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A Strain of Enterobacter cloacae and Its Application

An Enterobacter cloacae and base sequence technology, applied in the field of environmental microorganisms, can solve the problems of incomplete research on aerobic denitrifying bacteria, and the total nitrogen removal rate is less than 50%, and achieves good organic carbon removal ability and extreme tolerance. good effect

Inactive Publication Date: 2018-05-15
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] "Establishment of a new screening method for aerobic denitrifying bacteria and discovery of new strains", Kong Qingxin, "Journal of Applied and Environmental Biology", 2005.04 published: using intermittent aeration enrichment and potassium cyanide selection medium to screen A strain of Pseudomonas with aerobic denitrification function ( Pseudomonas sp.) Y2-1-1, this strain uses sodium citrate as carbon source and potassium nitrate as nitrogen source to reduce nitrate nitrogen from 282.0 mg / L to 148.2 mg / L within 5 days under aerobic conditions, and remove total nitrogen less than 50%
[0004] However, the research on this kind of aerobic denitrification bacteria has not been perfect so far, and the extensiveness of aerobic denitrification needs to be further determined.

Method used

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  • A Strain of Enterobacter cloacae and Its Application
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  • A Strain of Enterobacter cloacae and Its Application

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Embodiment 1

[0026] 1. Screening of aerobic denitrifying strains.

[0027] The activated sludge used in the present invention comes from Chongqing Jiguanshi Sewage Plant A 2 In the secondary sedimentation tank of / O process, the screening of aerobic denitrification strains adopts the plate streaking method, in which the liquid culture conditions are temperature 30-35 °C, shaking table speed 90-120rpm; solid plate culture conditions are biochemical incubator temperature 30-35 ℃, specifically follow the steps below:

[0028] 1) Inoculate 1mL of sludge taken from the secondary sedimentation tank of the sewage plant into beef extract peptone liquid medium for enrichment and culture for 24~72h, then take 1mL of the above culture solution for gradient dilution, and dilute the diluted culture solutions of different gradients respectively Inoculate into the test tubes of liquid medium Ⅰ to form several groups of culture solutions, and culture them aerobically for 24-72 hours;

[0029] 2) Measure...

Embodiment 2

[0048] Detection of the removal effect of Enterobacter cloacae HNR on TN under different carbon source conditions.

[0049] in NO 3 - -N is used as the only nitrogen source (initial concentration is 100 mg / L), C / N ratio (carbon-to-nitrogen ratio) is 10, pH is 7, and the temperature is 30°C under the aerobic conditions, choose sodium acetate, butanedi Sodium citrate, glucose, trisodium citrate, and sodium succinate were used as carbon sources, and Enterobacter cloacae HNR was used for the reaction. The results showed that strain HNR could utilize various carbon sources, especially glucose as the optimal carbon source for aerobic denitrification. When using glucose as carbon source, the removal rate of TN can reach more than 85%. See the result figure 2 .

Embodiment 3

[0051] Detection of the removal effect of Enterobacter cloacae HNR on TN under different C / N ratio conditions.

[0052] in NO 3 - -N as the only nitrogen source (initial concentration of 100 mg / L), glucose as carbon source, pH 7, temperature 30 ℃ under aerobic conditions, the effects of Enterobacter cloacae HNR at different C / N ratios were investigated respectively. TN removal effect. The results showed that strain HNR had better TN removal effect when the C / N ratio was 10-15. When the C / N ratio is 10, the TN removal rate can reach about 90%. See the result image 3 .

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Abstract

The invention discloses enterobacter cloacae with the aerobic denitrification function and an application of the enterobacter cloacae in wastewater treatment. The enterobacter cloacae are enterobacter cloacae HNR and are collected to the China General Microbiological Culture Collection Center (CMGCC) on January 13th, 2015, the collecting number is CGMCC No.10348. According to the enterobacter cloacae, under the aerobic condition, NO3<->-N can be converted into a gaseous product through the aerobic denitrification effect with organic carbon as the unique carbon source and the NO3<->-N (nitrate nitrogen) as the unique nitrogen source, and synchronous removing of nitrogen elements and the organic carbon is achieved. According to the enterobacter cloacae, the removing rate for the NO3<->-N is as high as 90% to 98 %, the removing rate for the total nitrogen can be 85% to 95 %, and the removing rate for the total organic carbon can be higher than 85%. The enterobacter cloacae can be used for effectively removing the NO3<->-N in the organic wastewater, and have the effects that the whole process is aerobic, and the nitrogen removal efficiency is high.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Enterobacter cloacae ( Enterobacter cloacae ) HNR and its applications. Background technique [0002] The traditional biological denitrification process means that denitrifying bacteria convert NO 3 - (nitrate) reduction to gaseous products. Normally, during this process, the presence of oxygen causes NO 3 - The process of being reduced as an electron acceptor is inhibited. Therefore, denitrification needs to be carried out under (anaerobic) conditions. With the deepening of research, aerobic denitrification has been paid more and more attention. It has been found that the denitrification process can be carried out not only under anaerobic (anaerobic) conditions, but also under aerobic conditions. The discovery of strains with aerobic denitrification properties, such as Alcaligenes faecalis ( Alcaligenes faecalis) , Pseudomonas putida ( Pseudomo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/16
CPCY02W10/10
Inventor 赵彬安强郭龙杰田梦汪霞
Owner CHONGQING UNIV
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