79 results about "Aflatoxin degradation" patented technology

The invention discloses a method for degrading aflatoxin, which makes use of gamma rays to irradiate a sample containing the aflatoxin, wherein the gamma rays are generated by a radioactive substance Co, and the irradiation dose is between 0 and 10 kGy not including 0, preferably between 2 and 10 kGy, more preferably between 4 and 10 kGy, particularly preferably between 6 and 10 kGy, and the most preferably 10 kGy. The sample containing the aflatoxin is a farm product containing the aflatoxin or a product obtained by processing the farm product, such as food, feedstuff, and the like. The method is particularly applicable to degrading the aflatoxin B1, not only can kill pathogenic microorganisms but also can degrade biotoxin therein, and does not generate any industrial pollution.

The invention provides Lysobacter capable of efficiently degrading aflatoxin B1 and ochratoxin A and application of the Lysobacter and particularly provides double-function Lysobacter sp. CW239 and application thereof to the degradation of low-pollution concentration aflatoxin B1 and ochratoxin A. Compared with existing aflatoxin degrading bacteria, the Lysobacter sp. CW239 has the advantages that the Lysobacter sp. CW239 can achieve excellent degrading effect under a low-concentration toxin pollution condition; under a liquid fermentation condition, in fermentation broth, which contains the aflatoxin B1 and ochratoxin A, with the final concentration of 20 microgram/L, the 12-hour ochratoxin A degradation rate of the Lysobacter sp. CW239 is 53.1%, and the 48-hour degradation rate reaches 99.8%; the 12-hour aflatoxin B1 degradation rate of the Lysobacter sp. CW239 is 42.5%, and the 48-hour degradation rate reaches 83.4%; when the Lysobacter sp. CW239 is used for processing feed (with the final concentration of 20 microgram/kg) polluted by toxins, the 48-hour ochratoxin A degradation rate is 68.7%, and the 48-hour aflatoxin B1 degradation rate is 52.1%; the Lysobacter sp. CW239 has substantial application value and significance when being applied to food and feed bio-detoxification.

The invention provides luteimonas sp for degrading alflatoxin B1 and ochratoxin A and application of the luteimonas sp and particularly provides a strain of difunctional efficient degrading bacteria (Luteimonas sp.) CW574 and application thereof in degrading low-pollution-concentration alflatoxin B1 and ochratoxin A. Compared with existing aflatoxin degrading bacteria, the strain CW574 can achieve excellent degrading effects under the condition of low-concentration toxin pollution. Under the condition of liquid fermentation, in a fermentation culture solution containing alflatoxin B1 and ochratoxin A with the final concentration being 20 micrograms per liter, the degrading rate of the strain CW574 on ochratoxin A is 90.1% in 48 h; the degrading rate of the strain CW574 on alflatoxin B1 is 85.5% in 48 h. When the strain CW574 is used for treating fodder (the final concentration is 20 micrograms per kg) polluted by toxins, the degrading rate on OTA is 48.3% in 48 h, and the degrading rate on AFB1 is 52.1% in 48 h. The strain CW574 has substantive application value and significance on the application aspects of food and feed biological detoxification.

The invention discloses solimonas capable of efficiently degrading aflatoxin B1 and application thereof, and particularly provides a solimonas (solimonas sp.) strain CW980 and application thereof in degradation of aflatoxin B1 with the low pollution concentration. Compared with an existing aflatoxin degradation bacterium, the strain CW980 can achieve the excellent degradation effect under the low-concentration aflatoxin pollution condition. Under the liquid fermentation condition, in a fermentation culture solution containing the aflatoxin B1 with the final concentration of 20 mug/L, the degradation rate of the strain CW980 to the aflatoxin B1 after 24 h is 37.8%, the degradation rate after 48 h reaches 70.3%, and the degradation rate after 72 h reaches up to 90.4 %. By processing feed polluted by the aflatoxin B1(the final concentration is 20 mug/kg) with the strain CW980 for 48 h, the AFB1 degradation rate is 23.0%, and the AFB1 degradation rate reaches 75.5% after 72 h. The strain CW980 has the substantial application value and great significance in the aspect of biological detoxification application of food and feed.

The invention discloses a Pleurotus ostreatus and its application in aflatoxin degradation. The Pleurotus ostreatus LY65 provided by the invention is called LY65 for short, and has a preservation number of CGMCC No.5428. A broth of the LY65 can be used for degrading aflatoxin B1, has a high degradation capability, and is nontoxic and innocuous to humans and animals because LY65 strains are edible strains. LY65 and its broth have good application prospects in the aflatoxin B1 degradation.

The invention discloses an arthrobacterium and an application thereof in degradation of aflatoxin and provides an arthrobacterium (Arthrobacter sp.) L15 which is assigned the accession number CGMCC No.8869. An experiment in the invention proves that the arthrobacterium L15 is discovered in the invention. Aflatoxin can be degraded efficiently when a fermentation liquor of the arthrobacterium L15 is added to the aflatoxin and dissolving and uniform mixing are carried out. The arthrobacterium L15, used as a biological material for degrading aflatoxin, has good application prospects in exploitation of not only novel biodegradation inoculants but also novel biodegradation sterile preparations.

