281 results about "Nitrite nitrogen" patented technology

The invention discloses a sulfur-manganese composite autotrophic denitrification filler as well as a preparation method and application thereof, and belongs to the technical field of environmental functional materials and polluted water treatment. The sulfur-manganese composite autotrophic denitrification filler contains manganese salt, a sulfur-based component and a cement-based component, the manganese salt comprises one or more of manganese carbonate, calcium manganese ore and rhodochrosite; the sulfur-based component comprises sulfur and sulfur manganese ore; the cement-based component comprises cement, fly ash pottery sand, silica fume, blast furnace slag and metakaolin; the cement comprises one or more of Portland cement, iron aluminate cement and sulphoaluminate cement. The nitrogen removal efficiency of the sulfur-manganese composite autotrophic denitrification filler is superior to that of a sulfur autotrophic denitrification system, the sulfate concentration and nitrite nitrogen accumulation in effluent are remarkably reduced, the service life of the filler is prolonged, and the efficient utilization rate of the material is improved.

A device and method for enhancing nitrogen and phosphorus removal based on a multistage AO partial denitrification coupled with Anammox process in combination with a sludge hydrolytic acidification mixture belong to the technical field of active sludge method wastewater treatment. A system includes a water tank, a water pump, a biochemical reaction zone, a hydrolytic acidification tank and other devices. A multistage AO step-feed pipeline is used to inject raw water into the reaction zone in a segmented manner, guaranteeing efficient utilization of organic matter in the raw water; biofilm carriers are added into an anaerobic zone and anoxic zones to enrich anammox bacteria, and nitrite nitrogen produced by partial denitrification provides a substrate for the anammox bacteria to realize autotrophic nitrogen removal; a nitrification and phosphorus accumulating bacteria aerobic phosphorus uptake are performed in aerobic zones; and part of excess sludge in a secondary sedimentation tank enters the hydrolytic acidification tank to convert macromolecular organic matter into low molecular weight organic matter, a hydrolytic acidification mixture and the excess sludge in the secondary sedimentation tank synchronously flow back to the anaerobic zone, and as a high-quality carbon source, the low molecular weight organic matter can promote partial denitrification. The system provides a novel method for efficient and energy-saving treatment of municipal wastewater.

The invention relates to the technical field of microorganisms, and discloses a sewage aquatic microbe with ammoxidation capacity, a microbial agent and application of the microbial agent. The strain name of the sewage aquatic microbe is B1, and the preservation number of the sewage aquatic microbe is CCTCC No: M 2025640. The sewage aquatic microbacterium B1 provided by the invention can convert ammonia nitrogen into nitrite nitrogen, so that short-cut nitrification is realized in wastewater treatment, and the strain can tolerate and effectively treat ammonia nitrogen with relatively high concentration, and has relatively good adaptive capacity to environments with different pH values, salinity and temperatures.

The invention discloses a high-activity short-cut nitrification quick-start long-term stable operation process, which relates to the technical field of biological sewage treatment, and comprises the following steps: inoculating sludge, carrying out anaerobic fermentation pretreatment, and removing expanded sludge and sludge foam in combination with quick sludge discharge and manual removal; controlling the number of days of sludge age, reproliferating the sludge, enriching ammonia oxidizing bacteria, and inhibiting and elutriating nitrite oxidizing bacteria; long-term operation is carried out under the condition of no sludge discharge, the abundance and activity of ammonia oxidizing bacteria are maintained, and meanwhile, the abundance of nitrite oxidizing bacteria is controlled; the proliferation of nitrite oxidizing bacteria is controlled by using the predation effect of protozoa and metazoan, and the accumulation rate of nitrite in the reactor is controlled; monitoring the dissolved oxygen, the pH value and the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the reactor in real time, and regulating and controlling the influent ammonia nitrogen load and the free ammonia concentration; dissolved oxygen distribution is adjusted in an intermittent aeration or microporous aeration mode, and the operation conditions of the short-cut nitrification reactor are optimized. The method is simple to operate, economical and effective.

