1316 results about "Denitrifying bacteria" patented technology

A wastewater treatment system includes a tidal, sequential vertical flow marsh cell system having at least three marsh cells in serial forward fluid communication. Incoming wastewater to be treated is channeled to at least the first and a second marsh cell, and a portion of water exiting a final marsh cell, to the first marsh cell surface. Each marsh cell is alternately flooded and drained for enhancing aeration of incoming water and marsh cell contents, which include media and plant roots having biofilms growing thereon, the biofilms including a population of nitrifying and denitrifying bacteria, for achieving substantially simultaneous nitrification and denitrification of the wastewater.

One kind of short range nitrifying-anaerobic aminoxidizing sequencing batch type denitrifying biomembrane mode and plant is disclosed. The limited aerating mode with real-time control of the sewage temperature inside the reactor and high frequency alternation between aeration and anaeration can inhibit the growth of nitrite oxidizing bacteria and denitrifying bacteria so that the aminoxidizing bacteria and the anaerobic aminoxidizing bacteria become the dominant bacteria in the nitrifying and denitrifying stages. In the nitrifying stage, nitrification is stopped in the nitrite step; and in the denitrifying stage, ammonia nitrogen acts as the direct electron donor to reduce nitrite. The plant is provided with fixed spherical composite stuffing with spherical shell of polypropylene and filled inside biological composite material. Sectional discrete water feeding mode is adopted to feed the sewage to be treated in four stages into the reactor.

The invention relates to a method for producing a high-efficiency composite microbial agent by using livestock liquid dung, and belongs to the technical field of environmental microbiology and agricultural science. The method comprises the following steps: diluting livestock liquid dung or livestock feces with water; after hydrolysis and acidification treatment, performing standing and settlement for desilting; mixing a supernatant obtained with a photosynthetic bacterial solution for anaerobic culture; using the mixed solution as a base liquid and stepwise adding lactobacilli, saccharomyces, bacillus thuringienesis and actinomycete into the base liquid in a proportion; and after anaerobic culture and aerobic treatment, finally filling the mixture into a biological fluidized bed for further treatment to obtain the high-efficiency composite microbial agent. The microbial agent is rich in the photosynthetic bacteria, the lactobacilli, the saccharomyces, the actinomycete, the bacillus thuringienesis, azotobacter, nitrobacteria, denitrifying bacteria and other beneficial organisms, and be widely used in agriculture, livestock breeding, fresh water aquaculture, organic sewage treatment, microbial deodorization, and other aspects. The technology is suitable to be used by various livestock farms (raisers) for liquid dung treatment.

The invention discloses a microbe complexing agent with high efficiency and method using thereof for processing municipal wastewater. The microbe complexing agent contains 5-20 shares of nitrifying bacteria, 5-20 shares of denitrifying bacteria, 5-15 shares of bacillus brevis, 5-20 shares of photosynthetic bacteria, 1-20 shares of saccharomycete, each grame culture medium of single species bacteria contains 1.0-3.5 hundred million numbers of bacteria. The invention adopts improved bio-contact oxidation process with a microbe complexing agent with high efficiency as main body, adopting soft assembly complexing biology stuff as biology carrier, increaing anoxia step in a integral SBQ biochemistry treating pool to make hydrolysis and good oxygen oxidize as an integer, nitrifying and denitrifying finished in a pool, removing effectively ammonia, nitrogen and phosphor via aeration regulation and regulation of microbe reaction process. The microbe complexing agent is in characterized in high efficiency and no pollution, the waste water processing method with low cost, less invest, low operation cost, and the method has strong adaptability, less mud product, having better economy and social benefit.

