Methods are provided for detecting
antigen binding agents in samples. Aspects of the methods include detection of the aggregation of
antigen binding agents with
polynucleotide-bound antigens by sensitive proximity-based association of the
antigen-bound
polynucleotide s. Aspects of the methods also include methods for the detection of such proximity-based association through
nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.