Method for producing animal fodder by fermenting brewery mash by neurospora crassa

A technology of Neurospora stouti and animal feed, which is applied in the directions of animal feed, animal feed, additional food elements, etc., can solve the problems of low degradation rate of crude fiber and unfavorable absorption of nutrients, so as to reduce crude fiber and improve crude protein. Content, the effect of saving food

Inactive Publication Date: 2009-11-25
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Some people use Aspergillus niger, Aspergillus oryzae or Geotrichum candidum to produce animal feed, but the degradation rate of cr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The fresh brewer's grains produced by the brewery are dried and crushed, passed through a 20-mesh sieve, and 7.0 g of crushed brewer's grains and 2.0 g of bean dregs are weighed, (NH 4 ) 2 SO 4 1.0 g is used as the actual production medium, and is subjected to dry heat treatment at 90°C to 100°C for 15s to 1min, and the ratio of solid to liquid is adjusted to 1:1 with water. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultured for 24h-48h. Afterwards, the enriched Neurospora brassica is inserted into the actual production solid-state fermentation medium to ferment for 48 hours to 98 hours according to the inoculum amount of 5% to 10%, and the temperature is adjusted to 20°C to 40°C. After the fermentation is finished and dried, the brewer's grain fermented feed is obtained. The degradation rate of crude fiber of brewer's grains fermented by Neurospora crassa was determined to be 45%, the increase rate of crude protein to b...

Embodiment 2

[0015] The fresh brewer's grains produced by the brewery are pulverized, and the pulverized brewer's grains 68g, okara 19g, (NH 4 ) 2 SO 4 13g is used as the actual production medium, sterilized by high-temperature steam for 3 minutes to 10 minutes, and after cooling, adjust the material-liquid ratio to 1:1 with water of pH 7.0 to be inoculated. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultivated for 24h-60h. Afterwards, the enriched Neurospora brassica is inserted into the actual production solid-state fermentation medium to ferment for 48 hours to 98 hours according to the inoculum amount of 8% to 12%, and the temperature is adjusted to 25°C to 45°C. After the fermentation is finished and dried, the brewer's grain fermented feed is obtained. The degradation rate of crude fiber of brewer's grains fermented by Neurospora crassa was determined to be 47%, the increase rate of crude protein was 42%, and the yield of β-carotene wa...

Embodiment 3

[0017] The fresh brewer's grains produced by the brewery are crushed, and the crushed brewer's grains 6.5Kg, rice bran 1.7Kg, (NH 4 ) 2 SO 4 1.0Kg, KH 2 PO 4 0.8Kg is used as the actual production medium, sterilized by high-temperature steam for 3 minutes to 10 minutes, and after cooling, adjust the material-liquid ratio to 1:2.5 with water of pH 7.0 to be inoculated. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultivated for 48h-72h. Afterwards, the enriched Neurospora brassica is inserted into the actual production solid-state fermentation medium to ferment for 98 hours to 120 hours according to the inoculum amount of 5% to 10%, and the temperature is adjusted to 25°C to 50°C. After the fermentation is finished and dried, the brewer's grain fermented feed is obtained. The degradation rate of crude fiber of brewer's grains fermented by Neurospora crassa was determined to be 43%, the increase rate of crude protein to be 37%, a...

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PUM

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Abstract

A method for producing animal fodder by fermenting brewery mash by neurospora crassa is characterized in comprising: (1) drying the brewery mash, crushing, and sieving by a 20mu sieve; or crushing fresh brewery mash; (2) adjusting the material/liquid ratio by water, mixing well, sterilizing, and cooling for inoculation; (3) activating, enrichment culturing the fermentation strain in PDA liquid culture medium for 24-48 hours, making preparation for inoculation; (4) inoculating on the production solid state fermentation culture medium according to the inoculation amount in weight volume ratio of 5-%-10%, performing fermentation and culturing for 48-98 hours; (5) placing the fermented brewery mash forage in the temperature of 70-80 degrees centigrade for drying, and obtaining the brewery mash fermented forage. The advantages of the invention is that: 1, it increases the content of beta-carotene, improves the quality of brewery mash, and increases the utilization rate and digestion rate of forage; 2, reduces the investment of raw material and production equipment, and increases benefit; 3, reduce environmental pollution, and release the pressure of serious shortage of protein forage.

Description

technical field [0001] The invention relates to a method for producing animal feed by fermenting brewer's grains with Neurospora crassa. Background technique [0002] my country's beer industry is developing rapidly, increasing at an average annual rate of about 6%, and beer production ranks among the top in the world. At the same time, the output of brewer's grains is also quite abundant, and its recycling is of great significance. In foreign countries, the comprehensive utilization of brewer's grains is highly valued and has been widely used in feed, medicine, food and other industries. Our country started late, and these distiller's grains have not been well utilized. With the shortage of feed resources and the enhancement of environmental protection awareness, it is required that the feed industry should simultaneously increase income and save food, and brewing enterprises should consider both sewage discharge standards and turning waste into treasure. Therefore, in r...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K1/16A23K10/38A23K20/105A23K20/147
CPCY02P60/87
Inventor 邓泽元贾才华范亚苇李静刘蓉
Owner NANCHANG UNIV
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