The invention belongs to the technical field of sewage treatment, and particularly relates to an anaerobic ammonia oxidation-photoelectric denitrification deep nitrogen removal method. The method comprises the following steps: firstly, adopting a double-chamber reactor; introducing part of sewage into the cathode chamber, carrying out Anammox reaction under a dark state condition to remove ammonia nitrogen and part of nitrite nitrogen, and synchronously generating nitrate nitrogen; turning on a light source to irradiate the anode chamber, exciting photo-induced electrons by a photosensitizer, transmitting the photo-induced electrons to the cathode chamber through an external circuit, and driving the sulfur autotrophic denitrification sludge to reduce residual nitrite nitrogen and nitrate nitrogen generated by Anammox; the total nitrogen removal rate is close to 100%. According to the invention, photo-induced electrons are used as the only denitrification electron donor, so that the addition of a carbon source / sulfide is thoroughly avoided, and the risk of secondary pollution is eliminated. The method is suitable for green low-carbon deep treatment of sludge digestion liquid and pharmaceutical wastewater high-ammonia-nitrogen low-carbon-nitrogen-ratio sewage.

The invention discloses a complex microbial inoculant and application thereof, and belongs to the technical field of microorganisms. The composite microbial agent comprises bacillus (bacillus sp.BS-K1) and pseudomonas sp.CN-B1), and the preservation number of the bacillus is CCTCC (China Center for Type Culture Collection) NO: M 20242022, and the preservation number of the pseudomonas is CCTCC NO: M 20242022. The preservation number of the pseudomonas is CCTCC (China Center for Type Culture Collection) NO: M 20242021. According to the complex microbial inoculant provided by the invention, probiotics (Bacillus sp.BS-K1) and denitrifying bacteria (Pseudomonas sp.CN-B1) which have antibacterial effects are compounded to obtain the complex microbial inoculant for aquaculture, and when the complex microbial inoculant is applied to an aquaculture water body, the complex microbial inoculant has excellent denitrification capability and an inhibition effect on related pathogenic bacteria in the water body; the removal efficiency of ammonia nitrogen and nitrite nitrogen in the culture water body can be improved, the number of related pathogenic bacteria is reduced, and the safety of the culture environment is guaranteed.

The invention discloses a nitrifying bacterium agent suitable for aquarium, fishpond and other aquatic industries, the strain of the nitrifying bacterium agent is Glutamicacter nicotianae TJ-X, the nitrifying bacterium agent is preserved in Guangdong Microbial Culture Collection Center on February 12, 2025, and the preservation number is GDMCC NO.65877. The invention further discloses a preparation method of the nitrifying bacterium agent. The nitrifying bacteria TJ-X strain suitable for the aquarium industry can perform heterotrophic nitrification and aerobic denitrification under the condition that the salt content is 5wt%, under the condition of laboratory pure culture, within 8 hours, the removal rate of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen is 99% or above, the COD degradation rate is 81%, the nitrifying bacteria TJ-X strain is suitable for the environment where organic matter, ammonia nitrogen, nitrite and nitrate coexist, and the nitrifying bacteria TJ-X strain can be applied to the aquarium industry. The microbial agent has the functions of degrading organic matters such as excrement of ornamental fish and fish food, converting organic nitrogen into inorganic nitrogen, and simultaneously degrading inorganic nitrogen such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen into nitrogen. And the strain is high in biological safety, high in environmental adaptability and high in growth density, can be applied to water industries with high organic matter content and inorganic nitrogen, such as the aquarium industry and aquaculture, and has a wide application prospect.

The invention discloses a nitrite concentration monitoring method based on a sensor coupling soft measurement model. The method comprises the following steps: S1, acquiring target influence factor data; determining a plurality of target influence factors based on the water quality parameters and the nitrification process of the to-be-monitored sewage; s2, establishing a soft measurement model; the input of the soft measurement model is the target influence factor, and the output of the soft measurement model is the concentration of nitrite nitrogen; s3, determining the concentration of nitrite nitrogen; according to the method, the target influence factor is firstly acquired by utilizing the sensor, and the nitrite concentration is calculated or predicted in real time by utilizing the target influence factor, so that the problems that monitoring equipment is expensive, the monitoring range is small, the monitoring is inaccurate and the monitoring equipment cannot directly monitor the nitrite in the seriously polluted wastewater in the nitrite monitoring in the prior art can be solved.

