1399 results about "Fermentation condition" patented technology

A method is provided to produce biodiesel from algae using a two-stage, autotrophic and heterotrophic cultivations of chlorella for biodiesel production. This method includes a sequence of procedures: cultivating photoautotrophic algae, concentrating cells and then transferring them to a fermentor for heterotrophic cultivation. During the photoautotrophic cultivation stage, the culture is exposed to a light source, such as sunlight with carbon dioxide obtained from a carbon dioxide source or from air. antibacterial agents may be added to prevent contamination from undesired microorganisms. Organic carbons are added during heterotrophic cultivation stage. Fermentation conditions are optimized for maximizing lipid synthesis. High biomass is achieved to about 108 g/L with lipid content reaching about 52% of dry cell weight. After cultivation, biodiesel is made through extraction and transesterification of algae lipids.

The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

The invention provides a composting method for biologically dehydrating livestock manure, which comprises the following steps: under the condition of adding biological fermentation strains, horizontally laying the livestock manure, adding fly maggots for fermenting, and then, adding the livestock manure and biological fermentation strains in batches, wherein the fermentation period is 3-7 days, and the fermentation condition is 22-42 DEG C. The invention also provides a composting method for biologically dehydrating the livestock manure, which comprises the following steps: under the condition of adding the biological fermentation strains, horizontally laying the livestock manure, and then, adding the fly maggots for fermenting, wherein the fermentation period is 7-12 days, and the fermentation condition is 10-22 DEG C. The invention reduces the initial water content of livestock manure composting by microbiological synergistic action, fully performs the action of composting fermentation strains, does not need to add auxiliary materials for regulating the water content of the livestock manure, reduces the treatment cost of the livestock manure, and improves the composting quality. Meanwhile, the culture of fly maggots provides a large amount of high-quality feed protein and raw materials for further processing to better recycle organic wastes, thereby realizing the purposes of protecting environment and increasing efficiency and yield agriculturally.

The invention discloses an extraction method for a tobacco extract and applications thereof. The invention is characterized in that the tobacco extract is produced by the following steps: step A, tobacco powder is sterilized by steam; step B, an external complex enzyme is accessed for zymohydrolysis; step C, a compound strain is accessed for fermentation; and step D, ethanol is used for extracting, filtering and condensing for obtaining the tobacco extract. In the invention, the complex enzyme is used for processing and the technique of microbial fermentation is combined; therefore, the production condition is moderate, the efficiency is high and the pollution is small; the strain is the commercialized strain commonly seen in the food industry; the purity is high and the price is low; and the tobacco extract is easy to be obtained and the fermentation condition is easy to be grasped and controlled. The tobacco extract obtained by the invention has excellent stability and repeatability; moreover, the tobacco extract has more remarkable fragrant increasing effect and specific style characteristics. The method is simple in production technique, low in production cost is easy to realize commercial production.

The microbe fermented feed is produced through preparing fermenting agent with several kinds of microbial strains, mixing supplementary material, straw and activated fermenting agent, fermentation inside one container of fermenting pond. The fermenting agent has wide application range of fermentation conditions and various straw and vine, green or dry, of different crops may be used as the main material for producing the fermented feed. The fermented feed has good feeding effect, and is delicious and easy to digest and absorb.

The invention relates to fermentation waste vegetables feed and a preparation method. The fermentation waste vegetables feed consists of mixed raw materials and microorganism living bacteria liquid, wherein the mixed raw materials consist of vegetable waste materials, straw and corn powder; and each ton of the mixed raw materials are formed according to the weight ratio (w/w): 70 to 80 percent of waste vegetables, 20 to 25 percent of straw, and 4 to 6 percent of corn powder. Each ton of the mixed raw materials are added with 30 to 40 milliliters of the microorganism living bacteria liquid, and the content of living bacteria for the microorganism living bacteria liquid is between 1.0x10<9>cfu/mL and 1.0x10<11>cfu/mL. The fermentation waste vegetables feed and the preparation method have the advantages of reasonable formulation, scientific production method, wide applicable areas, simple operation, easy master, low cost, reasonable formula design, convenient production, suitability for broad peasant households and livestock breeding plants, safe and no toxity, and great adaptability to fermentation conditions, and can be used as feed of cows, sheep, geese, and so on, make full use of side products in agriculture industry, realize reasonable use of the resources, and provide new techniques for clean and sustainable agricultural industry.

