17 results about "Aflatoxin degradation" patented technology

The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.

This utility model discloses a peanut oil aflatoxin degradation machine, including a casing with an oil inlet and an oil outlet. The casing also houses an oil inlet pump, an oil pan, a weighing device, an oil outlet pump, an ultraviolet lamp, and a control unit. The oil pan has an oil inlet end and an oil outlet end. The oil inlet pump is connected to the oil inlet end of the oil pan via an oil delivery pipe. The oil outlet end of the oil pan is connected to the weighing device via an oil delivery pipe. The oil outlet pump is connected to the weighing device and the oil outlet end via an oil delivery pipe. The ultraviolet lamp is used to sterilize and disinfect the peanut oil in the oil pan. The oil inlet pump, ultraviolet lamp, weighing device, and oil outlet pump are all electrically connected to the control unit. This utility model uses ultraviolet lamps to sterilize and disinfect peanut oil, achieving efficient degradation of aflatoxin in peanut oil without introducing chemical residues, ensuring the quality and safety of peanut oil for consumption. It is simple to operate, has high processing efficiency, and low cost.

The invention belongs to the technical field of biological mildew preventive, and discloses bacillus licheniformis fermentation liquor and application thereof in mildew prevention and quality improvement of silage. The fermentation liquor is prepared by taking bacillus licheniformis CMCC 63516 as a strain under optimized conditions (with an LB culture medium, the inoculum size of 6%, the liquid loading amount of 30% and culture for 18 hours), and the viable count can reach 1.48 * 10 < 9 > CFU / mL. The strain has a remarkable antagonistic effect on toxin-producing aspergillus flavus, can efficiently degrade aflatoxin B1 (the degradation rate gt in 36 hours is 96%), and has good environmental stability. The additive amount of 1.2-4.0% is applied to corn silage, the growth of mould and saccharomycetes can be remarkably inhibited, the content of AFB1 is greatly reduced, and the mould-proof effect is superior to that of a chemical mould-proof agent sodium diacetate. In addition, the fermentation liquor can also effectively improve the quality of silage, increase the content of crude protein and lactic acid, and reduce the pH and the content of ammoniacal nitrogen and detergent fibers. The invention provides a safe, environment-friendly and efficient biological mildew-proof method which has a wide application prospect in the feed industry.

The application discloses a method for degrading aflatoxin by using bacillus, 5ml of bacterial liquid is transferred into a sterilized glass tube, aflatoxin standard B1 and G1 are added, and the final concentration is adjusted, meanwhile, a blank medium with standard samples is used as a control, and culture is carried out at 37 DEG C on a shaking table; centrifugal and filtration treatment are carried out on the sample, and the treated sample is detected on a machine; S2: the degradation product of bacillus 21-1-2 for degrading aflatoxin is identified by using high performance liquid chromatography-high resolution mass spectrometry combined with UNIFI 1.8.2 / Masslynx V 4.1 software, and the final identification result is obtained; the method adopts real-time external standard method mass number correction technology, ensures high accuracy of the mass number, and has small mass number deviation; the MSE full information tandem mass spectrometry data acquisition mode has no content discrimination, ensures full acquisition of information, acquisition of fragment ions of low response compounds, and simultaneously ensures accuracy of the mass number of parent ions and fragment ions; the metabolite identification result is that bacillus 21-1-2 can degrade Alfatoxin G1 into Aflatoxin G1+CH2CH2-C3H2O.