The invention discloses a mycotoxin adsorption degradation agent for pig feed and a preparation method thereof. The mycotoxin adsorption degradation agent is prepared from the following components inparts by weight: 15 to 25 parts of activated lactic acid bacteria, 15 to 25 parts of bacillus subtilis, 20 to 40 parts of saccharomycetes, 20 to 30 parts of lactic acid bacterium cell wall peptidoglycan, 2 to 6 parts of an aflatoxin degeneration enzyme, 2 to 6 parts of a zearalenone degeneration enzyme, 2 to 6 parts of a vomitoxin degeneration enzyme, 2 to 6 parts of an ochratoxin degeneration enzyme, 2 to 6 parts of a T-2 toxin degeneration enzyme, 100 to 200 parts of hydrated aluminosilicate, 10 to 20 parts of potassium sorbate, 10 to 20 parts of sodium benzoate, 25 to 40 parts of pericarpium citri reticulatae, 25 to 40 parts of fructus crataegi, 25 to 35 parts of cortex cinnamomi and 10 to 25 parts of flos caryophylli. The adsorption degradation agent is subjected to biological adsorption and intestinal degradation, so that mycotoxin in raw materials of the feed and in the feed can be effectively removed and the health and safety of live pigs are ensured.

The present invention provides aflatoxin degrading enzyme secreted by Bacillus subtilis, and applications thereof. According to the present invention, the aflatoxin degrading enzyme BADE is firstly separated and purified from Bacillus subtilis ANSB060, wherein the amino acid sequence is represented by SEQ ID NO:1, and the gene encoding the enzyme is obtained through cloning; the gene is transformed into an Escherichia coli expression system so as to successfully achieve the expression of the protein in Escherichia coli; and the activity identification results prove that the recombinant rBADE has aflatoxin degrading effect, such that the foundation is established for the development of the aflatoxin degrading enzyme with high specificity and high transformation efficiency.

The invention discloses a microbial fermentation based aflatoxin degradation device. The microbial fermentation based aflatoxin degradation device comprises a body and is characterized in that the body is internally provided with a mixing cavity, and a mixing mechanism is arranged in the mixing cavity and capable of well mixing feed with degradation mixed strains to facilitate the next fermentation step. The body on the right side of the mixing cavity is internally provided with a fermentation cavity, and a fermentation mechanism is arranged in the fermentation cavity and enables complete fermentation of the feed with degradation bacteria to further enable complete fermentation degradation of aflatoxin in the feed. The body at the bottom of the mixing cavity is internally provided with a slide cavity, and a power mechanism is arranged in the slide cavity. The aflatoxin degradation device has advantages that by a mixing device for well mixing the feed with the mixed strains, convenience in fermentation is achieved; under the stirring action of second stirring plates and the heating action of a heating layer, the fermentation speed is increased, and time saving is realized.

The invention relates to an aflatoxin degradation method, in particular to an aflatoxin irradiation degradation method. The aflatoxin irradiation degradation method comprises the steps that an asporgillus flavus spore suspension is prepared; an experimental agricultural product is placed under an ultraviolet lamp to be subjected to irradiation sterilization, and the sterilized agricultural product is placed into the asporgillus flavus spore suspension to be mixed evenly and cultured, so that the experimental agricultural product infected with asporgillus flavus is obtained; the experimental agricultural product infected with the asporgillus flavus is placed into alcohol with the volume concentration being 80-85% to be soaked and stirred, and heated in a water bath with the temperature being 70-80 DEG C, and the experimental product which is infected with the asporgillus flavus and degraded preliminarily is obtained; and the experimental agricultural product infected with the asporgillus flavus is placed into a centrifugal tube to be sealed, and the sealed experimental agricultural product infected with the asporgillus flavus is placed into a <60>Co gamma ray radiation field to be irradiated and then placed into an electron beam radiation field to be irradiated. By adoption of the aflatoxin irradiation degradation method, the degradation rate of aflatoxin in the agricultural product is increased, the degrading effect is improved, industrial pollution is reduced, and cost allowance of enterprises in the production process is reduced.

The invention discloses a feed additive capable of decomposing mycotoxin and a preparation method thereof. The feed additive is prepared from the following components in parts by weight: 20 to 40 parts of mannan oligosaccharides, 15 to 25 parts of activated lactobacillus, 15 to 25 parts of bacillus subtilis, 20 to 30 parts of lycopene, 2 to 6 parts of aflatoxin degrading enzyme, 2 to 6 parts of zearalenone degrading enzyme, 2 to 6 parts of vomitoxin degrading enzyme, 2 to 6 parts of selenium yeast, 2 to 6 parts of polyglutamic acid, 100 to 200 parts of hydrated silicon aluminate, 10 to 20 parts of flos sophorae immaturus, 10 to 20 parts of herba taraxaci, 25 to 40 parts of pericarpium citri reticulatae, 25 to 40 parts of radix astragali, 25 to 35 parts of cassia bark and 10 to 25 parts offlos caryophylli. According to the feed additive capable of decomposing mycotoxin disclosed by the invention, through biological enteral degradation, mycotoxin in raw materials of the feed and the feed can be effectively removed, and health and safety of live pigs are ensured.

