Enterobacter cloacae and application thereof

A technology of Enterobacter cloacae, cgmccno.10348, applied in the field of environmental microorganisms, can solve the problems that the research on aerobic denitrifying bacteria is not yet perfect, the total nitrogen removal rate is less than 50%, and achieve good organic carbon removal capacity and extreme tolerance good effect

Inactive Publication Date: 2015-12-02
CHONGQING UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] "Establishment of a new screening method for aerobic denitrifying bacteria and discovery of new strains", Kong Qingxin, "Journal of Applied and Environmental Biology", 2005.04 published: using intermittent aeration enrichment and potassium cyanide selection medium to screen A strain of Pseudomonas with aerobic denitrification function ( Pseudomonas sp.) Y2-1-1, the strain uses sodium citrate as carbon source and potassium nitrate as nitrogen source to reduce nitrate nitrogen from 282.0mg / L to 148.2mg / L within 5 days under aerobic conditions, and remove total nitrogen less than 50%
[0004] However, the research on this kind of aerobic denitrification bacteria has not been perfect so far, and the extensiveness of aerobic denitrification needs to be further determined.

Method used

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  • Enterobacter cloacae and application thereof
  • Enterobacter cloacae and application thereof
  • Enterobacter cloacae and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] 1. Screening of aerobic denitrification strains.

[0027] The activated sludge used in the present invention comes from Chongqing Jiguanshi Wastewater Treatment Plant A 2 / O process secondary sedimentation tank, the aerobic denitrification strains are screened by plate streak method, the liquid culture condition is 30~35℃, the shaker speed is 90~120rpm; the solid plate culture condition is the biochemical incubator temperature 30~35 ℃, specifically follow the steps below:

[0028] 1) Inoculate 1 mL of the sludge taken from the secondary sedimentation tank of the sewage treatment plant into the beef extract peptone liquid medium for enrichment and culture for 24 to 72 hours, and then take 1 mL of the above-mentioned culture solution for gradient dilution, and then separate the different gradients of the diluted culture solution Inoculate into the test tube of liquid medium Ⅰ to form several groups of culture solution, aerobic culture for 24~72h;

[0029] 2) Determine the pH va...

Embodiment 2

[0048] The effect of Enterobacter cloacae HNR on the removal of TN under different carbon source conditions was tested.

[0049] In NO 3 - -N is the only nitrogen source (the initial concentration is 100mg / L), the C / N ratio (carbon to nitrogen ratio) is 10, the pH is 7, and the temperature is 30°C under aerobic conditions, sodium acetate and succinic acid are selected respectively Sodium, glucose, trisodium citrate, and sodium succinate are used as carbon sources, and enterobacter cloacae HNR is used for the reaction. The results show that the strain HNR can use a variety of carbon sources, especially glucose as the optimal carbon source for aerobic denitrification. When glucose is used as the carbon source, the TN removal rate can reach over 85%. See the result figure 2 .

Embodiment 3

[0051] The effect of Enterobacter cloacae HNR on TN removal under different C / N ratio conditions was tested.

[0052] In NO 3 - -N is the only nitrogen source (the initial concentration is 100mg / L), glucose is the carbon source, pH is 7, and the temperature is 30℃. Under aerobic conditions, the effects of Enterobacter cloacae HNR on TN at different C / N ratios The removal effect. The results showed that strain HNR had better TN removal effect when the C / N ratio was 10-15. When the C / N ratio is 10, the TN removal rate can reach about 90%. See the result image 3 .

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Abstract

The invention discloses enterobacter cloacae with the aerobic denitrification function and an application of the enterobacter cloacae in wastewater treatment. The enterobacter cloacae are enterobacter cloacae HNR and are collected to the China General Microbiological Culture Collection Center (CMGCC) on January 13th, 2015, the collecting number is CGMCC No.10348. According to the enterobacter cloacae, under the aerobic condition, NO3<->-N can be converted into a gaseous product through the aerobic denitrification effect with organic carbon as the unique carbon source and the NO3<->-N (nitrate nitrogen) as the unique nitrogen source, and synchronous removing of nitrogen elements and the organic carbon is achieved. According to the enterobacter cloacae, the removing rate for the NO3<->-N is as high as 90% to 98 %, the removing rate for the total nitrogen can be 85% to 95 %, and the removing rate for the total organic carbon can be higher than 85%. The enterobacter cloacae can be used for effectively removing the NO3<->-N in the organic wastewater, and have the effects that the whole process is aerobic, and the nitrogen removal efficiency is high.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Enterobacter cloacae ( Enterobacter cloacae ) HNR and its applications. Background technique [0002] The traditional biological denitrification process means that denitrifying bacteria convert NO 3 - (nitrate) reduction to gaseous products. Normally, during this process, the presence of oxygen causes NO 3 - The process of being reduced as an electron acceptor is inhibited. Therefore, denitrification needs to be carried out under (anaerobic) conditions. With the deepening of research, aerobic denitrification has been paid more and more attention. It has been found that the denitrification process can be carried out not only under anaerobic (anaerobic) conditions, but also under aerobic conditions. The discovery of strains with aerobic denitrification properties, such as Alcaligenes faecalis ( Alcaligenes faecalis) , Pseudomonas putida ( Pseudo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/16
CPCY02W10/10
Inventor 赵彬安强郭龙杰田梦汪霞
Owner CHONGQING UNIV
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