773 results about "Osteo arthritis" patented technology

The invention provides application of an enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury, and belongs to the technical field of biological medicine. The treatment activity of the umbilical cord mesenchymal stem cell exosome is remarkably improved by combining a composite pretreatment technology with a 3D culture system. Through the synergistic effect of the total flavonoids of rhizoma drynariae and tanshinone IIA, the pathological process of knee osteoarthritis can be regulated and controlled in multiple dimensions, including inflammatory reaction inhibition, cartilage repair promotion and joint function improvement, and the functional limitation of single-component pretreatment is broken through. The invention provides a safe and efficient novel treatment technical strategy for relieving knee osteoarthritis and muscle cartilage injury, and has a wide clinical application prospect.

The invention relates to the technical field of medical informatics, in particular to a knee osteoarthritis traditional Chinese medicine treatment scheme optimization method based on big data analysis, and the method comprises the following steps: based on knee joint load distribution data, collecting knee joint shear stress data, monitoring cartilage stress gradient data, and calculating a stress peak value in a gait cycle; and obtaining a knee joint biomechanical load balance value. According to the method, by combining shear stress, cartilage stress gradient and gait cycle stress peak value calculation, individual biomechanical characteristics are accurately described, patellar tendon stretching and quadriceps femoris tendon displacement data are analyzed, the instantaneous elastic modulus is recognized, tendon adaptability adjustment measurement precision is improved, soft tissue elastic change is reflected, and by means of individualized stress characteristic data, the stability of the muscle tendon is improved. Abnormal data are eliminated, illness state analysis is optimized, the curative effect evaluation accuracy is improved, the rehabilitation training intensity is dynamically optimized according to the curative effect offset degree, the therapeutic scheme is more adaptive, and the curative effect predictability and the personalized level are improved.

The invention relates to the technical field of medical data processing, in particular to a knee osteoarthritis acupuncture curative effect prediction system based on deep learning. The system obtains acupuncture treatment parameters and acupuncture effect parameters of historical knee osteoarthritis patients; the historical knee osteoarthritis patients are divided into patient categories based on the acupuncture effect parameters, and the contribution degree is obtained according to the distribution of each acupuncture treatment parameter in each patient category and the distribution difference between the acupuncture treatment parameter and the acupuncture treatment parameter in other patient categories; acquiring a local curative effect feedback value according to the contribution degree and the acupuncture effect parameter, and further acquiring an evaluation score; and training the acupuncture curative effect prediction model through the acupuncture treatment parameters, the evaluation scores and the acupuncture effect parameters to obtain a trained acupuncture curative effect prediction model. According to the method, the evaluation score is dynamically obtained, the participation degree of each acupuncture treatment parameter of each historical knee osteoarthritis patient is accurately determined, and the accuracy of acupuncture treatment effect prediction model training is effectively improved.

The invention belongs to the technical field of biological medicines, and discloses a DSH loaded medicine as well as a preparation method and application thereof. The DSH loading type medicine is DNA (Deoxyribonucleic Acid) supramolecular hydrogel loaded with 2, 2, 6, 6-tetramethylpiperidine-1-oxide, and the DSH loading type medicine is a DNA supramolecular hydrogel loaded with the 2, 2, 6, 6- Wherein the DNA supramolecular hydrogel is formed by assembling a Y type DNA monomer and an L type DNA monomer. The DSH loaded medicine has good anti-inflammatory and peroxy free radical removing effects, and can achieve the purpose of treating knee osteoarthritis. In addition, the DSH loaded medicine can be used for reflecting the content of peroxy free radicals in knee joints by detecting the contrast ratio of knee joint images through magnetic resonance, and early diagnosis of knee osteoarthritis is achieved.

