33 results about "Molecular ecology" patented technology

The invention relates to the field of microorganism oil-gas resource exploration and discloses a method for oil-gas resource exploration indicating by utilizing molecular ecology. The method includes utilizing a molecular ecology studying means to calibrate fingerprint spectra of microorganism species in topsoil or sediment; comparing the fingerprint spectra with references of point locations where underlaying oil-gas reservoirs exist in a known manner to complete dividing of potential abnormality areas and background areas of oil-gas resources. By the method, abnormality dividing of potential oil-gas-resource-containing areas can be realized more accurately, so that exploration success rate is increased.

The invention discloses a gene target therapy scheme recommendation system. The system comprises a case data cluster module, a similar case acquisition module, a neural network training module and a therapy scheme recommendation module, wherein the case data cluster module is used for classifying multiple case data according to molecular ecology network analysis to obtain multiple case sets, wherein case data comprise gene data and case feature data; the similar case acquisition module is used for screening cases according to similarity between the current case and each case set to obtain similar case sets; the neural network training module is used for neural network training according to the similar case sets and corresponding therapy schemes to obtain a scheme recommendation model; thetherapy scheme recommendation module is used for calculating the current case according to the scheme recommendation model to obtain a target therapy scheme. Useless data are reduced by screening andthe model accuracy is improved. The invention further discloses another gene target therapy scheme recommendation system with the benefits.

The invention discloses a bio-augmentation technology for fermentation process of solid-state brewing vinegar, belonging to the technical field of biological Engineering. The operation procedures of the method comprise: (1) determining functional microorganisms for brewing vinegar: analyzing the structure of the microflora in vinegar fermented grains with molecular ecology technology such as PCR-DGGE, determining the functional microorganisms in the process of brewing vinegar by combining the key physicochemical indexes related to the quality and functionality of vinegar; (2) pure culture of the functional microorganisms for brewing vinegar: using microorganism pure culture technique to get the functional microorganisms for brewing vinegar from the vinegar fermented grains; and (3) bio-augmentation in the vinegar fermentation process: selecting the functional microorganisms or a combination thereof, and carrying out bio-augmentation in the solid-state fermentation stage of vinegar. The method has the advantages of shortening brewing cycle of vinegar, improving and controlling the flavor, quality and functionality of vinegar products, improving the utilization rate of raw material and the like.

The invention discloses a method for the extraction and the purification of microbial total DNA of a compost and a buffer solution thereof, the method comprises the following steps of: adding a dehumification solution in an amount of 8-15ml/g of the compost into a sample of the compost in which the DNA is to be extracted, for dehumification; then adding a DNA extraction buffer solution in an amount of 1.0-1.5ml/g of the compost for the crude extraction of the microbial total DNA; and finally, purifying the crudely-extracted DNA solution with a kit so as to obtain the microbial total DNA of the compost. The inventive method can reduce the influence of humic acids and effectively improve the yield and the purity of the DNA extracted from the compost, thus better facilitating compost microbial molecular ecology and researches of other functional genes.

The invention relates to soil microbial molecular ecology and particularly relates to a method for distinguishing a soil N2O emission source. The method comprises the following steps of: after treating soil to be analyzed, determining the isotope delta 15N value of N2O emitted by the soil and the copy number of related microbial genes in the process that the soil produces the N2O, and judging that functional microbial groups with correlation test p <0.05 between gene abundance and delta 15N-N2O abundance value are microbial groups which play an important role in soil N2O emission. The method is applicable to the distinguishing of the microbial groups with N2O emission function in certain soil.

