703results about How to "Reduced Chances of Contamination" patented technology

The invention provides self-disinfection chicken cage culture equipment. The self-disinfection chicken cage culture equipment comprises a frame, a cage and an egg conveying belt. A limiting plate is fixed to the upper portion of a front panel of the cage, the limiting plate is connected with an upper side plate in a sliding mode, hydraulic rods are fixed to the upper portion of a rear side plate, movable frames are fixed to the output ends of the hydraulic rods and fixedly connected with the limiting plate, a liquid inflow pipe is arranged on the upper portions of the side plates, and a medicine kettle is connected to the liquid inflow pipe in a threaded mode. Disinfection medicine is contained in the medicine kettle and comprises 60-90 g of folium artemisiae argyi, 60-90 g of sanguisorba officinalis, 60-90 g of gentiana scabra, 30-50 g of Rhizoma Ligustici, 30-50 g of cimicifugae foetidae, 30-50 g of cortex phellodendri, 30-50 g of rhizoma anemarrhenae, 30-50 g of curcuma zedoary, 30-50 g of radix sophorae flavescentis, 15-30 g of tripterygium glycosides, 15-30 g of Chinese azalea flowers, 15-30 g of calamine, 15-30 g of tenuifolia, 15-30 g of aloes, 15-30 g of ash bark, 15-30 g of radix scrophulariae, 10-20 g of chrysanthemums, 15 g of hydrargyrum oxydatum crudum and 15 g of peracetic acid. The self-disinfection chicken cage culture equipment is simple in structure, convenient to use and good in chicken output effect, the bodies of the chickens and the emptied cage are synchronously disinfected in the chicken output process, and the pathogenic bacterium contamination probability is reduced.

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

The invention relates to a method for producing lactic acid drink, which comprises that 1, preparing milk phase; 2, adding carbon dioxide gas; 3, packing, disinfecting to obtain the lactic acid drink. Compared with general method, the invention can reduce the pollution of microbe, improve the quality and widen the application.

In order to solve various performance and security problems like querying delay, update delay, and vulnerability to DoS attack and the like in the existing domain name parse system, the invention brings forward a novel domain name parse service model that is capable of realize incremental deployment, is compatible with the existing DNS, and has the good performance. To be specific, the invention relates to a content-distribution-network (CDN)-based domain name parse service model. The core concept of the model design is as follows: CDN network node server systems that are distributed widely form a system. On the basis of the content distribution and storage mechanism of the CDN network, authoritative DNS records all domain names are distributed at the node servers that are distributed widely by a pushing way. The DNS records are distributed by using the CDN architecture. The authoritative DNS records are distributed to all node servers; all node servers replace a domain name parse system and a domain name server in the existing DNS architecture to response to a domain name parse request of a user and provide a domain name parse service; and the query result is returned to the user directly by a network node server.

The invention provides a thymopentin injection, comprising a thymopentin as an active component, a plurality of accessories for injection, and certain amount of sterile water for injection, wherein the accessories for injection comprises protective agent, antioxidant and pH regulator. The dosage is as follows: point one to two hundred mg/ml thymopentin, ten to two hundred mg/ml protective agent, point one to ten mg/ml antioxidant, and point five to twenty mg/ml pH regulator. The injection has the advantages of stability while refrigerated at the temperature between two to ten degrees centigrade and long periods of preservation; the purity of the thymopentine remains ninety-nine percent after being stored for twenty-four months; the injection can be stored for six months to twelve months at normal temperature, which significantly facilitates circulation and transportation of drugs, thus ensures excellent stability of the injection.

The invention provides a liquid milk tea and a preparation method thereof. 100 percent of the weight of the liquid milk tea is used as benchmark; the liquid milk tea comprises the following compositions in weight percentage: 10 to 50 percent of milk, 25 to 30 percent of tea soup, 0.1 to 1 percent of a stabilizer, 0.01 to 8 percent of a sweetening agent, 0.01 to 0.2 percent of buffer salt and 0.01 to 0.1 percent of an antioxidant, wherein 25 to 30 percent of the tea soup is a tea extracting solution which is obtained through leaching 0.5 to 1.5 percent of dried tea leaves in hot water. The liquid milk tea has the characteristics that the liquid milk tea contains the health-care compositions of the tea, remains the natural nutritious compositions of the milk, is a liquid beverage with smooth mouthfeel and fragrant and rich scent, minimizes the composition loss of a dairy product and a tea beverage and keeps the natural color of freshness, fragrance and richness.

A totally-integrated high-flux cell level micro-fluidic chip medicine evaluating system is a multifunctional cell level medicine evaluating system treating a micro-fluidic chip as a core technology, starting from the total design of an integrated high-flux automatic micro-fluidic chip system and integrating a cell function chip module, a flow path control module, a cell culture microenvironment module and a cell response signal real-time monitoring module. The totally-integrated high-flux cell level micro-fluidic chip medicine evaluating system has the characteristics of total integration, high flux, less sample consumption, totally-closed operation, realization of multi-parameter real time monitoring, and the like, has a very strong original innovativeness, and is of great significance to the large-scale medicine research and development.

