441 results about "Denitrifying bacteria" patented technology

The invention relates to the technical field of water treatment, in particular to a denitrification slow-release composite carbon source as well as a preparation method and application thereof. Comprising the following components in percentage by weight: 2-10% of hydroxy acrylic acid, 2-10% of saccharides, 5-15% of straw waste liquid, 5-15% of kitchen waste liquid, 2-10% of saccharides, 3-15% of porous volcanic ash, 5-15% of polyvinyl alcohol, 0.2-1% of inorganic salt, 1-2% of polypeptide, 0.5-1% of growth factors, 0.2-1.5% of MFC agar, 0.1-0.5% of cellulase, 0.1-0.5% of protease, 0.1-0.5% of denitrifying bacteria and the balance of water. The sum of the percentages of the raw materials is 100%. The invention has the advantages that: each nutrient component is balanced, the nutrition is comprehensive, the activity of denitrifying bacteria is improved, the number of species and community layers of a biological system of a sewage treatment plant are increased, the growth of various microorganisms is facilitated, nutrient substances can be fully utilized, and the denitrification effect is far higher than that of related carbon sources such as single methanol, glucose and the like.

The invention discloses a method for denitrifying wastewater by using a low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacterium, and relates to a method for denitrifying wastewater by using a biological bacterium, the bacterium is Pseudomonas putida, is named as Pseudomonas umsongensis BK17, belongs to Pseudomonas, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under low-temperature stress, and is high in low-temperature tolerance. The strain can efficiently treat nitrogen-containing wastewater under the condition of 10 DEG C, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of domestic sewage and other water bodies in cold regions in China.

The invention discloses a composite denitrification accelerant and a preparation method thereof, and belongs to the technical field of sewage treatment. The carbon source composite material is prepared from the following raw materials in parts by weight: 30-50% of a carbon source, 2-5% of vitamins, 2-5% of amino acids, 1-3% of a biological stimulant, 1-2% of signal molecules, 1-5% of metal elements, 3-5% of a pH regulator and 25-60% of water. Through metabolites such as vitamins, amino acids and biological stimulants, growth and reproduction of denitrifying bacteria are promoted, and functions of related enzymes in the denitrification process are improved. The signal molecule optimizes the metabolic activity of bacteria by regulating and controlling the expression of genes, related to the denitrification process, of the denitrifying bacteria. The metal ions can enhance the activity of the denitrifying enzyme, so that the efficiency of denitrification treatment is improved. According to the denitrification accelerant, the denitrification process is promoted through the synergistic effect of three aspects of growth and reproduction of denitrifying bacteria, expression of denitrification genes and improvement of activity of denitrification-related enzymes.

The present application relates to the field of municipal wastewater treatment, and particularly relates to a highway service area wastewater treatment device and method based on AOOA-MBBR process. The device comprises a first anoxic tank, a first aerobic tank, a second aerobic tank and a second anoxic tank connected in sequence, and is filled with MBBR filler respectively, and the volume of the filled MBBR filler is taken as the effective volume; the first aerobic tank and the second aerobic tank are respectively provided with an aeration device; the first anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the first aerobic tank is used for inoculating hydrolytic bacteria, nitrifying bacteria and denitrifying bacteria; the second aerobic tank is used for inoculating nitrifying bacteria, and the second anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the second aerobic tank is connected with the first anoxic tank through a reflux pipe, and the reflux pipe is provided with a reflux power assembly. The present application solves the problems of existing highway service wastewater treatment with extremely low carbon-nitrogen ratio, such as the need for additional carbon source / electron donor, high operation cost, poor denitrification effect and high sludge yield.

