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47 results about "Neurospora crassa" patented technology
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Neurospora crassa is a type of red bread mold of the phylum Ascomycota. The genus name, meaning "nerve spore" in Greek, refers to the characteristic striations on the spores. The first published account of this fungus was from an infestation of French bakeries in 1843.
Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.
Owner:UNILEVER PATENT HLDG BV
Method for producing animal fodder by fermenting brewery mash by composite thallus
A method for producing animal fodder by fermenting brewery mash by composite thallus is characterized in comprising: (1) drying the brewery mash, crushing, and sieving by a 20mu sieve; or crushing fresh brewery mash; (2) adjusting the material / liquid ratio by tap water, mixing well, sterilizing, and cooling for inoculation; (3)activating, and enrichment culturing the fermentation strain in total nutrient type culture medium for 24-30 hours, making preparation for inoculation; (4) placing the expanding culture thallus of neurospora crassa and lactic acid bacteria in aseptic technique condition, and inoculating on the actual production solid state fermentation culture medium according to the inoculation amount respectively in weight volume ratio of 6-8% and 8-10%; (5) placing the fermented brewery mash in the temperature of 70-80 degrees centigrade for drying, and obtaining the brewery mash fermented forage. The advantages of the invention are that: it improves the quality of brewery mash, and increases the utilization rate and digestion rate of forage; 2, reduces the investments of raw material and equipment of the microorganism fermented brewery mash forage, increases benefit; 3, reduces environmental pollution, saves a large amount of grain, and greatly reduces forage cost.
Owner:NANCHANG UNIV
Gene engineering strain and application thereof
InactiveCN103205391AIncrease enzyme activityHigh conversion rate of xyloseBacteriaMicroorganism based processesEnzyme GeneMicrobiology
The invention discloses a gene engineering strain and an application thereof. The gene engineering strain comprises host cells and target genes transferred into the host cells, wherein the target genes include xylose reductase genes and NADPH (Nicotinamide Adenine Dinucleotide Phosphate) regenerate enzyme genes. The gene engineering strain is applied to the preparation of xylitol which is prepared through the following steps of: inoculating the engineering strain to a culture solution to culture until the OD600 is equal to 0.6-0.8, adding an inducer for inducible expression, and separating and purifying the culture solution after the inducible expression is finished. Compared with the prior art, the activity of the xylose reductase in the engineering strain is greatly improved and is 5 times as high as that of a neurospora crassa; and the gene engineering strain disclosed by the invention also contains an NADPH regeneration system, so that the xylose conversion ratio of the gene engineering strain is as high as 80.32%.
Owner:ZHEJIANG UNIV
Bacillus amyloliquefaciens and application thereof
InactiveCN102776146ASignificant effect of pollution diseaseGood prospects for the development of biopesticidesBiocideBacteriaPlant diseaseFermentation
The invention discloses a bacillus amyloliquefaciens B43-1. The bacillus amyloliquefaciens B43-1 is preserved in the China General Microbiological Culture Collection Center (CCMCC) on June 8th, 2012; and the preservation number is CCMCC No.6212. A fermentation supernate of the bacillus amyloliquefaciens B43-1 can inhibit edible mushroom diseases caused by multiple pathogenic funguses such as Cladobotryum varium Nees, mucor, trichoderma, neurospora crassa and the like. The bacillus amyloliquefaciens B43-1 is nontoxic and environment-friendly to people and livestock, has a remarkable preventioneffect on edible mushroom pollution diseases, and has better biopesticide development prospects.
