187 results about "Mesenchyma" patented technology

Methods of ex-vivo expansion and at the same time inhibiting differentiation of stem cells by co-culture with mesenchymal cells, transplantable populations of renewable progenitor and stem cells expanded thereby, and their uses in therapeutic applications.

Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic tissue-like constructs can be generated from fibroblastic cells of mesenchymal origin (at least), which can be either differentiated cells or multipotent adult stem cells. In this work, tissue-like organization and macroscopic tissue-like constructs have been generated from dermal fibroblasts, adipose stromal cells-derived osteogenic cells, chondrocytes, and from osteoblasts. The factor causing macroscopic tissue formation is large scale culture at high cell seeding density per unit area or three-dimensional space, that is, macromass culture done on a large scale. No scaffold or extraneous matrix is used for tissue generation, the tissues are of completely cellular origin. No other agents (except high cell-seeding-density) that aid in tissue formation such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, etc, are employed for tissue formation. These tissue-like masses have the potential for use as tissue replacements in the human body. Tissue-like organization by high cell-seeding-density macromass culture can also be generated at the microscopic level.

The invention described herein provides a structure for growing isolated differentiable human mesenchymal cells, which includes a three-dimensional matrix of fibers. The matrix serves as an implantable scaffolding for delivery of differentiable human mesenchymal cells in tissue engineering. The invention further provides compositions that contain the three-dimensional matrix of fibers seeded with isolated differentiable human mesenchymal cells, wherein the matrix forms a supporting scaffold for growing the isolated differentiable human mesenchymal cells, and wherein the differentiable human mesenchymal cells differentiate into a mature cell phenotype. The invention further provides methods of preparing the implantable nanofiber matrix scaffolding seeded with differentiable human mesenchymal cells for use in tissue engineering.

A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

The invention provides a method for reshaping and beautifying by using tissue engineering fat regeneration technology, which is applicable to plastic surgery and reshaping and beautifying and belongs to the field of tissue engineering. The method comprises the following technical routes: directly extracting mesenchyme matrix cells with multiple differentiation capabilities from fatty tissues of a patient, culturing and propagating the mesenchyme matrix cells in vitro, wrapping a specific amount of cells and other necessary components in a hydrogel stent material, and implanting the hydrogel stent material into the corresponding part in the body of the patient. The cells are grown into fatty tissues along with degradation and absorption of the stent material so as to perform regenerative repair on the soft tissue defect of the patient. The method has the advantages that the cells of the patient do not bring immunological rejection to the patient, and the regenerative repair effect is safe, natural and durable because the used hydrogel stent material is finally degraded and absorbed in the body of the patient.

The present invention generally relates to novel preparations of mesenchymal stromal cells (MSCs) derived from hemangioblasts, methods for obtaining such MSCs, and methods of treating a pathology using such MSCs. The methods of the present invention produce substantial numbers of MSCs having a potency-retaining youthful phenotype, which are useful in the treatment of pathologies.

The invention provides a cell in-vitro culture medium for enhancing viability and angiogenesis ability after myeloid mesenchyma stem cell transplantation and a processing method thereof, wherein the culture medium is prepared by adding DMOG with the terminal concentration of 500 to 2000 [mu]mol / L to conventional cell culture medium; the method includes the followings: stable stem cell of myeloid mesenchyma after subculturing is disposed in the cell culture medium with the terminal concentration of DMOG of 500 to 2000 [mu]mol / L for cultivation for 24 to 36 hours, and the stem cell of myeloid mesenchyma after processing is used for cell transplantation. The invention has the advantage that the viability and angiogenesis ability of stem cell of myeloid mesenchyma can be efficiently improved by means of DMOG preprocessing so that the curative effect for rehabilitating tissue damage by stem cell transplantation of myeloid mesenchyma can be improved.

Provided herein are lyophilized compositions containing the secretome of cultured adipose cells, pharmaceutical compositions that additionally contain a sustained release drug delivery matrix, as wellas methods of making and using such compositions.

The invention discloses a transgenic mesenchymal stem cell which can treat radiation enteritis and a preparation method. The CXCR4 protein can be expressed highly on the surface of transgenic mesenchymal stem cell; under the chemotaxis of stromal cell derived factor which is released from the injured intestine, the homing and colonization of a large amount of transgenic mesenchymal stem cells can promote proliferation and repair of the intestinal mucosa and inhibit apoptosis; the radiation enteritis is cured. The steps of the preparation method of the transgenic mesenchymal stem cell are that : (1)the separation and cultivation of mesenchymal stem cell; (2) the preparation of CXCR4 recombinant adenovirus: full length cDNA of CXCR4 is amplified by RT-PCR; the construction of CXCR4 recombinant shuttle plasmid and skeleton plasmid; the CXCR4 recombinant adenovirus is packaged, amplified, purified and determined by titer in HEK293T cell; (3) the transfect ion of mesenchymal stem cell by using CXCR4 recombinant adenovirus.

The invention provides a TRAIL secretion mesenchyma stem cell and a purpose thereof for encephaloma treatment, and relates to the field of gene recombination and stem cell application. Particularly, the invention provides a building body for expressing a secretion type TRAIL protein soluble fragment and a slow virus expression vector containing the building body. The invention also provides the mesenchyma stem cell integrating the building body on the genome; the TRAIL protein fragment can be expressed and secreted. The invention also provides application of the building body or the vector or the mesenchyma stem cell to encephaloma treatment.