57 results about "Neurospora" patented technology

A microbial leaven used for preparing organic or microbial fertilizer from organic waste is prepared from at least two of nine microbes. Aspergillus, Sporotrichun, Mucoraceae, Penicillium sp, Ceotrichum sp., Neurospora, Bacillus, Saccharomyces and Streptomyces sp.

The invention relates to a feeding microecologic preparation which has high efficiency, is very low in cost, and is formed by performing three stages of probiotics fermentation to beer grains and rice bran meal, and is also called microbial forage additive. The feeding microecologic preparation is characterized in that after beer grains and rice bran meal are subjected to the first stage of fermentation preprocessing through neurospora, lignin, cellulose and the like are sufficiently degraded; part of rice bran meal and a small amount of soya bean meal are added to be subjected to the second stage of fermentation through bacillus and microzyme; and nutrient substances generated when waste is degraded by mould and nutrient substances generated when mould is dismembered are absorbed; and then part of rice bran meal and a small amount of soya bean meal are added to be finally subjected to the third stage of anaerobic fermentation through lactobacillus, bifidobacterium and the like in a one way membrane anaerobic bag, so that finished products are obtained. The feeding microecologic preparation has the advantages that solid material is not required to be subjected to high temperature sterilization, the finished products are not required to be subjected to drying process, the quality guarantee period is more than one year, the amount of additive reaches 5 to 30 percent, the amount of active probiotics is high, the amount of enzyme is abundant, the amount of bacterials is very little, the feeding effect is obvious, and the feeding microecologic preparation has obvious ecological and social benefits.

The invention discloses a method for improving production of a lignocellulosic enzyme line from filamentous fungi and an application of the filamentous fungi in fermentation of microbial bio-based chemicals, and belongs to the field of genetic engineering. According to the method, high-affinity glucose transporter of the filamentous fungi is transformed by virtue of such genetic engineering approaches as knocking-out, mutation, importing and the like, and an obtained engineering strain has the advantages. The filamentous fungi include, but not limited in, neurospora, myceliophthora, trichoderma, aspergillus, penicillin, fusarium and the like; genes of the transporter include NCU10021 (HGT-1) and a homologous gene thereof as well as NCU04963 (HGT-2) and a homologous gene thereof; any one or two genes are knocked out, or bases of any one or two genes are mutated, or expression of any one or two genes is decreased. The engineering strain, which is obtained by importing the transporter into a microbial strain in an exogenous mode or by changing endogenous expression of the transporter, can gain or enhance a glucose transporting and utilizing capacity, and subsequently, a capacity of producing such bio-based chemicals as organic acid, organic alcohol and the like through fermentation can be gained or improved.

An air-oxidative type hair dye composition containing a melanin precursor prepared by a process including (A) an oxidation step for converting, into the melanin precursor, a tyrosine or derivative thereof used as a starting substance with an enzyme or cell that is derived from a fungus selected from the group consisting of fungi belonging to the genera Aspergillus, Neurospora, Rhizomucor, Trichoderma, and Penicillium and that exhibits a catechol oxidase activity.

The present invention discloses a new-type feed and its preparation method. Said new-type feed is made up by using corn protein powder and wheat bran as raw material and using neurospora strain No.17 as fermentation zymophyte seed through a certain preparation process. Said preparation process includes the following steps: making slant cuture and solid fermentation, the ratio of corn protein powder and wheat bran is 3:2, the ratio of dried culture medium and water is 1:1.5 and the ratio of MgSO4 and culture medium is 0.01%, and making culture for 4 days at 25deg.C so as to obtain the invented new-type feed. The resolubility of protein in said new-type feed can be up to above 20%, and the antioxidant activity of said new-type feed can be up to above 200U / mL. Said new-type feed has high nutritional value.

The present invention has the technology scheme of adopting microbial zymogene preparation containing neurospora and saccharomycete. The fermentation process with the microbial zymogene preparation of the present invention to produce feed or alcohol is to add the microbial zymogene preparation components simultaneously or gradually and to process the obtained material for producing feed or alcohol. The present invention has the beneficial effect that the coupled and synergistic fermentation with the microbial zymogene preparation can eliminate the feedback suppression and saccharide or ethanol product suppression in the fermentation, raise the protein content, soften material, generate spirit fragrance, improve the taste of feed and raise production efficiency and product quality.

