32 results about "Pseudomonas aeruginosa DNA" patented technology

The invention discloses a Pseudomonas aeruginosa nucleic acid fluorescent PCR (polymerase chain reaction) detection kit and detection method. The detection kit comprises a PCR reaction solution, an enzyme mixed solution, a positive control, a negative control and an internal standard, wherein the PCR reaction solution comprises a PCR buffer solution, nucleic acid releaser, deoxyribonucleoside triphosphate, primers for target polynucleotide amplification and probes for target polynucleotide detection; the primers comprise a forward primer and a reverse primer. Or, the detection kit comprises a PCR reaction solution, an enzyme mixed solution and an internal standard, wherein the PCR reaction solution comprises a PCR buffer solution, nucleic acid releaser, deoxyribonucleoside triphosphate, a forward primer for target polynucleotide amplification, a reverse primer for target polynucleotide amplification and probes for target polynucleotide detection. The Pseudomonas aeruginosa nucleic acid fluorescent PCR detection kit shown in the embodiment of the invention with the advantages of no need of nucleic acid extraction, high detection sensitivity, wide detection range and high detection accuracy can quickly and accurately detect Pseudomonas aeruginosa DNA (deoxyribonucleic acid) in plasma, urine and other samples.

The invention discloses multiple PCR detection primers of escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, a kit and a detection method. Specific primers are designed by means of combined utilization of escherichia coli alkaline phosphatase gene, pseudomonas aeruginosa outer membrance proteins gene and staphylococcus aureus heat-resistant nuclease gene sequences, multiple PCR detection is conducted on a sample by means of the specific primers according to the method, and specific detection of the escherichia coli, the pseudomonas aeruginosa and the staphylococcus aureus in the sample can be achieved at a time; the detection time is short, operation is easy and convenient, the detection efficiency can be effectively improved, the requirement of rapid detection is met, the sensitivity is high, the specificity is high, and the multiple PCR detection primers of the escherichia coli, the pseudomonas aeruginosa and the staphylococcus aureus, the kit and the detection method can be applied to microbiological detection work of cosmetics.

The invention relates to the field of microorganisms, and specifically discloses a new application of pseudomonas aeruginosa, namely the new application of the pseudomonas aeruginosa to patulin degradation. According to the invention, it is proved by experiments that the pseudomonas aeruginosa can degrade the patulin effectively. The pseudomonas aeruginosa has a good application prospect in developing both of new biodegradation microbial preparations and new biodegradation sterile preparations.

The invention belongs to the field of bioengineering and provides a phage with environment sterilization capacity and application thereof. A phage monomer is the pseudomonas aeruginosa phage, the Latin name is P.aeruginosaphage, the phage monomer is named as Lp14 and has broad-spectrum sterilization capacity on pseudomonas aeruginosa, the phage Lp14 is preserved in the general microbiological center of the China microbe preservation management committee, the preservation date is thirtieth, March, 2017, and the preservation number is CGMCC No.13791. The phage has a high splitting function on pseudomonas aeruginosa, and a phage source is provided for industrial phage production, disinfection and sterilization; the phage can also be used as a safe biological disinfector and used for sterilizing the environment of a mink field, and accordingly the hemorrhagic pneumonia caused by pseudomonas aeruginosa during mink breeding is reduced. The phage can be applied to industrial production, subjected to specific amplification through host pseudomonas aeruginosa, used as the biological disinfector and applied to sterilization for the environment of the mink field.

The present invention relates generally to identification of Pseudomonas aeruginosa bacteria or strains of Pseudomonas aeruginosa, and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis.

The invention discloses a set of primers for detecting Pseudomonas aeruginosa by a loop-mediated isothermal amplification technique and a rapid diagnosis kit for Pseudomonas aeruginosa gene. Prior art Chinese patent CN 102134601 A discloses a loop-mediated isothermal amplification detection primer, detection method and detection kit for Pseudomonas aeruginosa. This invention designs and screens the ecfX gene of Pseudomonas aeruginosa A set of specific detection primers, a detection kit containing the detection primers and the detection of LAMP by using the detection kit. Compared with the prior art, the present invention designs a group of new primers based on the ecfX gene. Through the comparison experiment of two groups of primers, the primers of the present invention have better sensitivity and high sensitivity.

