23 results about "Geotrichum" patented technology

This invention belongs to the field of bioenzymes, specifically relating to lipase mutants, their encoding genes, expression plasmids, and applications. The lipase mutant is an amino acid sequence containing at least one mutation, either M81C or F329R, in the wild-type amino acid sequence shown in SEQ ID NO.1. This invention innovatively mutates Met at position 81 (M81C) and / or Phe at position 329 (F329R) of the Geotrichum candida Y162 lipase GCL protein sequence (wild-type amino acid sequence); this effectively improves the enzyme activity, stability, and other properties of the mutant.

The application discloses application of a CsERF017 gene or a protein coded by the same in regulation of resistance of navel orange fruits to sour rot, and belongs to the technical field of molecular biology and fruit and vegetable preservation. A nucleotide sequence of the CsERF017 gene is shown in SEQ ID NO. 2, and an amino acid sequence of the protein coded by the same is shown in SEQ ID NO. 3. The application finds through experiments that the CsERF017 gene is up-regulated after infection of a citrus sour rot pathogen Geotrichum candidum, and silencing or knocking out the CsERF017 gene can enhance the resistance of navel orange fruits to sour rot, and the specific performance is that, compared with a control, the transgenic fruits after inoculation of the G. candidum have reduced symptoms of sour rot, and both a disease index and a TA content are reduced. The application provides an important gene bank and new insights for breeding of citrus disease-resistant varieties and development of molecular markers, and is helpful to fundamentally reduce the dependence on chemical synthetic pesticides in postharvest preservation treatment of citrus fruits.

This invention belongs to the field of microbial technology, specifically relating to an Kodamaea strain HBF12 and its applications. The Kodamaea strain HBF12 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC M 20241462. Verification through examples shows that this strain has significant inhibitory effects on *Geotrichum citri-aurantii* and *Penicillium digitatum*, and exhibits good preventative effects against postharvest diseases of citrus, particularly sour rot and green mold. Strain HBF12 demonstrates good biocontrol potential, providing a new resource for the safe control of postharvest diseases of citrus.

The invention relates to a biofungicide based on the bacterium Pseudomonas lini strain S57, RGM 2930 CChRGM (Chilean Collection of Microbial Genetic Resources) for agricultural use, especially in saline-boric environments. The biofungicide can be developed as solid and liquid formulations. Where the strain S57, has fungicidal properties against the phytopathogenic fungi Botrytis cinerea. Fusarium oxysporum, Monilinia fructicola, Alternaria sp., Geotrichum candidum, and Macrophomina phaseolina, both in the presence and absence of saline-bone conditions.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

The application discloses application of Geotrichum candidum as a biomarker in heart failure diagnosis. The application studies the correlation between oral flora and heart failure, and finds through detection of clinical samples that the Geotrichum candidum can be used as an independent biomarker for diagnosing heart failure. Meanwhile, the application provides a biomarker combination comprising the Geotrichum candidum, and the biomarker combination has high heart failure diagnosis efficiency. The application can realize early diagnosis of heart failure, guide clinicians to provide a prevention or treatment scheme for a subject, and thus better intervene in the disease process of heart failure occurrence and development.

The invention provides a cotton straw feed and a preparation method thereof, and belongs to the technical field of feeds. The cotton straw feed provided by the invention is prepared from a cotton straw mixture and a zymophyte solution, the cotton straw mixture is composed of tomato peel, candy pomace and cotton straw, and the weight ratio of the tomato peel to the candy pomace to the cotton straw is (4-6): (2-4): (11-13); the zymophyte liquid comprises lactobacillus plantarum, candida utilis, saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, geotrichum candidum, bacillus licheniformis and pseudomonas putida. The tomato peel, the sugar residues and the cotton straw are scientifically matched, through the synergistic effect of components and taste, the palatability of the feed is remarkably improved, livestock are attracted to actively eat the feed, through superposition of multiple nutritional components, the content of key nutritional indexes such as protein and vitamins of the feed is effectively increased, nutritional advantage complementation of the raw materials is achieved, and the feed is suitable for large-scale production. The feed quality is comprehensively optimized.