The invention belongs to the technical field of biomedical anti-inflammatory gels, and discloses a renewable boundary lubrication natural polyphenol anti-inflammatory hydrogel and a preparation method and application thereof.The hydrogel is of a hydrophilic viscoelastic sea island structure, gallic acid grafted gelatin blended with hyaluronic acid serves as a base material, celecoxib lipidosome is entrapped in the hydrogel, and the hydrogel is prepared from natural polyphenols, an articular cavity is injected through an enzyme crosslinking technology to form gel, hyaluronic acid and lipidosome form a hydrated lubricating layer on a cartilage-gel interface, and after the interface is abraded, internal lipidosome and hyaluronic acid are exposed to supplement lubrication so as to form sustainable lubrication; the liposome responds to friction shear crushing to release celecoxib and cooperates with gallic acid at an interface to resist inflammation progress and repair cartilage, the injectable hydrogel is developed based on gallic acid, hyaluronic acid, gelatin and celecoxib liposome to treat early osteoarthritis, mechanical lubrication and inflammation resistance are combined, and the preparation method has the advantages that the preparation method is simple, the preparation cost is low, and the application prospect is wide. The problems of large-dose toxicity, short lubrication period and the like caused by independent treatment are effectively solved, and the preparation has important clinical application significance.

The invention discloses a knee joint fraction prediction method and system fused with multi-modal image features. The knee joint fraction prediction method fused with the multi-modal image features obtains a knee joint prediction fraction of an object through five steps. The method has the beneficial effects that 1, deep learning, radiomics characteristics and combined clinical characteristics are fused, the magnetic resonance T1 sequence image of the knee joint is processed and analyzed, the popliteal artery ROI is marked, and related radiomics and deep network characteristics are extracted; compared with traditional single feature analysis, the method has the advantages that more comprehensive and accurate disease assessment is provided, and the accuracy and reliability of knee osteoarthritis prediction are improved. And 2, the method is based on the obvious relation between the popliteal artery and the knee bone joint, innovatively analyzes the image features of the popliteal artery and combines clinical data, so that the method not only can predict the occurrence and progress of the osteoarthritis more accurately in an earlier stage, but also obviously improves the prediction effect.

The invention relates to the technical field of preparation of astaxanthin sustained-release microspheres, and discloses chitosan-based astaxanthin sustained-release microspheres and a preparation method thereof.The method comprises the steps that chitosan powder is dissolved in an acetic acid solution, the pH value is adjusted, and a chitosan solution is prepared; dissolving astaxanthin in ethanol, and then adding the chitosan solution; adding a cross-linking solution into the emulsified solution, and performing cross-linking under a magnetic stirring condition to form astaxanthin sustained-release microspheres; adding the astaxanthin sustained-release microspheres into a strengthening solution for strengthening treatment; carrying out coating treatment on the astaxanthin sustained release microspheres subjected to the strengthening treatment; carrying out centrifugal treatment on the solution subjected to coating treatment, and collecting astaxanthin sustained-release microspheres subjected to centrifugal separation; and washing the collected astaxanthin sustained-release microspheres with PBS (Phosphate Buffer Solution). According to the invention, chitosan is used as a carrier to prepare the astaxanthin sustained-release microspheres, so that the astaxanthin can be continuously and stably released in intestinal tracts, the antioxidant and anti-inflammatory activity of the astaxanthin can be fully exerted, and further, the astaxanthin sustained-release microspheres have an effective treatment effect on osteoarthritis.

The invention provides a small extracellular vesicle composite collagen hydrogel injection, and belongs to the technical field of osteoarthritis, and the small extracellular vesicle composite collagen hydrogel injection is prepared by the following steps: S1, mixing a sodium alginate solution with a type I collagen solution to obtain a mixed solution, adding a CaCl2 solution into the mixed solution to form gel, adding an FGF18 solution into the gel, and mixing to obtain FGF18 sodium alginate / collagen hydrogel; s2, transfecting a Lenti-IGF-1-EGF lentiviral vector to P3 generation human umbilical cord mesenchymal stem cells, culturing to obtain P5 generation IGF-1 overexpressed MSCs cells, namely P5 generation IGF-1-MSCs cells, co-culturing with the P5 generation IGF-1-MSCs cells by using curcumin, and separating and extracting a small extracellular vesicle solution rich in curcumin; and S3, mixing the small extracellular vesicle solution rich in curcumin with the FGF18 sodium alginate / collagen hydrogel, so as to obtain the small extracellular vesicle composite collagen hydrogel injection. The invention provides a small extracellular vesicle composite collagen hydrogel injection, which achieves the purposes of relieving inflammatory injury of osteoarthritis and enhancing the treatment effect of osteoarthritis.

