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3 results about "Molecular ecology" patented technology
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Molecular ecology is a field of evolutionary biology that is concerned with applying molecular population genetics, molecular phylogenetics, and more recently genomics to traditional ecological questions (e.g., species diagnosis, conservation and assessment of biodiversity, species-area relationships, and many questions in behavioral ecology). It is virtually synonymous with the field of "Ecological Genetics" as pioneered by Theodosius Dobzhansky, E. B. Ford, Godfrey M. Hewitt and others. These fields are united in their attempt to study genetic-based questions "out in the field" as opposed to the laboratory. Molecular ecology is related to the field of Conservation genetics.
Primer and method for quantitatively monitoring biomass of sargassum hemiphyllum based on environmental DNA (Deoxyribose Nucleic Acid) technology
ActiveCN121737340AMicrobiological testing/measurementDNA/RNA fragmentationSargassum hemiphyllumDeoxyribose
The invention discloses a primer and a method for quantitatively monitoring the biomass of sargassum hemiphyllum based on an environmental DNA technology, and belongs to the technical field of molecular ecology. The primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are specifically targeted to the sargassum hemiphyllum mitochondria COX1 gene, and the sequence is shown as SEQ ID NO.1-3. The kit comprises the primer probe group, a qPCR (quantitative polymerase chain reaction) premixed solution, nuclease-free water and a sargassum hemiphyllum plasmid positive control. The method comprises the following steps: collecting a water sample, enriching eDNA, performing qPCR detection by using the primer probe group after extraction and purification, and realizing qualitative detection and quantitative evaluation of sargassum hemiphyllum through a Cq value or a standard curve. The method disclosed by the invention has the advantages of high sensitivity, strong specificity, no damage to the environment and target organisms, capability of realizing large-scale rapid general survey and the like, and is suitable for early warning of gulfweed blooms, investigation of population distribution and evaluation of ecological influence.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
A primer probe combination, kit and application thereof for detecting eDNA of rutilus rutilus
The present application relates to the technical field of molecular biology and molecular ecology, and discloses a primer probe combination for detecting eDNA of Pseudobrama lantschichi, a kit and application thereof.The present application provides a primer probe combination for detecting eDNA of Pseudobrama lantschichi, and the nucleotide sequences are shown in SEQ ID No.1-3.The primer probe combination can specifically detect low-concentration eDNA of Pseudobrama lantschichi in water samples, and can detect the concentration of eDNA of Pseudobrama lantschichi in water samples by microdroplet digital PCR (ddPCR), and the detection sensitivity is 0.07 copies / μL, which is high.Further, the eDNA of Pseudobrama lantschichi can be non-invasively, quickly and accurately tracked, and the method has great ecological significance.
Owner:INST OF AQUATIC LIFE ACAD SINICA +1
A real-time monitoring system for molecular ecology of harmful red tide algae in sea based on buoy platform
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI