171 results about "Fermentation condition" patented technology

The invention discloses recombinant escherichia coli with high yield of N-acetylneuraminic acid and application of the recombinant escherichia coli, and relates to the technical field of biological genetic engineering. The invention relates to a recombinant escherichia coli, which is characterized in that the escherichia coli is taken as a host, and free expression of an N-acetylmannosamine epimerase gene yihS from Streptomyces xiamenensis or an N-acetylmannosamine epimerase gene ce3 from Bacteroides polymorpha and an exogenous N-acetylneuraminic acid lyase gene nano A is carried out; and carrying out recombinant expression on N-acetyl hexosamine 1-kinase nahK, a UDP-N-acetyl glucosamine pyrophosphorylase gene glmU and a UDP-N-acetyl glucosamine-2-epimerase gene neuC in the other synthetic route of the ManNAc. According to the recombinant escherichia coli with high yield of N-acetylneuraminic acid, the yield of N-acetylneuraminic acid can reach 23.08 g / L under a shake flask fermentation condition; a two-stage batch feeding strategy is adopted, the yield of N-acetylneuraminic acid in a 5L fermentation tank reaches 71.25 g / L, the molar conversion rate of GlcNAc reaches up to 57.60%, and the method has the potential of industrial application.

The invention discloses a scalp care solution with the effects of controlling oil, relieving, inhibiting bacteria and adjusting scalp micro-ecology and a preparation method and application thereof.The preparation method of the scalp care solution comprises the following steps that a fermentation substrate is inoculated with saccharomycetes, primary fermentation culture is conducted for 24-72 h, then secondary fermentation culture is conducted through Lactobacillus gasseri, and the scalp care solution is obtained; wherein the fermentation substrate comprises rice, barley seeds and water; wherein the saccharomycetes comprise Saccharomyces cerevisiae (Saccharomyces cerevisiae) and Saccharomyces fibulligera (Saccharomyces fibulligera), and the saccharomycetes comprise Saccharomyces cerevisiae (Saccharomyces fibulligera) and Saccharomyces fibulligera (Saccharomyces fibulligera). The rice and the barley seeds are fermented in a microbial fermentation mode, the bioavailability of the rice and the barley seeds is improved, the content of active ingredients in the fermentation liquor prepared through the fermentation technology is increased, the fermentation liquor has the ideal effects of controlling oil, resisting inflammation, inhibiting bacteria, adjusting scalp micro-ecology and the like, the preparation technology is simple, and the method is suitable for industrial production. The fermentation conditions are mild, no organic solvent is used, and safety is achieved.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

The invention relates to the technical field of microorganism co-culture fermentation, and discloses a method for producing L-carnitine soybean meal by applying a neural network to optimize fermentation conditions, and the method comprises the following steps: establishing a double-bacterium single-culture control group, collecting a double-bacterium dual-wavelength response data set, and constructing a two-dimensional geometric feature space to obtain a strain feature fingerprint slope; establishing a double-bacterium co-culture fermentation group, decomposing the real-time mixed optical density value to obtain double-bacterium estimated concentration, and constructing an enhanced input data set; then building a bimodal prediction model to output a predicted value of the L-carnitine content; and finally, setting an optimization objective function containing a yield deviation item, a phase regression item and a control smoothing item, and solving to obtain an optimal control input sequence. According to the invention, precise decoupling of the growth state of double bacteria and metabolic phase cooperative regulation and control are realized, information dimension limitation of traditional mixed optical density signals is broken through, and time sequence matching and yield stability of L-carnitine synthesis are guaranteed.

The invention discloses escherichia coli BLCY-018 and application thereof, and belongs to the technical field of biology. Escherichia coli BLCY-018 is preserved in China Center for Type Culture Collection on November 20, 2025, the address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province, and the strain preservation number is CCTCC NO: M 20252615; 4-alpha-glycosyltransferase can be produced, and the enzyme activity can be effectively improved by optimizing fermentation conditions; the prepared 4-alpha-glycosyltransferase can significantly improve the stability of the crystal structure of the low-DE-value dextrin, delay the aging of the low-DE-value dextrin and improve the stability, so that the solubility and viscosity of the dextrin are improved, the properties of the low-DE-value dextrin and the product state are more stable, and meanwhile, the 4-alpha-glycosyltransferase is more convenient to apply in a liquid state.

