319 results about "Fish species" patented technology

Disclosed is a reservoir ecological operation method integrating ecological flow process and water temperature process requirements of fish species, including: establishing a habitat model for a target fish species and deducing an ecological flow process of a lift history of the target fish species; calculating a critical spawning water temperature threshold and a critical gonadal development accumulated temperature threshold; establishing a multi-objective reservoir operation model to obtain flow process and water level after optimized reservoir operation; establishing a multi-objective reservoir ecological operation model integrating both the ecological flow process and the water temperature process, and calculating reservoir outflow water temperatures before and after the optimized reservoir operation; and calculating an improvement difference between the critical water temperature threshold and the accumulated temperature threshold of the target fish species on arrival dates after the reservoir ecological operation and those before the optimized reservoir operation.

The invention discloses a multi-wave detection and imaging system for fish schools, which relates to the field of detection and imaging of fish schools and comprises a metamaterial acoustic emission module, a self-adaptive signal receiving module, a three-dimensional point cloud generation module, an image enhancement and generation module and an intelligent decision control module. According to the multi-wave detection and imaging system for the fish school, clustering parameters can be dynamically adjusted based on the real-time fish school density, the neighborhood radius is automatically expanded in a high-density area to avoid segmentation errors, and the core point judgment threshold is reduced in a low-density area to reduce missing detection; an acoustic attenuation model is constructed in combination with water body environment parameters, emission parameters are calibrated in real time, images can be enhanced, space and time dimension features are fused, the image resolution and definition are improved, accurate recognition of multiple fish species is achieved, the fish species can be better distinguished, meanwhile, a generative network is adopted to directly convert acoustic data into visual images, and the recognition accuracy is improved. Therefore, the generated image can reflect the actual distribution condition of the fish school more truly and accurately.

The invention discloses a device and a method for intelligently investigating types and quantity of fishes. The device comprises a self-adaptive sonar detection module, a three-dimensional image acquisition module, an image preprocessing module, a fish detection module, a main body segmentation module and an identification and classification module. The sonar module is integrated with a three-frequency-band transducer, can dynamically switch frequency according to fish school depth, and realizes target tracking counting and quantity estimation in combination with an algorithm; the image acquisition module constructs a fish school three-dimensional image model by combining a depth camera with a light attenuation compensation algorithm; the preprocessing module fuses acoustic and optical features and expands a data set; the detection and segmentation module is used for accurately positioning fishes and removing impurities; the identification and classification module realizes type identification based on transfer learning and supports incremental learning of new fingerlings. According to the method, through cooperation of multiple modules, the problems of insufficient detection precision, poor image quality and the like in traditional investigation are solved, efficient and accurate investigation of fish species and quantity is realized, and technical support is provided for fishery resource management and ecological protection.

The invention discloses a grouping ecological scheduling method capable of meeting different fish requirements to the maximum extent, and relates to the technical field of reservoir ecological scheduling, and the method comprises the steps: clearly researching the existing fish species of a river reach through environment DNA investigation; determining an overall framework and scheduling opportunity of ecological scheduling of the reservoir according to the spawning water temperature and spawning type grouping of each species; improving spawning habitat suitability evaluation, and defining an ex-warehouse flow range capable of maximally covering spawning of indigenous fishes in each regulation and control period; key indexes such as spawning stimulation and natural hydrological process recovery are comprehensively considered, and daily variation of ex-warehouse flow is optimized; and finally, coordinating ex-warehouse flow and water level change constraints of fishes of different spawning types to form a grouping and classifying ecological scheduling optimization scheme giving consideration to fish differentiation demands in different time periods.

