1026 results about "Osteo arthritis" patented technology

The invention discloses a preparation method of injectable nano composite hydrogel for relieving osteoarthritis, which comprises the following steps: firstly, synthesizing nano rod-like hydroxyapatite with the length of 150-300 nm and the diameter of 10-15 nm, and the hydroxyapatite is hydrophilic hydroxyapatite doped with strontium and europium ions; the preparation method comprises the following steps: by taking Ca (NO3) 2, Sr (NO3) 2, Eu (NO3) 3 and soluble phosphate as main raw materials, carrying out hydrothermal reaction to prepare strontium and europium ion co-doped hydrophobic hydroxyapatite, dispersing the strontium and europium ion co-doped hydrophobic hydroxyapatite in a Tris-HCl buffer solution, adding tannic acid, stirring, centrifugally collecting precipitate, and drying to obtain strontium and europium ion doped hydrophilic hydroxyapatite; the preparation method comprises the following steps: taking chondroitin sulfate and adipic dihydrazide as main raw materials, stirring, dialyzing, freeze-drying, and dissolving in a buffer solution to obtain the chondroitin sulfate solution grafted with the hydrazide group; the preparation method comprises the following steps: taking hyaluronic acid, 3-aminophenylboronic acid and sodium periodate as main raw materials, stirring, dialyzing, freeze-drying, and dissolving in a buffer solution to obtain the phenylboronic acid group grafted oxidized hyaluronic acid solution; the preparation method comprises the following steps: blending hydrophilic hydroxyapatite doped with strontium and europium ions, a chondroitin sulfate solution grafted with a hydrazide group, an oxidized hyaluronic acid solution grafted with a phenylboronic acid group and a recombinant collagen solution, and stirring to obtain the injectable nano composite hydrogel. The injectable nano composite hydrogel obtained by the invention has good biocompatibility, and a favorable local immune microenvironment can be created to immunomodulate macrophages so as to relieve osteoarthritis and promote subchondral bone repair.

The invention relates to the technical field of pharmaceutical chemicals, and discloses sequential drug release liposome hydrogel and a preparation method and application thereof, and the preparation method of the liposome hydrogel comprises the following steps: step 1, preparation of drug-loaded positive charge liposome; step 2, preparing phenylboronic acid modified polysaccharide; and step 3, carrying out vortex mixing on the drug-loaded positive charge liposome solution, the phenylboronic acid modified polysaccharide hydrogel precursor powder and the spermidine solution to obtain the liposome hydrogel. According to the sequential drug release liposome hydrogel as well as the preparation method and the application thereof, the liposome hydrogel is prepared by taking high-molecular hyaluronic acid and phenylboronic acid as main raw materials, and the liposome hydrogel has microenvironment responsiveness and time-space characteristics of drug release, and can be used for treating osteoarthritis in an environment with active oxygen increase, so that the drug release effect is good, and the drug release effect is good. The functions of early-stage anti-inflammation and continuous cartilage regeneration are achieved by sequentially releasing double drugs, synovitis can be effectively relieved, cartilage regeneration can be promoted, and the compound can be used for preparing drugs for treating osteoarthritis.

The invention provides application of an enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury, and belongs to the technical field of biological medicine. The treatment activity of the umbilical cord mesenchymal stem cell exosome is remarkably improved by combining a composite pretreatment technology with a 3D culture system. Through the synergistic effect of the total flavonoids of rhizoma drynariae and tanshinone IIA, the pathological process of knee osteoarthritis can be regulated and controlled in multiple dimensions, including inflammatory reaction inhibition, cartilage repair promotion and joint function improvement, and the functional limitation of single-component pretreatment is broken through. The invention provides a safe and efficient novel treatment technical strategy for relieving knee osteoarthritis and muscle cartilage injury, and has a wide clinical application prospect.

