44 results about "Oral Microflora" patented technology

The invention belongs to the field of biological medicine, and discloses an oral flora marker related to tongue squamous cell carcinoma (TSCC) and application of the oral flora marker. The oral cavity flora marker is Stomatobacterium longum (SBL), and the oral cavity flora marker is SBL (Stomatobacterium longum); the invention further discloses a diagnostic product of the TSCC, application of the SBL in preparation of a medicine for treating the TSCC and the medicine for treating the TSCC. The diagnosis product prepared by taking the SBL as the marker can quickly and effectively carry out early diagnosis on the TSCC so as to achieve the purposes of early discovery, early treatment and improvement of the survival rate. The medicine provided by the invention can specifically inhibit the SBL from secreting the nano extracellular vesicles so as to inhibit the expression of BRCA1 and further inhibit the expression of EXO1 and TP53BP1, so that the proliferation and cell cycle progress of TSCC cells are inhibited, and the treatment on the TSCC is realized. The medicine has good specificity and can be used for effectively treating TSCC.

The present invention relates to the technical field of oral medicine and oral medical products, and in particular to a targeted nano-enzyme antibacterial agent having an oral microbiota level adaptive regulation function and a preparation method therefor. Specifically, a nitrogen-doped zeolite imidazolate framework material is used as a carrier; a bimetallic monatomic nano-enzyme is prepared by means of a pyrolysis method; and an antibacterial peptide is used as a surface anchoring body and is modified on the surface of the nano-enzyme, so that a multifunctional nano-enzyme having a high targeting colonization ability, a high-efficiency antibacterial biofilm and an antibacterial effect is obtained. Also provided are a pharmaceutical composition, an antibacterial agent, and an oral care product containing the nano-enzyme.

The invention discloses an oral microbial marker composition and application thereof in preparation of a kit for diagnosing high altitude headache, and particularly relates to the technical field of molecular biotechnology detection. A diagnosis model constructed on the basis of oral flora finds that the provided oral flora marker combination can specifically recognize patients with high altitude headache from plateau healthy people. The kit is used for predicting the high-altitude headache illness condition and clinical auxiliary diagnosis, is good in detection specificity and high in sensitivity, can indicate the condition of the high-altitude headache tissue microbial flora, and can guide the clinical diagnosis mode of the high-altitude headache and the adjustment of medication so as to reduce the probability of misdiagnosis and missed diagnosis in clinical diagnosis. The oral flora marker has important application in the aspects of screening and diagnosis of high altitude headache and the like, so that health damage and life risks caused by diseases are reduced.

The application discloses a kind of partition delivery environment response type hydrogel microneedle and its preparation method and application in oral squamous carcinoma treatment.The hydrogel microneedle includes backing layer and the needle tip layer being arranged on the surface of the backing layer, the needle tip layer includes multiple array distribution microneedle tip body, each the needle tip body is connected with the backing layer respectively;Wherein, the backing layer has enzyme response, and the needle tip layer has acidic pH response.The microneedle uses innovative "integration partition design", and two hydrogels with different functions are integrated in single microneedle array: backing layer responds oral flora imbalance microenvironment, needle tip layer responds tumor weak acidic microenvironment.The design can realize partition, accurate, intelligentized synergistic treatment to oral mucosa surface and deep tumor tissue, so as to overcome the technical bottleneck of traditional dosage form in space selectivity, single drug type, release behavior uncontrollable and great systemic toxicity etc.

The invention relates to a dental and / or oral care product comprising between 0.0039% and 0.0078% by weight of nisin establishing and / or maintaining a pH of at least 4.5 in situ. Although said rather low nisin concentration does not provide an anti-bacterial effect, it is surprisingly and advantageously capable of maintaining stable pH values of at least 4.5, thereby preventing destruction of a subject's teeth enamel based on enamel demineralization effects at pH below 4.5, and said low nisin concentration advantageously further avoids dysbacteriosis, i.e. the oral microflora remains balanced, thus not leading to an increase of e.g. periodontitis associated bacteria.

The present invention pertains to a solid food product capable of effectively improving the oral flora in an oral cavity while using a plant-derived powder.　Specifically, the present invention pertains to a solid food product comprising the following components (A) and (B): (A) a plant-derived powder containing a nitrate salt; and (B) one or more salt compounds selected from organic acid salts (B1) and carbonate compounds (B2).

