307 results about "Fermentation condition" patented technology

The invention discloses an intelligent regulation and control method for a tea fermentation process and a regulation and control system based on microbial activity monitoring, and relates to the technical field of tea processing. According to the method, microbial activity and environmental parameters are monitored in real time, and a microbial metabolism model and a neural network algorithm are combined, so that dynamic optimization and accurate regulation and control of the tea leaf fermentation process are realized, firstly, microbial activity data and environmental parameters in a fermentation environment are collected by utilizing a sensor array; the method comprises the following steps: firstly, calculating a metabolite generation rate and a concentration change trend through a microbial metabolism model, accurately identifying abnormal fluctuation in a fermentation process, secondly, adjusting fermentation conditions in real time based on the metabolite concentration change trend and a dynamic regulation and control algorithm to ensure the stability of the fermentation process, and finally, carrying out real-time fermentation. Tea quality parameters after fermentation condition adjustment are predicted through a neural network algorithm, a regulation and control strategy is optimized, and stable improvement of tea quality is achieved.

The invention discloses an Escherichia coli mutant strain which is classified and named as Escherichia coli, the strain number is CR-TK01, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35554. The invention further discloses a preparation method of the Escherichia coli mutant strain. The Escherichia coli CR-TK01 with high yield of cytidine provided by the invention has the characteristics of high yield and stable high-yield character, glucose is used as a carbon source, the Escherichia coli CR-TK01 is used for fermentation production of cytidine under a certain fermentation condition, and the yield of cytidine is as high as 97.23 g / L; the strain is not degraded after passage for more than 20 generations, the performance of high-yield cytidine is maintained in the passage fermentation process, and the yield of cytidine in passage fermentation is improved. The method for producing cytidine through fermentation is simple in process, high in yield and suitable for industrialization.

The invention provides a method for promoting generation of tea acid from Nemania sp YAFEF112, and relates to the technical field of microbial culture. According to the method, mycelia of Nemania sp YAFEF112 are inoculated into a culture medium containing pruned walnut branches for culture, the culture medium is an MM culture medium, and the specific formula of the culture medium comprises 1.52 g / L of monopotassium phosphate, 0.52 g / L of magnesium sulfate, 6 g / L of sodium nitrate and 0.52 g / L of potassium chloride. According to the method, the defects in the prior art are overcome, and good conditions are provided for the production of the tea pickled acid by optimizing the formula of the fermentation culture medium, adding pruned walnut branches into the culture medium, improving the fermentation conditions and increasing the content of the tea pickled acid in the Nemania sp YAFEF112 hyphae.

The invention belongs to the field of microorganisms, and particularly relates to bacillus Z161 capable of efficiently degrading straw as well as a screening method and application of the bacillus Z161. The bacillus subtilis Z161 is bacillus mexicanus, the preservation number of the bacillus mexicanus Z161 is CGMCC (China General Microbiological Culture Collection Center) NO.36393, and the bacillus mexicanus Z161 is preserved in the China General Microbiological Culture Collection Center on October 29, 2025. The bacillus Z161 screened by the invention has strong laccase activity and high temperature resistance, and can efficiently degrade lignin under the condition of 50 DEG C; the problem that in the prior art, normal-temperature zymophyte is mostly adopted, and the requirement for fermentation conditions in the fermentation process is harsh is solved, and therefore the rapid compost fermentation technology is easier to achieve. The bacillus Z161 provided by the invention has the advantages of genetic and functional character stability, has good tolerance at high temperature, enables lignin to be quickly depolymerized, and improves the decomposition efficiency.

The invention discloses a high-yield flower and fruit aroma strain, culture thereof and application of the high-yield flower and fruit aroma strain in sauce-flavor food brewing, the strain is named as Wickerhamomyces anomalus GZZJ2023Y053, and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 2025875, and the preservation number of the strain is CCTCC NO: M 2025875. The preservation date is April 24, 2025; the preservation address is Wuhan University, Wuhan, China. The culture method of Wickerhamomyces anomalus comprises the following steps: inoculating activated Wickerhamomyces anomalus GZZJ2023Y053 into a liquid culture medium, and fermenting at constant temperature to obtain a seed solution; the invention also provides an application of the Wickerhamomyces anomalus GZZJ2023Y053 in preparation of ethyl acetate and brewing of sauce-flavor foods. According to the method, multiple Wickerhamomyces anomalus separated from fermented grains is subjected to separation culture, the content of ethyl acetate is measured after fermentation is finished, then the screened Wickerhamomyces anomalus GZZJ2023Y053 has the capacity of producing ethyl acetate at high yield, and after fermentation conditions are further optimized, the yield of the ethyl acetate is improved. The capability of synthesizing ethyl acetate is greatly improved.

