172 results about "Mesenchyme" patented technology

Tooth tissues include the pulp mesenchyme that forms the dentin and an epithelium that is responsible for enamel formation. Cells from these tissues were obtained from porcine third molars and were seeded onto a biodegradable scaffold composed of a polyglycolic acid—polylactic acid copolymer. Cell polymer constructs were then surgically implanted into the omentum of athymic nude rats so that the constructs would have a blood supply and these tissues were allowed to develop inside the rats. Infrequently, columnar epithelial cells were observed as a single layer on the outside of the dentin-like matrix similar to the actual arrangement of ameloblasts over dentin during early tooth development. Developing tooth tissues derived from such cell polymer constructs could eventually be surgically implanted into the gum of an edentulous recipient where the construct would receive a blood supply and develop to maturity, providing the recipient with a biological tooth replacement.

It is intended to provide a method of forming pancreatic β cells from mesenchymal cells characterized by comprising using mammal-origin mesenchymal cells as starting cells, culturing these cells in the presence of, for example, a pancreatic β cell-forming agent, and selecting and separating the thus obtained pancreatic βcells with the use of a gene expressed specifically in such cells as a selection marker; a remedy for glucose intolerance which comprises pancreatic β cells obtained by the above method as the active ingredient; a pancreatic β cell-forming agent such as a cytokine to be used in the above method; a method of screening a candidate compound promoting the formation of pancreatic β cells from mesenchymal cells; and a pancreatic β cell formation promoter obtained by this screening method.

The invention discloses a stem cell culture medium, an application of the stem cell culture medium and a stem cell cultivation method. Blood serum does not exist in the stem cell culture medium. The stem cell culture medium comprises amino acid, vitamin, salt, lipoid, cytokines and egg white polypeptide. The stem cell culture medium is suitable for fast cultivating stem cells which are tissue sources of human and mammal, and comprises but not is limited by fat mesenchyme stem cells, mesenchymal stem cells and umbilical cord blood stem cells. The culture medium enables increasing speed of the cells to be improved by 3-5 times, and differential potentials of the cells can not be affected. Compared with an ordinary stem cell culture medium, stem cells from different sources can be fast expanded, passage number is prolonged, and proficiency properties of the stem cells can be well kept.

The invention discloses a human placenta, umbilical cord mesenchyme stem cell bank and its making method. The method includes the following steps: the first is taking human placenta, umbilical cord to test; smashing after cleaning by phosphate buffer; adding phosphate buffer to dilute; the second is adding collagenase to digest; the third is adding phosphate buffer; the fourth is adding trypsinization; the fifth is mixing the cell from the second and fourth, centrifuging, removing supernatant, cleaning by the phosphate buffer, centrifuging, and removing supernatant; the mesenchyme stem cell are gained; the sixth is freezing them in liquid nitrogen; saving by ABO/Rh subtype and HLA subtype; setting recallable cell information file. The invention is newborn storage placenta and umbilical cord mesenchyme stem cell. It can offer mesenchyme stem cell to treat the disease for personal, family and others.

The invention provides icariin application in inducing stem cell in vitro orientation differentiates to several single type cells. The stem cell said includes embryonic stem cell, nerve stem cell and marrow mesenchyme stem cell. The single type cell includes nerve cell, bone cell, islet cells and endothelial cell. This invention also relates to application of the single type cell in preparing medicine of stem cell transplant curing nerve degenerative diseases, and its application in preparing cell differentiation agent that used to repair recovery injured nerve tissue, and its application in high efficiency drug effect screening model rebuilding and initial screening and estimating by using the model. The new applications of the icariin is extended in this invention, the fact of icariin contains pharmacy activity clarified, so it provides material basis for Chinese traditional medicine prevention and cure effect. The clarifying of drug effect mechanism provides reference for new medicine Chinese metical modern development with self-owned intellectual property right.

The present invention provides isolated pluri-differentiated human mesenchymal progenitor cells (MPCs), which simultaneously express a plurality of genes that are markers for multiple cell lineages, wherein the multiple cell lineages comprise at least four different mesenchymal cell lineages (e.g., adipocyte, osteoblast, fibroblast, and muscle cell) and wherein each of the markers is specific for a single cell lineage. The present invention also method for isolating and purifying human mesenchymal progenitor cells from Dexter-type cultures for characterization of and uses, particularly therapeutic uses for such cells. Specifically, isolated MPCs can be used for diagnostic purposes, to enhance the engraftment of hematopoietic progenitor cells, enhance bone marrow transplantation, or aid in the treatment or prevention of graft versus host disease.