The present invention belongs to the ecological field and provides a reservoir ecological regulation method that meets the requirements of fish spawning occurrence and fish egg density, including: obtaining the natural reproduction characteristic information and habitat information of fish species that produce drifting eggs in the regulated target river section, and determining the regulated target fish species; obtaining the first key habitat information required when the spawning occurrence probability of all fish species that produce drifting eggs and the regulated target fish species is not less than a first preset threshold; obtaining the second key habitat information required when the fish egg density of all fish species that produce drifting eggs and the regulated target fish species is not less than a second preset threshold; determining a reservoir ecological regulation plan according to the first key habitat information and the second key habitat information of the river section; and carrying out reservoir ecological regulation according to the reservoir ecological regulation plan. Through the above technical solution, the adverse impact of reservoir regulation operation on the spawning of fish species that produce drifting eggs can be alleviated, which is beneficial to promoting the restoration of fish resources and the protection of biodiversity.

The invention discloses a marine fish myoblast in-vitro proliferation method. A basal culture medium is used as a background culture medium of a culture medium; the culture medium contains IGF2 protein with the final concentration of 20-200 ng / mL; the IGF2 protein is derived from plectropomus leopardus. The culture medium is used for culturing the passage cells to obtain the myoblasts proliferated in vitro of the plectropomus leopardus, the in-vitro culture of the myoblasts of the plectropomus leopardus is successfully realized, the number of the myoblasts reaches 30000, and the in-vitro proliferation rate reaches 87%. The invention provides raw materials for exploring the self function of myoblasts and gene regulation and control mechanism, provides preconditions for an in-vitro certification experiment for further exploring the influence of the myoblasts of marine fishes on the growth speed, and also lays a foundation for improved variety breeding of the marine fishes.

The invention relates to the technical field of vehicles, in particular to a fish data processing method, device, medium and program based on machine learning, and the method comprises the steps: obtaining fish data and environment data of a target water area; extracting at least one fish morphological feature in the fish data and an environment feature of the environment data; the at least one fish morphological feature and the environment feature are input into a machine learning model, the machine learning model outputs a fish and environment matching result, and the machine learning model generates a fish morphological matrix based on the at least one fish morphological feature and generates an environment feature matrix based on the environment feature; determining a matching result of the fish and the environment according to the similarity of the fish form matrix and the environment feature matrix; and determining fishes matched with the target water area according to a matching result. Therefore, the problems of time consumption, labor consumption, low accuracy and the like due to the fact that the fishes cannot adapt to the current water area environment when the types of the fishes suitable for being cultured in the current water area are selected according to experience in related technologies are solved.

The invention belongs to the technical field of deep sea culture, and discloses a novel bottom-supported truss lifting folding intelligent culture net cage which comprises a bottom-supported truss platform, a net cage body is arranged in the middle of the bottom-supported truss platform, and a netting is arranged on the net cage body; the netting cover is arranged at the top of the net cage and used for sealing the top of the net cage; the connecting and moving device is arranged on the seat bottom type truss platform and the netting, the connecting and moving device comprises a netting bottom frame located on the outer side of the bottom end of the netting, and a movable frame is arranged at the top of the netting bottom frame; the net drawing frame is arranged on the top of the movable frame and the outer side of the top end of the netting. The truss base ascends and descends, the breeding depth can be better changed, the lifting assemblies are independently connected with the corresponding foldable net cages, nettings are prevented from being disordered, the multiple net cages can independently ascend and descend, then different fishes are bred and independently collected, applicability is high, the net cage utilization rate is increased, and modular breeding is facilitated.

The invention discloses a Pelteobagrus vachelli population dynamic monitoring system and method, particularly relates to the technical field of fishery resource monitoring, and aims to solve the problems of inaccurate juvenile fish survival rate evaluation and poor monitoring timeliness in a benthic hidden environment in the prior art. The method comprises the following steps: arranging a benthic penetration type sonar array to synchronously collect a water body reflection signal and a benthic environment sample, and extracting juvenile fish specific gene fragments by combining an environment DNA (Deoxyribose Nucleic Acid) enrichment technology to generate concentration distribution data; screening juvenile fish distribution data by using an acoustic reflection signal and body length correlation model, and positioning an active hot spot area through space-time overlapping analysis; deploying a directional sound wave excitation device to obtain escape trajectory data, and calculating a survival rate in combination with a density attenuation model; finally, multi-source data and topographic features are fused to generate a real-time population dynamic monitoring result, and high-precision and non-intrusive monitoring of the juvenile fish population in the hidden environment is realized.