Owner:NANJING AGRICULTURAL UNIVERSITY
Preparation method of inorganic heavy metal ion adsorption material
ActiveCN108404860AImprove adsorption capacityRich pore structureOther chemical processesIon adsorptionHeavy metals
Owner:王顺方
Method for producing animal fodder by fermenting brewery mash by neurospora crassa
InactiveCN101584376AImprove qualityLow costFood processingAnimal feeding stuffAnimal ForagingBeta-Carotene
A method for producing animal fodder by fermenting brewery mash by neurospora crassa is characterized in comprising: (1) drying the brewery mash, crushing, and sieving by a 20mu sieve; or crushing fresh brewery mash; (2) adjusting the material / liquid ratio by water, mixing well, sterilizing, and cooling for inoculation; (3) activating, enrichment culturing the fermentation strain in PDA liquid culture medium for 24-48 hours, making preparation for inoculation; (4) inoculating on the production solid state fermentation culture medium according to the inoculation amount in weight volume ratio of 5-%-10%, performing fermentation and culturing for 48-98 hours; (5) placing the fermented brewery mash forage in the temperature of 70-80 degrees centigrade for drying, and obtaining the brewery mash fermented forage. The advantages of the invention is that: 1, it increases the content of beta-carotene, improves the quality of brewery mash, and increases the utilization rate and digestion rate of forage; 2, reduces the investment of raw material and production equipment, and increases benefit; 3, reduce environmental pollution, and release the pressure of serious shortage of protein forage.
Owner:NANCHANG UNIV
Method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa
ActiveCN101579039AImprove qualityIncrease profitFood processingAnimal feeding stuffFiberBeta-Carotene
The invention provides a method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa, which is characterized in that the preparation method is used for producing novel animal feed by the fermentation of neurospora crassa. The method comprises the following specific steps of: crushing and screening the tea-cake dregs with tea saponin extracted by oil; mixing the tea-cake dregs with soybean dregs or rice bran and little inorganic salt so as to be taken as a fermentation medium for production; adjusting the material-liquid ratio by water with the pH value of 7.0; subsequently carrying out heating and sterilizing treatment; subsequently carrying out cooling treatment, subsequently inoculating the activated and enriched zymocyte stains on the solid fermentation medium for production; and controlling various fermentation conditions. The method has the advantages of: 1. greatly reducing crude fiber, increasing the content of Beta-carotenes, improving the quality of tea dregs and increasing the utilization ratio and digestibility of the feed; 2. small investment for raw material and production equipment and large benefits by fermenting the tea dreg feed by microorganism; and 3. changing the waste into treasure and reducing the environmental pollution by utilizing the microorganism to ferment the tea dregs.
Owner:浙江播恩生物技术有限公司
Method for preparing protein feedstuff through straw solid-state fermentation by using neurospora crassa
ActiveCN103229888AReduce crude fiber contentImprove qualityFood processingAnimal feeding stuffSide effectThreonine
The invention discloses a method for preparing a protein feedstuff through straw solid-state fermentation by using neurospora crassa. According to the invention, straw is mixed with a liquid culture medium, such that a solid-state fermentation medium is prepared; and neurospora crassa is inoculated into the solid-state fermentation medium, and solid-state fermentation is carried out. The method provided by the invention has the advantages of single strain, simple process, short period, no three-waste production, and easy-to-control production process. Agricultural waste straw is adopted as the fermentation raw material, such that resource is saved, environment protection is promoted, and great economic benefit is provided. The protein feedstuff provided by the invention has high crude protein content, and is rich in lysine, threonine and vitamin B12. The feedstuff product is safe, and has no toxic or side effect. When the feedstuff is used in cooperation with other feedstuffs, a nutrition complementation effect is provided, feedstuff digestion and absorption are promoted, and feedstuff conversion rate is improved. The feedstuff can be used for partially replacing soybean meal or fishmeal in traditional feedstuffs, such that feedstuff cost can be reduced.
Owner:SHAANXI SHIYANG AGRI TECH
Method for extracting natural carotenoid from neurospora crassa and spores thereof
The invention relates to a method for extracting natural carotenoid from neurospora crassa and spores thereof, which comprises the following steps of: drying the neurospora crassa and spores thereof at 30-60 DEG C, pulverizing the culture into coarse powder, breaking walls for 30 s to 30 min by using a 0.1-3 mol / L hydrochloric acid solution through the normal temperature acid method, filtering the solution, leaching the filter residue for 1-60 min by using an organic solvent after water washing, evaporating the extracting solution at reduced pressure, and recovering the solvent to obtain coarse extract. Compared with the traditional carotenoid extraction method, the method provided by the invention has the advantages of simple process, high pigment extraction rate, low production cost, high carotenoid purity and the like.