The invention relates to the technical field of medical informatics, in particular to a knee osteoarthritis traditional Chinese medicine treatment scheme optimization method based on big data analysis, and the method comprises the following steps: based on knee joint load distribution data, collecting knee joint shear stress data, monitoring cartilage stress gradient data, and calculating a stress peak value in a gait cycle; and obtaining a knee joint biomechanical load balance value. According to the method, by combining shear stress, cartilage stress gradient and gait cycle stress peak value calculation, individual biomechanical characteristics are accurately described, patellar tendon stretching and quadriceps femoris tendon displacement data are analyzed, the instantaneous elastic modulus is recognized, tendon adaptability adjustment measurement precision is improved, soft tissue elastic change is reflected, and by means of individualized stress characteristic data, the stability of the muscle tendon is improved. Abnormal data are eliminated, illness state analysis is optimized, the curative effect evaluation accuracy is improved, the rehabilitation training intensity is dynamically optimized according to the curative effect offset degree, the therapeutic scheme is more adaptive, and the curative effect predictability and the personalized level are improved.

The invention relates to a knee osteoarthritis (KOA) multi-classification method based on multi-modal fusion, and the method fuses the characteristics of three modals of two different magnetic resonance imaging (MRI) sequences and X-ray films to carry out four classifications on knee osteoarthritis grades, namely normal, mild, moderate and severe. According to the method, a three-dimensional convolutional neural network (3D CNN) is designed to respectively extract three-dimensional shallow layer features of two different MRI sequences and fuse the features, then a dependency relationship between feature channels after fusion is captured through an efficient channel attention (ECA) module, and the dependency relationship is input into a Vision Transformer (ViT) encoder, so that the MRI sequence of the two different MRI sequences can be identified, and the MRI sequence of the two different MRI sequences can be identified. In combination with the advantages of the 3D CNN and the ViT, the local features and the global features of the two MRI sequences are efficiently aggregated, and the output of the ViT is spliced with the X-ray image features extracted by the two-dimensional convolutional neural network, so that the classification performance is further improved. According to the method, through fusion of three pieces of modal information, the model can capture different physiological and anatomical information, and more reliable support is provided for diagnosis of knee osteoarthritis.

The invention relates to the technical field of medical data processing, in particular to a knee osteoarthritis acupuncture curative effect prediction system based on deep learning. The system obtains acupuncture treatment parameters and acupuncture effect parameters of historical knee osteoarthritis patients; the historical knee osteoarthritis patients are divided into patient categories based on the acupuncture effect parameters, and the contribution degree is obtained according to the distribution of each acupuncture treatment parameter in each patient category and the distribution difference between the acupuncture treatment parameter and the acupuncture treatment parameter in other patient categories; acquiring a local curative effect feedback value according to the contribution degree and the acupuncture effect parameter, and further acquiring an evaluation score; and training the acupuncture curative effect prediction model through the acupuncture treatment parameters, the evaluation scores and the acupuncture effect parameters to obtain a trained acupuncture curative effect prediction model. According to the method, the evaluation score is dynamically obtained, the participation degree of each acupuncture treatment parameter of each historical knee osteoarthritis patient is accurately determined, and the accuracy of acupuncture treatment effect prediction model training is effectively improved.

The invention belongs to the technical field of biological medicines, and discloses a DSH loaded medicine as well as a preparation method and application thereof. The DSH loading type medicine is DNA (Deoxyribonucleic Acid) supramolecular hydrogel loaded with 2, 2, 6, 6-tetramethylpiperidine-1-oxide, and the DSH loading type medicine is a DNA supramolecular hydrogel loaded with the 2, 2, 6, 6- Wherein the DNA supramolecular hydrogel is formed by assembling a Y type DNA monomer and an L type DNA monomer. The DSH loaded medicine has good anti-inflammatory and peroxy free radical removing effects, and can achieve the purpose of treating knee osteoarthritis. In addition, the DSH loaded medicine can be used for reflecting the content of peroxy free radicals in knee joints by detecting the contrast ratio of knee joint images through magnetic resonance, and early diagnosis of knee osteoarthritis is achieved.