The invention provides an intelligent measuring and calculating method for the high-density fish body length of a culture pond in the technical field of fish body length measuring and calculating. The method comprises the steps that S1, a large number of historical fish school images are acquired to train a fish detection model; s2, setting a fish form constraint parameter, calibrating a camera parameter, collecting a culture pond video through a binocular camera, and analyzing the culture pond video to obtain a left eye image and a right eye image; s3, inputting the left eye image and the right eye image into a fish detection model to obtain a fish body bounding box carrying confidence, and filtering the fish body bounding box through the fingerling form constraint parameters and the confidence; s4, performing stereo matching on the fish body bounding boxes based on the left eye image and the right eye image so as to calculate the depth value of the fish body corresponding to each fish body bounding box; and S5, obtaining the fish body length based on the fish body bounding box through the camera parameters and the depth value. The method has the advantages that the accuracy, efficiency and reliability of measuring and calculating the length of the fish body are greatly improved.

The invention discloses a method for monitoring, describing and diagnosing abnormal behaviors of fishes, which constructs an abnormal behavior feature extraction network perceived by using abundant branch aggregation pixels in a backbone network, and improves the feature representation capability of the backbone network for irregular body surface shape parts of fishes and complex contour targets of underwater fishes. A detail enhancement module of parallel pixel fusion attention is introduced into a hybrid encoder, important features of each sub-region are independently enhanced, and meanwhile, natural transition of features between adjacent blocks is ensured during sub-feature image fusion. And a cross-scale abnormal small target decoupling detection head with dynamic weight distribution is constructed in a decoder and a detection head module, so that the model can accurately detect abnormal targets with relatively deviated underwater shooting angles and immature and small-sized to-be-detected fingerlings. The method is suitable for rapidly capturing abnormal behavior characteristics such as fish rollover, rapid convulsion and illness, and successfully improves the speed and precision of fish abnormal behavior monitoring.

The invention provides a method for simulating proper spawning habitat distribution of settled fishes in a Tongjiang lake, and belongs to the technical field of ecological protection, and the method comprises the following steps: S1, selecting typical settled fish species in the Tongjiang lake as a research object; s2, investigating habitat factors influencing the spawning habitat, determining a suitable range of each habitat factor, and drawing a suitable degree curve of each habitat factor; s3, constructing a hydrodynamic model of the river-through lake area; s4, calculating a normalized vegetation index; and S5, according to a fuzzy logic method, taking each habitat factor and the normalized vegetation index as input, constructing a suitability simulation model of the suitable spawning habitat of the settled fishes in the Tongjiang lake, and calculating a comprehensive suitability index value of the spawning habitat. According to the method, factors such as water depth, flow velocity and vegetation coverage required by fish spawning are comprehensively considered, and the change condition of the comprehensive suitability of the settling fish spawning habitat in the Tongjiang lake can be simulated and quantified.

The invention provides a fish body length estimation method and system based on a global guidance semantic segmentation network, relates to the technical field of computer vision and artificial intelligence, and aims to solve the problems that in a scene that a fish body in a monocular image and complex background interference coexist, and in a multi-fish-species mixed scene, the model segmentation generalization ability in the prior art is insufficient, and the fish body length estimation accuracy is poor. And high-precision and robust mask segmentation is difficult to realize. The method comprises the following steps: acquiring a fish body monocular image, and performing distortion correction on the fish body monocular image; inputting the corrected fish body monocular image into a trained global guide semantic segmentation network for semantic segmentation to obtain a semantic segmentation mask graph of the fish body and the calibration plate, and constructing a mapping relationship between the physical size and the pixel size of the calibration plate; and performing ellipse fitting on the semantic segmentation mask graph of the fish body, calculating the pixel length of the fish body, and converting the pixel length of the fish body into the actual length of the fish body. The method solves the problems in the prior art, provides the generalization ability of the network model, and achieves the precise estimation of the length of the fish body.

