550 results about "Fermentation condition" patented technology

The invention relates to the technical field of monitoring control, and provides an intelligent monitoring method for a dandelion and golden camellia fermentation process based on the Internet of Things, which comprises the following steps: deploying and self-calibrating a sensor array, extracting and analyzing various data features, and judging a fermentation stage and generating a regulation and control instruction according to the data features. According to the method, a precise fermentation monitoring and control method logic is formed, through omnibearing data acquisition and deep analysis, the change of each parameter in the fermentation process can be precisely grasped, the hypha growth rate, the flora metabolism intensity, the generation of a target product and other key indexes are monitored in real time, the fermentation conditions are adjusted in time, and the fermentation efficiency is improved. And the fermentation process is stabilized in an optimal state, so that the accuracy of fermentation production is greatly improved, the fluctuation of product quality is reduced, and the consistency and stability of the quality of each batch of dandelion and golden camellia are ensured.

The invention discloses an intelligent regulation and control method for a tea fermentation process and a regulation and control system based on microbial activity monitoring, and relates to the technical field of tea processing. According to the method, microbial activity and environmental parameters are monitored in real time, and a microbial metabolism model and a neural network algorithm are combined, so that dynamic optimization and accurate regulation and control of the tea leaf fermentation process are realized, firstly, microbial activity data and environmental parameters in a fermentation environment are collected by utilizing a sensor array; the method comprises the following steps: firstly, calculating a metabolite generation rate and a concentration change trend through a microbial metabolism model, accurately identifying abnormal fluctuation in a fermentation process, secondly, adjusting fermentation conditions in real time based on the metabolite concentration change trend and a dynamic regulation and control algorithm to ensure the stability of the fermentation process, and finally, carrying out real-time fermentation. Tea quality parameters after fermentation condition adjustment are predicted through a neural network algorithm, a regulation and control strategy is optimized, and stable improvement of tea quality is achieved.

The invention relates to the technical field of production parameter control, and particularly discloses an organic fertilizer production parameter optimization control method for a compound microbial agent, which comprises the following steps of: calculating a temperature fluctuation index and a pH stability index according to temperature data and a pH value in a fermentation stage so as to quantitatively evaluate the stability of a fermentation environment; evaluating the external interference degree by using the normalized comprehensive influence factor, intelligently analyzing the microbial ecological disorder condition through clustering analysis and a decision tree algorithm in combination with microbial concentration data, and calculating a microbial ecological disorder fluctuation coefficient; based on an evaluation result, dynamically calculating an adjustment factor, accurately adjusting the temperature and the pH value in the fermentation condition, and ensuring that the microbial flora is in an optimal active state; finally, whether the fermentation parameters reach the standard or not is determined by re-evaluating the adjusted flora state, so that the quality and the production efficiency of the organic fertilizer are ensured.

The invention relates to the technical field of bioengineering fermentation, and discloses a bioengineering fermentation method, through multiple groups of sensors arranged in a fermentation reactor, key data parameters in the fermentation reactor can be detected and monitored in real time, an operator can be helped to quickly and accurately know the fermentation state of a material in the fermentation reactor, and the fermentation efficiency of the material in the fermentation reactor is improved. Real-time and reliable working parameter adjustment and control are achieved, a multi-parameter dynamic optimization type biological engineering fermentation method is adopted, key parameters in fermentation engineering are accurately analyzed and predicted through dynamic parameter collection in cooperation with a mathematical model and an algorithm, prediction is accurate and reliable, reliable data support can be provided for dynamic regulation and control, and the method is suitable for large-scale popularization and application. The fermentation conditions are dynamically adjusted, so that the growth and metabolism of microorganisms are optimized, the yield and quality of products are improved, the fermentation period is shortened, the production cost is reduced, reliable experience and data support can be provided for subsequent fermentation work in cooperation with data recording, and the fermentation efficiency and the product quality are further optimized.

The invention discloses bacillus capable of tolerating various organic solvents and application of the bacillus. The invention provides a bacillus sp. YL23 strain, and the preservation number of the bacillus sp. YL23 strain is CGMCC (China General Microbiological Culture Collection Center) NO.29579. Also provided is the use thereof in the preparation of recombinant microorganisms for industrial fermentation. YL23 has potential application value in the technical field of industrial biology, including that substitute raw materials such as xylose can be utilized to ferment and produce target chemicals under acidic and high-temperature conditions. The characteristic can realize high-temperature production in the field of fermentation, and the processes of sterilization and the like are reduced. Meanwhile, the acid fermentation condition and the good organic solvent tolerance characteristic are beneficial to accumulation of target products such as organic acid.

