43 results about "Geotrichum" patented technology

The invention discloses a compound bacterial fertilizer for reducing soil obstacles as well as a preparation method and application thereof, and relates to the technical field of biology. The compound bacterial fertilizer is prepared from a mortierella indurata bacterial agent, a trichoderma hedgehog bacterial agent, an osmanthus fragrans geotrichum bacterial agent and a bacillus cereus bacterial agent, the mortierella cinerea preparation is obtained by uniformly mixing a mortierella cinerea spore suspension and corn straw powder and then drying; the trichoderma hedgehog fungicide is obtained by uniformly mixing a trichoderma hedgehog spore suspension and corn straw powder and then drying; the osmanthus fragrans geotrichum fungicide is obtained by uniformly mixing osmanthus fragrans geotrichum spore suspension and corn straw powder and then drying, the bacillus cereus microbial inoculum is obtained by freeze-drying a bacillus cereus fermentation thallus. By applying the compound bacterial fertilizer to the surface layer of ginseng continuous cropping soil, the soil condition of the ginseng continuous cropping soil can be improved, ginseng growth is promoted, ginseng root rot is reduced, and the survival rate of ginseng plants is increased.

The invention discloses a yeast hydrolysate as well as a preparation method and application thereof. The method comprises the following steps: preparing a saccharomyces cerevisiae HKB-145051 zymophyte solution and a geotrichum candidum zymophyte solution; inoculating the saccharomyces cerevisiae HKB-145051 zymophyte liquid to a brewer's grain fermentation medium for anaerobic fermentation to obtain saccharomyces cerevisiae zymophyte milk; inoculating the saccharomyces cerevisiae fermented bacterial milk with the geotrichum candidum fermented bacterial liquid, and carrying out aerobic fermentation to obtain bacterial milk; and adding a wall breaking agent into the bacterial milk to carry out wall breaking treatment, and then drying and crushing to obtain the yeast hydrolysate. The yeast hydrolysate provided by the invention is rich in protein content, low in flavoring substance content and relatively low in production cost, can replace part of soybean meal to be applied to livestock and poultry breeding, and has important practical significance in promoting cost reduction and efficiency improvement of the livestock and poultry breeding industry.

The invention discloses geotrichum candidum and application thereof in degradation of a polyethylene plastic mulching film, and relates to the technical field of microorganisms and application thereof. The strain number of the Geotrichum candidum is MY-0603, the Geotrichum candidum is preserved in the China Center for Type Culture Collection, and the preservation number of the Geotrichum candidum is CCTCC (China Center for Type Culture Collection) NO: M2025455. Experimental results show that the screened strain shows excellent performance in the aspect of biodegradation of polyethylene plastic, and after 21 days of biodegradation treatment, the weight loss rate of the polyethylene plastic mulching film reaches 8%. The method has the advantages of being environmentally friendly, low in cost, easy and convenient to operate and the like, and the obtained strain has the efficient degradation characteristic. Therefore, the invention provides a new strain resource and thought for bioremediation of polyethylene waste in the environment, and has a wide application prospect.

This invention belongs to the field of bioenzymes, specifically relating to lipase mutants, their encoding genes, expression plasmids, and applications. The lipase mutant is an amino acid sequence containing at least one mutation, either M81C or F329R, in the wild-type amino acid sequence shown in SEQ ID NO.1. This invention innovatively mutates Met at position 81 (M81C) and / or Phe at position 329 (F329R) of the Geotrichum candida Y162 lipase GCL protein sequence (wild-type amino acid sequence); this effectively improves the enzyme activity, stability, and other properties of the mutant.

The application discloses application of a CsERF017 gene or a protein coded by the same in regulation of resistance of navel orange fruits to sour rot, and belongs to the technical field of molecular biology and fruit and vegetable preservation. A nucleotide sequence of the CsERF017 gene is shown in SEQ ID NO. 2, and an amino acid sequence of the protein coded by the same is shown in SEQ ID NO. 3. The application finds through experiments that the CsERF017 gene is up-regulated after infection of a citrus sour rot pathogen Geotrichum candidum, and silencing or knocking out the CsERF017 gene can enhance the resistance of navel orange fruits to sour rot, and the specific performance is that, compared with a control, the transgenic fruits after inoculation of the G. candidum have reduced symptoms of sour rot, and both a disease index and a TA content are reduced. The application provides an important gene bank and new insights for breeding of citrus disease-resistant varieties and development of molecular markers, and is helpful to fundamentally reduce the dependence on chemical synthetic pesticides in postharvest preservation treatment of citrus fruits.