The invention provides an environment-friendly microbial strain and a water treatment agent preparation process, and relates to the field of water treatment. The environment-friendly microbial strain is prepared from the following raw materials in parts by weight: 20 parts of nitrifying bacteria, 20 parts of denitrifying bacteria, 35 parts of bacillus and 25 parts of EM bacteria, the nitrifying bacteria comprise nitrosomas and nitrobacteria, and the denitrifying bacteria comprise nitrosomas and nitrobacteria. The denitrifying bacteria comprise denitrifying bacilli and sternobacter, the bacillus comprises bacillus subtilis and bacillus licheniformis, and the EM bacteria comprise rhodopseudomonas, lactococcus lactis and saccharomyces cerevisiae. Through flora screening and culture, the culture efficiency is improved, through a fermentation process, fermentation liquor overflow and insufficient dissolved oxygen caused by excessive foam are effectively prevented, the stability of the fermentation process is ensured, through preparation preparation, a good packaging material and a sealing technology, moisture absorption and pollution of a microbial inoculum are prevented, and the quality and safety of a product are ensured.

This invention discloses a method for remediating groundwater containing organic pollutants, comprising the following steps: 1) Extracting groundwater containing organic pollutants using multiple extraction pumps, adding ferrous salts at a solid-liquid ratio of 1:50-200, mixing thoroughly, and adjusting the pH of the system to 6-8 to obtain intermediate treatment solution one; 2) Adding a remediation agent to the obtained intermediate treatment solution one, mixing and stirring for 3-6 hours to obtain intermediate treatment solution two; 3) Passing the obtained intermediate treatment solution two sequentially into an anaerobic reactor and an aerobic tank for treatment, and discharging after reaching the required standards; the packing material of the anaerobic reactor contains microorganisms, including at least two or more of denitrifying bacteria, Bacillus coagulans, Bacillus subtilis, Bacillus clonorchis, Bacillus indusiata, and Serratia marcescens. The remediation method provided by this invention has high remediation efficiency, thorough removal of organic matter, and is economically feasible, with an organic matter removal rate of over 98%.

The invention relates to the technical field of denitrification fillers, in particular to an autotrophic denitrification filler for denitrification and a preparation method thereof. The autotrophic denitrification filler comprises the following raw materials in parts by weight: 80-100 parts of sulfur powder, 8-12 parts of zinc carbonate powder, 8-12 parts of clay, 4-6 parts of ferric oxide, 4-6 parts of polyhydroxyalkanoate, 4-6 parts of sugarcane cellulose, 1-3 parts of cotton, 1-3 parts of a binder and 1-3 parts of a cross-linking agent. According to the method, in the high-frequency ultrasonic treatment, the sulfur powder and sodium sulfide are in non-uniform contact, so that non-uniform sunken dissolving positions can be generated on the surface of the sulfur powder, the specific surface area of the sulfur powder is effectively increased, and after the sulfur powder is used as a main raw material to prepare the filler, the filler has a relatively large contact reaction area with sewage; a good attachment and growth environment is provided for denitrifying bacteria, and the purification effect of the autotrophic denitrification filler for denitrification is improved.

The invention discloses a salt-tolerant denitrifying bacterium and application of the salt-tolerant denitrifying bacterium, the salt-tolerant denitrifying bacterium is specifically Stutzerimonas degrandans and is named as Stutzerimonas degrandans BP, the preservation number of the salt-tolerant denitrifying bacterium is GDMCC No.64297, and the salt-tolerant denitrifying bacterium is preserved in the Guangdong Microbial Culture Collection Center on January 19, 2024. When the Stutzerimonas degrandans BP provided by the invention is used for biological nitrogen removal of high-salinity wastewater, the Stutzerimonas degrandans BP has relatively strong salt-tolerant denitrification capability, NO3 <->-N in a high-salinity water body can be effectively removed, no by-products such as NO2 <->-N and NH4 < + >-N are accumulated, and secondary pollution cannot be caused. Experimental results show that the Stutzerimonas degrandans BP provided by the invention is a facultative anaerobic bacterium, and is suitable for biological nitrogen removal in an anaerobic or anoxic environment. Under an anaerobic condition, high-efficiency salt-tolerant denitrification can be realized by virtue of relatively small biomass (OD600 = 0.15-0.25), so that the yield of sludge can be reduced while the operation cost of the process is reduced.