The invention relates to the technical field of automatic control, and discloses an automatic control method and system for production of a blood-sugar-reducing egg fermented feed. The method comprises the following steps: intelligently identifying raw materials, accurately proportioning, acquiring digital fingerprints by using multiple groups of sensors, and performing double-speed blanking to realize proportioning; feeding the materials into a parameterized mixing system, and performing multi-stage variable-speed stirring to generate a parameter report; self-adaptive fermentation, multi-point sensing monitoring and self-adaptive PID adjustment are carried out according to report conditions to obtain a fermentation material; and performing post-processing to obtain a feed product and a digital file. The technical problems of accurate identification and intelligent proportioning of raw materials in the production process of the blood-sugar-reducing egg fermented feed, uniformity control and accurate regulation and control of fermentation conditions in the mixing process of the functional feed and full-flow digital management and quality tracing of the blood-sugar-reducing functional feed are solved.

The invention provides a culture method for increasing the content of a bioactive substance Emodic acid in talaromyces purpurascens, and relates to the technical field of medicinal fungus culture. The culture method of the homopurple talaromyces spp. Comprises the following steps: carrying out liquid culture on hyphae of the homopurple talaromyces spp., and then inoculating the hyphae into a liquid culture medium containing black bean powder for culture. The defects in the prior art are overcome, and the pharmacological activity of the talaromyces purpurascens is enhanced by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, so that the content of Emodic acid in the talaromyces purpurascens is increased.

The invention relates to the technical field of sewage treatment, in particular to a method for improving the denitrification nitrogen removal performance of a corn or rice straw fermentation carbon source by using industrial glucose. According to the method, the straws are soaked and pretreated by adopting calcium peroxide, and meanwhile, in the anaerobic fermentation process, the anaerobic condition is kept through nitrogen, and zero-valent iron is added to obtain the straw fermentation liquor. Fermentation liquor and industrial glucose are mixed to serve as a composite carbon source, the activity of microorganisms in a sludge system is stimulated through the usability of the glucose, and the utilization rate of the microorganisms to the rice and corn fermentation liquor carbon source is increased; anaerobic fermentation conditions of rice and corn straws are optimized, the carbon source release rate is increased, the complexity of carbon source composition of fermentation liquor is utilized, the diversity of microorganisms is improved, excessive propagation of the microorganisms is avoided, and the biological denitrification capacity is improved. The method disclosed by the invention is beneficial to resource utilization of agricultural wastes, reduces the biological denitrification cost of sewage with a low carbon-nitrogen ratio, and promotes green and low-carbon development of sewage treatment.

The invention relates to the technical field of sewage treatment, in particular to a multistage fermentation acid production preposed phosphorus recovery device which comprises an aerated grit chamber, a fermentation primary sedimentation tank, a preposed sequencing batch type phosphorus removal system, a treatment and discharge system and a recovery system, the aerated grit chamber is connected with an external sewage pipeline, the fermentation primary sedimentation tank is arranged on one side of the aerated grit chamber, and the preposed sequencing batch type phosphorus removal system is arranged on the other side of the aerated grit chamber. The front sequencing batch dephosphorization system is communicated with the fermentation primary sedimentation tank, and the treatment and discharge system is communicated with one side of the front sequencing batch dephosphorization system. Compared with the prior art, by arranging the fermentation primary sedimentation tank and reasonably controlling the hydraulic retention time and the sludge concentration, not only are fermentation conditions in the tank optimized, but also unnecessary stirring energy consumption and excessive sludge treatment requirements are reduced, the operation cost is reduced, and meanwhile, the sludge load of a subsequent system is effectively reduced.

The invention belongs to the technical field of traditional Chinese medicine patches, mainly relates to a traditional Chinese medicine patch and a preparation method thereof, and in particular relates to a patch for treating cough and asthma and a preparation process thereof. Natural plants including the semen brassicae, the pink reineckea herb and the modified herba ephedrae are selected, the performance is improved through synergistic fermentation of the lactobacillus acidophilus CICC-22162 and the bifidobacterium breve CICC-6184, the effect of treating cough and asthma of the traditional Chinese medicine is improved, meanwhile, under the fermentation condition, the effect of treating diarrhea is achieved under the synergistic effect of leavening of the semen brassicae and the pink reineckea herb, and the traditional Chinese medicine composition has a good application prospect. In addition, the perillaseed extracting solution and the asarum extracting solution which are used in the patch have extremely high oxidation resistance under the synergistic effect, so that the stability of the patch is effectively improved, and the shelf life of the patch is prolonged.