The invention relates to a method for preparing a Ginkgo biloba leaf extract highly concentrated in proanthocyanidins, and practically free of flavonoids and terpene trilactones. The invention also relates to the use of cheese fungal strains such as Penicillium Roquefortii PA and Geotrichum Candidum Geo 15, to catabolize high molecular weight proanthocyanidin polymers into small molecules in order to have beneficial effects on health.

This invention belongs to the field of microbial technology, specifically relating to an Kodamaea strain HBF12 and its applications. The Kodamaea strain HBF12 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC M 20241462. Verification through examples shows that this strain has significant inhibitory effects on *Geotrichum citri-aurantii* and *Penicillium digitatum*, and exhibits good preventative effects against postharvest diseases of citrus, particularly sour rot and green mold. Strain HBF12 demonstrates good biocontrol potential, providing a new resource for the safe control of postharvest diseases of citrus.

The invention relates to the technical field of biological fermentation. The invention provides a strain of Geotrichum candidum Do-Ya-2-12, and the preservation number of the strain of Geotrichum candidum Do-Ya-2-12 is CGMCC (China General Microbiological Culture Collection Center) No. 33104. The strain of the geotrichum candidum Do-Ya-2-12 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the strain is CGMCC No.33104, the preservation time of the strain is December 17, 2024, the preservation center is called CGMCC for short, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain provided by the invention can replace a conventionally used carbon source and nitrogen source with a corn soaking solution or corn steep liquor, is environment-friendly, improves the conversion rate of lactic acid by microorganisms such as yeast, reduces the production cost, simplifies the production process, and realizes efficient output of single-cell protein.

The invention provides a cheese product which comprises the following components in percentage by mass: 5-7% of white granulated sugar; 0.002% to 0.005% of lactococcus lactis diacetyl subspecies and leuconostoc mesenteroides; 0.01 to 0.02 percent of geotrichum candidum; 0-0.3% of a stabilizer; the rest component is raw milk; wherein the ratio of the lactococcus lactis diacetyl subspecies to the leuconostoc mesenteroides is 1: 1; the invention also provides a preparation method of the cheese product. According to the invention, the cheese fermented by unconventional beneficial strains is produced, and fermented dairy products with different flavors and effects are brought by different leavening agents and different production processes.

The invention relates to a biofungicide based on the bacterium Pseudomonas lini strain S57, RGM 2930 CChRGM (Chilean Collection of Microbial Genetic Resources) for agricultural use, especially in saline-boric environments. The biofungicide can be developed as solid and liquid formulations. Where the strain S57, has fungicidal properties against the phytopathogenic fungi Botrytis cinerea. Fusarium oxysporum, Monilinia fructicola, Alternaria sp., Geotrichum candidum, and Macrophomina phaseolina, both in the presence and absence of saline-bone conditions.

The present invention belongs to the field of microorganisms, and specifically discloses an Aspergillus fusca F25 strain capable of degrading lignin and cellulose at low temperature and an application thereof; the Aspergillus fusca F25 strain is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; the deposit date is January 25, 2024, and the deposit number is CGMCC No. 41052; the Aspergillus fusca F25 strain has the ability to degrade lignin and cellulose at low temperature, and can be used for the degradation of agricultural and forestry wastes at low temperature. Furthermore, the present invention also finds that the Aspergillus fusca F25 strain is combined with Geotrichum candidum and Acinetobacter to form a composite bacterial agent, which can produce a synergistic effect and produce a better effect of degrading agricultural and forestry wastes.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

The present invention discloses the application of oral microbiota in the diagnosis of heart failure. The present invention has for the first time discovered a combination of biomarkers related to the diagnosis of heart failure, namely Streptococcus, Neisseria, Neisseria subflava, Veillonella parvula, Neisseria cinerea, Haemophilus parainfluenzae, Neisseria meningitidis, Geotrichum candidum, Haemophilus influenzae, and Veillonella massiliensis. By detecting the abundances of the biomarkers in the oral microbiota of a subject, heart failure can be diagnosed, thereby better intervening in the occurrence and development of heart failure and guiding clinicians to provide prevention or treatment plans for the subject.

