The invention relates to a streptomycete with dual functions of disease prevention and insecticide, its application, a cultivation method and a biocontrol agent, and belongs to the technical field of plant disease and insect pest control. In the present invention, an endophytic streptomyces MS001 with antibacterial activity is isolated; Botrytis cinerea, wheat scab, clivia stem rot, and nandina anthracnose all have strong antibacterial activity, and the antibacterial objects have a broad spectrum; and other plant pests. Therefore, Streptomyces MS001 can prevent and control some diseases and pests on apple trees, garden plants, vegetables, and food crops.