379 results about "Denitrifying bacteria" patented technology

The invention discloses a method for denitrifying wastewater by using a low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacterium, and relates to a method for denitrifying wastewater by using a biological bacterium, the bacterium is Pseudomonas putida, is named as Pseudomonas umsongensis BK17, belongs to Pseudomonas, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under low-temperature stress, and is high in low-temperature tolerance. The strain can efficiently treat nitrogen-containing wastewater under the condition of 10 DEG C, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of domestic sewage and other water bodies in cold regions in China.

The invention discloses a composite denitrification accelerant and a preparation method thereof, and belongs to the technical field of sewage treatment. The carbon source composite material is prepared from the following raw materials in parts by weight: 30-50% of a carbon source, 2-5% of vitamins, 2-5% of amino acids, 1-3% of a biological stimulant, 1-2% of signal molecules, 1-5% of metal elements, 3-5% of a pH regulator and 25-60% of water. Through metabolites such as vitamins, amino acids and biological stimulants, growth and reproduction of denitrifying bacteria are promoted, and functions of related enzymes in the denitrification process are improved. The signal molecule optimizes the metabolic activity of bacteria by regulating and controlling the expression of genes, related to the denitrification process, of the denitrifying bacteria. The metal ions can enhance the activity of the denitrifying enzyme, so that the efficiency of denitrification treatment is improved. According to the denitrification accelerant, the denitrification process is promoted through the synergistic effect of three aspects of growth and reproduction of denitrifying bacteria, expression of denitrification genes and improvement of activity of denitrification-related enzymes.

The present application relates to the field of municipal wastewater treatment, and particularly relates to a highway service area wastewater treatment device and method based on AOOA-MBBR process. The device comprises a first anoxic tank, a first aerobic tank, a second aerobic tank and a second anoxic tank connected in sequence, and is filled with MBBR filler respectively, and the volume of the filled MBBR filler is taken as the effective volume; the first aerobic tank and the second aerobic tank are respectively provided with an aeration device; the first anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the first aerobic tank is used for inoculating hydrolytic bacteria, nitrifying bacteria and denitrifying bacteria; the second aerobic tank is used for inoculating nitrifying bacteria, and the second anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the second aerobic tank is connected with the first anoxic tank through a reflux pipe, and the reflux pipe is provided with a reflux power assembly. The present application solves the problems of existing highway service wastewater treatment with extremely low carbon-nitrogen ratio, such as the need for additional carbon source / electron donor, high operation cost, poor denitrification effect and high sludge yield.

The application discloses a method for efficient denitrification and decarburization of kitchen waste and sludge co-fermentation anaerobic digestion effluent, which removes part of COD and ammonia nitrogen in the influent sludge by using a first SNAD, and maintains a certain amount of free ammonia of a certain concentration due to the high concentration of ammonia nitrogen and the high pH of the influent, so as to maintain the inhibition effect on nitrifying bacteria and ensure the conversion of part of the ammonia nitrogen into nitrite nitrogen, and the high activity of anaerobic ammonia oxidation bacteria is ensured in the first SNAD by limiting the aeration amount, so that the ammonia nitrogen and nitrite can be further removed; the effluent enters a nitrification tank, and the ammonia nitrogen is nitrified into nitrate nitrogen under the condition of nitrifying bacteria and sufficient aeration amount, and then enters a denitrification tank, and the denitrification process is carried out under the condition of heterotrophic denitrifying bacteria and sufficient carbon source, so that most of the nitrate nitrogen in the system is converted into nitrogen gas and removed, finally, the clear liquid is discharged after being filtered by an MBR membrane in a membrane tank, so as to achieve the purpose of efficient denitrification and decarburization of kitchen waste anaerobic digestion effluent.

The invention relates to the technical field of microorganisms and particularly discloses paenibacillus mucilaginosus as well as a microbial agent and application thereof. The preservation number of the paenibacillus mucilaginosus N-002 is CGMCC (China General Microbiological Culture Collection Center) No.32752, and the preservation number of the paenibacillus mucilaginosus N-002 is CGMCC No.32752. The paenibacillus mucilaginosus N-002 provided by the invention can produce a urease inhibitor and indoleacetic acid at high yield and has no denitrification effect, the urease inhibitor produced by the paenibacillus mucilaginosus N-002 can reduce the urease activity of soil, significantly reduce the ammonia emission and nitrous oxide emission of the soil, especially after a nitrogen fertilizer is applied, is beneficial to soil nitrogen element maintenance, improves the fertilizer efficiency of the nitrogen fertilizer, and has a good application prospect. Meanwhile, the influence of emission of ammonia and nitrous oxide on the environment is reduced; the strain has no denitrification effect, and can increase the proportion of non-denitrifying bacteria in soil and reduce the denitrification effect of the soil after being applied to the soil, so that the emission of nitrous oxide in the soil is reduced, and environmental protection is facilitated.