The invention discloses a method for preparing a proteus mirabilis-staphylococcus aureus-pseudomonas aeruginosa adsorption combined vaccine. The method includes the steps that firstly, a proteus mirabilis culture solution is subjected to in-situ digestion, high-speed centrifugation, pre-filtering, ultrafiltration and precipitation, and a proper proteus mirabilis cell membrane soluble antigen raw solution is obtained; secondly, a staphylococcus aureus bacterium suspension is subjected to centrifugation, smashing, re-centrifugation, filtering, precipitation, enzymolysis and dialysis, and a proper staphylococcus aureus cytoplast antigen raw solution is obtained; thirdly, a staphylococcus aureus and pseudomonas aeruginosa culture solution is subjected to centrifugation and pre-filtering, formalin is added for detoxification, then purification is conducted, and a proper inactivated staphylococcus aureus and pseudomonas aeruginosa toxin raw solution is obtained. The vaccine is used for preventing burn, scalding and infection caused by one or more of conditioned pathogens including proteus mirabilis, staphylococcus aureus and pseudomonas aeruginosa before and after operations in an intramuscular deep injection mode.

Provided is a novel antibody having an excellent antibacterial activity against P. aeruginosa. By using plasmablasts obtained from cystic fibrosis patients with chronic P. aeruginosa pulmonary infection as starting materials, antibodies which bind to LPS of a P. aeruginosa strain of serotype E and which have excellent antibacterial activities in vitro and in vivo were successfully obtained.

The invention discloses a pseudomonas aeruginosa OprF-VP22 gene, the application of the gene for making vaccines for controlling pseudomonas aeruginosa infection, and a pseudomonas aeruginosa DNA vaccine containing the gene.

The invention discloses pseudomonas aeruginosa and particularly discloses pseudomonas aeruginosa Gx preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC NO. M2016790 on December 30th 2016. The invention also discloses applications of the pseudomonas aeruginosa Gx to asphalt degradation and transformation, colloid degradation and transformation and microbial enhanced oil recovery. The pseudomonas aeruginosa Gx disclosed by the invention has a strong degradation ability on colloid and unknown macromolecule components in asphalt and crude oil, strong surface active material synthesizing ability and acid producing ability and large oil displacing potential in the microbial enhanced oil recovery of high-asphalt content heavy oil reservoirs; and therefore, the pseudomonas aeruginosa Gx is capable of remarkably reducing the adhesion of the crude oil and increasing the gasoline and diesel oil yield in crude oil refining.

The invention relates to the field of disinfection and sterilization, which discloses a method for killing pseudomonas aeruginosa. The method is achieved only by putting a carrier infected with the pseudomonas aeruginosa in plasma environment at a low temperature of between 10 DEG C and 50 DEG C for killing the pseudomonas aeruginosa for 30-100 seconds, wherein the low-temperature plasma environment is generated by a radio frequency plasma discharge device.

The invention relates to the field of biology and particularly relates to an application for suppressing expression of a gene rsaL in pseudomonas aeruginosa and the pseudomonas aeruginosa with suppressed expression of the gene rsaL. The application is improvement of the transcriptional levels of rhamnolipid-synthesizing genes rhlA and rhlB, the rhamnolipid-synthesizing capability and the swarmingmoving capability of the pseudomonas aeruginosa. According to the transcriptional levels of the rhamnolipid-synthesizing genes rhlA and rhlB and synthesis of total rhamnolipid, dirhamnolipid Rha-Rha-C10-C10 and monorhamnolipid Rha-C10-C10 of the strain provided by the invention, the obtained yield is higher, the property is more stable and the problem of mutation restoration in the process of physicochemical mutagenesis is solved. In addition, the swarming moving capability of the rhamnolipid strain provided by the invention is enhanced. Simultaneously, the strain constructed by the inventionhas the beneficial effects that antibiotics do not need to be added in the production process of rhamnolipid, so that the benefit for the following separation purification is achieved.

An object is to provide an antibody and a vaccine composition which have an ability to practically prevent or treat a Pseudomonas aeruginosa infection, and which can cope with the diversity of clinical isolates derived from patients infected with Pseudomonas aeruginosa. According to the present invention, an antibody against a PA1698 protein that is a type III secretion system component protein of Pseudomonas aeruginosa or against a peptide of the protein, and a vaccine composition comprising the protein or the peptide are provided.

The invention relates to functions of genes of pseudomonas aeruginosa and an application of the genes, wherein the gene BN889_05221, the gene PA0243, the gene PA3808, the gene PA1993 and the gene PA1115 of pseudomonas aeruginosa are the necessary host genes of phage infection, and are used for screening the relevant medicines for inhibiting the phage infection. According to the invention, through the study on the relevant host genes of phage infection, a molecular mechanism for the interaction between phage and host bacteria can be well understood, therefore, a theoretical basis is provided for the treatment on the phage, meanwhile, new target genes related to virus infection can be found, and assistance is provided for screening antiviral drugs.