The present invention relates to an additive that comprises compounds of plant and mineral extracts, useful in recirculating aquaculture system that will significantly improve efficiency of the biofilter used in said systems, thus reducing the levels of toxic chemicals such as ammonium and nitrite. Furthermore, this additive will also reduce oxygen consumption and provide better overall water quality conditions. This implies an improvement in the efficiency of these systems, thus achieving farming of different fish species with lower mortality, better feed conversion, and growth rate.

The invention provides a method for correcting and counting the natural breeding scale of fishes laying drifting eggs. The method comprises the following steps: collecting basic data of a target river section; collecting roes and fries of fishes laying drifting roes day by day at the lower boundary of the target river section, obtaining the number of early resources every day, sampling time, and collecting flow velocity and flow data near a net opening; through indoor culture and biological identification, the egg and fry development time of the fishes laying the drifting eggs is determined; based on the collected basic data, a target river section non-constant flow mathematical model is constructed, and model parameters are verified; according to the non-constant flow mathematical model, determining the specific section position of each fish laying the drifting eggs during egg laying; and calculating the flow corresponding to the section where the fishes laying the drifting eggs lay eggs and the flow velocity of the fishes drifting to the monitoring section from the downstream, and counting and calculating the overall spawning scale condition of the target river section during the monitoring period by adopting a spawning scale statistical method of the fishes laying the drifting eggs. And the natural breeding scale condition of the fishes in the target river section can be reflected more truly.

The invention discloses a breeding system based on a fan single pile foundation and a control method of the breeding system. The breeding system comprises a breeding cabin bottom, a liquid level monitoring device, a water circulation device, an aeration oxygenation device, a ventilation device, an automatic feeding device, a water quality monitoring device, a fry throwing and fish collecting device, an underwater monitoring device, a power supply device and a control system. According to the method, inexhaustible seawater resources are utilized, an open circulating water mode is adopted, the temperature and salinity of a water body do not need to be adjusted, the culture environment in the most original state is simulated, the original life habits of fingerlings do not need to be changed, and the quality of the culture variety is improved; meanwhile, a fan single-pile foundation is combined, the original useless internal space and electric energy produced by a wind field are reasonably utilized, wind power cultivation is fused, the method is green and low-carbon, a water body foundation does not need to be additionally built, the comprehensive cost is reduced, and the overall yield of equipment is increased.

The invention discloses a lightweight multi-fingerling and fry counting device based on computer vision and a use method thereof, and belongs to the technical field of machine vision and machinery, and the lightweight multi-fingerling and fry counting device comprises a water flow conveying device and a counting and processing device. Wherein the water flow conveying equipment is composed of a fish inlet funnel, two different water conveying pipelines, a fish fry slideway and a fish outlet, the fish inlet is funnel-shaped, water flow conveying is adopted, the inclination angle of the water flow conveying equipment can ensure that fish fries are not clamped in the fish inlet, meanwhile, the water flow is ensured to wash the fish fries into the slideway, and the water conveying pipelines are all plastic pipelines. The fish inlet funnel and the fry slideway are made of plastic materials with frosted surfaces, and are more resistant to seawater corrosion compared with metal materials. The counting and processing device is composed of a camera at the top of the device, a displayer and a computer host at the bottom, and the compatibility and real-time computing capacity of different algorithms are effectively improved. According to the invention, counting work can be carried out on different types of fish, a large amount of plastic and lightweight aluminum metal are used, and the size and the weight of equipment are obviously reduced.