The application discloses application of Geotrichum candidum as a biomarker in heart failure diagnosis. The application studies the correlation between oral flora and heart failure, and finds through detection of clinical samples that the Geotrichum candidum can be used as an independent biomarker for diagnosing heart failure. Meanwhile, the application provides a biomarker combination comprising the Geotrichum candidum, and the biomarker combination has high heart failure diagnosis efficiency. The application can realize early diagnosis of heart failure, guide clinicians to provide a prevention or treatment scheme for a subject, and thus better intervene in the disease process of heart failure occurrence and development.

The invention provides a cotton straw feed and a preparation method thereof, and belongs to the technical field of feeds. The cotton straw feed provided by the invention is prepared from a cotton straw mixture and a zymophyte solution, the cotton straw mixture is composed of tomato peel, candy pomace and cotton straw, and the weight ratio of the tomato peel to the candy pomace to the cotton straw is (4-6): (2-4): (11-13); the zymophyte liquid comprises lactobacillus plantarum, candida utilis, saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, geotrichum candidum, bacillus licheniformis and pseudomonas putida. The tomato peel, the sugar residues and the cotton straw are scientifically matched, through the synergistic effect of components and taste, the palatability of the feed is remarkably improved, livestock are attracted to actively eat the feed, through superposition of multiple nutritional components, the content of key nutritional indexes such as protein and vitamins of the feed is effectively increased, nutritional advantage complementation of the raw materials is achieved, and the feed is suitable for large-scale production. The feed quality is comprehensively optimized.

This invention discloses bacterial strains, compound microbial agents, and a method for rapidly preparing Luocheng rice noodles, relating to the field of food processing technology. The bacterial strains used for preparing Luocheng rice noodles include *Geotrichum candida* (…). Galactomyces candidum ) Mei3303.1, Pediococcus acidilactici ( Pediococcus acidilactici Mei.1103.1 and Lactobacillus paracasei ( Lactobacillus paracasei Both Mei1104.02 and Mei1104.02 have been deposited at the China General Microbiological Culture Collection Center. The beneficial effects of this invention are that by strengthening the fermentation process with compound microbial agents, it can improve fermentation efficiency, shorten fermentation time, inhibit the growth of other miscellaneous bacteria, and improve the flavor stability and safety of rice noodles.

The bacillus albus MY-7 disclosed by the invention has a gene related to synthesis of bacteriocin, has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various plant pathogenic bacteria including cabbage alternaria alternata, fusarium tripteroides, alternaria alternata, geotrichum candidum, colletotrichum gloeosporioides, broad bean botrytis cinerea, penicillium digitatum and A. arundinis; and the bacillus subtilis has good environmental adaptability, is expected to be used for biological prevention and control and preparation of biological prevention and control agents, biological fertilizers, plant growth promoters and related microbial preparations, and is favorable for reducing the use of chemical pesticides and promoting the development of sustainable agriculture after being popularized.

The invention belongs to the technical field of biological agents, and particularly relates to trichoderma harzianum, a biocontrol agent, a bio-organic fertilizer, a method for preventing and treating plant diseases and application. The invention provides a trichoderma harzianum P11 with the preservation number of CCTCC NO: M 2024648. The trichoderma harzianum P11 can improve the relative expression quantity of disease-resistant positive correlation genes PnCAD, PnSN1 and PnDEFL1 in plants and improve the activity of disease-resistant related enzyme activities SOD, POD and CAT, and the trichoderma harzianum P11 can damage cell membranes of pathogenic bacteria and inhibit the growth of the disease pathogenic bacteria. The trichoderma harzianum P11 has good rhizosphere colonization capability. The result of the embodiment shows that the trichoderma harzianum P11 can reduce the morbidity of panax notoginseng field root rot, and can also antagonize ginseng alternaria alternata, magnaporthe oryzae, polygonatum kingianum colletotrichum, geotrichum candidum, alternaria solani, cylindrosporium destroyed, phytophthora capsici and conidiospora spiraea.