The invention relates to the technical field of Chinese herbal medicine fermentation, and discloses a mixed strain fermentation method for efficient conversion of non-medicinal parts of traditional Chinese medicine, which comprises the following steps: constructing an intelligent fermentation system comprising a plurality of independent fermentation units, respectively culturing different strains such as aspergillus niger, bacillus subtilis, saccharomyces cerevisiae and the like in each fermentation unit, the online sensor is integrated in the system and used for monitoring key fermentation parameters in real time, and a fermentation model and an optimization algorithm are arranged in the system and can automatically adjust fermentation conditions. An intelligent fermentation system comprising a plurality of independent fermentation units is constructed, precise classification pretreatment and mixing according to a specific ratio are carried out on rhizoma polygonati stems and leaves and gentian stems and leaves, and an intelligent control system is utilized to dynamically regulate and control the fermentation process, so that the synergistic effect of different strains such as aspergillus niger, bacillus subtilis and saccharomyces cerevisiae is fully exerted, and the fermentation efficiency is improved. The dissolution rate and the conversion rate of active ingredients in the non-medicinal part of the traditional Chinese medicine are improved.

The invention discloses an Escherichia coli mutant strain which is classified and named as Escherichia coli, the strain number is CR-TK01, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35554. The invention further discloses a preparation method of the Escherichia coli mutant strain. The Escherichia coli CR-TK01 with high yield of cytidine provided by the invention has the characteristics of high yield and stable high-yield character, glucose is used as a carbon source, the Escherichia coli CR-TK01 is used for fermentation production of cytidine under a certain fermentation condition, and the yield of cytidine is as high as 97.23 g / L; the strain is not degraded after passage for more than 20 generations, the performance of high-yield cytidine is maintained in the passage fermentation process, and the yield of cytidine in passage fermentation is improved. The method for producing cytidine through fermentation is simple in process, high in yield and suitable for industrialization.

The invention provides a culture method for increasing the content of antroquinonol in antrodia camphorata mycelia, and relates to the technical field of medicinal fungus culture. The culture method comprises the steps that antrodia camphorata mycelia are inoculated into a liquid culture medium added with meliaceae cedrela sinensis tender leaf homogenate to be cultured, the liquid culture medium is a PDA culture medium, and the adding amount of the meliaceae cedrela sinensis tender leaf homogenate is 0.5-2 g / 100 mL. According to the method, the defects in the prior art are overcome, the content of antroquinonol in the antrodia camphorata mycelia is increased by optimizing the formula of the fermentation culture medium and improving the fermentation conditions, and the pharmacological activity of the antrodia camphorata mycelia is enhanced.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

The invention relates to the technical field of bio-organic fertilizer fermentation monitoring, in particular to a bacillus bio-organic fertilizer fermentation condition monitoring and optimizing method. The method comprises the steps of obtaining a data change degree characterization value and a related difference characterization value of a first detection point, obtaining a target bacillus acid production characterization value of the first detection point according to the data change degree characterization value and the related difference characterization value of the first detection point, and obtaining a target bacillus acid production characterization value of the second detection point according to the target bacillus acid production characterization value. Screening the first detection point again, obtaining a predicted PH value at a future monitoring moment according to a screening result, and monitoring the fermentation process of the bacillus bio-organic fertilizer according to the predicted PH value at the future monitoring moment. And the timeliness or timeliness of monitoring the pH value of the fermentation liquid in the fermentation tank can be improved by predicting the pH value.

The invention provides a method for promoting generation of tea acid from Nemania sp YAFEF112, and relates to the technical field of microbial culture. According to the method, mycelia of Nemania sp YAFEF112 are inoculated into a culture medium containing pruned walnut branches for culture, the culture medium is an MM culture medium, and the specific formula of the culture medium comprises 1.52 g / L of monopotassium phosphate, 0.52 g / L of magnesium sulfate, 6 g / L of sodium nitrate and 0.52 g / L of potassium chloride. According to the method, the defects in the prior art are overcome, and good conditions are provided for the production of the tea pickled acid by optimizing the formula of the fermentation culture medium, adding pruned walnut branches into the culture medium, improving the fermentation conditions and increasing the content of the tea pickled acid in the Nemania sp YAFEF112 hyphae.