The present invention relates to the field of urban sewage treatment, and particularly relates to an urban sewage carbon-nitrogen-phosphorus synergistic deep treatment method based on a biological manganese oxide. The method comprises: S1, configuring an upward flow denitrification filter tank; S2, a starting stage, involving: taking a mixed solution of a secondary effluent and a secondary influent of an actual urban sewage treatment plant and manganese sulfate as the influent of the denitrification filter tank, with the manganese concentration being 3.0 mg / L, inoculating hydrolytic acidification bacteria, denitrifying bacteria, anaerobic ammonium oxidation bacteria and manganese oxidation bacteria into the denitrification filter tank, and gradually increasing the ratio value of the secondary influent to the secondary effluent in the mixed solution to 3%; and S3, a stable stage, involving: taking a mixed solution of the secondary effluent and the secondary influent of the actual urban sewage treatment plant and manganese sulfate as the influent of the denitrification filter tank, wherein the manganese concentration is 3.0 mg / L, the hydraulic retention time is 3-4 hours, and the backwashing period is 9-11 days. The present invention can synchronously and efficiently remove carbon, nitrogen and phosphorus from the secondary effluent of an actual urban sewage treatment plant at a low cost.

The invention relates to the field of sewage treatment, and discloses a sewage treatment method based on desulfurization and denitrification, which comprises the following steps: S1, magnetic flocculation-electro-catalysis pretreatment: adding a polyaluminum chloride-magnetic powder composite flocculant into sewage through a magnetic flocculation reactor to form a magnetic floc, removing suspended matters through a permanent magnetic separation device, and then entering an electro-catalysis oxidation pond, carrying out electrolysis by taking titanium-based lead dioxide as an anode and active carbon fiber as a cathode; and S2, performing synchronous desulfurization and denitrification on the three-dimensional electrode fluidized bed, feeding pretreated effluent into a three-dimensional electrode fluidized bed reactor, filling activated carbon particles as a third electrode, and adding a composite flora of thiobacillus and denitrifying bacteria. The efficient removal of sulfides, total nitrogen, total phosphorus and chemical oxygen demand in the sewage is realized through a multi-stage synergistic process of magnetic flocculation-electrocatalysis pretreatment, synchronous desulfurization and denitrification of a three-dimensional electrode fluidized bed, deep denitrification and dephosphorization of a denitrification filter tank and catalytic ozonation-membrane separation combined deep treatment.

The invention belongs to the technical field of sewage treatment, and particularly relates to a low-carbon-nitrogen-ratio sewage short-process nitrogen and phosphorus removal system and method. The method comprises the following steps: S1, capturing a carbon source and synthesizing an internal carbon source; s2, synchronous denitrification phosphorus removal; S3, layered nitrification treatment and internal carbon source reutilization; and S4, material circulation. According to the invention, denitrifying phosphorus-accumulating bacteria are used as an active carbon source carrier, and soluble VFAs which is easily fight for by common heterotrophic denitrifying bacteria is converted into solid PHA in the bacteria in a carbon source capture and internal carbon source synthesis tank; according to the method, the total nitrogen and the total phosphorus in the sewage with the low carbon-nitrogen ratio can be efficiently and deeply removed, and meanwhile, the method has the outstanding advantages of being low in energy consumption, small in sludge yield and small in occupied area.