The invention discloses a wall-hung thin type lampblack depurating machine, which is provided with flat cuboid-shaped shell, wherein a double-volute is arranged in the shell, and two isolated chambers of the double-volute share an air outlet passage on the top of the shell; a turbo-fan with a horizontal output shaft is arranged in the two isolated chambers of the double-volute respectively, and the front end of the output shaft of the turbo-fan extends out of the double-volute and is connected with at least one vertical rotary centrifugal filter screen plate; and the outside of the rotary centrifugal filter screen plate is provided with an annular oil guide ring, the bottom of the annular oil guide ring is provided with an oil leak opening, a fume flow distribution plate is arranged below the annular oil guide ring, an oil guide tank is arranged on the fume flow distribution plate, and the bottom of the shell is provided with an air inlet passage and an oil collection box. The wall-hung thin type lampblack depurating machine has ultra-high efficiency for holding up and collecting lampblack, can basically put an end to the pollution caused by the lampblack to the air, the shell and the external wall of a building, and has the characteristics of overall closure, simple structure, convenient cleaning, energy saving and noise reduction, large cooking space, safe running and long service life.

The invention provides a solid tumor patient autologous NK cell separation, excitation, amplification and activity detection method. By the adoption of the NK cell separation, culturing and amplification method, highly active lymphocyte populations can be obtained through separation from peripheral blood, through stimulation of a series of in-vitro cell factors, NK cells (CD3-CD56+) are purified, activated and amplified greatly, and the NK cells can be applied to clinic treatment of tumors. Through the NK cell activity detection method, the kill rate of effector cells to target cells can be specifically analyzed in mixed cell sap of immune cells and tumor cells, interference from the effector cells in a detection result is avoided, and early apoptosis target cells and late apoptosis target cells can be analyzed. The method is easy and convenient to implement, the flexibility is high, the kill function of a cell can be detected at the single cell level, and the method has important significance in fundamental research conducted before clinical application of tumor adoptive cellular immunotherapy and quality control conducted in the clinical application process.

The invention discloses a human ALK fusion gene detection primer set and a detection kit. The primer set comprises one or more detection primers in sixteen ALK fusion gene types A01 to A16 and Taqman probes corresponding to the gene types. As many as sixteen fusion gene types except for EML4 can be detected through the detection kit formed by the primer set and the probes, the requirement for the sample RNA obtaining quality is low, and the ideal detection effect can be achieved through samples in different existence forms; by means of the kit, an operator only needs to directly feed extracted sample RNA once, inverse transcription and PCR amplification are carried out in one closed tube reaction system, and the pollution probability and the result error probability are reduced. One-time detection only takes eighty minutes. The human ALK fusion gene detection primer set and the detection kit have the remarkable advantages that the number of the detection types is large, sensitivity is high, experiment operation is simple, the period is short, the human ALK fusion gene detection primer set and the detection kit are safe and nontoxic, and cost is low.

A method for forming a side wall layer on a grid electrode comprises the following steps: a semi-conductor substrate provided with the grid electrode is provided; a first silica layer is formed on the surfaces of the semi-conductor substrate and the grid electrode; with hexa-chloro silane as a precursor, a first silicon nitride layer is formed on top of the first silica layer; and parts of the first silica layer and the first silicon nitride layer are removed in a selective manner so as to preserve the first silica layer and the first silicon nitride layer already formed on the side wall of the grid electrode. The invention further provides a method for manufacturing a semiconductor device. The invention can solve the problem that the bottom part of the side wall layer on the grid electrode is susceptible to depression.

The invention discloses a processing method of oil sludge. The method comprises steps that: (1) the oil sludge is dehydrated through supersonic wave pretreatment, such that the water content in the oil sludge is reduced; (2) under an anoxybiotic condition, oil sludge processed through supersonic wave dehydration is heated to a temperature in a range above a boiling point of water an below a hydrocarbon substance cracking temperature; the oil sludge is delivered into a separating tower, and is subject to flash evaporation; light oil and water are recovered with an evaporation and condensation manner, and heavy oil and solid substances are fetched from the separating tower with a form of slurry; (3) the slurry obtained from the tower bottom is subject to solid-liquid separation; the heavy oil is recovered, and oil in the solid substances is further recovered through steam distillation. With the method provided by the invention, various valuable components in the oil sludge can be fully recovered. The method provides good economical efficiency in industrial applications.