The invention discloses a preparation method and application of a three-thorn fruit fermentation broth capable of regulating cell rhythm, and the preparation method comprises the following steps: (1) adding nitraria fruit, yellow thorn fruit and prinsepia utilis royle into a fermentation enzymatic hydrolysate, and carrying out enzymolysis to prepare a fermentation substrate; and (2) inoculating schizophyllum commune into the fermentation substrate for fermentation, and sterilizing to obtain the schizophyllum commune fermentation substrate, wherein a preparation method of the fermented enzymatic hydrolysate comprises the following steps: adding water into a substrate, sterilizing to obtain a culture medium, inoculating, performing aerobic fermentation, and centrifuging, thereby obtaining the fermented enzymatic hydrolysate. The fermentation enzymatic hydrolysate comprises agaricus blazei fermentation enzymatic hydrolysate or saccharomyces cerevisiae fermentation enzymatic hydrolysate. According to the method, saccharomyces cerevisiae or agaricus blazei fermentation enzymatic hydrolysate is firstly used for enzymolysis extraction, wall breaking is conducted on plants preliminarily, active ingredients in the plants are released, then schizophyllum commune is further fermented, further release and conversion are achieved, ideal effects of oxidation resistance, moisturizing, soothing, tightening, whitening and the like are achieved by adjusting cell rhythm, fermentation conditions are mild, and the method is suitable for industrial production. Good application prospects are realized.

The invention discloses a construction method of a heparin 2-O-sulfotransferase high-activity mutant, and belongs to the technical field of biology. According to the heparin 2-O-sulfotransferase and the application of the heparin 2-O-sulfotransferase, a dissolution promoting label is fused at the N end of the heparin 2-O-sulfotransferase, the coding sequence of the dissolution promoting label is optimized, and active expression of the heparin 2-O-sulfotransferase is achieved; according to the invention, the heparin 2-O-sulfotransferase is mutated, so that a mutant with high stability and catalytic activity is obtained; furthermore, fermentation conditions of the heparin 2-O-sulfotransferase are optimized, so that the expression quantity and the enzyme activity are improved, and the application potential of the heparin 2-O-sulfotransferase in industrial production is enhanced.

The invention discloses a production process of grain amaranth fermented feed, and belongs to the technical field of livestock breeding. The method comprises the following steps of raw material preparation, raw material pretreatment, ingredient mixing, fermentation inoculant inoculation, sealed fermentation, fermentation post-treatment and effect verification optimization, by optimizing the production process of the grain amaranth fermented feed, the nutritional value and palatability of the feed are improved, it is ensured that antinutritional factors of grain amaranth are effectively degraded, and the yield of the grain amaranth is improved. Key nutritional ingredients in the raw materials are fully reserved and efficiently absorbed by pigs, the feed intake, the weight gain rate and the feed conversion rate of the pigs are remarkably increased, the content of crude fibers is reduced, the availability of nutrient substances is enhanced, the flavor and the texture of pork are improved, lactic acid bacteria are efficiently utilized and fermentation conditions are accurately controlled in the fermentation process, and the production cost is reduced. The residues of undigested protein and cellulose in the pig manure are reduced, the emission of harmful gases such as ammonia gas and hydrogen sulfide is reduced, and the air quality and sanitary conditions of a farm are improved.

The invention belongs to the field of microorganisms, and particularly relates to bacillus Z161 capable of efficiently degrading straw as well as a screening method and application of the bacillus Z161. The bacillus subtilis Z161 is bacillus mexicanus, the preservation number of the bacillus mexicanus Z161 is CGMCC (China General Microbiological Culture Collection Center) NO.36393, and the bacillus mexicanus Z161 is preserved in the China General Microbiological Culture Collection Center on October 29, 2025. The bacillus Z161 screened by the invention has strong laccase activity and high temperature resistance, and can efficiently degrade lignin under the condition of 50 DEG C; the problem that in the prior art, normal-temperature zymophyte is mostly adopted, and the requirement for fermentation conditions in the fermentation process is harsh is solved, and therefore the rapid compost fermentation technology is easier to achieve. The bacillus Z161 provided by the invention has the advantages of genetic and functional character stability, has good tolerance at high temperature, enables lignin to be quickly depolymerized, and improves the decomposition efficiency.

