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Neurospora.crassa

A technology of Neurospora robusta and strains, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of more research on molds and weak ability to decompose cellulose, so as to reduce environmental pollution and improve quality , great effect

Inactive Publication Date: 2010-12-01
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the amount of cellulase secreted by bacteria is small. Although actinomycetes can grow on cellulose, their ability to decompose cellulose is weak, so there are many studies on the ability of mold to produce cellulase.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dissolve the bacterial sample collected from the bean product workshop with distilled water, and mix it thoroughly with glass beads. After that, under aseptic conditions, use an inoculation loop to take a small amount of bacterial suspension and streak it on the selective medium for bacterial separation and purification. Using 60min final concentration of 1% diethyl sulfate and 3min UV combined mutagenesis to isolate and purify the original strain of Neurospora crassa with stable properties, and then carry out repeated primary screening and re-screening to finally obtain a strain with stable properties and production Mutagenized strains with stronger cellulase ability. Taking cellulase activity as the index, the effects of culture conditions such as fermentation temperature, initial pH, inoculum size and fermentation time on the enzyme activity produced by the bacteria were tested. Results The optimal fermentation temperature was 32°C, the initial pH was 6.0, the inocu...

Embodiment 2

[0018] Dissolve the bacterial sample collected from the bean product workshop with distilled water, and mix it thoroughly with glass beads. After that, under aseptic conditions, use an inoculation loop to take a small amount of bacterial suspension and streak it on the selective medium for bacterial separation and purification. Using 70min final concentration of 1% diethyl sulfate and 5min UV combined mutagenesis to isolate and purify the original strain of Neurospora crassa with stable properties, then repeated primary screening and re-screening, and finally obtained a strain with stable properties and production Mutagenized strains with stronger cellulase ability. Taking cellulase activity as the index, the effects of culture conditions such as fermentation temperature, initial pH, inoculum size and fermentation time on the enzyme activity produced by the bacteria were tested. Results The optimal fermentation temperature was 30°C, the initial pH was 4.8, the inoculum size w...

Embodiment 3

[0020] Dissolve the bacterial sample collected from the bean product workshop with distilled water, and mix it thoroughly with glass beads. After that, under aseptic conditions, use an inoculation loop to take a small amount of bacterial suspension and streak it on the selective medium for bacterial separation and purification. Using 60min final concentration of 1.5% diethyl sulfate and 6min UV combined mutagenesis to separate and purify the original strain of Neurospora crassa with stable traits, and then carry out repeated primary screening and secondary screening to finally obtain a strain with stable traits and production Mutagenized strains with stronger cellulase ability. Taking cellulase activity as the index, the effects of culture conditions such as fermentation temperature, initial pH, inoculum size and fermentation time on the enzyme activity produced by the bacteria were tested. Results The optimum fermentation temperature was 38°C, the initial pH was 5.0, the ino...

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PUM

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Abstract

The invention relates to Neurospora.crassa which is characterized by being named as Neurospora.crassa and is currently preserved by a preservation department designated by the State Intellectual Property Office. The preservation date is July 3rd, 2009; the preservation department is China General Microbiological Culture Collection Center; and the preservation number is CGMCC NO.3088. The invention has the advantages that: (1) a mature spore of Neurospora.crassa contains a large amount of natural beta-carotene; (2) the content of crude fiber can be greatly reduced, the quality of Neurospora.crassa is improved, and the utilization range of residues is expanded; (3) the residues of agricultural and sideline products through microbial fermentation are used, the raw material is easily acquired and is low in cost, and production equipment have low investment and great benefits; and (4) environmental pollution is reduced, the pressure of serious shortage of certain protein feeds can be alleviated, and a large amount of food is saved.

Description

technical field [0001] The present invention relates to a strain of Neurospora crassa. Background technique [0002] Food production, pollution prevention, environmental remediation, and renewable materials are the main factors affecting sustainable development. With the gradual consumption of non-renewable resources, the utilization of renewable resources has become a research hotspot, among which cellulose, as an extremely large amount of renewable resources, will become an important raw material in various fields such as food. [0003] Because cellulose usually exists in the form of insoluble fibers in a crystalline arrangement, and cellulose molecules are combined with other polysaccharides such as hemicellulose, pectin, etc., and the outside is wrapped with lignin, so people are using cellulose Prior to strong acid or alkali pretreatment, or high temperature and high pressure treatment. This process consumes a lot of energy, is expensive, and pollutes the surrounding ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/01C12R1/645
Inventor 邓泽元刘文群贾才华范亚苇李静余永红吴丹
Owner NANCHANG UNIV
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