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Practicalization method for frozen preserving sperm of fish

A fish sperm, cryopreservation technology, applied in the direction of preservation of meat/fish by freezing/cooling, etc., can solve the problems of inability to meet the requirements of sperm bank construction, unreported hatching rate of frozen fertilized fertilized fry, and small preservation, etc. Stable cryopreservation effect, stable frozen semen viability, and simple composition

Inactive Publication Date: 2005-03-23
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

In terms of foreign fish sperm cryopreservation, Dreanno et al. (1997) used 0.25ml straw as a cryopreservation container to carry out research on the cryopreservation of turbot sperm, but the fertilization rate of the obtained frozen sperm was only 58%. The hatching rate of fertilized fry, and the method reported in this paper has a small amount of preservation, which cannot meet the requirements of sperm bank construction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0015] One, take seawater fish turbot and perch semen cryopreservation as example, to the present invention

[0016] Embodiments will be described.

[0017] 1. Example of cryopreservation of turbot semen

[0018] (1) Gently press the abdomen of the mature male turbot, and at the same time collect the urine-free semen with a straw, and examine it under a microscope. The microscopic examination method is: use a toothpick to pick up a little sperm on a glass slide, then activate it with natural sea water, and observe it under a microscope immediately. The survival rate of spermatozoa is the percentage of motile spermatozoa in the microscopic view after semen activation to the total number of spermatozoa. Semen with a survival rate above 85% was used for the experiment.

[0019] (2) The collected turbot spermatozoa are directly diluted in the TS-2 containing 15% DMSO (volume ratio) pre-cooled at 4°C in a ratio of 1:2 (volume ratio), and the composition of TS-2 is: Tris- Cl 10m...

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PUM

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Abstract

A practical method for freeze storage of fish sperm features that the diluent, antifreezing agent and container for the freeze storage of fish sperm chosen from many schemes, and a three-step freezing mode and a quick thawing method are designed.

Description

Technical field: [0001] The invention belongs to the cryopreservation technology of animal sperm in cryobiology, and is a practical technical method for long-term preservation of fish sperm in an ultra-low temperature state (-196°C). Background technique: [0002] Germplasm resources are an important material basis for aquaculture production, breeding of fine varieties and sustainable development of aquaculture industry. my country is a country rich in aquatic organism germplasm resources. The rich and diverse aquatic organism germplasm resources and genetic diversity have played a very important role in the rapid development of my country's aquaculture industry. However, due to overfishing and disorderly utilization of fishery resources, some fish resources have declined and become endangered, such as Yangtze shad and Songjiang perch. If protection measures are not taken in time, it will be difficult to find many genetic resources of original and improved fish species in n...

Claims

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Application Information

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IPC IPC(8): A23B4/06
Inventor 陈松林季相山
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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