The invention discloses an aquaculture denitrification microcapsule as well as a preparation method and application thereof, and relates to the technical field of aquaculture and biology. The lactobacillus casei provided by the invention can secrete acid metabolites, and the acid metabolites can inhibit pathogenic vibrio, so that the occurrence of diseases in aquaculture can be reduced. Lactobacillus casei has the efficacy of efficiently degrading ammonia nitrogen and nitrite nitrogen in the aquaculture environment, and can continuously realize efficient cleaning of the aquaculture environment. In addition, the invention also provides a denitrification microcapsule which creates a more favorable microenvironment for the efficient denitrification strain, so that the repeated denitrification performance is more stably exerted, and the denitrification microcapsule can be repeatedly utilized.

The invention discloses a virulence factor gene relative abundance prediction model based on water quality physicochemical indexes, and relates to the technical field of ecological environment protection, and the virulence factor gene relative abundance prediction model comprises the following steps: taking a plurality of river water samples, obtaining virulence factor gene relative abundance of the river water samples, and obtaining water quality physicochemical index data of the river water samples; temperature, redox potential, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, total nitrogen, total dissolved solids, pH, conductivity, chlorophyll-a, chemical oxygen demand, dissolved oxygen, total organic carbon, total phosphorus, organic nitrogen, phosphate, carbon-nitrogen ratio and organic nitrogen / total nitrogen are taken as explanatory variables, and relative abundance of virulence factor genes is taken as a target variable. And establishing a regression model by adopting a GBM algorithm in an h2o packet in R software, and predicting the relative abundance of the virulence factor gene of the river water sample to be detected by utilizing the prediction model. The research provides a brand new method for predicting the relative abundance of the virulence factor gene and analyzing the environmental response of the virulence factor gene, and the method is suitable for identification and interpretation of a high-dimensional nonlinear ecosystem with complex interaction.

The present invention discloses a strain of Stutzerimonas kunmingensis and its application in water denitrification, relating to the field of biotechnology. The Stutzerimonas kunmingensis was deposited in the General Microbiology Center (CGMCC) of the China Culture Collection of Microorganisms on April 24, 2024, with a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and a deposit number of CGMCCNO.30427. The present invention isolated and obtained Stutzerimonas kunmingensis 9‑4 from a water sample of a crucian carp recirculating aquaculture system. The strain has a good removal effect on nitrate nitrogen, nitrite nitrogen and ammonia nitrogen in the water environment. At the same time, adding hematite under light conditions can significantly improve the removal rate of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen by the Stutzerimonas kunmingensis, which can be applied to the removal of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen in aquaculture water environment and aquaculture tail water.

The invention belongs to the field of environmental microorganisms, and discloses a high-temperature-resistant efficient nitrosation complex microbial inoculant and an application of the high-temperature-resistant efficient nitrosation complex microbial inoculant in aerobic composting of livestock and poultry manure, and the complex microbial inoculant comprises bacillus firmus CCTCC M 20241091, vinca bacillus CCTCC M 20241092, and achromobacter xylosus CCTCC M 2024620. The compound strain can accelerate conversion of ammonium nitrogen into nitrite nitrogen during aerobic composting of livestock and poultry manure, so that the amount of NH4 < + >-N converted into NH3 in the composting process is reduced, and nitrogen loss caused by volatilization of ammonia gas is reduced. The nitrosobacteria agent has the advantages of high conversion efficiency, simple operation and no secondary pollution, and is an effective method for reducing nitrogen loss and ammonia volatilization in the aerobic composting process.

The invention relates to the technical field of sewage treatment, in particular to an internal and external backflow automatic control system for sewage treatment. The system comprises an internal reflux system and an external reflux system, and the internal reflux system adjusts the frequency of internal reflux pumps or the number of started internal reflux pumps by monitoring the change of water quality parameters such as nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and dissolved oxygen so as to maintain the balance of nitrification and denitrification reactions; and the external reflux system adjusts the flow of an external reflux pump by monitoring the sludge concentration and the sludge level height so as to maintain the concentration and activity of microorganisms in the biological reaction tank. Besides, the system also adopts a mathematical model, a feedforward control strategy and a feedback control module, so that the response speed and the stability of the system are improved, the effective treatment of sewage is ensured, and the overall treatment efficiency and the effluent quality are improved.