The invention discloses recombinant poria cocos chitin endonuclease as well as a gene, a preparation method and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1 in a sequence table. According to the nucleotide sequence as shown in SEQ ID NO.1, high-level recombinant secretory expression of target protein in pichia pastoris host bacteria can be realized by taking pichia pastoris inducible expression plasmid pPICZ alpha A as a carrier. Under a shake flask fermentation condition, the average secretory expression quantity can reach 300.3 mg / L. The recombinant enzyme obtained by nickel ion affinity chromatography purification not only can effectively hydrolyze colloidal chitin to generate chitosan oligosaccharide (COS), but also can directly degrade chitin-containing raw materials such as shrimps, crabs and the like to generate COS. In addition, the enzyme also shows the activity of hydrolyzing polysaccharides such as cellulose, pectin, agar and the like, and has important potential values in the fields of functional chitosan oligosaccharide preparation, biological energy production, medicine development, feed, environmental protection, agricultural application and the like.

This invention discloses a method and product for preparing Pien Tze Huang herbal wine fermented on Cordyceps militaris culture medium, belonging to the field of health wine brewing technology. This method uses Cordyceps militaris culture medium (SCM) and Pien Tze Huang herbal wine (GFE) as raw materials, and prepares a health wine with hypoglycemic and antioxidant effects through a complex fermentation process including raw material pretreatment, enzymatic hydrolysis, yeast activation, anaerobic fermentation, and post-treatment. It aims to solve the problems of low raw material utilization, low active ingredient content, and insufficient antioxidant activity in existing technologies. Pretreatment employs ultrasonic extraction. Fermentation conditions are anaerobic fermentation at 28℃ for 18 days, with 3% sugar supplementation every 2 days, controlling the alcohol content at 6%-10% vol, and preferably using an SCM:GFE ratio of 2:1. The resulting product has an alcohol content of 8% vol, is rich in total phenols and total flavonoids, has an α-glucosidase inhibition rate of 82.6%, a DPPH free radical scavenging rate of 78.3%, and a FRAP value of 82.6 mM Fe. 2+ Equivalents / mL, with a harmonious flavor and significant health benefits, provide an effective way for the comprehensive development of Cordyceps militaris and Pien Tze Huang.

The application provides a huperzine A-producing strain of the genus of giant priestleya, namely, the Sys-Hup-009 strain, the strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, the preservation number is CCTCC NO:M20252404, and the 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The Sys-Hup-009 strain of the genus of giant priestleya provided in the application can metabolically produce huperzine A, can be subjected to large-scale fermentation culture in a short period, has low fermentation cost, is not limited by time domain, season and other conditions, and therefore can solve the problems of high cost, complicated steps and many intermediate pollutants in chemical synthesis of huperzine A through the microbial fermentation approach. Meanwhile, the application also provides optimized microbial fermentation conditions and a formula of a culture medium, and the yield of huperzine A is high.

The invention relates to the field of tea leaf processing equipment, in particular to fermentation equipment for tea leaf processing, which comprises a fermentation box, a supporting assembly arranged in the fermentation box and a driving assembly used for driving the supporting assembly to rotate, and the supporting assembly is provided with a placing frame used for placing tea leaves needing to be fermented. The multiple supporting assemblies and the multiple placing frames are arranged, the multiple placing frames are arranged at intervals in the height direction of the fermentation box, and the multiple placing frames and the multiple supporting assemblies are in one-to-one correspondence; the supporting assembly comprises a supporting rod rotationally connected to the fermentation box, a supporting groove formed in the supporting rod and a supporting strip connected into the supporting groove in a sliding mode, the supporting strip is fixed to the side face of the containing frame, and the multiple containing frames are made to rotate from the horizontal state to the inclined state in the process that the driving assembly drives the supporting rod to rotate. The tea fermentation box has the effect that the tea fermentation condition in the fermentation box is convenient to observe.

