234 results about "Nitrite nitrogen" patented technology

The invention relates to the technical field of microorganisms, and discloses a sewage aquatic microbe with ammoxidation capacity, a microbial agent and application of the microbial agent. The strain name of the sewage aquatic microbe is B1, and the preservation number of the sewage aquatic microbe is CCTCC No: M 2025640. The sewage aquatic microbacterium B1 provided by the invention can convert ammonia nitrogen into nitrite nitrogen, so that short-cut nitrification is realized in wastewater treatment, and the strain can tolerate and effectively treat ammonia nitrogen with relatively high concentration, and has relatively good adaptive capacity to environments with different pH values, salinity and temperatures.

The invention discloses a high-activity short-cut nitrification quick-start long-term stable operation process, which relates to the technical field of biological sewage treatment, and comprises the following steps: inoculating sludge, carrying out anaerobic fermentation pretreatment, and removing expanded sludge and sludge foam in combination with quick sludge discharge and manual removal; controlling the number of days of sludge age, reproliferating the sludge, enriching ammonia oxidizing bacteria, and inhibiting and elutriating nitrite oxidizing bacteria; long-term operation is carried out under the condition of no sludge discharge, the abundance and activity of ammonia oxidizing bacteria are maintained, and meanwhile, the abundance of nitrite oxidizing bacteria is controlled; the proliferation of nitrite oxidizing bacteria is controlled by using the predation effect of protozoa and metazoan, and the accumulation rate of nitrite in the reactor is controlled; monitoring the dissolved oxygen, the pH value and the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the reactor in real time, and regulating and controlling the influent ammonia nitrogen load and the free ammonia concentration; dissolved oxygen distribution is adjusted in an intermittent aeration or microporous aeration mode, and the operation conditions of the short-cut nitrification reactor are optimized. The method is simple to operate, economical and effective.

The invention belongs to the technical field of sewage treatment, and particularly relates to an anaerobic ammonia oxidation-photoelectric denitrification deep nitrogen removal method. The method comprises the following steps: firstly, adopting a double-chamber reactor; introducing part of sewage into the cathode chamber, carrying out Anammox reaction under a dark state condition to remove ammonia nitrogen and part of nitrite nitrogen, and synchronously generating nitrate nitrogen; turning on a light source to irradiate the anode chamber, exciting photo-induced electrons by a photosensitizer, transmitting the photo-induced electrons to the cathode chamber through an external circuit, and driving the sulfur autotrophic denitrification sludge to reduce residual nitrite nitrogen and nitrate nitrogen generated by Anammox; the total nitrogen removal rate is close to 100%. According to the invention, photo-induced electrons are used as the only denitrification electron donor, so that the addition of a carbon source / sulfide is thoroughly avoided, and the risk of secondary pollution is eliminated. The method is suitable for green low-carbon deep treatment of sludge digestion liquid and pharmaceutical wastewater high-ammonia-nitrogen low-carbon-nitrogen-ratio sewage.

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses a nitrite concentration monitoring method based on a sensor coupling soft measurement model. The method comprises the following steps: S1, acquiring target influence factor data; determining a plurality of target influence factors based on the water quality parameters and the nitrification process of the to-be-monitored sewage; s2, establishing a soft measurement model; the input of the soft measurement model is the target influence factor, and the output of the soft measurement model is the concentration of nitrite nitrogen; s3, determining the concentration of nitrite nitrogen; according to the method, the target influence factor is firstly acquired by utilizing the sensor, and the nitrite concentration is calculated or predicted in real time by utilizing the target influence factor, so that the problems that monitoring equipment is expensive, the monitoring range is small, the monitoring is inaccurate and the monitoring equipment cannot directly monitor the nitrite in the seriously polluted wastewater in the nitrite monitoring in the prior art can be solved.