The present invention relates to a wastewater treating system and method for removing pollutants of carbon, nitrogen, phosphorous, comprising: a first reactor contains methanogenic bacteria, PAO and denitrifying bacteria for eliminating the organic carbon in denitrification by denitrifying bacteria, adsorbing partial organic carbon and releasing phosphate by PAO and; metabolizing organic carbon to form methane gas by methanogenic bacteria; a second reactor disposed at the rearward of first reactor, second reactor treats the effluent from first reactor by denitrifying bacteria and PAO, denitrifying bacteria is able to denitrifying the nitrate and PAO is able to process the phosphorous releasing; a third reactor disposed rearward of second reactor, which comprises PAO, heterotrophic bacteria and nitrifying bacteria, wherein PAO is applied for phosphorous accumulating process, the heterotrophic bacteria is applied for carbon removal process, the nitrifying bacteria is applied for nitrifying process; and a membrane separation reactor disposed inside or rear of third reactor to separate various microorganisms and the treated effluent. The present invention applies methanogenic bacteria in the system for decreasing the amount of waste sludge efficiently and reduce power consuming.

The invention relates to the field of wastewater treatment, and relates to a biological degradation method of nitrogen-containing heterocyclic compound wastewater. An oxidation state compound is added in the nitrogen containing heterocyclic compound wastewater, an anaerobic environment is formed by micro aeration, and mixed electron acceptors of dissolved oxygen and the oxidation state compound simultaneously exist in the system; an upflow biological aerated filter is used as a main reaction device, an optimal degradation effect of nitrogen-containing heterocyclic compounds is achieved according to different components and concentrations of the nitrogen-containing heterocyclic compounds, the pH is controlled to 7.0-7.5, the concentration of the dissolved oxygen is controlled to 0.05-0.3 mg/L (water discharged from the upper part of the filter), and the range of the oxidation-reduction potential is controlled to 150-50 mv; under an anaerobic aeration condition, with the nitrogen-containing heterocyclic compounds as electron donors and oxygen and oxygen-containing compounds as electron acceptors, under the combined effects of micro aerobic bacteria, facultative anaerobic bacteria, aerobic/anoxic denitrifying bacteria and the like, the nitrogen-containing heterocyclic compounds are completely degraded into carbon dioxide and water.

A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

The invention relates to a compound microecological preparation used to purify aquaculture water in the field of aquiculture, a preparation form and a preparation process thereof; the preparation is composed of photosynthetic bacterium, bacillus subtilis, denitrifying bacterium, coagulant aid and auxiliary components; the preparation form is composed of an inner layer and an outer layer, wherein, the outer layer comprises bacillus subtilis, denitrifying bacterium and coagulant aid, and the inner layer comprises solid photosynthetic bacterium particles. The invention breaks through the prior theory that people keeps using nitrite bacteria and nitrobacteria to solve the problem of the accumulation of ammonia nitrogen and nitrite in the aquaculture water in the control of aquaculture water, introduces an advanced SHARON theory in wastewater treatment, integrates the characteristics of the aquaculture water quality, purifies the aquaculture water with denitrifying bacteria strains as the main bacteria strains, can quickly and effectively reduce the content of NH3 and NO2- in the aquaculture water, is not inhibited by the concentration of the NO2-, and effectively reduces the oxygen consumption in the aquaculture water.

The invention discloses an integrated pre-denitrification and denitrogenation biological filter sewerage treatment process. The integrated process is characterized in that: the process is realized by combining an anoxic denitrification biological filter and an aerobic nitrification biological filter in tandem, first, sewage with organic substances and ammonia nitrogen is mixed with part of effluent water of the aerobic nitrification biological filter which flows back to a water inlet end, then the sewage and the effluent water pass the anoxic denitrification biological filter together, and the organic substances in the sewage are taken as a carbon source by denitrifying bacteria adhesively growing on bio-ceramsite in the anoxic denitrification biological filter to reduce nitrate nitrogen from the return water of the aerobic nitrification biological filter into nitrogen gas, thus realizing denitrification function; and the effluent water of the anoxic denitrification biological filter flows to the aerobic nitrification biological filter, and a filter material in the aerobic nitrification biological filter is adhered with special growing aerobic decarbonization microbes and the growing denitrifying bacteria which further degrade the organic substances and the ammonia nitrogen from the anoxic denitrification biological filter, thus causing content indicators of the organic substances, the ammonia nitrogen, total nitrogen and the like in the effluent water of the aerobic nitrification biological filter to meet the discharge requirement.