The invention belongs to the field of industrial wastewater treatment, and relates to an iron autotrophic denitrification coupling anaerobic ammonia oxidation combined enrichment culture device and method. Comprising a water inlet tank, a No.1 anoxic reactor, an intermediate tank and a No.2 anaerobic reactor, the water inlet tank is provided with a water outlet pipeline of the water inlet tank, and the water outlet pipeline of the water inlet tank is connected with the No.1 anoxic reactor; the anoxic reactor I is provided with a water outlet pipeline of the anoxic reactor I, and the water outlet pipeline of the anoxic reactor I is connected with the intermediate tank; the intermediate tank is provided with an intermediate tank water outlet pipeline, and the intermediate tank water outlet pipeline is connected with the No.2 anaerobic reactor; the No.2 anaerobic reactor is provided with a No.2 anaerobic reactor water outlet pipeline; the No.2 anaerobic reactor water outlet pipeline is connected with the No.1 anoxic reactor. According to the invention, collaborative culture of autotrophic denitrification bacteria and anaerobic ammonium oxidation bacteria can be realized, an autotrophic denitrification effect provides a certain amount of nitrite and iron ions to promote anaerobic ammonium oxidation reaction, and an anaerobic ammonium oxidation effect can effectively prevent an autotrophic denitrification tank body from being mineralized.

The present invention proposes a MABR membrane assembly, a MABR reactor, a MABR reactor operation method and a MABR biofilm optimization control method, which adopts a cross-flow working mode between horizontal membrane filaments and water flow to improve the mass transfer efficiency of the matrix and metabolites in the MABR biofilm; the composite structure membrane assembly is used to promote the complementarity and symbiosis of various microorganisms such as carbon removal aerobic bacteria, nitrifying bacteria, denitrifying bacteria and anaerobic ammonia oxidizing bacteria; different areas such as MABR membrane reaction area, pre-reaction area and water distribution and gas distribution area are constructed in the same reactor to complete short-range nitrification or partial short-range nitrification reaction, short-range denitrification reaction and anaerobic ammonia oxidation reaction respectively, so as to achieve efficient biological denitrification of sewage with low carbon-nitrogen ratio in a single-stage MABR reactor. At the same time, the optimization control method of the MABR biofilm solves the problems that the overly thick biofilm in the MABR reactor is not easy to be effectively washed, and the fixed gas washing frequency and intensity are difficult to simultaneously adapt to the biofilm washing and film removal requirements of membrane filaments in different parts.

The application discloses a method for efficient denitrification and decarburization of kitchen waste and sludge co-fermentation anaerobic digestion effluent, which removes part of COD and ammonia nitrogen in the influent sludge by using a first SNAD, and maintains a certain amount of free ammonia of a certain concentration due to the high concentration of ammonia nitrogen and the high pH of the influent, so as to maintain the inhibition effect on nitrifying bacteria and ensure the conversion of part of the ammonia nitrogen into nitrite nitrogen, and the high activity of anaerobic ammonia oxidation bacteria is ensured in the first SNAD by limiting the aeration amount, so that the ammonia nitrogen and nitrite can be further removed; the effluent enters a nitrification tank, and the ammonia nitrogen is nitrified into nitrate nitrogen under the condition of nitrifying bacteria and sufficient aeration amount, and then enters a denitrification tank, and the denitrification process is carried out under the condition of heterotrophic denitrifying bacteria and sufficient carbon source, so that most of the nitrate nitrogen in the system is converted into nitrogen gas and removed, finally, the clear liquid is discharged after being filtered by an MBR membrane in a membrane tank, so as to achieve the purpose of efficient denitrification and decarburization of kitchen waste anaerobic digestion effluent.