The invention belongs to the technical field of biomedical anti-inflammatory gels, and discloses a renewable boundary lubrication natural polyphenol anti-inflammatory hydrogel and a preparation method and application thereof.The hydrogel is of a hydrophilic viscoelastic sea island structure, gallic acid grafted gelatin blended with hyaluronic acid serves as a base material, celecoxib lipidosome is entrapped in the hydrogel, and the hydrogel is prepared from natural polyphenols, an articular cavity is injected through an enzyme crosslinking technology to form gel, hyaluronic acid and lipidosome form a hydrated lubricating layer on a cartilage-gel interface, and after the interface is abraded, internal lipidosome and hyaluronic acid are exposed to supplement lubrication so as to form sustainable lubrication; the liposome responds to friction shear crushing to release celecoxib and cooperates with gallic acid at an interface to resist inflammation progress and repair cartilage, the injectable hydrogel is developed based on gallic acid, hyaluronic acid, gelatin and celecoxib liposome to treat early osteoarthritis, mechanical lubrication and inflammation resistance are combined, and the preparation method has the advantages that the preparation method is simple, the preparation cost is low, and the application prospect is wide. The problems of large-dose toxicity, short lubrication period and the like caused by independent treatment are effectively solved, and the preparation has important clinical application significance.

The invention belongs to the technical field of biomedical materials, and particularly relates to a gradient bionic wood-based collagen bone-cartilage repair scaffold and a preparation method and application thereof.The scaffold comprises a surface layer, a middle layer and a bottom layer, the surface layer is composed of collagen, the middle layer is composed of collagen and nano-hydroxyapatite, and the bottom layer is composed of collagen and nano-hydroxyapatite; the bottom layer is composed of wood, collagen and nano-hydroxyapatite, the scaffold is obtained by stacking the surface layer, the middle layer and the bottom layer and then conducting freeze-drying, the structure of the scaffold is similar to that of natural osteochondral cartilage, the surface layer is of a porous structure communicated with one another, and the bottom layer is of a directionally-arranged pore channel structure; the main composition of a natural bone cartilage tissue can be highly simulated, and the gradient is presented; the bone marrow mesenchymal stem cell prepared by the method has the advantages of high mechanical performance, gradient enhancement from the surface layer to the bottom layer, excellent biocompatibility and biological activity, capability of remarkably promoting cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells, and application to osteochondral tissue regeneration and clinical treatment of osteoarthritis.

The invention provides a mechano-electric coupling hydrogel microsphere with stable mechanical property and low loss transduction as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering bioremediation materials. According to the invention, supramolecular engineering and microfluidic technologies are utilized, and a slip ring structure polyrotaxane and conductive polypyrrole network is introduced to construct stress-electric coupling hydrogel microspheres. The molecular slippage mechanism of the slip ring structure stores and releases mechanical energy to reduce mechanical loss, and the conjugated pi-electron movement in the conductive network improves the electron transfer efficiency in the microsphere, so that the loss in stress-electricity conversion is reduced for the first time. Compared with the traditional piezoelectric hydrogel microspheres, the stress-electric coupling efficiency of the low-dissipation microspheres is improved by 2.3 times, and the energy dissipation rate is reduced to 43%. The mechano-electric coupling hydrogel microspheres can reduce cartilage injury of rats, improve behavior results and promote treatment of osteoarthritis by recovering low-loss transduction between tissues.

The invention relates to the field of osteoarthritis drug treatment, in particular to application of RuO2 nano-enzyme in preparation of a drug for treating osteoarthritis, and particularly relates to knee osteoarthritis. The RuO2 nano-enzyme disclosed by the invention is prepared according to the following method: carrying out incubation deposition on Cu2O sphere nano-particles and RuCl3 to obtain Cu2O and RuO2, and etching Cu2O and RuO2 spheres to obtain the RuO2 nano-enzyme. The RuO2 nano-enzyme provided by the invention is a hollow RuO2 nano-enzyme sphere, and after the RuO2 nano-enzyme is horizontally used in an animal body for intervention, the hollow RuO2 nano-enzyme sphere can be used as a hollow RuO2 nano-enzyme sphere; the RuO2 nano-enzyme can effectively delay the development of knee osteoarthritis of a mouse knee osteoarthritis model caused by medial meniscus instability (DMM), improve the pain condition and movement function of a DMM modeling mouse, reduce the gonarthromeningitis degree and relieve knee joint cartilage degeneration and injury, and the effectiveness of the RuO2 nano-enzyme in delaying the knee osteoarthritis disease is further proved. The invention provides a novel method for treating knee osteoarthritis, and has strong practicability and application prospect.