The invention relates to the field of disease animal model construction, and particularly discloses a postmenopausal osteoporosis and knee osteoarthritis co-disease animal model construction method which comprises the following steps: selecting a female animal as an experimental subject; the selected female animals are injected with a GnRH agonist, meanwhile, low-calcium feed feeding and oxidative stress stimulation are conducted, and feeding is conducted for a preset time; detecting bone mineral density and bone trabecula parameters of the fed female animals, and screening qualified female animals meeting set standards; performing meniscus tearing operation on the qualified female animal, and implanting sustained release microspheres loaded with inflammatory factors at the torn part during the operation; carrying out weight-bearing training on the female animal after the operation, and continuing for a set time length; and performing health detection on the female animals at different time points. According to the method, through multi-factor collaborative induction of the PMOP, minimally invasive construction of the KOA and multi-dimensional health detection, accurate simulation of a co-disease pathological process and clinical characteristics is realized, and the authenticity and stability of the model are improved.

The invention relates to the technical field of biological medicines, in particular to an umbilical cord source mesenchymal stem cell exosome preparation, a preparation method and application in preparation of osteoarthritis medicines. The preparation is prepared by the method. The method comprises the following steps: pretreating umbilical cord-derived mesenchymal stem cells by adopting a pretreating agent; removing the pretreatment agent, and carrying out gradient centrifugation treatment on the pretreated umbilical cord source mesenchymal stem cells to obtain exosome precipitate; and re-suspending the exosome precipitate in a buffer solution containing a protease inhibitor to obtain the umbilical cord source mesenchymal stem cell exosome preparation. The application refers to the application of the preparation in preparation of osteoarthritis drugs. The problems of low yield and functional heterogeneity of EXO obtained by a conventional culture method are solved.

The invention provides a nanogel for rapid permeation of articular cartilage and responsive release of a growth factor, which is prepared by polymerizing a peptide fragment which can be cut by a substance (mainly enzyme) which can be specifically expressed on the surface of osteoarthritis cells or secreted into a matrix with the growth factor, an acrylamide monomer and a cationic micromolecule monomer. Unreacted monomer molecules are filtered out through an ultrafiltration tube or other filtering systems, so that the nanogel which can rapidly permeate into the deep part of articular cartilage and can be recognized and cut by enzyme highly expressed on the surface of osteoarthritis cells or secreted enzyme to release growth factors is prepared, and the effect of the growth factors can be more effectively exerted; the nanogel has strong cartilage infiltration capacity, can completely infiltrate cartilage with the thickness of 1mm within less than 3 days, can be cut by MT1-MMP protein specifically expressed on the surfaces of osteoarthritis cells, releases growth factors, is specifically combined with growth factor receptors on the surfaces of cartilage cells, and activates downstream signal channels, so that the proliferation of the cartilage cells is promoted, and the cartilage cell proliferation rate is increased. The cartilage matrix secretion is promoted, so that the cartilage matrix thickening effect is realized, and the osteoarthritis is prevented and treated.

The invention provides a porous magnetic hyaluronic acid microneedle for treating knee osteoarthritis and a preparation method, and relates to the technical field of biological materials. The porous magnetic hyaluronic acid microneedle for treating knee osteoarthritis comprises a needle body and a matrix layer, the needle body is a porous drug-loading autolysis hyaluronic acid microneedle body, and the matrix layer is a magnetic hyaluronic acid matrix. The invention has the following beneficial effects: 1, a soluble biocompatible microneedle structure is adopted, so that the injury caused by the breakage of a metal microneedle is avoided, and the medicine can directly reach the subcutaneous part; 2, a porous structure is constructed through ice crystal recrystallization, and the drug loading content is increased through a secondary drying technology; 3, the drug carrier is added through the nano magnetic particles, so that diffusion and absorption of drug components are promoted; 4, the operation is simple, tedious steps such as injection in the articular cavity are not needed, and the infection risk in the articular cavity is reduced; 5, batch mold production can be realized, and the production cost is reduced; 6, the administration amount is increased through microneedle perforation administration and matrix layer permeation micropore administration.