Owner:NANCHANG UNIV
Method for producing sauce by fermenting bean dregs
A method for producing a sauce by fermenting bean dregs is characterized in that the preparation method comprises the following steps of: (1) uniformly mixing bean pulp, bean dreg and bran according to the mass ratio of 1-10:1-10:1-5; and subsequently moistening and steaming the mixture; (2) cooling the mixture to room temperature after completing the steaming; and respectively carrying out starter propagation on aspergillus oryzae or neurospora crassa for 24-72h at the temperature of 20-40 DEG C; (3) mixing the aspergillus oryzae material and the neurospora crassa material by a proportion; and fermenting the aspergillus oryzae material and the neurospora crassa material at the temperature of 20-65 DEG C by adding salt water with the mass percentage concentration of 6-20%; (4) fermenting-ripening the soy sauce mash; subsequently carrying out three-stage oil spraying or squeezing; standing and clarifying the soy sauce mash to prepare raw soy sauce; and (5) obtaining the soy sauce product by sterilizing the raw soy sauce. The method has the advantages of: 1. improving the utilization ratio of raw material, good product quality and high nutritional value; 2. reducing the production cost of the soy sauce; and 3. improving the production efficiency.
Owner:NANCHANG UNIV
Gamma-butyrobetaine hydroxylase originated from neurospora crassa
Owner:CJ CHEILJEDANG CORP
Production of alpha-keto butyrate
InactiveUS7144715B2Easily and cheaply obtainingSimple and safe to useBacteriaMicroorganism based processesFlavorAdditive ingredient
A process of obtaining α-keto butyrate by bioconversion is disclosed. The molecule is accumulated in the medium and is apt to subsequent processing to food ingredients, such as top-note flavors. A suitable strain is a natural ilv-3 mutant Neurospora crassa strain, which is capable of accumulating α-keto butyrate in the fermentation medium. By adjusting specific medium parameters, up to 8 g / l medium of α-keto butyrate can be accumulated.
Owner:NESTEC SA
Application of FvCPC2 protein and coding gene thereof in regulating and controlling the mycelial growth and fruiting body development of various edible fungi
ActiveCN108640983APromote growthLarge biomassFungiMicroorganism based processesAgricultural scienceHypha
The invention discloses an application of FvCPC2 protein and a coding gene thereof in regulating and controlling the mycelial growth and fruiting body development of various edible fungi. The overexpression and knockdown expression experiments of FvCPC2 in flammulina velutipes show that the FvCPC2 influences the growth of the flammulina velutipes mycelia in the flat plate and the culture material,and determines the formation of the flammulina velutipes primordium and the development of the fruiting body, and the FvCPC2 can be applied to the breeding of the excellent strain of the flammulina velutipes. At the same time, the FvCPC2 can replace the control function of cpc-2 mycelial growth and fruiting body development in the neurospora crassa through the replenishment experiment, which indicates that FvCPC2 is not only ubiquitous in basidiomycetes and ascomycetes, but also has the universality of the regulation and control function of fruiting body development and can be applied to thebreeding of various edible fungi. The FvCPC2 is the first discovery in the edible fungi, and is also the first application in the genetic engineering breeding of the edible fungi.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Production of alpha-keto butyrate
InactiveCN1592788AConvenient and cheap to obtainMicroorganism based processesFermentationFlavorAdditive ingredient
A process of obtaining alpha-keto butyrate by bioconversion is disclosed. The molecule is accumulated in the medium and is apt to subsequent processing to food ingredients, such as top-note flavors. A suitable strain is a natural ilv-3 mutant Neurospora crassa strain, which is capable of accumulating alpha-keto butyrate in the fermentation medium. By adjusting specific medium parameters, up to 8 g / l medium of alpha-keto butyrate can be accumulated.
