Method for producing animal fodder by fermenting brewery mash by neurospora crassa
A technology of Neurospora stouti and animal feed, which is applied in the directions of animal feed, animal feed, additional food elements, etc., can solve the problems of low degradation rate of crude fiber and unfavorable absorption of nutrients, so as to reduce crude fiber and improve crude protein. Content, the effect of saving food
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Embodiment 1
[0013] The fresh brewer's grains produced by the brewery are dried and crushed, passed through a 20-mesh sieve, and 7.0 g of crushed brewer's grains and 2.0 g of bean dregs are weighed, (NH 4 ) 2 SO 4 1.0 g is used as the actual production medium, and is subjected to dry heat treatment at 90°C to 100°C for 15s to 1min, and the ratio of solid to liquid is adjusted to 1:1 with water. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultured for 24h-48h. Afterwards, the enriched Neurospora brassica is inserted into the actual production solid-state fermentation medium to ferment for 48 hours to 98 hours according to the inoculum amount of 5% to 10%, and the temperature is adjusted to 20°C to 40°C. After the fermentation is finished and dried, the brewer's grain fermented feed is obtained. The degradation rate of crude fiber of brewer's grains fermented by Neurospora crassa was determined to be 45%, the increase rate of crude protein to b...
Embodiment 2
[0015] The fresh brewer's grains produced by the brewery are pulverized, and the pulverized brewer's grains 68g, okara 19g, (NH 4 ) 2 SO 4 13g is used as the actual production medium, sterilized by high-temperature steam for 3 minutes to 10 minutes, and after cooling, adjust the material-liquid ratio to 1:1 with water of pH 7.0 to be inoculated. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultivated for 24h-60h. Afterwards, the enriched Neurospora brassica is inserted into the actual production solid-state fermentation medium to ferment for 48 hours to 98 hours according to the inoculum amount of 8% to 12%, and the temperature is adjusted to 25°C to 45°C. After the fermentation is finished and dried, the brewer's grain fermented feed is obtained. The degradation rate of crude fiber of brewer's grains fermented by Neurospora crassa was determined to be 47%, the increase rate of crude protein was 42%, and the yield of β-carotene wa...
Embodiment 3
[0017] The fresh brewer's grains produced by the brewery are crushed, and the crushed brewer's grains 6.5Kg, rice bran 1.7Kg, (NH 4 ) 2 SO 4 1.0Kg, KH 2 PO 4 0.8Kg is used as the actual production medium, sterilized by high-temperature steam for 3 minutes to 10 minutes, and after cooling, adjust the material-liquid ratio to 1:2.5 with water of pH 7.0 to be inoculated. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultivated for 48h-72h. Afterwards, the enriched Neurospora brassica is inserted into the actual production solid-state fermentation medium to ferment for 98 hours to 120 hours according to the inoculum amount of 5% to 10%, and the temperature is adjusted to 25°C to 50°C. After the fermentation is finished and dried, the brewer's grain fermented feed is obtained. The degradation rate of crude fiber of brewer's grains fermented by Neurospora crassa was determined to be 43%, the increase rate of crude protein to be 37%, a...
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