The invention discloses an Actinoplanessp. strain and its use in preparation of fidaxomicin. The Actinoplanessp. strain is entophytic Actinoplanessp. N12W0304, is preserved in the China general microbiological culture collection center (CGMCC) on December 26, 2012 and has a preservation number of CGMCC No.7043. A fidaxomicin production process comprises the following steps of preparing an Actinoplanessp. N12W0304 seed solution and fermentation broth, carrying out centrifugation of the fermentation broth to obtain mycelia, carrying out extraction, condensation, dissolution, filtration, resin adsorption and drying of the mycelia to obtain a fidaxomicin crystal crude product, dissolving the fidaxomicin crystal crude product, and carrying out liquid chromatographic separation and pressure-reduction drying of the solution to obtain a fidaxomicin refined product. Under the fermentation conditions, the Actinoplanessp. strain has a fidaxomicin fermentation unit which is more than 1400mg/L and is higher than the fidaxomicin fermentation yield reported at present. The fidaxomicin fermentation process provided by the invention is simple, is suitable for industrial production and realizes a total product yield of 60%.

The invention provides a process for producing lobster sauce. The process comprises the following steps: a, cooking the soaked soybean into cooked material; b, cooling down the cooked material to the temperature of between 40 and 50 DEG C, and inoculating and culturing aspergillus oryzae strain until the surface of the lobster sauce grain is suffused with hypha to obtain bean fermented preparation; c, evenly mixing the bean fermented preparation with auxiliary materials commonly used for preparing the lobster sauce and stood until no water is obviously seeped out to obtain the bean wine; and d, placing the bean wine in a sealed container and fermenting the bean wine for not more than 12 days to obtain the finished product. Compared with the prior art, the process has the advantages of obviously short fermentation time, simple equipment, easy control fermentation conditions because of small fermentation container, and low labor intensity of workers. For the lobster sauce produced by the process, the water soluble protein content is close to that of the lobster sauce produced by the prior process, but the lobster sauce produced by the process has high finished product rate and fragrance of fermented soybean, and is salty, fresh and sweet and is not acrid; moreover the lobster sauce also has hard core and formed and separate seed, is black brown and good in brightness, and meets relevant national standards.

The invention relates to a method for utilizing nano ferroferric oxide for improving the activity of anaerobic digestion methanogens and methanogenesis efficiency. The method aims at solving the problems that in the prior art, the addition concentration of Fe2+/Fe3+ is hard to control, negative ion inhibition is caused, bioavailability is low, the chemical property is unstable, and cost is high. The method comprises the steps that short chain fatty acid salt is adopted as an electron donor, amine salt is adopted as a nitrogen source, phosphate is adopted as a phosphorus source, system basicity is maintained, and under the culture condition of continuous flow anaerobic fermentation, anaerobic mixed granule sludge is separated, and mixed seed sludge is obtained; the short chain fatty acid salt is adopted as the electron donor, the amine salt is adopted as the nitrogen source, the phosphate is adopted as the phosphorus source, the system basicity is maintained, the nano ferroferric oxide is adopted as an iron ion donor, the mixed seed sludge is adopted as inoculation sludge, and culturing is carried out under the static anaerobic fermentation condition. The method is used for improving the activity of the anaerobic digestion methanogens and the methanogenesis efficiency.

2,3-BDO is important industrial chemicals and a medicinal precursor, for example, the 2,3-BDO can form 1,3-butadiene through dehydration and can be used for synthesizing rubber, polyester and polyurethane; the esterified 2,3-BDO is a precursor of synthetic polyimine, and can be applied to medicaments, cosmetics, washing liquor liquid and the like; and in addition, the 2,3-BDO can also be used as a flavor substance to be added into distilled spirit and cream. The 2,3-BDO can be prepared by a chemical synthesis method and a microorganism production method, and due to a special structure of 2,3-butanediol, in the chemical method for producing the 2,3-butanediol, the 2,3-butanediol is mainly prepared by hydrolyzing four-carbon hydrocarbon generated during the splitting decomposition of petroleum at the high temperature and under the high pressure and has the disadvantages of high cost, complicated processes and difficult operation, so the large-scale industrial production is difficult to realize all the time. With the increasing rise in oil price, people pay close attention to the production of the 2, 3-butanediol by using the microbial fermentation method and the developmental research of derivatives thereof gradually. The fermentation method for producing the 2,3-butanediol has the following advantages that: firstly, the fermentation method has the superiority in cost, and the chemical method has high production cost; secondly, the fermentation method has an environmental-friendly characteristic, and the 2,3-butanediol is used as an organic synthesis intermediate to occupy markets of a number of compounds and other petroleum chemicals, has wide prospect, obvious social and environmental benefits, and is particularly favorable for the extensive application of the products; and thirdly, in the fermentation method for producing the 2,3-butanediol, renewable resources are used as raw materials, so the fermentation method does not depend on the petroleum chemicals.

The invention discloses a Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and a fermentation method thereof. The Schizochytrium sp.TIO1101 strain provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4603. After the Schizochytrium sp.TIO1101 is subjected to high-density heterotrophic fermentation for 72h, the biomass liveweight, the lipid content and the DHA are respectively 120g/L, 54.3% and 51.4% of the content of total lipid, therefore the Schizochytrium sp.TIO1101 has development and application values extremely. The Schizochytrium sp.TIO1101 disclosed by the invention has the advantages that: the Schizochytrium sp.TIO1101is obtained through separation; the high-density heterotrophic fermentation of the Schizochytrium sp.TIO1101 is realized; and the lipid extracted from the Schizochytrium sp.TIO1101 cells obtained under the fermentation condition has high DHA content and purer components.

The invention discloses a wine-making preparation method adopting litchi fermentation. The method comprises the following steps: adopting pollution-free excellent quality litchi pulp to make fruit juice concentrate by means of a low-temperature ice-crystal method or a membrane concentration method, and blending the fruit juice concentrate with high dry wine; adding highly concentrated fruit juice frozen by the low-temperature ice-crystal method or the membrane concentration method into the mixture to keep the typical aroma of litchi fruit wine; adopting yeast strain, lactic acid strain and acetic acid strain to carry out multi-strain coordinated fermentation and adjusting the fermentation conditions; accelerating fermentation and conversion by fruit sugar and glucose cooled after high-temperature acid conversion of sucrose to improve the quality of fermented wine liquid; making use of ultra low temperature cryoprecipitation and triple filtration sterilization to solve the problem of deposition of products during storage and transportation; and adopting non-thermal sterilization filtration and concentration membrane technology to avoid the influence on fruit wine quality caused by thermal sterilization. The wine-making preparation method can obtain a natural litchi fermented wine product with palateful quality.

The invention discloses a remote multi-dump-pit data acquisition and recording system and belongs to the technical field of garbage processing and computer programs. Humiture of dump pits, level data and states of such devices as a garbage crane and the like are obtained from a PLC periodically by use of a polling strategy and are stored, a preset dump pit subarea is divided into a discharging area, a raw material area, a fermentation area, a clinker area and a percolate collection area, the garbage crane, as an execution mechanism, performs garbage carrying and turning work between the areas, a background computer, according to a built-in preset condition, analyzes obtained detection data, a slaking subarea model of garbage fermentation is formed, and correlation work of the garbage crane is guided. The method provided by the invention can effectively solve the problem of monitoring in real time the garbage fermentation condition in a dump pit, prevents garbage which is not sufficiently fermented and slaked from being sent to an incinerator for combustion, and thus the operation cost of a garbage burning factory is saved.

The invention discloses a method for processing starch wastewater and the application thereof. The method comprises the following steps: (1) mould is inoculated in starch wastewater for fermentation; and (2) saccharomycete is inoculated in the product obtained by fermentation in step (1) and the fermentation is continued to obtain the product. The mould comprises one or several out of aspergillus niger, Taiwan rhizopus and aspergillus oryzae. The fermentation conditions are as follows: after mould is inoculated in starch wastewater, the fermentation is performed for 16 hours at the temperature of 29.5 DEG C; the fermentation is continued after saccharomycete is inoculated and pH of fermentation broth is 5.0. The method utilizes the mutualism effect among microorganism and adopts the mixed culture fermentation method to process starch wastewater. The method can not only remove the main pollutants in starch wastewater, but also acquire single cell protein with high feed value, thereby realizing recycling processing of starch wastewater.

The invention relates to a high-density culture method of bifidobacterium lactis V9 and a preparation method of a freeze-dried powder of the bifidobacterium lactis V9, and belongs to the field of probiotics fermentation. The bifidobacterium lactis V9 (Bifidobacterium animalis subsp.lactis V9) is acid-resistant and cholate-resistant probiotics which are separated from the intestinal tract of a healthy Mongolian child, and the collection number of the bifidobacterium lactis V9 is CGMCC No.5470. High-density fermentation of the bacteria of the bifidobacterium lactis V9 is realized under the control of an optimized culture medium and optimized fermentation conditions; the viable count in a fermentation liquor can be more than 2.0*10<10>cfu/ml, and the viable count in the freeze-dried powder of the bifidobacterium lactis prepared by using the fermentation liquor can be more than 6.0*10<11>cfu/g, and therefore, the freeze-dried powder of the bifidobacterium lactis can be applied to a probiotic leavening agent or a probiotic preparation.

The invention discloses a rhamnolipid high-yielding bacterial strain, and applications thereof. The rhamnolipid high-yielding bacterial strain is named as pseudomonas aeruginosa KT1115, is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC M 2016686. The rhamnolipid high-yielding bacterial strain is stable in hereditary features and high in rhamnolipid yield, and is obtained via ARTP induced mutation, blue gel plate screening, and secondary screening. According to fermentation broth of pseudomonas aeruginosa KT1115, liquid surface tension is reduced to 24.8mN/m<2>, and emulsification value is as high as 80%. Optimization of fermentation conditions including carbon source and nitrogen source single factors, cheap crude glycerine and swill-cooked dirty oil are taken as a composite carbon source, so that production cost is reduced greatly. The rhamnolipid yield of pseudomonas aeruginosa KT1115 is 50.7g/L, is increased by 18.1 times compared with that of a starting strain, conversion rate is 0.75g/g, and is relatively high. Application prospect of pseudomonas aeruginosa KT1115 in further industrialized application is promising.

The invention aims to disclose a method for producing vitamin K2 by utilizing bacillus natto. Protoplast fusion is conducted on bacillus natto BS-53 highly yielding the vitamin K2 and bacillus natto CICC10262 having a high growth speed but a low vitamin K content, and fusants with parent advantages are screened out; obtained high-yield strains having a high growth speed are utilized to produce the vitamin K2 under an optimized fermentation condition; and isopropanol and n-hexane are utilized to extract and separate the vitamin K2. The growth speed of the strains and the yield of the vitamin K2 is increased and an effective method is provided for scale production of the vitamin K2, thereby realizing the aim of the invention.

The invention provides a method for preparing vegetable protein small peptide by microoprganism fermentation. According to the invention, soybean meal, peanut cake, cottonseed cake, rapeseed cake, zein powder, wheat glutelin protein powder, soybean protein isolate, soybean powder and the like are taken as raw materials, one raw material is used or more than two raw materials are mixed, macro-molecule protein in the plant is decomposed by the microoprganism fermentation to form micromolecule bioactive peptides, then spray drying is carried out to prepare the powdery vegetable protein small peptide product. The highly finished product can be used for food, cosmetics and health products. The crude product can be used as a feed functional reinforcing additive, a microorganism culture medium of the fermentation industry, a vegetation promoter and the like. The invention has the advantages of mature technology, reliable and stable technological parameters and mild fermentation condition, the final product has stable quality, and has the obvious biological function characteristic.

