965 results about "Nitrite nitrogen" patented technology

The invention belongs to the technical fields of environmental engineering and bioengineering and particularly relates to a sphingomonas strain and applications thereof in the aspects of shortcut nitrification-denitrification of nitrogen-containing industrial waste water and domestic sewage and treatment of a polluted water source. The sphingomonas strain is separated from a Taihu water in China,is a local strain and has high safety; and the strain can be grown in a basic medium, wherein in the basic medium, CO2 is used as a carbon source and energy or CO2 and an organism are used as a mixedcarbon source and energy, and ammonia nitrogen or nitrate nitrogen is used as a nitrogen source. The bacterium liquid, the dormancy cell and the immobilized strain of the sphingomonas can decompose ammonia nitrogen into nitrite nitrogen and simultaneously can decompose the ammonia nitrogen into nitrogen, can be used as denitrification microorganism for converting the ammonia nitrogen into the nitrite nitrogen and the nitrogen, thereby achieving the shortcut nitrification-denitrification. The strain can rapidly decompose the ammonia nitrogen and is suitable for treating the nitrogen-containingindustrial waste water and domestic sewage as well as polluted water source.

The invention relates to a bacteria strain for short-cut denitrification and an application thereof. The short-cut denitrification bacteria strain is arthrobacter creatinolyticus FDN-1 and is preserved in Ordinary Microorganism Center of China Committee for Culture Collection of Microorganisms on March 11, 2010, and the preservation number is CGMCC No. 3657. The bacteria strain can directly adopt nitrite nitrogen as a substrate to complete the short-cut denitrification process. When the arthrobacter creatinolyticus FDN-1 is used for processing ammonia-contained waste water, the technique is simple, high denitrification activity can be still obtained under the condition that low-concentration organic carbon source exists, and at the same time the high-concentration organic carbon source can be withstood. When the bacteria strain is in use, the system is rapid to start, and a vast application prospect can be realized in the denitrification treatment process of different types of waste water.

The invention discloses a device and a method for denitrification of single stage autotroph in low-cellulose nitrate (CN) high-ammonia nitrogen waste water. The device comprises a raw water tank, a water inlet pump, a reactor, a secondary sedimentation tank and a sludge reflux pump, wherein overcurrent holes are arranged in the water flow direction of the reactor in an up-and-down alternative form and connected with various grid chambers, an anoxic zone grid chamber is arranged at the front end of the reactor, and an aerobic zone grid chamber is arranged at the back end of the reactor; the anoxic zone grid chamber is provided with a stirrer and an agitator blade; the aerobic zone grid chamber is provided with the stirrer, the agitator blade, an aeration riser pipe and an intermediate perforated clapboard; and the aeration riser pipe is internally provided with an aeration head, and sponge filling material is filled below the intermediate perforated clapboard, wherein filling ratio is 30-50%. In the method, shortcut nitrification is achieved above the aerobic zone through low dissolved oxygen (DO is 0.5 <-1>mg/L) and free ammonia (FA) inhibition so that ammonia nitrogen is converted to nitrite nitrogen; and anoxicammoxidation biomembrane acts on the lower part of the aerobic zone, the nitrite nitrogen and ammonia nitrogen are converted to nitrogen, thereby achieving denitrification of autotroph. The method has the advantages of low oxygen consumption, less sludge output and no extra carbon source.

The invention discloses a preparation method and an application of immobilized bacteria for improving the denitrification efficiency of a constructed wetland. 1. The preparation steps are as follows: 1) screening and culturing aerobic denitrification strains which are screened out of the constructed wetland in a thermotank; 2) selecting aerobic denitrification bacteria in slope conservation, inoculating into a sterile enrichment culture medium and aerating to get bacterial suspension; 3) using sodium alginate-polyvinyl alcohol to embed so as to get the immobilized aerobic denitrification bacteria; and 4) taking pellets of the aerobic denitrification bacteria out of a refrigerator, washing with double distilled water or physiological saline, soaking in the physiological saline, and aerating to activate the immobilized pellets. 2. An application of the immobilized bacteria in the constructed wetland is as follows: filling the immobilized pellets into a simulation column of the constructed wetland for performing waste water denitrification treatment. The preparation method of the immobilized bacteria is simple, the production cost is low, the application in the industrial waste water treatment is easy, the denitrification efficiency is high, the accumulation amount of nitrite nitrogen is low, and the service life is long.