The invention relates to a composite efficient straw quickly-decomposing agent containing neurospora. The composite efficient straw quickly-decomposing agent is formed by compounding neurospora crassa, aspergillus oryzae, trichoderma viride, saccharomyces cerevisiae hansen, candida, bacillus subtilis, potassium bacteria, phosphate-solubilizing microorganism, azotobacter chroococcum and an accelerant and can be used for treating straw of wheat, corn, paddy and the like quickly. According to the composite efficient straw quickly-decomposing agent, efficient cellulose decomposition bacteria are introduced, corresponding enzyme activity accelerants are added, so that the activity of a cellulolytic enzyme is improved obviously, and the period of decomposing the straw is shortened within 15 days successfully. The composite efficient straw quickly-decomposing agent is used for the hazard-free treatment and recycling of agricultural wastes, the problems of the random stacking and uncontrolled incineration pollution of the straw are solved, and organic fertilizers and biological bacterial manures can also be produced, so that the using quantity of chemical fertilizers can be reduced, and the processes of farmland hardening and water and soil loss are retarded. The composite efficient straw quickly-decomposing agent can be sprayed on the surface of crop straw directly, so that the straw is covered, fermented and decomposed to be returned to fields.

The invention relates to Neurospora.crassa which is characterized by being named as Neurospora.crassa and is currently preserved by a preservation department designated by the State Intellectual Property Office. The preservation date is July 3rd, 2009; the preservation department is China General Microbiological Culture Collection Center; and the preservation number is CGMCC NO.3088. The invention has the advantages that: (1) a mature spore of Neurospora.crassa contains a large amount of natural beta-carotene; (2) the content of crude fiber can be greatly reduced, the quality of Neurospora.crassa is improved, and the utilization range of residues is expanded; (3) the residues of agricultural and sideline products through microbial fermentation are used, the raw material is easily acquired and is low in cost, and production equipment have low investment and great benefits; and (4) environmental pollution is reduced, the pressure of serious shortage of certain protein feeds can be alleviated, and a large amount of food is saved.

The present invention relates to a pretreatment method of a straw biological feed raw material and belongs to the technical field of biological feeds. Straw is used as a main raw material; firstly, alkali liquid is used to pretreat crushed corn straw; then a laccase and white rot fungus combined detoxification method is used to remove various phenolic compounds inhibiting fermentation micro-organism flora generated during the pretreatment process; and then neurospora is inoculated in the detoxified corn straw for fermentation to obtain the straw feed raw material which is reduced in lignin andsignificantly increased in true proteins. The straw biological feed raw material is rich in nutrients and easy to digest, and provides a new way for the high-efficient feed application of the straw.

The present invention relates to modified filamentous fungal organisms having improved activity profiles with respect to the conversion of complex carbohydrates into simple sugars from cellulosic materials, including fungal organisms belonging to a genus selected from the group consisting of: Chrysosporium, Thielavia, Talaromyces, Thermomyces, Thermoascus, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryplococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Gibberella, Myceliophthora, Mucor, Aspergillus, Fusarium, Humicola, Trichoderma, and Talaromyces, plus anamorphs and teleomorphs thereof. Filamentous fungal organisms having improved activity profiles are obtained by modifying genes encoding enzymes involved in the production of cellobionolactone, cellobionic acid, gluconolactone, gluconic acid, and related products, by a variety of mutagenic methods, resulting in nucleotide substitutions, insertions, and deletions, increasing the level of saccharification in enzyme mixtures obtained from the modified organisms.

The invention discloses a bactericide for suppressing growth of neurospora. The neurospora bactericide is prepared by performing liquid culture on a pseudomonas putida strain and centrifugally extracting supernatant from the bacterial solution. The neurospora bactericide of the invention has the characteristics of good sterilization effect, safety and the like.

An air-oxidative type hair dye composition containing a melanin precursor prepared by a process including (A) an oxidation step for converting, into the melanin precursor, a tyrosine or derivative thereof used as a starting substance with an enzyme or cell that is derived from a fungus selected from the group consisting of fungi belonging to the genera Aspergillus, Neurospora, Rhizomucor, Trichoderma, and Penicillium and that exhibits a catechol oxidase activity.