The invention discloses a recombinant VI-type collagen as well as a preparation method and application thereof. The recombinant VI-type collagen comprises a protein gene segment derived from a natural VI-type collagen alpha2 chain, and proteins P0607, P060702 and / or P060703 capable of promoting cartilage cell proliferation activity are expressed. Gene segments derived from a natural type VI collagen alpha2 chain are integrated through a genetic engineering means, sequence optimization and repetition are carried out on the gene segments to enhance the biological activity of the collagen, the recombinant type VI collagen P0607, P060702 and P060703 can be stably and efficiently produced, the recombinant collagen has the effect of promoting chondrocyte proliferation, and the recombinant collagen can be applied to the field of cartilage cell proliferation. An efficient and reliable biological manufacturing way is provided for research, development and production of osteoarthritis medicines.

The invention discloses a medicine for treating osteoarthritis, and belongs to the field of medicine, the medicine comprises a reagent capable of stimulating chondrocyte development, and the medicine L-arginine can promote expression of chondrocyte anabolic factors and reduce expression of cartilage degradation markers, so that chondrocytes are protected to a certain extent; in addition, the medicine provided by the invention has an excellent cartilage repair function, can inhibit the progress of degenerative osteoarthritis, has good biocompatibility, and has no cytotoxicity to cartilage cells.

The invention discloses a knee joint fraction prediction method and system fused with multi-modal image features. The knee joint fraction prediction method fused with the multi-modal image features obtains a knee joint prediction fraction of an object through five steps. The method has the beneficial effects that 1, deep learning, radiomics characteristics and combined clinical characteristics are fused, the magnetic resonance T1 sequence image of the knee joint is processed and analyzed, the popliteal artery ROI is marked, and related radiomics and deep network characteristics are extracted; compared with traditional single feature analysis, the method has the advantages that more comprehensive and accurate disease assessment is provided, and the accuracy and reliability of knee osteoarthritis prediction are improved. And 2, the method is based on the obvious relation between the popliteal artery and the knee bone joint, innovatively analyzes the image features of the popliteal artery and combines clinical data, so that the method not only can predict the occurrence and progress of the osteoarthritis more accurately in an earlier stage, but also obviously improves the prediction effect.

The invention relates to the technical field of preparation of astaxanthin sustained-release microspheres, and discloses chitosan-based astaxanthin sustained-release microspheres and a preparation method thereof.The method comprises the steps that chitosan powder is dissolved in an acetic acid solution, the pH value is adjusted, and a chitosan solution is prepared; dissolving astaxanthin in ethanol, and then adding the chitosan solution; adding a cross-linking solution into the emulsified solution, and performing cross-linking under a magnetic stirring condition to form astaxanthin sustained-release microspheres; adding the astaxanthin sustained-release microspheres into a strengthening solution for strengthening treatment; carrying out coating treatment on the astaxanthin sustained release microspheres subjected to the strengthening treatment; carrying out centrifugal treatment on the solution subjected to coating treatment, and collecting astaxanthin sustained-release microspheres subjected to centrifugal separation; and washing the collected astaxanthin sustained-release microspheres with PBS (Phosphate Buffer Solution). According to the invention, chitosan is used as a carrier to prepare the astaxanthin sustained-release microspheres, so that the astaxanthin can be continuously and stably released in intestinal tracts, the antioxidant and anti-inflammatory activity of the astaxanthin can be fully exerted, and further, the astaxanthin sustained-release microspheres have an effective treatment effect on osteoarthritis.