The invention discloses a knee arthritis image measurement model system and method based on deep learning, particularly relates to the technical field of image processing, and is used for solving the problem of low image measurement accuracy. According to the method, the image preprocessing technology and the deep learning segmentation method are integrated, high-precision automatic segmentation and analysis are performed on the knee joint image, the contrast ratio and the resolution ratio of the image are enhanced, meanwhile, the image quality is further improved through biased field correction and non-local mean value noise reduction, and the image segmentation is performed by adopting the U-Net model, so that the image quality is improved. A complex structure in the joint is effectively recognized, segmentation precision is improved, a segmentation strategy is dynamically adjusted according to measurement influence information generated in the segmentation process, the processing flow is optimized in real time through different regulation and control signals, diagnosis efficiency and accuracy are improved, and therefore the automation level and reliability of medical image processing are improved, and the medical image processing efficiency is improved. Efficient and accurate support is provided for clinic, and the accuracy of osteoarthritis image measurement is improved.

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a bone arthralgia removing composition for treating knee osteoarthritis as well as a preparation method and application of the bone arthralgia removing composition. The traditional Chinese medicine composition is prepared from 5-15 parts of cassia twig, 5-15 parts of radix clematidis, 5-15 parts of Chinese prickly ash, 5-15 parts of radix saposhnikoviae, 5-15 parts of radix achyranthis bidentatae, 5-15 parts of lycopodium clavatum, 5-15 parts of garden balsam stem, 5-15 parts of fructus liquidambaris, 5-15 parts of cortex erythrinae, 5-15 parts of ligusticum wallichii, 5-15 parts of obscured homalomena rhizome and 5-15 parts of cowherb seed. The traditional Chinese medicine composition can improve the effect of treating knee osteoarthritis.

The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.

The invention relates to the technical field of biological medicines, and discloses a polypeptide for treating osteoarthritis by aiming at AHR (Aryl Hydrocarbon Receptor), the amino acid sequence of the polypeptide is GFQQSDVIHQSVYELISINHTEDRA, and the amino acid sequence of the polypeptide is GFQQSDVIHQSVYELISINHTEDRA, and the amino acid sequence of the polypeptide is GFQQSDVIHQSVYELISINHTEDRA. The polypeptide is prepared by a solid-phase polypeptide synthesis method; the solid-phase polypeptide synthesis method specifically comprises the following steps: synthesizing from a C end to an N end, connecting a carboxyl group of a first amino acid to insoluble resin, then adding other amino acids one by one to form a peptide chain, and carrying out protection and deprotection reactions of a side chain in each step. The polypeptide is composed of natural amino acids and has good biocompatibility and degradability, and experimental results show that no obvious toxic reaction occurs in the cell level and the animal level, so that the polypeptide has conditions for further development of pharmacokinetic research and preclinical tests, and a feasible basis is provided for conversion to clinical application.

The invention provides a combined use of a hyaluronic acid-bilirubin conjugate and honokiol in preparation of a medicine for treating osteoarthritis. The invention provides a honokiol-loaded nanoparticle, which is characterized in that a hyaluronic acid-bilirubin conjugate and honokiol are used as raw materials to prepare a nanoparticle HKL-PNPs, and then the nanoparticle HKL-PNPs is modified by a mitochondrial targeting peptide to obtain the honokiol-loaded nanoparticle HKL-MPNPs. The invention also provides a bionic nanoparticle for treating bone joints. The bionic nanoparticle is prepared by wrapping the honokiol-loaded nanoparticle with an engineered cartilage cell membrane (ECM). According to the invention, cartilage cells with high expression of IL-1R2 are obtained through a gene editing method for the first time, and mitochondrial targeted nanoparticle cores loaded with Sirtuin-3 agonists are coated by cell membranes of the cells, so that the bionic nano preparation is obtained and is used for treating OA. The prepared bionic nano preparation not only can neutralize IL-1beta mediated inflammatory reaction, but also can recover Sirtuin-3 mediated mitochondrial function imbalance, so that the result of synergistic treatment of OA is achieved.