The invention discloses a recombinant engineering bacterium for expressing fetuB and application of the recombinant engineering bacterium, and relates to the technical field of genetic engineering. According to the recombinant engineering bacteria, pichia pastoris serves as host bacteria, after fetuB genes are introduced, efficient expression of the fetuB in the pichia pastoris is achieved by optimizing codons and improving fermentation conditions, and the expression quantities of CBS7435 under the shake-flask culture condition are 320 + / -25 mg / L, SMD116 under the shake-flask culture condition is 280 + / -30 mg / L, and SuperMan5 under the shake-flask culture condition is 580 + / -45 mg / L. After fermentation conditions are optimized, the culture yield of a fermentation tank can reach 27.35 g / L. The fetuin B disclosed by the invention is good in biological activity, high in expression, simple in operation process and suitable for large-scale industrial production.

The invention discloses recombinant escherichia coli with high yield of N-acetylneuraminic acid and application of the recombinant escherichia coli, and relates to the technical field of biological genetic engineering. The invention relates to a recombinant escherichia coli, which is characterized in that the escherichia coli is taken as a host, and free expression of an N-acetylmannosamine epimerase gene yihS from Streptomyces xiamenensis or an N-acetylmannosamine epimerase gene ce3 from Bacteroides polymorpha and an exogenous N-acetylneuraminic acid lyase gene nano A is carried out; and carrying out recombinant expression on N-acetyl hexosamine 1-kinase nahK, a UDP-N-acetyl glucosamine pyrophosphorylase gene glmU and a UDP-N-acetyl glucosamine-2-epimerase gene neuC in the other synthetic route of the ManNAc. According to the recombinant escherichia coli with high yield of N-acetylneuraminic acid, the yield of N-acetylneuraminic acid can reach 23.08 g / L under a shake flask fermentation condition; a two-stage batch feeding strategy is adopted, the yield of N-acetylneuraminic acid in a 5L fermentation tank reaches 71.25 g / L, the molar conversion rate of GlcNAc reaches up to 57.60%, and the method has the potential of industrial application.

The invention belongs to the technical field of microbial culture, and particularly relates to a culture medium composition and application thereof in PDRN recombinant escherichia coli fermentation. The culture medium composition comprises a fermentation culture medium and a fed-batch culture medium, the fermentation culture medium consists of disodium hydrogen phosphate, monopotassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate tetrahydrate, glycerol and water; the supplemented culture medium is composed of dipotassium phosphate, sodium dihydrogen phosphate, sodium sulfate, ammonium sulfate, ammonium chloride, diammonium hydrogen citrate, glycerin and water, and the metabolic requirements of PDRN recombinant escherichia coli are accurately matched. Meanwhile, the invention provides a fermentation method using the composition, and the problems of unbalanced nutrition, low fermentation efficiency and the like of an existing culture medium are solved. Stable proliferation of thalli can be remarkably promoted, the yield of PDRN products is increased, the culture medium is easy and convenient to prepare, fermentation conditions are easy to control, and the culture medium is suitable for industrial large-scale production and has important application value.

The invention provides a uniform fermentation type microbial fermentation device for food processing, and relates to the technical field of food processing. The uniform fermentation type microbial fermentation device comprises a supporting bottom plate, a supporting rod, a connecting flange, a supporting flange, a fermentation tank body, a sealing cover, a supporting sleeve, a movable lifting assembly, a transmission rack, a driving gear, a driven gear, a rotating shaft, a rotating push rod, a cap pressing disc, a detection connector and the like. According to the invention, the movable lifting assembly drives the pressing cap to spirally turn and move downwards, and floating objects on the upper layer of fermentation liquor are pressed into the fermentation liquor, so that the floating objects are in full contact with the fermentation liquor, and the fermentation efficiency and uniformity are improved; the fermentation tank body is made of a tempered glass material, so that the internal fermentation condition is convenient to observe; a buffer pad is arranged between the connecting flange and the supporting flange, so that vibration in the fermentation process is reduced; through the design of a circulating water connector and a circulating water pipe, the temperature in the fermentation tank body can be adjusted.