The invention relates to the technical field of microorganisms and particularly discloses paenibacillus mucilaginosus as well as a microbial agent and application thereof. The preservation number of the paenibacillus mucilaginosus N-002 is CGMCC (China General Microbiological Culture Collection Center) No.32752, and the preservation number of the paenibacillus mucilaginosus N-002 is CGMCC No.32752. The paenibacillus mucilaginosus N-002 provided by the invention can produce a urease inhibitor and indoleacetic acid at high yield and has no denitrification effect, the urease inhibitor produced by the paenibacillus mucilaginosus N-002 can reduce the urease activity of soil, significantly reduce the ammonia emission and nitrous oxide emission of the soil, especially after a nitrogen fertilizer is applied, is beneficial to soil nitrogen element maintenance, improves the fertilizer efficiency of the nitrogen fertilizer, and has a good application prospect. Meanwhile, the influence of emission of ammonia and nitrous oxide on the environment is reduced; the strain has no denitrification effect, and can increase the proportion of non-denitrifying bacteria in soil and reduce the denitrification effect of the soil after being applied to the soil, so that the emission of nitrous oxide in the soil is reduced, and environmental protection is facilitated.

The present invention relates to the field of urban sewage treatment, and particularly relates to an urban sewage carbon-nitrogen-phosphorus synergistic deep treatment method based on a biological manganese oxide. The method comprises: S1, configuring an upward flow denitrification filter tank; S2, a starting stage, involving: taking a mixed solution of a secondary effluent and a secondary influent of an actual urban sewage treatment plant and manganese sulfate as the influent of the denitrification filter tank, with the manganese concentration being 3.0 mg / L, inoculating hydrolytic acidification bacteria, denitrifying bacteria, anaerobic ammonium oxidation bacteria and manganese oxidation bacteria into the denitrification filter tank, and gradually increasing the ratio value of the secondary influent to the secondary effluent in the mixed solution to 3%; and S3, a stable stage, involving: taking a mixed solution of the secondary effluent and the secondary influent of the actual urban sewage treatment plant and manganese sulfate as the influent of the denitrification filter tank, wherein the manganese concentration is 3.0 mg / L, the hydraulic retention time is 3-4 hours, and the backwashing period is 9-11 days. The present invention can synchronously and efficiently remove carbon, nitrogen and phosphorus from the secondary effluent of an actual urban sewage treatment plant at a low cost.

The invention discloses an enzyme source particle carrier and a preparation method and application thereof, and relates to the technical field of microbial carriers, the enzyme source particle carrier comprises denitrification inner layer particles, a nitride, activated carbon, water, a polyurethane prepolymer, an initiator and a catalyst; the denitrification inner layer particles comprise the following components: a denitrifying substance, activated carbon, a polyurethane prepolymer, an initiator and a catalyst. The enzyme source particle carrier provided by the invention enriches denitrifying bacteria and nitrifying bacteria at the same time, and adopts activated carbon to increase bacterial attachment sites in the particle carrier, so that the enzyme source particle carrier has better biological activity, mechanical strength and service life, and can better adapt to impact of complex water bodies, water quality and water quantity; and the nitrifying bacteria and the denitrifying bacteria are reasonably distributed in the enzyme source particle carrier, so that synchronous nitrification and denitrification are easily realized, and an efficient, energy-saving and carbon-source-saving denitrification process is achieved.

The invention discloses an autotrophic nitrogen removal and detoxification device and method for treating coking wastewater through two-stage low-oxygen short-cut nitrification, sulfur autotrophic denitrification and anaerobic ammonia oxidation, and belongs to the field of sewage treatment. The device comprises a water inlet tank, a sequencing batch reactor, a middle water tank and an up-flow anaerobic sludge blanket reactor. The method comprises the following steps: enabling the coking wastewater which is subjected to carbon capture and contains ammonia nitrogen and thiocyanate to enter a sequencing batch reactor, oxidizing the ammonia nitrogen into nitrite under a limited oxygen condition, and generating nitrogen, sulfate and ammonia nitrogen from the thiocyanate and the nitrite by autotrophic denitrifying bacteria; reaction effluent mixed with coking wastewater enters an upflow anaerobic sludge blanket, and anaerobic ammonium oxidation bacteria convert ammonia nitrogen and nitrite into nitrogen and generate a small amount of nitrate; and the residual nitrate, nitrite and thiocyanate are continuously and thoroughly removed through the sulfur autotrophic denitrification effect. The method provides a new idea for economical and efficient autotrophic nitrogen removal and detoxification of the coking wastewater containing thiocyanate and ammonia nitrogen.