The invention relates to a method for preparing all-female fingerlings of land-sealed Malus salicina by utilizing hydrostatic pressure, and belongs to the technical field of aquaculture. The method for preparing the all-female fingerling of the land-sealed Malus salicina by utilizing the hydrostatic pressure comprises the following steps: taking eggs from female land-sealed Malus salicina in a mature period, soaking the obtained eggs in an isotonic solution for 5-10 minutes, performing hydrostatic pressure treatment in the isotonic solution, taking out the obtained parthenogenetic development eggs, and putting the eggs into a hatching nest for hatching. The method is short in time consumption, can meet the requirement for one-time mass production of all-female fry of the Malus salicina, has the advantages of being low in production cost, easy to operate, time-saving, labor-saving and efficient, and can achieve the effect that the all-female rate of the hatched parthenogenetic fry is 100%.

The invention belongs to the technical field of robots, and relates to a bionic robotic fish coupled with fish luring factors, which is characterized in that a fish head, a fish body, a tail fin and a dorsal fin form a robotic fish shell; the robot further comprises two discharge electrodes, a communication module, two obstacle avoidance sensors, a water pump, two RGB lamps, three joint steering engines, a propeller thruster, two loudspeakers, a balance weight adjusting bin, a control circuit board, a battery, a water bin and a high-pressure air bin. Three fish luring factors including sound, light and electricity are coupled to the robotic fish, the combination mode of the factors is reasonably regulated, controlled and optimized, the fishes are guided to gather, the fishway entrance is found, the recognition ability of the fishes to fish passing facilities is enhanced, and the use efficiency of the fishway is improved. The method helps the migration of the rare fish fingerlings, protects the reproduction of the rare fishes, maintains the stability of fish populations, is beneficial to the protection and restoration of the ecological environment of the fishes, and walks the way of ecological priority and green development on the basis of the development of water conservancy projects.

Disclosed are a probe method-based kit and method for tracking a behavior trajectory of Acipenser dabryanus Dumeril released upstream of the Yangtze River. By analyzing mitochondrial DNA sequences of Acipenser dabryanus Dumeril and common fish species in the Yangtze River basin, a pair of specific primers ND5-F4 / ND5-R4 capable of specifically amplifying mitochondrial DNAs of Acipenser dabryanus Dumeril and a Taqman fluorescent probe ND5 Probe 4 are designed and screened. A kit composed of the specific primers, the Taqman fluorescent probe, and a ddPCR master mix is used to carry out ddPCR testing on environmental DNA samples collected before and after release, so that a behavior trajectory of the released Acipenser dabryanus Dumeril can be tracked and monitored, achieving "non-contact" tracking of the behavior trajectory of the released Acipenser dabryanus Dumeril, avoiding damage to fish bodies in the tracking process, leading to reduced tracking costs and improved accuracy of overall tracking.

The invention discloses a fish repelling method and system based on multi-frequency sound wave cooperation and dynamic environment perception. The method comprises the steps that water area environment parameters are acquired, and the types and the density of fishes in the water area are recognized through sonar, water velocity and water quality parameters; matching an auditory sensitive frequency band set according to the fish species, and constructing a multi-frequency-band sound wave emission parameter set in combination with the water area environment parameters; according to the sound wave emission parameter set, the transducer array is controlled to emit a multi-band composite sound wave signal, and a sound field with a coverage angle of at least 180 degrees is formed; and monitoring the driving effect in real time, and dynamically adjusting the multi-band sound wave emission parameter set according to the density change of the fish school. According to the method, the fish repelling efficiency can be remarkably improved, the influence on non-target species is reduced, and an innovative solution is provided for ecological protection of water conservancy projects.

The invention belongs to the field of fish germplasm improvement and innovation, and discloses an application of river perch sperms in inducing gynogenesis of largemouth bass, which comprises the following steps: obtaining mature largemouth bass ova and river perch sperms through artificial spawning induction; the method comprises the following steps: collecting river perch semen, diluting, oscillating, irradiating and inactivating with an ultraviolet lamp, mixing with mature largemouth bass ova, inseminating, and hatching fertilized ova to obtain gynogenesis largemouth bass. According to the method, the sperms of the river perches are used for stimulating gynogenesis of the micropterus salmoides for the first time, the gynogenesis micropterus salmoides is successfully obtained, and the heterologous sperms in the far geographic space are used as the stimulation source of the artificial gynogenesis ovum for the first time. The method not only provides an innovative germplasm resource basis in the aspect of improving germplasm of the micropterus salmoides, but also has important significance in the research aspects of fish genetic breeding and'micro-hybridization among fish species in far geographic space '.