The invention relates to a composite ecological filler for enhancing in-situ denitrification of a river water body, a percolation system and application. The composite ecological filler is composed of an anoxic denitrification filler and a transition regulation filler or composed of an aerobic nitrification filler, an anoxic denitrification filler and a transition regulation filler; the aerobic nitrification filler comprises an inorganic mineral matrix denitrification filler, biological ceramsite, a mineral-based slow-release functional material and a microbial immobilization functional carrier; the anoxic denitrification filler comprises a sulfur-based composite electron donor material, a slow-release organic carbon source material, a zero-valent iron-based environmental remediation material and denitrifying bacterium embedding gel; the transition regulation and control filler comprises a multifunctional water treatment filter material, activated carbon and an environment response type functional microbial carrier. The invention also provides application of the composite ecological filler and a percolation system, solves the problem that the traditional denitrification filler cannot cooperatively support nitrification / denitrification due to single function, and also solves the problem that the fixed ratio of the traditional denitrification filler is difficult to adapt to dynamic fluctuation of water quality.

The invention provides a functional aggregate, a preparation method thereof and a self-purification asphalt pavement material. The preparation method of the functional aggregate comprises the following steps: screening residual mud and residue soil, crushing and screening to obtain a raw material, or mixing low-iron-content residue soil and red mud to prepare the raw material; the preparation method comprises the following steps: heating raw materials to 400-550 DEG C, introducing CO2, carrying out heat preservation for 40-120 minutes, then heating to 750-850 DEG C, and carrying out heat preservation for 40-120 minutes, so as to obtain calcined aggregate; soaking the mixed material in a mixed solution containing 0.1-1mol / L FeCl3 for more than 10 hours, and then carrying out hydrothermal reaction at 100-150 DEG C for 4-8 hours to obtain aggregate; and dipping the aggregate in a bacterial liquid containing denitrifying bacteria, petroleum hydrocarbon degrading bacteria and a curing agent, and obtaining the functional aggregate by adopting a vacuum negative pressure dipping method. The material formed by compounding the functional aggregate and the asphalt modifier has the functions of adsorption, catalytic oxidation and biodegradation.

The invention discloses a filler reinforced ecological floating bed for purifying aquaculture tail water and application thereof, and belongs to the technical field of water treatment. The filler reinforced ecological floating bed comprises canna cultivated in a floating plate and straw or straw biochar suspended below the floating plate; the straw biochar is obtained by taking straw as a raw material and pyrolyzing the straw at 500 DEG C or 700 DEG C. The ecological floating bed capable of purifying the aquaculture tail water is prepared by utilizing the emergent aquatic plant canna which has high purification efficiency on nitrogen and phosphorus and the straw or straw biochar which has a large surface area and is beneficial to the growth of denitrifying bacteria, and the ecological floating bed can effectively reduce the content of nitrogen and phosphorus in the aquaculture tail water, accelerate the denitrification and dephosphorization rate and improve the quality of the aquaculture tail water. The tail water purification method can be used for reducing nitrate accumulation, realizing tail water purification, providing scientific basis and technical support for tail water treatment work in aquaculture, and being beneficial to promoting green and sustainable development of aquaculture.

The invention relates to the field of sewage treatment, in particular to pseudomonas stutzeri and application of the pseudomonas stutzeri in nitrogen-containing sewage treatment. The invention provides Pseudomonas stutzeri, and the preservation number of the Pseudomonas stutzeri is CGMCC (China General Microbiological Culture Collection Center) No.25807. The invention also provides a preparation method of the Pseudomonas stutzeri. The invention provides a method for screening heterotrophic nitrification-aerobic denitrification bacteria for treating aquaculture tail water and evaluating the denitrification performance of the heterotrophic nitrification-aerobic denitrification bacteria, which is suitable for treating the heterotrophic nitrification-aerobic denitrification bacteria of the aquaculture tail water, and eliminates adverse factors of traditional biological denitrification bacteria in the prior art. The strain is separated from activated sludge in Qiu Lake of Hainan University, and has remarkable denitrification capacity.

The invention discloses a sewage treatment equipment state supervision system, belongs to the technical field of sewage treatment, and solves the problems that the sewage contains reducing sulfides and competes with denitrifying bacteria for electron donors, so that the COD in an anoxic zone is sufficient, the nitrate reduction rate is low, a false carbon source sufficient trap with inhibited denitrification occurs, but the sulfide is easy to oxidize, and the potential safety hazard is caused. And a sampling detection result is distorted and is difficult to identify. According to the invention, sewage is measured in an anoxic zone through a probe interface module, under the scheduling of a time division control module, a constant potential rectifier module excites sulfide characteristic oxidation current in a DPV time period, a logarithmic current detection module captures a peak value and integrates a charge quantity, an impedance detection module measures phase offset in an EIS time period, a signal processing module fuses characteristic values, and a temperature compensation module eliminates errors. The reference electrode protection module ensures signal fidelity, outputs sulfide concentration and electron competition index, starts a dosing system when the concentration exceeds the limit, and prevents sampling oxidation distortion in the whole process.