The invention discloses a scalp care solution with the effects of controlling oil, relieving, inhibiting bacteria and adjusting scalp micro-ecology and a preparation method and application thereof.The preparation method of the scalp care solution comprises the following steps that a fermentation substrate is inoculated with saccharomycetes, primary fermentation culture is conducted for 24-72 h, then secondary fermentation culture is conducted through Lactobacillus gasseri, and the scalp care solution is obtained; wherein the fermentation substrate comprises rice, barley seeds and water; wherein the saccharomycetes comprise Saccharomyces cerevisiae (Saccharomyces cerevisiae) and Saccharomyces fibulligera (Saccharomyces fibulligera), and the saccharomycetes comprise Saccharomyces cerevisiae (Saccharomyces fibulligera) and Saccharomyces fibulligera (Saccharomyces fibulligera). The rice and the barley seeds are fermented in a microbial fermentation mode, the bioavailability of the rice and the barley seeds is improved, the content of active ingredients in the fermentation liquor prepared through the fermentation technology is increased, the fermentation liquor has the ideal effects of controlling oil, resisting inflammation, inhibiting bacteria, adjusting scalp micro-ecology and the like, the preparation technology is simple, and the method is suitable for industrial production. The fermentation conditions are mild, no organic solvent is used, and safety is achieved.

The application discloses a high-precision intelligent control and adjustment method for stachydrine fermentation and purification, and relates to the field of biological fermentation process control. The method comprises the following steps: obtaining a stachydrine mixed solution in a first sugar content interval; inoculating Eurotium cristatum at a first preset temperature after sterilization to start fermentation and obtain an initial fermentation liquor; monitoring the content ratio change of stachydrine and non-target sugars in real time through HPLC to obtain sugar content monitoring data; introducing a membrane separation device for selective dialysis to obtain membrane permeation rate monitoring data; performing dynamic evaluation on stachydrine enrichment according to the sugar content monitoring data and the membrane permeation rate monitoring data, generating a dynamic adjustment strategy for fermentation conditions based on the evaluation results; and performing fermentation process adjustment control. The method solves the technical problem that the existing stachydrine fermentation and purification cannot realize real-time and accurate monitoring and dynamic adjustment of fermentation conditions, resulting in low fermentation efficiency and low purity, and achieves the technical effect of improving the fermentation efficiency and purity of stachydrine through intelligent control of the fermentation process.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention discloses a high-yield caproic acid bacterium and application thereof in production of caproic acid by anaerobic fermentation of white spirit brewing yellow water. According to the invention, a high-yield caproic acid bacterium, namely bacillus aryabhattai H1 (CCTCC NO: M 20241040), is obtained through separation and screening, the high-yield caproic acid bacterium can grow and synthesize caproic acid by independently taking ethanol or lactic acid as a substrate, and the caproic acid production efficiency is optimal when ethanol and lactic acid are taken as a composite substrate. According to the bacillus aryabhattai H1, under the open type fermentation condition that a substrate does not need to be sterilized, the caproic acid can be efficiently produced by taking the white spirit brewing byproduct yellow water as the substrate, and the yield, the yield and the production intensity of the caproic acid can reach 12.5 g / L, 2.5 kg / m < 3 > / d and 22% or above respectively.

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

The invention relates to the technical field of microorganism co-culture fermentation, and discloses a method for producing L-carnitine soybean meal by applying a neural network to optimize fermentation conditions, and the method comprises the following steps: establishing a double-bacterium single-culture control group, collecting a double-bacterium dual-wavelength response data set, and constructing a two-dimensional geometric feature space to obtain a strain feature fingerprint slope; establishing a double-bacterium co-culture fermentation group, decomposing the real-time mixed optical density value to obtain double-bacterium estimated concentration, and constructing an enhanced input data set; then building a bimodal prediction model to output a predicted value of the L-carnitine content; and finally, setting an optimization objective function containing a yield deviation item, a phase regression item and a control smoothing item, and solving to obtain an optimal control input sequence. According to the invention, precise decoupling of the growth state of double bacteria and metabolic phase cooperative regulation and control are realized, information dimension limitation of traditional mixed optical density signals is broken through, and time sequence matching and yield stability of L-carnitine synthesis are guaranteed.