The invention relates to a method for quickly starting an oxygen-poor ammonia oxidizing biofilter, and relates to a method for biological denitrification of waste water, which comprises the following steps of: (1) inoculating an anaerobic denitrification biomembrane biofilter, and after a denitrification biomembrane is cultured, converting the anaerobic denitrification biomembrane biofilter into an oxygen-poor environment for cultivating oxygen-poor ammonia oxidizing bacteria; (2) taking inorganic carbon NaHCO3 as a carbon source, taking NH4Cl as a nitrogen source of ammonia nitrogen, taking NaNO2 as the nitrogen source of nitrite nitrogen, taking KH2PO4 as a phosphorus source, and controlling the pH value to be alkalescent, wherein the concentration is low-matrix, and the temperature is room temperature; adding hydroxylamine, and inducing and inoculating sludge to be converted towards an oxygen-poor ammonia oxidizing biomembrane; and (3) taking the biofilter as a reactor, and taking ceramsite as filter materials. In the method, the oxygen-poor ammonia oxidizing bacteria are applied to the sewage denitrifying technology to realize the shortest denitrification of nitrogen, so the problems of a large number of required carbon sources and neutralizers, large energy consumption and large sludge producing amount in the conventional biological denitrifying technology are solved.

The invention relates to a bacterial strain used for short-cut denitrification for nitrogen removal and its application. The bacterial strain used for short-cut denitrification for nitrogen removal is flavobacterium mizutaii FDN-2, which is preserved in China general microbiological culture collection center on March 11, 2012 and has a preservation number of CGMCC NO.3659. The bacterial strain can directly utilize nitrite nitrogen as a substrate to complete a short-cut denitrification process. When treating ammonia-containing wastewater with the flavobacterium mizutaii FDN-2 of the invention,the process is simple, and the flavobacterium mizutaii FDN-2 can tolerate a high concentration organic carbon source. When the flavobacterium mizutaii FDN-2 in the invention is put into use, a systemcan be rapidly initiated, so that the flavobacterium mizutaii FDN-2 boasts very broad application prospects in various nitrogen removal processes of wastewater.

The invention relates to a Kocuria palustris strain and applications thereof. The strain is Kocuria palustris FSDN-A, and is preserved in the China General Microbiological Culture Collection Center on July 14, 2011, wherein a preservation number is CGMCCNO.5061. According to the present invention, the strain can adopt nitrite nitrogen or nitrate nitrogen as a substrate to complete a denitrification process; when the Kocuria palustris FSDN-A is adopted to treat ammonia-containing wastewater, characteristics of simple process, high denitrogenation activity, and high concentration organic carbon source tolerance are provided; after the strain is poured, the system is rapidly started; and the strain has broad application prospects in wastewater denitrogenation treatment processes, and is especially suitable for treatment of wastewater containing nitrogen oxides and organic pollutants.

The invention discloses a cultivation method of anammox granular sludge, and belongs to the field of water treatment. The cultivation method solves the technical problems that the existing high-efficiency anammox granular sludge is difficult to cultivate, and the cultivation time is long. The method comprises the following steps that 1, aerobic granular sludge and anammox seed sludge are simultaneously inoculated into an EGSB (expanded granular sludge bed) reactor; 2, nitrogenous simulated waste water is supplied to the EGSB reactor, the hydraulic retention time is kept for 12h, and concentrations of ammonia nitrogen and nitrite nitrogen in intake water are gradually increased until successful start when the synchronous removal rate of ammonia nitrogen and nitrite nitrogen is above 80%; and 3, intake water concentrations of ammonia nitrogen and nitrite nitrogen are kept stable, the hydraulic retention time is gradually shortened up to 2.6h when the synchronous removal rate of ammonia nitrogen and nitrite nitrogen is above 80%, and then the cultivation of the anammox granular sludge is completed. With the method, the reactor can be started successfully within about 20 days, and the high-efficiency anammox granular sludge can be obtained within about 89 days.