The present application relates to the technical field of biological detection and disease risk prediction, in particular to a periodontal disease risk prediction system based on cardiovascular and oral flora data fusion, comprising a multi-modal data acquisition module, a heterogeneous data preprocessing module, a cross-modal feature fusion module, a double-branch risk prediction model module and a risk grading output module. The multi-modal data acquisition module non-invasively acquires cardiovascular physiological data and oral mucosa swab flora sequencing data of the subject; the preprocessing module denoises and normalizes the time series physiological data, and completes species annotation and abundance correction of the flora data; the cross-modal feature fusion module realizes adaptive alignment and deep fusion of the two types of heterogeneous data through a cross-modal attention mechanism; the double-branch prediction model outputs a risk probability after being trained by a comorbidity correlation data set; and the risk grading module divides the individual risk level in combination with a dynamic threshold. The whole-body cardiovascular and oral flora data are deeply fused to improve the prediction accuracy.

The invention discloses an ionic liquid anti-caries coating with dual functions of bacteriostasis and mineralization promotion and a preparation method thereof, belongs to the technical field of dental caries prevention in stomatology, and aims to overcome the defects that an existing anti-caries material fluoride is insufficient in bacteriostasis effect and relatively short in residence time on a tooth surface. And the risk of oral flora imbalance caused by the generation of drug resistance possibly caused by antibacterial mouthwash is also caused. According to the invention, alkyl imidazole is taken as a main body, is paired with ions such as strontium and fluorine with a mineralization promoting function, is modified by a silane coupling agent, and is properly added with an excipient, so that the constructed ionic liquid coating can be adhered to the surfaces of teeth for a long time, the dual effects of bacteriostasis and mineralization promoting can be durably exerted, and a more excellent anti-caries effect can be realized.

The invention discloses an oral care composition for preventing and treating periodontitis, the oral care composition comprises a compound shown in a formula (I) and oral auxiliary materials, by controlling the concentration or dosage of a component (a) in the oral care composition, the oral care composition can generate the effective drug concentration in the range of 1.35-13mM in the oral cavity after being applied to the oral cavity, and the oral care composition can be used for preventing and treating periodontitis. According to the invention, porphyromonas gingivalis can be more effectively inhibited, and meanwhile, a beneficial bacterium lactobacillus reuteri and the total amount of flora in oral cavities of healthy people are not substantially influenced, so that the oral flora microenvironment and flora imbalance can be effectively improved, and oral related diseases can be further avoided or prevented.

The invention belongs to the field of pancreatic duct adenocarcinoma prediction, and provides a pancreatic duct adenocarcinoma prediction method and system based on oral flora. The pancreatic ductal adenocarcinoma prediction method based on oral flora comprises the following steps: acquiring relative abundance information of each biomarker in a potential biomarker set for diagnosing pancreatic ductal adenocarcinoma of a to-be-detected person; using the relative abundance information of each biomarker in the biomarker set and a pre-trained multivariate statistical model to obtain a probability value of suffering from pancreatic ductal adenocarcinoma; the method comprises the following steps: performing microbiome difference analysis on saliva, duodenal juice and pancreatic tissue samples of a pancreatic ductal adenocarcinoma patient group and a pancreatic benign disease patient group, and taking an intersection of differential bacteria in the samples to obtain a potential biomarker set for diagnosing the pancreatic ductal adenocarcinoma.

The invention discloses a non-invasive colorectal cancer early screening and diagnosis method and electronic equipment. The method comprises the following steps: collecting excrement, saliva, a blood sample and a standardized tongue image of a subject; species abundance information of intestinal flora and oral flora is obtained through metagenome sequencing, and the tumor marker level is obtained through blood detection; and inputting the multi-modal data into a pre-trained colorectal cancer risk prediction model, and outputting a risk probability. The model is obtained by training based on a machine learning algorithm by adopting a training data set containing healthy persons and patients, the architecture of the model adopts a late fusion strategy, clinical, flora and tongue picture features are respectively processed through three sub-models of logistic regression, a gradient boosting decision tree and a convolutional neural network, dynamic fusion is performed by utilizing a Self-Attention mechanism, and finally a diagnosis result is output. According to the method, through multi-modal information fusion, the screening accuracy, the standardization degree and the patient compliance are remarkably improved.