The utility model relates to an automatic turnover device of a black tea fermentation machine, which belongs to the technical field of black tea production and processing and comprises a support frame, a processing cylinder fixedly mounted on the top side of the support frame and a fermentation cylinder fixedly mounted in the processing cylinder, and a turnover mechanism and a watering mechanism are arranged outside the processing cylinder; the turnover mechanism comprises a rotating rod which is rotationally mounted on one side of the processing cylinder and extends into the fermentation cylinder, a stirring plate fixedly welded to the outer surface of the rotating rod, and a driving motor fixedly mounted on the outer wall of one side of the processing cylinder; a plurality of water spraying holes which are formed at equal intervals are formed in the rotating rod; a linkage structure is arranged between the driven gear and the piston pump. According to the automatic turnover device of the black tea fermentation machine, the rotating rod rotates to drive the stirring plate to continuously stir tea leaves in the turnover process, so that the tea leaves are in more sufficient contact with air and fermentation conditions, water can be uniformly sprayed on the tea leaves due to the design of the water spraying holes, and mixing and fermentation of the tea leaves are further promoted.

According to the fermented wine rich in alcohol and sweetness and the preparation process thereof, high-sugar-degree grain liquid (wort) is adopted as a fermentation substrate, fermentation is controlled through functional strains in a labor division and staged mode, and the technical problems that in a traditional process, glycerol synthesis is limited, and a wine body is thin, sour and astringent are effectively solved. The content of glycerol is increased under the pure fermentation condition, meanwhile, the content of by-products such as residual sugar and acetic acid is reduced, and high alcoholic sweetness and wine flavor coordination are synchronously achieved in a pure biological fermentation framework.

The invention discloses escherichia coli BLCY-018 and application thereof, and belongs to the technical field of biology. Escherichia coli BLCY-018 is preserved in China Center for Type Culture Collection on November 20, 2025, the address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province, and the strain preservation number is CCTCC NO: M 20252615; 4-alpha-glycosyltransferase can be produced, and the enzyme activity can be effectively improved by optimizing fermentation conditions; the prepared 4-alpha-glycosyltransferase can significantly improve the stability of the crystal structure of the low-DE-value dextrin, delay the aging of the low-DE-value dextrin and improve the stability, so that the solubility and viscosity of the dextrin are improved, the properties of the low-DE-value dextrin and the product state are more stable, and meanwhile, the 4-alpha-glycosyltransferase is more convenient to apply in a liquid state.

The invention discloses highly-reconstituted yolk yogurt freeze-dried powder and a preparation method thereof, and belongs to the technical field of food processing. The problems that existing yoghurt powder is low in strain activity and poor in reconstitution and reconstitution stability; in order to solve the technical problems that the application of egg yolk in food is seriously limited by the heat sensitivity of egg yolk protein, the fishy smell of eggs and the like, and the like, the preparation method comprises the following steps of: adding egg yolk liquid subjected to enzymolysis into a fermentation system by utilizing the emulsifying characteristic of the egg yolk, mixing the egg yolk liquid subjected to enzymolysis with cow milk for co-fermentation, and freeze-drying to process the yoghurt powder. During the period, the influence of the factors on the strain activity, texture, water-holding capacity, flavor and the like of the yolk yoghurt freeze-dried powder is researched by adjusting the types of enzymes, enzymolysis parameters, the addition amount of an enzymolysis yolk liquid, fermentation conditions and the like, and finally the yoghurt powder with high strain activity, high reconstitution, high reconstitution stability, high nutrition, good texture and good flavor is prepared.

The invention discloses recombinant poria cocos chitin endonuclease as well as a gene, a preparation method and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1 in a sequence table. According to the nucleotide sequence as shown in SEQ ID NO.1, high-level recombinant secretory expression of target protein in pichia pastoris host bacteria can be realized by taking pichia pastoris inducible expression plasmid pPICZ alpha A as a carrier. Under a shake flask fermentation condition, the average secretory expression quantity can reach 300.3 mg / L. The recombinant enzyme obtained by nickel ion affinity chromatography purification not only can effectively hydrolyze colloidal chitin to generate chitosan oligosaccharide (COS), but also can directly degrade chitin-containing raw materials such as shrimps, crabs and the like to generate COS. In addition, the enzyme also shows the activity of hydrolyzing polysaccharides such as cellulose, pectin, agar and the like, and has important potential values in the fields of functional chitosan oligosaccharide preparation, biological energy production, medicine development, feed, environmental protection, agricultural application and the like.