The invention discloses a virulence factor gene relative abundance prediction model based on water quality physicochemical indexes, and relates to the technical field of ecological environment protection, and the virulence factor gene relative abundance prediction model comprises the following steps: taking a plurality of river water samples, obtaining virulence factor gene relative abundance of the river water samples, and obtaining water quality physicochemical index data of the river water samples; temperature, redox potential, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, total nitrogen, total dissolved solids, pH, conductivity, chlorophyll-a, chemical oxygen demand, dissolved oxygen, total organic carbon, total phosphorus, organic nitrogen, phosphate, carbon-nitrogen ratio and organic nitrogen / total nitrogen are taken as explanatory variables, and relative abundance of virulence factor genes is taken as a target variable. And establishing a regression model by adopting a GBM algorithm in an h2o packet in R software, and predicting the relative abundance of the virulence factor gene of the river water sample to be detected by utilizing the prediction model. The research provides a brand new method for predicting the relative abundance of the virulence factor gene and analyzing the environmental response of the virulence factor gene, and the method is suitable for identification and interpretation of a high-dimensional nonlinear ecosystem with complex interaction.

The present invention discloses a strain of Stutzerimonas kunmingensis and its application in water denitrification, relating to the field of biotechnology. The Stutzerimonas kunmingensis was deposited in the General Microbiology Center (CGMCC) of the China Culture Collection of Microorganisms on April 24, 2024, with a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and a deposit number of CGMCCNO.30427. The present invention isolated and obtained Stutzerimonas kunmingensis 9‑4 from a water sample of a crucian carp recirculating aquaculture system. The strain has a good removal effect on nitrate nitrogen, nitrite nitrogen and ammonia nitrogen in the water environment. At the same time, adding hematite under light conditions can significantly improve the removal rate of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen by the Stutzerimonas kunmingensis, which can be applied to the removal of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen in aquaculture water environment and aquaculture tail water.

The invention relates to the technical field of sewage treatment, in particular to an internal and external backflow automatic control system for sewage treatment. The system comprises an internal reflux system and an external reflux system, and the internal reflux system adjusts the frequency of internal reflux pumps or the number of started internal reflux pumps by monitoring the change of water quality parameters such as nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and dissolved oxygen so as to maintain the balance of nitrification and denitrification reactions; and the external reflux system adjusts the flow of an external reflux pump by monitoring the sludge concentration and the sludge level height so as to maintain the concentration and activity of microorganisms in the biological reaction tank. Besides, the system also adopts a mathematical model, a feedforward control strategy and a feedback control module, so that the response speed and the stability of the system are improved, the effective treatment of sewage is ensured, and the overall treatment efficiency and the effluent quality are improved.

The application discloses a method for efficient denitrification and decarburization of kitchen waste and sludge co-fermentation anaerobic digestion effluent, which removes part of COD and ammonia nitrogen in the influent sludge by using a first SNAD, and maintains a certain amount of free ammonia of a certain concentration due to the high concentration of ammonia nitrogen and the high pH of the influent, so as to maintain the inhibition effect on nitrifying bacteria and ensure the conversion of part of the ammonia nitrogen into nitrite nitrogen, and the high activity of anaerobic ammonia oxidation bacteria is ensured in the first SNAD by limiting the aeration amount, so that the ammonia nitrogen and nitrite can be further removed; the effluent enters a nitrification tank, and the ammonia nitrogen is nitrified into nitrate nitrogen under the condition of nitrifying bacteria and sufficient aeration amount, and then enters a denitrification tank, and the denitrification process is carried out under the condition of heterotrophic denitrifying bacteria and sufficient carbon source, so that most of the nitrate nitrogen in the system is converted into nitrogen gas and removed, finally, the clear liquid is discharged after being filtered by an MBR membrane in a membrane tank, so as to achieve the purpose of efficient denitrification and decarburization of kitchen waste anaerobic digestion effluent.

A method for in-situ efficient enrichment of anaerobic ammonia oxidation bacteria, comprising: using anaerobic digestion sludge as inoculation sludge and using nitrate wastewater as an influent substrate, the inoculation sludge and the influent substrate reacting with each other under a light-proof, constant-temperature and closed condition so as to enrich anaerobic ammonia oxidation bacteria. After the anaerobic ammonia oxidation bacteria are enriched about 30 days by using the anaerobic digestion sludge as the inoculation sludge and the nitrate wastewater as the influent substrate, the number of functional genes HzsB of the anaerobic ammonia oxidation bacteria is increased by about 100 times than that in a control group (using a liquid mixture of ammonia nitrogen and nitrite nitrogen as an influent substrate). Thus, on one hand, there is no need to add extra ammonia nitrogen and nitrite nitrogen, and on the other hand, there is no need to regulate and control complex process parameters. Therefore, in-situ rapid and sustainable enrichment of anaerobic ammonia oxidation bacteria can be implemented in wastewater treatment plants simply by means of an economical and simple operation mode.