The invention belongs to the field of microbial molecular ecology detection, and particularly relates to a kit and a method for rapidly detecting archaea. According to the kit and the method for rapidly detecting archaea, the problems existing in abundance, diversity, composition and structure of archaea communities under different environments in real-time fluorescent quantitative PCR and high-throughput sequencing at present are solved, and the kit for quickly detecting archaea comprises a primer group, a DNA fragment, a PCR buffer solution and an STE buffer solution; in addition, the DGGE technology is used for rapidly detecting the archaea, the experiment cost is low, operation is easy and convenient, and meanwhile the method has the advantages of being high in specificity, easy to popularize in a laboratory, independent of large experiment equipment and the like; and the method is an efficient, rapid, economical and convenient detecting method.

The invention relates to a method for acquiring high-quality soil microbial genome DNA through re-extraction by pretreating a soil sample. In the invention, a solution of calcium carbonate and the like is used for pretreating a soil sample to be subjected to genome DNA extraction, the extracted DNA has clear appearance color, and the DNA with OD260/OD280 of 1.81 has high purity. The extracted DNA can be used for PCR (Polymerase Chain Reaction) amplification directly, and a DGGE (Denaturing Gradient Gel Electrophoresis) map indicates that an amplified product has high concentration and sharp banding patterns. Shannon index indicates that the amplified product has great diversity and can effectively reflect the microorganism diversity of a soil microbial community. The pretreatment technology provided by the invention is simple and feasible, has the advantages of low cost, obvious effect on removing humic acid substances and high quality of extracted genome DNA, and is suitable for the extensive research field of molecular ecology.

The invention provides a method for detecting fish species diversity by using environmental DNA, and relates to the technical field of molecular ecology. The method for detecting fish species diversity by using the environmental DNA comprises the following steps: collecting a water sample of a place to be detected, filtering, extracting eDNA to obtain eDNA of the water sample to be detected, carrying out PCR amplification by using a primer group to obtain an amplification product, carrying out high-throughput sequencing on the amplification product to obtain sequence fragments, splicing the sequence fragments, and carrying out OTU cluster analysis on the species diversity. The primer group comprises an upstream primer and a downstream primer, the nucleotide sequence of the forward primer is as shown in SEQID.NO 1, and the nucleotide sequence of the reverse primer is as shown in SEQID.NO 2. The method has the advantages that the method can be used for detecting the diversity of fish species, especially the diversity of lake aquatic organisms, the detection result is accurate, and multiple species can be detected.

The invention belongs to the technical field of biology, and provides a tetraena mongolica microsatellite locus and a primer sequence thereof. The tetraena mongolica microsatellite locus has a sequence as shown in SEQ ID NO: 1-10. The tetraena mongolica microsatellite locus can be used in molecular ecological research, pedigree analysis, germplasm resources investigation, assistant breeding and individual recognition of tetraena mongolica, and provides basic materials to studying plant drought resisting mechanisms.

The invention relates to a primer for monitoring DNA (Deoxyribose Nucleic Acid) of a fish environment in the middle and upper reaches of the Yarlung Zangbo River and an application method thereof, and belongs to the field of molecular ecology. The primers comprise the following components of YZR-Cytb-F:TGRCTTGAARAACCACCGTTGT and YZR-Cytb-R: AARAAGAAWGADGCKCCRTTDGCRTG. The method comprises the following steps of (1) collecting a water sample and extracting eDNA; (2) slightly transforming the primer and then carrying out primary PCR (Polymerase Chain Reaction) on eDNA (Deoxyribose Nucleic Acid); (3) carrying out secondary PCR on the purified product of the primary PCR by using an Illumina library building primer; (4) performing high-throughput sequencing on the purified product of the second PCR; and (5) analyzing the fish species composition in the water sample according to the sequencing data. The primer has an identification effect on the species level of all fishes in the middle and upper reaches of the Yarlung Zangbo River, and the method can be used for accurately and completely monitoring the species diversity of the fishes in the middle and upper reaches of the Yarlung Zangbo River on the premise of not damaging the environment and fish populations.

