Verdigris pseudomonas aeruginosa strain denitrified under different dissolved oxygen conditions and application thereof

A technology based on Pseudomonas aeruginosa and dissolved oxygen, applied in the field of biological denitrification, can solve problems such as low nitrification efficiency, increased power costs, and slow proliferation, and achieve high application value, cost savings, and simplified process flow

Inactive Publication Date: 2010-11-10
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification process eliminates nitrogen pollution to a certain extent, but there are still many problems: (1) every 1g of NH oxidized 4 + -N will consume 7.14g of alkalinity (as CaCO 3 (2) The oxygen equivalent of ammonia nitrogen is 4.57g, which requires sufficient aeration during the nitrification process, which increases the power cost; (3) The denitrification process Additional carbon sources are required, which increases operating costs; (4) Nitrifying bacteria are usually autotrophic bacteria, with slow proliferation and low nitrification efficiency; (5) Sludge backflow and nitrifying liquid backflow increase the power and operating costs of the system
However, there are few reports on simultaneous nitrification and denitrification under anoxic conditions.

Method used

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  • Verdigris pseudomonas aeruginosa strain denitrified under different dissolved oxygen conditions and application thereof
  • Verdigris pseudomonas aeruginosa strain denitrified under different dissolved oxygen conditions and application thereof
  • Verdigris pseudomonas aeruginosa strain denitrified under different dissolved oxygen conditions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. Identification of strain HD4-2

[0066] 1. Individual morphological characteristics of the strain: negative Gram stain; rod-shaped; no spores and capsules.

[0067] 2. Bacterial colony morphological characteristics: bacterial strain is on plate (every L culture medium contains 5g sodium succinate, 0.543g K 2 HPO 4 ·3H 2 O, 0.5gNaNO 3 , 0.1g KCl, 0.1g MgSO 4 ·7H 2 O, 0.0005g FeSO 4 ·7H 2 (0, pH is 7.0, add 1.5%-2.0% agar) after cultivating on 2 days, form the circular colony of 2-3mm, white, flat, smooth surface, produce yellow-green pigment.

[0068] 3. Characteristics of 16S rRNA gene sequence: its 16S rRNA has the nucleotide sequence shown in the sequence table, and the sequence length is 1055bp.

[0069] According to its morphological characteristics and 16S rRNA gene sequence characteristics, strain HD4-2 was identified as Pseudomonas aeruginosa.

Embodiment 2

[0070] Example 2. Biological denitrification experiment under anaerobic conditions

[0071] Anaerobic conditions refer to filling 100mL of culture medium in a 100mL anaerobic bottle, closing the lid, and then sterilizing at 121°C for 30 minutes; after inoculating the bacterial suspension under sterile conditions, seal the anaerobic bottle; first use a vacuum pump to evacuate , and then filled with nitrogen, repeating this operation 3 times to ensure that anaerobic conditions are achieved; finally, place the anaerobic bottle at 30° C., shake the culture in a shaker at 50 rpm (so that the bacteria and the culture solution are fully mixed).

[0072] The strain HD4-2 was inoculated in a 250mL Erlenmeyer flask containing 100mL medium (each L medium contained 5g sodium succinate, 0.543g K 2 HPO 4 ·3H 2 O, 0.5g NaNO 3 , 0.1g KCl, 0.1g MgSO 4 ·7H 2 O, 0.0005g FeSO 4 ·7H 2 O, pH is 7.0), 30 ℃, 150rpm shaker shake culture 20-24h. When OD 600 When it is 0.9-1, the culture is sto...

Embodiment 3

[0076] Example 3. Biological denitrification experiment under anoxic conditions

[0077] The anoxic condition refers to the situation in which the culture medium of the inoculated bacterial suspension is statically placed in a biochemical incubator.

[0078] Please note: hypoxic conditions and the following microaerobic conditions and aerobic conditions are common knowledge known to those skilled in the art, so they will not be described in detail here.

[0079] 1. Biological denitrification experiment using ammonia nitrogen as nitrogen source

[0080] Bacterial strain HD4-2 was inoculated in 500mL Erlenmeyer flask containing 300mL medium (every L medium contains 5g sodium succinate, 0.543g K 2 HPO 4 ·3H 2 O, 0.3147g NH 4 Cl, 0.1g KCl, 0.1g MgSO 4 ·7H 2 O, 0.0005g FeSO 4 ·7H 2 O, pH is 7.0), 30 ℃, 150rpm shaker shake culture 20-24h. When OD 600 When it is 0.9-1, the culture is stopped, and the bacterial suspension is used for inoculation.

[0081] Get 10mL prepared ...

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Abstract

The invention discloses a verdigris pseudomonas aeruginosa strain denitrified under different dissolved oxygen conditions and the application thereof and also discloses a microbiological bacterial inoculum for biological denitrification. The pseudomonas aeruginosa HD4-2 provided by the invention was preserved in CGMCC (China General Microbiological Collection Center) on Jan. 21 in 2010 and has a preservation number of CGMCC No.3602. The strain can remove ammonia nitrogen under anoxic, micro-aerobic and aerobic conditions with the removal rate over 95%, can independently finish the whole process of biological denitrification without the accumulation of nitrite nitrogen and nitric nitrogen and has higher potential application values in practice.

Description

technical field [0001] The invention relates to the field of biological denitrification, in particular to Pseudomonas aeruginosa strains for denitrification under different dissolved oxygen conditions and applications thereof, and also to a microbial bacterial agent for biological denitrification. Background technique [0002] Nitrogen in water includes inorganic nitrogen and organic nitrogen. Inorganic nitrogen includes ammonia nitrogen, nitrite nitrogen and nitrate nitrogen; organic nitrogen includes urea, amino acid, protein, nucleic acid, uric acid, organic base, amino sugar and other nitrogen-containing organic compounds. Nitrogen pollution in water will not only lead to eutrophication of water, but also endanger the survival of organisms and human beings. [0003] Compared with physical and chemical nitrogen removal technology, biological nitrogen removal technology has low cost and no secondary pollution, so it has been widely used in nitrogen removal. The theoretic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/385C02F101/16C02F101/38
Inventor 倪晋仁仝国平陈倩
Owner PEKING UNIV
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