The invention discloses pseudomonas aeruginosa and a pseudomonas aeruginosa-containing marine mammal vaccine. A used strain is a pseudomonas aeruginosa DCP1 strain separated from a marine mammal, andhas a preservation number of CGMCC NO. 15418. The invention further discloses the marine mammal vaccine. An inactivated pseudomonas aeruginosa antigen of the marine mammal is used as an active component of the marine mammal vaccine. The invention further discloses a preparation method of the marine mammal vaccine. The preparation method comprises processes of separation of the strain, authentication, purification, antigen preparation, vaccine preparation and the like. The pseudomonas aeruginosa inactivated vaccine prepared through the preparation method disclosed by the invention is safe and reliable, can obviously increase the antibody level and has a wide application prospect.

The invention discloses a primer, a kit and a method for detecting pseudomonas aeruginosa and plcH in water, and aims to overcome the shortcomings of the prior art. The sequence of the primer and a probe sequence include an upstream primer P.AF, a downstream primer P.AR, a probe P.AP, an upstream primer plcHF, a downstream primer plcHR and a probe plcHP. The kit is reasonable in composition and proportion, convenient to use, rapid and accurate in detection and applicable to double ddPCR detection of the pseudomonas aeruginosa and plcH genes in the water. The method is used for detecting the pseudomonas aeruginosa and the plcH genes in the water by the kit, simple, convenient and rapid in detection and accurate in detection result.

The present invention discloses a strain of Pseudomonas aeruginosa ZC6, which provides a heavy metal ion Zn<2+> removing effect. The method for screening Pseudomonas aeruginosa providing a heavy metal ion Zn<2+> removing effect from soil and water comprises: a, preparing a metal selective culture medium; b, carrying out enrichment acclimatization on a heavy metal resistance strain; c, screening and re-screening the heavy metal resistance strain; and d, detecting the heavy metal removing capability of the strain. According to the present invention, with the detection test on the heavy metal removing capability of the strain obtained by carrying out enrichment acclimatization on the heavy metal resistance strain with the metal ion-containing culture medium and purification, the reasonable and effective screening method for the heavy metal removing strain is established so as to obtain the strain suitable for requirements; and with application of the Pseudomonas aeruginosa ZC6 in the heavy metal pollution treatment, advantages of no secondary pollution, high treatment efficiency, wide application range, low cost and the like are provided.

The invention discloses a pseudomanas aeruginosa. The pseudomonas aeruginosa is named as pseudomonas aeruginosa DSP-05 preserved in the general microorganism center of China Committee for Culture Collection of Microorganisms with the preservation number of CGMCC No.8390 and the preservation date of October 24, 2013. According to the invention, the pseudomanas aeruginosa is mainly applied to degradation of chlorpyrifos in wastewater, and suitable for popularization and utilization in treating the wastewater that contains chlorpyrifos; immobilized glomerular chlorpyrifos prepared by adopting bacterial strains has the advantages of better effect, low production cost, convenience for utilization and good removal effect; the pseudomonas aeruginosa plays an important role in protecting ecological environment and human health, and reducing the wastewater treatment cost; the pseudomonas aeruginosa DSP-05 is simple in culture condition and easy to keep and for industrial production, and has a good development and application prospect.

The invention discloses a method for preparing a pseudomonas aeruginosa fermentation extract and application thereof in a printing and dyeing fixing agent. The preparation method comprises the following steps: performing constant-temperature water bath treatment on a pseudomonas aeruginosa fermentation solution, cooling, regulating to acidity, and stirring; filtering with plate-frame, performing flash drying on filtrate to obtain a solid fermentation solution; extracting the solid fermentation solution with isopropyl alcohol; transferring the extract into a separating funnel, adding ethyl acetate into the funnel, sufficiently oscillating, and standing; performing rotary evaporation and concentration on supernate, and performing two-stage countercurrent extraction and spray drying on the rotary evaporated concentrate with a centrifuge to obtain the pseudomonas aeruginosa fermentation extract. The pseudomonas aeruginosa fermentation extract can be used for effectively improving the colorfastness index of textiles by applying to a printing and dyeing fixing agent, and does not contain formaldehyde and other harmful ingredients to improve the use performance of the printing and dyeingfixing agent.