The invention relates to the technical field of water treatment, and discloses a sludge quality-divided reflux type aerobic-anoxic sulfur autotrophic activated sludge denitrification process. The process comprises the following steps: wastewater enters a sectional type aerobic tank, the wastewater enters the sectional type aerobic tank, organic matters are removed in a strengthened manner in a first section of aerobic tank, part of ammonia nitrogen is converted into nitrite nitrogen and nitrate nitrogen, and nitrification is strengthened in a second section of aerobic tank, so that the ammonia nitrogen is deeply removed; effluent of the sectional type aerobic tank enters an anoxic tank and is completely mixed with sulfur particles and activated sludge for sulfur autotrophic denitrification; effluent of the anoxic tank enters a secondary sedimentation tank for sedimentation, supernate is discharged, and bottom mud enters a hydrocyclone to be divided into heavy sludge and light sludge; the heavy sludge flows back to the front end of the anoxic tank, the light sludge flows back to the front end of the sectional aerobic tank, and the remaining sludge is discharged. According to the invention, sulfur autotrophic denitrification is deeply integrated into an activated sludge system, so that high-efficiency nitrogen removal without carbon source and internal reflux is realized.

The invention relates to the field of fish and vegetable comprehensive cultivation, in particular to a photovoltaic fish and vegetable symbiotic system which comprises a photovoltaic system, a fishery breeding system and a circulating purification system, a fishpond is arranged below a photovoltaic panel, net cages are arranged in the fishpond for fish breeding, the circulating purification system is arranged around the fishpond, and water is pumped in the fishpond through a pump station; water quality purification and water quality maintenance are carried out on the pumped water through a precipitation filtration system, a microfilter, a hydroponic vegetable system and an ultraviolet disinfection system in sequence, so that the water body in the pond is ensured to fully and circularly flow, and the concentration of chemical substances such as ammonia nitrogen, nitrite nitrogen and the like which are harmful to fishes is reduced. Through the synergistic effect of all parts of the photovoltaic fish and vegetable symbiotic system, the problem that the water quality in the aquaculture environment under the photovoltaic panel is prone to deterioration is effectively solved, and the aquaculture benefits are improved.

The invention discloses a water quality parameter measurement method driven by a multilayer deep learning model, which realizes high-precision measurement of key water quality parameters such as COD (Chemical Oxygen Demand), total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), nitrite nitrogen (NO3-N), ammonia nitrogen (NH3-N), turbidity and chromaticity by fusing CNN (convolutional kernel 3 * 1 and kernel number 32 / 64), BiLSTM (hidden nodes 30-60) and Attention mechanism and combining WOA hyper-parameter optimization (learning rate 0.001-0.01). According to the method, Savitzky-Golay filtering (window 11, order 3) denoising is adopted, data quality is enhanced through normalization preprocessing, model robustness is improved through 10-fold cross validation and an Adam optimizer, the problem that a traditional model is poor in adaptability to complex spectrum time sequence data is solved, and the method is suitable for rapid online monitoring of water quality.

The invention discloses a device and a method for cooperatively treating domestic sewage and nitrate wastewater by coupling heterotrophic denitrification and sulfur autotrophic denitrification with anaerobic ammonia oxidation, and belongs to the field of biological denitrification of sewage. The device comprises a water inlet tank, a sequencing batch reactor (SBR), a peristaltic pump, a drain valve, a water outlet tank and the like. The method comprises the following steps: (1) adding sulfur particles into a first SBR (Sequencing Batch Reactor), introducing nitrate wastewater and domestic sewage into the SBR, converting nitrate nitrogen into nitrogen through sulfur autotrophic denitrification under an anaerobic condition, and reducing the nitrate nitrogen into nitrite nitrogen when COD (Chemical Oxygen Demand) comes from the domestic sewage; and (2) adjusting the proportion of ammonia nitrogen and nitrite in the effluent of the first SBR, and stably removing ammonia nitrogen and nitrite through anaerobic ammonia oxidation. In the two-stage system, 100% of aeration and external carbon sources are saved, and a new thought is provided for synergistic denitrification of pure autotrophic systems of industrial wastewater and domestic sewage.

