47 results about "Neurospora crassa" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

A method for producing animal fodder by fermenting brewery mash by composite thallus is characterized in comprising: (1) drying the brewery mash, crushing, and sieving by a 20mu sieve; or crushing fresh brewery mash; (2) adjusting the material/liquid ratio by tap water, mixing well, sterilizing, and cooling for inoculation; (3)activating, and enrichment culturing the fermentation strain in total nutrient type culture medium for 24-30 hours, making preparation for inoculation; (4) placing the expanding culture thallus of neurospora crassa and lactic acid bacteria in aseptic technique condition, and inoculating on the actual production solid state fermentation culture medium according to the inoculation amount respectively in weight volume ratio of 6-8% and 8-10%; (5) placing the fermented brewery mash in the temperature of 70-80 degrees centigrade for drying, and obtaining the brewery mash fermented forage. The advantages of the invention are that: it improves the quality of brewery mash, and increases the utilization rate and digestion rate of forage; 2, reduces the investments of raw material and equipment of the microorganism fermented brewery mash forage, increases benefit; 3, reduces environmental pollution, saves a large amount of grain, and greatly reduces forage cost.

The invention discloses a gene engineering strain and an application thereof. The gene engineering strain comprises host cells and target genes transferred into the host cells, wherein the target genes include xylose reductase genes and NADPH (Nicotinamide Adenine Dinucleotide Phosphate) regenerate enzyme genes. The gene engineering strain is applied to the preparation of xylitol which is prepared through the following steps of: inoculating the engineering strain to a culture solution to culture until the OD600 is equal to 0.6-0.8, adding an inducer for inducible expression, and separating and purifying the culture solution after the inducible expression is finished. Compared with the prior art, the activity of the xylose reductase in the engineering strain is greatly improved and is 5 times as high as that of a neurospora crassa; and the gene engineering strain disclosed by the invention also contains an NADPH regeneration system, so that the xylose conversion ratio of the gene engineering strain is as high as 80.32%.

The invention discloses a bacillus amyloliquefaciens B43-1. The bacillus amyloliquefaciens B43-1 is preserved in the China General Microbiological Culture Collection Center (CCMCC) on June 8th, 2012; and the preservation number is CCMCC No.6212. A fermentation supernate of the bacillus amyloliquefaciens B43-1 can inhibit edible mushroom diseases caused by multiple pathogenic funguses such as Cladobotryum varium Nees, mucor, trichoderma, neurospora crassa and the like. The bacillus amyloliquefaciens B43-1 is nontoxic and environment-friendly to people and livestock, has a remarkable preventioneffect on edible mushroom pollution diseases, and has better biopesticide development prospects.

The invention relates to a preparation method of an inorganic heavy metal ion adsorption material, and belongs to the technical field of adsorption material preparation. The preparation method comprises the following steps: firstly, with fly ash as a raw material, preparing zeolite particles by an alkali dissolution-hydrothermal process; then, with the obtained zeolite particles as a carrier, mixing bread crumbs with glucose, egg white and water to obtain mixed slurry which serves as a nutrient solution; mixing the carrier and the nutrient solution with neurospora crassa, and fermenting; blending the fermentation filter residue with acetobacter aceti and wine, and performing secondary fermentation; finally, impregnating the secondary fermentation filter residue in alkali liquor, and naturally airing to obtain the inorganic heavy metal ion adsorption material. The heavy metal ion adsorption material prepared in the invention has the advantages of large adsorption capacity, perfect adsorbability, rare desorption and broad application prospect.

A method for producing animal fodder by fermenting brewery mash by neurospora crassa is characterized in comprising: (1) drying the brewery mash, crushing, and sieving by a 20mu sieve; or crushing fresh brewery mash; (2) adjusting the material/liquid ratio by water, mixing well, sterilizing, and cooling for inoculation; (3) activating, enrichment culturing the fermentation strain in PDA liquid culture medium for 24-48 hours, making preparation for inoculation; (4) inoculating on the production solid state fermentation culture medium according to the inoculation amount in weight volume ratio of 5-%-10%, performing fermentation and culturing for 48-98 hours; (5) placing the fermented brewery mash forage in the temperature of 70-80 degrees centigrade for drying, and obtaining the brewery mash fermented forage. The advantages of the invention is that: 1, it increases the content of beta-carotene, improves the quality of brewery mash, and increases the utilization rate and digestion rate of forage; 2, reduces the investment of raw material and production equipment, and increases benefit; 3, reduce environmental pollution, and release the pressure of serious shortage of protein forage.