The invention discloses Bacillus subtilis for efficiently degrading aflatoxin and application thereof, and belongs to the field of biotechnology. The Bacillus subtilis is named ASAG212, and was preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 18367. The optimum degradation conditions are as follows: the initial concentration of Bacillus subtilis is 109 CFU/mL, the pH value is 7.2-7.6, the culture temperature is 35-40 DEG C, and the rotational speed is 120 rpm-180 rpm. 10 ug/mL of aflatoxin B1 is degrade into a non-toxic productwithin 4 hours by the strain of the invention, and the degradation rate reaches more than 90%. The invention also provides a bacterial preparation containing the strain and for degrading aflatoxin andan application of the bacterial preparation. The preparation method of the bacterial preparation used by the invention is simple, and the bacterial preparation does not contain antibiotic components,has no toxic or side effects, has no drug resistance, has no pollution, and has remarkable economic benefits and practical application value.

The invention provides a capsule rhodobacter strain for degrading aspergillus flavus B1, application of the capsule rhodobacter strain, a degradation agent and application of the degradation agent, belonging to the technical field of degradation of aflatoxin. The provided capsule rhodobacter strain for degrading the aspergillus flavus B1 is preserved in China General Microbiological Culture Collection Center and has a preservation number of CGMCC No.14603. The capsule rhodobacter strain for degrading aspergillus flavus B1 is applied to the degradation of the aspergillus flavus B1. The aspergillus flavus B1 degradation agent for animal feeds contains the capsule rhodobacter strain. The aspergillus flavus B1 degradation agent is applied to the preparation of the animal feeds. The degradation agent has the properties of the efficient aspergillus flavus B1, and the nutrient contents of crude proteins, true proteins, crude fat and the like in the animal feeds are remarkably increased, so that the feeding value of the animal feeds is greatly increased.

The invention provides a composite probiotic fermentation composition and application thereof in preparation of a preparation for relieving epithelial cell damage caused by mycotoxin, and belongs to the technical field of mycotoxin degradation. The composite probiotic fermentation composition comprises a compound probiotic fermentation supernatant and an aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzyme solution, wherein the volume ratio of the compound probiotic fermentation supernatant and the aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzymesolution is (2.6 to 3.4) : (1.5 to 2.5). The aflatoxin B1 degrading enzyme activity of the aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzyme solution is 250-350 U/L, and thezearalenone degrading enzyme activity of the aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzyme solution is 25-35 U/L. The composite probiotic fermentation composition can effectively relieve epithelial cell damage caused by aflatoxin B1 (AFB1) and zearalenone (ZEA).

The invention discloses a method for degrading aflatoxins in peanut meal by utilizing solid fermentation of fructificatio amaurodermatis rudae. The method comprises the steps of treating raw materials, activating strains, performing solid fermentation, degrading the aflatoxins, determining the aflatoxins and the like. The invention provides an efficient safe method for degrading the aflatoxins in the peanut meal. The degraded peanut meal can be used for producing and processing foods and feeds, and besides, the nutrient value of the peanut meal can also be increased. The method is simple to operate and easy in industrialization.

The invention provides a trichoderma.citrinoviride strain XZ0509 and application thereof, and relates to the technical field of aflatoxin degradation. The preservation number of the strain XZ0509 is CCTCC M 2020522. The invention further provides the application of the strain XZ0509 in degradation of aflatoxin B1. After the strain XZ0509 is cultured for 3 days, the rate of degradation on an AFB1 solution with the concentration of 50ppt is greater than 80%, after the strain XZ0509 is cultured for 7 days, the AFB1 removal rate is 100%, and after the strain XZ0509 is cultured for 5 days, the rateof degradation on 10ppm AFB1 is still higher than 50%. The strain has strong bacteriostatic and toxin-producing-inhibiting effects at the same time, the inhibition rate on aspergillus flavus hypha is85.6%, and the rate of inhibition on toxin production of aspergillus flavus in peanuts is 92.8%. It is shown that the strain XZ0509 can be applied to degradation of aflatoxin in seriously polluted grain and oil products.

The invention discloses a degradation agent of aflatoxin, a preparation method and application thereof, and belongs to the technical field of aflatoxin degradation. The degradation agent of the aflatoxin is prepared from the following components: 60-100 g of fresh aloe, 75-125 g of fermentation liquor of fresh lemon, 1% of oleum menthae, 0.1-0.5% of laccase, and 0.2% of Tween-80. Through the mutual coordination among the components, the aflatoxin is adequately degraded. The degradation agent of the aflatoxin is safe in aflatoxin degradation, high-efficient, and mild in reaction conditions, andis capable of degrading the aflatoxin by 96% or above.