The invention discloses an aflatoxin degrading enzyme mutant and application thereof, and belongs to the technical field of enzyme engineering and microorganisms. In order to solve the technical problems that an existing aflatoxin B1 degrading enzyme mostly depends on a mild operation environment, the degradation performance is poor in a high-temperature environment or an extreme acid-base condition, the application range of the aflatoxin B1 degrading enzyme is remarkably limited and the like, the invention provides an aflatoxin B1 degrading enzyme mutant which can efficiently and rapidly degrade aflatoxin B1, and the aflatoxin B1 degrading enzyme mutant has the advantages that the aflatoxin B1 degrading enzyme mutant has a good application prospect. And the strain also has the characteristics of acid and alkali resistance and high temperature resistance, shows excellent performance in the aspect of aflatoxin B1 pollution treatment, and has a good industrial application prospect. Besides, food-grade safe strains such as saccharomyces cerevisiae, bacillus licheniformis or bacillus subtilis are used as host strains for preparing the aflatoxin B1 degrading enzyme mutant, the degrading effect can be guaranteed, meanwhile, the higher requirement for the safety of enzyme preparations in the feed field can be met, and the application of the aflatoxin B1 degrading enzyme mutant has more advantages.

The invention discloses a composite protective agent for liquid laccase and application of the composite protective agent, and belongs to the technical field of bioengineering. The protective agent takes a citric acid-disodium hydrogen phosphate buffer solution with the pH value of 4.5-6.5 as a solvent, and comprises propylene glycol, glycerol, sorbitol, cane sugar, maltodextrin, glycine, trehalose, potassium sorbate, diacetic acid and a mediator. The components form a stable microenvironment around enzyme molecules through a synergistic effect, the polyhydric alcohols and the saccharides stabilize an enzyme structure through hydrogen bonds and glassy state protection, the glycine plays a buffering role, and the mediator has dual functions of oxidation resistance and mediated catalytic reaction. Experiments prove that the protective agent can remarkably improve the thermal stability and storage stability of the liquid laccase, the enzyme activity retention rate reaches 86.7% after the liquid laccase is treated at 50 DEG C for 24 h, and the enzyme activity retention rate is still kept at 85.3% after the liquid laccase is stored at 37 DEG C for 180 days. The laccase is especially suitable for degrading aflatoxin B1, has a degradation effect obviously better than that of unprotected laccase, and can be widely applied to the fields of food industry, feed industry and the like.

The application provides a construction method of aflatoxin B1 degrading enzyme, which takes original aflatoxin B1 degrading enzyme as a starting enzyme, only mutates alanine at the 367th position of the original aflatoxin B1 degrading enzyme into glutamic acid, glutamic acid at the 445th position into lysine, isoleucine at the 477th position into glutamic acid, or only mutates threonine at the 258th position of the original aflatoxin B1 degrading enzyme into alanine, alanine at the 367th position into glutamic acid, glutamic acid at the 445th position into lysine, isoleucine at the 477th position into glutamic acid, and the amino acid sequence of the original aflatoxin B1 degrading enzyme is shown in SEQ ID NO:1; and the method of the application utilizes a combined mutation method to improve the specific enzyme activity of the aflatoxin B1 degrading enzyme without the need of an electron donor.

The invention relates to the technical field of aflatoxin degradation, in particular to TC degradation liquid and application thereof to degradation of aflatoxin B1 in agricultural products. The TC degradation liquid provided by the invention is prepared from trametes cochinea (Trametes cochinea), and the TC degradation liquid provided by the invention is prepared from the trametes cochinea. According to the method for degrading the aflatoxin B1 in the agricultural product, the TC degradation liquid is sprayed to the agricultural product containing the aflatoxin B1. According to the method for degrading the aflatoxin B1 in the agricultural products, provided by the invention, only the prepared degradation liquid needs to be simply sprayed under a definite dosage and reacts under a mild temperature condition (room temperature), the operation is simple and convenient, the reaction condition is mild, the energy consumption is low, and meanwhile, the damage to the original quality of the agricultural products is avoided.

