The invention relates to the technical field of analytical chemistry, and discloses an analysis method for determining rebamipide related substances by using HPLC. According to the analysis method, octadecylsilane chemically bonded silica is used as a reversed phase chromatographic column of a filler, a buffer salt solution is used as a mobile phase A, the buffer salt solution, methanol and tetrahydrofuran are used as a mobile phase B, gradient elution is carried out on a rebamipide sample solution, and HPLC analysis is carried out. The analysis method provided by the invention can effectivelyseparate rebamipide and related substances thereof, so that impurity peaks and rebamipide peaks are not overlapped, the peak shape is good, the separation requirement is met, and the method is suitable for controlling related substances and researching impurities.