The present invention discloses one kind of neurospora preventing and controlling preparation as one kind of farm germicide. The preparation is compounded with thiophanate, hymexazol, streptomycin, Wanxiaoling, chlorothalonil and pure water. The present invention has the advantage of capacity of being applied directly onto the disease spots to kill germ effectively in condition of no isolation of disease germ hypha and to prevent and control the spread of germ.

The invention relates to a biological disease prevention insect prevention and nutrient controlled release type flower planting fertilizer and a preparation method and application thereof. The flower planting fertilizer is composed of bio-control medicine fertilizer, sulphurated high-molecular polymer sulfur coated urea, 5% recover thermoplasticity sulfur coated urea, nitrogen-phosphorus-potassium three element compound fertilizer and diammonium phosphate. The bio-control medicine fertilizer is manufactured by compounding and prilling bio-control bacterium actinomycetes, neurospora bacterium and penicillium through solid-culture prepared solid culture, urea and bentonite according to the prior art respectively. The invention further provides a preparation method and application of the flower planting fertilizer. The flower planting fertilizer is prepared by matching the bio-control medicine fertilizer with controlled release nitrogenous fertilizer, phosphatic fertilizer and potash fertilizer, provides soil borne disease and insect prevention and cure for planting date flowers, and can simultaneously prolong fertilizer efficiency.

The present compositions and methods relate to a beta-glucosidase from Neurospora crassa, polynucleotides encoding the beta-glucosidase, and methods of make and / or use thereof. Formulations containing the beta-glucosidase are suitable for use in hydrolyzing lignocellulosic biomass substrates.

The invention discloses a preparation method of a penicillium group compounded cellulose synergistic degradation compound enzyme. The compound enzyme is prepared by adding a bacterial liquid M18, which is prepared by compounding penicillium oxalicum XZH-2, penicillium XZH-6, penicillium citrinum XZH-16, penicillium XZH-22, neurospora crassa N1 and orange thermoascomycetes T2 according to a volume ratio of 1: 8: 1: 1: 1: 1, into a solid-state fermentation culture medium, and carrying out mixed fermentation. When the compound enzyme combined preparation is applied to wood cellulose enzymolysis, the enzymolysis efficiency is improved by 21.5%, and the highest cellulose conversion rate can reach 88.7%. The compound enzyme combined preparation is an oxidation hydrolase with a synergistic cellulose degradation effect, and can break glucosidic bonds of cellulose through an oxidation effect and promote combination of cellulase and a fiber substrate so as to improve the enzymolysis efficiency, so that the enzyme addition amount in the enzymolysis process is effectively reduced. The production cost of the cellulosic ethanol can be further reduced, and the industrialization process of the cellulosic ethanol is accelerated.

The invention provides a low-cost efficient pig feed. Each 100 parts by weight of feed is prepared from the following raw materials in parts by weight: 30-40 parts of straw waste, 10-20 parts of distiller's grains, 5-10 parts of bicarbonate soda, 5-25 parts of bean dregs, 0.1-0.3 part of lysine, 0.01-0.1 part of methionine, 0.05-0.12 part of threonine, and 1-3 parts of a compound microorganism preparation, wherein the strain combination of the compound microorganism preparation is selected from at least two of coriolus versicolor, aspergillus oryzae, saccharomyces cerevisiae, neurospora sitophila and bacillus subtilis. The matched feed formula provided by the invention is balanced and rich in nutrition, the interaction between multiple probiotics and the raw materials is reasonably used toobtain a good fermenting effect, the sufficient development of waste resources such as straw, bean dregs and distiller's grains is realized, the requirement of sustainable development is met, the feeding cost is reduced, the production benefits are improved, and the environmental pollution can be reduced.

