4 results about "Acipenser dabryanus" patented technology

The application provides a primer composition, a kit for detecting MNP marker sites of Yangtze sturgeon and application thereof. The primer composition comprises 100 pairs of primer pairs, and the nucleotide sequences of the 100 pairs of primer pairs are shown in SEQ ID NO:1-SEQ ID NO:200. By using the primer composition, not only the Yangtze sturgeon species or individual can be identified, but also the Yangtze sturgeon species or genetic diversity in the water area can be identified based on the water environment DNA, and the primer composition has the advantages of high throughput, strong distinguishability, good accuracy and high accuracy, thereby providing technical support for scientific research, fishery monitoring and ecological system investigation of the Yangtze sturgeon.

Disclosed are a probe method-based kit and method for tracking a behavior trajectory of Acipenser dabryanus Dumeril released upstream of the Yangtze River. By analyzing mitochondrial DNA sequences of Acipenser dabryanus Dumeril and common fish species in the Yangtze River basin, a pair of specific primers ND5-F4 / ND5-R4 capable of specifically amplifying mitochondrial DNAs of Acipenser dabryanus Dumeril and a Taqman fluorescent probe ND5 Probe 4 are designed and screened. A kit composed of the specific primers, the Taqman fluorescent probe, and a ddPCR master mix is used to carry out ddPCR testing on environmental DNA samples collected before and after release, so that a behavior trajectory of the released Acipenser dabryanus Dumeril can be tracked and monitored, achieving "non-contact" tracking of the behavior trajectory of the released Acipenser dabryanus Dumeril, avoiding damage to fish bodies in the tracking process, leading to reduced tracking costs and improved accuracy of overall tracking.

The invention discloses an SNP molecular marker for identifying the brood amount of female sturgeons and application of the SNP molecular marker, and belongs to the field of sturgeon genetic breeding and sturgeon molecular marker-assisted breeding. The SNP molecular marker for identifying the brood amount of the female sturgeon is located at the 117bp position of a GnA gene nucleotide sequence fragment; the polymorphic form of the SNP site is C / A, and the genotype of the SNP site comprises CC, CA or AA. Wherein the female individuals with the genotype of AA belong to low-brood-amount individuals, and the female individuals with the genotypes of CC and CA belong to high-brood-amount individuals. The molecular marker provided by the invention can be used for early-stage artificial screening of female sturgeon brood quantity characters, screening can be carried out in various female sturgeon groups such as acipenser schrenckii, Huso dauricus, acipenser sibiricum and hybrid sturgeons, and the artificial breeding process of sturgeon varieties with high brood quantity can be remarkably accelerated.

The invention discloses a primer for detecting a Yangtze River acipenser body weight character related SNP molecular marker. The primer is characterized by comprising an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO: 2. The method can be used for breeding Yangtze River sturgeon juveniles with body weight character advantages and selecting parents. The invention firstly provides an SNP site related to the body weight character of acipenser Yangtze River. The SNP site exists in a low-weight population in sexual mature acipenser dungryanus individuals, and the technology can provide technical support for protection of acipenser dungryanus.