The invention provides a method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa, which is characterized in that the preparation method is used for producing novel animal feed by the fermentation of neurospora crassa. The method comprises the following specific steps of: crushing and screening the tea-cake dregs with tea saponin extracted by oil; mixing the tea-cake dregs with soybean dregs or rice bran and little inorganic salt so as to be taken as a fermentation medium for production; adjusting the material-liquid ratio by water with the pH value of 7.0; subsequently carrying out heating and sterilizing treatment; subsequently carrying out cooling treatment, subsequently inoculating the activated and enriched zymocyte stains on the solid fermentation medium for production; and controlling various fermentation conditions. The method has the advantages of: 1. greatly reducing crude fiber, increasing the content of Beta-carotenes, improving the quality of tea dregs and increasing the utilization ratio and digestibility of the feed; 2. small investment for raw material and production equipment and large benefits by fermenting the tea dreg feed by microorganism; and 3. changing the waste into treasure and reducing the environmental pollution by utilizing the microorganism to ferment the tea dregs.

The invention discloses a method for preparing a protein feedstuff through straw solid-state fermentation by using neurospora crassa. According to the invention, straw is mixed with a liquid culture medium, such that a solid-state fermentation medium is prepared; and neurospora crassa is inoculated into the solid-state fermentation medium, and solid-state fermentation is carried out. The method provided by the invention has the advantages of single strain, simple process, short period, no three-waste production, and easy-to-control production process. Agricultural waste straw is adopted as the fermentation raw material, such that resource is saved, environment protection is promoted, and great economic benefit is provided. The protein feedstuff provided by the invention has high crude protein content, and is rich in lysine, threonine and vitamin B12. The feedstuff product is safe, and has no toxic or side effect. When the feedstuff is used in cooperation with other feedstuffs, a nutrition complementation effect is provided, feedstuff digestion and absorption are promoted, and feedstuff conversion rate is improved. The feedstuff can be used for partially replacing soybean meal or fishmeal in traditional feedstuffs, such that feedstuff cost can be reduced.

The invention relates to a method for extracting natural carotenoid from neurospora crassa and spores thereof, which comprises the following steps of: drying the neurospora crassa and spores thereof at 30-60 DEG C, pulverizing the culture into coarse powder, breaking walls for 30 s to 30 min by using a 0.1-3 mol/L hydrochloric acid solution through the normal temperature acid method, filtering the solution, leaching the filter residue for 1-60 min by using an organic solvent after water washing, evaporating the extracting solution at reduced pressure, and recovering the solvent to obtain coarse extract. Compared with the traditional carotenoid extraction method, the method provided by the invention has the advantages of simple process, high pigment extraction rate, low production cost, high carotenoid purity and the like.

The invention discloses an application of FvCPC2 protein and a coding gene thereof in regulating and controlling the mycelial growth and fruiting body development of various edible fungi. The overexpression and knockdown expression experiments of FvCPC2 in flammulina velutipes show that the FvCPC2 influences the growth of the flammulina velutipes mycelia in the flat plate and the culture material,and determines the formation of the flammulina velutipes primordium and the development of the fruiting body, and the FvCPC2 can be applied to the breeding of the excellent strain of the flammulina velutipes. At the same time, the FvCPC2 can replace the control function of cpc-2 mycelial growth and fruiting body development in the neurospora crassa through the replenishment experiment, which indicates that FvCPC2 is not only ubiquitous in basidiomycetes and ascomycetes, but also has the universality of the regulation and control function of fruiting body development and can be applied to thebreeding of various edible fungi. The FvCPC2 is the first discovery in the edible fungi, and is also the first application in the genetic engineering breeding of the edible fungi.

The invention provides a genetically engineered strain for high yield of ergothioneine and application. The stain takes escherichia coli as a host, and a coding gene hisG* of a corynebacterium glutamicum ATP phosphoribosyl transferase HisG mutant is integrated on a genome of the host; the copy number of a histidine operon gene hisDBCHAFI is further increased on the genome of the host; a mycobacterium smegmatis ergothioneine operon gene egtBCDE is further integrated on the genome of the host; an Escherichia coli glutamylcysteine ligase encoding gene gshA is integrated on the genome of the host,so that the synthesis of the ergothioneine is promoted; a neurospora crassa C-S lyase coding gene egtE<ncr> is further integrated on the genome of the host, so that the synthesis of ergothioneine isfurther promoted; and a gene egtB*<msm> of a sulfoxide synthase mutant is integrated on a genome of the host, so that synthesis of ergothioneine is further promoted.