The present invention relates to biomedicine technology, and is especially technological process of inducing and differentiating hemopoietic stem/ancestral cell of different sources into mature red blood cell by means of the support of stroma cell in a serum-free culture system. By means of the common culturing of stroma cell originated from embryo liver or mesenchyme stem cell originated from embryo marrow and erythron ancestral cell in different extracorporeal induction stages, the present invention realizes complete denucleation of erythrocyte to create erythrocyte with complete function. The present invention makes it possible to provide great amount of general or rare hematypic erythrocyte products for medical application.

The invention discloses a method and application for inducing human umbilical cord mesenchyme stem cells to be differentiated into testicular interstitial cells. The method comprises the following step of culturing human umbilical cord mesenchyme stem cells of patients suffering from adenovirus and carrying mice steroidogenic factor-1 genes in a DMEM-F12 culture solution containing 0.3-3ng/ml of luteinizing hormone, 200-800mu M of dibutyryl cyclic adenosine monophosphate, 5*10<-6>-5*10<-4>M of all-trans retinoic acid (ATRA), 10mU/ml of human chorionic gonadotropin and 2.4uM of adrenocorticotrophic hormone for a week. Induced by the method in the invention, the human umbilical cord mesenchyme stem cells can be differentiated into testicular interstitial cells in vitro and provides important sources of cells for treating testosterone shortage by the cell replacing method or the genetic method.

The invention provides a laser-induced thermotherapy device and system based on magnetic resonance guidance. The device comprises a work station, laser ablation equipment and a minimally invasive operation optical fiber module; the work station is used for generating an operation plan according to the pre-operation focus digital image of a patient, sending the operation plan to the laser ablationequipment, generating a real-time temperature image of the focus region by using the magnetic resonance temperature imaging technology in the operation, and controlling the laser power and the coolingpower in real time through the temperature numerical values of the focus and the peripheral health tissue; the laser ablation equipment is used for producing and regulating the laser, driving and controlling the circulation of the cooling mesenchyme; the minimally invasive operation optical fiber module performs precise conformal ablation on the regular or irregular tumor in the disease treatmentby using the laser and the cooling mesenchyme and cools the operation optical fiber module and the peripheral tissue. The regular or irregular tumor can be effectively ablated, and the ablation boundary can be real-time adjusted in the operation through the nuclear magnetism temperature imaging, thereby achieving the conformal ablation aim.

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

The invention relates to a preparation method of tissue engineering skin containing appendant organs. In the invention, the preparation method comprises the following steps of: inoculating amniotic mesenchyme stem cells, amniotic mesenchyme stem cells induced to the directions of hair papillae, amniotic epithelial cells and amniotic epithelial cells induced to the directions of the epithelia of the sweat gland into a gel solution, then inoculating the amniotic epithelial cells and the amniotic epithelial cells induced by keratinocyte in a warp direction on the surface of a structure of the hair follicle and the sweat gland after culturing by a dermal layer by induced culture forming the structure of the hair follicle and the sweat gland, and obtaining the tissue engineering skin with the structure of the hair follicle and the sweat gland after culturing. The skin has elasticity, toughness, vigorous cellular metabolism, strong multiplication capacity, sufficient secretion of extracellular matrices, tight linkage of the cuticular layer, the inophragma and the cells of the dermal layer and little possibility of falling off, enhances the success rate of transplantation, can promote the healing of a wound surface, enhances the effects of restoration and replacement and has the function of regulating the immunological rejection of a receptor; by the contained structure of the hair follicle and the sweat gland, the function of the skin is more complete; the adopted amniotic tissues are postpartum wastes and have extensive source, strong cell multiplication capability and multiplepassage frequency; and the invention is beneficial to the mass production of seed cells and lowers the industrialization cost.

The invention discloses a placenta stem cell bank construction method and a placenta tissue resuscitation method, and relates to placenta stem cell bank construction methods. The placenta stem cell bank construction method comprises the following steps: after non-bacterial cleaning treatment to placenta tissues of at least three parts, the steps of tissue peeling, further sterilizing, shearing to be thin, protecting using a refrigerant, programmed freezing, cryogenic temperature temporary storage, long term storage with nitrogen canisters, and the like are carried out, and the placenta tissue with biological activities can be preserved for a long term. The placenta tissue preserved through the method can be used for the construction of a stem cell bank, resuscitation, enzymic digestion, cultivation, expansion, quality inspection and the like are performed after selective tissue part resuscitation according to required cell types, so as to obtain placenta amniotic membrance epithelial cells, placenta amniotic membrance mesenchyme, placenta chorion mesenchymal stem cell, placenta decidua serotina mesenchymal stem cells and the like. The method provided by the invention has the advantages that operation steps are simplified, manual intervention errors are reduced, the efficiency is improved, more stem cell resources are preserved, and a basis is provided for further personalized stem cell customization.

The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal al development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.