Owner:SOC DES PROD NESTLE SA
Endo beta-1,3 glucanase gene and process for cloning the same
InactiveCN1699576AImprove the level ofQuality improvementHydrolasesFermentationNucleotideGenetic engineering
The invention relates to an endo beta-1 glucanase gene and process for cloning, which belongs to the field of biological genetic engineering. the invention provides an endo-beta-1,3-glucanase glu originated from Neurospora crassa AS 3.1604, the nucleic acid sequence of the gene glu and the amino acid sequence of the corresponding protein, bacillus coli expression carrier pET28a-glu containing gene glu and the highly effective expression in bacillus coli, the invention can be applied to the construction of genetic engineering bacterium for the industrial production of the endo-beta-1,3-glucanase, and the increase of level and quality for endo-beta-1,3-glucanase by means of biofermentation.
Owner:JIANGNAN UNIV
Genetically engineered strain for producing ergothioneine and application
ActiveCN112251392AShort fermentation cycleClear genetic backgroundBacteriaStable introduction of DNAEscherichia coliMycobacterium smegmatis
The invention provides a genetically engineered strain for high yield of ergothioneine and application. The stain takes escherichia coli as a host, and a coding gene hisG* of a corynebacterium glutamicum ATP phosphoribosyl transferase HisG mutant is integrated on a genome of the host; the copy number of a histidine operon gene hisDBCHAFI is further increased on the genome of the host; a mycobacterium smegmatis ergothioneine operon gene egtBCDE is further integrated on the genome of the host; an Escherichia coli glutamylcysteine ligase encoding gene gshA is integrated on the genome of the host,so that the synthesis of the ergothioneine is promoted; a neurospora crassa C-S lyase coding gene egtE<ncr> is further integrated on the genome of the host, so that the synthesis of ergothioneine isfurther promoted; and a gene egtB*<msm> of a sulfoxide synthase mutant is integrated on a genome of the host, so that synthesis of ergothioneine is further promoted.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Method for fermentation preparation of beta-renieratene by neurospora crassa
InactiveCN101575632AShort fermentation cycleIncrease productivityMicroorganism based processesFermentationSporeOrganic solvent
The invention relates to a method for the fermentation preparation of beta-renieratene by neurospora crassa, and is characterized in that the preparation method is as follows: (1) bean dregs and rice bran of which the mass ratio is 1-8:1 are weighed; after being uniformly mixed with each other, the bean dregs and the rice bran are moistened by water and then are steamed; (2) after steaming, the mixture is cooled to room temperature and is inoculated with the neurospora crassa, and the formed compound is cultured to be 1 to 5 days under the culture condition of 20 to 50 DEG C; (3) after fermentation is accomplished, fermented culture media is dried or spore is collected at the temperature from 65 to 80 DEG C, an ultrasonic cracking instrument is used for cracking mature thalli spore, and an organic solvent is used for extracting the beta-renieratene; and (4) the finished product of beta-renieratene can be obtained after the beta-renieratene is separated, purified and dried. The method has the advantages of: 1. high product quality and yield; 2. adoption of bean dregs and rice bran used as the culture media, available raw materials and low preparation cost; and 3. short fermentation period of neurospora crassa, high preparation efficiency, easy process control and high operability.
Owner:NANCHANG UNIV
Neurospora crassa strain and application thereof
Owner:LONGYAN UNIV
Engineering neurospora crassa, preparation method thereof and application of neurospora crassa
InactiveCN110373337AShortened temperature shock periodShorten fermentation timeFungiBio-organic fraction processingCell wallFermentation
The invention relates to an engineering neurospora crassa, a preparation method thereof and an application of the neurospora crassa, and solves the technical problem that an existing strain cannot rapidly degrade cell walls of plants. The engineering neurospora crassa is classified and named as neurospora crassa Pcbh-2-clr2, and the collection number of the engineering neurospora crassa is CGMCC No. 18102. The engineering neurospora crassa can be widely applied to the field of branch fermentation.