The invention belongs to the technical field of microorganisms, and relates to a desertification remediation fungicide and application thereof. The invention provides a desertification remediation microbial agent which is prepared by the following steps: respectively fermenting bacillus subtilis, geotrichum candidum and candida utilis to obtain bacterial liquids, and mixing the bacterial liquids. The bacillus subtilis, the geotrichum candidum and the candida utilis are synergistically matched, and when the bacillus subtilis, the geotrichum candidum and the candida utilis are applied to the desertification soil remediation process, the compressive strength and the shear strength of soil can be effectively improved, and the dosage of urea and calcium superphosphate is reduced.

The invention discloses aspergillus niger and application thereof. The preservation name of the aspergillus niger is Aspergillus niger JS-1, and the aspergillus niger is named as Aspergillus niger JS-1. The preservation unit is China Center for Type Culture Collection; the preservation address is Wuhan University, Wuhan, China; the preservation date of the strain is 2017.1. 11; and the preservation number of the strain is CCTCCM2017024. Experiments prove that the aspergillus niger has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various fruit and vegetable postharvest rot-causing fungi including penicillium digitatum, penicillium italicum, geotrichum candidum, botrytis cinerea, fusarium tripteroides and penicillium expansum. The compound can obviously influence the pathogenic activity of postharvest rot-causing fungi on fruits, is expected to be prepared into a microbial preparation for biological control of fruits, provides a new choice for developing a new fruit preservative, and has good economic and social benefits.

The invention discloses geotrichum candidum fermented coffee beans and a method for improving the quality of coffee by using the geotrichum candidum fermented coffee beans. According to the technical scheme, the Geotrichum candidum is adopted, the strain number of the Geotrichum candidum is CAU266, and the registration number of the Geotrichum candidum in the China Committee for Culture Collection of Microorganisms is CGMCC No. 30151. After the geotrichum candidum CAU266 is adopted for fermentation, the content of caffeine and the content of trigonelline which are bitter substances in the coffee are reduced by 58.5% and 37.1% respectively, and the bitter taste of the coffee is relieved; compared with natural fermentation, the content of 2, 3-butanediol in volatile substances is increased by 230%, so that the coffee has the flavor characteristic of Maotai-flavor liquor. The strain disclosed by the invention can be used as an excellent coffee bean leavening agent and is applied to coffee flavor improvement and quality improvement.

The invention discloses application of geotrichum candidum as a biomarker in diagnosis of heart failure. The correlation between oral flora and heart failure is studied, and clinical sample detection shows that geotrichum candidum can be used as an independent biomarker for diagnosing heart failure. Meanwhile, the invention provides a biomarker combination containing geotrichum candidum, and the biomarker combination has relatively high heart failure diagnosis efficiency. According to the invention, early diagnosis of heart failure can be realized, and a clinician is guided to provide a prevention or treatment scheme for a subject, so that the disease progress of occurrence and development of heart failure can be better intervened.

The invention discloses anti-geotrichum candidum lactic acid bacteria and a screening method and application thereof.The anti-geotrichum candidum lactic acid bacteria obtained through screening have the excellent anti-geotrichum candidum effect and can serve as natural, safe and efficient biological control bacteria to be applied to fruits and vegetables, growth of geotrichum candidum is inhibited, and microbial strain resources are provided for fruit and vegetable postharvest sour rot. Besides, lactic acid bacteria and / or lactic acid bacteria cell-free supernate with the effect of resisting geotrichum candidum are / is added into the prepared preservative film or nano-emulsion, the prepared preservative film or coating is applied to preservation of fruits and vegetables, sour rot of the picked fruits and vegetables can be reduced, embedding substances can be released in the storage period, the shelf life is prolonged, and the preservative film or coating is suitable for being used for preservation of fruits and vegetables. Meanwhile, the fruit and vegetable preservative has the functions of isolation protection, fruit and vegetable water loss relief, air conditioning, oxidation resistance and bacteria resistance, and is an effective strategy for reducing the influence of postharvest diseases of fruits and vegetables.