This invention discloses a fermented wine made from Gastrodia elata, its preparation method, and its applications, belonging to the field of fermented wine preparation technology. This invention discovered that *Saccharomyces cerevisiae* SY exhibits faster fermentation speed, the lowest proportion of bitter amino acids, a higher proportion of umami amino acids, and a higher sensory score when preparing fermented wine from Gastrodia elata. It also retains the characteristic volatile substances of Gastrodia elata in moderate amounts, has minimal impact on the aroma of the wine, and produces a richer aroma profile, making it more suitable for producing fermented wine from Gastrodia elata. Furthermore, the fermentation process was optimized through single-factor experiments and response surface methodology. Finally, based on actual conditions, the optimal fermentation process was determined to be a fermentation temperature of 26.3℃, an initial sugar content of 21.5 °Bx, and a yeast addition of 0.14%. Under these fermentation conditions, the resulting fermented wine from Gastrodia elata has a bright yellow color, a strong fruity aroma, and a well-integrated flavor between the Gastrodia elata and the wine aroma, resulting in a distinct flavor profile. This invention provides technical support for developing fermented wines from Gastrodia elata with unique flavor and stable quality, promoting the application of Gastrodia elata in the field of deep processing.

This invention belongs to the field of functional food fermentation technology, specifically relating to a method for fortifying peanut shells with luteolin and resveratrol through fungal fermentation, the resulting products, and their applications. The specific steps of this method are as follows: First, it was found that the relative contents of luteolin and resveratrol increased after co-fermentation of Ganoderma lucidum and peanut shells. Subsequently, by optimizing the fermentation and extraction conditions, the contents of resveratrol and luteolin in the fermented extract were increased. This method utilizes the bio-fermentation of edible and medicinal fungi, which utilizes components such as lignin in peanut shells to promote the growth of Ganoderma lucidum, and also releases luteolin and resveratrol, which are difficult to extract from peanut shells. The fermented extract contains both beneficial substances from Ganoderma lucidum and the two beneficial substances, luteolin and resveratrol, from peanut shells, with a 28.57% increase in resveratrol content and a 43.08% increase in luteolin content.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The invention relates to a method for efficiently synthesizing lysine by corynebacterium glutamicum based on multi-level metabolism reconstruction, and belongs to the technical field of metabolic engineering. The method is not limited to single gene or single path transformation, but carries out system integrated regulation and control from multiple levels of lysine main synthesis pathway, central carbon metabolism redistribution, by-product synthesis inhibition and lysine transport and tolerance mechanism, and constructs a ''flux-energy-tolerance'' synergistically matched integral metabolism network; the method is a fundamental technical framework for realizing 240g / L high yield. On the premise that genetic stability of engineering bacteria and controllability of the fermentation process are guaranteed, stable and efficient synthesis of lysine under the high-density fermentation condition is achieved, the final fermentation concentration of lysine stably reaches 240 g / L or above, meanwhile, high carbon source conversion efficiency and low by-product generation level are both considered, and the method is suitable for industrial production. Therefore, the technical bottleneck that the existing lysine biological manufacturing technology is limited to the platform stage of yield of about 200 g / L for a long time is broken through.

The application discloses a kind of process methods for optimizing control production 5-aminovaleric acid, step one, amino acid fermentation seed is inoculated in culture medium, and aerobic fermentation culture is carried out;During fermentation culture process, fermentation temperature is controlled at 30-35 DEG C, pH value is maintained at 6.5-7.5, and by controlling aeration and stirring, dissolved oxygen level is controlled at 20%-30%;After fermentation, fermentation broth is separated, and 5-aminovaleric acid crude product is obtained;5-aminovaleric acid crude product is sequentially subjected to decolorization, crystallization and refining, and finally 5-aminovaleric acid finished product is obtained.The application uses optimized fermentation conditions, by accurately controlling fermentation temperature, pH value and dissolved oxygen level, to ensure the stability and efficiency of fermentation process, improve product quality and yield.