Owner:威海惟农生物科技有限公司
Broad specificity DNA damage endonuclease
InactiveUS7060455B1Simple structurePeptide/protein ingredientsHydrolasesInsertion deletionSchizosaccharomyces pombe
The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, insertion deletion loops, cisplatin adducts and other types of base damage (for example, uracil resulting from cytosine deamination). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, Bacillus anthracis, Methanococcus jannaschii, and Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.
Owner:EMORY UNIVERSITY
Application of fluazinam and iprodione mixed suspension to control of edible fungus Neurospora crassa derived diseases
InactiveCN107372563AGood control effectLower resistanceBiocideDead animal preservationBiotechnologyDisease
The invention provides an application of fluazinam and iprodione mixed suspension to control of edible fungus Neurospora crassa derived diseases and further provides bactericidal composition for controlling the edible fungus Neurospora crassa derived diseases. The bactericidal composition contains the fluazinam and iprodione mixed suspension, and the mass ratio of fluazinam to iprodione is (1-50):(1-50). The suspension and the bactericidal composition have excellent control effect nearly being 90% on the edible fungus Neurospora crassa derived diseases under the matching of fluazinam and iprodione and the dosage of the suspension.
Owner:山西奥赛诺生物科技有限公司
Inorganic heavy metal ion adsorption material and preparation method thereof
ActiveCN112774304AImprove production efficiencyEasy to useBioreactor/fermenter combinationsBiological substance pretreatmentsEthylic acidPhysical chemistry
The invention relates to the field of adsorption material preparation, and particularly relates to an inorganic heavy metal ion adsorption material and a preparation method thereof. The inorganic heavy metal ion adsorption material is prepared from, by weight, 10 parts of primary fermentation liquor filter residues, 1 part of acetobacter aceti suspension and 5 parts of grape wine, wherein the primary fermentation material comprises the following raw materials in parts by weight: 11 parts of an inorganic zeolite carrier, 2 parts of bread crumbs, 10 parts of glucose, 1 part of neurospora crassa suspension and 20 parts of water. The preparation method of the inorganic heavy metal ion adsorption material comprises the steps of 1, mixing the raw materials, and performing primary fermentation; 2, filtering a product obtained in the step 1, taking filter residues, mixing the filter residues with other raw materials, and carrying out secondary fermentation; and 3, filtering the product obtained in the step 2, washing the filter residues with water, and naturally airing to obtain the inorganic heavy metal ion adsorption material. The invention further relates to a preparation device of the inorganic heavy metal ion adsorption material.
Owner:宁波美成生物科技有限公司
Rhodosporidium toruloides recombinant expression strain with high yield of ergothioneine and construction method and application of rhodosporidium toruloides recombinant expression strain
PendingCN114277042AShort fermentation cycleLow difficulty in transformationFungiMicroorganism based processesBiotechnologyMicrobiology
The invention discloses a rhodosporidium toruloides recombinant expression strain with high yield of ergothioneine as well as a construction method and application of the rhodosporidium toruloides recombinant expression strain. The method comprises the following steps: introducing and integrating ergothioneine synthesis genes egt1 and egt2 obtained by cloning from Neurospora crassa into a rhodosporidium toruloides genome, and screening to obtain a high-yield ergothioneine strain. Compared with an original strain, the yield of ergothioneine fermented by a 5L fermentation tank of the recombinant strain is increased by 270.2% compared with that of the original strain and reaches about 8.5 g / L, and a good foundation is laid for industrial production of ergothioneine by the Rhodosporidium torulosporidium strain. Meanwhile, the fermentation period is short, fermentation products are free of toxic and side effects, the metabolic pathway transformation difficulty of the rhodotorula glutinis is small, and operability is higher.