This invention relates to method and reactor for removing organic matters, sulfides and nitrates from wastewater simultaneously. The method solves the problems of high cost and easy secondary pollution faced by the present technique in treating sulfur and nitrogen-containing wastewater. The method utilizes sulfur-autotrophic denitrifying bacteria and sulfur-heterotrophic denitrifying bacteria biological membrane to treat wastewater. The reactor is a fixed bed biological membrane reactor. The filler is lava or activated carbon. A reaction zone is formed at the middle and lower part of the inner cavity of the cylinder, while a precipitation zone is formed at the upper part. A gas collection zone is formed at the middle and lower part of the inner cavity of the gas collection chamber, while a water storage zone is formed between the bottom of the gas collection chamber and the flush weir. The method has such advantages as high treatment efficiency, no secondary pollution, low running cost, and low sludge productivity. The reactor has such advantages as small floor area and easy operation. The reactor can be continuously operated, and can be solely used or used as one treatment unit for wastewater treatment.

A method of denitrification and phosphorus removal under low temperature relates to a way of sewage treatment. The invention solves the problem of the poor effect of existing biological simultaneous denitrification and phosphorus removal under low temperature. The invention comprises the steps of: 1. cultivation and fixation for nitrifying and denitrifying bacteria of low temperature tolerance; 2 start-up response system and cultivation of phosphate accumulating bacteria ; 3. denitrification and phosphorus removal under low temperature by the steps: a. denitrification by nitrate solution refluxing; b. anaerobic action; c. aerobic phosphorus removal; d. precipitation and sludge reflux; e. contacting oxidation and nitration; f. precipitation and water obtained. After the steps, denitrification and phosphorus removal of sewage treatment under low temperature are finished. The invention can realize simultaneous denitrification and phosphorus removal under low temperature. The invention comprises the contents under low temperature: the removal rate of COD is above 85 percent; the removal rate of overall phosphorus reaches above 80 percent; the removal rate of ammonia nitrogen reaches above 70 percent when the intake ammonia nitrogen is between 25-60mg/L.

The invention provides a method for simultaneously removing phosphorous and nitrogen through the synergistic effect of nanoscale-iron and microbes. The method is characterized in that firstly limonite ore is broken and sieved so as to obtain particulate materials with the required particle size, the particulate materials mainly comprising nanoscale iron and having millimeter particle size and the porous structure characteristic are prepared through high temperature reduction under a hydrogen or carbon monoxide atmosphere, the nanoscale iron in the particulate materials slowly release nascent hydrogen atoms and hydrogen gas, and nitrate is chemically reduced; meanwhile, the particulate materials have relatively high void ratio, and abundant function anaerobic microbe denitrifying bacteria adhere to the inner parts and the outer surfaces of the particulate materials and reduce the nitrate by using hydrogen released through reaction between the nanoscale-iron and water as electron donors; adherent dependent nitrate iron-oxidizing bacteria also have the effect of reducing the nitrate; the nitrate in wastewater is removed under the chemical action of the nanoscale-iron and the synergistic effects of the anaerobic microbes; ferrous and ferric hydroxides are continuously formed on the inner surfaces and the outer surfaces of the porous particulate materials through the chemical reaction of the nanoscale-iron and water and the synergistic effect of the anaerobic microbes, so that the chemical adsorption of phosphorous in water is promoted, thus the effect of simultaneously removing phosphorous and nitrogen is achieved.