The present invention discloses a rapid total nitrogen detection method for early warning, specifically related to the technical field of water quality monitoring. This method first detects and records multiple groups of data on the ammonia nitrogen value, nitrate nitrogen value, nitrite nitrogen value, pH value, and total nitrogen value at different positions in the water quality. Then, a linear model is established by summing the ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen values and the total nitrogen value. Next, an accurate algorithm model is obtained by inversely compensating the total nitrogen value based on the pH value. Subsequently, the model is input into the internal system of the total nitrogen sensor connected to the early warning analysis unit. Finally, the sensor is installed in the water area to be detected for rapid detection and early warning. Its advantages are rapid detection, accurate results, and small sensor volume for easy installation, effectively solving many drawbacks of traditional total nitrogen detection methods, and can be widely applied to the water quality monitoring of various water areas, providing early warning for water eutrophication and other situations in a timely manner.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

The invention discloses a domestic sewage treatment system and method based on carbon capture and autotrophic nitrogen removal. The domestic sewage treatment system mainly comprises a carbon capture reactor, a nitrification reactor and a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation reactor. Carbon and phosphorus in the sewage are removed in the carbon capture reactor through flocculent precipitation and are used for energy and resource recovery; nitrogen in effluent of the carbon capture reactor is removed through an autotrophic nitrogen removal technology; a part of sewage enters a nitration reactor for complete nitration; nitration effluent containing nitrate nitrogen and carbon capture reactor effluent containing ammonia nitrogen are mixed and then pumped into a sulfur autotrophic denitrification coupling anaerobic ammonia oxidation reactor; in the sulfur autotrophic denitrification coupling anaerobic ammonia oxidation unit, nitrate nitrogen is converted into nitrite nitrogen by sulfur autotrophic denitrification, and anaerobic ammonia oxidation realizes autotrophic nitrogen removal by using ammonia nitrogen and nitrite nitrogen. According to the invention, carbon and nitrogen in sewage are separated, carbon in wastewater is effectively captured and utilized, and nitrogen in sewage is effectively removed through a low-energy-consumption autotrophic nitrogen removal system.

The invention provides a bacterial strain YB070 (Acinetobacter indicus YB070), and the bacterial strain is separated from activated sludge of a nitrogen-containing wastewater treatment system. The YB070 strain can grow under anaerobic, aerobic and anoxic conditions, the growth under the aerobic condition is the best, and the growth under the anoxic condition is the second. The strain can grow well in a pH range of 5-9, and is preferred to an acidic environment. And when the NaCl concentration is greater than 7%, the growth is inhibited. YB070 can tolerate high-concentration ammonia nitrogen and can effectively remove ammonia nitrogen under the aerobic condition, and the removal rate of 367mg / L ammonia nitrogen can reach 34.1%. Nitrate nitrogen and nitrite nitrogen in a water body can be removed under the aerobic condition and the anaerobic condition. The removal rates of nitrate nitrogen and nitrite nitrogen respectively reach 92% and 100%. The strain has a good application prospect in sewage denitrification.

The invention provides an efficient resourceful treatment method for industrial wastewater, and belongs to the technical field of wastewater treatment. The method comprises the following steps: adjusting the pH value of wastewater to be treated to 6.8-7.4, adding a composite reducing agent, reacting to obtain a reaction solution, and filtering and separating the reaction solution to obtain a nitrogen-containing filtrate and filter residues; mixing the nitrogen-containing filtrate, magnesium hydroxide and phosphoric acid, adjusting the pH value to 8.5-9.2, reacting, and centrifuging to obtain a supernatant and a solid matter; and performing post-treatment on the supernate to obtain effluent, and drying the solid matter to obtain the nitrogen fertilizer. According to the method, nitrate nitrogen and nitrite nitrogen in the wastewater are reduced into ammoniacal nitrogen through the composite reducing agent, and then the ammoniacal nitrogen is solidified and crystallized through the nitrogen fixing agent. According to the method, inorganic nitrogen in wastewater is used as a raw material, conversion from nitrate nitrogen to ammoniacal nitrogen is completed by means of a composite reducing agent, efficient fixation of ammoniacal nitrogen is achieved, the situation that nitrogen escapes in the form of nitrogen is avoided, the nitrogen is converted into solid nitrogen fertilizer, and available resources are provided for downstream related enterprises.