The invention discloses a method for degrading aflatoxin by magnetic nanoparticle immobilized biological enzyme, and belongs to the technical field of immobilized biological enzyme. The method comprises the following steps: preparing aminated ferroferric oxide magnetic nanoparticles (Fe3O4 (at) NH2) through a chemical coprecipitation method, and carrying out functional modification on the surfaces of the magnetic nanoparticles by adopting 3-aminopropyltriethoxysilane to obtain a magnetic carrier; the aflatoxin degrading enzyme is covalently bound to the surface of the magnetic carrier through a glutaraldehyde cross-linking method, and finally the magnetic nanoparticle immobilized biological enzyme with high stability and recoverability is obtained. The method solves the problems that the enzyme is easy to fall off and difficult to recover in a traditional enzyme immobilization method, the stability and reuse efficiency of the enzyme in an organic solvent are remarkably improved, and the method is suitable for efficient degradation of aflatoxin in food and feed.

The invention provides a construction method of an aflatoxin B1 degrading enzyme, which comprises the following steps: taking an original aflatoxin B1 degrading enzyme as a starting enzyme; only 367-site alanine of the original aflatoxin B1 degrading enzyme is mutated into glutamic acid, 445-site glutamic acid of the original aflatoxin B1 degrading enzyme is mutated into lysine, and 477-site isoleucine of the original aflatoxin B1 degrading enzyme is mutated into glutamic acid, or only 258-site threonine of the original aflatoxin B1 degrading enzyme is mutated into alanine, 367-site alanine of the original aflatoxin B1 degrading enzyme is mutated into glutamic acid, and 445-site glutamic acid of the original aflatoxin B1 degrading enzyme is mutated into lysine. The 477 isoleucine is mutated into glutamic acid, and the amino acid sequence of the original aflatoxin B1 degrading enzyme is as shown in SEQ ID NO: 1; by utilizing a combined mutation method, the specific enzyme activity of the aflatoxin B1 degrading enzyme is improved under the condition that an electron donor is not needed.

The invention discloses a nano-enzyme with laccase activity and coexistence of Cu single atoms and Cu nano-particles. The nano-enzyme is used for degrading aflatoxin B1. Cu nanoparticles are loaded on Cu single atoms through a one-step pyrolysis method, CuSAC and CuNPs are successfully synthesized, under the low concentration of the material, the CuSAC and CuNPs can rapidly generate free radicals to oxidize AFB1, the degradation rate of aflatoxin B1 within 30 min can reach 90% or above, meanwhile, the material has good anti-interference performance and stability, and experiments show that the CuSAC and CuNPs nano-enzyme has huge potential in the aspect of degrading aflatoxin B1.

The invention discloses a preparation method of a candida utilis biological feed and application of the candida utilis biological feed to piglets, and belongs to the technical field of biological agriculture. The method comprises the following steps: (1) pretreatment: mixing a bacterial liquid containing candida utilis saccharomycetes with a modified carrier according to a mass ratio of 1: (0.05-0.2) to obtain a fermentation substrate; (2) nutrient enrichment treatment: adding corn powder and soybean powder, and performing shaking table fermentation at 30-37 DEG C for 18-24 hours to obtain primary fermentation liquor; (3) fermentation microenvironment treatment: adding cellulose and an iron-based material, stirring for 30-60 minutes at the temperature of 30-34 DEG C and the pH value of 5-6, and maintaining the dissolved oxygen to be 1-1.2 mg / L; (4) fermentation regulation and control treatment: monitoring pH and aflatoxin degradation rate in real time to obtain final fermentation liquor; and (5) drying and packaging: performing spray drying to obtain the biological feed. According to the method, specific strains and modified carriers are utilized, the yeast attachment and fermentation efficiency is improved, aflatoxin can be effectively degraded (the degradation rate is 84.3%-86.7%), the protein content of the feed is increased (24.6%-26.5%), and the fermentation efficiency reaches 74.7%-77.1%.