The invention relates to the field of natural flavone extraction, in particular to a composite plant fermentation extract and a preparation method thereof. The preparation method of the compound plant fermentation extract comprises the following steps: step 1, crushing mulberry leaves, pachyrhizua angulatus and cinnamon, and mixing with water to obtain a to-be-fermented material; step 2, cooking the to-be-fermented material for 25 to 40 minutes under the condition of 110 to 130 DEG C, so as to obtain a cooked mixture; step 3, cooling the cooked mixture to 25-30 DEG C, adding eurotium cristatum, lactobacillus acidophilus and geotrichum candidum into the cooked mixture, stirring and mixing, and soaking and fermenting for 5-10 days to obtain a fermented mixture; and step 4, sterilizing and centrifuging the fermentation mixture, and collecting supernate to obtain the composite plant fermentation extract. The method has the advantages of increasing the flavone extraction content, enhancing the antioxidant effect and the like.

The invention provides a preparation method of a polypeptide product containing osteogenic growth peptide, which comprises the following steps: sterilizing bone marrow or blood of an animal as a raw material; after sterilization, adjusting the pH value to 6-8, inoculating 1-3% of micrococcus luteus (1-8 * 10 < 9 > cfu / mL), inoculating 2-5% of lactobacillus curvatus (1-8 * 10 < 10 > cfu / mL), inoculating 0.5-4% of bacillus coagulans (1-8 * 10 < 8 > cfu / g), and fermenting at 30-42 DEG C for 2-6 hours; adjusting the temperature to 20-30 DEG C and the pH value to 6-8, inoculating 1-8% of geotrichum candidum (1-8 * 10 < 6 > cfu / mL), fermenting for 1-5 days, and sterilizing for 20-40 minutes under the microwave of 200-500 W to obtain fermentation liquor; adjusting the pH value of the fermentation liquor to 5-9, adding 0.5-5% of kiwi fruit protease, bromelain and alkaline protease according to a ratio of 1: (1-4): (2-5), carrying out enzymolysis at 45-65 DEG C for 2-8 hours, heating to 85-95 DEG C, and carrying out heat preservation for 20-40 minutes to obtain enzymatic hydrolysate; and separating from the enzymatic hydrolysate to obtain the polypeptide product containing the osteogenic growth peptide. The invention provides a brand-new method for preparing the polypeptide product containing the osteogenic growth peptide, the process is relatively simple, and the yield of the osteogenic growth peptide is relatively high, so that the cost is reduced.

The invention discloses a tundra mucor strain and application thereof in a microbial insecticide, and relates to the technical field of tundra mucor strain and insecticide application, the tundra mucor strain is separated from naturally infected larch ascochis Djak, is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 11, 2025, and has the preservation number of CGMCC NO.9687. The invention also discloses a preparation method of the tundra mucor strain and application of the tundra mucor strain in a microbial insecticide, and the preparation method of the tundra mucor strain and application of the tundra mucor strain in the microbial insecticide. The preservation number is CGMCC (China General Microbiological Culture Collection Center According to the permafrost mucor strain and the application thereof in the microbial insecticide, the permafrost mucor strain MG is separated from the bodies of the larch Ya's larva inchworm which is naturally dead in the field, and the permafrost mucor strain MG is identified by using systematic morphology and molecular biology methods. Before, domestic and foreign literatures do not systematically report the pathogenicity and prevention and control potential of the permafrost mucor on the forest pests, and the research fills the blank of related research in the field of forestry pathogenic fungi.