The invention discloses a multi-species mutualism-based mussel and fish polyculture ecological regulation and control method, and relates to the technical field of ecological culture, and the method comprises the following steps: S1, facility preparation; s2, putting species; s3, breeding regulation and control; s4, feeding control; and S5, performing final-period management. Through strict screening of the culture pond, thorough disinfection and scientific cultivation of water quality, an excellent starting end is created for culture, the disease risk is reduced, beneficial microbial communities enhance the self-cleaning capacity of a water body, the feeding quantity and proportion are accurately calculated, the ecological capacity is fully utilized, the fingerlings are reasonably matched, stable operation of an ecological system is guaranteed, and the ecological system is more intelligent and reliable through real-time monitoring and intelligent prediction. The feeding amount and the water changing frequency are adjusted in time, the water quality is effectively maintained, the breeding loss caused by water quality deterioration is reduced, grass carp feeding is adjusted according to the aquatic plant coverage rate, reasonable growth of aquatic plants is maintained, ecological balance is promoted, the win-win situation of ecological benefits and economic benefits is achieved, and an effective mode is provided for sustainable aquaculture.

The invention relates to the technical field of biology, in particular to a primer combination, application of the primer combination and a method for qualitatively and quantitatively detecting target fish based on eDNA. The invention provides a primer combination, a kit and application of the primer combination to detection of fish species and / or detection of fish quantity. A target species is taken as a detection object, water sample environment DNA is extracted, a specific primer is used for PCR amplification, and an amplification product is subjected to sequencing analysis, so that whether a water body contains the target species or not is obtained, plasmids corresponding to target fragments of the target species are constructed on the basis, and a standard curve of the copy number and the cycle number of the target species is successfully drawn; and quantitatively analyzing the target species fish in an unknown water area.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an interference-free zebra fish micro-fluidic chip and an application method thereof. The micro-fluidic chip comprises a channel assembly, a limiting opening and a containing channel; the channel assembly is connected with one side of the limiting opening; the accommodating channel is connected with the other side of the limiting opening; wherein the channel assembly is configured to be capable of carrying out self-adaptive adjustment on the height and the width according to the size of the zebra fish. According to the non-invasive monitoring method realized based on the non-interference zebra fish micro-fluidic chip, stress reaction to the zebra fish in the process of fixing the zebra fish through manual operation is avoided, the use of anesthetic is avoided, the physiological state of the zebra fish is not influenced, the accuracy and the objectivity of experimental data are ensured, and the monitoring accuracy is improved. The micro-fluidic chip can be used for adapting to juvenile zebrafish with different development time, or can be used for observation and shooting recording of other fish fingerlings, and the universality of the micro-fluidic chip is improved.

This image analyzes echo data of schools of fish detected and collected by a fish finder or other device installed at a specific location, and displays information on the fish species detected by the software. As shown in the reference image diagram explaining each part, it consists of a date display area, a fish species information display area, and a table area. The fish species information display area by installation location, surrounded by a pink line in the reference image diagram explaining each part, is displayed for each installation location of the fish finder or other device. The graph area displays a bar graph showing the number of times the detected fish species was detected, the catch record display area displays the actual catch amount by fish species entered by the user, and the catch record editing area displays an image for the user to enter the actual catch amount by fish species. The table area displays a table showing the fish species detected by time period at a specific installation location and the number of times the fish species was detected.