This invention discloses bacterial strains, compound microbial agents, and a method for rapidly preparing Luocheng rice noodles, relating to the field of food processing technology. The bacterial strains used for preparing Luocheng rice noodles include *Geotrichum candida* (…). Galactomyces candidum ) Mei3303.1, Pediococcus acidilactici ( Pediococcus acidilactici Mei.1103.1 and Lactobacillus paracasei ( Lactobacillus paracasei Both Mei1104.02 and Mei1104.02 have been deposited at the China General Microbiological Culture Collection Center. The beneficial effects of this invention are that by strengthening the fermentation process with compound microbial agents, it can improve fermentation efficiency, shorten fermentation time, inhibit the growth of other miscellaneous bacteria, and improve the flavor stability and safety of rice noodles.

The invention belongs to the technical field of biological agents, and particularly relates to trichoderma harzianum, a biocontrol agent, a bio-organic fertilizer, a method for preventing and treating plant diseases and application. The invention provides a trichoderma harzianum P11 with the preservation number of CCTCC NO: M 2024648. The trichoderma harzianum P11 can improve the relative expression quantity of disease-resistant positive correlation genes PnCAD, PnSN1 and PnDEFL1 in plants and improve the activity of disease-resistant related enzyme activities SOD, POD and CAT, and the trichoderma harzianum P11 can damage cell membranes of pathogenic bacteria and inhibit the growth of the disease pathogenic bacteria. The trichoderma harzianum P11 has good rhizosphere colonization capability. The result of the embodiment shows that the trichoderma harzianum P11 can reduce the morbidity of panax notoginseng field root rot, and can also antagonize ginseng alternaria alternata, magnaporthe oryzae, polygonatum kingianum colletotrichum, geotrichum candidum, alternaria solani, cylindrosporium destroyed, phytophthora capsici and conidiospora spiraea.

The invention discloses aspergillus niger and application thereof. The preservation name of the aspergillus niger is Aspergillus niger JS-1, and the aspergillus niger is named as Aspergillus niger JS-1. The preservation unit is China Center for Type Culture Collection; the preservation address is Wuhan University, Wuhan, China; the preservation date of the strain is 2017.1. 11; and the preservation number of the strain is CCTCCM2017024. Experiments prove that the aspergillus niger has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various fruit and vegetable postharvest rot-causing fungi including penicillium digitatum, penicillium italicum, geotrichum candidum, botrytis cinerea, fusarium tripteroides and penicillium expansum. The compound can obviously influence the pathogenic activity of postharvest rot-causing fungi on fruits, is expected to be prepared into a microbial preparation for biological control of fruits, provides a new choice for developing a new fruit preservative, and has good economic and social benefits.

The invention discloses geotrichum candidum fermented coffee beans and a method for improving the quality of coffee by using the geotrichum candidum fermented coffee beans. According to the technical scheme, the Geotrichum candidum is adopted, the strain number of the Geotrichum candidum is CAU266, and the registration number of the Geotrichum candidum in the China Committee for Culture Collection of Microorganisms is CGMCC No. 30151. After the geotrichum candidum CAU266 is adopted for fermentation, the content of caffeine and the content of trigonelline which are bitter substances in the coffee are reduced by 58.5% and 37.1% respectively, and the bitter taste of the coffee is relieved; compared with natural fermentation, the content of 2, 3-butanediol in volatile substances is increased by 230%, so that the coffee has the flavor characteristic of Maotai-flavor liquor. The strain disclosed by the invention can be used as an excellent coffee bean leavening agent and is applied to coffee flavor improvement and quality improvement.

