Spherical granule bacteria cellulose and preparing method thereof and special culture medium

A technology of bacterial cellulose and spherical particles, applied in biochemical equipment and methods, methods based on microorganisms, fermentation, etc., can solve the problems of gel cellulose without spherical particles, achieve novel and unique appearance, save man-hours, and produce products The effect of high yield

Inactive Publication Date: 2009-11-25
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the special production of spherical particle gel cellulose

Method used

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  • Spherical granule bacteria cellulose and preparing method thereof and special culture medium
  • Spherical granule bacteria cellulose and preparing method thereof and special culture medium
  • Spherical granule bacteria cellulose and preparing method thereof and special culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of spherical particle bacterial cellulose

[0044] 1. Determination of the optimal components and content of the fermentation medium

[0045] The preserved strain CGMCC1.1812 on the slant medium was activated for 1-2 generations on the fresh slant medium. Since the bacterial cells are easily wrapped by cellulose products when cultured on a slant, the lawn sticks into sheets, so it is advisable to streak inoculate intermittently when transferring to a slope, and do not streak continuously to prevent the lawn from sticking and making it difficult to transfer. Then insert a small piece of bacterial lawn into 20ml liquid seed culture medium (100ml Erlenmeyer flask, sealed with air-permeable sealing film), vibrate and culture at 30°C and 120r / min for 20-24h to make seed culture solution.

[0046] Then the seed liquid is inserted into the liquid fermentation medium by 4%-5% (to avoid inserting fiber filaments) and carried out various condition t...

Embodiment 2

[0081] Embodiment 2, the preparation of spherical particle bacterial cellulose

[0082] The difference between this example and Example 1 is that the bacterial strain used was replaced by the bacterial strain accc10215, and the rest of the test conditions were completely the same.

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Abstract

The invention discloses a spherical granule bacteria cellulose and preparing method thereof and special culture medium. The culture medium provided by the invention comprises carbon, nitrogen source, inorganic salt and growth factor, also comprises coconut water with percentage by volume 0%-80% and carbon source concentration 1-30g/L. The preparing method of spherical bacteria cellulose provided by the invention is to place the production bacterium of bacteria cellulose in the culture medium to oscillate for fermenting or in the fermentation cylinder to ventilate for cultivating. The spherical bacteria cellulose of the invention can be directly applied to the food processing without the cutting operations. The oscillation fermentation needs less nutrition, and is suitable for fermentation cylinder ventilated cultivation, the pollution probability is small, the product yield rate is high, the cellulose product is not traditional film sheets-shaped, and is a bran-new spherical granule-shaped, stabbed spherical or star flower-shaped, the harvesting and the rinsing are easy, especially without extra cutting operations, the spherical granule sizes can be regulated according to the fermentation condition and time.

Description

technical field [0001] The invention relates to the field of food industry, in particular to spherical particle bacterial cellulose, a preparation method thereof and a special culture medium. Background technique [0002] Bacterial cellulose has a wide range of commercial applications due to its extremely high purity and unique properties. In particular, it has high water holding capacity, high crystallinity, high elastic modulus, high colloidal strength, and low solid content, so it is often used as a filler, dispersant, stabilizer, and thickener in the food industry , excipients and texture modifiers, etc. (Kongruang S. Bacterial cellulose production by Acetobacter xylinum strains from agricultural waste products [J]. Applied Biochemistry and Biotechnology, 2008, 148(1-3): 245-56.). Because its main component is cellulose, it is also recognized as a safe and reliable GRAS grade (Kent RA, Stephens RS, Westland JA. Bacterial cellulose fiber provides an alternative for thick...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08L1/02C12R1/02C12R1/01
Inventor 刘四新李从发
Owner HAINAN UNIVERSITY
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