Staphylococcus cohnii and applications thereof

A technology of staphylococcus and bacterial strains, applied in the field of environmental microorganisms, to achieve broad application prospects, fast start-up, and simple process effects

Active Publication Date: 2013-05-15
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on Staphylococcus used to treat nitrite nitrogen in industrial wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Enrichment culture and isolation and purification of bacteria

[0023] Enrichment culture of denitrifying bacteria:

[0024] The activated sludge in the nitrification tank of the sewage treatment plant is taken, and the medium containing nitrite is used for enrichment and cultivation under low dissolved oxygen conditions for a certain period of time to obtain denitrification activated sludge. During the enrichment process, fresh culture medium was replenished in due course.

[0025] Enriched medium formula: NaNO 2 , methanol, also contains Fe 2＋ , Mg 2＋ 、K ＋ , Ca 2＋ four metal cations. The culture conditions are: temperature 30°C; pH 8.2, DO (dissolved oxygen) 0.2-1.0 mg·L –1 .

[0026] The molar configuration ratio of the four metal cations is 1:(4-8):(5-15):(1-5). During the cultivation process, a growth promoter with a total metal cation concentration of 0.15 mol / L was added every day, and the added amount was 0.1% of the volume of the material in ...

Embodiment 2

[0030] Example 2 Investigation of denitrification ability of bacterial strains of the present invention

[0031] First, a single colony FSDN-C was picked from the plate and inoculated into beef extract peptone medium at 30°C and 120r / min on a shaker for 48 hours. After the bacterial suspension was centrifuged, it was transferred to the sodium nitrite-methanol culture solution. Under the same conditions, two methods of batch water exchange and batch feeding were adopted to investigate the denitrification ability. Table 1 shows the simultaneous removal effect of bacterial strains of the present invention on nitrous nitrogen and COD under the condition of batch water change. From the data in the table, in the process of increasing the total nitrogen concentration of influent from 200mg / L to 469mg / L, the total nitrogen removal rate is always greater than 88.63%, and in the process of increasing the COD concentration of influent from 2728mg / L to 4149mg / L, COD removal rate is great...

Embodiment 3

[0033] Embodiment 3 Strain of the present invention treats wastewater from a refinery

[0034] Take a certain amount of bacterial suspension obtained in Example 2 and inoculate according to the inoculum size of 15% (volume), at a temperature of 28°C, a pH of 8.3, and a DO (dissolved oxygen) of 1.2mg L – 1 Under certain conditions, the effluent of an oil refinery wastewater containing alkali slag after aeration treatment is treated, and the inorganic salt content is 25g / L, NO 2 - -N average 120mg / L, COD average 800 mg / L. After treatment, the effluent NO 2 - -N and COD average concentrations were 6.4mg / L and 61mg / L, NO 2 - The average removal rates of -N and COD were 94.7% and 92.4%, respectively.

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Abstract

The invention relates to a strain of Staphylococcus cohnii, and applications thereof. The strain is Staphylococcus cohnii FSDN-C, and is preserved in the China General Microbiological Culture Collection Center on July 14, 2011, wherein a preservation number is CGMCCNO.5062. According to the present invention, the strain can adopt nitrite nitrogen as a substrate to complete short time denitrification denitrogenation, wherein organic pollutants can be removed during denitrogenation; when the Staphylococcus cohnii FSDN-C is adopted to treat ammonia-containing wastewater, characteristics of simple process, and high concentration organic carbon source tolerance are provided; after the strain is poured, the system is rapidly started; and the strain has broad application prospects in various wastewater denitrogenation treatment processes.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a high-efficiency nitrous acid type (short-range denitrification) denitrification bacterial strain. The denitrification bacterial strain of the invention can carry out denitrification with nitrous nitrogen as a nitrogen source, and is especially suitable for denitrification in the process of short-range nitrification and denitrification of ammonia-containing wastewater. Background technique [0002] The traditional biological denitrification process mainly includes two processes of nitrification and denitrification. Nitrification is the premise of the denitrification process, and the denitrification process can truly remove nitrogen-containing compounds in wastewater. Because nitrifying bacteria have strong aerobic properties, the nitrification process is aerobic; while denitrifying bacteria are generally facultative bacteria, which use oxygen as an electro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/44
Inventor 高会杰孙丹凤张鹏韩建华
Owner CHINA PETROLEUM & CHEM CORP
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