The invention discloses anti-geotrichum candidum lactic acid bacteria and a screening method and application thereof.The anti-geotrichum candidum lactic acid bacteria obtained through screening have the excellent anti-geotrichum candidum effect and can serve as natural, safe and efficient biological control bacteria to be applied to fruits and vegetables, growth of geotrichum candidum is inhibited, and microbial strain resources are provided for fruit and vegetable postharvest sour rot. Besides, lactic acid bacteria and / or lactic acid bacteria cell-free supernate with the effect of resisting geotrichum candidum are / is added into the prepared preservative film or nano-emulsion, the prepared preservative film or coating is applied to preservation of fruits and vegetables, sour rot of the picked fruits and vegetables can be reduced, embedding substances can be released in the storage period, the shelf life is prolonged, and the preservative film or coating is suitable for being used for preservation of fruits and vegetables. Meanwhile, the fruit and vegetable preservative has the functions of isolation protection, fruit and vegetable water loss relief, air conditioning, oxidation resistance and bacteria resistance, and is an effective strategy for reducing the influence of postharvest diseases of fruits and vegetables.

The invention provides a preparation method of a polypeptide product containing osteogenic growth peptide, which comprises the following steps: sterilizing bone marrow or blood of an animal as a raw material; after sterilization, adjusting the pH value to 6-8, inoculating 1-3% of micrococcus luteus (1-8 * 10 < 9 > cfu / mL), inoculating 2-5% of lactobacillus curvatus (1-8 * 10 < 10 > cfu / mL), inoculating 0.5-4% of bacillus coagulans (1-8 * 10 < 8 > cfu / g), and fermenting at 30-42 DEG C for 2-6 hours; adjusting the temperature to 20-30 DEG C and the pH value to 6-8, inoculating 1-8% of geotrichum candidum (1-8 * 10 < 6 > cfu / mL), fermenting for 1-5 days, and sterilizing for 20-40 minutes under the microwave of 200-500 W to obtain fermentation liquor; adjusting the pH value of the fermentation liquor to 5-9, adding 0.5-5% of kiwi fruit protease, bromelain and alkaline protease according to a ratio of 1: (1-4): (2-5), carrying out enzymolysis at 45-65 DEG C for 2-8 hours, heating to 85-95 DEG C, and carrying out heat preservation for 20-40 minutes to obtain enzymatic hydrolysate; and separating from the enzymatic hydrolysate to obtain the polypeptide product containing the osteogenic growth peptide. The invention provides a brand-new method for preparing the polypeptide product containing the osteogenic growth peptide, the process is relatively simple, and the yield of the osteogenic growth peptide is relatively high, so that the cost is reduced.

The invention discloses a tundra mucor strain and application thereof in a microbial insecticide, and relates to the technical field of tundra mucor strain and insecticide application, the tundra mucor strain is separated from naturally infected larch ascochis Djak, is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 11, 2025, and has the preservation number of CGMCC NO.9687. The invention also discloses a preparation method of the tundra mucor strain and application of the tundra mucor strain in a microbial insecticide, and the preparation method of the tundra mucor strain and application of the tundra mucor strain in the microbial insecticide. The preservation number is CGMCC (China General Microbiological Culture Collection Center According to the permafrost mucor strain and the application thereof in the microbial insecticide, the permafrost mucor strain MG is separated from the bodies of the larch Ya's larva inchworm which is naturally dead in the field, and the permafrost mucor strain MG is identified by using systematic morphology and molecular biology methods. Before, domestic and foreign literatures do not systematically report the pathogenicity and prevention and control potential of the permafrost mucor on the forest pests, and the research fills the blank of related research in the field of forestry pathogenic fungi.