The invention discloses a method for realizing deep autotrophic nitrogen removal of sludge digestion liquid based on alternate intermittent aeration of a double-membrane aeration bio-membrane reactor (MABR). The process device comprises a sludge digestion liquid raw water tank, a single-stage double-MABR reactor, two MABR devices and a water outlet tank. According to the method, sludge digestion liquid enters the single-stage double-MABR reactor, the two MABR devices are alternately and intermittently aerated, the state of one aeration and one aeration stopping is always kept, and ammonia nitrogen in raw water is converted into nitrite through ammonia oxidizing bacteria enriched on the inner side of an MABR membrane. And simultaneously supplying to anaerobic ammonium oxidation bacteria enriched on the outer sides of the two MABR membranes through matrix diffusion for denitrification and generation of nitrate. Denitrifying bacteria enriched on the outer side of the MABR membrane utilize raw water organic matters to drive short-cut denitrification to synchronously supply nitrite to anaerobic ammonium oxidation bacteria so as to realize deep autotrophic nitrogen removal. According to the invention, a double-anaerobic ammonia oxidation reaction hot area is constructed through alternate intermittent aeration of double MABRs, and efficient deep autotrophic nitrogen removal of the sludge digestion liquid can be stably realized.

The invention provides a sewage enhanced nitrogen and phosphorus removal process based on microbial community interaction, and belongs to the field of sewage treatment. The method comprises the steps of sewage pretreatment, anaerobic phosphorus release, anoxic denitrification, aerobic nitrification phosphorus uptake and the like, an anaerobic-anoxic-aerobic three-section reactor is adopted, and proper ecological nichs are provided for phosphorus-accumulating bacteria, denitrifying bacteria and nitrifying bacteria by accurately regulating and controlling parameters such as dissolved oxygen, hydraulic retention time and carbon nitrogen ratio of each section, so that the phosphorus-accumulating bacteria, the denitrifying bacteria and the nitrifying bacteria can be efficiently degraded, and the phosphorus-accumulating bacteria, the denitrifying bacteria and the nitrifying bacteria can be efficiently degraded. The competition problem of biological nitrogen removal and biological phosphorus removal is effectively relieved, the nitrogen and phosphorus removal efficiency is remarkably improved, the total nitrogen removal rate reaches 85% or above, and the total phosphorus removal rate exceeds 90%; straw-based modified charcoal is adopted as a carrier, so that the loading capacity and activity of functional flora are improved, and flora interaction is enhanced; meanwhile, resource utilization of agricultural wastes is realized, and the method has remarkable environmental benefits and economic advantages.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

The invention discloses an iron powder-pyrite coupled algal-bacterial symbiosis sewage treatment device and method.The iron powder-pyrite coupled algal-bacterial symbiosis sewage treatment device comprises an algal-bacterial symbiosis reactor body, an iron powder-pyrite assembly, a water inlet pump, an aeration system and a PLC control system; an inclined plate settling zone, a sludge settling zone and a reactor water outlet are arranged at the top, conversion of organic matters and ammonia nitrogen is realized through photosynthetic oxygen production, effluent from the top of the algal-bacterial symbiotic reactor is pumped into an iron powder-pyrite assembly by an external circulating pump, nitrate is reduced into nitrogen through sulfur autotrophic denitrifying bacteria, and phosphorus is removed through iron flocculent precipitation. The invention has the following beneficial effects: the algal-bacterial symbiotic system can utilize light energy to carry out photosynthesis, does not need a large amount of aeration, and reduces aeration energy consumption; the iron powder is combined with a sulfur autotrophic denitrification system, so that the long-term operation performance of pyrite autotrophic denitrification can be effectively improved, nitrogen and phosphorus removal is enhanced, and generation of sulfate radicals is reduced.