The present invention provides an autotrophic denitrification filter material, comprising three types of materials A, B, and C, and the weight ratio of each material is A: B: C = (10-15): (1-2): 1, wherein A is a composite denitrification ingredient, including ferrous sulfide, sulfur, sodium thiosulfate and iron powder; B is an adhesive; and C is a molecular sieve treated with a microbial culture solution. The high efficiency of the autotrophic denitrification filter material with high efficiency denitrification provided by the present invention is mainly based on the following two options: first, the filter material belongs to a multivalent composite sulfide-iron coupling denitrification scheme, and the diverse electron donors of the autotrophic denitrifying bacteria have synergistic synergy; second, the autotrophic denitrifying bacteria culture solution is pre-placed in the gap of the molecular sieve, which can be slowly released, ensuring that the autotrophic denitrifying bacteria can still maintain growth when the environment is subjected to various shocks. The autotrophic denitrification filter material provided by the present invention has a simple preparation process, is easy to operate during application, and can maintain high efficiency in denitrification capacity.

The invention relates to the field of sewage treatment, and discloses a sewage treatment method based on desulfurization and denitrification, which comprises the following steps: S1, magnetic flocculation-electro-catalysis pretreatment: adding a polyaluminum chloride-magnetic powder composite flocculant into sewage through a magnetic flocculation reactor to form a magnetic floc, removing suspended matters through a permanent magnetic separation device, and then entering an electro-catalysis oxidation pond, carrying out electrolysis by taking titanium-based lead dioxide as an anode and active carbon fiber as a cathode; and S2, performing synchronous desulfurization and denitrification on the three-dimensional electrode fluidized bed, feeding pretreated effluent into a three-dimensional electrode fluidized bed reactor, filling activated carbon particles as a third electrode, and adding a composite flora of thiobacillus and denitrifying bacteria. The efficient removal of sulfides, total nitrogen, total phosphorus and chemical oxygen demand in the sewage is realized through a multi-stage synergistic process of magnetic flocculation-electrocatalysis pretreatment, synchronous desulfurization and denitrification of a three-dimensional electrode fluidized bed, deep denitrification and dephosphorization of a denitrification filter tank and catalytic ozonation-membrane separation combined deep treatment.

The invention belongs to the technical field of sewage treatment, and particularly relates to a low-carbon-nitrogen-ratio sewage short-process nitrogen and phosphorus removal system and method. The method comprises the following steps: S1, capturing a carbon source and synthesizing an internal carbon source; s2, synchronous denitrification phosphorus removal; S3, layered nitrification treatment and internal carbon source reutilization; and S4, material circulation. According to the invention, denitrifying phosphorus-accumulating bacteria are used as an active carbon source carrier, and soluble VFAs which is easily fight for by common heterotrophic denitrifying bacteria is converted into solid PHA in the bacteria in a carbon source capture and internal carbon source synthesis tank; according to the method, the total nitrogen and the total phosphorus in the sewage with the low carbon-nitrogen ratio can be efficiently and deeply removed, and meanwhile, the method has the outstanding advantages of being low in energy consumption, small in sludge yield and small in occupied area.