The invention relates to the technical field of water treatment, in particular to a short-cut nitrification process which comprises a water inlet area, a short-cut nitrification area and the like, nitrate nitrogen is refluxed by a circulating pump and sprayed to a filler layer through a water distribution pipe to realize denitrification, flora mass transfer and biological membrane updating are promoted by combining mechanical shear force, and a DO gradient is formed through aeration to realize coupling of synchronous nitrification and short-cut nitrification. And ammonia nitrogen and nitrite nitrogen directly generate nitrogen. According to the process, the denitrification efficiency can be improved to 95% or above, the aeration energy consumption is reduced by 40-50%, the sludge yield is reduced by 30% or above, and the process is low in equipment investment, high in adaptability and suitable for high-ammonia-nitrogen industrial wastewater treatment.

The invention relates to the technical field of sewage treatment, in particular to a method for rapidly measuring BOD (Biochemical Oxygen Demand) in sewage treatment. According to the method, under the anaerobic condition, facultative anaerobic microorganisms are subjected to denitrification reaction by utilizing organic matters and nitrate nitrogen in a water sample to be detected, in the process, the organic matters are oxidized into carbon dioxide and water by utilizing atomic oxygen in the nitrate nitrogen, and the nitrate nitrogen is reduced into nitrite nitrogen or nitrogen; in the process, the reduction amount of the oxygen element in the reaction system before and after the reaction is the BOD of the water sample to be detected. Compared with traditional BOD5, the method disclosed by the invention is shorter in time consumption and greatly improves the detection efficiency, and meanwhile, compared with an aerobic method during BOD5 measurement, the method disclosed by the invention effectively avoids the oxygen consumption of aerobic proliferation of microorganisms in the process, so that the detection accuracy is improved, and the method is convenient to popularize and use.

This invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and its applications. The heterotrophic-aerobic denitrifying bacterium C-3 is deposited at the China General Microbiological Culture Collection Center (CGMCC), classified as *Brevundimonas naejangsanensis*, with accession number CGMCC No. 31729, and deposited on August 23, 2024. The heterotrophic-aerobic denitrifying vesicular *Brevundimonas naejangsanensis* strain C-3 provided by this invention exhibits excellent nitrate nitrogen removal efficiency (100%) under aerobic conditions using sodium acetate as a carbon source, a C / N mass ratio of 10, a pH of 7, a temperature of 30℃, and a rotation speed of 200 r / min. In actual aquaculture wastewater, it demonstrates excellent removal effects on nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, and total nitrogen. Furthermore, this strain exhibits high safety and has significant application value in treating aquaculture wastewater.

1. The name of the design product: full-spectrum multi-parameter water quality online analyzer (SECCO-L-1905). 2. The use of the design product: for analyzing and detecting key water quality indicators such as total phosphorus, total nitrogen, COD, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, etc. in water, online monitoring, fixed and mobile monitoring of water quality in "source-net-plant-river" multiple scenes, and water pollution prevention and supervision. 3. The design points of the design product: the combination of the shape and pattern of the product as a whole. 4. The picture or photo that best indicates the design points: perspective view 1.

The application relates to a method and device for degrading ethion and recycling heavy metals simultaneously based on an endogenous sulfur cycle, and belongs to the technical field of dressing wastewater treatment. The device is mainly composed of sequentially connected MABR and H2-MBfR. In the method, ethion is oxidized and degraded to produce CS2 and ammonia nitrogen under aerobic conditions in the MABR, and the CS2 is further converted into sulfate; the ammonia nitrogen is nitrified into nitrate nitrogen and nitrite nitrogen. Subsequently, the effluent enters the H2-MBfR, and under anaerobic conditions, the nitrate nitrogen and the nitrite nitrogen are reduced into nitrogen gas by taking hydrogen as an electron donor, and the endogenous sulfate is reduced into sulfur ions; the sulfur ions react with heavy metal ions in situ to generate metal sulfide precipitates. Finally, the precipitates are recovered through solid-liquid separation and are reused for metal flotation. The application realizes directional conversion and collaborative removal of pollutants, does not produce secondary pollution, and realizes recycling of heavy metal resources by using the sulfur element of the pollutants.

