256 results about "Geotrichum" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention mainly relates to a method for preparing a protein feed by fermentation on straw and cake using aerobic mixed bacteria. The method for preparing a protein feed by fermentation on straw and cake using aerobic mixed bacteria is mainly characterized by comprising the following steps of: uniformly stirring straw, cake, wheat bran and corn flour, and enabling the water content thereof toachieve 50-60%; adding ammonium sulphate having an amount of 0.5-4% relative to the total amount of dry materials and urea having an amount of 0.5-3% relative to the total amount of dry materials; adjusting pH to 5.5-6.5 by 1% lime water; joining the raw materials in bacterial solution having an amount of 8-12% relative to the total amount of dry materials, obtained by mixing geotrichum candidum,lactic acid bacteria and bacillus licheniformis, and having a ratio by weight of 50-60% of geotrichum candidum to 30-35% of lactic acid bacteria to 5-10% of bacillus licheniformis; and fermenting for18-24 hours in a ventilation condition at a temperature of 28-32 DEG C, so as to prepare the fermented protein feed. The method for preparing a protein feed by fermentation on straw and cake using aerobic mixed bacteria disclosed by the invention utilizes modern bio-technology, optimizes the best combined bacteria, has simple and convenient production process, saves equipments and energies, and reduces the needed investment.

The invention discloses a mixed feed for sea-bass fish and a preparation method thereof. The mixed feed is made from the following raw materials in parts by weight: 35 to 45 of insect powders, 20 to 30 of a compound fermented material, 0.5 to 1 of fish oil, 10 to 20 of flour, 1.5 of calcium dihydrogen phosphate, 2 of a vitamin-mineral salt mixture, and 0.5 of choline chloride, wherein the compound fermented material is a fermented product of castor cake, rape cake and cotton cake with compound dry microbial inocula. The mixed feed is prepared by the steps as follows: mixing castor cake, rape cake and cotton cake according to a weight ratio of 1:1:1, and adding the compound dry microbial inocula composed of at least two of lactic acid bacteria, saccharomycetes, bacillus and geotrichum candidumlink strain for fermentation 2-24h, wherein the additive amount of the compound dry microbial inocula is 1% of the total weight of the fermentation raw material; and thoroughly mixing the raw materials, compounding, pelletizing and oven-drying to obtain the final product. The preparation method is simple and low in cost. The mixed feed for sea-bass fish is good in palatability and nutrition and can enhance disease resistance and stress resistance of sea-bass fish.

The invention relates to a barton fermentation bed complex microbial inoculant and a preparation method thereof. The invention is characterized in that the complex microbial inoculant comprises the following six beneficial microorganisms in percentage by weight: 15-30 percent of Trichoderma koningii, 10-25 percent of geotrichum candidum, 10-25 percent of Candida utilis, 10-30 percent of lactobacillus plantarum, 10-25 percent of bacillus subtilis and 5-15 percent of bacillus subtilis natto. The complex microbial inoculant is prepared by a fermentation process, and the total number of fermentation viable bacteria in finished products is 2-5*10<9>cfu/ml. The microbial strains adopted by the complex microbial inoculant are safe and reliable, the finished viable bacteria content is high and the bioactivity is high; 1L/m<3> complex microbial inoculant is used on a barton fermentation bed, thus obviously reducing the odor in a barton, decreasing the breeding of mosquitoes and flies, having stronger degradation capability on chicken excrement, achieving the ecologically breeding purposes of no pollution and zero emission, enhancing the digestive absorption function of chicken, improving the chicken immunity, enhancing the broiler quality, increasing the quality of eggs, and ensuring the color of yolks to be brightly red.

The invention provides a pigsty fermenting bed compound microbial fertilizer and a preparation method thereof. The method comprises: first, five strains of bulk starters which are bacillus subtilis, lactobacillus plantarum, candida utili, geotrichum candidum and aspergillus oryzae, and the viable count of the bulk starters is more than or equal to 10<9>cfu/ml; protective agent with proper amount is added into the five strains of bulk starters for freeze drying; according to the parts by weight of the freeze-dried bacteria powder, 2-4 parts of bacillus subtilis powder, 3-5 parts of lactobacillus plantarum powder, 3-5 parts of candida utili powder, 2-4 parts of geotrichum candidum powder and 3-5 parts of aspergillus oryzae powder are fully mixed together and then packaged by vacuum, so that the compound microbial fertilizer is obtained. When 5-20g/m<2> of the compound microbial fertilizer is used in the pigsty fermenting bed, the odor of the pigsty can be obviously reduced, the growth chance of flies can be decreased and the sick frequency of the pigs can be reduced, so that the pigs can grow up healthily and green pork can be provided for the human beings while the environment is protected.