The invention belongs to the technical field of biological filters, and relates to an anaerobic denitrification reactor and a method for accelerating degradation of nitrate nitrogen in micro-polluted water, and the reactor mainly comprises a biological filter, a water inlet tank, a peristaltic pump and a water outlet tank. The biological filter is composed of a filler composed of a modified polyvinylidene fluoride hollow fiber membrane and a high-temperature treatment iron wire, and the wastewater treatment process of the biological filter comprises a biofilm culturing stage and a domestication stage. In the biofilm culturing stage, the activated sludge enters the biofilter along with inlet water, and is loaded on the surface of membrane filaments to form a layer of biofilm; in the domestication stage, the wastewater slowly enters a biological filter and stays in a reactor, iron and nitrate nitrogen in the wastewater are subjected to denitrification reaction under the action of denitrifying bacteria, and the purpose of rapidly removing the nitrate nitrogen is achieved. According to the method, nitrate nitrogen in the wastewater can be rapidly reduced under the condition of not additionally adding a carbon source, so that the treatment process is low-carbon, aeration is not needed, energy is saved, and the method is suitable for industrial popularization.

The invention relates to a composite ecological filler for enhancing in-situ denitrification of a river water body, a percolation system and application. The composite ecological filler is composed of an anoxic denitrification filler and a transition regulation filler or composed of an aerobic nitrification filler, an anoxic denitrification filler and a transition regulation filler; the aerobic nitrification filler comprises an inorganic mineral matrix denitrification filler, biological ceramsite, a mineral-based slow-release functional material and a microbial immobilization functional carrier; the anoxic denitrification filler comprises a sulfur-based composite electron donor material, a slow-release organic carbon source material, a zero-valent iron-based environmental remediation material and denitrifying bacterium embedding gel; the transition regulation and control filler comprises a multifunctional water treatment filter material, activated carbon and an environment response type functional microbial carrier. The invention also provides application of the composite ecological filler and a percolation system, solves the problem that the traditional denitrification filler cannot cooperatively support nitrification / denitrification due to single function, and also solves the problem that the fixed ratio of the traditional denitrification filler is difficult to adapt to dynamic fluctuation of water quality.

The invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and application thereof, the heterotrophic-aerobic denitrifying bacterium C-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC), is classified and named as brevundimonas, has the preservation number of CGMCC No.31729, and is preserved on August 23, 2024. The heterotrophic-aerobic denitrifying bacterium C-3 has the advantages that the preservation number is CGMCC No.31729, and the preservation date is 2024. The heterotrophic-aerobic denitrifying brevundimonas vesicularis C-3 (Brevundimonas vesicularis C-3) provided by the invention takes sodium acetate as a carbon source, and under the aerobic conditions that the C / N mass ratio is 10, the pH value is 7, the temperature is 30 DEG C and the rotating speed is 200r / min, a relatively good nitrate nitrogen removal effect is achieved, and the removal effect is 100%; in actual aquaculture tail water, nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total nitrogen can be well removed; meanwhile, the strain has high safety and has good application value in treatment of aquaculture tail water.

The invention provides a functional aggregate, a preparation method thereof and a self-purification asphalt pavement material. The preparation method of the functional aggregate comprises the following steps: screening residual mud and residue soil, crushing and screening to obtain a raw material, or mixing low-iron-content residue soil and red mud to prepare the raw material; the preparation method comprises the following steps: heating raw materials to 400-550 DEG C, introducing CO2, carrying out heat preservation for 40-120 minutes, then heating to 750-850 DEG C, and carrying out heat preservation for 40-120 minutes, so as to obtain calcined aggregate; soaking the mixed material in a mixed solution containing 0.1-1mol / L FeCl3 for more than 10 hours, and then carrying out hydrothermal reaction at 100-150 DEG C for 4-8 hours to obtain aggregate; and dipping the aggregate in a bacterial liquid containing denitrifying bacteria, petroleum hydrocarbon degrading bacteria and a curing agent, and obtaining the functional aggregate by adopting a vacuum negative pressure dipping method. The material formed by compounding the functional aggregate and the asphalt modifier has the functions of adsorption, catalytic oxidation and biodegradation.