The invention discloses streptomyces corchorusii and application thereof, the streptomyces corchorusii is separated from soil, the classification name of the streptomyces corchorusii is Streptomyces corchorusii Sc75, the streptomyces corchorusii is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.31399. The streptomyces corchorusii is a streptomyces corchorusii strain. The invention further provides a fermentation extracting solution of the strain and volatile antibacterial substances of the strain. The strain has a broad-spectrum antibacterial effect. The Sc75 strain and various bacteriostatic active substances generated by the Sc75 strain can be used for prevention and control of various diseases and have good prevention and control effects. The invention relates to the field of agricultural machinery, in particular to the field of agricultural machinery, such as rice blast caused by Magnaporthe oryzae, apple alternaria leaf spot caused by Alternaria alternata, gray mold on grapes and tomatoes caused by Botrytis cinerea, and sour rot of nectarines caused by Geotrichum candidum, as well as the field of agricultural machinery, such as rice blast caused by Magnaporthe oryzae, apple alternaria leaf spot caused by Alternaria alternata, gray mold on grapes and tomatoes caused by Botrytis cinerea, and sour rot of nectarines caused by Geotrichum candidum. The strain shows huge application potential in plant disease control and fruit preservation.

The invention provides cotton straw feed without leaves and a preparation method of the cotton straw feed, and belongs to the technical field of feed. The leaf-removed cotton straw feed provided by the invention is prepared from a leaf-removed cotton straw mixture and a zymophyte solution, and the leaf-removed cotton straw mixture is composed of mulched and planted corn straws, hay and leaf-removed cotton straws according to a weight ratio of (14-16): (29-31): (54-56). The zymophyte liquid comprises lactobacillus plantarum, candida utilis, saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, geotrichum candidum, bacillus licheniformis and pseudomonas putida. According to the method, the leaf-removed cotton straws and the high-humidity and high-acidity mulch-planted corn straws are proportionally mixed and fermented, so that the defects of strain activity inhibition, poor feed palatability and the like existing in single fermentation are overcome, the respective characteristics are fully exerted, and the overall efficiency of straw feed is further remarkably improved.

The invention discloses geotrichum candidum YSH with a function of repairing oxidative browning of grape juice and application of the geotrichum candidum YSH, and relates to the technical field of microorganisms. The classification name of the geotrichum candidum YSH is Geotrichum candidum, the preservation number of the geotrichum candidum YSH is GDMCC No: 66138, and the preservation date of the geotrichum candidum YSH is April 11, 2025. According to the method, the chromaticity of the oxidized and browned grape juice can be reduced, the appearance quality can be improved, the total polyphenol of the grape juice can be increased, and the antioxidant power index of the grape juice can be improved.

The invention belongs to the technical field of microorganisms, and relates to a high-sugar-resistant, aroma-producing and wine-producing geotrichum candidum strain and application thereof. The high-sugar-resistant, aroma-producing and wine-producing geotrichum candidum strain BS-2 is preserved in the China Center for Type Culture Collection on January 8, 2025, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 2025067. The invention further discloses application of the geotrichum candidum in production of acid, ester, alcohol and aldehyde ketone substances. The bacterial colony is flat, milky white and flock-shaped, and the cell is cylindrical. The strain has excellent salt (6%, g / L) and acid (pH 3.0) tolerance, good ethanol tolerance (4%, V / V), sugar tolerance (400g / L), high ester yield (7.11 g / L) and certain wine production capacity (2.9% vol). GC-MS (Gas Chromatography-Mass Spectrometer) analysis shows that the relative content of total ester is up to 33.31 The hemolysis effect is avoided, and the hereditary stability is good. Therefore, a theoretical basis is provided for unique flavor application research of the strain leavening agent in fermentation of foods, beverages, spices, cosmetics and the like.

The invention belongs to the field of biological enzymes, and particularly relates to a lipase mutant as well as a coding gene, an expression plasmid and application thereof, and the lipase mutant is an amino acid sequence with at least one mutation of M81C and F329R in a wild amino acid sequence as shown in SEQ ID NO.1. According to the invention, Met at the 81 site of a geotrichum candidum Y162 lipase GCL protein sequence (wild amino acid sequence) is innovatively mutated into Cys (M81C) and / or Phe at the 329 site of the geotrichum candidum Y162 lipase GCL protein sequence (wild amino acid sequence) is innovatively mutated into Arg (F329R); therefore, the enzyme activity, stability and other properties of the mutant can be effectively improved.