The application discloses an oral care composition and use, which comprises a compound shown in formula (I) and an oral auxiliary material, and by controlling the concentration or amount of component (a) in the oral care composition, the oral care composition can generate a drug effective concentration in the range of 1.35-13 mM in the oral cavity after being applied to the oral cavity, can more effectively inhibit Porphyromonas gingivalis, has no substantial influence on Lactobacillus reuteri and the total amount of the bacterial flora in the oral cavity of healthy people, and thus can effectively improve the microenvironment of the oral bacterial flora and the imbalance of the bacterial flora, and further avoid or prevent oral diseases.

The present invention discloses the application of oral microbiota in the diagnosis of heart failure. The present invention has for the first time discovered a combination of biomarkers related to the diagnosis of heart failure, namely Streptococcus, Neisseria, Neisseria subflava, Veillonella parvula, Neisseria cinerea, Haemophilus parainfluenzae, Neisseria meningitidis, Geotrichum candidum, Haemophilus influenzae, and Veillonella massiliensis. By detecting the abundances of the biomarkers in the oral microbiota of a subject, heart failure can be diagnosed, thereby better intervening in the occurrence and development of heart failure and guiding clinicians to provide prevention or treatment plans for the subject.

The invention discloses a rapid evaluation method of host caries risk based on Raman spectrum, and relates to the technical field of microbiological detection. Aiming at the problem that in the prior art, it is difficult to rapidly and quantitatively reflect the early metabolic activity of the oral flora, the method introduces a stable isotope signal through heavy water labeling to obtain single-cell Raman spectrum information of the oral flora, and evaluates the metabolic activity of the flora on the basis of a characteristic parameter C-D ratio reflecting the metabolic state of microorganisms in a Raman spectrum, so that the early metabolic activity of the oral flora can be rapidly and quantitatively reflected. Therefore, the judgment of the caries risk of the host is realized. The method comprises the steps of sample collection, flora growth and metabolism law research under different concentration heavy water labeling, and metabolic activity detection under different environmental conditions. By adopting the heavy water concentration of 40%, on the premise that flora growth is not obviously influenced, rapid characterization of metabolic activity can be realized in a short time, the resolution ratio of single cells is high, and disturbance is small. The method provides a new tool for early warning of caries and oral flora metabolism research, and has a wide application prospect.

This invention relates to the field of antimicrobial peptide technology, specifically to an antimicrobial peptide for maintaining oral flora balance, its preparation method, and its application. The antimicrobial peptide for maintaining oral flora balance according to this invention includes any one of the following polypeptides (a)-(e): (a) a polypeptide whose amino acid sequence includes the amino acid sequence shown in SEQ ID NO. 1; (b) an amino acid sequence composed of amino acid residues shown in SEQ ID NO. 1; (c) a polypeptide formed by substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence defined in (a) or (b); (d) a polypeptide having 99% or more, 95% or more, 90% or more, 85% or more, or 80% or more homology to the amino acid sequence defined in (a) or (b); (e) a polypeptide obtained by modifying the N-terminus and / or C-terminus of any one of the polypeptides defined in (a)-(d). The antimicrobial peptides of this invention, used to maintain oral flora balance, exhibit antibacterial and bactericidal activity against oral pathogens while not affecting the normal growth of beneficial bacteria. This balance is maintained, protecting gum health and freshening breath. These antimicrobial peptides are suitable for preparing oral antibacterial agents and related oral care products, regulating the balance of the oral microecology, and thus used to prevent and treat oral diseases, especially dental caries, periodontitis, oral mucosal diseases, and oral cancer. The antimicrobial peptides of this invention are relatively short, making chemical synthesis easier and allowing for direct synthesis into high-purity products. They also exhibit clear effects and have wide applications.

The invention discloses a micro-ecological protective oral cleaning composition for whole-course control of dental calculus and application of the micro-ecological protective oral cleaning composition, and belongs to the technical field of oral care products. The composition contains citrate, a multienzyme mixture (at least two of lysozyme, xylanase and chymotrypsin) and a matrix additive. Citrate is chelated with calcium ions to soften and dissolve calcified dental calculus; enzymolysis of a multi-enzyme mixture inhibits early bacterial attachment and sediment accumulation and cooperates with citrate to decompose calcified dental calculus; the matrix additive ensures stable release of active ingredients. The components are synergistic, the whole process from the initial stage of dental calculus formation to calcification maturation intervenes, inhibits deposition and dissolves a calcification structure, and meanwhile, the composition can protect the micro-ecology of the oral cavity and has no obvious destructive effect on oral flora. The composition is applied to preparation of oral care products, can solve the problems of one-sided cleaning effect, poor hidden area effect and dysbacteriosis caused by sterilization of existing products, achieves the dual purposes of whole-course dental calculus control and micro-ecological maintenance, and is wide in application prospect.