The invention discloses an oxidation-resistant Exiguobacterium sp. LS01 from a marine environment, and belongs to the technical field of microorganisms. The bacterial strain is classified and named as Exiguobacterium oxytolerans, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36750. The invention further discloses a preparation method of the Exiguobacterium oxytolerans strain. The invention further discloses application of the strain and a microbial inoculum of the strain, the strain can stably generate extracellular xanthan gum hydrolase with remarkable xanthan gum degradation activity under the fermentation condition, the viscosity of xanthan gum can be effectively reduced, and a remarkable degradation effect on the xanthan gum is achieved. The strain, the microbial inoculum and the fermentation product thereof can be applied to the fields of xanthan gum degradation and related food, and have wide industrial application prospects.

The invention discloses a Kluyveromyces marxianus strain and an application of the Kluyveromyces marxianus strain, and belongs to the field of synthetic biology and microorganism manufacturing. The strain has the characteristics of efficiently utilizing xylose and growing under a low-oxygen condition, and by taking the strain as a fermentation strain, the protein content of fermented straw powder is remarkably improved through the synergistic effect of the strain and cellulase under a straw powder high-solid fermentation condition. According to the yeast strain and the high-solid fermentation process thereof disclosed by the invention, the protein content of straw powder can be greatly increased, and an effective solution is provided for high-valued reutilization of straw agricultural wastes.

The invention provides a bacillus proteolyticus Sys-Gs-006 strain for producing ginsenoside Rh1, the strain is preserved in China Center for Type Culture Collection, the preservation date is December 9, 2025, and the preservation number is CCTCC NO: M20252815; the 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus proteolyticus Sys-Gs-006 strain provided by the invention can produce ginsenoside Rh1 through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domain and season, and has remarkable advantages. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of the ginsenoside Rh1 is relatively high.

The invention relates to the technical field of biological medicine, and discloses a preparation method and application of a novel spironolactone derivative based on intestinal fungus biotransformation, and the method comprises the following steps: S1, screening an intestinal fungus Syncephalasetum monosporium as a transformation strain; s2, carrying out in-vitro fermentation culture on the screened strains; s3, extracting metabolites in the fermentation liquor; s4, identifying and separating the novel spirolactone derivative by combining mass spectrometry and a chromatographic separation technology; and S5, verifying the aldosterone receptor antagonistic activity of the derivative through molecular docking simulation. A novel spirolactone derivative is identified and separated through strain screening, fermentation condition optimization, multi-stage purification and mass spectrometry and chromatographic separation technologies, the enzyme activity of the strain is activated through shake culture in a culture medium to promote metabolism, the product purity is improved through inactivation, extraction and chromatographic purification, and the problems that a product is single and low in purity in a traditional method are solved.

The invention relates to an N-acetylneuraminic acid fermentation method capable of reducing fermentation byproducts, and belongs to the technical field of fermentation. Escherichia coli is used as a chassis strain for fermentation synthesis of N-acetylneuraminic acid, sodium hexametaphosphate is added in the fermentation process to reduce generation of byproducts in the synthesis process of N-acetylneuraminic acid, and fermentation conditions are further optimized to improve the synthesis yield of N-acetylneuraminic acid. According to the fermentation method, the N-acetylneuraminic acid can be efficiently synthesized, the yield of the N-acetylneuraminic acid is increased, meanwhile, by-products difficult to separate are reduced, and a technical basis is provided for subsequent fermentation amplification and industrial production of the N-acetylneuraminic acid.

The invention relates to the field of gene engineering, and discloses an Aspergillus niger, a construction method and application thereof, and a method for producing citric acid. The Aspergillus niger does not express the RNA binding protein gene ssd1 in an original strain. According to the Aspergillus niger provided by the invention, multiple technical effects of remarkably reducing the diameter of a fungus ball, greatly reducing the sporulation quantity and remarkably improving the production efficiency of citric acid under a liquid submerged fermentation condition are realized, so that the problems of low mass transfer efficiency, long fermentation period and high spore pollution risk in the prior art are effectively solved; the invention provides an efficient, environment-friendly and genetically stable novel strain solution for industrial high-yield citric acid production.