The invention discloses a horizontal-type solid fermentation tank and fermentation method for Pu-erh tea. The fermentation tank comprises a tank body supported by a tank body support, and a clamp jacket is installed outside the tank body; the top of the tank body is provided with feed inlets, exhaust ports, diversion plates connected with heating wires, a water inlet and a water replenishing and inoculation opening; the bottom of the tank body is provided with a discharge outlet, a water vapor inlet, a sterile air inlet, a carbon dioxide inlet, a drying ventilation opening and a water outlet, and a stirring system is further arranged in the tank body. According to the fermentation method, a traditional Pu-erh tea pile-fermentation technology is changed, production standardization and specification rationalization of the Pu-erh tea are achieved on the condition that fermentation conditions such as microorganism species, tidal prism, temperature, fermentation time, inoculated-pathogen quantities and oxygen and carbon dioxide content of the Pu-erh tea are comprehensively considered, the fermentation process of the Pu-erh tea is artificially controllable, and the quality of the Pu-erh tea is more stable, safer and more controllable.

The invention relates to saccharification processing technology of jerusalem artichoke by utilizing high-quality fructose biomass as a raw material and strain selection and technology optimization for producing mannitol by taking jerusalem artichoke as carbon source through fermentation. The method comprises the following steps: 1) crushing jerusalem artichoke tuber into coarse particles, filtering after water leaching and enzymolysis for 6 hours, supernating at 42 DEG C, rotating, evaporating and concentrating to obtain saccharification jerusalem artichoke juice with high concentration of fructose; 2) establishing high performance liquid chromatography analysis and detection conditions which can synchronously analyze the content of fermentation liquor substrate (glucose and fructose) and products (mannitol); and 3) inspecting the capacity for producing lactic acid and mannitol through fermentation by seven lactic acid bacteria by utilizing saccharification jerusalem artichoke juice with different concentration of total sugar, thus determining lactic acid bacteria with high transformation rate and production intensity of fructose, and optimizing production fermentation conditions and the highest concentration of tolerant substrate. Through feed-batch fermentation, production efficiency can be improved and mannitol can be continuously produced in large scale. The method not only generates no byproduct of sorbitol, but also has low production cost, wide raw material sources, simple technology, and mature technical route and can be implemented in industrialization.

The invention provides Lysobacter capable of efficiently degrading aflatoxin B1 and ochratoxin A and application of the Lysobacter and particularly provides double-function Lysobacter sp. CW239 and application thereof to the degradation of low-pollution concentration aflatoxin B1 and ochratoxin A. Compared with existing aflatoxin degrading bacteria, the Lysobacter sp. CW239 has the advantages that the Lysobacter sp. CW239 can achieve excellent degrading effect under a low-concentration toxin pollution condition; under a liquid fermentation condition, in fermentation broth, which contains the aflatoxin B1 and ochratoxin A, with the final concentration of 20 microgram/L, the 12-hour ochratoxin A degradation rate of the Lysobacter sp. CW239 is 53.1%, and the 48-hour degradation rate reaches 99.8%; the 12-hour aflatoxin B1 degradation rate of the Lysobacter sp. CW239 is 42.5%, and the 48-hour degradation rate reaches 83.4%; when the Lysobacter sp. CW239 is used for processing feed (with the final concentration of 20 microgram/kg) polluted by toxins, the 48-hour ochratoxin A degradation rate is 68.7%, and the 48-hour aflatoxin B1 degradation rate is 52.1%; the Lysobacter sp. CW239 has substantial application value and significance when being applied to food and feed bio-detoxification.

An improved process for obtaining value added by-products from an ethanol production whole stillage is disclosed wherein the whole stillage is mixed with additives to form a nutrition enriched product stream that is subsequently dried to produce a nutrition enriched product having a water content of less than 12% by weight. In a preferred embodiment the whole stillage is obtained from a ethanol production process that has utilized a corn flour as the starting feedstock. The corn flour is then hydrolyzed along with added vitamins and/or nutrients and/or enzymes. The hydrolyzed product is then preferably mixed with a soy hull flour, also preferably having a median particle size of less than about 100 microns, and subjected to conventional fermentation conditions to produce an ethanol containing product. The ethanol-containing product is then distilled to produce an ethanol enriched steam and the separate whole stillage to be used as the feedstock.