5 results about "Molecular ecology" patented technology

The invention provides a primer probe combination and a kit for detecting eDNA of acipenser Yangtze River and application of the primer probe combination and the kit, and belongs to the technical field of molecular biology and molecular ecology. The invention designs two groups of primer probe combinations for detecting eDNA of acipenser Yangtze River, and the nucleotide sequences of the primer probe combinations are respectively shown as SEQ ID No.1-6. According to the method, eDNA in a water sample to be detected is extracted, ddPCR amplification is carried out by utilizing the primer probe combination, if positive amplification can occur, it is judged that the water sample contains the eDNA of the Yangtze River sturgeons, and then tracking of behavior tracks of Yangtze River sturgeon schools is achieved. The primer probe combination disclosed by the invention can be used for carrying out specific detection on target DNA fragments of the acipenser pratus in a culture water body and a field water body, the sensitivity is relatively high and is 0.07 copies / mu L, and the primer probe combination can be widely applied to the fields of eDNA detection of the acipenser pratus and resource protection of rare fishes.

The invention discloses a primer and a method for quantitatively monitoring the biomass of sargassum hemiphyllum based on an environmental DNA technology, and belongs to the technical field of molecular ecology. The primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are specifically targeted to the sargassum hemiphyllum mitochondria COX1 gene, and the sequence is shown as SEQ ID NO.1-3. The kit comprises the primer probe group, a qPCR (quantitative polymerase chain reaction) premixed solution, nuclease-free water and a sargassum hemiphyllum plasmid positive control. The method comprises the following steps: collecting a water sample, enriching eDNA, performing qPCR detection by using the primer probe group after extraction and purification, and realizing qualitative detection and quantitative evaluation of sargassum hemiphyllum through a Cq value or a standard curve. The method disclosed by the invention has the advantages of high sensitivity, strong specificity, no damage to the environment and target organisms, capability of realizing large-scale rapid general survey and the like, and is suitable for early warning of gulfweed blooms, investigation of population distribution and evaluation of ecological influence.

The present application relates to the technical field of molecular biology and molecular ecology, and discloses a primer probe combination for detecting eDNA of Pseudobrama lantschichi, a kit and application thereof.The present application provides a primer probe combination for detecting eDNA of Pseudobrama lantschichi, and the nucleotide sequences are shown in SEQ ID No.1-3.The primer probe combination can specifically detect low-concentration eDNA of Pseudobrama lantschichi in water samples, and can detect the concentration of eDNA of Pseudobrama lantschichi in water samples by microdroplet digital PCR (ddPCR), and the detection sensitivity is 0.07 copies / μL, which is high.Further, the eDNA of Pseudobrama lantschichi can be non-invasively, quickly and accurately tracked, and the method has great ecological significance.

The present invention relates to the field of molecular ecology, and in particular to a sex identification marker, an identification method and an application of a black carp. The black carp sex identification marker is selected from at least one of Mar26, Mar28, Mar37 and Mar38; wherein Mar26 is composed of an upstream primer shown in SEQ ID NO: 51 and a downstream primer shown in SEQ ID NO: 52; Mar28 is composed of an upstream primer shown in SEQ ID NO: 55 and a downstream primer shown in SEQ ID NO: 56; Mar37 is composed of an upstream primer shown in SEQ ID NO: 73 and a downstream primer shown in SEQ ID NO: 74; Mar38 is composed of an upstream primer shown in SEQ ID NO: 75 and a downstream primer shown in SEQ ID NO: 76. Furthermore, the sex identification marker is Mar28, and the provided sex identification marker can accurately and efficiently identify the sex of the black carp without being affected by the developmental period. The method for genetic sex identification of black carp established by the present invention can be helpful for application in the monosexual breeding of black carp.