This invention discloses a method and product for preparing Pien Tze Huang herbal wine fermented on Cordyceps militaris culture medium, belonging to the field of health wine brewing technology. This method uses Cordyceps militaris culture medium (SCM) and Pien Tze Huang herbal wine (GFE) as raw materials, and prepares a health wine with hypoglycemic and antioxidant effects through a complex fermentation process including raw material pretreatment, enzymatic hydrolysis, yeast activation, anaerobic fermentation, and post-treatment. It aims to solve the problems of low raw material utilization, low active ingredient content, and insufficient antioxidant activity in existing technologies. Pretreatment employs ultrasonic extraction. Fermentation conditions are anaerobic fermentation at 28℃ for 18 days, with 3% sugar supplementation every 2 days, controlling the alcohol content at 6%-10% vol, and preferably using an SCM:GFE ratio of 2:1. The resulting product has an alcohol content of 8% vol, is rich in total phenols and total flavonoids, has an α-glucosidase inhibition rate of 82.6%, a DPPH free radical scavenging rate of 78.3%, and a FRAP value of 82.6 mM Fe. 2+ Equivalents / mL, with a harmonious flavor and significant health benefits, provide an effective way for the comprehensive development of Cordyceps militaris and Pien Tze Huang.

The application provides a huperzine A-producing strain of the genus of giant priestleya, namely, the Sys-Hup-009 strain, the strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, the preservation number is CCTCC NO:M20252404, and the 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The Sys-Hup-009 strain of the genus of giant priestleya provided in the application can metabolically produce huperzine A, can be subjected to large-scale fermentation culture in a short period, has low fermentation cost, is not limited by time domain, season and other conditions, and therefore can solve the problems of high cost, complicated steps and many intermediate pollutants in chemical synthesis of huperzine A through the microbial fermentation approach. Meanwhile, the application also provides optimized microbial fermentation conditions and a formula of a culture medium, and the yield of huperzine A is high.

The invention discloses a preparation method and application of high-activity recombinant collagen, and relates to the field of biological medicine, and the preparation method comprises the following steps: S1, providing engineering bacteria containing a recombinant collagen expression vector, and performing seed culture; s2, inoculating the seed solution into a fermentation culture medium for fermentation culture; s3, in the fermentation process, when the thallus density reaches a preset value, a signal factor without an animal source is added into a fermentation system, and the signal factor is bionic polypeptide; s4, after the signal factors are added, the fermentation conditions are adjusted, and target collagen expression is induced; according to the preparation method and the application of the high-activity recombinant collagen, an endogenous metabolic network of bacteria is actively regulated and controlled by accurately adding animal-source-free bionic polypeptide signal factors in the fermentation process of engineering bacteria, and correct folding and specific post-translational modification of target collagen are induced; therefore, the hydroxylation rate and the biological activity of the recombinant collagen are improved, and the structure and the function of the recombinant collagen are closer to those of natural collagen of a human body.

The invention relates to the field of tea leaf processing equipment, in particular to fermentation equipment for tea leaf processing, which comprises a fermentation box, a supporting assembly arranged in the fermentation box and a driving assembly used for driving the supporting assembly to rotate, and the supporting assembly is provided with a placing frame used for placing tea leaves needing to be fermented. The multiple supporting assemblies and the multiple placing frames are arranged, the multiple placing frames are arranged at intervals in the height direction of the fermentation box, and the multiple placing frames and the multiple supporting assemblies are in one-to-one correspondence; the supporting assembly comprises a supporting rod rotationally connected to the fermentation box, a supporting groove formed in the supporting rod and a supporting strip connected into the supporting groove in a sliding mode, the supporting strip is fixed to the side face of the containing frame, and the multiple containing frames are made to rotate from the horizontal state to the inclined state in the process that the driving assembly drives the supporting rod to rotate. The tea fermentation box has the effect that the tea fermentation condition in the fermentation box is convenient to observe.