The invention discloses a UBF reactor used for coupled denitriding via anaerobic ammonia oxidation-sulfur-based autotrophic denitrification, and a system and a denitriding method thereof, and belongs to the technical field of waste water processing. The denitriding method is suitable to be used for processing high mmonia nitrogen low C/N waste water, partial nitrification is used for providing all reaction substrates of anaerobic ammonia oxidation, marcasite is used for providing electron donors of sulfur-based autotrophic denitrification; porous limestone is taken as a supporting framework of a limestone filling layer; and nitrite nitrogen generated via nitrosation, and nitrate nitrogen generated via nitrification and anaerobic ammonia oxidation are taken as electron acceptors. The denitriding method is high in ammonium-nitrogen removal rate; operation stability is high; compared with conventional nitration-denitrification denitriding method, the denitriding method possesses following advantages: no extra organic matter is added as the electron donor, marcasite is ued for providing the electron donors of sulfur-based autotrophic denitrification bacteria, cost is low, controllability is high, effluent is stable, sludge yield is low, and energy consumption is low.

The invention relates to Bacillus amyloliquefaciens and application thereof on aquiculture. The bacterial strain is separated from the substrate sludge of a polluted brook, is identified as the Bacillus amyloliquefaciens and is collected in Microscobial Culture Collection of China. The bacterial strain can normally grow within the temperature of 20 to 45 DEG C and within the pH value of 5.0 to 10.0; the bacterial strain can reach the fermentation terminal point when being inoculated on a broth culture medium the initial pH value of which is 7.0 according to inoculum concentration of 1:20 and cultured for 18 hours at the speed of 150r.min-1 at the temperature of 39 DEG C. The invention is characterized in that the bacterial strain can enable the content of nitrate nitrogen, nitrite nitrogen, COD and the like in a culture water body to be obviously lowered, has no harm to people and livestock and can be produced on a large scale for improving the aquiculture environment. The Bacillus amyloliquefaciens has obvious degradation function on main pollutants in a simulate culture water body and can be made into a micro-ecology preparation for purifying the culture water body.

The invention relates to multistage anaerobic, aerobic and anoxic dephosphorization and denitrogenation technology, which is mostly suitable for treating urban domestic sewage. The sewage is divided into two parts, namely approximately 30 percent of the sewage is mixed with return sludge and enters into an anaerobic tank, and most of the sewage exceeds the anaerobic tank and directly enters into an anoxic tank for denitrogenation in a multipoint mode; after denitrogenation is completed, the mixed solution is mixed with a small part of the sewage and enters into a secondary settling tank for sludge-water separation; and denitrogenation reaction is continuously completed during sludge-water separation; and the return sludge and nitrite nitrogen and nitrate nitrogen of discharged water are reduced, and residual sludge is discharged regularly. The multistage anaerobic, aerobic and anoxic dephosphorization and denitrogenation technology has high phosphor and nitrogen removal rate, reduces the capacity of a structure, saves investment, fully utilizes carbon sources and simultaneously reduces direct oxygen consumption because the sewage does not directly enter into an aerobic tank and then the carbon sources in the sewage do not directly contact oxygen, and improves the catch rate of air bubbles and obviously saves the aeration cost due to ultrahigh sludge concentration in the aerobic tank under the condition of blast aeration.