Owner:INNER MONGOLIA KINGDOMWAY PHARMA LTD +2
Method for preparing protein feed by solid-state fermentation of rice straw with Neurospora crassa
ActiveCN103229888BImprove protectionHigh activityFood processingAnimal feeding stuffBiotechnologySide effect
The invention discloses a method for preparing a protein feedstuff through straw solid-state fermentation by using neurospora crassa. According to the invention, straw is mixed with a liquid culture medium, such that a solid-state fermentation medium is prepared; and neurospora crassa is inoculated into the solid-state fermentation medium, and solid-state fermentation is carried out. The method provided by the invention has the advantages of single strain, simple process, short period, no three-waste production, and easy-to-control production process. Agricultural waste straw is adopted as the fermentation raw material, such that resource is saved, environment protection is promoted, and great economic benefit is provided. The protein feedstuff provided by the invention has high crude protein content, and is rich in lysine, threonine and vitamin B12. The feedstuff product is safe, and has no toxic or side effect. When the feedstuff is used in cooperation with other feedstuffs, a nutrition complementation effect is provided, feedstuff digestion and absorption are promoted, and feedstuff conversion rate is improved. The feedstuff can be used for partially replacing soybean meal or fishmeal in traditional feedstuffs, such that feedstuff cost can be reduced.
Owner:SHAANXI SHIYANG AGRI TECH
Method for controlling mycelial morphology of Neurospora crassa and increasing output of cellulase
InactiveCN107384848AChange shapeIncrease productionFungiMicroorganism based processesBiotechnologySpore
The invention specifically relates to a method for controlling the mycelial morphology of Neurospora crassa and increasing the output of cellulase, belonging to the technical field of enzyme fermentation. The mycelial morphology of Neurospora crassa in fermentation is controlled by controlling the inoculation amount of the spores of Neurospora crassa and adding talcum powder, wherein the inoculation amount of the spores of Neurospora crassa is controlled to be 2.4 * 10<6> / ml to 8.9 * 10<6> / ml, and the addition amount of the talcum powder is 0 to 0.3%. The mycelial morphology of Neurospora crassa during fermentation is controlled by controlling the inoculation amount of the spores and the addition amount of the talcum powder, so the purpose of high-efficiency production of different desired products can be achieved; by controlling the inoculation amount of the spores of Neurospora crassa and the addition amount of the talcum powder, mycelia are allowed to be in a free and disperse state, and the output of cellulase is maximized; and the method provided by the invention has the advantages of simplicity, remarkable effect, low cost, good environmental compatibility and no subsequent recovery difficulties, and can be used for large-scale production.
Owner:NORTHWEST A & F UNIV
Ruminant fermented feed and production method thereof
InactiveCN111328921ALess crude fiberImprove palatabilityFood processingAnimal feeding stuffMilk cow'sNutrition
The invention relates to the technical field of feed, in particular to ruminant fermented feed produced from residual fermentation liquor of molasses yeast, crushed particles of corncobs and Neurospora crassa and a production method of the ruminant fermented feed produced from the residual fermentation liquor of the molasses yeast, the crushed particles of the corncobs and the Neurospora crassa. Asolid fermented feed carrier is fermented with the Neurospora crassa to obtain the ruminant fermented feed, wherein the solid fermented feed carrier is obtained after the residual fermentation liquorof the molasses yeast, the corncobs and bran are subjected to adsorption treatment. According to the feed, the residual fermentation liquor of the molasses yeast and the corncobs are turned into wealth, and not only is the production cost low, but also the produced fermented feed has few crude fibers, is good in palatability and rich in nutrient, and can have the efficacy of easing oxidative stress, reducing somatic cells in milk and preventing subclinical mastitis when fed to dairy cows.
