Method for preparing decidua mesenchymal stem cell

Abstract

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

Description

technical field [0001] The invention relates to a method for preparing decidual mesenchymal stem cells, belonging to the technical field of stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells derived from the developing mesoderm, which have the potential of self-renewal and multilineage differentiation. Form bone, cartilage, fat, cardiac muscle and other tissues. Mesenchymal stem cells were originally mainly derived from bone marrow, but the content of mesenchymal stem cells (BMMSC) in human bone marrow is extremely low. In recent years, mesenchymal cells with stem / progenitor cell characteristics have been isolated from various tissues such as peripheral blood, dental pulp, cartilage, muscle, umbilical cord, and placenta. Umbilical cord mesenchymal stem cells, amniotic mesenchymal stem cells and chorion mesenchymal stem cells derived from the daughter body can be isolated from the placenta tissue, as well as decidual...

AI Technical Summary

Problems solved by technology

[0005] 1) The existing technology of decidua tissue separation cannot remove the pure decidual tissue from the mother, and the chorionic tissue or amniotic tissue from the daughter that is always mixed with the placenta

[0006] 2) The existing technologies for placenta collection and decidua separation lack effective pollution reduction measures, and the anti-pollution measures have limited effect. Inhibiting the reproduction of contaminating bacteria during the cultivation process cannot effectively reduce pollution;

[0007] 2) The existing enzymatic digestion method is complex and requires multiple enzymatic digestions;

[0008] 3) Operations such as enzyme digestion, cryopreservation and recovery may cause cell

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