The invention belongs to the field of industrial wastewater treatment, and relates to a combined enrichment culture device and method for autotrophic denitrifying bacteria. The device comprises a water inlet tank, a No.1 anaerobic reactor, a reflux tank and a No.2 anoxic reactor, the water inlet tank is provided with a water outlet pipeline of the water inlet tank, and the water outlet pipeline of the water inlet tank is connected with the No.1 anaerobic reactor; the first anaerobic reactor is provided with a first anaerobic reactor water outlet pipeline, and the first anaerobic reactor water outlet pipeline is connected with the reflux tank; a return tank water outlet pipeline is arranged at the upper part of the return tank, a return tank return pipeline is arranged at the bottom of the return tank, the return tank water outlet pipeline is connected with the second anoxic reactor, and the return tank return pipeline is connected with the first anaerobic reactor; a second anoxic reactor reflux pipeline is arranged at the bottom of the second anoxic reactor and is connected with the lower part of the reflux tank. The anaerobic ammonia oxidation reaction and the denitrification reaction promote each other, so that the anaerobic ammonia oxidation reaction can be quickly started, and the sludge leakage phenomenon is effectively avoided.

The invention discloses a filler reinforced ecological floating bed for purifying aquaculture tail water and application thereof, and belongs to the technical field of water treatment. The filler reinforced ecological floating bed comprises canna cultivated in a floating plate and straw or straw biochar suspended below the floating plate; the straw biochar is obtained by taking straw as a raw material and pyrolyzing the straw at 500 DEG C or 700 DEG C. The ecological floating bed capable of purifying the aquaculture tail water is prepared by utilizing the emergent aquatic plant canna which has high purification efficiency on nitrogen and phosphorus and the straw or straw biochar which has a large surface area and is beneficial to the growth of denitrifying bacteria, and the ecological floating bed can effectively reduce the content of nitrogen and phosphorus in the aquaculture tail water, accelerate the denitrification and dephosphorization rate and improve the quality of the aquaculture tail water. The tail water purification method can be used for reducing nitrate accumulation, realizing tail water purification, providing scientific basis and technical support for tail water treatment work in aquaculture, and being beneficial to promoting green and sustainable development of aquaculture.

The invention relates to the field of sewage treatment, in particular to pseudomonas stutzeri and application of the pseudomonas stutzeri in nitrogen-containing sewage treatment. The invention provides Pseudomonas stutzeri, and the preservation number of the Pseudomonas stutzeri is CGMCC (China General Microbiological Culture Collection Center) No.25807. The invention also provides a preparation method of the Pseudomonas stutzeri. The invention provides a method for screening heterotrophic nitrification-aerobic denitrification bacteria for treating aquaculture tail water and evaluating the denitrification performance of the heterotrophic nitrification-aerobic denitrification bacteria, which is suitable for treating the heterotrophic nitrification-aerobic denitrification bacteria of the aquaculture tail water, and eliminates adverse factors of traditional biological denitrification bacteria in the prior art. The strain is separated from activated sludge in Qiu Lake of Hainan University, and has remarkable denitrification capacity.

The invention discloses a sewage treatment equipment state supervision system, belongs to the technical field of sewage treatment, and solves the problems that the sewage contains reducing sulfides and competes with denitrifying bacteria for electron donors, so that the COD in an anoxic zone is sufficient, the nitrate reduction rate is low, a false carbon source sufficient trap with inhibited denitrification occurs, but the sulfide is easy to oxidize, and the potential safety hazard is caused. And a sampling detection result is distorted and is difficult to identify. According to the invention, sewage is measured in an anoxic zone through a probe interface module, under the scheduling of a time division control module, a constant potential rectifier module excites sulfide characteristic oxidation current in a DPV time period, a logarithmic current detection module captures a peak value and integrates a charge quantity, an impedance detection module measures phase offset in an EIS time period, a signal processing module fuses characteristic values, and a temperature compensation module eliminates errors. The reference electrode protection module ensures signal fidelity, outputs sulfide concentration and electron competition index, starts a dosing system when the concentration exceeds the limit, and prevents sampling oxidation distortion in the whole process.