The invention relates to a rotating biological-cathode microbiological fuel cell and a sewage treatment method thereof, and belongs to the technical field of sewage treatment and resource recycling in environment engineering. The invention is characterized in that a cathode is made of carbon fibers and fixed to a circular ring. The outer layer of the rotating cathode biomembrane mainly comprises aerobic nitrifiers for completing the short-range nitrification of ammonia nitrogen. In the inner layer of the cathode biomembrane, nitrite nitrogen and nitrate nitrogen are used as electron acceptors, an electrode is an electron donor and used for denitrification. The structure of a reactor realizes controllable rotation speed of the cathode, controllable distance between the cathode and the anode, and controllable submerging proportion of the cathode biomembrane, and has the advantages of flexible operation, and convenient regulation and control. The rotating biological-cathode microbiological fuel cell can realize control on dissolved oxygen in a single-chamber reactor; and thus, the invention reduces the internal resistance of the cell, accelerates the biomembrane renewing, realizes the short-range nitrification and denitrification, synchronously completes decarburization and denitrification with low consumption and high efficiency, and extracts chemical energy from pollutants to form electric energy output.

The invention discloses a culture method of a nitrosomonas sp. flora and a treatment method of waste water containing ammonia nitrogen. The ammonia nitrogen concentration and the pH value of a matrix are increased during an enrichment process, and free NH3 with higher concentration is maintained, thereby gradually achieving the purposes of eliminating nitrobacter sp. and enriching nitrosomonas sp. and obtaining a nitrosomonas sp. flora. Then, the obtained nitrosomonas sp. flora is taken as seeds, the expanded culture is carried out by utilizing self-prepared ammonia nitrogen waste water or high ammonia nitrogen waste water as a culture liquid, the nitrosomonas sp. flora after the expanded culture is inoculated into a biochemical treatment pool for improving the removal efficiency of ammonia nitrogen by biochemical treatment, or the nitrosomonas sp. flora after the expanded culture is directly used for removing the ammonia nitrogen of the ammonia nitrogen waste water, and the optimal proposal is used in the aerobic nitrification stage of the shortcut nitrification-denitrification process for accumulating nitrite nitrogen as an electron acceptor for denitrification directly. The method has simple process and outstanding effect of treating the waste water containing the ammonia nitrogen.

The invention discloses a low-carbon urban sewage biological phosphorus removal and autotrophic biological nitrogen removal device and a low-carbon urban sewage biological phosphorus removal and autotrophic biological nitrogen removal method. The device comprises a raw water tank, a biological phosphorus removal and half shortcut nitrification reactor, a secondary sedimentation tank, an intermediate water tank and an anammox reactor, wherein the biological phosphorus removal and half shortcut nitrification reactor is a university of cape town (UCT) reactor without internal returning of digestive fluid; the secondary secondary sedimentation tank is a radial-flow sedimentation tank; and the anammox reactor is an upflow anaerobic sludge blanket (UASB) reactor. The method comprises: introducing urban sewage first to the anaerobic zone of the biological phosphorus removal and half shortcut nitrification reactor for phosphorus release, then to an anoxic zone of the biological phosphorus removal and half shortcut nitrification reactor for denitrification and phosphorus absorption and finally to an aerobic zone for aerobic phosphorus absorption and half shortcut nitrification; subjecting mixed liquid containing ammonia nitrogen and nitrite nitrogen to sludge and water separation in the secondary sedimentation tank; and introducing the water to the anammox UASB reactor for autotrophic biological nitrogen removal under anammox action. When the method is used, process aeration can be reduced, energy consumption can be reduced, and carbon source is saved. The method is suitable for treating low-carbon sewage.

The invention discloses an ecotype enclosed method for culturing fish through circulation water. Natural seawater, underground salt water, underground fresh water and geothermal water are mainly utilized and mixed, and an industrial culture foundation for producing pollution-free healthy seafood is built and used. A solid-liquid separation purification device is used for replacing sand filtration of a biological filter, ammonia nitrogen and nitrite nitrogen are degraded after water passing through a biological film on biological filler in the biological filter, and the total purification rate of water body particles is over 99%. An aeration pond is arranged to conduct aeration and oxygenation on a culture water body to enable the content of dissolved oxygen in water in a fish culture pond to be kept at 8-14mg/L, finally the water body is sterilized through ultraviolet light, clean ecological water is input into the fish culture pond, and the return water in the fish culture pond is purified repeatedly and utilized in a circulating mode. Sewage discharged from a culture system is purified by an open-air pond and flows back to a water storage sedimentation pond to achieve zero-discharge complete enclosed circulation water factory-like fish culture.