The invention discloses a two-stage short-cut denitrification-anaerobic ammonia oxidation double-granule sludge device for sewage treatment. Comprising a nitrate nitrogen wastewater tank and a carbon source water tank which are arranged in parallel, a short-cut denitrification granular sludge reactor which is jointly connected with the nitrate nitrogen wastewater tank and the carbon source water tank, and an anaerobic ammonia oxidation granular sludge reactor which is connected with the short-cut denitrification granular sludge reactor in series, the short-cut denitrification granular sludge reactor comprises a short-cut denitrification tank body, a first water inlet is formed in the lower end of the short-cut denitrification tank body, the nitrate nitrogen wastewater tank is communicated with the first water inlet through a first water inlet pump, and the carbon source water tank is communicated with the first water inlet through a second water inlet pump; according to the invention, short-cut denitrification and anaerobic ammonia oxidation decoupling are realized, and adverse effects of organic matters on anaerobic ammonia oxidation are avoided; the whole set of device is a complete continuous flow process, and compared with the process in the prior art, the production rate of nitrite nitrogen is effectively improved, and a reaction substrate can be continuously and stably provided for anaerobic ammonia oxidation.

The invention belongs to the technical field of microorganisms, and particularly relates to a microbial composite flora with nitrogen removal capacity as well as a construction method and application of the microbial composite flora. The microbial composite flora with the nitrogen removal capability comprises mixed leissella luricola, leissella luricola, bacillus mucilaginosus, selaginella hydrocarbonoclada and rosinia aggresticus A S419 (Roseium aggresticus A S419), and the microbial composite flora with the nitrogen removal capability is prepared from mixed leissella luricola. The ratio of the viable counts of the mixed leptosticia parviflora to the viable counts of the leptosticia parviflora to the viable counts of the bacillus mucilaginosus to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the viable counts of the septosticia parviflora to the Research shows that the constructed microbial composite flora with the nitrogen removal capacity is a flora capable of efficiently removing nitrate nitrogen and nitrite nitrogen in high-salinity water in mariculture and the like, and a simple and effective operation method is provided for nitrogen removal of high-salinity wastewater.

The invention discloses SNP (Single Nucleotide Polymorphism) molecular markers of a Penaeus vannamei HIF-1alpha gene. The SNP molecular markers are respectively positioned at basic groups of the 67th site, the 119th site, the 133rd site, the 172nd site, the 340th site, the 409th site, the 437th site, the 474th site, the 577th site, the 647th site and the 749th site of a nucleotide sequence shown in a sequence 1 in a sequence table. The molecular marker is used as a functional marker of the nitrite-resistant character of the penaeus vannamei for breeding, specifically, genome DNA of muscular tissue of the penaeus vannamei to be detected is extracted, then the obtained genome DNA is used as a template for PCR amplification and purification of a PCR amplification product, then the obtained PCR amplification product is sequenced, and the content of the nitrite-resistant character of the penaeus vannamei is determined. The genotype of the molecular marker is determined, so that dominant genotype individuals are selected for breeding the penaeus vannamei boone, and a penaeus vannamei boone strain with good nitrite-resistant character can be obtained.

The invention discloses a heterotrophic nitrification-aerobic denitrification bacterium resistant to high ammonia nitrogen and high organic matters and application of the heterotrophic nitrification-aerobic denitrification bacterium in landfill leachate treatment. The heterotrophic nitrification-aerobic denitrification bacterium provided by the invention is Paracoccus pantotrophus HNAD-9, the strain is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on August 22, 2024, and the preservation number of the strain is CGMCC No.31698. The invention further discloses a preparation method of the heterotrophic nitrification-aerobic denitrification bacterium. According to the method, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen can be removed in the same heterotrophic and aerobic system at the same time, accumulation of nitrate nitrogen and nitrite nitrogen is avoided in the ammonia nitrogen removal process, and the method has important application prospects in the field of high-ammonia-nitrogen and high-organic-matter-concentration landfill leachate treatment.