A method for preparing chondroblast of bone marrow mesogalia stem cell with three dimensional cultivation and induction includes steps of placing bone marrow mesogalia stem cell into suspending culture device preset with culture medium and microcarrier for suspending cultivation; cloning abovesaid stem cells with agitation, adding induction culture medium of chondroblast in culture meidum containing harvested cytodex3 microcrrier and abovesaid stem cells gromn on it; and carrying out induction for chondroblast assembly from abovesaid stem cells.

The invention discloses a spherical granule bacteria cellulose and preparing method thereof and special culture medium. The culture medium provided by the invention comprises carbon, nitrogen source, inorganic salt and growth factor, also comprises coconut water with percentage by volume 0%-80% and carbon source concentration 1-30g/L. The preparing method of spherical bacteria cellulose provided by the invention is to place the production bacterium of bacteria cellulose in the culture medium to oscillate for fermenting or in the fermentation cylinder to ventilate for cultivating. The spherical bacteria cellulose of the invention can be directly applied to the food processing without the cutting operations. The oscillation fermentation needs less nutrition, and is suitable for fermentation cylinder ventilated cultivation, the pollution probability is small, the product yield rate is high, the cellulose product is not traditional film sheets-shaped, and is a bran-new spherical granule-shaped, stabbed spherical or star flower-shaped, the harvesting and the rinsing are easy, especially without extra cutting operations, the spherical granule sizes can be regulated according to the fermentation condition and time.

The invention provides Puer milk-tea powder. The Puer milk-tea powder comprises dry mixture of the extracts of Puer and milk. The dry mixture is made from Puer tea juice and liquid milk by mixing, homogenization, concentration and drying, wherein, the Puer tea juice is made by mixing 200 to 300 weight portions of dry Puer tea and water, the quantity of the water is at least one times of the dry Puer tea, and decocting at least half an hour, and the liquid milk holds 4300 to 7880 weight portions. The invention has the advantages that the Puer milk-tea powder is not only rich in protein, fat and calcium, but also contains amino acid and trace element supplying for human body, and can rapidly recover physical fitness, relieve fatigue, refresh and reduce fat and weight.

The invention discloses a shelling and integral kernel extracting machine for kiskatom, which comprises a stand (2), a feeding hopper (13), a discharging hopper (20), a motor (1) and a driving device of the motor (1). The shelling and integral kernel extracting machine is sequentially provided with the feeding hopper (13) from top to bottom; a kick-off grid (17) is arranged in the feeding hopper (17); a fruit falling pipe (18) is arranged at the bottom of the feeding hopper; an elastic impacting device and a fruit falling control device which are horizontally arranged are arranged below the feeding hopper (13); the discharging hopper (20), a transverse shaft (22) and a volute piece (21) on the transverse shaft are arranged below the elastic impacting device and the fruit falling control device; a gearbox of the motor (1) and a driving device are arranged at the lowermost part of the elastic impacting device and the fruit falling control device; and the elastic impacting device consists of a pressure spring (8), a socket spring rod (9), a guide sleeve (10), a sinking bar (15) and a fruit mortar (19). The invention relates to the shelling and kernel extracting machine and has high production efficiency and less pollution to the kernel compared with manual shell knocking and kernel extracting; and compared with a traditional shelling machine, the shelling and integral kernel extracting machine has high complete rate of integral kernel extraction of the kiskatom and improved selling price of the kernel.

The invention belongs to the gene detection field, and provides a complete set primer for detecting a HIV-1 drug-resistant mutation site. The complete set primer for detecting the HIV-1 drug-resistantmutation site comprises nucleotide sequences shown from SEQ ID No.1 to SEQ ID No.5 in a sequence table. The invention further provides an application of the complete set primer for detecting the HIV-1 drug-resistant mutation site. The complete set primer can amplify more than 1000 copy/ml of HIV-1 po1 zone gene sequence by one PCR reaction only, thus the work intensity is reduced and the pollution ratio is reduced; meanwhile, the scale of the drug-resistant mutation site covered by the amplified segment is wide, and the complete set primer is applicable to main HIV-1 prevailing subtype drug-resistant mutation site detection at home.

The invention discloses a portable loader for a micropipette tip box. The portable loader comprises a box body, and an upper-layer plate, a fan and a lower-layer plate which are arranged in the box body, wherein the top of the box body is provided with an openable and closable tip inlet, the upper-layer plate is fixed below the tip inlet, the lower-layer plate is arranged below the upper-layer plate, and the fan is fixedly arranged above the lower-layer plate on the box body. The portable loader adopts a simple physical principle, and tips are arranged in transverse slotted holes of the lower-layer plate of the loader by setting the wind speed of the fan, so that the loading efficiency of tips used in a laboratory can be greatly improved, and the labor intensity of operators is reduced by convenient and quick loading. The contamination probability of the tips can be reduced by virtue of the loader. The loader has a simple structure, is easy to process, has a small size, is convenient to operate, can save a space for the laboratory, is also suitable for loading of other tips with various sizes, and can be widely popularized and used.