The invention discloses a method and a device for simultaneously carrying out wastewater treatment and power generation by using a photocatalysis associated microbial fuel cell technology. The device takes conductive glass coated with an n-type semiconductor photocatalyst film as an outside wall of a cathode chamber and takes conductive glass coated with an n-type semiconductor photocatalyst film as an outside wall of an anode chamber, a conductive filler respectively connected on the outside walls of the cathode chamber and the anode chamber is used as an electrode of a microbial fuel cell. The conductive filler is a carbon fiber rope for microbial growth. Denitrifying bacteria and nitrifying bacteria are respectively cultivated at the cathode and anode of the microbial fuel cell, and mainly used for the degradation of organic matters and the removal of ammonia nitrogen. Under the irradiation of ultraviolet light, electrons flow from the anode to the cathode, so that the anode has strong oxidability, and the cathode has strong reducibility. In the invention, the functions of microbial fuel cells and a photocatalysis technology are mutually associated, so that the wastewater treatment cost is low, and the generating capacity is large.

The invention describes application of pure culture microbial strains under denitrifying conditions for growth on crude oil thereby modifying crude oil properties to enhance its recovery.

The invention discloses a method for treating ammonia containing wastewater through simultaneous nitrification and denitrification. The method comprises the following steps: firstly, cultivating denitrifying granular sludge; and secondly, using the denitrifying granular sludge to treat ammonia containing wastewater through simultaneous nitrification and denitrification. The cultivating method of the denitrifying granular sludge comprises the following steps: firstly, inoculating seed sludge in an aerobic reactor, and performing accumulation culture to the aerobic sludge with high ammonia-nitrogen and low chemical oxygen demand (COD) wastewater; secondly, separating and screening aerobic denitrifying bacteria to perform domestication and cultivation; and thirdly, inoculating the domesticated and cultivated aerobic denitrifying bacteria in the denitrifying granular sludge to cultivate and obtain the finished denitrifying granular sludge. The invention adopts the domesticated and cultivated simultaneous nitrification and denitrification sludge treatment catalyst to treat ammonia containing wastewater, and has the characteristics of high removal rate of contaminants, low energy consumption and material consumption and the like; and nitrification and denitrification can be completed in the same reactor, and the short-range simultaneous nitrification and denitrification technology can be applied in actual wastewater treatment.

The invention discloses a preparation method of compound microorganisms and a recombination enzyme solid agent which can degrade the ammonia- nitrogen and nitrite in the aquicultural water environment. The invention uses universal liquid medium to culture bacillus subtilis, nitrifying bacteria, photosynthetic bacteria, denitrifying bacteria, yeast, actinomyces etc six under different temperatures. Then the culture liquid is centrifugally separated and then the bacteria body is collected. The bran is added into the bacteria body as an adsorbent. The mixture of the bacteria body and the bran is boiled and dried on a fluidized bed. And the freeze-dried powder of compound microorganisms is obtained. Then proteases, lipase, and amylase are added to make the compound microorganisms and the recombination enzyme solid agent. The invention is put into the aquicultural water that needs to be dealt with and becomes nitro-nitrogen by the decomposition of proteases, lipase, and amylase and the ammonification of bacillus subtilis. Next, the nitro-nitrogen becomes nitrogen which is released into the air. So the invention can take effect quickly on completely degrading the ammonia- nitrogen and nitrite in the aquicultural water.

The invention discloses a permeable reactive filler for removing nitrate organisms from underground water, a system and a filling method thereof, and belongs to the field of underground water pollution remediation. The filler for the permeable reactive barrier system is formed by the mixture of biological slow release carbon source materials, pH buffer nutrient element materials and bacteria enrichment water treatment fillers, the process inlet and outlet areas of the permeable reactive barrier system are filled with water treatment filters, a process reaction area is filled with the mixture of quartz sand or basalt and fillers, and the bacteria enrichment water treatment fillers inoculated with acclimatized native denitrifying bacteria. According to the invention, slow release carbon source materials, slow release pH nutrient element materials and water treatment fillers can be flexibly added, and the shortcomings that the carbon source materials are inoculated with hang membrane materials and native microorganisms in the remediation area are insufficient are avoided. The water treatment filters in the water outlet area are capable of preventing secondary pollution.