The invention belongs to the technical field of cultivation of edible mushrooms, and relates to a disease resistant culture medium for mushrooms and a preparation method of the disease resistant culture medium. The disease resistant culture medium for the mushrooms comprises the following raw materials: water chestnut hull powder, mixed residue of wild jujube pits and wild jujube wood, bran, corncobs, gypsum and saccharose. The culture medium obviously reduces the diseases caused by contaminative microbes of neurospora and trichoderma viride, and effectively reduces the contamination rate; seen from a fruiting result, the coloring time is obviously shortened when the culture medium is used, the property of a produced mushroom sporocarp is excellent, and the cultivation cost of the mushrooms is obviously lowered.

The invention discloses a multi-strain composite brown rice solid-state fermentation inoculant, and a preparation method and application thereof. The multi-strain composite brown rice solid state fermentation inoculant comprises lactic acid bacteria, mould, saccharomycetes and neurospora, wherein a mass ratio of the lactic acid bacteria to the mould to the saccharomycetes to the neurospora is (2-8): (0-4): (0.1-8): (0.1-4). According to the invention, the inoculant is also used for preparing solid-state fermented brown rice-containing food, the content of soluble dietary fibers is remarkably increased and is increased by 138% compared with the content of soluble dietary fibers in non-solid-state fermented brown rice, the content of polyphenol substances is increased by 86% or above, the content of vitamin B2 and the content of vitamin E are increased by 30% and 91% respectively, and antioxidant activity is increased to 3.8 times or above of the antioxidant activity of the non-fermented brown rice; meanwhile, brown rice particles are whiter and smoother in appearance, the problem that brown rice is dark in color is solved, and taste is softer and more sticky; and a series of small-molecule flavor substances are generated through the metabolic process of different strains, so a brown rice product is endowed with unique flavor characteristics, and the taste of the brown rice product is more aromatic and rich.

The invention discloses a cultivation material capable of preventing growth of Neurospora in Pleurotas sapidus and a preparation method thereof. The cultivation material contains 89 to 92% of cottonseed shell, 2 to 5% of corn flour, 2 to 3% of wheat bran, 1 to 2% of calcium sulfate and to 2% of quicklime. The preparation method comprises the following steps: uniformly mixing the above-mentioned raw materials, adding water according to a ratio of the dry mixture to water of 1: 1.3 and adjusting a pH value to 10 to 11; piling up the culture material to form a material pile, perforating the material pile with a large stick with a diameter of 5 to 8 cm, wherein distance between holes is 40 cm, covering the material pile with a straw mat, turning the material pile over in 24 h after the temperature of the material pile reaches 55 DEG C and spraying limewash with a weight percentage of 1% onto the material pile until the water content of the material pile is 65 to 68%; turning the material pile over twice to three times, bagging the material pile after completion of fermentation, covering a bag pile with a plastic film, introducing steam into the bag pile from the center of the bottom of the bag pile so as to allow the temperature of the center of the bag pile to be 80 DEG C, maintaining the temperature for 1 h and then carrying out natural cooling so as to obtain the cultivation material. The cultivation material enables the contamination rate of a fungus sack to be decreased to 0.3% or below, prevents growth of Neurospora in Pleurotas sapidus and has wide practicality.

The invention discloses a preparation method of a biological pigment by double-strain fermentation with wastewater rich in amino acids as a base material. In the preparation method, Neurospora crassaand Auricularia auricula are used as fermentation strains, and amino acid-rich wastewater is used to prepare a fermentation medium, oscillating fermentation culture is carried out at 25-32 DEG C, andthe obtained fermentation broth is subjected to extracting and concentrating, thereby obtaining the biological pigment. According to the invention, the wastewater rich in amino acids is utilized to prepare the fermentation medium, and then double-strain coupling fermentation is utilized to produce the pigment; meanwhile, the treatment of wastewater rich in amino acids and the application of wastewater resource are efficiently combined, reducing the cost of producing the biological pigment. The produced biological pigment has the characteristics of high yield, environmental protection and strong ecological acceptability, and has a good staining effect on proteins.