A method for preparing chondroblast of bone marrow mesogalia stem cell with three dimensional cultivation and induction includes steps of placing bone marrow mesogalia stem cell into suspending culture device preset with culture medium and microcarrier for suspending cultivation; cloning abovesaid stem cells with agitation, adding induction culture medium of chondroblast in culture meidum containing harvested cytodex3 microcrrier and abovesaid stem cells gromn on it; and carrying out induction for chondroblast assembly from abovesaid stem cells.

The present invention discloses a method for cultivating the mesenchymal stem cells from the oral tissue. A dental pulp or gingival mucosa sample is prepared, dental pulp mesenchymal stem cells are abstracted from the dental pulp tissue of the tooth or gingival mucosa mesenchymal stem cells are abstracted from a gingival mucosa tissue, then, the cells are cultivated, the cells present the kenel of the dental pulp mesenchymal stem cells or kenel of the gingival mucosa mesenchymal stem cells, and the next cultivation and the preservation are operated. The dental pulp mesenchymal stem cells and the gingival mucosa mesenchymal stem cells abstracted through the method of the present invention have the abilities of clustering, differentiating into the substance of bone, differentiating into fat, and differentiating into nerve. The method of the present invention reduces the pollution rate, and leads the mesenchymal stem cells to be easily cultivated and preserved.

The invention discloses an umbilical cord preserving fluid for temporary preservation of umbilical cord after collection before separation. The umbilical cord preserving fluid comprises the following main components: sodium chloride, glucose, human serum albumin, potassium chloride and anhydrous calcium chloride, as well as auxiliary components: cefoperazone sodium and phenol red, wherein the main components have the effects on maintaining osmotic equilibrium of inner cells of the umbilical cord tissue, keeping the umbilical cord in a wet state and providing nutrient content for a short period of time; and the auxiliary components have the effects on preventing probable pollution and prompting happened pollution. The preserving fluid can be used for preserving the activity of mesenchyme stem cells in the umbilical cord, the umbilical cord can be effectively preserved at 2-10 DEG C for at least 72 hours, the activity of the separated mesenchyme stem cells is minimally influenced by time, the time limit from collection to preparation of the umbilical cord is greatly reduced, and all the used components meet a clinical use standard and are capable of effectively reducing the pollution probability.

The invention discloses a process for separation and culture of marrow mesenchyme stem cell, compared with the conventional methods, the invention realizes easy operation, shortened time of cell culture, high purity of mesenchyme stem cells obtained through separation, and rapid propagation. The obtained cells have homogeneous shapes of elongated shuttles. After passaging, the wall adhesion capacity of the cells is strong in 8 generation.

The invention discloses four cell types which have wide heteroplastic transplantation application prospect and can be effectively induced into induced pluripotent stem (iPS) cells. The origins of three cell types are placental tissue, namely amnion mesenchymal cell, chorion mesenchymal cell and umbilical cord mesenchymal cell; and the origin of the other one is amniotic fluid cell. Besides, the invention also discloses an induction reprogramming method for producing cells capable of producing induced pluripotent stem (iPS) cells by efficient induction, including the following steps: cDNA containing pluripotent stem cell factor is respectively introduced into the four primary culture cells; the four primary cells in which cDNA is introduced are respectively cultured on appropriate culture mediums, primary iPS is obtained and then quality of iPS is optimized on appropriate culture mediums, and cloning of pluripotent stem cell can be primarily evaluated. In the invention, three mesenchymal cells of placenta origin and amniotic fluid cell all can be induced into iPS, wherein the chorion mesenchymal cell is compared with fibroblast, efficiency of induction reprogramming is improved by over 100 times, efficiency is about 2.3%, and the efficiency is slightly higher than that of horny cell; and a means which is more effective and more pertinent is provided for building disease model, screening drug and controlling oriented differentiation.

The invention relates to a method for extracting peony essential oil from peonies. The method comprises the following steps that step 1, the peonies are picked, forced air drying is conducted on the peonies, and the dried peonies are taken out and ground; step 2, a cellulase water solution and a pectinase water solution are mixed, acetum is added, and therefore a phosphoesterases complex solution is obtained; step 3, the ground peonies are placed in a triangular flask, the phosphoesterases complex solution is added, standing is conducted, ultrasonic treatment is conducted, and standing is conducted; step 4, the obtained solution in the step 3 is added to a petroleum ether ethanol solution, backflow is conducted, digestion is conducted at the indoor temperature, and the obtained solution is transferred into a rotary evaporator for vacuum concentration. According to the method, enzyme treatment is firstly conducted on the peonies so that local changes of loosening, expansion, crashing and the like are generated in the cell walls of the peonies and the mesenchyme structures of the cell walls; then, ultrasonic treatment is conducted on the peonies for a short time, and therefore the extraction rate of the essential oil from the cells of the peonies is greatly improved.