In the invention discloses an energy-saving treatment method of high strength ammonia wastewater, which comprises procedures of pre-processing, short-cut nitrification, micro-electrolysis and after-treatment. The method is characterized in that firstly wastewater is preprocessed to transform Kjeldahl nitrogen into amino nitrogen, then the wastewater enters a main body of the process to be processed. In a short-cut nitrification tank, the amino nitrogen in the wastewater is nitrated and controlled to a nitrite nitrogen stage, then a micro-electrolysis reactor is employed to replace anaerobic denitrification or ammonoxidation process to be denitrified, the nitrite nitrogen and organic pollutants in the wastewater are highly effectively degraded and are further subsequently processed by employing biological method and physicochemical oxidation method, and outlet water of the wastewater reaches standards. The method makes full use of water quality of raw water, the additional medicament consumption is saved, the process flow is shortened, and the process operation is simplified. Compared with the conventional denitrification process, the denitrification rate is increased, the total nitrogen removal rate reaches 60%-75%, and the treatment effect of degrading hardly degradable organics is remarkably increased. The treatment cost of wastewater per ton by employing novel method can be reduced by 30-50%.

The invention relates to a method for the anaerobic ammonia oxidation treatment of low-concentration ammonia nitrogen wastewater. The method is characterized in that sludge containing anaerobic bacteria is inoculated into an anaerobic traverse baffle reactor under an anaerobic condition; the alkalescent ammonia nitrogen wastewater with 10 to 20mg/L of the concentrations of ammonia nitrogen and nitrite nitrogen enters the anaerobic traverse baffle reactor by an inlet water storage bottle, and a constant-current peristaltic pump is used for controlling the hydraulic stay time to be from 24 hours to 48 hours and the reaction temperature to be from 25 DEG C to 30 DEG C; if anaerobic ammonium oxidation bacteria become dominant flora and both the removal rates of the ammonia nitrogen and nitrite nitrogen reach 80 percent for 5 days, the anaerobic traverse baffle reactor is successfully started; then, other low-concentration ammonia nitrogen wastewater enters the anaerobic traverse baffle reactor by the inlet water storage bottle, and the hydraulic stay time is controlled to be from 12 hours to 48 hours; and finally, both the removal rates of the ammonia nitrogen and the nitrite nitrogen can reach about 80 percent.

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.

This invention relates to a kind of photosynthetic bacterium that has a high assimilation utility to nitrite nitrogen. More specifically, this invention provides a Rhodopseudomonas palustris strain that can assimilate nitrite nitrogen, and its r ejuvenation, preservation, biological detection and application. The photosynthetic bacterium is a kind of biological water purifier and biological fertilizer, and can be used in controlling the content of nitrite nitrogen in water body for aquaculture, improve environmental pollution, and eliminate cinnamic acid and benzoic acid. The photosynthetic bacterium can be used as nitrogen-fixing biological fertilizer and cucumber growth promoter.

The invention provides a method for cultivating anaerobic ammonium oxidation granular sludge. The method comprises the steps of: mixing and putting two or three kinds of anaerobic methanogenic granular sludge, nitrification aerobic granular sludge and hypoxia denitrification granular sludge into an upflow anaerobic reactor; pumping simulate waste water containing ammonia nitrogen and nitrite nitrogen to start; keeping the temperature of 25 to 35 DEG C in the reactor and the pH value of 7.5 to 8.0 without dissolved oxygen, and keeping hydraulic detention time of 8 to 24 hours; and stabilizing for a period of time to obtain the anaerobic ammonium oxidation granular sludge from the reactor. The invention has the advantages of having good hydraulic performance and microbial activity of the anaerobic ammonium oxidation granular sludge cultivated by the method, and greatly improving the operation effectiveness and the operation stability of the reactor.

