Shiitake cultivating and inoculating method

A technology of shiitake mushroom and inoculation room, which is applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of large gap between bacterial strains and bacterial bag medium, low inoculation efficiency, and high pollution probability, saving labor and producing bacteria. The effect of fast speed and low chance of contamination

Inactive Publication Date: 2014-02-05
河北省农业环境保护监测站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages of this method are: high probability of contamination; large gap between bacteria and bacteria bag culture medium, long time for germination and low inoculation efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: a kind of shiitake mushroom cultivation and inoculation method is characterized in that operating according to the following steps:

[0016] 1. Put the mushroom cultivation medium into the bacteria bag, the bacteria bag forms a cylinder, put the bacteria bag into the sterilization room for sterilization and cool to room temperature;

[0017] 2. Move the bacteria bag into the inoculation room for inoculation;

[0018] Three, in the inoculation chamber, punch a rectangular hole axially at the center of the two ends of the cylinder bacteria bag, insert a rectangular mushroom strain in the rectangular hole, and seal it with a tampon or sponge; The length of the mushroom strain longer than the rectangle is 2cm-3cm, the width of the rectangular hole is greater than the width of the rectangular mushroom strain 0.2cm-0.3cm, and the height of the rectangular hole is greater than the height of the rectangular mushroom strain 0.2cm-0.3cm;

[0019] 4. Move the inocul...

Embodiment 2

[0021] Embodiment 2: a kind of shiitake mushroom cultivation and inoculation method is characterized in that it operates as follows:

[0022] 1. Put the mushroom cultivation medium into the bacteria bag, the bacteria bag forms a cylinder, put the bacteria bag into the sterilization room for sterilization and cool to room temperature;

[0023] 2. Move the bacteria bag into the inoculation room for inoculation;

[0024] Three, in the inoculation chamber, punch a rectangular hole axially at the center of the two ends of the cylinder bacteria bag, insert a rectangular mushroom strain in the rectangular hole, and seal it with a tampon or sponge; The length of the mushroom strain longer than the rectangle is 2cm-3cm, the width of the rectangular hole is greater than the width of the rectangular mushroom strain 0.2cm-0.3cm, and the height of the rectangular hole is greater than the height of the rectangular mushroom strain 0.2cm-0.3cm;

[0025] 4. Move the inoculated bacteria bag in...

Embodiment 3

[0027] Embodiment 3: a kind of shiitake mushroom cultivation and inoculation method is characterized in that it operates as follows:

[0028] 1. Put the mushroom cultivation medium into the bacteria bag, the bacteria bag forms a cylinder, put the bacteria bag into the sterilization room for sterilization and cool to room temperature;

[0029] 2. Move the bacteria bag into the inoculation room for inoculation;

[0030] Three, in the inoculation chamber, punch a rectangular hole axially at the center of the two ends of the cylinder bacteria bag, insert a rectangular mushroom strain in the rectangular hole, and seal it with a tampon or sponge; The length of the mushroom strain longer than the rectangle is 2cm-3cm, the width of the rectangular hole is greater than the width of the rectangular mushroom strain 0.2cm-0.3cm, and the height of the rectangular hole is greater than the height of the rectangular mushroom strain 0.2cm-0.3cm;

[0031] 4. Move the inoculated bacteria bag in...

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PUM

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Abstract

The invention discloses a shiitake cultivating and inoculating method and belongs to the technical field of mushroom cultivation. The shiitake cultivating and inoculating method is characterized by comprising the following steps of: 1, filling a shiitake cultivating culture medium in fungus bags, enabling the fungus bags to be cylindrical, placing the fungus bags in a sterilization room for sterilization, and cooling to a room temperature; 2, moving the fungus bags in an inoculation room for inoculation; 3, forming rectangular holes in the axial direction of the centers of the two end faces of each cylindrical fungus bag in the inoculation room, inserting shiitake strains with rectangular sections in the rectangular holes, and sealing the holes by using cotton plugs or sponge, wherein the length of each rectangular hole is 2 cm to 3 cm greater than that of each rectangular shiitake strain, the width of each rectangular hole is 0.2 cm to 0.3 cm greater than that of each rectangular shiitake strain, and the height of each rectangular hole is 0.2 cm to 0.3 cm greater than that of each rectangular shiitake strain; 4, moving the inoculated fungus bags in a fungus culturing room for mycelium cultivation. According to the invention, contact space between the strains and the culture mediums in the fungus bags is suitable, the fungus growing speed is fast, and the inoculation efficiency is high.

Description

technical field [0001] The invention relates to the technical field of mushroom cultivation. Background technique [0002] The existing shiitake mushroom cultivation and inoculation method is to put the culture medium into the bacteria bag, put it into the sterilization room for sterilization, and then move the bacteria bag into the cooling room. 3-4 equidistant circular holes with a diameter of 1.5cm and a depth of 6cm are manually punched on the surface of the bacteria bag, and the wood chip bacteria are broken into pieces by hand and stuffed into each hole and pressed tightly, and then bagged or Seal the inoculation hole with adhesive tape, and after the inoculation is completed, move the bacteria bag into the culture room for mycelium cultivation. The disadvantages of this method are: the probability of contamination is high; the gap between the strain and the bacteria bag culture medium is large, the time for germination is long, and the inoculation efficiency is low. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 通占元李冬梅
Owner 河北省农业环境保护监测站
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