, a moving mechanism and a feeding tank, the culture fence comprises a fence rod and a slide rail arranged at the top of the fence rod; the moving mechanism is arranged on the sliding rail and comprises a supporting frame, a double-shaft motor, a driving gear and a driven wheel, the double-shaft motor is arranged on the inner side of the supporting frame, the driving gear meshed with one side of the sliding rail is arranged at the bottom of the double-shaft motor, and the driven wheel making contact with the other side of the sliding rail is arranged at the bottom of the supporting frame; the feeding tank is arranged at the top of the supporting frame, the moving mechanism is arranged on the sliding rail, the driving gear is driven by the double-shaft motor to be meshed with the sliding rail for transmission, the supporting frame is driven to move circumferentially on the sliding rail, the feeding tank is arranged on the supporting frame, the feeding tank conducts feeding circumferentially on the breeding fence, the feeding range is expanded, and marine fishes are prevented from being collided and injured due to food grabbing.

The invention discloses a seawater fish and vegetable symbiotic zero-emission circulating planting and breeding system and method based on Bio-RAS, and belongs to the technical field of circulating agriculture, ecological engineering and facility agriculture. The system comprises a seawater culture unit, a biological enhancement water treatment unit, a plant water culture unit and a water circulation and supply unit which are sequentially connected through pipelines; the mariculture unit, the biological enhancement water treatment unit, the plant water culture unit and the water circulation and supply unit are all connected with the control system so that all the units can work cooperatively, and a substance and energy circulation chain of'cultivation-treatment-planting-backflow 'is formed. According to the method disclosed by the invention, zero liquid discharge and ultrahigh resource efficiency are really realized, 100% internal recycling of the breeding wastewater is realized through an efficient Bio-RAS treatment process, and the problem that the breeding tail water pollutes the environment is thoroughly solved.

The utility model discloses a kind of baiting devices for seawater fish culture, including base, the first spiral conveying pipe is arranged on the top of base, and the automatic feeding assembly is arranged on the side of base;Automatic feeding assembly includes feeding chute, the inside bottom longitudinal sliding of feeding chute is provided with support plate, and the bottom of feeding chute is provided with weighing plate, the utility model: bait is conveyed to support plate by spiral conveying pipe, and the weight of bait on support plate will be pressed on weighing plate, when reaching threshold value, spiral conveying pipe stops conveying, and electric valve closes, to ensure the precision of baiting amount, pusher frame and push plate are pushed by electric push rod, and push plate is pushed by spring, to ensure that push plate is always in contact with support plate, so that bait on support plate is pushed into water, to complete automatic feeding, by cyclic operation, i.e. without working person manually feeding, it is more convenient to operate.

A method for estimating the amount of marine fish red muscle which includes acquiring information on the fish's red muscle by operating a non-destructive inspection unit; and estimating the amount of the red muscle included in the fish body based on the acquired information by using a calculation unit. A method for farming a marine fish which includes determining an estimated value of an amount of a red muscle contained in a marine fish body, and adjusting a farming condition for the marine fish based on the estimated value.

This invention discloses a perilla compound water-retaining agent and its application in deodorizing and retaining water in aquatic products, belonging to the field of food processing technology. The water-retaining agent uses perilla extract as its core component, combined with compound cyclodextrin and 3,4-dimethoxy-3,5,7-trihydroxyflavone. The compound cyclodextrin is prepared from citrate monoglyceride compound cyclodextrin. Through single-factor experiments combined with electronic nose-assisted analysis, the optimal application process was determined using the fishy smell value and color difference value of marine fish as optimization indicators. When applied to marine fish processing, the phenolic substances in the perilla extract can inhibit lipid and protein oxidation, the compound cyclodextrin can specifically encapsulate fishy smell substances, and 3,4-dimethoxy-3,5,7-trihydroxyflavone jointly enhances water retention performance, achieving effective deodorization, significantly reducing the content of trimethylamine and nonenal, improving color stability, and optimizing processing quality. It is suitable for various aquatic product processing scenarios.