The invention discloses a probiotic solid fermentation material turning device which comprises a fermentation tank and a base arranged below the fermentation tank, the two ends of the top of the base are fixedly connected with supports through supporting frames, and the supports are fixedly installed at the bottom of the outer side of the fermentation tank. According to the invention, a double-spiral stirring structure is arranged, and two spiral stirring shafts with opposite rotating directions synchronously and reversely rotate, so that continuous and uniform stirring of materials can be ensured, and heat can be quickly dissipated; meanwhile, an annular conveying belt type material turning mechanism is arranged, so that the materials are continuously lifted and thrown down by a material turning plate, the continuous turning of the materials can be realized, the materials are ensured to be turned more fully in the fermentation process, and the material turning effect is better; and the stirring shaft is designed into a hollow structure, hot water or cold water is introduced as required, the temperature of the materials is flexibly regulated and controlled, and the fermentation conditions are comprehensively optimized so as to ensure that the solid fermentation effect of the probiotics is better.

The invention provides a genetically engineered bacterium for producing bacterial cellulose as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of bioengineering. According to the invention, a K.medellensis expression vector is constructed, a glycerol transporter expression vector and a glycerol kinase expression vector are further constructed based on the K.medellensis expression vector, and the K.medellensis glycerol transporter expression vector and the glycerol kinase expression vector are transformed into K.medellensis to construct a genetically engineered bacterium for producing bacterial cellulose; glycerin and corn steep liquor are directly used as a carbon source and a nitrogen source respectively, and the fermentation conditions of the genetically engineered bacteria are further optimized, so that environment-friendly and low-cost synthesis of the bacterial cellulose is realized, the yield of the bacterial cellulose is remarkably increased, and the method has good practicability.

The invention discloses a glucose oxidase mutant with improved acid resistance and heat resistance as well as a construction method and application of the glucose oxidase mutant, and belongs to the technical field of bioengineering. According to the present invention, glucose oxidase AsGODP derived from Aspergillus scleroticus is adopted as a female parent, and Glu148 and Val179 are subjected to synchronous mutation to form Lys148 and Ala179 so as to obtain the mutant E148K / V179A; experiments show that the pH stability, the thermal stability, the half-life period at 75 DEG C and the catalytic performance of the mutant E148K / V179A are obviously improved compared with those of wild AsGODP. According to the catalytic performance, when the mutant E148K / V179A is applied to bread baking, the gluten degree of dough can be effectively improved under the fermentation condition, the bread texture is improved, and therefore the mutant E148K / V179A shows huge development potential in the bread baking industry.

The invention discloses a high-yield flower and fruit aroma strain, culture thereof and application of the high-yield flower and fruit aroma strain in sauce-flavor food brewing, the strain is named as Wickerhamomyces anomalus GZZJ2023Y053, and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 2025875, and the preservation number of the strain is CCTCC NO: M 2025875. The preservation date is April 24, 2025; the preservation address is Wuhan University, Wuhan, China. The culture method of Wickerhamomyces anomalus comprises the following steps: inoculating activated Wickerhamomyces anomalus GZZJ2023Y053 into a liquid culture medium, and fermenting at constant temperature to obtain a seed solution; the invention also provides an application of the Wickerhamomyces anomalus GZZJ2023Y053 in preparation of ethyl acetate and brewing of sauce-flavor foods. According to the method, multiple Wickerhamomyces anomalus separated from fermented grains is subjected to separation culture, the content of ethyl acetate is measured after fermentation is finished, then the screened Wickerhamomyces anomalus GZZJ2023Y053 has the capacity of producing ethyl acetate at high yield, and after fermentation conditions are further optimized, the yield of the ethyl acetate is improved. The capability of synthesizing ethyl acetate is greatly improved.

The invention discloses a preparation method and application of a novel bird nest alkane diterpenoid compound, and relates to the technical field of medicinal chemistry. The invention aims to solve the problems that chemical synthesis needs harsh conditions and the cost is difficult to control when diterpenoid compounds are obtained in the prior art, and a large amount of resources are wasted and new molecules are difficult to obtain under conventional fermentation conditions. The prepared bird nest alkane type diterpene has dual functions of promoting neurite growth and nourishing neurons, is expected to be developed into a neuroprotective agent, and is used for treating neurodegenerative diseases and peripheral nerve injury diseases; meanwhile, the problems that in the prior art, when the compounds are obtained, conditions needed by chemical synthesis are harsh, the cost is difficult to control, a large number of resources are wasted under conventional fermentation conditions, but new molecules are difficult to obtain, and production and smooth follow-up biological activity research are not facilitated are effectively solved. The invention provides the preparation method and the application of the novel bird nest alkane diterpenoid compound.