The invention provides a small extracellular vesicle composite collagen hydrogel injection, and belongs to the technical field of osteoarthritis, and the small extracellular vesicle composite collagen hydrogel injection is prepared by the following steps: S1, mixing a sodium alginate solution with a type I collagen solution to obtain a mixed solution, adding a CaCl2 solution into the mixed solution to form gel, adding an FGF18 solution into the gel, and mixing to obtain FGF18 sodium alginate / collagen hydrogel; s2, transfecting a Lenti-IGF-1-EGF lentiviral vector to P3 generation human umbilical cord mesenchymal stem cells, culturing to obtain P5 generation IGF-1 overexpressed MSCs cells, namely P5 generation IGF-1-MSCs cells, co-culturing with the P5 generation IGF-1-MSCs cells by using curcumin, and separating and extracting a small extracellular vesicle solution rich in curcumin; and S3, mixing the small extracellular vesicle solution rich in curcumin with the FGF18 sodium alginate / collagen hydrogel, so as to obtain the small extracellular vesicle composite collagen hydrogel injection. The invention provides a small extracellular vesicle composite collagen hydrogel injection, which achieves the purposes of relieving inflammatory injury of osteoarthritis and enhancing the treatment effect of osteoarthritis.

The invention relates to the field of disease animal model construction, and particularly discloses a postmenopausal osteoporosis and knee osteoarthritis co-disease animal model construction method which comprises the following steps: selecting a female animal as an experimental subject; the selected female animals are injected with a GnRH agonist, meanwhile, low-calcium feed feeding and oxidative stress stimulation are conducted, and feeding is conducted for a preset time; detecting bone mineral density and bone trabecula parameters of the fed female animals, and screening qualified female animals meeting set standards; performing meniscus tearing operation on the qualified female animal, and implanting sustained release microspheres loaded with inflammatory factors at the torn part during the operation; carrying out weight-bearing training on the female animal after the operation, and continuing for a set time length; and performing health detection on the female animals at different time points. According to the method, through multi-factor collaborative induction of the PMOP, minimally invasive construction of the KOA and multi-dimensional health detection, accurate simulation of a co-disease pathological process and clinical characteristics is realized, and the authenticity and stability of the model are improved.

The invention relates to the technical field of biological medicines, in particular to an umbilical cord source mesenchymal stem cell exosome preparation, a preparation method and application in preparation of osteoarthritis medicines. The preparation is prepared by the method. The method comprises the following steps: pretreating umbilical cord-derived mesenchymal stem cells by adopting a pretreating agent; removing the pretreatment agent, and carrying out gradient centrifugation treatment on the pretreated umbilical cord source mesenchymal stem cells to obtain exosome precipitate; and re-suspending the exosome precipitate in a buffer solution containing a protease inhibitor to obtain the umbilical cord source mesenchymal stem cell exosome preparation. The application refers to the application of the preparation in preparation of osteoarthritis drugs. The problems of low yield and functional heterogeneity of EXO obtained by a conventional culture method are solved.

The invention belongs to the technical field of medicines, and discloses a meloxicam cataplasm and a preparation method thereof. The meloxicam cataplasm is composed of a backing layer, a paste layer and a protective layer, wherein the paste layer comprises an active component meloxicam, an adhesive, a humectant, a drug dissolution promoter, a transdermal enhancer, a surfactant, a pH regulator, a preservative and the balance of water for injection. The preparation method comprises the following steps: dissolving an adhesive, a transdermal enhancer and a preservative in water for injection, stirring and uniformly mixing to serve as a matrix phase; uniformly mixing the meloxicam with the humectant, adding the drug dissolution promoter and the surfactant to dissolve the active ingredients, and uniformly stirring to obtain a liquid medicine phase; the preparation method comprises the following steps: transferring a matrix phase and a liquid medicine phase into a closed vacuum tank, stirring and mixing in vacuum, adding a pH regulator, continuously stirring to obtain an ointment, uniformly coating a backing material with the ointment, covering the upper surface with a protective layer, and drying at low temperature to obtain the meloxicam cataplasm, which can relieve inflammation symptoms of soft tissue injury, such as pain, swelling and the like. The medicine is used for treating osteoarthritis.

The invention relates to a cartilage stem cell for promoting joint repair and application thereof. In particular, the present invention provides a cellular preparation for alleviating or treating osteoarthritis. The cell line comprises Procr-positive pluripotent stem cells. The novel cartilage stem cell provided by the invention can be differentiated in vivo to generate downstream cartilage cells, and cartilage repair in osteoarthritis injury is promoted; it is prompted that selection of Procr positive cartilage stem cells for cell treatment will be a more effective treatment method. And the Procr protein can be used as a new target of the medicine for treating osteoarthritis.