This invention discloses a nicotinamide phosphoribosyltransferase mutant, a recombinant strain, and a method for constructing the same. The mutants are I457V, G344A, Q378A, T283G, or F182A, obtained by single-point mutation of the wild-type nicotinamide phosphoribosyltransferase shown in SEQ ID NO:1. This invention utilizes a method derived from... Schizotequatrovirus By rationally designing and site-directed mutagenesis of KVP40 nicotinamide phosphoribosyltransferase, combined with metabolic engineering to block the NMN degradation pathway and enhance substrate synthesis and product transport, the yield of β-NMN by the recombinant strain during shake-flask fermentation reached 4.82 g / L. Through optimization of fed-batch fermentation conditions, the highest yield of β-NMN during fed-batch fermentation in shake flasks reached 15.60 g / L.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing zeaxanthin as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, the yarrowia lipolytica is subjected to genetic engineering modification based on a genetic engineering technology, a zeaxanthin biosynthetic pathway is constructed by expressing beta-carotene hydroxylase in an original strain, and meanwhile, a zeaxanthin biosynthetic pathway is constructed through a multiple metabolic engineering modification strategy. The method comprises the steps of key enzyme PaCrtZ multi-copy integration, oxidation-reduction balance regulation and control, upstream mevalonic acid pathway enhancement through membrane-free organelles and the like, and the yield of zeaxanthin is further increased. Meanwhile, by optimizing the culture conditions and applying the optimal fermentation conditions to perform tank fermentation, the yarrowia lipolytica can continuously generate more zeaxanthin, so that the yarrowia lipolytica has excellent practical application value and application prospect.

This invention relates to the field of turning equipment technology, specifically a turning machine for organic fertilizer production. It includes a shell, which is V-shaped and open at both ends and the bottom. A traction device is located at the bottom of the shell, and a transmission system is located on one side of the shell. Inside the shell are a feeding device, a conveying device, a crushing device, a spraying device, a cleaning device, and a washing device. The feeding device, conveying device, crushing device, and cleaning device are connected by a transmission mechanism. This invention integrates the core functions of magnetic separation, bacterial agent addition, water replenishment, dispersing, self-cleaning, and turning into one integrated system. These functions work in synergy, achieving "one-time turning, multiple processes," eliminating the need for manual coordination between different operations. This optimizes fermentation conditions, simplifies the production process, and improves the production efficiency of bio-organic fertilizer.

This invention discloses a strain of *Propionibacterium freundii* and its application in propionic acid production. The strain was deposited at the China Center for Type Culture Collection on October 22, 2025, with accession number CCTCC M 20252289. This invention obtains a high-propionic acid-producing *Propionibacterium freundii* strain through screening from nature. Its 16S RNA was identified as *Propionibacterium freundii*. This strain is a safe edible strain approved by the Ministry of Health. Through optimization of its culture and fermentation conditions, the final propionic acid yield can reach over 50 g / L, the glucose conversion rate can reach 60%, and there is no lactic acid accumulation, meeting the requirements of industrial production. Furthermore, through fermentation optimization, the content of other acids can be effectively controlled, resulting in propionate products of different specifications that meet market demands.

The invention belongs to the technical field of microorganisms, and particularly discloses a strain of lactobacillus plantarum and application thereof, and the preservation number of the lactobacillus plantarum SN21 is CCTCC (China Center For Type Culture Collection) NO: M20252076. The lactobacillus plantarum SN21 provided by the invention is applied to fermentation of the peanut protein, the antigenicity of the peanut protein can be effectively reduced by optimizing fermentation conditions, and the oxidation resistance of a product is improved; meanwhile, the lactobacillus plantarum SN21 provided by the invention is used for fermenting peanut protein, and the solubility and the emulsifying property of the lactobacillus plantarum SN21 are improved. The strain has a good application prospect in development and utilization of fermented foods, and particularly has a relatively good application value in development of low-sensitivity peanut products and improvement of functional characteristics.