The invention discloses a synthesis method of Peniterpheyl A. According to synthesis of a key structural unit furan ring, diphenyl ether is synthesized through a simple heating substitution reaction of a phenol derivative and a fluorobenzene derivative, then C-C direct oxidative coupling of double benzene rings in a molecule is achieved under a Pd / Ag catalytic system to synthesize the furan ring, and due to steric hindrance and guiding group effects, the furan ring can be synthesized into the furan ring. The product has high selectivity, does not need harsh reaction conditions, has simple synthesis steps, is suitable for large-scale production, does not need to widely screen specific strains and fermentation conditions compared with a method for obtaining a low-amount target compound from microbial fermentation separation, is simple and efficient, and can guarantee a large amount of compounds required by subsequent research and development.

This invention discloses a fermented wine made from Gastrodia elata, its preparation method, and its applications, belonging to the field of fermented wine preparation technology. This invention discovered that *Saccharomyces cerevisiae* SY exhibits faster fermentation speed, the lowest proportion of bitter amino acids, a higher proportion of umami amino acids, and a higher sensory score when preparing fermented wine from Gastrodia elata. It also retains the characteristic volatile substances of Gastrodia elata in moderate amounts, has minimal impact on the aroma of the wine, and produces a richer aroma profile, making it more suitable for producing fermented wine from Gastrodia elata. Furthermore, the fermentation process was optimized through single-factor experiments and response surface methodology. Finally, based on actual conditions, the optimal fermentation process was determined to be a fermentation temperature of 26.3℃, an initial sugar content of 21.5 °Bx, and a yeast addition of 0.14%. Under these fermentation conditions, the resulting fermented wine from Gastrodia elata has a bright yellow color, a strong fruity aroma, and a well-integrated flavor between the Gastrodia elata and the wine aroma, resulting in a distinct flavor profile. This invention provides technical support for developing fermented wines from Gastrodia elata with unique flavor and stable quality, promoting the application of Gastrodia elata in the field of deep processing.

The invention discloses a pinene synthase mutant and application thereof in pinene production, and belongs to the technical field of bioengineering. Isoleucine at the 409th site of pinene synthase is mutated into valine, phenylalanine at the 443rd site of pinene synthase is mutated into alanine, overexpression is carried out in serratia marcescens (HBQA7), and an engineering strain ST17 is constructed. Under the condition of shake flask fermentation, the pinene yield of the engineering strain ST17 reaches 0.80 g / L; the yield is obviously increased to 43.2 g / L through amplification culture in a 30 L fermentation tank. The invention provides an efficient, economic and environment-friendly novel method for industrial biosynthesis of pinene.

This invention belongs to the field of functional food fermentation technology, specifically relating to a method for fortifying peanut shells with luteolin and resveratrol through fungal fermentation, the resulting products, and their applications. The specific steps of this method are as follows: First, it was found that the relative contents of luteolin and resveratrol increased after co-fermentation of Ganoderma lucidum and peanut shells. Subsequently, by optimizing the fermentation and extraction conditions, the contents of resveratrol and luteolin in the fermented extract were increased. This method utilizes the bio-fermentation of edible and medicinal fungi, which utilizes components such as lignin in peanut shells to promote the growth of Ganoderma lucidum, and also releases luteolin and resveratrol, which are difficult to extract from peanut shells. The fermented extract contains both beneficial substances from Ganoderma lucidum and the two beneficial substances, luteolin and resveratrol, from peanut shells, with a 28.57% increase in resveratrol content and a 43.08% increase in luteolin content.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The invention relates to a method for efficiently synthesizing lysine by corynebacterium glutamicum based on multi-level metabolism reconstruction, and belongs to the technical field of metabolic engineering. The method is not limited to single gene or single path transformation, but carries out system integrated regulation and control from multiple levels of lysine main synthesis pathway, central carbon metabolism redistribution, by-product synthesis inhibition and lysine transport and tolerance mechanism, and constructs a ''flux-energy-tolerance'' synergistically matched integral metabolism network; the method is a fundamental technical framework for realizing 240g / L high yield. On the premise that genetic stability of engineering bacteria and controllability of the fermentation process are guaranteed, stable and efficient synthesis of lysine under the high-density fermentation condition is achieved, the final fermentation concentration of lysine stably reaches 240 g / L or above, meanwhile, high carbon source conversion efficiency and low by-product generation level are both considered, and the method is suitable for industrial production. Therefore, the technical bottleneck that the existing lysine biological manufacturing technology is limited to the platform stage of yield of about 200 g / L for a long time is broken through.