The invention discloses a strain of Aeromonas bivalvae and application thereof. Aeromonas bivalvium ) was named R‑1, and was deposited in the General Microbiological Center of China Microbiological Culture Collection Committee on March 29, 2024, with the deposit number: CGMCC No. 30186. The bivalve Aeromonas provided by the present invention can effectively remove nitrite nitrogen, nitrate nitrogen and ammonia nitrogen in aquaculture tail water, with good removal effect, and can also be used to prepare shrimp feed, effectively improving the production speed of shrimp.

The application discloses a method for efficient denitrification and decarburization of kitchen waste and sludge co-fermentation anaerobic digestion effluent, which removes part of COD and ammonia nitrogen in the influent sludge by using a first SNAD, and maintains a certain amount of free ammonia of a certain concentration due to the high concentration of ammonia nitrogen and the high pH of the influent, so as to maintain the inhibition effect on nitrifying bacteria and ensure the conversion of part of the ammonia nitrogen into nitrite nitrogen, and the high activity of anaerobic ammonia oxidation bacteria is ensured in the first SNAD by limiting the aeration amount, so that the ammonia nitrogen and nitrite can be further removed; the effluent enters a nitrification tank, and the ammonia nitrogen is nitrified into nitrate nitrogen under the condition of nitrifying bacteria and sufficient aeration amount, and then enters a denitrification tank, and the denitrification process is carried out under the condition of heterotrophic denitrifying bacteria and sufficient carbon source, so that most of the nitrate nitrogen in the system is converted into nitrogen gas and removed, finally, the clear liquid is discharged after being filtered by an MBR membrane in a membrane tank, so as to achieve the purpose of efficient denitrification and decarburization of kitchen waste anaerobic digestion effluent.

A method for in-situ efficient enrichment of anaerobic ammonia oxidation bacteria, comprising: using anaerobic digestion sludge as inoculation sludge and using nitrate wastewater as an influent substrate, the inoculation sludge and the influent substrate reacting with each other under a light-proof, constant-temperature and closed condition so as to enrich anaerobic ammonia oxidation bacteria. After the anaerobic ammonia oxidation bacteria are enriched about 30 days by using the anaerobic digestion sludge as the inoculation sludge and the nitrate wastewater as the influent substrate, the number of functional genes HzsB of the anaerobic ammonia oxidation bacteria is increased by about 100 times than that in a control group (using a liquid mixture of ammonia nitrogen and nitrite nitrogen as an influent substrate). Thus, on one hand, there is no need to add extra ammonia nitrogen and nitrite nitrogen, and on the other hand, there is no need to regulate and control complex process parameters. Therefore, in-situ rapid and sustainable enrichment of anaerobic ammonia oxidation bacteria can be implemented in wastewater treatment plants simply by means of an economical and simple operation mode.

The invention relates to the technical field of water treatment, and discloses a sludge quality-divided reflux type aerobic-anoxic sulfur autotrophic activated sludge denitrification process. The process comprises the following steps: wastewater enters a sectional type aerobic tank, the wastewater enters the sectional type aerobic tank, organic matters are removed in a strengthened manner in a first section of aerobic tank, part of ammonia nitrogen is converted into nitrite nitrogen and nitrate nitrogen, and nitrification is strengthened in a second section of aerobic tank, so that the ammonia nitrogen is deeply removed; effluent of the sectional type aerobic tank enters an anoxic tank and is completely mixed with sulfur particles and activated sludge for sulfur autotrophic denitrification; effluent of the anoxic tank enters a secondary sedimentation tank for sedimentation, supernate is discharged, and bottom mud enters a hydrocyclone to be divided into heavy sludge and light sludge; the heavy sludge flows back to the front end of the anoxic tank, the light sludge flows back to the front end of the sectional aerobic tank, and the remaining sludge is discharged. According to the invention, sulfur autotrophic denitrification is deeply integrated into an activated sludge system, so that high-efficiency nitrogen removal without carbon source and internal reflux is realized.