The utility model provides an anaerobic ammonia oxidation reaction system. The reaction system comprises a wastewater treatment system, the wastewater treatment system comprises: a first reactor for removing nitrate nitrogen in wastewater and producing first treated water; the second reactor is used for removing ammonia nitrogen and nitrite nitrogen in the first treated water and producing second treated water; the third reactor is used for adjusting the nitrogen composition and concentration of the second treated water and producing third treated water; and the fourth reactor is used for reducing nitrate nitrogen in the third treated water into nitrogen so as to reduce the concentration of total nitrogen in the effluent. The utility model solves the technical problems that an anaerobic ammonia oxidation water treatment system adopting a traditional activated sludge process is insufficient in total nitrogen treatment capacity during operation, and particularly cannot effectively remove nitrate nitrogen in an anaerobic ammonia oxidation reaction product; in the prior art, the treated water can still contain a certain amount of nitrogen pollutants and cannot meet the requirements of discharged water quality.

The invention discloses a rapid nitrite nitrogen enrichment method based on flow regime optimization, a wastewater low-carbon denitrification system and application, according to the method, a microenvironment suitable for AOB growth and nitrite nitrogen accumulation is accurately induced to be formed in a reactor through hydrodynamic regulation and control of an MBBR, so that treated water contains rich nitrite nitrogen, and the content of nitrogen in the wastewater is increased. And the water outlet is further connected with a denitrification biological nitrogen removal process or an anaerobic ammonia oxidation biological nitrogen removal process to form a wastewater low-carbon nitrogen removal system. The coupling system realizes a complete denitrification path under the condition of no additional or low carbon source, is suitable for the treatment requirements of urban sewage and industrial wastewater with low C / N ratio, and has the remarkable engineering advantages of low energy consumption, low carbon source requirement, high operation stability and the like.

The invention discloses Pseudomonas hunanensis N6-2 and application thereof, and belongs to the technical field of environmental microorganisms, the Pseudomonas hunanensis N6-2 is separated from the wetland of the Yellow River Delta, can grow by taking ammonia nitrogen, nitrate nitrogen and nitrite nitrogen as a unique or mixed nitrogen source, and has the functions of heterotrophic nitrification and aerobic denitrification at the same time. The strain has very high treatment efficiency on sewage with the nitrogen concentration of 500 mg / L or below, can tolerate high-salt-alkali sewage with the salinity of 50 g / L and the pH value of 10, has wide environmental adaptability, is non-toxic and harmless in the treatment process and eco-friendly, and has great application potential.

A highly efficient denitrifying strain with the function of dissimilatory reduction to ammonium and its application, which relates to the field of water treatment. The present invention aims to screen out functional strains with the function of efficiently removing nitrate nitrogen or nitrite nitrogen with natural growth advantages. The Aeromonas provided by the present invention has high nitrate and nitrite enzyme activities and rapid cell growth. The strain also has the function of dissimilatory reduction to ammonium, which can achieve the rapid removal of nitrate nitrogen in water and effectively reduce the generation of nitrogen oxide gases. The Aeromonas media L20, which is an aerobic denitrifying bacterium, is preserved in the China Center for Type Culture Collection. The preservation date is July 12, 2023, and the preservation number is CCTCC NO: M 20231191.

The invention discloses a low-temperature denitrification strain and application of the low-temperature denitrification strain. The strain is Acinetobacter Bragg FX1, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 36583. The strain can efficiently remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water at the temperature of 5-30 DEG C, especially shows the optimal denitrification performance at the temperature of about 10 DEG C, and the removal rate of the ammonia nitrogen and the nitrite nitrogen can reach 99% or above. The strain has strong adaptability to low-temperature environment, can tolerate water quality fluctuation of actual sewage, and solves the problems of low denitrification efficiency and unstable operation caused by insufficient microbial activity of sewage treatment plants in low-temperature regions. The strain can be used for preparing a low-temperature sewage denitrification microbial agent, can be directly added into a low-temperature domestic sewage or industrial wastewater treatment system, does not need to depend on high-energy-consumption heating and heat preservation measures, and has remarkable energy-saving and consumption-reducing potential, good environmental adaptability and wide application prospects.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.