The invention relates the microorganism agent used to control oil field sulfate-reducing bacteria and its application. The invention can used to control oil field secondary depletion sulfate-reducing bacteria and microorganism. The water outlet system of secondary depletion technology comprises sulfate-reducing bacteria, H2S and sulphide, which can cause corrosion and pollution of sulphide. Microorganism agent comprises denitrifying bacteria culture, nutrescin and synergic nutrescin. 0.01-1wt% denitrifying bacteria culture, 5-100g/L culture and 10-200ppm synergic nutrescin. Microorganism agent can avoid SRB growing, SRB producing H2S, and removing sulphide. At the same time, metabolite can improve recovery efficiency.

The invention discloses a method for purifying mariculture wastewater by utilizing a compound microbial inoculant. The method comprises the following steps: (1) screening the compound microbial inoculant which consists of photosynthetic bacteria, nitrobacteria, denitrifying bacteria, candida utilis, lactobacillus acidophilus and bacillus; (2) activating the compound microbial inoculant by utilizing a culture medium: directly adding the strain into the culture medium based on 1% of inoculation amount (w/v), and activating the strain by a shaker at the temperature of 37 DEG C with the speed of 150rpm (revolution per minute) for 24 hours so that the total content of microbes is 7*10<8>-9*10<8>CFUs (colony forming units)/mL; and (3) adding the activated compound inoculant into the treated aquaculture wastewater, wherein the compound inoculant accounts for 0.06%-0.12% of the total weight of the aquaculture wastewater. The method has the advantages of no toxicity and harm on aquaculture animals, simple process, fast organic matter degradation speed, obvious effect, no drug residues and no secondary pollution. Therefore, the method has economic feasibility and is applicable to popularization and application to coastal aquaculture industry.

The invention provides a microbial carrier. The microbial carrier is in a porous solid form and contains a biodegradation material with the weight content of 50% or more, the specific surface area is 100-900 m<2>/g, the average pore size is 20 nm-0.4 mm, the microbial carrier is at least of a two-layer structure, and the average pore size of the surface layer is 50 nm-0.4 mm. The microbial carrier can provide larger living environment for microorganisms, provide a carbon source for growth and propagation of microorganisms particularly denitrifying bacteria, improve the concentration of microorganisms in unit volume and improve the microbiological treatment efficiency.

The invention belongs to the applied biology technical field, and relates to a water environmental microbiology reparation agent and a preparation method thereof. The reparation agent has the mass percent of the components in the microbiology total weight as follows: bacillus subtilis is 30-40 percent, bacillus licheniformis is 30-40 percent, ray fungi is 10-20 percent, denitrifying bacterium is 10-20 percent, and photosynthetic bacterium is 10-20 percent. The microbiology preparation of the invention has strong adaptability, can compensate the water body original bacterium when being put in water, quickly form the preponderant flora in the water body, produce the high-activity biological enzymes for quickening the decomposition of the left diet and the animal wastes, and produce the antibiotic substance to restrain the growing of harmful bacterium and reduce the occurrence of the illness as well as ensure the production of green marine products.

The invention discloses an acclimatization and screening method for heterotrophic nitrification aerobic denitrifying bacteria. According to the invention, the heterotrophic nitrification aerobic denitrifying bacteria are obtained through shaking flask enrichment, sludge accumulation, SBR reactor batch acclimation and low-DO (Dissolved Oxygen) process gradual acclimation and isolation and screening, and a method for efficiently acquiring target strains is established. According to the screening method of the invention, the heterotrophic nitrification aerobic denitrifying bacteria can be successfully screened, further, microbial preparation prepared with the screened bacteria as active components can be used for effectively depriving ammonia nitrogen and total nitrogen in the water, meanwhile, and the preparation has tolerance and degradation ability for phenols, amines, heterocyclic materials, cyanogens and polyaromatic hydrocarbons and other toxic materials in waste water, has strong accommodative ability and has great application prospect in actual engineering application.