The invention discloses streptomyces corchorusii and application thereof, the streptomyces corchorusii is separated from soil, the classification name of the streptomyces corchorusii is Streptomyces corchorusii Sc75, the streptomyces corchorusii is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.31399. The streptomyces corchorusii is a streptomyces corchorusii strain. The invention further provides a fermentation extracting solution of the strain and volatile antibacterial substances of the strain. The strain has a broad-spectrum antibacterial effect. The Sc75 strain and various bacteriostatic active substances generated by the Sc75 strain can be used for prevention and control of various diseases and have good prevention and control effects. The invention relates to the field of agricultural machinery, in particular to the field of agricultural machinery, such as rice blast caused by Magnaporthe oryzae, apple alternaria leaf spot caused by Alternaria alternata, gray mold on grapes and tomatoes caused by Botrytis cinerea, and sour rot of nectarines caused by Geotrichum candidum, as well as the field of agricultural machinery, such as rice blast caused by Magnaporthe oryzae, apple alternaria leaf spot caused by Alternaria alternata, gray mold on grapes and tomatoes caused by Botrytis cinerea, and sour rot of nectarines caused by Geotrichum candidum. The strain shows huge application potential in plant disease control and fruit preservation.

The invention provides cotton straw feed without leaves and a preparation method of the cotton straw feed, and belongs to the technical field of feed. The leaf-removed cotton straw feed provided by the invention is prepared from a leaf-removed cotton straw mixture and a zymophyte solution, and the leaf-removed cotton straw mixture is composed of mulched and planted corn straws, hay and leaf-removed cotton straws according to a weight ratio of (14-16): (29-31): (54-56). The zymophyte liquid comprises lactobacillus plantarum, candida utilis, saccharomyces cerevisiae, candida tropicalis, bacillus subtilis, geotrichum candidum, bacillus licheniformis and pseudomonas putida. According to the method, the leaf-removed cotton straws and the high-humidity and high-acidity mulch-planted corn straws are proportionally mixed and fermented, so that the defects of strain activity inhibition, poor feed palatability and the like existing in single fermentation are overcome, the respective characteristics are fully exerted, and the overall efficiency of straw feed is further remarkably improved.

The invention discloses a geotrichum candidum SX-Y3 (Geotrichum candidum SX-Y3) strain with aroma producing capacity and a preparation method of a fermented bean cereal beverage of the geotrichum candidum SX-Y3 strain. The aroma-producing geotrichum candidum strain is named as geotrichum candidum SX-Y3, the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO: 30636, and the strain has better growth environment adaptability and fermentation characteristic. The preparation method comprises the following steps: respectively pulping soybeans and millet, filtering to obtain soybean milk and millet slurry, sterilizing, compounding in proportion, adding glucose and lactic acid, blending, inoculating a geotrichum candidum SX-Y3 seed solution, fermenting for 30 hours, adding a stabilizer, homogenizing, filtering, and pasteurizing to obtain the fermented cereal beverage. The finished product is rich in fruit flavor, soft and fine in taste and rich in nutrition, and does not need to add any essence for seasoning. The strain provided by the invention can also be used for brewing various fruit and vegetable juice fermented drinks and low-alcohol fruit wine, and the fragrance of fermented flowers and fruits is increased without adding other essence substances.

The invention discloses an aquatic feed fermentation method of white spirit vinasse, relates to the technical field of white spirit vinasse, and solves the problems of poor feed palatability and low utilization rate caused by anti-nutritional ingredients such as high-content crude fibers contained in vinasse, and the method comprises the following steps: step 1, inoculating 0.1 g of bacillus subtilis powder into an LB liquid culture medium, putting into a shaking table with the temperature of 37 DEG C, and activating and culturing for 24 hours at the rotating speed of 150r / min to obtain a liquid seed A; 2, 0.1 g of candida utilis yeast powder is inoculated into a YPD liquid culture medium, the YPD liquid culture medium is placed in a shaking table at the temperature of 30 DEG C, activation culture is conducted for 24 h at the rotating speed of 150 r / min, and a liquid seed B is obtained; 3, inoculating the PDA liquid culture medium with the geotrichum candidum freeze-dried powder, placing the PDA liquid culture medium in a shaking table at the temperature of 30 DEG C, and performing constant-temperature culture at the rotating speed of 150r / min for 24 hours for activation. According to the preparation method for fermenting the white spirit vinasse by using the compound bacteria at the optimal temperature, the nutritional value of the vinasse is improved, and the feasibility of evaluating the fermented vinasse as a plant protein source substitute such as fish meal is realized.