The application discloses application of Geotrichum candidum as a biomarker in heart failure diagnosis. The application studies the correlation between oral flora and heart failure, and finds through detection of clinical samples that the Geotrichum candidum can be used as an independent biomarker for diagnosing heart failure. Meanwhile, the application provides a biomarker combination comprising the Geotrichum candidum, and the biomarker combination has high heart failure diagnosis efficiency. The application can realize early diagnosis of heart failure, guide clinicians to provide a prevention or treatment scheme for a subject, and thus better intervene in the disease process of heart failure occurrence and development.

The present invention discloses an oral flora collection device that can be remotely controlled, comprising a body and a handle connected in a pistol shape; the front end of the body is connected to a collection component through an adjustment rod; a lighting bulb and a camera are provided at the bottom of the collection component, and a bacteria collection brush head is detachably connected to the bottom of the collection component; a display screen connected to the camera and a screen switch for controlling the display screen are installed on the body; a lighting switch for controlling the lighting bulb and a camera switch for controlling the camera are provided on the handle, and a power supply device electrically connected to the camera and the display screen is installed inside the handle. The present invention has the functions of visualization and aseptic replacement of the brush head, can realize simple operation by a single person, overcomes the problems of limited field of vision in the collection of existing oral flora and easy secondary infection of the brush head, and is more conducive to the treatment and care of oral diseases.

The invention discloses application of geotrichum candidum as a biomarker in diagnosis of heart failure. The correlation between oral flora and heart failure is studied, and clinical sample detection shows that geotrichum candidum can be used as an independent biomarker for diagnosing heart failure. Meanwhile, the invention provides a biomarker combination containing geotrichum candidum, and the biomarker combination has relatively high heart failure diagnosis efficiency. According to the invention, early diagnosis of heart failure can be realized, and a clinician is guided to provide a prevention or treatment scheme for a subject, so that the disease progress of occurrence and development of heart failure can be better intervened.

To provide an oral composition that selectively and efficiently promotes growth of Neisseria and / or Rothia species, non-pathogenic bacteria present in the oral microbiota, enabling effective improvement of the oral microbiota.SOLUTION: An oral composition comprising the following components (A) and (B): (A) a nitrate ion-supplying compound and (B) a chlorophyll metal complex salt derivative.SELECTED DRAWING: None

The present invention discloses a longan pro-inflammatory protein, an extraction method and an application thereof. The pro-inflammatory protein is a longan protein extract extracted by the phenol extraction method. The longan pro-inflammatory protein of the present invention can promote the growth of oral inflammatory pathogenic bacteria. When culturing oral flora, it can specifically promote the growth of pathogenic bacteria without promoting the growth of other non-pathogenic bacteria, providing an experimental basis for simulating the environment of massive reproduction of pathogenic bacteria in the oral cavity. At the same time, the longan pro-inflammatory protein of the present invention can also promote the growth of pathogenic bacteria in the intestine and promote the expression of inflammatory factors in cells at a relatively low concentration, providing a basis for subsequent pharmaceutical research and development.

The present invention discloses a predictive index for the efficacy of immunotherapy for lung cancer and its application, and belongs to the technical field of predictive markers. The present invention provides a predictive index for the efficacy of immunotherapy for lung cancer, and the predictive index includes a specific oral flora. The present invention also discloses the application of a quantitative detection reagent for the predictive index in the preparation of a lung cancer prognosis prediction reagent. The present invention analyzes salivary microbiome and metabolite data based on non-targeted metabolomics liquid chromatography mass spectrometry (LC / MS) of Metagenomic Next‑Generation Sequencing (mNGS). Through multi-omics analysis, it is found that the distribution of specific oral flora has an important regulatory effect on immunotherapy resistance, and it is confirmed that its related fat metabolism is highly enriched in lung cancer patients with immunotherapy resistance.

The present invention relates to compositions and their use for oral care. In particular, the compositions and methods are for maintaining oral health and / or treating various oral conditions such as gingivitis. The present invention relates to methods and uses of stabilized amorphous calcium phosphate (ACP) and / or stabilized amorphous calcium fluoride phosphate (ACFP) in the preparation of a medicament for reducing pathogenic oral bacteria at an oral site in an individual; increasing commensal oral bacteria at an oral site in an individual; decreasing the proportion of pathogenic oral bacteria at an oral site in an individual; inhibiting oral dysbiosis; reducing gingival inflammation in an individual in need thereof; treating gingivitis in an individual in need thereof; and treating chronic gingivitis in an individual in need thereof.

