32 results about "Decidua" patented technology

The present invention relates to placenta tissue-derived multipotent stem cells and cell therapeutic agents containing the same. More specifically, to a method for producing placenta stem cells having the following characteristics, the method comprising culturing amnion, chorion, decidua or placenta tissue in a medium containing collagenase and bFGF and collecting the cultured cells: (a) showing a positive immunological response to CD29, CD44, CD73, CD90 and CD105, and showing a negative immunological response to CD31, CD34, CD45 and HLA-DR; (b) showing a positive immunological response to Oct4 and SSEA4; (c) growing attached to plastic, showing a round-shaped or spindle-shaped morphology, and forming spheres in an SFM medium so as to be able to be maintained in an undifferentiated state for a long period of time; and (d) having the ability to differentiate into mesoderm-, endoderm- and ectoderm-derived cells. Also the present invention relates to placenta stem cells obtained using the production method. The inventive multipotent stem cells have the ability to differentiate into muscle cells, vascular endothelial cells, osteogenic cells, nerve cells, satellite cells, fat cells, cartilage-forming cells, osteogenic cells, or insuline-secreting pancreatic β-cells, and thus are effective for the treatment of muscular diseases, osteoporosis, osteoarthritis, nervous diseases, diabetes and the like, and are useful for the formation of breast tissue.

The invention discloses a placenta stem cell bank construction method and a placenta tissue resuscitation method, and relates to placenta stem cell bank construction methods. The placenta stem cell bank construction method comprises the following steps: after non-bacterial cleaning treatment to placenta tissues of at least three parts, the steps of tissue peeling, further sterilizing, shearing to be thin, protecting using a refrigerant, programmed freezing, cryogenic temperature temporary storage, long term storage with nitrogen canisters, and the like are carried out, and the placenta tissue with biological activities can be preserved for a long term. The placenta tissue preserved through the method can be used for the construction of a stem cell bank, resuscitation, enzymic digestion, cultivation, expansion, quality inspection and the like are performed after selective tissue part resuscitation according to required cell types, so as to obtain placenta amniotic membrance epithelial cells, placenta amniotic membrance mesenchyme, placenta chorion mesenchymal stem cell, placenta decidua serotina mesenchymal stem cells and the like. The method provided by the invention has the advantages that operation steps are simplified, manual intervention errors are reduced, the efficiency is improved, more stem cell resources are preserved, and a basis is provided for further personalized stem cell customization.

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

Provided are a method of culturing pluripotent stem cells in the presence of a decidua-derived cell or an extracellular matrix derived from the cell, that enables safe and efficient maintenance culture and derivation of pluripotent stem cells; a culture agent for pluripotent stem cells, that comprises a decidua-derived cell or an extracellular matrix derived from the cell; and other means for developing or performing the method.

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.

The invention discloses a systematic method for preparing and cryopreserving placental tissues according to a structure layer. The method comprises the following steps: (1) separation and cryopreservation of a placental amnion and a decidua; (2) separation and cryopreservation of placenta subchorionic large vascular tissues; 3) separation and cryopreservation of the placental chorionic tissues; and (4) treatment and cryopreservation of the placental chorionic tissues. The invention also provides a method for resuscitation after cryopreservation. The placental amnion, the decidua, the subchorionic large vascellum, the chorion and other tissues are respectively separated and preserved, so the improvement of the activity of cryopreserved tissues and cells is facilitated, the morphology, the function and the structure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in the tissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells, and also can be used in the field of tissue engineering transplantation. The systematic method for preserving various tissues of the complete placenta is provided to provide a great biological source for the studying of placenta-derived stem cells.

The invention discloses a screening method for differentially expressed proteins of heterotopic-pregnancy and non-pregnancy decidua tissue. The method is characterized by comprising the following steps: carrying out proteomics analysis to discover specific proteins and differential proteins of heterotopic-pregnancy and non-pregnancy decidua tissue; subjecting the specific proteins and differential proteins to separation and purification; and using the purified specific proteins and differential proteins as special biological markers for discrimination of intrauterine and extrauterine pregnancy. After pregnancy, specific proteins can be synthesized in the bodies of both a mother and a fetus. Detection of the specific proteins with different methods is beneficial for diagnosis of pregnancy. Searching for a special marker for pregnancy, especially for distinguishing intrauterine and extrauterine pregnancy, is of critical significance to determination of clinical medical liabilities and medical jurisprudence and is a great breakthrough to the basic theory of medical science.

The invention discloses a method for obtaining maternal mesenchymal stem cells from placenta. The method for obtaining the maternal mesenchymal stem cells from the placenta comprises the following steps: obtaining placenta tissues from a position, which are within 5cm away from the radius of an umbilical cord and are 0.5 cm away from a fetal surface, of the placenta; and carrying out enzyme digestion treatment on placenta tissues by adopting an enzyme digestion solution, and then carrying out cell culture on a product of the enzyme digestion treatment to obtain the maternal mesenchymal stem cells. According to the method disclosed by the invention, the technical problem of separating the maternal mesenchymal stem cells from the placenta is solved, the prepared maternal mesenchymal stem cells are large in quantity and high in purity, neonatal cells are not easy to mix, and the clinical application requirements can be met; the method provided by the invention solves the problems that thequantity of the maternal mesenchymal stem cells obtained from the decidua basalis is small and the decidua basalis is easy to lose, and provides a new scheme and way for obtaining the maternal mesenchymal stem cells from the placenta.