The invention discloses a method for producing a protein feed through hypsizygus marmoreus fungus chaff solid state fermentation and the protein feed being produced via the method for producing the protein feed through hypsizygus marmoreus fungus chaff solid state fermentation. According to the method, hypsizygus marmoreus is selected to cultivate the produced waste material-pollution-free hypsizygus marmoreus fungus chaff, the ground hypsizygus marmoreus is combined with bran, urea and the like, candida utilis, candida tropicalis and geotrichum candidum are inoculated according to a certain rate so as to ferment under an appropriate condition, and the feed which is rich in protein is obtained. After assay determination, the protein feed contains 20%-40% of crude protein which is increased by 1.5-4 times compared with the crude protein of unfermented hypsizygus marmoreus fungus chaff; the crude fiber content is between 15% and 18% which is reduced by 2-3.5 times of the crude fiber content of the unfermented hypsizygus marmoreus fungus chaff, so that the protein feed is a hypsizygus marmoreus fungus chaff protein feed which has rich nutrition, high protein and low fiber and is beneficial to the utilization and absorption of livestocks. By the method, the feeding cost of farmers can be lowered, waste utilization is realized, and an environmental protection function is obtained.

The invention relates to a special fertilizer for Hami melon, which is an organic-inorganic compound fertilizer consisting of enzymolysis oil residue, ammonium sulfate, diammonium phosphate, potassium sulfate, magnesium sulfate, zinc sulfate and boric acid, wherein the enzymolysis oil residue is a product produced by utilizing lipase in bacillus subtilis zymotic fluid and yarrowia lipolytica zymotic fluid and protease in geotrichum candidum zymotic fluid and lactobacillus plantarum zymotic fluid to carry out biodegradation on oil residue. The special fertilizer is specially configured against the absorption rules of nutrients of Xinjiang Hami melon and the nutrient status of the soil of a main producing region of the Hami melon, and can fully utilize the special nitrogen nutrient of the oil residue, increase the output and improve the quality.

The invention relates to a special fertilizer for Zhanhua winter jujubes, a preparation method and the application thereof. The special fertilizer for Zhanhua winter jujubes comprises the following raw materials in parts by mass: 50 to 70 parts of zymolysing oil residues, 10 to 20 parts of urea, 5 to 15 parts of DAP (diammonium phosphate), 10 to 18 parts of potassium sulfate, 0.5 to 1 part of ferrous sulphate, 0.3 to 0.7 part of zinc sulfate and 0.2 to 0.4 part of boric acid, wherein the zymolysing oil residue is a product produced by carrying out biodegradation on the oil residues by using lipases in bacillus subtilis fermentation broth and yarrowia lipolytica fermentation broth and proteases in geotrichum candidum fermentation broth and lactobacillus plantarum fermentation broth. The special fertilizer of the invention is prepared especially according to the nutrient status of soils in the main producing areas of Zhanhua winter jujubes and the regulation of nutrient absorption of the jujubes, and has the advantages of reasonable nutrient ratio and strong pertinence. The special fertilizer can reduce the salt damages caused by fertilizing and the organic and inorganic combination can improve the soil fertility.

The invention discloses a method for preparing a microorganism fermentation feed. The method comprises the main technological processes as follows: respectively culturing Candida utilis, Geotrichum candidum and Streptococcus faecalis with molasses in combination with a liquid microorganism culture medium as the main raw materials; mixing the Candida utilis culture solution, the Geotrichum candidum culture solution and the Streptococcus faecalis culture solution at a certain ratio to obtain a mixed bacteria solution; subjecting to solid fermentation in a semi-solid fermentation culture medium including molasses liquefied liquid, corn coat, vegetable meal, cotton seed meal, wheat bran, soybean meal and apple pomace; baking at a low temperature when the fermentation is finished; and pulverizing to obtain the microorganism fermentation feed. In the microorganism fermentation feed, the viable count of beneficial microorganism is larger than 1*10<9>cfu/g, and the phytase activity is higher than 1,400IU/kg. The feed has the functions of supplementing the number of milk cow (sheep) rumen microorganism, regulating rumen fermentation function, and improving feed conversion and utilization ratio.