The invention discloses a deep learning-based seawater fish growth state prediction method and system, and belongs to the field of state prediction. According to the method, pixel-level time sequence motion features and sound source orientation time sequence features are extracted respectively through synchronously acquired underwater video streams and acoustic time frequency data, and then combined features are generated through weighted fusion of a cross-modal attention mechanism; and inputting the joint features into a cascade prediction model, sequentially performing noise suppression and information completion through a feature enhancement sub-network of a generative adversarial network architecture, performing processing through a growth prediction sub-network constrained by an embedded biological energy metabolism equation, and outputting a marine fish growth state prediction result. The problem that the growth state of the marine fish cannot be accurately and efficiently predicted in the prior art is effectively solved.

The present application belongs to the technical field of compound feed for marine fish, and particularly relates to a feed for enhancing the immune activity of Hexagrammos otakii and a preparation method thereof. The feed is prepared from the following raw materials in mass percentage: base ingredients 93.5-96.5%, compound vitamins 1%, compound mineral salts 1%, and compound feed composition 1.5-4.5%, with the sum of the mass percentages of the raw materials being 100%; the immune activity composition is prepared from dried orange peel, betaine, and yucca extract, and the mass ratio of the dried orange peel, betaine, and yucca extract is 1-3:1-3:1-2. The feed for enhancing the immune activity of Hexagrammos otakii provided by the present application, through the synergistic effect of dried orange peel, betaine, and yucca extract, not only optimizes the digestion process of Hexagrammos otakii, but also comprehensively improves the health level and immune activity of Hexagrammos otakii from multiple aspects of antibiosis, immune enhancement, and anti-inflammation.

The invention belongs to the technical field of fish genetic breeding and genetic engineering, and particularly relates to a rapid preparation method of a gene editing fish homozygous mutant based on a gynogenesis technology. According to the method, F0 or F1 generation female marine fishes such as paralichthys olivaceus obtained through gene editing operation serve as female parents, mature ova of the female marine fishes are collected, and the ova are activated through sperms subjected to genetic material inactivation treatment; then, chromosome doubling is induced in a cleavage inhibition mode, and the mitotic gynogenesis diploid is obtained. And further screening the filial generation obtained by the steps to obtain the mutant with homozygous genotype. The method is simple and convenient to operate, the period of obtaining the homozygous mutant can be remarkably shortened, the breeding efficiency is improved, and the method has a good application prospect.

The utility model relates to starch-free bait processing equipment for marine fishes, and aims to solve the problems that the conventional equipment cannot thoroughly crush raw materials, feed particles are not uniform and the operation is tedious. The equipment comprises a driving motor, a driving roller shaft, a spiral separating blade, a conical grinding roller and a conical sleeve. The driving motor drives the separation blade and the grinding roller to rotate through the driving roller shaft, the separation blade achieves primary smashing of raw materials and pushes the raw materials to the area of the grinding roller, the grinding roller and the conical sleeve form a gradually-reduced gap through the conical design and the gradient difference, the raw materials are further refined through extrusion and friction, and it is ensured that feed particles are uniform and fine. And through the connecting structure of the outer shell and the conical sleeve and the design of the arc-shaped surface, the raw material flowing path is optimized, and the machining efficiency is improved. A groove and a limiting ring in the conical sleeve enhance the equipment stability, and a limiting plate and a collecting end facilitate feed collection.

This invention provides a compound microecological preparation for enhancing the intestinal function of marine fish, its preparation method, and its application, belonging to the technical field of aquatic feed additives. The compound microecological preparation of this invention includes a carrier, traditional Chinese medicine extracts, and a compound bacterial flora; the traditional Chinese medicine extracts consist of seaweed extract, *Anemarrhena asphodeloides* extract, and *Eucommia ulmoides* leaf extract, with their total content accounting for 10-20% of the total weight of the compound microecological preparation; the compound bacterial flora consists of *Rhodopseudomonas palustris*, *Rhodotorula rubra*, and *Clostridium butyricum*, with a total viable count ≥15 billion CFU / g of the compound microecological preparation. This invention, through the scientific combination of traditional Chinese medicine extracts and the compound bacterial flora, effectively solves the intestinal health problems of marine fish, while simultaneously promoting growth and improving meat quality.

