32 results about "Particle agglutination" patented technology

A homogeneous, non-competitive, double receptor agglutination assay for measuring immunosuppressant drugs is described. The assay employs at least two receptors wherein each receptor is specific for a separate binding site on the drug and wherein each receptor is bound to a detection particle. The immunosuppressant drug binds to the receptors and causes particle agglutination, which can be measured and correlated with the presence or amount of immunosuppressant drug in a sample.

A method of treating particles to be used in immunoassays reduces interference in particle agglutination assays. For particles having covalently bound antibodies and residual NHS-ester or sNHS-ester groups on the surface, the reactive esters are treated with an aqueous mixture containing an amine compound of formula (I):

H₂N—R—X  (I).

The moiety —X is —NH₂, —OH, or —CO₂CH₂CH₃; and R is an alkyl group or an alkyl ether group. When —X is —NH₂ or —CO₂CH₂CH₃, R contains from 1 to 20 carbon atoms; and when —X is —OH, R contains from 4 to 20 carbon atoms.

A latex reagent for analyzing adiponectin, comprising a suspension of latex particles carrying a substance which specifically binds to adiponectin, is disclosed. Further, a method for analyzing adiponectin, comprising (1) obtaining a biological liquid possibly containing adiponectin, and (2) bringing the biological liquid, while maintaining the state in which the biological liquid is obtained, into contact with a suspension of latex particles carrying a substance which specifically binds to adiponectin, and optically analyzing a degree of latex-particles-agglutination, is disclosed. According to the latex reagent and the method for analyzing adiponectin, a predilution or pretreatment of the biological liquid to be analyzed is not necessary. Further, the analysis can be performed rapidly and conveniently, and facilities therefor are not limited.

A method for the detection and/or visualization of particle agglutination, comprising: placing a volume of a suspension of the particles on a filter, the filter being constructed so as to permit passage of individual particles; placing a volume of a solution or suspension containing an agglutinating agent at the location of the particle suspension; optionally placing a wash solution at the location of the agglutinating agent; and observing the surface at the location for the presence of particles, such presence indicating that agglutination of the particles occurred. There is also provided a method for detection of agglutination reactions in general and hemagglutination reactions, such as used in blood grouping and cross-matching, in particular. The method is comprised of successive vertical additions of whole blood, blood grouping reagent and wash to a filter. In case of hemagglutination a colored, preferably red or reddish dot becomes visible after washing. A device and a kit based on the invention is also claimed and facilitates blood grouping and matching in non-laboratory environment without the need for laboratory instruments.

The invention discloses an inert carrier indirect agglutination test detection system and application thereof. The system comprises inert carrier bacteria S9 and a complex S9-P which is shown and expressed on the surface of the inert carrier bacteria S9 and carries a P-antibody factor. The system only carries the P-antibody factor, is single in component and specific in targeting, generates macroscopic positive particle agglutination reaction with whole blood or serum of chicken infected by pullorum disease and salmonella typhimurium under a certain concentration condition, and does not generate non-specific cross agglutination reaction with chicken-derived serum and whole blood of different backgrounds infected by non-pullorum disease and salmonella typhimurium. The S9-P is based on a glass plate agglutination reaction operation platform. The S9-P is simple and convenient to operate, sensitive and rapid in reaction, macroscopic in agglutination reaction particles and clear and easy inresult judgment, tests and result judgment are completed within two minutes, and the S9-P is suitable for targeted specific detection of pullorum disease and salmonella gallinarum infection in chicken flocks and has a good application prospect in on-site monitoring and diagnosis of the chicken flocks.

Latex particles for a particle agglutination assay, with which it is possible to carry out high-sensitivity assay while highly suppressing a non-specific reaction are disclosed. The latex particles being obtained by emulsion polymerization with a polymerizable monomer having a phenyl group, and a polymerizable monomer having a phenyl group and a sulfonate in an aqueous medium including a nonionic surfactant at a concentration of 0.005 to 0.02 wt % based on the aqueous medium, the latex particles having an average particle size of 0.005 to 1.0 μm.