The invention belongs to the technical field of microbial fermentation, and concretely relates to a method for preparing a distillers' grain protein feed through three-stage mixed bacterium solid state fermentation. The method comprises the following steps: 1, first stage fermentation: carrying out high temperature fermentation on an A group bacillus combined seed liquid at 60DEG C with distillers' grains as a substrate to obtain a distillers' grain fermentation material; 2, adding locust tree leaf powder, poplar tree leaf powder and wheat bran to the distillers' grain fermentation material obtained after the first stage fermentation, inoculating a Geotrichum candidum, yeast and Trichoderma spp. seed liquid, and blending to obtain a fermentation material, and carrying out second stage fermentation; and 3, inoculating a final product obtained after the second stage fermentation to a B group bacillus combined seed liquid, carrying out third stage fermentation to obtain a final fermentation product fresh distillers' grain protein feed, or drying, crushing, granulating and packaging to obtain a particulate distillers' grain protein feed. The content of crude proteins in the distillers' grain protein feed prepared by adopting the method is 29-32% averagely, and is 58-74% higher than the content of crude proteins in dry distillers' grains.

The invention belongs to the technical field of biological fermentation, and mainly relates to the field of recycling of citrus pulp wastes and the field of biological feed. Technical points of a leavening agent are as follows: choosing aspergillus niger, geotrichum candidum and rice wine yeast which are appropriate for growing on the citrus pulp; resuscitating and respectively enlarging a solid culture which is used for producing seed bacteria, and dewatering and drying at low temperature; and mixing uniformly according to the content of microorganisms in the solid seed bacteria to form a composite solid biological leavening agent containing enough active rice wine saccharomycetes, aspergillus niger spores and geotrichum candidum spores. A product obtained by fermenting the citrus pulp by using the composite leavening agent is a good feed material; the inoculant is low in cost and convenient to use; enterprises just need to proportion solid matrix materials of the citrus pulp in combination of actual production, add a proper amount of the composite leavening agent and culture at proper temperature to obtain a qualified fermented product; and the use of the fermented product as the feed material can save the grain and increase the economic benefit of the breeding industry.

The invention discloses a universal feed starter composed of the following raw materials by weight: 20-30 parts of soybean meal, 20-30 parts of corn, 20-30 parts of straw, 10-20 parts of fish meal, 10-20 parts of bran, 5-10 parts of sugar, 5-10 parts of Omega-3 fatty acid, 100 parts of multidimensional composite enzyme bacteria and 50-100 parts of water; the multidimensional composite enzyme bacteria contains 5-10 parts of bcillus subtilis, 5-1 parts of bacillus licheniformis, 10-20 parts of acetobacter aceti, 10-20 parts of lactobacillus amylovorus, 5-10 parts of candida, 10-20 parts of protease, 5-10 parts of geotrichum candidum, and balance of enterococcus faecalis. The efficient feed starter prepared by fermentation of various raw materials by the multidimensional composite enzyme bacteria can be widely used in breeding of ruminant animals, swine, poultry, aquatics and the like. The efficient feed starter fermentation agent belongs to a micro ecological preparation, does not contain any chemical agents and hormones, and is used in the production of green livestock products and aquatic products beneficial to human health.