The invention discloses a method for realizing deep autotrophic nitrogen removal of sludge digestion liquid based on alternate intermittent aeration of a double-membrane aeration bio-membrane reactor (MABR). The process device comprises a sludge digestion liquid raw water tank, a single-stage double-MABR reactor, two MABR devices and a water outlet tank. According to the method, sludge digestion liquid enters the single-stage double-MABR reactor, the two MABR devices are alternately and intermittently aerated, the state of one aeration and one aeration stopping is always kept, and ammonia nitrogen in raw water is converted into nitrite through ammonia oxidizing bacteria enriched on the inner side of an MABR membrane. And simultaneously supplying to anaerobic ammonium oxidation bacteria enriched on the outer sides of the two MABR membranes through matrix diffusion for denitrification and generation of nitrate. Denitrifying bacteria enriched on the outer side of the MABR membrane utilize raw water organic matters to drive short-cut denitrification to synchronously supply nitrite to anaerobic ammonium oxidation bacteria so as to realize deep autotrophic nitrogen removal. According to the invention, a double-anaerobic ammonia oxidation reaction hot area is constructed through alternate intermittent aeration of double MABRs, and efficient deep autotrophic nitrogen removal of the sludge digestion liquid can be stably realized.

The invention provides a sewage enhanced nitrogen and phosphorus removal process based on microbial community interaction, and belongs to the field of sewage treatment. The method comprises the steps of sewage pretreatment, anaerobic phosphorus release, anoxic denitrification, aerobic nitrification phosphorus uptake and the like, an anaerobic-anoxic-aerobic three-section reactor is adopted, and proper ecological nichs are provided for phosphorus-accumulating bacteria, denitrifying bacteria and nitrifying bacteria by accurately regulating and controlling parameters such as dissolved oxygen, hydraulic retention time and carbon nitrogen ratio of each section, so that the phosphorus-accumulating bacteria, the denitrifying bacteria and the nitrifying bacteria can be efficiently degraded, and the phosphorus-accumulating bacteria, the denitrifying bacteria and the nitrifying bacteria can be efficiently degraded. The competition problem of biological nitrogen removal and biological phosphorus removal is effectively relieved, the nitrogen and phosphorus removal efficiency is remarkably improved, the total nitrogen removal rate reaches 85% or above, and the total phosphorus removal rate exceeds 90%; straw-based modified charcoal is adopted as a carrier, so that the loading capacity and activity of functional flora are improved, and flora interaction is enhanced; meanwhile, resource utilization of agricultural wastes is realized, and the method has remarkable environmental benefits and economic advantages.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

The invention discloses an iron powder-pyrite coupled algal-bacterial symbiosis sewage treatment device and method.The iron powder-pyrite coupled algal-bacterial symbiosis sewage treatment device comprises an algal-bacterial symbiosis reactor body, an iron powder-pyrite assembly, a water inlet pump, an aeration system and a PLC control system; an inclined plate settling zone, a sludge settling zone and a reactor water outlet are arranged at the top, conversion of organic matters and ammonia nitrogen is realized through photosynthetic oxygen production, effluent from the top of the algal-bacterial symbiotic reactor is pumped into an iron powder-pyrite assembly by an external circulating pump, nitrate is reduced into nitrogen through sulfur autotrophic denitrifying bacteria, and phosphorus is removed through iron flocculent precipitation. The invention has the following beneficial effects: the algal-bacterial symbiotic system can utilize light energy to carry out photosynthesis, does not need a large amount of aeration, and reduces aeration energy consumption; the iron powder is combined with a sulfur autotrophic denitrification system, so that the long-term operation performance of pyrite autotrophic denitrification can be effectively improved, nitrogen and phosphorus removal is enhanced, and generation of sulfate radicals is reduced.