The utility model provides a PVP (Polyvinyl Pyrrolidone) production wastewater treatment device and wastewater treatment equipment. A pre-anoxic tank, an anaerobic tank, an anoxic tank and an aerobic tank of the PVP production wastewater treatment device are sequentially communicated, a baffle is arranged in a fluid chamber of the pre-anoxic tank and divides the fluid chamber into a first reflux chamber and a second reflux chamber, a first reflux stirrer is arranged in the first reflux chamber, and a second reflux stirrer is arranged in the second reflux chamber. A rotary backflow blade of the first backflow stirrer is arranged towards the direction of the water outlet, the second backflow stirrer is arranged in the second backflow chamber, and a rotary backflow blade of the second backflow stirrer is arranged towards the direction of the water inlet, so that water flow circularly flows in the first backflow chamber and the second backflow chamber in the direction from the water inlet to the water outlet; the water flow sequentially passes through the first phosphorus-accumulating denitrifying bacterium filler piece arranged in the first backflow chamber and the second phosphorus-accumulating denitrifying bacterium filler piece arranged in the second backflow chamber. According to the PVP production wastewater treatment device, the nitrogen and phosphorus removal effect of the device is enhanced by improving the pre-anoxic tank.

The invention relates to the technical field of paper cups, and discloses a compound bacterium for degrading nitrite in an aquaculture water body, the compound bacterium is composed of nitrifying bacteria, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist, the nitrifying bacteria are mixed flora of autotrophic ammonia oxidizing bacteria and heterotrophic nitrifying bacteria, and the penetrant is a mixture of bacillus coagulans, denitrifying bacteria, bacillus coagulans, a penetrant and a synergist. The denitrifying bacteria comprise a synergistic effect system of the Pseudomonas (Pseudomonas) and the Paracoccus (Paracoccus), and the Pseudomonas (Pseudomonas) and the Paracoccus (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas)) are The dual-path directional conversion of nitrite is realized through the synergistic effect of constructed nitrification-denitrification synergistic flora and a functional aid (polyethylene glycol-SDS-L-glutamine system). Parameter control shows that in a water body (NO2 <-> is equal to 2.5 mg / L) in the later culture period of the penaeus vannamei boone, the degradation rate reaches 81.4% within 48 hours, and the nitrate increment is controlled within 12%. The technology breaks through the bottleneck of failure of a traditional microbial agent in a high-salinity (35 per mill) and high-density culture environment, and is suitable for sea and fresh water whole-variety aquaculture.

The present invention relates to a method for treating landfill leachate. The steps include: mixing nitrogen-containing landfill leachate and activated sludge and then introducing the mixture into a denitrification tank. Under the condition of inhibiting nitrous oxide reduction, nitrite in the mixture generates nitrous oxide gas dissolved in the sewage under the action of denitrifying bacteria in the denitrification tank; then introducing it into an ammonification tank for gas-liquid separation to collect nitrous oxide gas; and obtaining the sewage after ammonification treatment under the ammonification action of ammonifying functional bacteria in the ammonification tank; further introducing it into a nitrification tank to obtain sewage containing nitrite under the action of nitrosifying bacteria; refluxing to the denitrification tank for cyclic reaction; and then introducing it into a separator for separation to obtain an effluent that can be discharged and sewage that needs to be recycled for treatment. The present invention also relates to a treatment system capable of cooperating with the above treatment method. It changes the existing treatment process of directly discharging nitrogen into the air, turns to collecting nitrous oxide gas, shortens the treatment time, reduces greenhouse gas emissions, and realizes energy recovery.

The invention discloses a preparation method of a pesticide pollution remediation agent for groundwater of karst landforms, and relates to the technical field of groundwater pollution remediation. The method has the effect of enhancing pesticide degradation, pesticide pollutants can be efficiently degraded in a complex environment of karst underground water by compounding hard water-resistant fungi, dehalogenation bacteria and denitrifying bacteria, and a better remediation effect is achieved; the medicament can improve the stability and the adsorption capacity of the carrier, the bionic calcium phosphate nano carrier is adopted, the specific surface area is high, the adsorption capacity is excellent, the adsorption capacity on pollutants can be effectively enhanced, and the remediation effect is improved; the agent has an efficient repairing mechanism, the load capacity of the flora is enhanced through biological induction mineralization and physical adsorption enhancement of a bacterium-carrier complex, the release rate of the flora is controlled through an intelligent coating technology, and precise repairing is achieved.