The invention mainly relates to a method for preparing protein feed from straw and inedible vegetables through mixed bacteria aerobic fermentation. The method is mainly characterized by comprising the following steps: uniformly stirring straw, inedible vegetables, rapeseed meal and bran till the moisture content reaches 50-60 percent; adding ammonium sulfate the mass of which accounts for 2-6 percent of the total mass of the dry materials, and urea the mass of which accounts for 1-4 percent of the total mass of the dry materials; regulating the pH value to be 5.5-6.5 by using 1 percent of whitewash; inoculating bacteria liquor which is formed by mixing Geotrichum candidum Link, lactic acid bacteria and aspergillus niger and the mass of which accounts for 8-12 percent of the total mass of the dry materials, wherein the mixed bacteria liquor comprises the following components in percentage by weight: 50-70 percent of Geotrichum candidum Link, 20-25 percent of lactic acid bacteria and 5-10 percent of aspergillus niger; fermenting at the temperature of 28-32 DEG C under ventilation conditions for 24-36 hours, thereby preparing the fermented protein feed. According to the method, by utilizing the modern biotechnology, optimal combined bacteria are optimized, the production process is simple and convenient, equipment and energy are saved, and the needed investment is low.

An active yeast feed rich in enzymes is prepared from the geotrichum candidum link, candida utilis, candida arborea, saccharomyces, cerevisiae, etc through respectively preparing their leavening agents, respectively inoculating then in solid fermenting culture media, fermenting culture at 33-35 deg.C for 28-30 hr, and drying at 38-45 deg.C.

The invention is a compound microbial inoculant that processes human and animal excreta effectively composed of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcus thermophilus, geotrichum candidum, aspergillus niger and thermal amylase streptomycete. The microbial inoculum uses metabolism to decompose human and animal excreta effectively in short period of time effectively without secondary pollution during process, which extremely reduces environmental pollution brought by human and animal excreta. Meanwhile, the method is sample, low in cost, and saves resources, which can be widely applied to fixed or movable rest rooms, matching the policies of sustainable development.

The invention discloses a preparation method of soft cheese and belongs to the field of dairy food processing. The method comprises the following steps: performing standardization; performing pasteurization; fermenting lactic acid bacteria; performing chymosin curding; discharging whey; performing molding; inoculating monascus firstly, and after a time, inoculating a mixed fungus liquid of geotrichum canididum and camembert blue mould, or inoculating monascus and the mixed fungus liquid of geotrichum canididum and camembert blue mould at one step; selecting proper ripening conditions. According to the method, monascus and white mould are mixed and ripened to prepare white mould soft cheese, so that the cheese is permeated with certain monascus color, can effectively improve the heavy flavor and taste of traditional white mould cheese, has good plasticity and is suitable for Chinese to eat.

The invention belongs to the technical field of wastewater treatment and particularly relates to a microbial agent suitable for treating urban wastewater and a preparation method thereof. The microbial agent comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, saccharomycetes, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria, phosphorus-accumulating bacteria, Rhodospirillum, Thiobacillus denitrificans, Sphingobacteria, Alcaligenes faecalis, Bifidobacterium, brown rot fungi,Geotrichum candidum, Mucor, Aspergillus oryzae, and Penicillium diversum. The preparation method includes: mixing well Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria,phosphorus-accumulating bacteria and Bifidobacterium, adding the mixture into clean soil, farming earthworms with the soil for 12-14 days, and isolating the earthworms and the soil to obtain earthwormcast containing the mixed bacteria; mixing the earthworm cast containing the mixed bacteria and other bacteria, and immobilizing to obtain the microbial agent.

The invention discloses an organic fertilizer fermentation inoculant and application. The inoculant is composed of a primary fermentation inoculant body and a secondary fermentation inoculant body. The primary fermentation inoculant body comprises bacillus subtilis, bacillus licheniformis, trichoderma viride, aspergillus oryzae, pseudomonas, trichoderma koningii, geotrichum candidum, long spiny shells, ascomycetes, thermomyces lanuginosus and thermobifida fusca. The secondary fermentation inoculant body comprises halophilic bacteria, alkalophilic bacteria, azotobacter chroococcum, bacillus megatherium, streptomyces griseus, bacillus thuringiensis, brevibacillus laterosporus and bacillus mucilaginosus. Time for fermenting organic fertilizer with cow dung as the raw material can be effectively shortened, and diseases and insect pests in the fertilizer can be eliminated. Meanwhile, alkalophilic bacteria and bacillus thuringiensis are added so that the fermentation inoculant can adapt to saline-alkali soil and can prevent diseases and insect pests of plants, and the prepared organic fertilizer can improve the soil structure, reduces the salt content and the pH value in soil, improves soil fertility and is particularly beneficial to improvement of saline-alkali soil.