The invention discloses a fishway hydraulic power and fishway structure design method and related equipment, and the method comprises the steps: obtaining swimming capability parameters of each target fish species in a target water area, the swimming capability parameters comprising an induction flow rate, a critical swimming speed and an outbreak swimming speed; selecting the target fingerling with the minimum critical swimming speed as a limited fingerling; according to the critical swimming speed corresponding to the limited fingerling, calculating a first energy consumption rate of swimming of the limited fingerling at the first hydraulic flow speed; according to the first energy consumption rate, hydraulic function partitions and space sequence layout of the fishway are determined; and determining fishway hydraulic parameters according to the induction flow velocity of each target fingerling and the critical swimming speed and the outbreak swimming speed of the limited fingerling. According to the fishway, inclusive passing of the fishway to multiple species can be achieved, the structural design of the fishway can adapt to the natural behavior rhythm of fishes, the overall energy consumption of dam passing of fish schools is remarkably reduced, then the ecological efficiency of the fishway is improved, and the fishway can be widely applied to the technical field of fishway design.

The invention discloses a method for increasing the survival rate of bighead carp saline-alkali water increased culture fingerlings, and relates to the technical field of fish culture. The method aims at solving the problems that in the medium-high saline-alkali water culture and proliferation process of bighead carps in northeast China, when fingerlings cultured in a fresh water pond come out of the pond and are directly put into medium-high saline-alkali water in spring and autumn, the fish bodies have severe stress reactions, and when the stress reactions exceed the bearing range of the fish bodies, individual death often occurs, and the survival rate of the bighead carps is high. The survival rate of the thrown fingerlings is low; and the economic benefit of culture and proliferation is poor. According to the method, the target saline-alkali lake, the foul marsh and other water bodies are used for preparing the low-alkali pond water body with the maximum alkalinity value of 1 / 3-1 / 2 concentration of bighead carp fingerling saline-alkali water proliferation and culture, through adaptive domestication of fish bodies, the stress resistance of the fish bodies can be enhanced, and the stress reaction of fingerling cultured in a fresh water pond directly put into the medium-high saline-alkali water body is relieved. The method for improving the survival rate of the bighead carp saline-alkali water increased culture fingerling can be obtained.

The invention discloses an X-chromosome specific molecular marker of micropterus salmoides and application of the X-chromosome specific molecular marker. On the basis of a whole genome sequencing technology, the X chromosome specific molecular marker of micropterus salmoides is obtained. Aiming at the molecular marker, the invention also provides a detection primer pair and a detection method of the molecular marker, and by detecting the molecular marker, rapid and accurate screening of YY supermale fish individuals can be realized to obtain a YY supermale fish population. The obtained YY super-male fish group and a proper female fish group are jointly fed and bred, and then the micropterus salmoides all-male fingerling can be obtained. If the YY super-male fish group and the YY pseudo-female fish group are jointly bred and bred, largemouth bass offspring which is all YY super-male fish can be obtained. Compared with a traditional all-male breeding method of the largemouth black bass, the all-male breeding method of the largemouth black bass is beneficial to simplifying all-male breeding steps of the largemouth black bass, shortening breeding time, and beneficial to cultivation of all-male largemouth black bass fingerlings and popularization of single-male breeding of the largemouth black bass.

A comprehensive system for hatching drifting fish eggs includes a culture tank, a flow control system, a roundabout real-time monitoring system, a stepped egg transfer system, an egg release system, an egg collection system, a tailwater collection platform, and a water storage tank. This system eliminates the influence of previous single-factor conditions on hatching, achieving the adjustability of influencing factors under different hatching environments, real-time tracking of the entire egg hatching process, and simulation of natural water flow conditions. During the hatching process, the system replaces the cumbersome operation of multiple manual egg releases in previous patents with a single, fully automated egg release process, thereby improving the egg hatching rate. Furthermore, to identify the drifting path patterns of the eggs during hatching, side-view and top-view egg drift monitoring systems and a hydraulic characteristics testing system are provided. The hatching drift distance of the eggs, as calculated by the monitoring system, can provide a reference for the fish species richness of the river's main stream and tributaries.