The invention provides a nanogel for rapid permeation of articular cartilage and responsive release of a growth factor, which is prepared by polymerizing a peptide fragment which can be cut by a substance (mainly enzyme) which can be specifically expressed on the surface of osteoarthritis cells or secreted into a matrix with the growth factor, an acrylamide monomer and a cationic micromolecule monomer. Unreacted monomer molecules are filtered out through an ultrafiltration tube or other filtering systems, so that the nanogel which can rapidly permeate into the deep part of articular cartilage and can be recognized and cut by enzyme highly expressed on the surface of osteoarthritis cells or secreted enzyme to release growth factors is prepared, and the effect of the growth factors can be more effectively exerted; the nanogel has strong cartilage infiltration capacity, can completely infiltrate cartilage with the thickness of 1mm within less than 3 days, can be cut by MT1-MMP protein specifically expressed on the surfaces of osteoarthritis cells, releases growth factors, is specifically combined with growth factor receptors on the surfaces of cartilage cells, and activates downstream signal channels, so that the proliferation of the cartilage cells is promoted, and the cartilage cell proliferation rate is increased. The cartilage matrix secretion is promoted, so that the cartilage matrix thickening effect is realized, and the osteoarthritis is prevented and treated.

The invention provides an external traditional Chinese medicine composition for treating knee osteoarthritis and a preparation method thereof. The traditional Chinese medicine composition is prepared from Chinese angelica, safflower, pollen typhae, herba schizonepetae, zedoary, radix clematidis, notopterygium root, cortex periplocae, cortex erythrinae, caulis spatholobi, fried szechwan chinaberry fruit, rhizoma smilacis glabrae, salvia miltiorrhiza, pepper, garden balsam stem and prepared kusnezoff monkshood root. The external traditional Chinese medicine composition for treating knee osteoarthritis is reasonable in compatibility of the components, dredges channels and collaterals, activates blood circulation to remove blood stasis, enables qi and blood of tendons and vessels to be smooth, eliminates dampness and coldness, and relieves pain, so that the treatment purpose is achieved.

The invention provides a porous magnetic hyaluronic acid microneedle for treating knee osteoarthritis and a preparation method, and relates to the technical field of biological materials. The porous magnetic hyaluronic acid microneedle for treating knee osteoarthritis comprises a needle body and a matrix layer, the needle body is a porous drug-loading autolysis hyaluronic acid microneedle body, and the matrix layer is a magnetic hyaluronic acid matrix. The invention has the following beneficial effects: 1, a soluble biocompatible microneedle structure is adopted, so that the injury caused by the breakage of a metal microneedle is avoided, and the medicine can directly reach the subcutaneous part; 2, a porous structure is constructed through ice crystal recrystallization, and the drug loading content is increased through a secondary drying technology; 3, the drug carrier is added through the nano magnetic particles, so that diffusion and absorption of drug components are promoted; 4, the operation is simple, tedious steps such as injection in the articular cavity are not needed, and the infection risk in the articular cavity is reduced; 5, batch mold production can be realized, and the production cost is reduced; 6, the administration amount is increased through microneedle perforation administration and matrix layer permeation micropore administration.