The present application relates to the technical field of biological fermentation detection, and discloses a kind of biological fermentation product kind identification method and system based on image recognition.The pixel offset of real-time image relative to background reference image is extracted to generate the first characteristic vector field;Response liquid level fluctuation obtains the time sequence image sequence of inner wall in preset boundary area, and the residual coverage rate decay rate constant is extracted to generate the first characteristic index;Based on real-time image gray information entropy, generate environment sampling operator.Three nonlinear weighted coupling generates species identification comprehensive index, realizes the dynamic weight inhibition of airspace scattering interference and the synchronous quantification of interface adhesion dynamics characteristics, guarantees the stability of species identification conclusion under complex fermentation conditions.

The utility model relates to the technical field of biological fermentation, in particular to a temperature and humidity system for a whole grain fermentation bed, which comprises a fermentation bed and a frame, the fermentation bed is mounted on the frame, the temperature and humidity system further comprises a humidifying mechanism and a heating and heat-preserving mechanism, the humidifying mechanism is mounted in the fermentation bed and used for humidifying fermentation materials or germinated seeds, and the heating and heat-preserving mechanism is used for heating the fermentation materials or germinated seeds. The heating and heat preservation mechanism is arranged on a shell of the fermentation bed; the humidifying mechanism comprises a top humidifying part, a middle humidifying part and a bottom humidifying part, the top humidifying part is arranged at the top in the fermentation bed and located above the material placing plate, the middle humidifying part is installed in the middle of the side wall of the fermentation bed, and the bottom humidifying part is arranged at the bottom in the fermentation bed and located below the material placing plate; and the material placing plates are arranged in multiple layers up and down. The humidifying mechanism is arranged, so that the fermentation bed is humidified in all directions, a more favorable fermentation condition is provided for material fermentation, and each humidifying part works independently, so that a stable humidity condition can be provided for material fermentation.

The invention relates to a lactic acid bacteria composition and an application thereof in a low-temperature liquid biological feed, in particular to a lactic acid bacteria complex microbial inoculant suitable for a low-temperature environment (15-25 DEG C) and a fermentation process of the lactic acid bacteria complex microbial inoculant. The combination comprises pediococcus pentosaceus WP101 (the preservation number is CCTCC NO: M 20241055) and lactobacillus plantarum PC01 (the preservation number is CCTCC NO: M2022106), and the viable count of the combination is 25 million cfu / g feed according to the proportion of 1: 1. By optimizing the synergistic effect of strains and fermentation conditions, efficient fermentation in a low-temperature environment is realized, the content of lactic acid in the feed is remarkably increased, and growth of harmful microorganisms is inhibited. Animal experiments show that the fermented feed can reduce the diarrhea rate of piglets by 45.5% (Plt; 0.05), improve the daily gain of fattening pigs in the later period and the feed conversion rate, and reduce the loss of dry matters at the same time. The problem of energy consumption caused by the fact that traditional medium-temperature fermentation depends on heating and heat preservation is solved, technical support is provided for large-scale low-temperature liquid biological feed production, and economic and environmental benefits are achieved.

The invention discloses a fermentation method for fungus-enzyme synergistic treatment of lucid ganoderma and application of the method in pet food. The fermentation method comprises the following steps: (1) enzymolysis pretreatment: mixing ganoderma lucidum sporocarp powder with water, adding cellulase and laccase, and performing enzymolysis to obtain an enzymolysis solution; (2) sterilizing and supplementing a carbon source: adding the carbon source into the enzymatic hydrolysate, and sterilizing; (3) lactobacillus fermentation: adding lactobacillus plantarum into the sterilized enzymatic hydrolysate, and fermenting under a fermentation condition to obtain fermentation liquor; and (4) solid-liquid separation: performing centrifugal separation on the fermentation liquor, and collecting supernate to obtain the ganoderma lucidum fermentation liquor. According to the method, the laccase and the cellulase are synergistically broken, so that the release efficiency of triterpenes and total phenols in the ganoderma lucidum with spores is remarkably improved. The fermentation liquor can improve lipid accumulation (the oil red O staining lipid content is reduced) and relieve oxidative stress injury on the cell level, and can be used for preparing functional food for improving metabolic health, especially for lipid abnormality and oxidative stress state related to metabolic dysfunction of pets.