The invention discloses a rapid culture method of simultaneous desulfidation and denitrogenation granular sludge. The method is characterized in that an upflow anaerobic sludge bed reactor is adopted, anaerobic ammoxidation granular sludge and methanogenesis granular sludge are adopted as inoculation sludge, model wastewater containing ammonia nitrogen and nitrite nitrogen is adopted as inlet water, and the method comprises the following steps: adding sulfide into the model wastewater, adding inorganic salts and trace elements for maintaining the growth of microbes, gradually improving the concentrations of matrixes in the inlet water and/or shortening the hydraulic retention time in order to adjust the running of the reactor, and culturing under anaerobic and shady conditions at 30-37DEG C under the pH value of the inlet water of 7.5-8.0. The sludge obtained in the invention has the advantages of obvious granularity, strong impact resistance, high processing efficiency, saving of the occupied are through cooperating with a UASB reactor, simplification of the desulfidation and denitrogenation process, and low investment and running cost, and is suitable for treating wastewater containing ammonia nitrogen, nitrite nitrogen and sulfides of the light industry, the pharmacy industry, the oil refining industry and the leather making industry.

The invention provides a method for achieving partial short-cut nitrification-Anammox/denitrification of sewage by using sludge fermentation materials, and belongs to the field of treatment of sewagesludge. A device used by the method comprises a raw water tank, an SBR reactor, a sludge fermentation material storage tank and a residual sludge fermentation tank. The method comprises mixing residual sludge fermentation materials with domestic sewage for treating, and achieving the short-cut nitrification by using the sludge fermentation materials, wherein the inhibition of the sludge fermentation materials on nitrite oxidizing bacteria is far greater than the inhibition of the sludge fermentation materials on ammonia oxidizing bacteria. The method is specifically characterized in that in ananaerobic phase, polyphosphate accumulating bacteria are used for releasing phosphorus by using the sludge fermentation materials and carbon sources in raw water, and storing the inner carbon sources, in an aerobic phase, the partial short-cut nitrification is achieved through real-time control on DO and pH, in an anoxic phase, anaerobic ammonia oxidizing bacteria are used for converting ammonianitrogen and nitrite nitrogen into nitrogen and nitric nitrogen, and heterotrophic bacteria are used for reducing residual nitrite nitrogen and the generated nitric nitrogen into nitrogen by using theinner carbon sources. Through the method, the aeration intensity is reduced; meanwhile, the deep denitrification of the domestic sewage with a low C/N ratio and the reduction of exogenous sludge areachieved.

The invention discloses a device and a method capable of realizing simultaneous phosphorus and nitrogen removal from sewage in a low C/N ratio by coupling enhanced biological phosphorus removal with simultaneous shortcut nitrification and denitrification and belongs to the field of biological sewage treatment. After entering an SBR coupling enhanced biological phosphorus removal with simultaneous shortcut nitrification and denitrification, sewage is first subjected to anaerobic stirring, and GAOs (glycogen-accumulating organisms) and PAOs (phosphorus-accumulating organisms) absorb organic carbon sources from the sewage and synthesize an internal carbon source PHA (polyhydroxyalkanoates) to be stored in bodies; then the sewage is subjected to low-oxygen aeration stirring, AOB (ammonia oxidizing bacteria) play a role in shortcut nitrification to convert ammonia nitrogen into nitrite nitrogen, and the PAOs decompose the PHA for aerobic phosphorus accumulation so as to create a hypoxic microenvironment beneficial to that the GAOs and the PAOs use the nitrite nitrogen produced during shortcut nitrification for endogenous denitrification and denitrifying phosphorus removal respectively. The method coupling enhanced biological phosphorus removal with simultaneous shortcut nitrification and endogenous denitrification is capable of realizing simultaneous phosphorus and nitrogen removal from the sewage in the low C/N ratio without addition of a carbon source, so that oxygen consumption and energy consumption are reduced and urban sewage treatment costs are saved.