Disclosed is an edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags. The method is characterized that during the production of the stock strain or culture strain, the process comprises preparing raw materials, which comprises preparing wheat grains, sawdust and cottonseed shell as culture medium for stock strain, or preparing wheat grains, sawdust and cottonseed shell as culture medium for culture strain, and preparing moisture-proof covers and plenty of cotton plugs for later use; feeding the culture medium into strain bottles or culture bags with conventional methods, inserting cotton plugs into bottle mouths or opening of the culture bags, and covering the moisture-proof covers at the bottle mouths or bag openings to form moisture-proof cover layers for preventing cotton plugs from being moistened, sterilizing the culture medium by atmospheric pressure steam sterilization or high pressure steam sterilization; and inoculating and culturing to obtain stock strain or culture strain. The method of the invention can completely prevent moisture absorption phenomenon, the operation is simple, the moisture-proof covers can be repeatedly used, the use cost is low, the problem of moisture absorption of the cotton plugs can be well solved, Neurospora growth on the cotton plugs after inoculation can be prevented, and contamination of the fungus strains is avoided.

The present compositions and methods relate to a beta-glucosidase from Neurospora crassa, polynucleotides encoding the beta-glucosidase, and methods of make and / or use thereof. Formulations containing the beta-glucosidase are suitable for use in hydrolyzing lignocellulosic biomass substrates.

The invention provides a mycocide for edible fungus production. The mycocide is prepared from IPBC (iodopropynyl butyl carbamate), xinjunan, octanohydroxamic acid, urea peroxide, ethylhexylglycerin and dipropylene glycol as a solvent. The mycocide has the advantages of being good in compatibility, good in mycete killing effect and good in stability, taking effect quickly, preventing relapse and the like, and toxicological safety is better with adoption of the non-pesticide mycocide. The mycocide can effectively kill neurospora, green molds and various infectious microbes in the edible fungus production. An application effect indicates that the mycocide is an excellent broad-spectrum and efficient mycete killing preparation with good toxicological safety.

The invention discloses a ganoderma-lucidum-karst substitute cultivation medium inhibiting neurospora. The ganoderma-lucidum-karst substitute cultivation medium inhibiting neurospora is prepared from, by weight, 40-65 parts of wood chips, 30-40 parts of auxiliary materials, 0.5-1.5 parts of gypsum, 0.5-2 parts of sugar and 0.5-1.5 parts of Chinese-mugwort-leaf and rhizome-atractylodis mixtures, and the ratio of a material to water is 1:(1.3-1.5). The ganoderma-lucidum-karst substitute cultivation medium is adopted, growth of the neurospora can be well inhibited in the cultivation process of ganoderma lucidum karst, the effect is remarkable, and meanwhile the yield of the ganoderma lucidum karst can be increased.

The invention discloses a whole-set reagent and a method for inhibiting gene expression of pine wood nematode. The whole-set reagent comprises a whole-set reagent 1 and a whole-set reagent 2, wherein the whole-set reagent 1 comprises a whole-set reagent A and nucleic acid molecules reducing expression of hsp gene, the whole-set reagent A comprises a pDE3dBH.qa carrier, a neurospora crassa dcl-1- / dcl-2 and pBT6 plasmid, and the whole-set reagent 2 comprises a whole-set reagent A and nucleic acid molecules reducing expression of cyc gene or nucleic acid molecules reducing expression of tmy gene. Experiments prove that the expression of target genes (hsp, tmy and cyc genes) of pine wood nematode can be successfully inhibited by utilizing the whole-set reagent and the method, and further high-temperature resistance sensitivity can be reduced and the movement activity can be inhibited.

The invention relates to a biological disease prevention insect prevention and nutrient controlled release type flower planting fertilizer and a preparation method and application thereof. The flower planting fertilizer is composed of bio-control medicine fertilizer, sulphurated high-molecular polymer sulfur coated urea, 5% recover thermoplasticity sulfur coated urea, nitrogen-phosphorus-potassium three element compound fertilizer and diammonium phosphate. The bio-control medicine fertilizer is manufactured by compounding and prilling bio-control bacterium actinomycetes, neurospora bacterium and penicillium through solid-culture prepared solid culture, urea and bentonite according to the prior art respectively. The invention further provides a preparation method and application of the flower planting fertilizer. The flower planting fertilizer is prepared by matching the bio-control medicine fertilizer with controlled release nitrogenous fertilizer, phosphatic fertilizer and potash fertilizer, provides soil borne disease and insect prevention and cure for planting date flowers, and can simultaneously prolong fertilizer efficiency.