The invention discloses a method for culturing seawater rotifers by using prawn shell leavening, which comprises the following steps: S1, cleaning prawn shells to obtain a fermentation substrate; s2, dynamically optimizing fermentation conditions by adopting a multi-agent collaborative reinforcement learning algorithm; s3, predicting the optimal completion time of fermentation by adopting a mixed graph convolutional neural network and an adaptive genetic algorithm, and providing dilution suggestions through a spectral analysis technology; s4, dynamically calculating the dilution ratio, and mixing the fermentation liquor with seawater; s5, pouring the diluent into a rotifer culture pond, predicting the health state of the rotifer by adopting a Vision Transform model, and automatically adjusting a nutrition formula or environmental conditions; s6, monitoring the density of the rotifers in real time through a YOLOv5 target detection model and a camera, and keeping the rotifers within a preset density range; and S7, performing intelligent management on waste treatment based on a multi-objective evolutionary algorithm of non-dominated sorting. According to the method, a multi-agent collaborative algorithm and a deep learning technology are adopted, and intelligent culture of seawater rotifers and optimized treatment of wastes are realized.

The invention belongs to the field of fermentation strain screening, and discloses a high-throughput fermentation strain screening method based on artificial intelligence. Comprising the following steps: acquiring standard strains from a public strain preservation center, separating potential functional strains from edible mushroom residues, utilizing a high-throughput fermentation platform, setting different fermentation conditions, monitoring physical parameters in real time through an online sensor, constructing an artificial intelligence model, and quickly screening out dominant strains through real-time data acquisition and model prediction. Designing an optimization experiment scheme by using an AI performance prediction model, and according to a high-throughput experiment result, carrying out multi-round iterative optimization to obtain a single strain or a composite flora with optimal performance; according to the method, actual edible fungus residues are used as fermentation substrates, multiple groups of repeated experiments are set, strains or composite flora with the optimal performance are screened out, and intelligent screening of high-throughput fermentation strains is achieved.

The invention belongs to the technical field of brewing microbial fermentation, and particularly relates to a new monascus purpureus strain and application thereof. In order to further enrich beneficial microorganisms in a white spirit brewing environment, the invention provides a new Monascus purpureus strain capable of producing protease and hydrolase at high yield, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41533. The strain is used for preparing mouldy bran, yeast for making hard liquor and strengthened yeast for making hard liquor, and various hydrolases for brewing can be produced at high yield; and various flavor substances such as phenethyl alcohol, isoamyl alcohol and phenylacetaldehyde can be produced under the simulated pit fermentation condition, so that the requirements of brewing of multi-grain Luzhou-flavor liquor are met, a new choice is provided for improving the flavor of liquor in liquor brewing, and the method has wide application prospects and great application value.

The invention discloses a recombinant engineering bacterium for expressing fetuB and application of the recombinant engineering bacterium, and relates to the technical field of genetic engineering. According to the recombinant engineering bacteria, pichia pastoris serves as host bacteria, after fetuB genes are introduced, efficient expression of the fetuB in the pichia pastoris is achieved by optimizing codons and improving fermentation conditions, and the expression quantities of CBS7435 under the shake-flask culture condition are 320 + / -25 mg / L, SMD116 under the shake-flask culture condition is 280 + / -30 mg / L, and SuperMan5 under the shake-flask culture condition is 580 + / -45 mg / L. After fermentation conditions are optimized, the culture yield of a fermentation tank can reach 27.35 g / L. The fetuin B disclosed by the invention is good in biological activity, high in expression, simple in operation process and suitable for large-scale industrial production.

The invention provides a post-treatment method of single-cell protein fermentation liquor and a fermentation method of single-cell protein thalli. Nuclease and cell wall lytic enzyme are respectively added into fermentation liquor containing single-cell protein thalli to treat the thalli, so that nucleic acid and cell wall residues can be reduced, and the problems that in a traditional fermentation process, the application of the thalli in food is limited due to the high nucleic acid content of the thalli, and the protein extraction efficiency is reduced due to the existence of the cell walls are solved. In addition, by optimizing fermentation conditions, the protein content of the single-cell protein is remarkably increased. The process is simple, low in cost, suitable for industrial production and capable of being widely applied to the fields of feed, food and medicine.