7 results about "Acipenser dabryanus" patented technology

The invention provides a primer probe combination and a kit for detecting eDNA of acipenser Yangtze River and application of the primer probe combination and the kit, and belongs to the technical field of molecular biology and molecular ecology. The invention designs two groups of primer probe combinations for detecting eDNA of acipenser Yangtze River, and the nucleotide sequences of the primer probe combinations are respectively shown as SEQ ID No.1-6. According to the method, eDNA in a water sample to be detected is extracted, ddPCR amplification is carried out by utilizing the primer probe combination, if positive amplification can occur, it is judged that the water sample contains the eDNA of the Yangtze River sturgeons, and then tracking of behavior tracks of Yangtze River sturgeon schools is achieved. The primer probe combination disclosed by the invention can be used for carrying out specific detection on target DNA fragments of the acipenser pratus in a culture water body and a field water body, the sensitivity is relatively high and is 0.07 copies / mu L, and the primer probe combination can be widely applied to the fields of eDNA detection of the acipenser pratus and resource protection of rare fishes.

The present invention provides a method for artificial propagation of Acipenser dabryanus, which includes selecting broodstock, pairing broodstock, culturing broodstock, artificial induced spawning, artificial insemination, hatching of fertilized eggs and culturing of juvenile fish. The present invention stimulates the gonadal development of broodstock by precisely controlling the water temperature during broodstock cultivation, selects broodstock with relatively distant genetic relationships for pairing through the identification of the genetic relationships of broodstock to overcome the problem of population degradation caused by inbreeding, and uses artificial insemination, hatching of fertilized eggs and juvenile fish cultivation techniques to solve the problem of difficult artificial propagation of existing Acipenser dabryanus, so as to stably and continuously supply high-quality Acipenser dabryanus seeds and meet the perennial effective demand for artificial enhancement and release of Acipenser dabryanus.

The application provides a primer composition, a kit for detecting MNP marker sites of Yangtze sturgeon and application thereof. The primer composition comprises 100 pairs of primer pairs, and the nucleotide sequences of the 100 pairs of primer pairs are shown in SEQ ID NO:1-SEQ ID NO:200. By using the primer composition, not only the Yangtze sturgeon species or individual can be identified, but also the Yangtze sturgeon species or genetic diversity in the water area can be identified based on the water environment DNA, and the primer composition has the advantages of high throughput, strong distinguishability, good accuracy and high accuracy, thereby providing technical support for scientific research, fishery monitoring and ecological system investigation of the Yangtze sturgeon.

Disclosed are a probe method-based kit and method for tracking a behavior trajectory of Acipenser dabryanus Dumeril released upstream of the Yangtze River. By analyzing mitochondrial DNA sequences of Acipenser dabryanus Dumeril and common fish species in the Yangtze River basin, a pair of specific primers ND5-F4 / ND5-R4 capable of specifically amplifying mitochondrial DNAs of Acipenser dabryanus Dumeril and a Taqman fluorescent probe ND5 Probe 4 are designed and screened. A kit composed of the specific primers, the Taqman fluorescent probe, and a ddPCR master mix is used to carry out ddPCR testing on environmental DNA samples collected before and after release, so that a behavior trajectory of the released Acipenser dabryanus Dumeril can be tracked and monitored, achieving "non-contact" tracking of the behavior trajectory of the released Acipenser dabryanus Dumeril, avoiding damage to fish bodies in the tracking process, leading to reduced tracking costs and improved accuracy of overall tracking.

The invention discloses an SNP molecular marker for identifying the brood amount of female sturgeons and application of the SNP molecular marker, and belongs to the field of sturgeon genetic breeding and sturgeon molecular marker-assisted breeding. The SNP molecular marker for identifying the brood amount of the female sturgeon is located at the 117bp position of a GnA gene nucleotide sequence fragment; the polymorphic form of the SNP site is C / A, and the genotype of the SNP site comprises CC, CA or AA. Wherein the female individuals with the genotype of AA belong to low-brood-amount individuals, and the female individuals with the genotypes of CC and CA belong to high-brood-amount individuals. The molecular marker provided by the invention can be used for early-stage artificial screening of female sturgeon brood quantity characters, screening can be carried out in various female sturgeon groups such as acipenser schrenckii, Huso dauricus, acipenser sibiricum and hybrid sturgeons, and the artificial breeding process of sturgeon varieties with high brood quantity can be remarkably accelerated.

The invention discloses a primer for detecting a Yangtze River acipenser body weight character related SNP molecular marker. The primer is characterized by comprising an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2. The method can be used for breeding Yangtze River sturgeon juveniles with body weight character advantages and selecting parents. The invention firstly provides an SNP site related to the body weight character of acipenser Yangtze River. The SNP site exists in a low-weight population in sexual mature acipenser dungryanus individuals, and the technology can provide technical support for protection of acipenser dungryanus.

A SNP molecular marker for identifying the high and low egg production of female sturgeons and its application belong to the field of sturgeon genetic breeding and sturgeon molecular marker assisted breeding. The specific scheme is: the SNP molecular marker for identifying the high and low egg production of female sturgeons is located at the 333rd bp of the 17β-hsd2 gene nucleotide fragment, its polymorphic form is C / G, and the genotype of its base mutation site includes CC, CG or GG. Female individuals with a genotype of GG belong to low egg production individuals, and female individuals with a genotype of CC or CG belong to high egg production individuals. The molecular marker provided by the present invention can be used for early artificial breeding of the egg production trait of female sturgeons, and can be widely used in a variety of sturgeon female groups such as Acipenser schrenckii, Acipenser dabryanus, Acipenser Siberianus and hybrid sturgeon, thereby significantly accelerating the breeding process of high-egg-producing sturgeon varieties.