The invention relates to the field of fish and vegetable comprehensive cultivation, in particular to a photovoltaic fish and vegetable symbiotic system which comprises a photovoltaic system, a fishery breeding system and a circulating purification system, a fishpond is arranged below a photovoltaic panel, net cages are arranged in the fishpond for fish breeding, the circulating purification system is arranged around the fishpond, and water is pumped in the fishpond through a pump station; water quality purification and water quality maintenance are carried out on the pumped water through a precipitation filtration system, a microfilter, a hydroponic vegetable system and an ultraviolet disinfection system in sequence, so that the water body in the pond is ensured to fully and circularly flow, and the concentration of chemical substances such as ammonia nitrogen, nitrite nitrogen and the like which are harmful to fishes is reduced. Through the synergistic effect of all parts of the photovoltaic fish and vegetable symbiotic system, the problem that the water quality in the aquaculture environment under the photovoltaic panel is prone to deterioration is effectively solved, and the aquaculture benefits are improved.

The invention discloses a method for realizing long-acting stable shortcut nitrification and denitrification phosphorus removal based on sludge sidestream treatment, and belongs to the field of biological sewage treatment. After the short-cut nitrification process, the denitrification process, the phosphorus removal process and the carbon removal process of sewage in the reactor are completed, activated sludge is divided into three directions, one part of the activated sludge is discharged to maintain the sludge age, the other part of the activated sludge continues to be subjected to the nitrogen and phosphorus removal process in the reactor, and a small part of the sludge with excellent denitrification and phosphorus removal performance is reserved to flow back to an independent storage pool. In the later period of operation, after the cell activity in the activated sludge is damaged due to accumulation of high-concentration nitrite nitrogen, the activated sludge retained in the early stage flows back to the reactor, so that the denitrifying phosphorus removal performance of the sludge is enhanced, and efficient and stable nitrogen and phosphorus removal is realized. The method realizes synchronous deep removal of nitrogen and phosphorus in sewage, is suitable for automatic operation, and is convenient for realizing accurate control and optimal management of the process.

The invention discloses marinobacter hydrocarbonoclastici SN-1 and a culture method and application thereof, the marinobacter hydrocarbonoclastici SN-1 is marinobacter hydrocarbonoclastici SN-1, is preserved in China Center for Type Culture Collection on August 7, 2024, is located in Wuhan University, Wuhan, China, and has a preservation number of CCTCC NO: M 20241755, and the preservation number of the marinobacter hydrocarbonoclastici SN-1 is CCTCC NO: M 20241755. The invention also discloses application of the marinobacter hydrocarbonoides SN-1 or the microbial inoculum in degradation of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and / or active phosphate concentration in seawater. The seawater body is a mariculture water body, mariculture tail water or an offshore water body, the salinity of the seawater body is 10-40, and the inoculum size of the mariculture SN-1 is 2%. The marinobacter hydrocarbonoides SN-1 disclosed by the invention can remove ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total nitrogen, active phosphate and total phosphorus in a seawater water body in an aerobic environment with relatively high salinity, has no toxic effect on aquatic animals, and can be applied to improvement and ecological restoration of a seawater water environment.

The invention discloses a water quality parameter measurement method driven by a multilayer deep learning model, which realizes high-precision measurement of key water quality parameters such as COD (Chemical Oxygen Demand), total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), nitrite nitrogen (NO3-N), ammonia nitrogen (NH3-N), turbidity and chromaticity by fusing CNN (convolutional kernel 3 * 1 and kernel number 32 / 64), BiLSTM (hidden nodes 30-60) and Attention mechanism and combining WOA hyper-parameter optimization (learning rate 0.001-0.01). According to the method, Savitzky-Golay filtering (window 11, order 3) denoising is adopted, data quality is enhanced through normalization preprocessing, model robustness is improved through 10-fold cross validation and an Adam optimizer, the problem that a traditional model is poor in adaptability to complex spectrum time sequence data is solved, and the method is suitable for rapid online monitoring of water quality.

The invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and application thereof, the heterotrophic-aerobic denitrifying bacterium C-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC), is classified and named as brevundimonas, has the preservation number of CGMCC No.31729, and is preserved on August 23, 2024. The heterotrophic-aerobic denitrifying bacterium C-3 has the advantages that the preservation number is CGMCC No.31729, and the preservation date is 2024. The heterotrophic-aerobic denitrifying brevundimonas vesicularis C-3 (Brevundimonas vesicularis C-3) provided by the invention takes sodium acetate as a carbon source, and under the aerobic conditions that the C / N mass ratio is 10, the pH value is 7, the temperature is 30 DEG C and the rotating speed is 200r / min, a relatively good nitrate nitrogen removal effect is achieved, and the removal effect is 100%; in actual aquaculture tail water, nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total nitrogen can be well removed; meanwhile, the strain has high safety and has good application value in treatment of aquaculture tail water.

The utility model relates to a rural sewage treatment system with low carbon emission. The method is suitable for the environment-friendly fields of sewage treatment, pollution reduction and carbon reduction. The technical scheme adopted by the utility model is that the system comprises a water inlet pool; the anaerobic unit is used for providing an anaerobic environment; the aerobic I unit is used for providing an aerobic environment with the dissolved oxygen concentration higher than a preset value; the anoxic I unit is used for providing an anoxic environment; the anoxic II unit is used for providing an anoxic environment so as to remove nitrite nitrogen, nitrate nitrogen and ammonia nitrogen through denitrification and anaerobic ammonia oxidation reaction; the aerobic II unit is used for providing an aerobic environment with the dissolved oxygen concentration higher than a preset value, so that aerobic phosphorus removal and ammonia nitrogen oxidation reaction are facilitated; the secondary sedimentation tank unit is used for mud-water separation, and bottom sludge flows back to the anaerobic unit; the water outlet tank is used for receiving clarified effluent separated by the secondary sedimentation tank unit; and the first dosing unit is arranged corresponding to the anaerobic unit and is used for adding nano ferroferric oxide into the anaerobic unit.

The invention discloses a method for in-situ enrichment of anaerobic ammonia oxidizing bacteria, a bio-membrane reactor is adopted, and a carrier of a bio-membrane is made of a material with good conductivity and biological affinity; the direct-current power supply continuously outputs weak voltage below 1.0 V; preparing special wastewater as inlet water of the reactor; sludge containing anaerobic ammonium oxidation bacteria is selected as inoculation sludge, the hydraulic retention time of the reactor is controlled, microorganisms in the reactor mainly grow on a carrier in an attached mode to form a biological membrane, and suspended sludge is eliminated as much as possible; aeration is performed on the reactor, and water enters and exits the reactor continuously. According to the method, the anaerobic ammonium oxidation bacteria are enriched under the micro-aerobic condition, the nitrogen source is only ammonia nitrogen, nitrite nitrogen is generated in the ammonia nitrogen conversion process instead of being supplied externally, the normal form of anaerobic ammonium oxidation enrichment culture is innovated through the method, and the anaerobic ammonium oxidation enrichment culture efficiency is improved. The problem that anaerobic ammonia oxidizing bacteria cannot be enriched due to lack of external supply of nitrite nitrogen in the actual wastewater treatment process is effectively solved.

The invention discloses a device and a method for cooperatively treating domestic sewage and nitrate wastewater by coupling heterotrophic denitrification and sulfur autotrophic denitrification with anaerobic ammonia oxidation, and belongs to the field of biological denitrification of sewage. The device comprises a water inlet tank, a sequencing batch reactor (SBR), a peristaltic pump, a drain valve, a water outlet tank and the like. The method comprises the following steps: (1) adding sulfur particles into a first SBR (Sequencing Batch Reactor), introducing nitrate wastewater and domestic sewage into the SBR, converting nitrate nitrogen into nitrogen through sulfur autotrophic denitrification under an anaerobic condition, and reducing the nitrate nitrogen into nitrite nitrogen when COD (Chemical Oxygen Demand) comes from the domestic sewage; and (2) adjusting the proportion of ammonia nitrogen and nitrite in the effluent of the first SBR, and stably removing ammonia nitrogen and nitrite through anaerobic ammonia oxidation. In the two-stage system, 100% of aeration and external carbon sources are saved, and a new thought is provided for synergistic denitrification of pure autotrophic systems of industrial wastewater and domestic sewage.