The invention belongs to the technical field of soil microbial community diversity analysis and discloses optimal conditions and reagent dosage in a PCR-DGGE (Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis) method used for analyzing microbial community diversity of soil polluted by petroleum. The analysis method comprises the following steps of: 1, extracting enough DNA from 0.4g of soil samples; 2, carrying out PCR (Polymerase Chain Reaction) on 1 microliter of purified DNA; 3, performing a DGGE (denaturing gradient gel electrophoresis) experiment on 3-5 microliters of PCR products; 4, adding 9 microliter of TEMED (tetramethylethylenediamine) and 40 microliter of 10% APS (ammonium sulfate) into 18ml of modified acrylamide glue solution for preparing gel; 5, filling the gel, covering the top with a preservative film, standing still overnight to finally obtain excellent modified gel; 6, performing electrophoresis for 5 hours at the voltage of 120V and the electrophoresis temperature of 60 DEG C to obtain a good DGGE map; and 7, performing silver staining, wherein a stop solution is not used necessarily. The method is mainly used in the molecular ecology researches in the bioremediation process of soil polluted by petroleum, and also can provide references for molecular ecology researches of other environmental samples.

The invention relates to a DNA Marker specially used for coal geology environment bacteria species in molecular detection of denaturing gradient gel electrophoresis (DGGE) for coal geology environment microbial flora, and belongs to the field of microorganism molecular ecology. According to the DNA Marker for detecting the coal geology microorganism bacterium species, the DNA Marker consists of 11 DNA segments a to k, wherein the nucleotide sequence of DNA segment a is as shown in SEQ. ID. No.1; the nucleotide sequence of DNA segment b is as shown in SEQ. ID. No.2; the nucleotide sequence of DNA segment c is as shown in SEQ. ID. No.3; the nucleotide sequence of DNA segment d is as shown in SEQ. ID. No.4; the nucleotide sequence of DNA segment e is as shown in SEQ. ID. No.5; the nucleotide sequence of DNA segment f is as shown in SEQ. ID. No.6; the nucleotide sequence of DNA segment g is as shown in SEQ. ID. No.7; the nucleotide sequence of DNA segment h is as shown in SEQ. ID. No.8; the nucleotide sequence of DNA segment i is as shown in SEQ. ID. No.9; the nucleotide sequence of DNA segment j is as shown in SEQ. ID. No.10; and the nucleotide sequence of DNA segment k is as shown in SEQ. ID. No.11.

The invention belongs to the field of microbial molecular ecology detection, and particularly relates to a kit and a method for rapidly detecting coal geological environment bacteria. Aiming at the problem of limitation of environment bacteria detection in the prior art, the invention provides the kit for rapidly detecting the coal geological environment bacteria. The kit comprises a primer group and a DNA fragment for rapidly detecting the coal geological environment bacteria, a PCR buffer solution and an STE buffer solution. In addition, the geological environment bacteria are rapidly detected by using a DGGE technology, the result is reliable, the experiment speed is high, the specificity is good, the sensitivity is high, and a plurality of samples can be analyzed at the same time. The price of experimental consumables is low, and the experimental technology is easy to popularize and simple to operate. Compared with a general detection method, the detection method is efficient, rapid, economical and convenient.

The invention discloses an extracting method of cellulose degradation flora metagenome and provides an extracting method of cellulose degradation flora metagenome DNA. The extracting method comprises the following steps that cellulose degradation florae are sequentially subjected to thallus wall breaking, DNA organic solution extraction, DNA isoamylol sediment and DNA solution so that the metagenome DNA can be obtained. Experiments prove that a high-salt solution is adopted for carrying out eluting before extraction, lysozyme and mechanical homogenate are added to reinforce cell wall breaking, proteinase K is adopted for wall breaking, cell walls of microorganisms can be broken more easily, and DNA molecules can be released more easily. Meanwhile, the process repeatability is effectively ensured, and then integrity and purity of extracted metagenome DNA fragments and flora structure integrity are ensured. The extracting method provides an effective technology support for study and application of the natural cellulose utilizing florae and the microorganism molecular ecology study.