The invention discloses primers, a probe and a kit for detecting human MET gene 14 exon splicing mutation. The invention provides a specific primer pair A. The specific primer pair A is composed of a primer 1 and a primer 2 which are respectively disclosed as Sequence 3 and Sequence 4 in the sequence table. The invention also provides a primer-probe combination A which is composed of the specific primer pair A and a probe A, wherein the nucleotide sequence of the probe A is disclosed as Sequence 5 in the sequence table. The invention also provides a kit B which comprises the primer-probe combination A. The invention also provides application of the primer-probe combination A or the kit B in detecting whether mutation for causing MET gene 14 exon jump deletion occurs in the human genome DNA (deoxyribonucleic acid). The method is simple to operate, has the advantages of short detection time, high sensitivity, high specificity, high safety, no toxicity, low cost and the like, and can satisfy the actual demands for clinical quick detection.

The present invention discloses a method for cultivating the mesenchymal stem cells from the oral tissue. A dental pulp or gingival mucosa sample is prepared, dental pulp mesenchymal stem cells are abstracted from the dental pulp tissue of the tooth or gingival mucosa mesenchymal stem cells are abstracted from a gingival mucosa tissue, then, the cells are cultivated, the cells present the kenel of the dental pulp mesenchymal stem cells or kenel of the gingival mucosa mesenchymal stem cells, and the next cultivation and the preservation are operated. The dental pulp mesenchymal stem cells and the gingival mucosa mesenchymal stem cells abstracted through the method of the present invention have the abilities of clustering, differentiating into the substance of bone, differentiating into fat, and differentiating into nerve. The method of the present invention reduces the pollution rate, and leads the mesenchymal stem cells to be easily cultivated and preserved.

The invention relates to a method for pickling salted eggs in a pressure circulation manner. The method comprises the following steps of: cleaning and airing fresh eggs after being tested; sorting, airing, and transferring the eggs into a pickling turnover box; putting the eggs into a sealed pressure tank, adding a saturated salt solution, and beginning to pickle in the pressure circulation manner, wherein the pickling in the pressure circulation comprises the steps of: circularly changing the air pressure inside the tank between constant pressure of 0.1Mpa and the pressure of 0.15Mpa; firstly, pressurizing to be up to 0.15Mpa and maintaining for 10min; and decompressing to be up to the constant pressure of 0.1Mpa for 2-3min, and then maintaining the constant pressure for 20min, so as to finish a regular cycle. The method disclosed by the invention has the advantages of simplicity and reliability; the conventional formulation, and new pressure processing devices and technologies are adopted; the original devices in a factory are fully utilized; a production line with automatic operation can be formed; the pickling period is shortened from 15-40 days to 3-4 days; the product quality is ensured; the dispensing quantity of salt is reduced; picking feed liquid can be reused for 5-6 times after fabrication treatment; and the salt can be saved, and the environmental pollution is also avoided.

The invention discloses a physical sewage treatment system used for sewage treatment from the source, comprising a solid-liquid separation device and a settling device. The solid-liquid separation device is provided with a solid-liquid separator, and a separation water inlet, a separation water outlet and a separation sewage discharging port of the solid-liquid separator; the settling device comprises a settling shell the bottom of which is provided with a settling sewage discharging port, a water inlet hose and a water outlet hose are arranged in the settling shell, the water inlet end of the water inlet hose is connected with the separation water outlet of the solid-liquid separation device, the water outlet end of the water inlet hose is connected with a hollow floating disk, the outline of the floating disk adapts to that of the cross section of the settling shell, and a plurality of through holes are uniformly distributed in the annular area of the bottom surface, close to the edge; a floating disk water outlet is arranged on the bottom of the floating disk and is connected with the water inlet end of the water outlet hose, and the water outlet end of the water outlet hose extends out of the settling shell. The sewage treatment system can be used for treating sewage timely from the pollution sources, thus reducing or avoiding the phenomenon that sewage enters public water body.

This invention relates to a method for preparing fermentation culture medium and producing biological pesticide from fermentation wastewater. The method comprises: (1) utilizing fermentation liquid, ethanol wastewater and monosodium glutamate wastewater as the raw materials, and Streptomyces griseus as the strain; (2) fermenting at 28 deg.C and 100-150 rpm for 7-10 days; (3) filtering, concentrating and purifying to obtain biological pesticide. The method utilizes fermentation wastewater to prepare fermentation culture medium and produce biological pesticide, thus can effectively combine the treatment of fermentation wastewater and utilization of useful resources in fermentation wastewater. The treatment rate is high and the cost is low. Besides, the method for produing biological pesticide has such advantages as simple process, low cost, high productivity, and low probability of culture medium pollution and good antibacterial effect of the biological pesticide.