The utility model discloses an edible vegetable oil aflatoxin degradation device which comprises a tank body, a first partition plate, a second partition plate and a third partition plate are sequentially fixed in the tank body from bottom to top, and the interior of the tank body is divided into a bottom cavity, a cooling cavity and a top cavity by the first partition plate, the second partition plate and the third partition plate. A feeding pipe is fixedly connected to the top end of the side wall of the top cavity, and a discharging pipe is fixed to the bottom wall of the bottom cavity. According to the utility model, the tank body is arranged, the interior of the tank body is divided into the bottom cavity, the cooling cavity and the top cavity, and when the ultraviolet lamp irradiates vegetable oil in the top cavity, the food pump circularly pumps the oil in the bottom cavity into the top cavity, so that the vegetable oil in the bottom cavity and the top cavity continuously exchanges positions; meanwhile, the motor is matched to drive the stirring blades to rotate to stir the vegetable oil, it is guaranteed that the ultraviolet lamp can fully and evenly irradiate the vegetable oil at each position, and then the aflatoxin degradation effect is improved.

This invention provides the use of aflatoxin-degrading enzymes, recombinant plasmids, and recombinant bacteria in the preparation of drugs that degrade aflatoxin, belonging to the field of biotechnology. The aflatoxin-degrading enzymes of this invention are extracted from Bacillus amyloliquefaciens strain 906, and their amino acid sequences are shown in SEQ ID NO.1 or SEQ ID NO.11. The two novel aflatoxin-degrading enzymes extracted from Bacillus amyloliquefaciens strain 906 exhibit excellent degradation effects on aflatoxin B1 and have promising application prospects.

The invention discloses a microbial agent for degrading aflatoxin and a preparation method thereof. The microbial inoculum takes bacillus velezensis and aflatoxin degrading enzyme secreted by the bacillus velezensis as core degradation components, and functional synergy and stability protection are realized through a structured microcapsule. Mannan-oligosaccharide and serum albumin are added to the outer layer of the microcapsule, and toxin enrichment is achieved by means of high affinity of mannan-oligosaccharide and serum albumin to aflatoxin; main components of the microcapsule are chitosan and kappa-carrageenan, and the microcapsule is endowed with a more efficient chemical oxidative degradation function by grafting TEMPO with chitosan. Meanwhile, the chitosan component has bacteriostatic activity, can inhibit growth and metabolism of aspergillus flavus and aspergillus parasiticus, and reduces toxin neogenesis. According to the degradation microbial agent, through the synergistic effect of toxin enrichment, chemical oxidative degradation and biological specific degradation, the degradation efficiency and action durability of aflatoxin are remarkably improved, the stability and targeting property of the core degradation component are guaranteed through the microcapsule structure, and the application prospect is wide.

The application of a copper-based nanomaterial with laccase-like activity in aflatoxin B1 degradation, the particle size of Cu-NADH nanoparticles is 10-50 nm, copper ions and NADH form the basic structural unit of nanoparticles through Cu-N coordination, and extend through Cu-N-P coordination to form a chain structure with a length of more than 1 mu m, and the whole presents a loose and porous network structure, which can provide a larger specific surface area for enzymatic reaction. The copper-based nanomaterial with laccase-like activity is applied in aflatoxin B1 degradation; the application has the characteristics of high degradation efficiency and low cost.

The invention discloses an integrated small oil workshop safety production management system and method based on a cloud platform, and relates to the technical field of edible oil safety production, the integrated small oil workshop safety production management system comprises a management end, a cloud server and a production end, the management end and the production end are in network communication with the cloud server, and the cloud server sends received production information to the management end; the production end comprises aflatoxin degradation machines and oil pressing equipment which are arranged in small oil workshops, various production information after the aflatoxin degradation machines and the oil pressing equipment run is sent to the cloud server through a communication module, the cloud server sends the received production information to the management end, and real-time data collection, monitoring and processing are achieved; relevant management departments remotely monitor the production information and operation conditions of the aflatoxin degradation machines and the oil pressing devices of all the small oil workshops in real time through the management end, when the production information is abnormal or the operation conditions are abnormal, remote control is achieved for oil sampling, and the system ensures accurate management and safety of small oil workshops oil sampling.