The invention discloses a preparation and cryopreservation method of human placental chorionic tissues. The method comprises the following steps: (1) cleaning a placenta, and cutting off a shed decidua along the edge of the placenta to remove the amnion; and (2) separating the amnion-removed placenta fetus chorion and large vessels to obtain remaining placenta tissues which are placental chorionictissues, cutting the placental chorionic tissues to form small blocks, cutting the small blocks to form slices, placing the slices in a cryopreservation bag or a cryopreservation tube, guiding a vitrification cryopreservation solution through a three-step process, transferring the obtained mixture into a programmable cooler, reducing the pressure to -80 to -90 DEG C, transferring the cooled mixture to liquid nitrogen, and performing cryopreservation. The cryopreservation method facilitates the improvement of the activity of cryopreserved tissues and cells, the morphology, the function and thestructure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in the tissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells and epithelial cells, and also can be used in the field of tissue engineering transplantation.

The invention belongs to the field of modern pharmacy of traditional Chinese medicines, and relates to application of a specific monomer leonurine in preparation of a medicine for improving infertility pregnancy outcome. The leonurine is injected into the abdominal cavity during an EMS (acute renal syndrome) modeling period, so that the volume and weight of an ectopic focus can be remarkably reduced, local fibrosis, invasion and transfer are inhibited, and the pregnancy rate of an EMS model mouse can be remarkably increased. The leonurine inhibits proliferation and invasion of endometrium stromal cells by inducing apoptosis of the endometrium stromal cells, adjusts expression balance of a local estrogen-progestin receptor of the ectopic focus, activates EMS model pelvic and abdominal immune cells, enhances phagocytic and killing functions of the cells, reverses poor ecdysis of the EMS model mouse, has similar effects on growth and invasion of ectopic focus ESC of a human EMS patient and expression of the estrogen-progestin receptor, does not affect growth and activity of the endometrium ESC of a normal child-bearing female, hormone receptor expression, ecdysis process and proliferation, apoptosis and cell activity of human early-pregnancy embryo trophoblast, and can be used for preparing an effective prevention and treatment medicine for EMS combined infertility patients.

The invention relates to the technical field of biology, in particular to a tissue microarray of decidua and villi pairing, a preparation method and application thereof. The tissue microarray is prepared with the decidua and villus specimens of a normal pregnancy woman or a pathologic pregnancy patient arranged in sample application holes, so that pairing is formed between the decidua tissue and the villus tissue from the same specimen, and the functional presentation of the maternal-fetal interface of a physiological structure onto the tissue microarray is achieved. The tissue microarray has the advantages that high throughput is achieved, plenty of information is obtained in one time, and the efficiency is improved by hundreds of times; parallelization is achieved, the comparability is high, and the precision is high; the experimental error is small, and experimental conditions remain the same; convenience is brought to arrangement of various kinds of experimental control, and the research cost is greatly reduced. The tissue microarray can be used for etiology screening and molecular typing of pathologic pregnancy, screening and verifying of a novel marker, screening of individual treatment patients, judgment of a therapeutic effect and prognosis and the like, and the tissue microarray has important significance for the research of a normal pregnancy maintaining mechanism and pathologic pregnancy pathogenesis.

The invention relates to application of aspirin in preparation of a medicine for preventing and treating unexplained recurrent abortion. According to the invention, a small dose of aspirin is added into the drinking water of the unexplained recurrent abortion mouse, so that the embryo absorption rate is reduced, the inflammatory response of the decidua tissue of the mouse is improved, the HMGB1 level of the serum and the decidua tissue of the mouse is reduced, and the proinflammatory response further caused by the high-level HMGB1 of the unexplained recurrent abortion mouse is prevented; further, the pregnancy rate of the mice with unexplained recurrent abortion is effectively protected and treated.

The invention discloses an application of parasitic loranthus total polysaccharides in preparation of an antiabortifacient. The parasitic loranthus total polysaccharides can promote pregnancy deciduas, and expression of estrogen and progestrone receptor of an ovary of an animal model, and can improve serum E2 and P level; secretion within the gestation period is adjusted; colporrhagia of the animal model is reduced; the abortion frequency is lowered; the abortion rate is lowered; gestation of rats is protected from multiple levels and factors; the miscarriage prevention effect is significant; and the curative effect is more excellent than those of parasitic loranthus total extracts.

The invention discloses a method for obtaining decidua basalis mesenchymal stem cells and a kit. The method for obtaining the decidua basalis mesenchymal stem cells comprises the following steps: carrying out digestion treatment on decidua basalis by utilizing a mixed enzyme preparation, so as to obtain digested products; and separating the decidua basalis mesenchymal stem cells from the digested products, wherein the mixed enzyme preparation comprises pancreatic enzymes, collagenase IV and collagenase II. By adopting the method for obtaining the decidua basalis mesenchymal stem cells, large quantity of decidua basalis mesenchymal stem cells with higher purity and higher activity can be obtained.