The invention relates to a CdS / denitrifying bacteria hybrid system as well as a construction method and application thereof, and relates to the technical field of water nitrate pollution treatment. Comprising composite denitrifying bacteria, Zn-doped CdS heterojunction nanoparticles loaded on the surface of the composite denitrifying bacteria, and an electronic mediator anchored at the interface of the heterojunction nanoparticles and a flora, the composite denitrifying bacteria are prepared from Stutzeria balearica CXK-1 and Stutzeria balearica GUA-1 according to the volume ratio of the Stutzeria balearica CXK-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 being 1 to (0.8 to 1.2). The hybrid system can utilize photoelectrons to drive microbial denitrification under an illumination condition, so that the reduction of nitrate radicals is realized, and the effect of water treatment is achieved.

The utility model discloses a water distributor for integrated sewage treatment equipment. The water distributor comprises a main water inlet pipe, a water distribution main pipe and a plurality of branch pipe groups, the main water inlet pipe extends in the Z direction, and the bottom of the main water inlet pipe is communicated with the water distribution main pipe; the water distribution main pipe is of a square frame structure formed by mutually connecting two transverse pipes and two longitudinal pipes; the plurality of branch pipe groups are sequentially arranged in the water distribution main pipe square frame along the X direction or the Y direction, each branch pipe group comprises a branch pipe and a plurality of homogeneous dispersion pipes uniformly distributed on the branch pipe, two ends of the branch pipe are communicated with the water distribution main pipe, the upper end of each homogeneous dispersion pipe is communicated with the branch pipe, and the lower end of each homogeneous dispersion pipe is closed; and water outlet holes are annularly distributed in the outer wall of the homogeneous dispersion pipe. Sewage and denitrifying bacteria in the tank can be fully fused, so that the reaction rate of the denitrification process is greatly improved, and the reaction time is shortened.

The invention relates to the technical field of immobilized microorganisms and wastewater treatment, in particular to immobilized microorganism gel beads, a preparation method and application. The pellets are prepared by the preparation method. The preparation method comprises the following steps: S1, mixing a denitrifying bacteria suspension and a mixed gel solution, and adding diatomite to obtain a mixed bacteria solution; s2, dropwise adding the mixed bacterial liquid into a calcium salt solution, and carrying out a first cross-linking reaction to obtain immobilized microorganism balls; and S3, adding the pretreated immobilized microorganism balls into an aluminum sulfate aqueous solution, and carrying out a second cross-linking reaction to obtain the immobilized microorganism gel balls. The application refers to the application of the small ball in wastewater treatment or sewage treatment. According to the invention, the immobilized microbial gel beads with high mechanical strength and good stability can be prepared with low foam and low energy consumption.

This invention discloses a continuous-flow anaerobic bypass IFAS-AOA device and method for treating inert high-ammonia nitrogen wastewater, belonging to the field of biological treatment technology for high-ammonia nitrogen wastewater. The device consists of three parts: a raw water storage tank, a continuous-flow anaerobic bypass AOA reactor, and a sludge-water separation system. The effluent from the end of the anaerobic process enters the aerobic zone of the AOA reactor, while the anaerobic end-mixed liquor bypasses to the anoxic zone, mixes with the aerobic end-effluent, flows through the anoxic zone, and enters the sludge-water separation system. In the anaerobic zone, endogenous denitrifying bacteria store organic matter from the raw water as an intracellular carbon source and bypass to the anoxic zone. In the aerobic zone, short-cut nitrification coupled with anaerobic ammonia oxidation serves as the main nitrogen removal pathway. In the anoxic zone, anaerobic ammonia oxidation coupled with endogenous denitrification achieves deep nitrogen removal. The existence of anaerobic bypass allows some of the mixed liquor to bypass the aerobic zone and directly bypass to the anoxic zone, thus fully utilizing the carbon source in the raw water. This invention can achieve stable short-cut nitrification and effective retention of anaerobic ammonia oxidizing bacteria, showing broad application prospects.