The invention belongs to the field of food biotechnologies, and particularly discloses a neurospora crassa strain and application thereof. The strain is neurospora crassa and has been preserved in thegeneral microbiological center of China Committee for Culture Collection of Microorganisms, the preservation date is 16 January 2019, and the preservation number is CGMCC NO:3.19233. The neurospora crassa strain LY03 is subjected to fermentation and expanding culture and then mixed with corn flour or starch or other carriers to prepare a fermentation bacteria cake, and the fermentation bacteria cake is inoculated into bean dregs to prepare red fungus bean curd. The corn flour, starch and other ingredients which are added into fermentation bacteria cake help disperse fermentation strain hyphae, enhance the cellulase activity, reduce the content of fibers, polysaccharides and other carbohydrates in the bean dregs, increase the content of total protein in the finished product and improve thetaste. The deficiencies are overcome that in a traditional process, a fermentation strain is dependent on gifts from nature, the batches are different, the quality is unstable, and the production scale is small, the production industry chain of the soybean and auxiliary materials thereof is widened, and the neurospora crassa strain is wide in application prospect.

The invention relates to an engineering neurospora crassa, a preparation method thereof and an application of the neurospora crassa, and solves the technical problem that an existing strain cannot rapidly degrade cell walls of plants. The engineering neurospora crassa is classified and named as neurospora crassa Pcbh-2-clr2, and the collection number of the engineering neurospora crassa is CGMCC No. 18102. The engineering neurospora crassa can be widely applied to the field of branch fermentation.

The invention provides an application of fluazinam and iprodione mixed suspension to control of edible fungus Neurospora crassa derived diseases and further provides bactericidal composition for controlling the edible fungus Neurospora crassa derived diseases. The bactericidal composition contains the fluazinam and iprodione mixed suspension, and the mass ratio of fluazinam to iprodione is (1-50):(1-50). The suspension and the bactericidal composition have excellent control effect nearly being 90% on the edible fungus Neurospora crassa derived diseases under the matching of fluazinam and iprodione and the dosage of the suspension.

The invention relates to the field of adsorption material preparation, and particularly relates to an inorganic heavy metal ion adsorption material and a preparation method thereof. The inorganic heavy metal ion adsorption material is prepared from, by weight, 10 parts of primary fermentation liquor filter residues, 1 part of acetobacter aceti suspension and 5 parts of grape wine, wherein the primary fermentation material comprises the following raw materials in parts by weight: 11 parts of an inorganic zeolite carrier, 2 parts of bread crumbs, 10 parts of glucose, 1 part of neurospora crassa suspension and 20 parts of water. The preparation method of the inorganic heavy metal ion adsorption material comprises the steps of 1, mixing the raw materials, and performing primary fermentation; 2, filtering a product obtained in the step 1, taking filter residues, mixing the filter residues with other raw materials, and carrying out secondary fermentation; and 3, filtering the product obtained in the step 2, washing the filter residues with water, and naturally airing to obtain the inorganic heavy metal ion adsorption material. The invention further relates to a preparation device of the inorganic heavy metal ion adsorption material.

The invention discloses a rhodosporidium toruloides recombinant expression strain with high yield of ergothioneine as well as a construction method and application of the rhodosporidium toruloides recombinant expression strain. The method comprises the following steps: introducing and integrating ergothioneine synthesis genes egt1 and egt2 obtained by cloning from Neurospora crassa into a rhodosporidium toruloides genome, and screening to obtain a high-yield ergothioneine strain. Compared with an original strain, the yield of ergothioneine fermented by a 5L fermentation tank of the recombinant strain is increased by 270.2% compared with that of the original strain and reaches about 8.5 g/L, and a good foundation is laid for industrial production of ergothioneine by the Rhodosporidium torulosporidium strain. Meanwhile, the fermentation period is short, fermentation products are free of toxic and side effects, the metabolic pathway transformation difficulty of the rhodotorula glutinis is small, and operability is higher.

The invention specifically relates to a method for controlling the mycelial morphology of Neurospora crassa and increasing the output of cellulase, belonging to the technical field of enzyme fermentation. The mycelial morphology of Neurospora crassa in fermentation is controlled by controlling the inoculation amount of the spores of Neurospora crassa and adding talcum powder, wherein the inoculation amount of the spores of Neurospora crassa is controlled to be 2.4 * 10<6>/ml to 8.9 * 10<6>/ml, and the addition amount of the talcum powder is 0 to 0.3%. The mycelial morphology of Neurospora crassa during fermentation is controlled by controlling the inoculation amount of the spores and the addition amount of the talcum powder, so the purpose of high-efficiency production of different desired products can be achieved; by controlling the inoculation amount of the spores of Neurospora crassa and the addition amount of the talcum powder, mycelia are allowed to be in a free and disperse state, and the output of cellulase is maximized; and the method provided by the invention has the advantages of simplicity, remarkable effect, low cost, good environmental compatibility and no subsequent recovery difficulties, and can be used for large-scale production.