The invention relates to the technical field of probiotics, in particular to pediococcus pentosaceus with the effect of preventing or treating decayed teeth and periodontal diseases as well as a preparation and application of the pediococcus pentosaceus. The pediococcus pentosaceus is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 15, 2023, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 27356. The pediococcus pentosaceus provided by the invention can remarkably inhibit growth and reproduction of oral pathogens such as streptococcus mutans, porphyromonas gingivalis, fusobacterium nucleatum, actinomycetes viscosity, actinobacillus actinomycetes and the like, is beneficial to oral flora balance, and can be widely used for preventing or treating oral diseases such as decayed teeth and periodontitis.

The invention discloses a polypeptide dental surface antifouling agent as well as a preparation method and application thereof, and belongs to the technical field of dental care preparations. The polypeptide dental surface antifouling agent comprises a polypeptide synthesized by the following sequences: (a) a dental surface anchoring peptide sequence as shown in SEQ ID NO.1; (b) a zwitterionic peptide sequence; and (c) a connecting peptide sequence as shown in SEQ ID NO.2, wherein the connecting peptide sequence is used for connecting the dental face anchoring peptide sequence and the zwitterionic peptide sequence. The polypeptide dental surface antifouling agent disclosed by the invention has good cytocompatibility and can be quickly and firmly combined with the dental surface to form a hydration barrier layer, so that the adhesion of protein and bacteria can be effectively prevented, and the problem of unbalance / disturbance of oral flora is avoided while the formation of a dental biofilm is inhibited; meanwhile, continuous flushing of saliva can be resisted, the effect of preventing protein and bacterial adhesion for a long time in the oral environment can be achieved, frequent washing is not needed to maintain the antibacterial activity, use is convenient, and the antifouling performance is excellent.

To provide an oral composition that selectively and effectively promotes the growth of bacteria belonging to the genus Neisseria within the oral microbiota.SOLUTION: An oral composition comprises the following components (A) and (B): (A) a nitrate ion-supplying compound, and (B) an organic acid salt in an amount of 0.4 mass% or more and 18 mass% or less.SELECTED DRAWING: None

To provide a solid food product capable of effectively improving the oral flora in an oral cavity while using a plant-derived powder.SOLUTION: The present invention pertains to a solid food product comprising the following components (A) and (B): (A) a plant-derived powder containing a nitrate salt; and (B) one or more salt compounds selected from organic acid salts (B1) and carbonate compounds (B2).SELECTED DRAWING: None

To provide an oral cosmetic which prevents dental caries, gingivitis, periodontitis or the like by maintaining a good balance of bacterial flora in the mouth and is stable over a long period of storage.SOLUTION: There is provided an oral cosmetic which contains, as prebiotics, a herb oil extracted from herbs, humic shale or plant minerals extracted from humic shale, EM-X which is an active fermentation ingredient, a plant-derived antibacterial or bactericidal agent and an antioxidant. In addition, the oral cosmetic further contains an interbacterial nutrient network control component and a Th17 cell activation prevention component. The herbal oil is extracted by a low-temperature extractive distillation method. The biochemical effects are diverse and suggest a contribution to the maintenance and improvement of oral health. The biochemical effects are diverse and suggests the contribution to the maintenance and improvement of oral health. Thus, a product containing herbal raw materials is likely to be useful in oral care and can be combined with conventional care methods to achieve more effective care.SELECTED DRAWING: Figure 1

Health authorities recommend the use of iron drops as a supplement for each infant at the age of 6 months and encourage and monitor their use until the age of 2 years. In addition, the use of iron drops becomes a long-term necessity in all systemic conditions that cause iron deficiency. Iron discoloration is quite common in children, especially in the infant period (0- 2 years) due to the structure of the milk teeth and the incomplete oral flora. The developed toothpaste for infants and children comprising phytic acid will eliminate iron-related discoloration of milk teeth.

To provide an oral microbiota improver that selectively and efficiently promotes growth of Neisseria and / or Rothia species in the oral microbiota, enabling effective improvement of the oral microbiota.SOLUTION: An oral microbiota improver comprising, as an active ingredient, one or two selected from chlorophyll metal complex salt derivatives and chlorophyll derivatives.SELECTED DRAWING: None