The invention belongs to the technical field of food and medicine, and relates to application of corn germ concentrated powder in improvement of osteoarthritis. The invention particularly relates to application of corn germ concentrated powder, namely corn antioxidant multienzyme, in preparation of medicines or health-care foods for treating rheumatoid arthritis and osteoporosis. In the corn germ concentrated powder, superoxide dismutase / (U / g) is greater than or equal to 8500, glutathione peroxidase / (U / g) is greater than or equal to 5000, and catalase / (U / g) is greater than or equal to 750. The corn germ concentrated powder can also be prepared by the following method: cleaning corn, germinating, adding water into the germinated corn, grinding and pulping, adding a settling agent into the corn steep liquor, standing, taking supernate, adding part of sodium ascorbate, performing temperature-controlled concentration, adding the rest sodium ascorbate and a stabilizer into the concentrated solution, and uniformly mixing to obtain the corn germ concentrated powder. And drying at controlled temperature, crushing, and mixing with a proper amount of silicon dioxide. The prepared corn germ concentrated powder has the effect of resisting rheumatoid arthritis.

The application provides an improved hyaluronic acid injection preparation for inflammation targeting and stem cell capture, a preparation method and use thereof, and belongs to the field of medicine. The injection preparation is prepared from unsaturated bond modified hyaluronic acid, stem cell targeting DNA tetrahedron and thiol modified inflammation targeting aptamer as raw materials. The injection preparation can be targeted to accumulate at an osteoarthritis site, capture mesenchymal stem cells in situ, recruit mesenchymal stem cells to the osteoarthritis site, promote differentiation of stem cells into cartilage, promote cartilage repair, play a role in treating osteoarthritis, and has a good application prospect.

The invention provides a nano-drug carrier and a drug release system, the nano-drug carrier comprises a drug loading core and a responsive shell arranged outside the drug loading core, and the drug loading core is loaded with a nano-drug; the surface of the responsive shell is modified with a targeting ligand, and the targeting ligand can specifically target pain-related cells or tissues. According to the nano-drug carrier and the drug release system provided by the invention, precise long-acting treatment of chronic pain is realized through combination of an environment-responsive carrier and an active targeting technology. The technology can significantly improve local accumulation of the medicine and reduce systemic toxicity, and is suitable for management of intractable pains such as neuropathic pains and osteoarthritis.

The invention discloses a knee osteoarthritis dynamic grading prediction and intervention system and method based on a large model, and relates to the field of medical image analysis, and the method comprises the steps: obtaining a knee joint MRI image sequence and knee joint angle time sequence data of a patient; processing the MRI image sequence by using a pre-trained articular cavity segmentation model to obtain a joint fluid volume quantized value; aligning the knee joint angle time sequence to an image frame acquisition time point to generate a synchronous angle sequence; whether the average value of the liquid amount change rate sequence is lower than a preset stable threshold value or not is judged by calculating the liquid amount change rate sequence and the angle change quantity sequence, and if yes, a low-risk signal is output; otherwise, calculating a correlation coefficient between the liquid amount change rate and the angle change amount, outputting a moderate or severe gonitis prediction signal according to the value of the correlation coefficient, and further judging the mild or moderate risk of the gonitis according to the amplitude characteristics of the liquid amount change rate sequence; according to the invention, early gonitis lesion features can be identified, and the accuracy of grading prediction is improved.

Disclosed herein are methods of treatment of pain associated with osteoarthritis, pain associated with osteonecrosis, and pain associated with one or more subchondral insufficiency fractures; methods of improving physical function in a human subject with osteoarthritis, osteonecrosis, or one or more subchondral insufficiency fractures; and methods of treating osteoarthritis, or delaying or slowing the progression of osteoarthritis, or relieving a symptom of osteoarthritis, in humans with these diseases, comprising administering a therapeutically effective amount of a composition comprising a glycosylated p75NTR neurotrophin binding protein (NBP)-Fc fusion protein.

Described herein are methods and compositions for using microbial agents (probiotics) and agents that promote growth of certain microbes (prebiotics) for management (including prevention and treatment) of musculoskeletal disorders, including osteoporosis, osteopenia, Paget's disease, stunting, osteoarthritis, osteomyelitis, and delayed or non-union fractures. Also described herein are methods and compositions for using probiotics and prebiotics for management of inflammation, and symptoms of menopause.