The present application relates to the technical field of seawater fish culture, and discloses a kind of seawater fish segmented culture dynamic monitoring and feeding optimization method.The method obtains underwater video stream and hydrology, water quality multi-parameter sensing data of different culture stages in multiple regions of culture pond, seawater net cage and deep sea culture platform, identifies fish group aggregation degree distribution atlas, extracts dissolved oxygen concentration gradient change curve and flow data;Fusion related parameter factor generates fish group behavior characteristic vector, calculates the feeding activity index, and dynamically corrects the preset seawater fish growth coefficient model;According to the model after correction generates feeding strategy control parameter, drives feeding equipment to execute differentiation area feeding.The method combines fish growth different stages fish group behavior and hydrology, water quality parameters, which helps to improve the accuracy of feeding, reduce feed waste, adapt to the growth needs of different regions of seawater fish at each stage, and is suitable for modern seawater fish culture scene.

This application discloses a method for controlling biogenic amines in the processing of marine fish, relating to the technical field of marine fish processing. The method includes thawing raw fish, pretreatment, biogenic amine control, and post-treatment. The main method for controlling biogenic amines involves first dripping antibacterial plant essential oil onto the surface of boneless fish fillets, and then soaking the fillets in a biogenic amine inhibitor (oregano essential oil-chitosan preservative solution) for 10-30 minutes. This application effectively improves the control of biogenic amines in marine fish. The volatile compounds in the plant essential oil have multiple functions, including antibacterial, insecticidal, and antiviral effects. When dripped onto the fish fillets, they can inhibit bacteria, and the essential oil flavor can reduce the fishy smell of marine fish. The subsequent soaking with the biogenic amine inhibitor reduces the formation of biogenic amines, effectively controlling their production and improving the overall control of biogenic amines in marine fish.

The invention belongs to the technical field of genetic engineering, and particularly relates to a grouper nervous necrosis virus yeast oral vaccine as well as a preparation method and application thereof. The invention firstly provides a saccharomyces cerevisiae engineering bacterium capable of efficiently expressing the RGNNV capsid protein, and the saccharomyces cerevisiae engineering bacterium comprises an RGNNV-CP gene cDNA sequence of which the nucleotide sequence is shown as SEQ ID NO.1 and which can efficiently express the RGNNV capsid protein, and a saccharomyces cerevisiae engineering bacterium capable of efficiently expressing the RGNNV capsid protein, on the basis, the invention further provides a grouper nervous necrosis virus yeast oral vaccine, the active ingredients of the oral vaccine comprise the saccharomyces cerevisiae engineering bacteria in the technical scheme, the vaccine is an oral dosage form aiming at fry, administration is convenient, professional equipment is not needed, stability is high, and storage and popularization are convenient; the method is especially suitable for artificial breeding requirements of marine fishes in tropical areas such as Hainan islands. Therefore, the technical scheme provided by the invention can be used for treating or preventing nervous necrosis viruses of groupers and improving the survival rate of fries.

The invention relates to a method, a system and a composition for delivering macromolecules to marine fish sperms based on exosomes, and belongs to the crossing field of biotechnology and reproductive engineering.According to the method, the exosomes displaying CD81 protein serve as a carrier, and the macromolecules are delivered to marine fish sperms through specific binding of the CD81 protein and a receptor ligand of CD9 protein on a fish sperm cell membrane. And delivering the biological macromolecules loaded in the exosome into the marine fish sperms. Targeting and tracing are integrated, the targeting can be enhanced through the potential interaction effect of CD81 and sperm surface protein CD9, and real-time tracing of the delivery process can be achieved through EGFP fluorescence.