Owner:SHENYANG RES INST OF CHEM IND
Microorganism of Enterobacteriacae Genus Haboring Genes Associated With L-Carnitine Biosynthesis and Method of Producing L-Carnitine Using the Microorganism
InactiveUS20080171368A1Increase production capacityImprove efficiencyBacteriaFermentationS-Adenosyl-l-methioninePolynucleotide
Provided is a microorganism that belongs to Enterobacteriacae and a method of producing L-carnitine using the same. The microorganism includes polynucleotide encoding activity of S-adenosylmethionine-6-N-iysine KDa methyltransferase from Neurospora crassa, polynucleotide encoding activity of 6-N-trimethyllysine hydroxylase, polynucleotide encoding activity of 3-hydroxy-6-N-trimethyllysine aldolase, and polynucleotide encoding activity of γ-trimethylaminoaldehyde dehydrogenase and y-butyrobetaine hydroxylase.
Owner:CJ CHEILJEDANG CORP
Transformer capable of reducing sulfur corrosion
ActiveCN111662741AShort adsorption timeImprove efficiencyMicroorganismsMicroorganism based processesPhysical chemistryEngineering
The invention relates to a transformer capable of reducing sulfur corrosion provided by the invention, a novel desulfurizing agent is added into transformer oil, so that the effect of efficient and deep desulfurization is achieved, and corrosion of sulfide to transformer parts is reduced. According to the invention, neurospora crassa and candida utilis are adopted to ferment the licorice waste residue; a part of lignin, cellulose and hemicellulose in the liquorice waste residues can be degraded, mycelia of neurospora crassa and candida utilis can fully penetrate through the interior of the fermentation material, a certain physical damage effect is exerted on the mycelia, and a microcosmic cavity is formed in the material. Therefore, the space structure of the modified liquorice waste residues is destroyed, the liquorice waste residues become rough and pleated, the porosity is high, the specific surface area is increased, and the adsorption performance on sulfide is greatly improved. The transformer has the advantages that dibenzyl disulfide and dodecanethiol in the transformer can be adsorbed at the same time, the adsorption efficiency is 95% or above, and negative influences on electrical parameters such as breakdown voltage, dielectric loss and resistivity of transformer oil are avoided.
Owner:刘云
Method for preparing biochrome by double-bacteria fermentation using waste water rich in amino acid as base material
InactiveCN110029131BImprove dyeing effectIncrease productionFungiMicroorganism based processesBiotechnologyFermentation broth
The invention discloses a method for preparing biological pigments by dual-bacteria fermentation using waste water rich in amino acids as a base material. The method uses Neurospora crassa and Auricularia auricula as fermentation strains, prepares a fermentation medium with waste water rich in amino acids, vibrates and ferments at 25-32°C, and extracts and concentrates the obtained fermentation liquid to obtain Biochrome. The invention utilizes amino acid-rich waste water to prepare a fermentation medium, and then utilizes dual-species coupled fermentation to produce pigments, effectively combines the treatment of amino acid-rich waste water and waste water resource utilization, and reduces the cost of producing biological pigments. The bio-pigment produced has the characteristics of high yield, environmental protection, strong ecological acceptability, etc., and has a good dyeing effect on protein.
Owner:优贝佳(青岛)生物科技有限公司
A strain of Neurospora crassa and its application in the development of feed protein
The invention belongs to the technical field of fermented meal feed and the application of single-cell protein from low-value raw material sources, and relates to a strain of Neurospora crassa and its mixed application. In the present invention, Neurospora crassa is a wild strain isolated from decaying wood microorganisms, and through ARTP mutagenesis, its ability to degrade biomass materials is improved, and a strain of Neurospora crassa W3 is obtained. Neurospora crassa W3 is shaken in a liquid state. After bottle fermentation for 5 days, the cellulase activity can reach 9.5IU / ml, and the xylanase activity can reach 186.4IU / ml. The invention also provides a method for producing single-cell protein by mixed solid-state fermentation of Neurospora crassa W3 and Aspergillus niger 60B‑3DW, which greatly improves the quality and nutritional value of meal feed. Therefore, the present invention can be applied industrially and has good industrialization prospect.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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