This utility model discloses an oxygen denitrification treatment device for aquaculture water, including a treatment tank. The treatment tank is equipped with a filtration zone, an aerobic biological zone, an anoxic biological zone, and a degassing zone. The filtration zone is filled with quartz sand filter media. The aerobic biological zone contains biological packing material and has a jet aeration ring on its outer wall, which is connected to an ozone-oxygen mixture generator. The anoxic biological zone is filled with porous ceramsite packing material. The degassing zone has a guide plate inside. In this utility model, the aquaculture water can be filtered for suspended particulate matter through the quartz sand filter media. The jet aeration ring can introduce ozone-oxygen mixture gas, which, together with the honeycomb biological packing material, can complete ammonia nitrogen oxidation. The denitrifying bacteria on the surface of the porous ceramsite packing material can convert nitrates into nitrogen gas. Finally, the gas is separated by the guide plate, and the purified water can be discharged from the outlet. Thus, through the above structure, efficient denitrification, oxygenation, and purification of aquaculture water can be achieved, improving water quality treatment efficiency and stability.

The application discloses a biological electrochemical auxiliary sulfur conversion promoting sulfur-based denitrification and sulfate emission reduction method and belongs to the field of water treatment. ‑ Nitrogen-containing wastewater enters an elemental sulfur denitrification system, and NO3 0 is reduced to N2 by sulfur autotrophic denitrifying bacteria, and elemental sulfur (S 2‑ ) is converted into SO4 2‑ . Effluent enters a cathode chamber of an electrochemical auxiliary sulfate reduction system, and hydrogen autotrophic sulfate reducing bacteria utilize cathode hydrogen production to reduce SO4 ‑ to HS 2‑ , and meanwhile, alkalinity produced by cathode hydrogen production neutralizes acidity produced in the denitrification process in the elemental sulfur denitrification system. ‑ HS 2‑ / supernatant is returned to the elemental sulfur denitrification system to react with S 0 to generate polysulfide (S n 2‑ ), S n 2‑ , which is used as an efficient electron donor to participate in denitrification, thereby significantly improving denitrification efficiency. The application does not need to add an organic carbon source, sludge production is small, high-concentration nitrogen-containing wastewater is efficiently treated, and the content of sulfate in effluent is simultaneously reduced, thereby realizing sulfur resource recycling, and the application is an economic and environment-friendly autotrophic denitrification method.

This invention discloses a bioelectrochemical denitrification system using a slow-release carbon source as the anode fuel, belonging to the field of microbial fuel cell technology. It comprises an anode chamber, a cathode chamber, an anode, a cathode, wires, and a separating membrane between the anode and cathode chambers. Electrochemically active microorganisms grow on the anode in the anode chamber, and anaerobic fermentation bacteria are present in the solution. An electrolyte and hydrogel particles with slow-release carbon function are added to the anode chamber. Electrochemically active denitrifying bacteria grow on the cathode in the cathode chamber. The hydrogel-released carbon serves as the solid fuel at the anode, providing a continuous electron donor to the cathode. Simultaneously, the selective permeation membrane controls the transmembrane transport of small organic molecules to the cathode, providing a carbon source for the denitrifying bacteria. The combined supply of electrons and carbon enhances denitrification by the denitrifying microorganisms at the cathode. This system is suitable for enhanced denitrification of polluted water bodies with low C / N ratios.

The present invention relates to a salt-tolerant heterotrophic nitrifying and denitrifying bacterial strain, its cultivation method, and application. The strain addresses the technical problem of existing nitrifying and denitrifying bacteria inhibiting nitrification in the presence of a carbon source. The strain is named Pseudomonas stutzeri YXH-102 and is deposited with the General Microbiology Center of the China National Center for Microbiological Culture Collection under the accession number CGMCC No. 27688. The present invention can be used in the field of sewage denitrification treatment.

The application provides a biological denitrification treatment method for low-carbon-nitrogen-ratio wastewater, and utilizes single-stage nitrification and denitrification to treat ammonia-nitrogen wastewater in a one-piece submerged biological filter (SBAF reactor). The method comprises the following steps: sludge inoculation, sludge biofilm formation, system starting, and stable operation. In the sludge inoculation, sludge meeting the microbial community distribution is selected. In the system starting process, the microbial community distribution in the sludge in the one-piece submerged biological filter (SBAF reactor) is used as the symbol of successful system starting. During the input of the high-concentration ammonia-nitrogen wastewater to be treated, the aerobic nitrifying bacteria and the anaerobic denitrifying bacteria can more stably meet the above microbial community distribution, the system has better stability, the stable operation time is longer, the sludge does not need to be replaced for restarting during the stable operation, the continuous operation time is longer, can reach at least 250 days or more, and the frequency of replacing the sludge for restarting is reduced.