Turbidimetric immunoassay of insoluble carrier particles that can inhibit the agglutination reaction of insoluble carrier particles such as latex by inhibiting the effect of plasma components that affect the measured value, stabilize the agglutination reaction, stabilize the absorbance of the reaction solution, and obtain accurate measurement results A reagent for measurement, a kit for insoluble carrier particle turbidimetric immunoassay, and a method for insoluble carrier particle turbidimetric immunoassay using the reagent or kit. In the presence or absence of a buffer containing a compound containing the following groups in the molecule, such as broad bean pyrimidine glucoside, tricoflavone, etc., the insoluble carrier particles are loaded with antibodies or antigens, and then, in the presence of the above buffer , the insoluble carrier particle suspension sensitive to the antibody or antigen is in contact with the test body, and an immunoagglutination reaction occurs, and the concentration produced is measured through the agglutination reaction of the insoluble carrier particles, and the antigen or antibody in the test body is quantified. (In the formula, R1, R2, and R3 can be the same or different, and represent a hydrogen atom, a hydroxyalkyl group, etc.)

The invention belongs to the bird immunology technology area, it concretely relates to a kit of acceptable for the one type duck hepatitis virus antibody emulsion agglutination and the application of testing one type duck hepatitis virus antibody emulsion agglutination. The kit of this invention contains box body and core reagent in the box body, positive check sample, negative check sample, sample cell, scale sample sucker and the carrier of observed result. The characteristic is that core reagent is latex antigen from sublimating e duck hepatitis virus AV2111 and inactivating one type duck hepatitis virus sensitization polystyrene latex, the positive check sample is one type duck hepatitis virus antiserum, the negative check sample is the green duck of a day old of no one type duck hepatitis mother source antibody. The carrier is the paper characteristic latex agglutination card of having hollowness. The invention also discloses the preparation method and application of this kit. The kit has the significance merit of high specificity, high sensitivity, handy operation, and quickly diagnose.

The invention provides a rebamipide-containing aqueous pharmaceutical suspension which can be prepared by a simple process and keep the dispersed fine-particle state of rebamipide stable without having the fine particle agglutinated. The rebamipide-containing aqueous pharmaceutical suspension of the invention is prepared by mixing polyvinyl alcohol and additionally a sodium salt compound with rebamipide.

A particle agglutination-evaluating container for immunological analysis, based on an agglutinate formed in an agglutination reaction between an antibody- or antigen-containing sample and agglutination particles, includes a transparent container body having a bottom face including a sloping bottom face and a raised bottom face extended in a horizontal direction at the top of the sloping bottom face to form an obtuse angle with the sloping bottom face, and a fluidal separation layer containing insoluble particles which are filled in the container body and where the agglutination particles are separated according to the agglutination degree, wherein agglutination is evaluated by observation from the bottom of the container body, where the agglutination reaction is judged positive when the agglutination particles are observed through the raised bottom face, negative when observed at the bottom end of the sloping bottom face, and weakly positive when observed in the middle of the sloping bottom face.

This invention relates to quinidine derivatives and immunogenic conjugates and reporter conjugates prepared therefrom. The immunogenic conjugates and reporter conjugates are useful for eliciting antibodies and for performing immunoassays for quinidine. A particle agglutination immunoassay for quinidine is also provided.

A mixing method for particle agglutination includes the following steps of: dropping testing materials into an accommodating recess at one end of a channel structure; pressing a flexible layer on the other end of the channel structure; and releasing the flexible layer to an initial position after pressing the flexible layer such that a negative pressure is generated by an air chamber that is covered by the flexible layer and draws the testing materials that are in the accommodating recess to move toward the air chamber along a diverging channel of the channel structure. The testing materials are mixed with each other in the diverging channel, in which the depth of the diverging channel is gradually increased from the accommodating recess to the air chamber.