The invention discloses a knee arthritis image measurement model system and method based on deep learning, particularly relates to the technical field of image processing, and is used for solving the problem of low image measurement accuracy. According to the method, the image preprocessing technology and the deep learning segmentation method are integrated, high-precision automatic segmentation and analysis are performed on the knee joint image, the contrast ratio and the resolution ratio of the image are enhanced, meanwhile, the image quality is further improved through biased field correction and non-local mean value noise reduction, and the image segmentation is performed by adopting the U-Net model, so that the image quality is improved. A complex structure in the joint is effectively recognized, segmentation precision is improved, a segmentation strategy is dynamically adjusted according to measurement influence information generated in the segmentation process, the processing flow is optimized in real time through different regulation and control signals, diagnosis efficiency and accuracy are improved, and therefore the automation level and reliability of medical image processing are improved, and the medical image processing efficiency is improved. Efficient and accurate support is provided for clinic, and the accuracy of osteoarthritis image measurement is improved.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a bone arthralgia removing composition for treating knee osteoarthritis as well as a preparation method and application of the bone arthralgia removing composition. The traditional Chinese medicine composition is prepared from 5-15 parts of cassia twig, 5-15 parts of radix clematidis, 5-15 parts of Chinese prickly ash, 5-15 parts of radix saposhnikoviae, 5-15 parts of radix achyranthis bidentatae, 5-15 parts of lycopodium clavatum, 5-15 parts of garden balsam stem, 5-15 parts of fructus liquidambaris, 5-15 parts of cortex erythrinae, 5-15 parts of ligusticum wallichii, 5-15 parts of obscured homalomena rhizome and 5-15 parts of cowherb seed. The traditional Chinese medicine composition can improve the effect of treating knee osteoarthritis.

The disclosure relates to compounds of formula I, wherein X, and R1-R4, n, and m have the meaning given in the specification, and pharmaceutically acceptable salts thereof. The compounds of formula I exhibit GPR 183 antagonistic activity and are thus useful in the treatment or prevention of disorder, condition, or disease where GRP183 antagonist is indicated to be useful, such as e.g. chronic pain e.g osteoarthritis, chronic low back pain, neuropathic pain and immune system diseases e.g inflammatory bowel diseases e.g. Colitis and Chron's diseases, multiple sclerosis, rheumatoid arthritis, inflammatory pulmonary diseases e.g. asthma and psoriasis.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The present invention provides a Fuzi decoction granule with detoxification and retained efficacy and a preparation method thereof, belonging to the technical field of traditional Chinese medicine preparation. The preparation method of the Fuzi decoction granule of the present invention comprises: soaking Fuzi and decocting Fuzi in advance for 60 to 120 minutes, decocting Fuzi with soaked Poria, ginseng, bran-fried Atractylodes macrocephala, white peony root, red peony root and cinnamon twig for 30 to 60 minutes, and filtering the filtrate to obtain the Fuzi decoction granule after concentrating and granulating. The Fuzi decoction granule obtained by the preparation method of the present invention can remove toxicity and retain efficacy on osteoarthritis.

The invention provides a hydrogel microsphere with high elasticity and super-lubrication performance as well as a preparation method and application of the hydrogel microsphere, and belongs to the technical field of biomedical materials. Through electrostatic / hydrogen bond interaction, hectorite nanosheets are uniformly dispersed in a GelMA base segment in an intercalation structure, so that the elasticity of the microspheres is enhanced. Meanwhile, the multilayer structure of the hectorite creates a physical limitation of'living and movable 'through the space between the filling layers, so that the free movement of water molecules is limited. Then, PMPC is grafted to the surfaces of the microspheres through a surface graft polymerization technology, a hydrated lubricating layer is formed through interaction with active and dynamic charge dipoles of water molecules, and finally construction of the degradable elastic and lubricating hydrogel microspheres is achieved. The hydrogel microspheres disclosed by the invention have excellent performance in the aspects of reducing cartilage friction and slowing down the progress of osteoarthritis, and pave a way for application of hydrogel in the fields of pharmacy, medical treatment, mechanical lubrication and the like.

The invention provides methods and compositions for treatment of pain, such as joint pain, using capsaicin in a procedure that attenuates transient burning sensation experienced by patients due to capsaicin administration. The methods desirably provide relief from joint pain, such as osteoarthritic knee joint pain, for an extended duration, such as at least about 3 months, 6 months, 9 months, or 1 year. To attenuate the adverse side effect of a transient burning sensation caused by capsaicin-induced neuronal excitation, the methods utilize a cooling article, such as a material wrap cooled via a circulating fluid, to reduce the temperature of tissue to be exposed to capsaicin to within a certain range for certain durations of time, optionally in combination with administering a local anesthetic agent, resulting in the substantial reduction or even elimination of transient burning sensation caused by capsaicin.