The present application relates to the field of natural flavonoid extraction, and more specifically, it relates to a composite plant fermentation extract and a preparation method thereof. A preparation method of a composite plant fermentation extract comprises the following steps: Step 1: crushing mulberry leaves, kudzu root, and cinnamon bark and mixing them with water to obtain a fermented material; Step 2: steaming the fermented material at 110-130°C for 25-40 minutes to obtain a steamed mixture; Step 3: cooling the steamed mixture to 25-30°C, adding Eurotium cristatum, Lactobacillus acidophilus, and Geotrichum candidum to the steamed mixture, stirring and mixing, soaking and fermenting for 5-10 days to obtain a fermentation mixture; Step 4: sterilizing and centrifuging the fermentation mixture, collecting the supernatant, and obtaining a composite plant fermentation extract. The present application has the advantages of increasing the flavonoid extraction content and enhancing the antioxidant effect.

The invention discloses application of a luteolin-zinc composite material in multi-target inhibition of valsa mali, and the luteolin-zinc composite material can cause the membrane potential change of a hypha cell membrane of the valsa mali to generate depolarization and destroy the integrity of the cell membrane to cause the leakage of intracellular nucleic acid and protein, so that the valsa mali is inhibited. An inhibiting effect and a killing effect are achieved on apple rot fungi; the ATP level in cells of valsa mali can be remarkably reduced, the activity of alkaline phosphatase is improved, and the content of malondialdehyde and the level of active oxygen are improved, so that bacteriostasis is realized through multiple ways of interfering energy metabolism, inducing membrane lipid peroxidation, oxidative stress and the like; in drug research and development and practical application, people can more scientifically and efficiently use the composite material to resist the apple rot fungi through the clear key concentration value, namely, the minimum inhibitory concentration of 4mM, and the composite material has important significance for guaranteeing healthy development of the apple industry and is expected to bring a new solution to prevention and treatment of the apple rot.

The invention discloses a microorganism bacterium brick for promoting plant growth and a preparation method and application thereof, the microorganism bacterium brick is composed of vinasse obtained after compound microorganism fermentation, coco coir and a binder, and the compound microorganism bacterium brick comprises 30 wt%-35 wt% of bacillus subtilis, 30 wt%-35 wt% of geotrichum candidum and 30 wt%-35 wt% of aspergillus niger. After fermentation with the compound microbial agent, the content of nutrient elements such as nitrogen, phosphorus and potassium in the vinasse is obviously increased, and the content of crude fibers is reduced. The invention further discloses a production process for preparing the microbial bacterial brick from the vinasse fermented by the compound microbial bacterial agent and application of the microbial bacterial brick to plant cultivation. The prepared microbial bacterial brick can effectively promote the growth of ryegrass. The method not only solves the problem of disposal of waste materials after wine brewing and recycles the waste materials, but also can be widely applied to the fields of agriculture, horticulture, ecological restoration and the like, and has a wide market prospect.

The invention belongs to the technical field of microbial engineering, and particularly relates to a composite fermentation inoculant for increasing the relative content of ethyl caprylate and application thereof.The composite fermentation inoculant is composed of saccharomyces cerevisiae and geotrichum galactose yeast, and the composite fermentation inoculant is applied to fruit wine production. Compared with a method only adopting the saccharomyces cerevisiae culture solution and the geotrichum galactose yeast culture solution, the relative content of ethyl caprylate in the fruit wine and the richness of ester components in the fruit wine are greatly improved. Specifically, by adopting the method disclosed by the invention, the ester content and substance composition in the grape, pitaya and passion fruit wine can be improved and are remarkably improved compared with those in a fruit juice natural fermentation group, and the produced fruit wine remarkably shows brandy flavor, similar to pineapple flavor and other flavors.