The invention belongs to the technical field of bioengineering and application thereof, and particularly relates to a laver compound zymolyte as well as a preparation method and application thereof. The compound enzyme is prepared through a compound enzymolysis process of protease and laver polysaccharide enzyme. Compared with a traditional single proteolysis process, a composite enzymolysis process of adding the high-enzyme-activity laver polysaccharide enzyme effectively breaks a'polysaccharide-protein 'composite structure of laver cell walls, so that the viscosity of zymolyte is reduced, high laver liquefaction rate and high product yield are realized, and the release efficiency of nutritional active ingredients is remarkably improved; the product is rich in low-molecular-weight polypeptide and oligomeric porphyra polysaccharide. The obtained zymolyte shows the comprehensive effects of improving joint activity, inhibiting osteophyte formation, adjusting cartilage metabolism balance and relieving inflammatory response in an osteoarthritis mouse model, and osteoarthritis is effectively relieved. According to the invention, efficient release and utilization of nutritional active ingredients of the laver are realized, and powerful technical support is provided for developing high-valued products, such as health-care products and functional foods, which have the function of maintaining joint health.

The invention discloses a specific disease iconography feature extraction method and system based on deep learning, and relates to the technical field of image analysis, and the method comprises the steps: collecting X-ray image data, stem cell markers, magnetic resonance imaging image data and dynamic ultrasonic image data of an osteoarthritis patient; performing standardization processing on the X-ray image data, the stem cell marker, the magnetic resonance imaging image data and the dynamic ultrasonic image data to obtain a standardized X-ray image data set, a standardized stem cell marker, a standardized magnetic resonance imaging image data set and a standardized dynamic ultrasonic image data set; a standardized X-ray image data set, a standardized magnetic resonance imaging image data set and a standardized dynamic ultrasound image data set are acquired. According to the invention, through multi-modal image data fusion and biomechanics-stem cell characteristic dynamic coupling, early accurate diagnosis and repair potential evaluation of osteoarthritis are realized.

The present disclosure describes devices, methods and systems for modifying or altering gait kinematics via sensory augmentation and / or modifying muscle activation patterns via augmented motor learning to slow the progression of and / or reduce the pain associated with knee OA, particularly during gait (e.g., walking, running, stair climbing, etc.). Sensors can be used to measure gait parameters and characteristics and muscle activation patterns. Stimulation can be provided to the individual in order to promote learning new gait kinematics and muscle activation patterns.

The invention provides a construction method and application of an osteoarthritis animal model. According to the construction method, a standardized experimental Beagle dog is selected, an improved Hulth method is used for establishing the osteoarthritis model, a mode of inducing the experimental dog to move is optionally adopted for further treatment, and the animal model close to the clinical actual situation is constructed. The animal model obtained through the construction method fits traumatic arthritis caused by increasingly worn joints in actual life, the model stability is good, and a new animal model with important practical value is provided for research and development of osteoarthritis drugs and medical instruments.

The invention provides application of apoptotic cell pretreated macrophages in preparation of an osteoarthritis treatment preparation, a cell preparation and a preparation method, and relates to the technical field of biomedicine.In the embodiment of the invention, based on a mouse development model and an osteoarthritis (OA) model, it is found that in the area away from the cartilage surface, the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment, and the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment in the area away from the cartilage surface; in the macrophages, the content of the interment-related LAMP1 protein is obviously higher than that of the macrophages close to the cartilage surface area, and the expression results of TGF-beta 1 and LRG1 are respectively opposite and consistent with those of the LAMP1. The invention also finds that after the chemotactic effect of apoptotic cells on macrophages is weakened, the cellular burial effect is limited, and the cartilage matrix development disorder is caused. Based on development process inspiration, the invention provides a treatment strategy: bone marrow-derived macrophages pretreated by apoptotic cells are injected into articular cavities, and a good cartilage regeneration treatment effect can be achieved in a mouse arthritis model. In addition, the bone marrow-derived macrophages pretreated by apoptotic cells can still express TGF-beta1 after being injected into the body for 3 days, so that the effect of continuously promoting cartilage regeneration is achieved.

The present application relates to an NEK7 inhibitor, a pharmaceutical composition and a use thereof. The compound has a structural formula of formula (I). The compound of the present disclosure has good NEK7 inhibitory activity, and can be used for treating or preventing inflammatory pathological disorders (Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, atopic dermatitis, ulcerative colitis, Crohn's disease, cancer, osteoarthritis, gout, etc.) regulated by the NLRP3 inflammasome.

Provided herein are quinazolinone compounds for modulating muscle myosin to treat obesity and related disorders such as cardiovascular diseases, type 2 diabetes, obstructive sleep apnea, cancer, and osteoarthritis.